Morphology in human epidermal keratinocytes human skin cell. Medical search. Frequent questions

Hence, in addition to the epidermal layer, the dermal layer will also be damaged. (wikipedia.org)
Following wounding, tissues were fixed and immunostained with markers of epidermal differentiation as well as a marker of fibroblasts allowing simultaneous visualization of migrating keratinocytes (keratin 14), differentiated suprabasal cells (involucrin), and dermal fibroblasts (vimentin) within the wound. (mattek.com)
Relative to Bowen disease, actinic keratosis (AK) only occurs on sun-damaged skin, is usually smaller, and retains a squamous differentiation toward the surface, often even when dermal invasion is developing. (medscape.com)
First, we cocultured dermal fibroblasts with dermal microvascular endothelial cells at a ratio of 2 : 1. (hindawi.com)
More recent skin substitutes combine epidermal and dermal layers by introducing fibroblasts and keratinocytes into an acellular matrix [ 5 ]. (hindawi.com)
The steady deterioration of the dermal and epidermal layers of the skin with age is caused by a combination of factors including reduced cellular proliferation and collagen synthesis, extracellular matrix remodeling, and changes in epidermal morphology (Crane 2015). (ernolaszlo.com)
Dermal macrophages dispose of viruses and bacteria that penetrate the chemical and physical barriers of the skin and also act as antigen presenters (Marieb 2019). (ernolaszlo.com)
In dermal fibroblasts culture in vitro, the mixed scaffold induced higher cell seeding efficiency and improved fibroblast adhesion and proliferation compared with collagen sponge. (nanofiberlabs.com)
In this work, keratinocytes and fibroblasts were cultured as epidermal equivalent and dermal equivalent respectively. (nanofiberlabs.com)
FBN2 silencing recapitulates hypoxic conditions and induces elastic fiber impairment in human dermal fibroblasts. (mcgill.ca)
Antioxidant, proliferation and migration assay of GSE was performed with human dermal papilla cells (hDPCs). (biomedcentral.com)
GSE treatment proliferated and migrated human dermal papilla cells (hDPCs) more than treatment of 10 μM minoxidil. (biomedcentral.com)
All 5 criteria listed above are needed for a diagnosis of type 1 bullous systemic lupus erythematosus, whereas only the first 4 criteria are needed to diagnose type 2 (undetermined location of antigen or dermal antigen other than type VII collagen) and type 3 (epidermal antigen) bullous systemic lupus erythematosus. (medscape.com)
Histopathological study revealed a reduction in inflammatory infiltrate and the presence of multiple hair follicles on day 7 after treatment with ASCs, promoting epidermal and dermal regeneration. (biomedcentral.com)
However, dermal chemical exposure may impact reactions in the skin differently depending on the strain of mouse. (cdc.gov)

UV exposure would also lead to the activation of receptors for epidermal growth factor, IL-1, and TNF-α in keratinocytes and fibroblasts, which then activates signaling kinases throughout the skin via an unknown mechanism. (wikipedia.org)
To establish suitable conditions for incorporating macrophages into skin models, we evaluated the effects of different media on primary keratinocytes, fibroblasts and macrophages. (cellntec.com)
A fibrous sheet was formed by the interactions between the fibroblasts and the endothelial cells, and capillary-like structures were observed after 20 days of coculture. (hindawi.com)
For example, seeding fibroblasts or keratinocytes results in faster vascularization due to growth factor secretion. (hindawi.com)
Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. (nature.com)

