Mononuclear cells peripheral blood or umbilical cord of healthy. Medical search. Frequent questions

Mononuclear cell (MNC) prepared from human umbilical cord blood (CB) are candidate therapeutics for treating hypoxic-ischemic (HI) brain injuries. (biorxiv.org)

We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. (hindawi.com)
Mesenchymal stem cells (MSCs) are multipotent stem/progenitor cells which can differentiate to several mesodermal derivatives and possess an ability to secrete factors involved in neovascularization and immunomodulation [ 1 , 2 ]. (hindawi.com)
However, recent studies have demonstrated that other angiogenic cells called endothelial progenitor cells (EPCs) also play an important role during the formation of new vessels through the process of vasculogenesis [ 7 - 9 ]. (hindawi.com)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
We hypothesize that blood-derived endothelial progenitor cells (EPCs) have the required proliferative and vasculogenic activity to create vascular networks in vivo. (ashpublications.org)
Vasilyev Stanislav, Kubeš Miroslav, Marková Eva, Belyaev Igor: DNA damage response in CD133 + stem/progenitor cells from umbilical cord blood: Low level of endogenous foci and high recruitment of 53BP1. (sav.sk)

Null cells are large granular lymphocytes that develop inside the bone marrow and attack pathogens and abnormal cells. (wikipedia.org)
MSCs were obtained from the bone marrow (BM) cells remaining in the bag and filter used in the transplantation of hematopoietic stem cells. (springer.com)
MSCs could be derived from a series of tissues including but not limited to umbilical cord, placenta, adipose tissue, bone marrow, gingiva, and dental pulp [5-12]. (researchsquare.com)
Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
These mobilization agents increase circulating leukocytes and stimulate the bone marrow to produce a large number of hematopoietic stem cells, which are mobilized into the bloodstream, allowing for large quantities of stem cells and peripheral blood mononuclear cells (PBMCs) to be collected from a single donor. (cgt.global)
We offer G-CSF, Plerixafor, and G-CSF plus Plerixafor options to mobilize the cells from the bone marrow. (cgt.global)
The human MSC derived from bone marrow, adipose tissue, and umbilical cord matrix are from different origins, but with comparable biological properties and function. (promocell.com)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
Altaner Čestmír, Altanerová Veronika, Cihová Marína, Hunáková Ľubica, Kaiserova Katarina, Klepanec Andrej, Vulev Ivan, Madaric Juraj: Characterization of mesenchymal stem cells of "no-options" patients with critical limb ischemia treated by autologous bone marrow mononuclear cells. (sav.sk)
Madaric Juraj, Klepanec Andrej, Mistrík Martin, Altaner Čestmír, Vulev Ivan: Intra-arterial Autologous Bone Marrow Cell Transplantation in a Patient with Upper-extremity Critical Limb Ischemia. (sav.sk)
Magnetically isolated naive and non-naive CD4 + T-cells were stimulated under Th17-polarizing proinflammatory cytokine conditions in presence and absence of bone marrow derived mesenchymal stromal cells (MSCs). (biomedcentral.com)
In the 1970s, Friedenstein and colleagues [1] first reported that locally applied culture-expanded populations of bone marrow stroma-derived fibroblastic cells remained at their injection sites under the kidney capsule, where an ectopic hematopoiesis was initiated. (stemcellslab.net)
Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (stemcellslab.net)
Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (stemcellslab.net)
This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (stemcellslab.net)
letters to the editor: bone marrow transplantation and leukemic transformation of transplanted cells]. (liverpool.ac.uk)

