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  • mature oocytes
  • Recombinant tUCHL1 absorbed p34(cdc2), a component of MPF, in obviously larger quantities from mature oocytes than from immature oocytes, and p13(suc1) was isolated from tUCHL1 with a dependence on the ATP regeneration system, suggesting that still other functions may be involved in their association that require phosphorylation. (jove.com)
  • Embryos Developed
  • When 2-cell embryos derived from ZIP fresh and vitrified oocytes inseminated with 2 day-stored spermatozoa were transferred into recipient females, 47.9 and 15.0% of the embryos developed to term, respectively. (nii.ac.jp)
  • germ
  • Co-expression of cyp26a1 and the pluripotent germ cell stem cell marker pou5f1(oct4) in meiotically arrested oocytes was consistent with roles in mouse to promote germ cell survival and to prevent apoptosis, mechanisms that are central for tipping the sexual fate of gonads towards the female pathway in zebrafish. (jove.com)
  • Oct4
  • The resulting OLCs express various markers consistent with oocytes such as Oct4 , Vasa , Bmp15 , and Scp3 . (jove.com)
  • Spermatozoa
  • Oocytes and spermatozoa are terminally differentiated haploid cells which can no longer divide. (mdpi.com)
  • The storage conditions were optimized by storing the mouse epididymides at 4 C in mineral oil or in the mouse body for up to 4 days after death, and the retrieved spermatozoa were used to fertilize fresh oocytes. (nii.ac.jp)
  • Spermatozoa retrieved from epididymides stored in mineral oil were then used to fertilize fresh and vitrified oocytes with or without ZIP treatment. (nii.ac.jp)
  • Molecular
  • This fourth edition of The Mouse Manual - Manipulating the Mouse Embryo - appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. (cshlpress.com)
  • significantly
  • In oocytes from hibernation-interrupted toads, the p34(cdc2) protein level was significantly lower than in oocytes from toads in artificial hibernation, providing an explanation for the different quantities isolated by recombinant tUCHL1 pull-down and, more importantly, identifying a mechanism involved in the toad oocytes dependence on a low environmental temperature during winter hibernation. (jove.com)
  • stem cells
  • New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable direct injection of RNA/DNA constructs into zygotes to achieve gene targeting, require new explanations and protocols. (cshlpress.com)
  • Various cell types could be analyzed using this technique, including lymphocytes/leukocytes, stem cells, and tumor cells. (jove.com)
  • gene
  • Additionally, many of the techniques to overexpress or inhibit gene function in organisms such as Xenopus and zebrafish fail to sufficiently target critical maternal signaling pathways, such as Wnt signaling. (jove.com)
  • In Xenopus , manipulating gene function in cultured oocytes and subsequently fertilizing them can ameliorate these problems to some extent. (jove.com)
  • adult
  • a larger number of oocytes were recovered at interestrus/anestrus when only adult donors were considered (p=0.0075). (vin.com)
  • 1. Young cats presented a greater number of oocytes than adult cats. (vin.com)
  • This protocol describes a set of labeling techniques that can be used to gauge renal composition and test nephron functionality in the adult zebrafish kidney. (jove.com)
  • Thus, these methods are widely applicable to the future phenotypic characterization of adult zebrafish kidney injury paradigms, which include but are not limited to, nephrotoxicant exposure regimes or genetic methods of targeted cell death such as the nitroreductase mediated cell ablation technique. (jove.com)
  • experimental
  • Isolated follicles can be used for a variety of experimental techniques, including in vitro development assays, live imaging, mRNA expression analysis and western blot analysis of proteins. (jove.com)
  • functional
  • By using electron microscopy, immunochemistry and immunofluorescence techniques in combination with functional studies and proteomic approaches, we here demonstrate that calcium ionophore-induced AE results in the formation of unilamellar hybrid membrane vesicles containing a mixture of components originating from the two fused membranes. (jove.com)
  • define
  • this allows one to combine genetic or pharmacologic perturbations with in vitro development to define the effects of such manipulations on the processes occurring during specific periods of development. (jove.com)