In this process, the keratinocytes progress from the basal layer of the epidermis through distinct stages to form the spinous, granular and cornified layers. (medicaljournals.se)
In addition to the low expression in normal skin and the high expression in the psoriatic epidermis, psoriasin is also expressed in conditions displaying abnormal differentiation. (medicaljournals.se)
In addition, the gene that encodes psoriasin is positioned within the epidermal differentiation complex (EDC) (10-12), a gene cluster that contains several genes of importance in the terminal differentiation of the human epidermis (10, 11). (medicaljournals.se)
The high expression level in psoriatic epidermis in conjunction with the disturbed differentiation process suggests that psoriasin is involved in keratinocyte differentiation. (medicaljournals.se)
[ 12 ] An underrecognized role for UV-A was also previously described that localizes the corresponding fingerprint mutations in the basal epidermis harboring the vital stem cells, as opposed to the suprabasal UV-B-induced mutations. (medscape.com)
HE staining showed that tissue-engineered skin exhibited a stratified epidermis after 7 days. (hindawi.com)
1: Epidermal dysplasia is characterized by the development of a zone in the epidermis showing defects in morphology, differentiation or organization. (cea.fr)
This test uses the EpiDerm™ reconstructed human epidermis model (MatTek) which consists of normal human-derived epidermal keratinocytes (NHEK) and therefore represents in vitro the target organ of the species of interest and closely resembles the biochemical and physiological properties of the upper parts of the human skin, i.e. the epidermis. (europa.eu)
Psoriasis is characterized by keratinocyte proliferation and immune cell accumulation in epidermis/dermis [ 1 ]. (thno.org)
Excessive proliferation of keratinocytes, abnormalities in the differentiation process, and continuous shedding of the thickened epidermis are characteristics of psoriasis (Figures 1 (a) and 1 (b)) [ 2 ]. (hindawi.com)
b) Epidermal regular psoriasiform hyperplasia in the epidermis and parakeratosis with Munro's microabscesses. (hindawi.com)
Assessment of the human epidermis model SkinEthic RHE for in vitro skin corrosion testing of chemicals according to new OECD TG 431. (episkin.com)
Reconstructed epidermis versus human and animal skin in skin absorption studies. (episkin.com)
In this study, the impact of triclosan exposure on the skin barrier and keratinocyte function was investigated using a model of reconstructed human epidermis. (cdc.gov)
The apical surface of reconstructed human epidermis was exposed to triclosan (0.05-0.2%) once for 6, 24, or 48h or daily for 5 consecutive days. (cdc.gov)

We have shown previously that extracellular psoriasin binds to the receptor for advanced glycation end products (RAGE), promoting endothelial cell proliferation (8). (medicaljournals.se)
Sustained hypoxia or lack of nutrients leads to fibroblast dysfunction, decreased keratinocyte migration and proliferation, and tissue loss [ 6 ]. (hindawi.com)
The low pH of the skin-referred to as the acid mantle-inhibits the proliferation of bacteria. (ernolaszlo.com)
Although steady-state conditions revealed no increase in primitive cell proliferation in p21-null mice, a significantly larger fraction of quiescent neural precursors was activated in the hippocampus and subventricular zone after brain ischemia. (rupress.org)
Therefore, p21 is an intrinsic suppressor to neural regeneration after brain injury and may serve as a common molecular regulator restricting proliferation among stem cell pools from distinct tissue types. (rupress.org)
Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
The final evaluation experiment results showed that the collagen in the middle layer significantly improved the adhesion and proliferation of SMC, and the proliferation of endothelial cells increased significantly with culture, indicating the non cytotoxicity of the construct. (nanofiberlabs.com)
The final results showed that the cell proliferation in the double-layer scaffold was higher than that in the single-layer CP nano pad and PLLA microdisk. (nanofiberlabs.com)

Wound healing is a fundamental process to re-establish tissue integrity and skin barrier function. (mattek.com)
Advances in tissue engineering technology have led to the production of novel human skin equivalents and organoids that reproduce cell-cell interactions with tissue-scale tensional homeostasis, and enable us to evaluate skin tissue morphology, functionality, drug response and wound healing. (mdpi.com)
Angiogenesis is an important factor for tissue-engineered skin constructs. (hindawi.com)
In this study, we constructed a scaffold-free bilayered tissue-engineered skin containing a capillary network. (hindawi.com)
Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. (hindawi.com)
Skin tissue substitutes must readily adhere, have good physical and mechanical properties, and be nonantigenic [ 2 ]. (hindawi.com)
Thus, the long-term survival and function of 3D tissues depend on the rapid development of new blood vessels to provide nutrients and oxygen to cells in the center of the tissue grafts. (hindawi.com)
The long-term goal of Dr. Bigliardi-Qi's neuro-immuno interface lab is to understand the molecular mechanisms underlying epithelial tissue regeneration, differentiation and immune function, and skin keratinocyte involvement in skin sensory, tissue repair, and immunological disorder. (umn.edu)
The peripheral tissue clock is exposed to light and external environmental factors, making it a very important aspect of skin disorders. (umn.edu)
Therefore, many laboratories try with great effort to develop resorbable tissue scaffolds that could support the patient´s cells. (nature.com)
ECM provides mechanical support for cells and also determines the shape of tissue 9 . (nature.com)
The cyclin-dependent kinase inhibitor, p21 cip1/waf1 (p21), maintains hematopoietic stem cell quiescence, and we evaluated its role in the regenerative response of neural tissue after ischemic injury using the mice deficient in p21. (rupress.org)
The nanofiber scaffold produced by the invention can be used for specific tissue engineering applications, including bone, cartilage, blood vessel and skin tissue regeneration. (nanofiberlabs.com)
The research themes rank around molecular mechanisms matrix proteins and fibers exert in disorders affecting blood vessels, bone, skin and adipose tissue. (mcgill.ca)
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
Multiple studies have made use of RGD hydrogel in different tissue and cell types. (thewellbio.com)
H-E staining showed tumor tissue-like structures specific to each cell line. (medsci.org)
These characteristics make MSCs interesting tools for tissue engineering and regeneration in human clinical trials. (biomedcentral.com)
Intradermal injection of lentiviral vectors corrects regenerated human dystrophic epidermolysis bullosa skin tissue in vivo. (medscape.com)