The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. (springer.com)
NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. (springer.com)
There is a growing body of evidence that multipotent mesenchymal stromal cells' (MSCs') remarkable therapeutic potential is attributed not only to their differentiation and regenerative capacity, but also to the paracrine effect, underlying their immunomodulatory properties. (biomedcentral.com)
There is evidence that 2D or 3D culturing conditions have an impact on the cells' secretome, and here we aimed to compare the secretion of cytokines and growth factors in human MSCs from different sources cultured in 2D and 3D conditions and assess their effect on human macrophages polarization in vitro. (biomedcentral.com)
Human peripheral blood mononuclear cells-derived macrophages were then treated with umbilical cord-derived MSCs' conditioned media and their effect on macrophages polarization was assessed. (biomedcentral.com)
Our findings suggest that umbilical cord-derived MSCs' conditioned media demonstrated the highest cytokine and growth factor levels and despite mostly pro-inflammatory cytokine profile were able to promote anti-inflammatory macrophage polarization. (biomedcentral.com)
Umbilical cord-derived MSCs' conditioned media hold great potential for therapeutic use, demonstrating significant anti-inflammatory effect on human macrophages. (biomedcentral.com)
Multipotent mesenchymal stromal cells (MSCs) are an extensively researched yet still understudied tool for treating a wide range of pathological conditions, one particular area of research interest being their immunomodulatory properties. (biomedcentral.com)
MSCs are reported to contribute both to the inflammation initiation and to its resolution via complex cross talk with immune cells, most importantly macrophages [ 1 ]. (biomedcentral.com)
however, relatively low cell yield and high invasiveness of the harvesting procedure [ 9 ] have urged the quest for alternative MSCs sources. (biomedcentral.com)
Neonatal tissues, such as umbilical cord and placenta, have gained particular interest, giving a chance to harvest MSCs in a noninvasive manner. (biomedcentral.com)
The HUCMSCs derived different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stem cell therapy. (researchsquare.com)
Mesenchymal stem cells (MSCs) have been widely tested for treating a variety of refectory medical indications such as type 1 diabetes mellitus, systemic lupus erythematosus, rheumatoid arthritis and Crohn's disease due to their multiple differentiation potentials and immunomodulation capability [1-5]. (researchsquare.com)
Over the past few years, MSCs researches have achieved some inspiring results and some of which moved up to clinic period from preclinical phases, resulting in the marketing approval of a few cell-based therapy products (CTPs) by different national regulatory authorities [18]. (researchsquare.com)
We established a Good Manufacturing Practice (GMP) grade cell facility to produce clinic-grade human umbilical cord derived MSCs (HUCMSCs) for treating premature ovarian failure (POF) and recurrent uterine adhesion [18, 20]. (researchsquare.com)
Therapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. (hindawi.com)
Previous studies demonstrated that MSCs could ameliorate the pathology associated with ischemic heart disease, ischemic stroke, and peripheral vascular disease by releasing several critical factors that increase cell survival, provide an appropriate microenvironment for repairing damaged tissue, and induce neovascularization [ 3 - 5 ]. (hindawi.com)
However, recent studies showed that MSCs derived from distinct sources might produce distinct combination of factors that exert different effects on target cells [ 15 , 16 ]. (hindawi.com)
We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. (nature.com)
Our group recently reported that CD73 on the cell membrane also contributes to the immunomodulatory capacity of MSCs 3 , suggesting that MSCs exert their immunomodulatory properties in a multifactorial manner. (nature.com)
Platelet derivatives have been proposed as FBS substitutes for the ex-vivo expansion of mesenchymal stem/stromal cells (MSCs) since platelet-derived growth factors can promote MSC ex-vivo expansion. (biomedcentral.com)
Due to their immunomodulatory properties, mesenchymal stem cells (MSCs) have come into focus as a potential therapeutic concept. (biomedcentral.com)
The aim of this study was to investigate the influence of MSCs on the phenotype, cytokine profile, and functionality of naive and non-naive CD4 + T-cells from healthy donors (HD) and patients with autoimmune arthritis under Th17-cytokine polarizing conditions in an explorative way using a transwell system prohibiting any cell-cell-contact. (biomedcentral.com)
In isolated naive CD4 + T-cells from HD and patients, MSCs suppressed the differentiation of naive towards an effector phenotype while memory and naive cells showed higher percentages in culture with MSCs. (biomedcentral.com)
In patients, MSCs significantly decreased the proportion of IL-9 and IL-17 producing effector T-cells. (biomedcentral.com)
The results of the study indicate significant immunomodulatory properties of MSCs, as under Th17-polarizing conditions MSCs are still able to control T-cell differentiation and proinflammatory cytokine production in both HD and patients with autoimmune arthritis. (biomedcentral.com)
Mesenchymal stem cells (MSCs) are multipotent adult stem (or stromal) cells with the capacity of self-renewal and differentiation potential towards several mesodermal linages such as osteogenic, chondrogenic and adipogenic differentiation (more citations) [ 1 ]. (biomedcentral.com)
A shift from Th1 to Th2 cells demonstrated by an increase in the levels of IL-4 has been observed upon treatment with MSCs [ 8 ]. (biomedcentral.com)
Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (stemcellslab.net)
Therefore, the authors concluded that transplantation of isolated healthy allogeneic MSCs might cure the disease. (stemcellslab.net)
The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (stemcellslab.net)
and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (stemcellslab.net)

Kučerová Lucia, Školeková Svetlana, Matúšková Miroslava, Bohac Martin, Kozovská Zuzana: Altered features and increased chemosensitivity of human breast cancer cells mediated by adipose tissue-derived mesenchymal stromal cells. (sav.sk)

