Morphology tissue than frozen tissue. Medical search. Frequent questions

The cell morphology is fibroblast-like. (coriell.org)
Human cells exhibiting fibroblast morphology isolated from normal pancreatic tissue. (neuromics.com)
Under an optical microscope, the control cells exhibited a spindle‑shaped, fibroblast‑like morphology. (spandidos-publications.com)

The PAXgene Tissue miRNA Kit provides purification of total RNA, including RNA from approximately 18 nucleotides, from tissues fixed and stabilized in PAXgene Tissue Containers. (qiagen.com)

By using commercially available deep learning tools (Aiforia ® ) to develop and validate a microglia morphology model, we have been able to establish a workflow for results with fewer limitations in application and reproducibility. (frontiersin.org)
The purpose of this study was to quantitatively characterize the spatiotemporal pattern of microglia morphology during the evolution of cerebral injury after IS and reperfusion. (biomedcentral.com)
Quantitative analysis reveals a significant spatiotemporal relationship between microglia morphology and evolving cerebral injury in the ipsilateral hemisphere after IS and reperfusion. (biomedcentral.com)

To overcome these issues, we use commercially available deep learning tools Aiforia ® Cloud (Aifoira Inc., Cambridge, MA, United States) to quantify microglial morphology and cell counts from histopathological slides of Iba1 stained tissue sections. (frontiersin.org)
Importantly, quantitative analyses of microglial morphology and activity are feasible and, in future studies, would assist in the comprehensive identification and stratification of their dichotomous contribution toward cerebral injury and recovery during IS and reperfusion. (biomedcentral.com)

Tissue morphology and cell viability were also evaluated. (lu.se)
Cell viability was reduced after freezing. (lu.se)
five primary scab isolates, four of nine frozen cultures and all six lyophilized strains have demonstrable viability. (who.int)
The present study was performed to evaluate the extracts of Cimicifugae Rhizoma on the morphology and viability of human stem cells derived from gingiva. (spandidos-publications.com)
Within the limits of this study, Cimicifugae Rhizoma influenced the viability of stem cells derived from the gingiva, and its direct application onto oral tissues may have adverse effects at high concentrations. (spandidos-publications.com)
The aim of the present study was to evaluate the effects of extracts of Cimicifugae Rhizoma on the morphology and viability of human stem cells derived from gingiva. (spandidos-publications.com)
Before freezing and 24 h after thawing, cell viability was assessed using live-dead assay. (huji.ac.il)
Within randomly orientated PCL-PLA fibers, HeLa cells exhibited typical shape and attachment with higher than 90% viability prior to freezing. (huji.ac.il)
Histological markers for proliferation and apoptosis also confirm the viability of tissues maintained in culture. (houstonmethodist.org)

The arrangement and function of octopus arm musculature and connective tissue. (curiouscook.com)
Individual fronds are surrounded by connective tissue, each having a central vascularized core. (medscape.com)
However, healing tendons are unlike and contain a loose connective tissue. (lu.se)
Cell renewal can occur quite intensively: for example, connective tissue cells in the pancreas are replaced every 24 hours, gastric mucosa cells - every three days, leukocytes - every 10 days, skin cells - every six weeks, approximately 70 g of proliferating small intestine cells are removed from the body daily [1]. (vechnayamolodost.ru)
Osseointegration, as applied to dental implants, refers to the postinsertion result in which medullary bone has grown up to or into the material of the implant without interposition of a connective tissue layer between the bone and the alloplastic implant material. (medscape.com)

Many antigens can be successfully demonstrated in formalin-fixed paraffin-embedded tissue sections. (wikipedia.org)
The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive cross-linking and degradation found in formalin-fixed tissues. (qiagen.com)

In the third study, the effects were evaluated of hot-air drying (AD), freeze drying (FD) and refractance-window drying (RWD) on the color (L*, a*, b*, and E), glass transition, specific heat, and surface morphology of 4 fruits and 3 vegetables. (illinois.edu)
The structure and surface morphology of CS-Ag composite hydrogels were characterized by UV-Vis, FTIR, SEM, and XRD. (preprints.org)