Histological and immunohistochemical analysis showed keratinocyte migration at 2 days following wounding. (mattek.com)

Vesiculobullous skin lesions can also develop as a result of extensive damage to the epidermal basal layer (and even suprabasal keratinocytes), due to an intense interface dermatitis in the setting of lupus erythematosus-specific skin disease. (medscape.com)

The development of dysplastic zones was observed, particularly in the basal layer, in a manner similar to the dysplasia¹ observed in early-phase skin carcinoma. (cea.fr)
The basal layer of human skin contains melanocytes , specialized pigment cells that harbor subcellular organelles called melanosomes in which melanin pigments are synthesized and stored and then transferred to keratinocytes (2). (mixedracestudies.org)

ELISA demonstrated that vascularization was promoted by significant upregulation of vascularization associated growth factors due to interactions among the 3 types of cells in the bilayer, as compared to cocultures of fibroblast and endothelial cells and monocultures. (hindawi.com)
Increasing evidence suggests there is a 2-way relationship between stress and skin quality, with direct impacts on keratinocyte and fibroblast number and function as well as on the composition of the extracellular matrix. (ernolaszlo.com)
Fibroblast cell therapy enhances initial healing in recessive dystrophic epidermolysis bullosa wounds: results of a randomized, vehicle-controlled trial. (medscape.com)

In contrast, wounded tissues cultured with growth factors demonstrated a dramatic increase in healing rate as keratinocyte migration completely covered the wounded area by day 6. (mattek.com)
The targeted nanohybrids also suppressed neutrophil migration by reducing CXCL1 and CXCL2 release from keratinocytes. (thno.org)
The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. (nature.com)
these cells are able to secrete soluble factors that stimulate the migration, mitosis, and differentiation of local stem cells. (biomedcentral.com)

Psoriasin (S100A7) was first identified for its overexpression in psoriatic keratinocytes (3), where it was found to be located to the nucleus and the cytoplasm (4). (medicaljournals.se)
Previous reports have shown that VEGF overexpression in keratinocytes augments wound vascularization [ 13 ]. (hindawi.com)
AN2728-loaded antibody-conjugated nanocarriers inhibited cytokine/chemokine overexpression in activated keratinocytes without affecting cell viability. (thno.org)
The overexpression of a single transcription factor in MSCs may promote transdifferentiation into a specific cell lineage, which could then be used for the treatment of some diseases. (biomedcentral.com)

Psoriasin, which is highly expressed in psoriasis, is encoded by a gene located within the epidermal differentiation complex. (medicaljournals.se)
The aim of this study was to investigate the effect of endogenous psoriasin on disturbed keratinocyte differentiation in psoriasis. (medicaljournals.se)
In psoriasis, the differentiation process of the epidermal cells is disturbed. (medicaljournals.se)
Previous studies of the role of psoriasin in keratinocyte differentiation have focused on the effects of extracellular psoriasin, but as the psoriasin in psoriasis is keratinocyte-derived, it is imperative to consider the effects of psoriasin as an endogenously produced intracellular mediator. (medicaljournals.se)
In a psoriasis-like skin mouse model, the actively targeted nanoparticles were superior to free drug and non-targeted nanoparticles in mitigating skin inflammation. (thno.org)
Intervention with the targeted nanosystem reduced the epidermal thickness of the psoriasiform lesion from 191 to 42 µm, decreased the Psoriasis Area Severity Index by 74%, restored barrier function, and returned chemokine levels to baseline. (thno.org)
Psoriasis is an autoimmune skin inflammation involving interactions between keratinocytes and immune cells. (thno.org)
Generalized pustular psoriasis (GPP) is a rare, chronic, and severe inflammatory skin disorder characterized by sudden eruption of sterile pustules, often accompanied by systemic inflammation. (springer.com)
Skin manifestations of psoriasis include five forms or phenotypes: plaque psoriasis or psoriasis vulgaris, inverse, pustular, guttate, and erythrodermic psoriasis. (springer.com)
The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
Our findings provide the molecular mechanisms by which Tan IIA can be used to treat psoriasis and support the traditional use of Salvia miltiorrhiza Bungee (Labiatae) for psoriasis and related skin diseases. (hindawi.com)
Psoriasis is a chronic inflammatory skin disease affecting 0.6% to 4.8% of people worldwide [ 1 ]. (hindawi.com)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
Aquaporin 3 (AQP3) plays an important role in the expression of various skin-related diseases such as vitiligo and psoriasis. (e-ajbc.org)