To explore these mechanisms further, we exposed mouse brain organotypic slice cultures to oxygen and glucose deprivation (OGD) and then treated the brain slices with cells from CB or adult peripheral blood (PB). (biorxiv.org)
To test this, EPCs isolated from human umbilical cord blood or from adult peripheral blood, and human saphenous vein smooth muscle cells (HSVSMCs) as a source of perivascular cells, were combined in Matrigel and implanted subcutaneously into immunodeficient mice. (ashpublications.org)
the colonies were similarly induced, but with a lower efficiency, in adult peripheral blood leukocyte cultures infected with the virus. (liverpool.ac.uk)

Null cells are present in small numbers in lymphoid organs but are often found in nonlymphoid tissues. (wikipedia.org)
Little is known about the maternal immune cells at this site, which are in direct contact with fetal tissues. (frontiersin.org)
Together, these findings indicate that term placental tissues attract MAIT cells, and that this effect is at least partly mediated by MIF. (frontiersin.org)
Vasculogenesis involves migration of EPCs from blood circulation into injured/ischemic tissues where they proliferate and generate new vessels de novo . (hindawi.com)
Placental tissues and umbilical cord blood were obtained from healthy newborns after receiving signed informed consents from their mothers. (hindawi.com)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
How do mesenchymal stem cells from different tissues differ in terms of their biological function? (promocell.com)

2016). Natural killer cell-based adoptive immunotherapy eradicates and drives differentiation of chemoresistant bladder cancer stem-like cells. (springer.com)
Human myelopoiesis is an exciting biological model for cellular differentiation since it represents a plastic process where multipotent stem cells gradually limit their differentiation potential, generating different precursor cells which finally evolve into distinct terminally differentiated cells. (biomedcentral.com)
1 One strategy is to seed the appropriate cells on a biodegradable scaffold engineered with the desired mechanical properties, followed by stimulation of cell growth and differentiation in vitro, such that, on implantation in vivo, the engineered construct undergoes remodeling and maturation into functional tissue. (ashpublications.org)
Transcriptome analysis revealed that Th1/Th2 differentiation, NK cells, Th17 cell, complement system and calcium signaling pathways were down-regulated post-treatment of Pam3CSK4. (bvsalud.org)
Depending on the tissue of origin, they may have a higher preference for differentiation into one particular cell type and a lower preference for another one, but they all still retain the differentiation potential for the mesenchymal lineage. (promocell.com)
Which plates does PromoCell recommend as 96-well U bottom suspension plates for chondrogenic differentiation of Human Mesenchymal Stem Cells? (promocell.com)
For chrondrogenic differentiation it is important that the cells do not adhere to the wells. (promocell.com)
During differentiation, the cells form spheroids which float in the medium. (promocell.com)
In the presence of TGF-β, IL-9 may induce differentiation of naive CD4 + T-cells towards Th17 cells, while IL-9 also affects thymus-derived natural Tregs (nTregs) and enhances their suppressive function in vitro [ 26 ]. (biomedcentral.com)

Migration assays showed that MAIT cells and EM T cells migrated toward conditioned medium from placental explants. (frontiersin.org)

Acute graft-versus-host disease (GVHD) occurs in 40% to 60% of recipients of partially matched umbilical cord blood transplantation (UCBT). (umn.edu)
Taken together, these data reveal interesting similarities and differences between tolerant organ and nTreg-treated hematopoietic stem cell transplantation recipients. (umn.edu)
Biology of Blood and Marrow Transplantation , 20 (2), 173-182. (umn.edu)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases (such as Krabbe disease), including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses as well as peroxisome disorders such as X-linked adrenoleukodystrophy. (medscape.com)
In general, transplantation yields the best results when performed early in the course of the disease (ie, in an asymptomatic affected sibling of a child with a lysosomal storage disorder), in centers with experience in performing transplantations to treat inherited metabolic disorders, and in patients healthy enough to tolerate the conditioning and transplantation regimen. (medscape.com)
Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)
1,2,3 Therefore, it is important to understand that switching mobilization reagents during a study may also change the mobilized cell type and their effect on studies relating to transplantation and stem cell research. (cgt.global)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases, including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses. (medscape.com)
A consequence of the latter cell flow was recently demonstrated in a clinical study in cord blood transplantation. (ashpublications.org)
In these studies, however, transplanted cells were followed, if at all, at the site of transplantation, and biodistribution was not an issue. (stemcellslab.net)