Tissue preparation or fixation is essential for the preservation of cell morphology and tissue architecture. (wikipedia.org)
In order to enable both molecular and traditional pathology testing from the same specimen, a method is needed for stabilization of molecular content and preservation of morphology. (qiagen.com)
Preservation of tissue morphology. (qiagen.com)

After obtaining the biopsy, place the tissue flat on a piece of firm paper/cardboard before placing in fixation medium. (medscape.com)
Current tissue fixation methods used in traditional histology are of limited use for molecular analysis. (qiagen.com)
During tissue fixation, storage, and processing, cross-links lead to degradation of nucleic acids. (qiagen.com)
PAXgene Tissue Containers provide tissue fixation for histopathology studies and enable purification of high-quality nucleic acids from the same sample for molecular analysis. (qiagen.com)
The PAXgene Tissue FIX Container (50 ml) enables fixation and stabilization of tissue specimens (up to 4 samples with a maximum size of 4 x 15 x 15 mm placed into 4 standard tissue cassettes, or a single tissue sample with a maximum size of 20 x 20 x 20 mm). (qiagen.com)
After fixation, PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. (qiagen.com)
Specifications for fixation and storage conditions in PAXgene Tissue FIX and PAXgene Tissue STABILIZER were determined using animal tissues. (qiagen.com)
The methods for tissue fixation currently used in traditional histology are of limited use for molecular analysis. (qiagen.com)
Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. (qiagen.com)
The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. (qiagen.com)
PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis. (qiagen.com)
But recovery of intact RNA from syncytium dissected by LCM is complicated due to extended steps of fixation, tissue preparation, embedding and sectioning. (biomedcentral.com)

The system consists of a tissue collection device (the PAXgene Tissue Container for collection, stabilization, storage, and transportation of human tissue specimens) and kits for purification of miRNA, total RNA, or DNA. (qiagen.com)
Frozen sections of tissue and cytological specimens are also suitable for laser microdissection. (concordia.ca)
The preparation procedure of tissue specimens requires sectioning by microtome (FFPE) or cryostat (fresh frozen) followed by mounting the tissue section onto a conductive MSI compatible carrier. (theratype.com)

Nucleic acids, proteins, and morphology of the sample are stable up to 7 days at room temperature, and for longer periods at 2-8°C, or even at -20°C and -80°C. Stabilized samples can be embedded in paraffin for histological studies. (qiagen.com)

The culture was initiated using explants of minced gingival tissue. (coriell.org)
In addition to evaluation of the patient's underlying health, it is important to assess for preexisting periodontal disease as peri-implantitis, a periodontal condition similar to periodontitis that is associated with gingival tissue inflammation, pocket formation, and ultimately loss of bone that can lead to implant failure. (medscape.com)

Under these conditions, tissues should be rapidly fresh frozen in liquid nitrogen and cut with a cryostat. (wikipedia.org)
For a cell block, take 1 to 3 x 107 cells, spin down and wash three times with PBS, then resuspend the last cell pellet in 1 ml OCT, immerse end of tube into liquid nitrogen to snap freeze. (utsouthwestern.edu)

Mass spectrometry imaging (MSI) is a unique analytical technique enabling a qualitative and quantitative label-free MS analysis of multiple biomolecules in the context of tissue morphology. (theratype.com)

Together, our findings should motivate caution when interpreting conditional alleles, and suggest the exciting possibility of inter-tissue RNA or protein trafficking in modulation of reproductive biology. (elifesciences.org)

The second study was undertaken to examine changes in the drying and drying rate curves, moisture diffusivity, and microstructures of fresh and spoiled plant tissues. (illinois.edu)
The results demonstrated that drying spoiled plant tissues would be less time consuming and potentially less energy intensive than drying fresh ones. (illinois.edu)
In plants, phytoplasmas colonize the cytoplasm of vascular phloem cells that transport nutrients to growing plant tissues (Pagliari et al. (biomedcentral.com)