Exposure to triclosan increased epidermal permeability and altered the expression of genes involved in formation of the skin barrier. (cdc.gov)

The virus induced skin lesions histologically characterized by leukocyte infiltration and eosinophilic cytoplasmic inclusions. (cdc.gov)
With constant exposure to UVB rays, signs of photoaging might appear and precancerous lesions or skin cancer may develop. (wikipedia.org)
The keratinocytes in active psoriatic lesions display an altered sequence and altered expression levels of the differentiation markers (2). (medicaljournals.se)
Epidermal nevi are congenital lesions that affect about 1 in 1,000 people. (beds.ac.uk)

Use of PriCoat TM T25 Flasks or Applied Cell Extracellular Matrix is required for cell adhesion to the culture vessels. (topsan.org)
The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. (nature.com)
Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. (nature.com)
In epithelial cells, apical-basal polarity is maintained through the formation of several intercellular adhesion systems consisting of adherens junctions (AJs), desmosomes, and tight junctions (TJs). (rupress.org)
Good for adhesion cells or cells requiring stronger cell-matrix interactions. (thewellbio.com)
Each cell line formed spheroidal cell aggregates and showed enhancement of cell adhesion molecules over time. (medsci.org)
Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. (medsci.org)

Cutaneous squamous cell carcinoma (SCC) is an invasive malignant neoplasm of epidermal keratinocytes showing squamous phenotypic differentiation (see the following image). (medscape.com)
The majority (70%) of cutaneous squamous cell carcinoma (SCC) occurs on the head and neck, with an additional 15% found on the upper extremities. (medscape.com)
In lymphomas I have conducted studies in rare aggressive primary cutaneous tumors such as gamma/delta T cell lymphoma (expanding the description of the epidermotropic variant) as well as more indolent entities such as lymphomatoid papulosis (type D) and CD4-positive small to medium size T cell lymphoproliferative disorders. (mdanderson.org)
Such patients may present with a severe form of acute or subacute cutaneous lupus erythematosus (SCLE) that resembles erythema multiforme (Rowell syndrome)[4] or toxic epidermal necrolysis (TEN). (medscape.com)

citation needed] Additionally, UV radiation would cause the down-regulation of an angiogenesis inhibitor, thrombospondin-1, and the up-regulation of an angiogenesis activator which is platelet-derived endothelial cell growth factor, in keratinocytes. (wikipedia.org)
Pulmonary arterial hypertension (PAH) is a progressive disease characterized by lung endothelial cell dysfunction and vascular remodeling. (surrey.ac.uk)
Induced EndoMT cells exhibited up-regulation of mesenchymal markers, including collagen type I and α-smooth muscle actin, and a reduction in endothelial cell and junctional proteins, including von Willebrand factor, CD31, occludin, and vascular endothelial-cadherin. (surrey.ac.uk)

The dynamics of cell-extracellular matrix interactions in normal and pathological situations. (mcgill.ca)
The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. (mcgill.ca)
We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)

A model of wound healing was created by introducing epidermal wounds in a full thickness in vitro human skin model (EpiDermFT) using a 3-mm punch biopsy and subsequently evaluated at multiple recovery time points. (mattek.com)
Bowen disease (BD) is a squamous cell carcinoma in situ (SSCIS) with full-epidermal thickness dysplasia that has the potential for significant lateral spread before invasion. (medscape.com)

Dermatophytes are fungal organisms which infect keratin of humans and animals. (thedoctorsdoctor.com)