Mononuclear cells are blood cells that have a round and single nucleus like lymphocytes and monocytes. (wikipedia.org)
To determine if a specific subpopulation of CB-MNC are responsible for these protective activities, we depleted CB-MNC of various cell types and found that only removal of CB CD14 + monocytes abolished neuroprotection. (biorxiv.org)
These findings suggest that CD14 + monocytes are a critical cell-type when treating HI with CB-MNC. (biorxiv.org)
We identified Lin - HLADR - CD33 + CD16 + cells and CD14 ++ CD16 - monocytes as the main TLR5 producers, especially in samples of conventionally treated patients developing GVHD. (umn.edu)
CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. (thermofisher.com)
Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)

The transit phase is critical as the lymphocytes can undergo programmed cell death (apoptosis) or be activated to start proliferation. (wikipedia.org)
In this study, we evaluated the immunomodulatory cytokine profile and capacity to inhibit T cell proliferation of WJ-MSC progressing to replicative senescence to determine if the expected response is affected. (usp.br)
CONCLUSION: miR-223-3p secreted in serum of KD patients can regulate the expression of FOXP3 and affect the proliferation, apoptosis, and inflammation of cells. (bvsalud.org)
CCK8, scratch test, and migration test were performed to examine the effect of circ7632 on the cell proliferation and migration. (bvsalud.org)
Overexpression of circ7632 significantly increased vimentin and α-SMA expression, decreased ZO-1 expression, and also decreased cell proliferation. (bvsalud.org)
NK cell proliferation, phenotypic expression and cytotoxic activity were evaluated. (springer.com)
For several decades, neovascularization is believed to be accomplished by proliferation of mature endothelial cells residing in the local vessels through the process of angiogenesis [ 6 ]. (hindawi.com)
The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. (nature.com)
However, no inhibitory effect of MSC-Exo on IRBP-specific T cell proliferation was observed. (nature.com)
Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. (cancerindex.org)

the first and currently only approved IEC therapy, autologous CAR-Ts take advantage of the patient's own T cells to fight the tumor. (sai-med.com)
as in autologous CAR-Ts, T cells are collected and engineered ex vivo , in this case, to express engineered TCRs. (sai-med.com)
2 Examples of this approach include blood vessels and cardiovascular substitutes, where autologous vascular cells have been used for this purpose without immune rejection. (ashpublications.org)
We were holding additionally given autologous adult sensory base cells associated with effector T cells. (cyp17-signal.com)

NIHMS1512949-health supplement-4.jpg (110K) GUID:?06D54B37-Compact disc61-49FB-982B-7EFB00148FD4 Abstract Calcitriol, the active type of vitamin D, continues to be well documented to do something in immune system cells and malignant cells straight. (synanet2020.com)

Umbilical cord blood (UCB) specimens were obtained with informed consent from volunteer donors attending the obstetrics department at Ruijin Hospital. (biomedcentral.com)
To compare the heterogeneities of human umbilical cord mesenchymal stem cells (HUCMSCs) derived from different donors and test their therapeutic variations in mouse liver fibrosis model. (researchsquare.com)
Human Mobilized Peripheral Blood Primary Cells are collected from healthy IRB consented donors that are injected with Granulocyte-Colony Stimulating Factor (G-CSF aka Neupogen ® ), Plerixafor (aka Mozobil ® ), or a combination of Plerixafor and G-CSF. (cgt.global)
The presence of immune cells in UCB that are not directed against maternal foreign Ags is remarkable and may explain the reported clinical observation of improved HSCT outcome with younger sibling donors. (ashpublications.org)
3 In this study, we hypothesize that younger siblings are better donors as they might have come into contact with nonshared Ags of their older siblings through transmaternal cell flow. (ashpublications.org)
Examples were acquired from sufferers or healthy donors not on immunosuppressant treatment currently. (synanet2020.com)
25 sera from healthy donors were titrated as controls. (liverpool.ac.uk)

Dendritic cells-based therapeutic approach may be a potential strategy for SARS-CoV-2 infection. (medsci.org)
Basis of treatment based dendritic cells to combat coronavirus infections is summarized. (medsci.org)
Among the first responders to viral infections, tissue-resident macrophages and dendritic cells (DCs) (55) recognize evolutionarily conserved microbial structures termed pathogen-associated molecular patterns (PAMPs) via germline-encoded pattern recognition receptors (PRRs) (56). (debuglies.com)
CD86, also known as B7-2, is a transmembrane glycoprotein that is typically found on APCs such as macrophages, dendritic cells, and B cells. (cusabio.com)

The levels of several chemokines were significantly higher in intervillous compared to peripheral blood, including macrophage migration inhibitory factor (MIF), CXCL10, and CCL25, whereas CCL21, CCL27 and CXCL12 were lower. (frontiersin.org)