PAXgene Tissue FIX Container (50 ml) is only to be used in conjunction with PAXgene Tissue STABILIZER. (qiagen.com)

However, flow cytometry can be less effective at detecting extremely rare cell populations, and there is a loss of architectural relationships in the absence of a tissue section. (wikipedia.org)
citation needed] The enzyme-linked immunosorbent assay or ELISA is a diagnostic method for quantitatively or semi-quantitatively determining protein concentrations from blood plasma, serum or cell/tissue extracts in a multi-well plate format (usually 96-wells per plate). (wikipedia.org)
Cell populations are offered at passage 1 in a frozen vial. (neuromics.com)
The Applied Biosystems ArcturusXT is a microdissection system (LCM) that uses UV and IR lasers to capture the isolated cell without changing morphology or integrity of a biological tissue. (concordia.ca)
The morphology of the cells was viewed under an inverted microscope and the analysis of cell proliferation was performed using a Cell Counting kit‑8 (CCK‑8) assay on days 1, 3, 5 and 7. (spandidos-publications.com)
RC is a cell line with lymphoblast-like morphology that was isolated from the pleural effusion of a 71-year-old, White, female with lymphoma. (atcc.org)
Dr. Shen's team has developed emulsion microfluidic technology for creating MOS, tiny, nanoliter-sized basal membrane extract (BME) droplets composed of tissue cell mixtures which can be generated at a rapid pace from an automated device. (technologynetworks.com)
Within natural diffusion limit of vascularized tissue, tumor-derived MOS allowed sufficient penetration by therapeutic immune T-cells such as CAR-T, enabling a novel T cell potency assay to assess tumor killing by the engineered T-cells. (technologynetworks.com)
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
During cell division, tissue rejuvenation occurs: new cells take the place of damaged ones, while repair (elimination of DNA damage) occurs more intensively and regeneration is possible in case of tissue damage. (vechnayamolodost.ru)
Involved in the regulation of both adhesion and cell morphology and cancer progression. (joplink.net)
We prefer to do a cell block and then frozen sections instead of cytospins. (utsouthwestern.edu)
To remove the frozen cell block, warm end of tube slightly by holding it, then pop the side of the tube against side of cryostat chamber. (utsouthwestern.edu)
The addition of cryoprotectors to semen extenders improves cell survival after the freezing process. (iranjournals.ir)
Cryosurvival requires that cell freezing and thawing be done within certain biophysical and biological limits defined by the following cryobiology principles: cells should be frozen in such a way that little or none of their intracellular water freezes. (iranjournals.ir)
Live cell morphology and process activity were measured from movies acquired in acute brain slices from GFP-CX3CR1 transgenic mice after IS and 24-h reperfusion. (biomedcentral.com)
The extension of the necrotic core into the penumbra is influenced by additional factors such as regional differences in the composition of brain tissue, the vulnerability of different cell types to ischemia, residual tissue perfusion and additional events such as reperfusion [ 2 - 4 ]. (biomedcentral.com)
The classical morphological assessment of tissues and cells is an important step towards a precise categorization of molecular changes at the level of individual tissue and cell types. (theratype.com)
Moreover, for genes that are expressed in multiple tissues or at multiple stages of development, the use of tissue-specific expression of the Cre recombinase allows gene function to be perturbed in specific cell types and/or at specific times. (elifesciences.org)
The disparate functions of different cell types are coordinated by a wide array of mechanisms, including direct cell-cell contacts within a tissue, shared access to nutrient pools, and both local (paracrine) and longer-range trafficking of small molecule and peptide signals. (elifesciences.org)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