Novel in-vivo human studies have been conducted using photoacoustic and/or in-vivo Raman confocal microscopy. (umn.edu)
Flow cytometry and confocal microscopy showed a 9-fold increase in keratinocyte uptake of targeted nanohybrids relative to non-targeted nanoparticles. (thno.org)

[ 9 ] Gewirtzman et al reported a case of HPV-induced SCC in patients with epidermal dysplasia verruciformis. (medscape.com)
Ectodermal dysplasia-skin fragility syndrome resulting from a new homozygous mutation, 888delC, in the desmosomal protein plakophilin 1. (medscape.com)

Factors such as viruses (human papillomavirus [HPV]-5, -8, -11, -16, -18, and -38) and chemical agents (arsenic, tar, etc) act as co-carcinogens in aggravating an underlying element of vulnerability to malignant transformation. (medscape.com)

Intracellular psoriasin has been found to interact with various signalling proteins, including the nuclear factor (NF)-kB regulator Jab1, while secreted psoriasin functions as an antimicrobial peptide and has the ability to induce chemotaxis of neutrophils and T cells (6). (medicaljournals.se)

VitroGel ® RGD High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. (thewellbio.com)

Based on a better understanding of the basement membrane zone (BMZ) and the genes responsible for its components, newer treatments (eg, gene or protein therapy) may provide solutions to the skin fragility found in patients with epidermolysis bullosa. (medscape.com)
ASC treatment upregulated granulocyte-macrophage colony stimulating factor (GM-CSF) at the gene level, which may contribute to the recruitment of cells that participate in skin repair to the site of injury. (biomedcentral.com)
Corrective gene transfer of keratinocytes from patients with junctional epidermolysis bullosa restores assembly of hemidesmosomes in reconstructed epithelia. (medscape.com)
Gene expression changes were observed with PPAR isoforms in the liver and skin along with changes in genes involved in steatosis, fatty acid metabolism, necrosis, and inflammation. (cdc.gov)

2 Erythema multiforme, which appears 1-10 days after recurrent herpes, is the result of cell-mediated immunity (CMI) against viral antigen-positive cells. (webmedcentral.co.uk)

Using opioid receptor knockout mice and human chronic pruritus trials, her lab has demonstrated that chronic itch is not histamine-mediated, but rather a neuronal and immune disorder. (umn.edu)
Continued progression of cellular degradation results in collective stripping of the myelin sheaths of the cells, loss of neuronal conductance, and, subsequently, numbness. (ceufast.com)

Visible damage on test system: The test item did not show significantly reduced cell viability in comparison to the negative control after 4 hours of exposure. (europa.eu)
Skin maturation was affected by culture in macrophage medium, while macrophages showed reduced viability, altered cell morphology and decreased response to pro- and anti-inflammatory stimuli in skin differentiation media, both in 2D and 3D. (cellntec.com)

The replacement of animal models for investigation of inflammation and wound healing has been advancing by means of in vitro skin equivalents with increasing levels of complexity. (cellntec.com)

In a study published in Cells , researchers from LGRK (IRCM/CEA-Jacob) showed that human skin stem cells are sensitive to low doses of genotoxic stress in the setting of active regeneration. (cea.fr)
Researchers at the Genomics and Radiobiology of Keratinopoiesis Laboratory (LGRK) took a closer look at the impact of this source of stress on human skin stem cells. (cea.fr)
In skin biology, these research models are used to study, for example, skin barrier disorders in atopic dermatitis, genetic skin diseases, or the regenerative potential of skin stem cells. (cea.fr)
Fig. 3 Small Aperture (125-250 μ m) In vitro cartilage differentiation of human bone marrow mesenchymal stem cells induced by β - lactamase scaffold Large specific aperture (425-600 μ m) The stents in our body better support cartilage formation. (nanofiberlabs.com)
Adult mesenchymal stem cells (MSCs) have been widely used in regenerative medicine in both in vitro and in vivo preclinical research and clinical trials. (biomedcentral.com)
In fact, adipose mesenchymal stem cells (ASCs) are being used, especially in small breeds where the amount of bone marrow that can be harvested is limited and the collection is more laborious and painful. (biomedcentral.com)
Correction of junctional epidermolysis bullosa by transplantation of genetically modified epidermal stem cells. (medscape.com)

It is also clear that psychological stressors lead to aberrant barrier function, with decreased epidermal lipid and structural protein synthesis, decreased hydration of the stratum corneum, and increased transepidermal water loss (Maarouf 2019), and can also result in immune system dysfunction leading to systemic and local inflammation (Liu 2017). (ernolaszlo.com)