The intervillous space of the placenta is a part of the fetal-maternal interface, where maternal blood enters to provide nutrients and gas exchange. (frontiersin.org)
The pregnant woman's arterial blood fills the intervillous space of the placenta, where it comes in direct contact with the fetal villi protruding from the fetal part of the placenta. (frontiersin.org)
Fetal blood vessels run inside the villi, and gas and nutrients are exchanged over a thin membrane of fetal cytotrophoblasts and syncytiotrophoblasts ( 1 ). (frontiersin.org)
The use of fetal bovine serum (FBS) as a cell culture supplement is discouraged by regulatory authorities to limit the risk of zoonoses and xenogeneic immune reactions in the transplanted host. (biomedcentral.com)

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (rndsystems.com)
Activated γδ T cells exhibit strong cytotoxic activity and cytokine secretion functions and are effective antitumor lymphocytes with simple and direct recognition modes and rapid responses. (explorationpub.com)
First, γδ T cells exposed to complicated cytokine networks are potentially affected by multiple inhibitory mechanisms. (explorationpub.com)
The importance of proinflammatory T-cells and their cytokine production in patients with autoimmune arthritis has been widely described. (biomedcentral.com)
Activated T cells are one of the better characterized goals of calcitriol, with results including lowering inflammatory cytokine result and marketing anti-inflammatory cytokine creation. (synanet2020.com)
Reports claim that just immature NK cell populations are influenced C188-9 by calcitriol treatment leading to impaired cytotoxic function and cytokine creation, while mature NK cells may have little if any response. (synanet2020.com)
We previously confirmed the power of calcitriol to diminish STAT1 tyrosine 701 (p-STAT1) and STAT3 tyrosine 705 (p-STAT3) phosphorylation aswell as inflammatory cytokine result of T cell huge granular lymphocyte leukemia cells, but didn't determine the consequences of calcitriol on NK-LGLL. (synanet2020.com)

In a phase I study, adoptive transfer of expanded CD4 + CD25 + Foxp3 + natural regulatory T cells (nTregs) resulted in a reduced incidence of grade II-IV acute GVHD. (umn.edu)
To investigate potential mechanisms responsible for the reduced GVHD risk, we analyzed peripheral blood mononuclear cell mRNA expression of a tolerance gene set previously identified in operation- tolerant kidney transplant recipients, comparing healthy controls and patients who received nTregs and those who did not receive nTregs with and without experiencing GVHD. (umn.edu)
Samples from patients receiving nTregs regardless of GVHD status showed increased expression of Foxp3 expression, as well as B cell-related tolerance marker. (umn.edu)
CD8 + T cells showed reduced signs of activation (HLA-DR + expression) compared with conventionally treated patients developing GVHD. (umn.edu)

During pregnancy, one of the main functions of the placenta is to provide the growing fetus with oxygen and nutrients from the maternal blood circulation. (frontiersin.org)
The circulation of maternal blood in the intervillous space from a physiological perspective has been described ( 3 ). (frontiersin.org)
The potential factors involved in the migration of maternal immune cells to the placenta is still unexplored. (frontiersin.org)

As a result, in today's study, we looked into whether NKL C188-9 cells, a style of NK-LGLL, and NK-LGLL individual peripheral bloodstream mononuclear cells (PBMCs) are vunerable to treatment with calcitriol or seocalcitol (EB1089), a powerful analog of calcitriol. (synanet2020.com)

Many preclinical studies suggest that CB-MNC protect the brain after HI by releasing neurotrophic and anti-inflammatory factors that stimulate repair by host cells [ 8 , 9 ]. (biorxiv.org)
INTRODUCTION: Wharton's jelly mesenchymal stem cells (WJ-MSC) exhibit the ability to modulate T cell responses and these immunomodulatory effects are usually anti-inflammatory. (usp.br)

CGT Global offers PBMCs and CD34+ stem cells mobilized with G-CSF, Plerixafor, and G-CSF plus plerixafor. (cgt.global)

The result of this process is the loss of stem cell functionality, which limits its use for therapeutic purposes. (usp.br)
The translation of stem cells is showing their therapeutic strength for a wide range of intractable and chronic diseases in both clinical and academic studies [1]. (heraldopenaccess.us)

Primary human umbilical vein endothelial cells (HUVECs) were obtained and incubated with 15% HC and KD serum for 48 h. (bvsalud.org)
Quantitative analyses showed the microvessel density was significantly superior to that generated by human dermal microvascular endothelial cells (HDMECs) but similar to that generated by human umbilical vein endothelial cells (HUVECs). (ashpublications.org)