In this review, we focus on the bioactive and mechanical characteristics that natural hydrogels may possess to allow efficient production of organs and tissues for biomedical applications, emphasizing the reinforcement techniques to improve their biomechanical properties. (preprints.org)
Currently, clinical trials are underway of drugs that selectively eliminate old cells in organs and tissues, thereby preventing degenerative changes in organs and cancer. (vechnayamolodost.ru)
Stem cells that exist in almost all organs and tissues are able to divide indefinitely. (vechnayamolodost.ru)

3D bioprinting techniques have brought considerable innovation in biomedicine, especially in the field of tissue engineering, allowing the production of 3D organ and tissue models for in vivo transplantation purposes or for in-depth and precise in vitro analyses. (preprints.org)
Our approach allows pixel-accurate, objective, semantic-based and functional tissue analyses. (theratype.com)

Testis morphology was analyzed using light and electron microscopy. (biomedcentral.com)
Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)

All results were consistent across multiple depth layers in this stratified squamous epithelial tissue. (houstonmethodist.org)
The p63 protein, a homologue of p53, may be associated with tumor formation in the epithelial tissue, acting as an oncogene 11,12 . (bvsalud.org)

citation needed] Western blotting allows the detection of specific proteins from extracts made from cells or tissues, before or after any purification steps. (wikipedia.org)
PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample. (qiagen.com)

Maternal obesity alters placental tissue function and morphology with a corresponding increase in local inflammation. (aging-research.group)

Treatment with higher sucrose concentrations minimizes the risk of RNA degradation, whereas longer incubation times help maintaining the morphology of tissue sections. (biomedcentral.com)

The disadvantages of frozen sections include poor morphology, poor resolution at higher magnifications, difficulty in cutting over paraffin sections, and the need for frozen storage. (wikipedia.org)
Tissue samples fixed and stored in PAXgene Tissue Containers can be paraffin-embedded and used for pathological studies as well as for subsequent purification of miRNA, RNA, and/or DNA. (qiagen.com)

Directional solidification demonstrates benefits in adhered cells and native tissues cryopreservation through controlled heat transfer. (huji.ac.il)
The results suggest that directional freezing becomes a viable option for cryopreservation in tissue engineering applications. (huji.ac.il)

This is more time consuming but it give better cellular morphology. (utsouthwestern.edu)

Significantly higher yeast and mold counts (log CFU/g) were found in the spoiled tissues, causing tissue softening and deterioration. (illinois.edu)
The TGA drying curves showed that spoiled plant tissue dried significantly faster (up to 30%) than its fresh counterpart, with a higher effective moisture diffusivity. (illinois.edu)

Our results are consistent with a role of EMT in most if not all ovarian cancer metastases and demonstrate that identical morphologies between primary and metastatic cancer samples is insufficient evidence to negate a role of EMT in the metastatic process. (biomedcentral.com)

Eight reference based and 12 de novo assembled transcriptomes were produced, for juvenile shoot tissue from both species. (biomedcentral.com)
Glycerol is the cryoprotector often used to freeze semen of different species. (iranjournals.ir)

The left (treated) and right (control) tibialis anterior muscle (LTA, RTA) was excised, weighed, sectioned, quick-frozen, and stored at -80 degrees C. Transverse sections (12 µm) were cut, mounted on pre-coated microscope slides, and stained using a routine procedure with Harris Hematoxylin & Eosin. (cdc.gov)

To enable both molecular and traditional pathology testing from the same specimen, a method is needed to stabilize molecular content and preserve morphology. (qiagen.com)

Natural-derived hydrogels, especially those obtained from the decellularization of biological tissues, are promising bioinks for 3D printing purposes, as they present the best biocompatibility characteristics. (preprints.org)

Our broad range of tissue processors means you can choose the right instrument for your laboratory's space, throughput, and workflow needs. (leicabiosystems.com)