The histological morphology of hair follicles demonstrated an active anagen phase with the induction of stem cell factor. (biomedcentral.com)

In a spectrum of conditions of dysregulated keratinocyte differentiation, we have previously demonstrated strong psoriasin expression in differentiated squamous cell carcinoma and a lack of expression in undifferentiated basalioma (9). (medicaljournals.se)
This image shows a central keratotic squamous cell carcinoma with a raised, undermined periphery in a background of severely sun-damaged skin. (medscape.com)
See Squamous Cell Carcinoma , Bowen Disease , and Actinic Keratosis for more information. (medscape.com)
[ 3 ] occupational exposure, medical treatment (psoralen + ultraviolet A [PUVA]), or tanning beds is principally responsible for the development of squamous cell carcinoma (SCC). (medscape.com)
хвороба Боуена Bowen disease is a superficial squamous cell carcinoma in situ. (msdmanuals.com)

EpiDermFT exhibits stratified epidermal components and a fully developed basement membrane resembling in vivo skin in regard to both morphology and barrier function. (mattek.com)
For their study published in Cells , the LGRK researchers created human skin organoids from skin stem and progenitor cells and transplanted them to immunodeficient mice to reproduce a context of in vivo skin regeneration. (cea.fr)
Neural precursor cells from adults have exceptional proliferative and differentiative capability in vitro yet respond minimally to in vivo brain injury due to constraining mechanisms that are poorly defined. (rupress.org)
We assessed whether cell cycle inhibitors that restrict stem cell populations in other tissues may participate in limiting neural stem cell reactivity in vivo. (rupress.org)
Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
We determined the presence of EndoMT in the pulmonary vasculature in vivo and the functional effects on pulmonary artery endothelial cells (PAECs) undergoing EndoMT in vitro. (surrey.ac.uk)

MCAP [megalencephaly-capillary malformation] syndrome and CLOVES [congenital lipomatous asymmetric overgrowth of the trunk, lymphatic, capillary, venous, and combined-type vascular malformations, epidermal nevi, skeletal and spinal anomalies] syndrome). (beds.ac.uk)

Skin-resident dendritic cells act as a first line of defense, ingesting foreign substances, processing them, and presenting them to lymphocytes to activate the immune system. (ernolaszlo.com)
DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
BALB/c mice exposed to triclosan (2%) had a higher number and frequency of neutrophils and lower number and frequency of dendritic cells in the skin compared to controls. (cdc.gov)

The dermis is the second major layer of the skin and it comprises collagen, elastin, and extrafibrillar matrix which provides structural support to the skin. (wikipedia.org)
The up-regulation of MMP can occur even after minimal exposure to UV, hence, exposure to UV radiation which is inadequate to cause sunburn can thus facilitate the degradation of skin collagen and lead to presumably, eventual photoaging. (wikipedia.org)
Thus, collagen production is reduced in photoaged skin due to the process of constant degradation of collagen mediated by MMPs. (wikipedia.org)
Most of these methods involve culturing endothelial cells on or within ECM substrates (e.g., collagen, fibrin, fibronectin, laminin, etc.) or other types of biomaterials to form capillary-like structures [ 15 ]. (hindawi.com)
However, these methods have some drawbacks because the ECM substitutes contained exogenous materials such as bovine collagen, dead human allogenic dermis, or synthetic polymers [ 16 ]. (hindawi.com)
In most cases, PLLA, collagen and gelatin are often combined to produce nanofiber scaffolds, which show physical and biological characteristics matching with skin substitutes. (nanofiberlabs.com)
Injection of recombinant human type VII collagen restores collagen function in dystrophic epidermolysis bullosa. (medscape.com)

Aging is a complex, progressive process that leads to functional and aesthetic changes in the skin. (wikipedia.org)
Moreover, skin substitutes should restore functional anatomy and physiology after treatment and healing of the wound [ 4 ]. (hindawi.com)
Additionally, different microscopy and immuno- histochemistry are used for morphology and spatiotemporal biomarkers, and functional analysis. (umn.edu)
We sought to identify PKA phosphorylation sites in PLIN5 and assess their functional relevance in cultured cells and the livers of mice. (uci.edu)