Mucosal-associated invariant T (MAIT) cells and effector memory (EM) T cells were enriched in the intervillous blood compared to peripheral blood, suggesting that MAIT cells and other EM T cells home to the placenta during pregnancy. (frontiersin.org)
However, our recent findings showed that mucosal-associated invariant T (MAIT) cells and effector memory (EM) T cells are enriched in the IVB of term placentas ( 4 ), indicating that certain immune cell subsets are recruited to or retained in the intervillous space. (frontiersin.org)
Immune effector cell (IEC) therapies, commonly referred as cell therapies, utilize human cells to cause or enhance an immune response against tumor cells. (sai-med.com)
γδ T cells recruited into the tumor microenvironment can act as effector cells to mediate cancer immune surveillance. (explorationpub.com)
After an incubation period of 6 days, the proportions of the T-cell subpopulations TEMRA (CD45RA + CD27 − ), memory (CD45RA − CD27 + ), effector (CD45RA − CD27 − ) and naive cells (CD45RA + CD27 + ) were determined. (biomedcentral.com)

When using a cell line made by cloning an original white blood cell (monoclonal antibodies), there is evidence for recirculation of lymphocytes, particularly in null-cell subsets found from the bronchoalveolar spaces back into regional draining lymph nodes. (wikipedia.org)
We found that MSC-Exo greatly reduced the intensity of ongoing EAU as their parent cells by reducing the infiltration of T cell subsets, and other inflammatory cells, in the eyes. (nature.com)
It is of great significance to gain insight into the classification of γδ T cell subsets. (explorationpub.com)

Although these findings have been confirmed using cell lines, there is a growing evidence to suggest that cell lines do not fully recapitulate the biology of human disease. (biomedcentral.com)
International Journal of Biochemistry & Cell Biology, 40 , 2335-2340. (springer.com)
Natural killer cell biology: An update and future directions. (springer.com)

These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)

The number of null cells has increased over time in subpopulations of mononuclear cells. (wikipedia.org)
We also used positively selected subpopulations of CB-MNC and PB-MNC in this assay and demonstrated that purified CB-CD14 + cells, but not CB-PB CD14 + cells, efficiently protected neuronal cells from death and reduced glial activation following OGD. (biorxiv.org)

However, MSC have a limited lifespan in vitro, with a progressive reduction in their capacity for selfrenewal leading to irreversible arrest of cell division. (usp.br)
Information on the variability of individual cell samples impacting upon in vitro expansion, immunomodulatory potential, and aging processes is still lacking. (usp.br)
Forty-two of 104 tetramer pos T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. (ashpublications.org)
This CD86 antibody was made by transfecting human CD86 monoclonal antibody gene-vector clones into a cell line for in vitro expression, then purifying the antibody using affinity chromatography from the tissue culture supernatant (TCS). (cusabio.com)
The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
Endometrial Cells Acutely Exposed to Phthalates In Vitro Do Not Phenocopy Endometriosis. (ivi-rmainnovation.com)

These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)

Cord blood (CB) mononuclear cells (MNC) are being tested in clinical trials to treat hypoxic-ischemic (HI) brain injuries. (biorxiv.org)
Determining which cell types in CB-MNC enhance brain tissue repair, and the mechanisms by which they do so, will optimize decisions on dosing, route of administration, treatment frequency, and other critical clinical and regulatory parameters. (biorxiv.org)
In addition, potential clinical significance of T cell responses has been overlooked during the first waves of the pandemic, calling for additional in-depth studies. (bvsalud.org)
In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
Human umbilical cord platelet-rich plasma to treat endometrial pathologies: methodology, composition and pre-clinical models. (ivi-rmainnovation.com)
However, the clinical application of tumor-infiltrating γδ T cells has certain limitations. (explorationpub.com)
Finally, it retrospectively analyze the activation strategies and clinical relevance of existing γδ T cell adoptive immunotherapies. (explorationpub.com)
Together with our clinical teams, we believe that using your own stem cells are the safest, most effective health care plan for your body. (kellumstemcellinstitute.com)
positive serum tests for infectious mononucleosis (im) unaccompanied by the clinical syndrome or blood changes characteristic of the disease were detected in 39/177 (22%) mentally subnormal patients investigated with three different commercially available im slide tests. (liverpool.ac.uk)
clinical applications of cell surface markers. (liverpool.ac.uk)

In conclusion, female UCB comprises HY-specific cytotoxic T cells. (ashpublications.org)
Targeting METTL3 by single-guide RNA, nanoparticle small interfering RNA (siRNA), or pharmacological inhibitor (STM2457) in combination with anti-programmed cell death protein 1 (PD-1) synergized to reinvigorate cytotoxic CD8+ T cells and mediate tumor regression. (bvsalud.org)