The use of high-resolution MSI enables the in situ detection and spatial imaging of hundreds to thousands of metabolites with minimum demands on FFPE tissue sample volumes. (theratype.com)

the levels of urea, creatinine, and trypsin in the plasma as well as intercellular adhesion molecule-1 (ICAM-1), receptor of advanced glycation end-products (RAGE), tumor necrosis factor- α (TNF- α ), malondialdehyde (MDA), lactic acid (LA), and 2,3-DPG in the renal tissue were increased in the shock group after 3 h of hypotension. (hindawi.com)
The most common indication for a frozen section is to determine whether surgical margins are free of tumor (ie, to assess the adequacy of tissue excision). (medscape.com)
Another clinically relevant example for the use of immunohistochemical stainings for tissue annotation are pan-cytokeratine as an epithelial marker to first annotate potential tumor cells and HER2/neu stainings to then identify HER2/neu postive and negative tumor regions (images on the left) - an information important for optimal therapy choice. (theratype.com)

The hydrogel samples were made by using the freeze‑thawing method, and they were examined using the Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), porosity amusement, swelling ratio and keratin release kinetic models that analyzed using (zero-order, first-order Higuchi and Korsmeyer-Peppas models). (preprints.org)
The fiber size and morphology was characterized using scanning electron microscopy. (huji.ac.il)

We investigated the effect of various sucrose concentrations as cryoprotectant on RNA quality and morphology of syncytial sections. (biomedcentral.com)

Quantitative analysis was performed using image cytometry with systematic random sampling (percentage immunopositive/total tissue area). (bmj.com)

The use of optimal sucrose concentrations as cryoprotection plays a key role in RNA stability and morphology of sections. (biomedcentral.com)

By comparing microRNA levels between mouse medulloblastoma and normal cerebellar tissues, we identified a set of down-regulated microRNAs including miR-31. (oncotarget.com)

Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps the most commonly applied immunostaining technique. (wikipedia.org)
However, limited information is currently available regarding the effects of Cimicifugae Rhizoma on dental tissue, including mesenchymal stem cells derived from gingiva. (spandidos-publications.com)
Unlike with conventional organoids, MOS can be directly infected with viruses without the removal and suspension of cells from its surrounding BME scaffold, hence recapitulating the process of viral infection of the host tissue. (technologynetworks.com)
As novel tissue engineered constructs (TECs) are developed, current tissue banking practices need better control over ice formation and growth to prevent cryodamage to cells and a scaffold. (huji.ac.il)
While up to 80% of HeLa cells were alive on fiber mats after freezing using DMSO with or without directional solidification step. (huji.ac.il)
The demonstrated controlled freezing may assist optimizing the freezing of more sensitive cells. (huji.ac.il)
In our body, there are renewing tissues in which there is a pool of constantly dividing cells that replace spent or dying cells. (vechnayamolodost.ru)
Tissue regeneration occurs due to the proliferation of stem cells, which can not only divide, but also differentiate into cells of the tissue whose regeneration is taking place. (vechnayamolodost.ru)
Stem cells are present in the myocardium, in the brain (in the hypocampus and in the olfactory bulbs) and in other tissues. (vechnayamolodost.ru)
Microglia cells continuously survey the healthy brain in a ramified morphology and, in response to injury, undergo progressive morphological and functional changes that encompass microglia activation. (biomedcentral.com)

Tendon tissue storage and preconditioning are often used in biomechanical experiments and whether this generates alterations in tissue properties is essential to know. (lu.se)
Significant alterations in morphology were noted in the 100 and 1,000 µg/ml groups when compared with the control group. (spandidos-publications.com)

PAXgene Tissue Containers are dual-chamber containers prefilled with 2 reagents. (qiagen.com)

2016) offers enormous unexploited potential for tissue-based research and molecular diagnostics. (theratype.com)

We investigate the use of live tissue culture experiments to serve as a surrogate for in vivo metabolic measurements. (houstonmethodist.org)
To validate this approach, NADH and FAD fluorescence intensity and lifetime images were acquired with a two-photon microscope from hamster cheek pouch epithelia in vivo, from biopsies maintained in live tissue culture up to 48 h, and from flash-frozen and thawed biopsies. (houstonmethodist.org)
This study suggests that short-term ex vivo tissue culture may be more appropriate than frozen-thawed tissue for optical metabolic and morphologic measurements that approximate in vivo status. (houstonmethodist.org)
Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)

