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  • Localization
  • Detailed subcellular localization and liposome binding assay indicates different interface anchoring regions in the protein, and anionic lipid seems to influence the binding properties of the anchoring segments. (diva-portal.org)
  • TorsinA is partitioned between the ER and the contiguous perinuclear space of the nuclear envelope, and its localization is controlled in part by its association with LAP1 and LULL1, type II transmembrane proteins residing in the nuclear envelope and ER, respectively ( 12 , - 14 ). (pubmedcentralcanada.ca)
  • interacts
  • By careful unraveling of this collection of structures, researchers could gain a more complete understanding of how the enzyme functions, details about how GlpD interacts with the membrane, works to catalyze the enzymatic reaction, and links to cellular-energy production. (webwire.com)
  • enzyme
  • Strongly overexpression of a monotopic glycolipid-synthesizing enzyme could induce massive formation of "foreign" vesicles in the cytoplasm. (diva-portal.org)
  • Using X-ray crystallography, researchers at the University of Pittsburgh School of Medicine led by structural biologist Joanne I. Yeh, Ph.D., have become the first to decipher the three-dimensional structure of a membrane-bound enzyme that plays a crucial role in glycerol metabolism a discovery that could lead to important advances against obesity, diabetes and a potential host of other diseases. (webwire.com)
  • The protein structure Dr. Yeh s team solved is a large enzyme called Sn-glycerol-3-phosphate dehydrogenase known simply as GlpD found in abundance in the cell membranes of almost all organisms, including humans. (webwire.com)
  • As a result, it is technically challenging to produce enough highly purified and active protein to obtain clear, relevant information about the enzyme s atomic structure. (webwire.com)
  • As part of these challenging studies, the Pitt researchers used novel peptide-based detergents called peptergents that they developed in their lab to carefully separate GlpD from the cell membrane and keep it in an active form to ensure that their studies revealed a physiologically relevant enzyme structure. (webwire.com)
  • The team then used detergents to crystallize the enzyme and screened the protein crystals in Pitt s new state-of-the-art X-ray crystallography facility, directed by Dr. Yeh. (webwire.com)
  • fractions
  • Methods to characterize MPs are limited by the lack of extraction protocols and reagents that allow sufficient amounts of MPs to be obtained from various cell types without cross-contamination from other protein fractions. (thermofisher.com)
  • outer
  • Secretins form multimeric channels across the outer membrane of Gram-negative bacteria that mediate the import or export of substrates and/or extrusion of type IV pili. (elifesciences.org)
  • A crown-like structure outside the outer membrane capping the secretin was found not to be part of PilQ. (elifesciences.org)
  • In Gram-negative bacteria this machinery spans the entire periplasm and connects the inner (IM) and outer membranes (OM), mediating DNA binding on the cell surface and subsequent translocation through the periplasm into the cell. (elifesciences.org)
  • translocation
  • The OM-embedded C-terminal secretin domain likely provides an aperture for DNA and protein translocation through the OM, connecting the periplasm to the extracellular environment. (elifesciences.org)
  • Synechocystis
  • To elucidate this question, we studied the function of NdbC, one of the three NDH-2s in Synechocystis , by constructing a deletion mutant (Δ ndbC ) for a corresponding protein and submitting the mutant to physiological and biochemical characterization as well as to comprehensive proteomics analysis. (plantphysiol.org)
  • biochemical
  • Monotopic proteins represent a specialized group of membrane proteins in that they are engaged in biochemical events taking place at the membrane interface. (diva-portal.org)
  • By our domain-specific feature machine-learning approach, we show indirectly that the environment (extracellular, cytoplasm, or vesicle) is reflected in the biochemical properties of protein domains reaching into that environment. (pnas.org)
  • receptors
  • SURFY was used to predict a human surfaceome of 2,886 proteins with an accuracy of 93.5%, which shows excellent overlap with known cell-surface protein classes (i.e., receptors). (pnas.org)
  • Membrane proteins and receptors are the largest category of druggable targets studied in the pharmaceutical industry. (thermofisher.com)
  • organisms
  • Enveloped by this unusual membrane, these structures may be more resistant to the normal processes that degrade and destroy foreign materials in humans and other organisms. (elifesciences.org)
  • translocase
  • In the Tat system, which is structurally and mechanistically unrelated to the Sec translocase, proteins are translocated in a fully folded form ( 2 , 3 ). (pnas.org)
  • topological
  • As a training set, we used experimentally verified high-confidence cell-surface proteins from the Cell Surface Protein Atlas (CSPA) and trained a random forest classifier on 131 features per protein and, specifically, per topological domain. (pnas.org)
  • Structures
  • This study marks the highest resolution structure of a monotopic membrane protein that scientists have solved to date, and is one of only a handful of structures of this important class of membrane proteins that have been determined. (webwire.com)
  • Where available, membrane protein crystal structures facilitate elucidation of their functions and discovery of mechanisms of MP-drug molecule interactions. (thermofisher.com)
  • We are working on the structures of the membrane arm and of the intact complex itself with the aim of understanding the proton pumping mechanism. (cam.ac.uk)
  • Depth
  • Many persons getting protein powders do not know the things they are acquiring as they don't genuinely understand how to study a nutrition label, and that's why we're going to explain the nourishment label in depth so you're able to fully realize what can make this protein powder the top of its variety. (ampedpages.com)
  • coli
  • Here, we report the functional co‐reconstitution of the E. coli YidC protein with the SecYEG complex, yielding proteoliposomes that catalyze Sec‐dependent membrane protein insertion. (embopress.org)
  • structural
  • Substitution of Mn(III) for Fe(III) causes no structural perturbations in the protein. (rcsb.org)
  • The approach used here will be useful for structural analysis of other oligomeric proteins that weakly assemble in the membrane. (pnas.org)
  • distinct
  • Both the pharmacological inhibition profile and the identified cleavage site between two juxtaposed cysteine residues are distinct from those of presently known proteases, suggesting that a hitherto uncharacterized, membrane-associated protease accounts for TorsinA processing. (pubmedcentralcanada.ca)
  • Ideally this Rule A person Protein Powder review has made it abundantly distinct that This can be the purest, most effective protein powder around. (ampedpages.com)
  • Interactions
  • Interactions are determined by geometric criteria as described in K. Stierand, M. Rarey (2010), Drawing the PDB: Protein-ligand complexes in two dimensions, ACS Med. (rcsb.org)
  • New NMR Avenues to Characterize the Interactions of Extended N-glycans with Proteins: The Influenza Hemagglutinin Case. (natureindex.com)
  • proton
  • In contrast, NDH-2s are composed of single polypeptides with molecular masses around 50 kD, and they do not contribute to the generation of the proton gradient across the membrane ( Yagi, 1991 ). (plantphysiol.org)