After induction with lipopolysaccharide, the expression of tsGILT mRNA was upregulated in spleen and kidney and recombinant tsGILT protein transferred to late endosomes and lysosomes in HeLa cells. (bvsalud.org)
Infected mice treated with Pam3CSK4 possessed more hepatic inflammation focus, lower IgG and IgG2a antibody titers, and a lower proportion of spleen CD3+CD4+ T cells. (bvsalud.org)

RECENT FINDINGS: The use of umbilical cord-derived mesenchymal stem cells as HDT for COVID-19 has been shown to be safe in phase 1 and 2 trials. (bvsalud.org)

γδ T cells express unique T cell receptor (TCR) γ and TCR δ chains, with structural and functional heterogeneity. (explorationpub.com)
At present, the phenotypic heterogeneity and functional variation of human γδ T cells are increasing. (explorationpub.com)

This enhanced secretion of cholesterol and cholesteryl esters that impair CD8+ T cell function in the tumor microenvironment. (bvsalud.org)
Therefore, an in-depth understanding of γδ T cells that play conflicting roles in the tumor microenvironment is necessary. (explorationpub.com)

At the same time, sRAGE is known to compete for RAGE, the main receptor for AGEs, and it has been shown that their interaction contributes to cell apoptosis, as discussed in this review. (heraldopenaccess.us)

METHODS: Blood samples were collected in acute febrile phase of KD, after IVIG treatment, and from healthy controls. (bvsalud.org)
Twenty-three female UCB samples were collected from healthy mothers and analyzed for minor histocompatibility Ag HY-specific responses. (ashpublications.org)

Functions of natural killer cells. (springer.com)
Natural killer cells: Detectors of stress. (springer.com)
Isolation, expansion, and characterization of natural killer cells and their precursors as a tool to study cancer immunosurveillance. (springer.com)
Understanding of molecular mechanisms in natural killer cell therapy. (springer.com)
Shaping of natural killer cell antitumor activity by ex vivo cultivation. (springer.com)
Natural killer cell adoptive immunotherapy: Coming of age. (springer.com)
Here, we briefly define the four main groups of IECs currently under research: chimeric antigen receptor T cells (CAR-T), engineered T cell receptor (TCR) T cells, tumor-infiltrating lymphocytes (TILs), and natural killer (NK) cells, with a view towards advantages and disadvantages of each approach. (sai-med.com)
In a study published in Science Immunology, researchers at Karolinska Institutet in Sweden show that so-called natural killer (NK) cells were strongly activated early afte r SARS-CoV-2 infection but that the type of activation differed in patients with moderate and severe COVID-19. (debuglies.com)

However, compared to non-COVID-19 pneumonia, COVID-19 pneumonia has been more likely to have a peripheral distribution (80% vs. 57%), ground-glass opacity (91% vs. 68%), fine reticular opacity (56% vs. 22%), and vascular thickening (59% vs. 22%), but COVID-19 pneumonia has been less likely to have a central+peripheral distribution (14. (medscape.com)

Our top-tier doctors stay ahead in regenerative medicine, employing the latest in stem cell therapy to ensure safe, effective care. (kellumstemcellinstitute.com)
Embark on your health journey with Kellum Stem Cell Institute and unlock the transformative potential of stem cell therapy. (kellumstemcellinstitute.com)

With null cells increasing during an immune response, the changes are believed to be due to defects involved with an aging immune system and can be used as a representation of a healthy immune system in the healthy aged group, which is linked to survival. (wikipedia.org)

Peripheral blood specimens were obtained from healthy volunteers with informed consent. (biomedcentral.com)
NK cells were obtained from peripheral blood (PB) of healthy volunteers. (springer.com)

Circulating T cells are collected and engineered ex vivo to express a customized receptor, the CAR, which recognizes and binds to a surface protein (antigen) in the tumor cell, inducing cell death. (sai-med.com)
TILs are collected from a solid tumor sample, separated from the tumor cells, expanded ex vivo , and tested for tumor recognition. (sai-med.com)
their combination with stem cells is among the best alternatives for their continuous generation in vivo . (heraldopenaccess.us)

One example of a gene-edited cell line isthe soluble receptor for advanced glycation end product (sRAGE) secreting stem cells, and much attention is paid to the remedial role of sRAGE molecules in advanced glycation end product (AGE)-associated disorders [3-6]. (heraldopenaccess.us)
CD86 expression is modest in resting B cells, but it rises in response to BCR, CD40, IL-4R, LPS receptor, or 2-adrenergic receptor stimulation. (cusabio.com)
Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)

Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. (thermofisher.com)