For purification of total RNA including miRNA, the system requires the use of PAXgene Tissue Containers for tissue collection and stabilization, followed by RNA isolation and purification using the PAXgene Tissue miRNA Kit. (qiagen.com)

A frozen section should not be used for an "on-the-spot" diagnosis, since frozen tissue rendered tissue morphology is less optimal for microscopic examination. (medscape.com)

The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. (wikipedia.org)

Alternatively, vibratome sections do not require the tissue to be processed through organic solvents or high heat, which can destroy the antigenicity, or disrupted by freeze thawing. (wikipedia.org)
The disadvantage of vibratome sections is that the sectioning process is slow and difficult with soft and poorly fixed tissues, and that chatter marks or vibratome lines are often apparent in the sections. (wikipedia.org)
Safely deliver consistent, quality frozen sections - fast. (leicabiosystems.com)
Tissue sections were evaluated on a Leica DMLB microscope. (cdc.gov)

Acute stretch-shortening cycle contractions effects on skeletal muscle morphology in young and old rats. (cdc.gov)

Optimal tissue embedding is essential for smooth sectioning and ribboning. (leicabiosystems.com)

PAXgene Tissue FIX Containers (50 ml) are single-chamber containers prefilled with 50 ml of PAXgene Tissue FIX. (qiagen.com)

The formation of syncytium is accompanied by profound transcriptional changes and active metabolism in infected tissues. (biomedcentral.com)

Samples were directionally frozen in a sandwich format either in 10% DMSO or culture medium with the sample movement at 30 μm/s through the predetermined temperature gradients along a 2.6 mm slit. (huji.ac.il)

Many showed uniform plaque morphology and grew to high titre in tissue culture. (who.int)
The presence of CRP, complement, and oxLDL in a high proportion of plaque tissue from patients with unstable coronary artery disease implies that these surrogate markers have important proinflammatory effects inside atherosclerotic plaques. (bmj.com)

We are preferably using transparent indium-tin oxide (ITO) coated glass slides which allow us to perform morphological assessment of the analyzed tissue after MSI, e.g. by optical microscopy. (theratype.com)

OBJECTIVE: To investigate the microhardness distribution throughout the human hand phalanges using the Vickers method, which can be used to directly evaluate the bone mechanical properties at tissue level and provide an alternative means to investigate bone quality. (bvsalud.org)

Derivation, expansion, and freezing of fibroblasts from freshly collected skin biopsy. (lu.se)

and when about 50C place the tissue in the agarose, in a bigger mould. (utsouthwestern.edu)
melted, cooling agarose into a mould , place the tissue and cool quickly. (utsouthwestern.edu)

Tissue force and stress were unaltered by storage and preconditioning compared to fresh samples, while high preconditioning increased the stiffness and modulus (p ≤ 0.007). (lu.se)
The present collection includes primary material (scabs), frozen liquid cultures and lyophilized samples. (who.int)
Tissue microarrays permit a simultaneous and comparative analysis of tissue samples from hundreds to thousands of patients. (theratype.com)
Stool samples from an outbreak that are submitted to CDC for detection of viral pathology should be collected in bulk from 10 ill persons during their first 48 hours of illness, while feces are still liquid, and should be stored at 4 C (not frozen). (cdc.gov)

the other half was held fresh at 4°C. Upon receipt, we collected small pieces of fresh and formalin-fi xed tissue from several locations in the cerebrum and cerebellum and along the brain stem and cervical spinal cord. (cdc.gov)
fresh-frozen right limbs were used. (bvsalud.org)

The dermis offers low resistance, as do almost all internal tissues except bone, which is a poor conductor of electricity. (medscape.com)

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