Later studies have demonstrated secretion of psoriasin from epithelial cells (5). (medicaljournals.se)
Her lab discovered various taste and olfactory receptors in skin epithelial cells and demonstrated that they act as important chemical sensors. (umn.edu)
Our data indicate that Tiam1-mediated activation of Rac in PAs controls TJ biogenesis and polarity in epithelial cells by association with and activation of the Par3-Par6-aPKC polarity complex. (rupress.org)

The Langerhans cells may undergo changes in quantity, morphology, and function due to UV exposure and may eventually become depleted. (wikipedia.org)
To establish the skin barrier and the epidermal structure, keratinocytes undergo a tightly regulated process of differentiation. (medicaljournals.se)
MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)

We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)

However, the current in vitro skin models still have a limited pre-clinical relevance due to their lack of immune cells. (cellntec.com)
So far, few steps have been made towards the incorporation of immune cells into in vitro skin and the requirements for immunocompetent co-cultures remain unexplored. (cellntec.com)
these responses may include proinflammatory cytokines, inflammasome activation, barrier integrity, skin microbiota, and the presence of immune cells. (cdc.gov)

In both methods, wounded tissues cultured without growth factors (2% human serum) had a reduced healing rate in which keratinocytes did not cover the entire wound within a 6-day timeframe. (mattek.com)
The 3D skin models her group developed included advanced melanoma and standardized wound models. (umn.edu)
In addition, the evaluation of genes and proteins showed that the wound healing was active, which again confirmed that the double-layer scaffold could provide a suitable microenvironment for stimulating skin regeneration. (nanofiberlabs.com)
In this paper, we evaluated the clinical value and safety of the application of cultured allogenic MSCs from AT for acute and chronic skin wound healing in a canine model. (biomedcentral.com)
The development of an allogenic ASC therapy to improve wound healing in a canine model could have a clinical impact in human treatment. (biomedcentral.com)

FAM20C loss-of-function mutations cause Raine syndrome in humans, characterized by generalized osteosclerosis, distinctive craniofacial dysmorphism, along with extensive intracranial calcification. (bvsalud.org)
Epidermolysis bullosa is classified into four major categories: (1) epidermolysis bullosa simplex (intraepidermal skin separation), (2) junctional epidermolysis bullosa (skin separation in lamina lucida or central BMZ), (3) dystrophic epidermolysis bullosa (sublamina densa BMZ separation, as in the images below), and (4) Kindler syndrome (extremely rare, blistering at any level). (medscape.com)

Similar to wild-type (WT) keratinocytes, Tiam1-deficient cells establish primordial E-cadherin-based adhesions, but subsequent junction maturation and membrane sealing are severely impaired. (rupress.org)
Tiam1 and V12Rac1 can rescue the TJ maturation defect in Tiam1-deficient cells, indicating that this defect is the result of impaired Tiam1-Rac signaling. (rupress.org)

Compromised skin terrain from underlying chronic conditions such as chronic ulcers, lichen sclerosus et atrophicus , bullous diseases, and some genodermatosis result in increased susceptibility to the development of SCC. (medscape.com)
Together, these factors interact to generate the visible and invisible signs of aging, make the skin more susceptible to environmental insult, and lower the threshold at which skin diseases manifest. (ernolaszlo.com)
This white paper will not consider patients with skin diseases associated with a primary psychiatric disorder. (ernolaszlo.com)
To a large extent, leprosy is largely considered one of the oldest diseases ever documented in human colonies. (ceufast.com)
Contagious ecthyma and milker's nodules are two viral skin diseases that are rarely transmitted from animals to humans. (msdmanuals.com)
8,9 Atopic dermatitis is the most disabling condition among skin diseases, with a lifetime prevalence of 10-20% in children and 1-3% in adults. (webmedcentral.co.uk)

Skin substitutes seeded with cells producing growth factor on a continuous basis provide an attractive alternative [ 12 ]. (hindawi.com)
The results showed that compared with pure PLLA nanofibers, the mineral growth, ALP activity and cell morphology of the modified structure were in the best state. (nanofiberlabs.com)
Induced EndoMT in PAECs by inflammatory cytokines IL-1β, tumor necrosis factor α, and transforming growth factor β led to actin cytoskeleton reorganization and the development of a mesenchymal morphology. (surrey.ac.uk)
GSE treatment significantly reduced the number of mast cells and the expression of transforming growth factor beta 1 in mouse skin tissues. (biomedcentral.com)
The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. (thewellbio.com)

This skin model can be expanded to other epithelial model cultures, e.g. oral, respiratory, or gut epithelium. (umn.edu)