Namely, recipients sharing one or more HLA Ags with their cord blood donor inherited paternal Ags had reduced relapse rates compared with those who did not. (ashpublications.org)
The individual or healthy donor age may be the age at the proper time the sample was collected. (synanet2020.com)

Gene expression data from 24 experiments for 8 different cell types of the human myelopoietic lineage were used to generate an integrated myelopoiesis dataset of 9,425 genes, each reliably associated to a unique genomic position and chromosomal coordinate. (biomedcentral.com)
For instance, the existence of tissue-specific gene clusters may be related to the efficient activation of gene expression in a particular cell lineage, by genetic and epigenetic mechanisms, or related to the repression of entire chromosomal regions containing genes expressed in a specific cell type, e.g. during the developmental switches leading to different cell lineages [ 25 ]. (biomedcentral.com)

The type of NK cell activation detected differed considerably in patients with moderate compared to severe disease," says Niklas Björkström, physician and immunology researcher at the Center for Infectious Medicine, Department of Medicine Huddinge, at Karolinska Institutet, who led the study. (debuglies.com)

Umbilical cord blood (UCB) is used for HSCT. (ashpublications.org)

Due to their immunosuppressive potential, MSC have recently emerged as a promising tool for cell therapy. (usp.br)
2014). Human NK cells: From surface receptors to the therapy of leukemias and solid tumors. (springer.com)
Enzyme replacement therapy (ERT) appears safe and effective for peripheral manifestations in patients with Gaucher disease types I and III, Fabry disease, mucopolysaccharidosis I (Hurler, Hurler-Scheie, and Scheie syndromes), mucopolysaccharidosis II (Hunter syndrome), mucopolysaccharidosis VI (Maroteaux-Lamy syndrome), Pompe disease, and recently Batten disease (neuronal ceroid lipofuscinoses, CLN2). (medscape.com)
This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
Enzyme replacement therapy (ERT) appears safe and effective for peripheral manifestations in patients with Gaucher disease types I and III, Fabry disease, mucopolysaccharidosis I (Hurler, Hurler-Scheie, and Scheie syndromes), mucopolysaccharidosis II (Hunter syndrome), mucopolysaccharidosis VI (Maroteaux-Lamy syndrome), and Pompe disease. (medscape.com)
These results suggest that MSC-Exo effectively ameliorate EAU by inhibiting the migration of inflammatory cells, indicating a potential novel therapy of MSC-Exo for uveitis. (nature.com)
Mesenchymal stem cell (MSC)-based therapy for the treatment of autoimmune diseases has demonstrated a particular promise after its successful applications in both animal models and patients. (nature.com)
Functionally improved cell therapy has brought enormous benefits for patients suffering from serious illnesses. (heraldopenaccess.us)

a rapidly emerging field in IEC, NK cells play a role in immunosurveillance and are guided to tumor sites by chemokines and their associated receptors. (sai-med.com)

The study is part of the larger Karolinska COVID-19 Immune Atlas project, which aims to increase knowledge about the characteristics of immune cells in patients with COVID-19. (debuglies.com)
This review further emphasizes the diversified cross-talk between γδ T cells and other immune cells. (explorationpub.com)

However, what factors mediate brain repair, the CB-MNC cell types that contribute, and the host cells with which they interact are unclear. (biorxiv.org)
We are still far from a common definition of PL and standardized PL manufacture due to our limited knowledge of the mechanisms that mediate PL-promoting cell growth. (biomedcentral.com)

Anatomy56

Organisms11

Diseases32

Chemicals and Drugs58

Analytical, Diagnostic and Therapeutic Techniques and Equipment57

Phenomena and Processes44

Disciplines and Occupations1

Information Science3

Named Groups3

Health Care7

Prepared from human umbilical cord1

Progenitor cells7

Bone20

MSCs32

Adipose1

Adult peripheral blood3

Tissues7

Differentiation9

Placental1

Transplantation11

Monocytes6

Proliferation10

Autologous4

Abstract1

Donors7

Dendritic cells4

Macrophage1

Fetal4

Cytokine7

GVHD4

Maternal3

Bloodstream1

Anti-inflammatory2

PBMCs1

Therapeutic2

HUVECs2

Effector5

Subsets3

Biology3

Mature endothelial cells1

Subpopulations2

Vitro8

Endogenous1

Clinical14

Cytotoxic2

Spleen2

Recent findings1

Heterogeneity2

Tumor microenvironment2

Apoptosis1

Samples were collected2

Natural Killer8

Vascular1

Stem cell th2

Survival1

Volunteers2

Vivo3

Receptor3

Processes1

Donor2

Gene2

Immunology1

HSCT1

Therapy8

Chemokines1

Immune cells2

Mediate2