Additionally, studies have shown that exposure to triclosan dermally activates the NLRP3 inflammasome and disrupts the skin barrier integrity in mice. (cdc.gov)

Engineering skin substitutes hold promise for advanced treatment of acute and chronic skin wounds [ 1 ]. (hindawi.com)

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)
Western blotting was used for protein expression analysis of the collected cell masses (spheroids), and H-E staining was used for structural observation. (medsci.org)
Here we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. (uci.edu)

Quantitative imaging studies showed that this low radiation dose caused regeneration alterations and disturbed epidermal organization. (cea.fr)
In actual preparation, electrospun PLLA scaffold will be prepared in combination with surface modification to guide cells to differentiate into bone lineage and achieve the best bone regeneration performance. (nanofiberlabs.com)

Ulcerated nodules and plaques were observed on the lips (A), thorax (B), and forelimb (C). Skin punch biopsy specimen (D) showing leukocyte infiltration and cytoplasmic inclusion. (cdc.gov)
Obtain a skin biopsy following a thorough history and physical examination. (medscape.com)

Variation in skin pigmentation is due to the type and quantity of melanins generated, melanosome size, and the manner in which keratinocytes sequester and degrade melanins (4). (mixedracestudies.org)
1994). A rare subgroup of epidermal nevi is clinically indistinguishable from other epidermal nevi, but displays histopathologic features typical of epidermolytic hyperkeratosis (see EHK, 113800), and patients with this type of epidermal nevi sometimes have offspring with generalized EHK (Paller et al. (beds.ac.uk)
Although these changes were not observed in SKH1 mice, SKH1 mice exposed to triclosan had a higher number and frequency of type 2 innate lymphoid cells in the skin. (cdc.gov)

Regulation of epidermal m-opiate receptor (MOPr) improves itching, which is a major problem for aged skin with age-related peripheral neuropathy. (umn.edu)
Together, these results suggest that exposure to triclosan impacts skin barrier integrity and function of human keratinocytes and suggests that these alterations may impact immune regulation. (cdc.gov)

Photoaging or photoageing (also known as "dermatoheliosis") is a term used for the characteristic changes to skin induced by chronic UVA and UVB exposure. (wikipedia.org)

Alterations in the barrier and immune regulatory functions of the skin have been demonstrated to increase the risk of sensitization and development of allergic disease. (cdc.gov)

Induction of keratinocyte differentiation caused concurrent expression of psoriasin and the differentiation marker involucrin. (medicaljournals.se)

Selection of specific mouse strains to evaluate skin effects, such as haired (BALB/c) or hairless (SKH1) mice, varies dependent on experimental design and needs of a study. (cdc.gov)
Taken together, these results demonstrate that exposure to an immunomodulatory chemical, triclosan, differentially impacts immune cell populations in the skin of haired and hairless mice. (cdc.gov)

The epidermal layer does not contain any blood vessels or nerve endings but melanocytes and basal cells are embedded in this layer. (wikipedia.org)
Upon exposure to UVB rays, melanocytes will produce melanin, a pigment that gives the skin its color tone. (wikipedia.org)
Melanosome morphology and content differs between melanocytes that synthesize mainly eumelanins (black-brown pigments) or pheomelanins (pigments which range from yellow to reddish-brown) (3). (mixedracestudies.org)

Anatomy16

Organisms3

Diseases12

Chemicals and Drugs34

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes29

Disciplines and Occupations1

Information Science2

Named Groups1

Health Care3

Dermal15

Fibroblasts6

EPIDERMIS15

Proliferation8

Tissue19

Showed keratinocyte migration1

Suprabasal1

Basal layer2

Fibroblast3

Migration4

Overexpression4

Psoriasis14

Permeability1

Lesions4

Adhesion7

Cutaneous4

Endothelial cell3

Extracellular Matrix3

Thickness2

Keratin1

Confocal microscopy2

Dysplasia2

Malignant1

Intracellular1

Interactions1

Gene4

Antigen1

Neuronal2

Viability2

Equivalents1

Stem cells7

Lipid1

Hair follicles1

Squamous cell carc5

Vivo6

Vascular1

Dendritic cells3

Collagen7

Functional4

Epithelial cells3

Undergo3

HaCaT1

Immune cells3

Wound5

Syndrome2

Maturation2

Diseases6

Growth5

Epithelium1

Activates1

Wounds1

Protein3

Regeneration2

Biopsy2

Type3

Regulation2

Chronic1

Alterations1

Induction1

Mice2

Melanocytes3