Mononuclear cells cell biology clone cells. Medical search. Frequent questions

Therefore, we developed a novel formula to improve the expansion of peripheral γδ T cells from healthy donors. (nature.com)
γδ T cells, specifically the Vγ9Vδ2 subset, which is the dominate subset among γδ T cells in human peripheral blood, recognize target cells in a human leukocyte antigen (HLA)-independent manner. (nature.com)
Here, we report that plasma exosomes of COVID-19 patients contain SARS-CoV-2 double stranded RNA (dsRNA) and stimulate robust production of interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α), and other inflammatory cytokines and chemokines by human peripheral mononuclear cells. (wphobby.com)
These exosomes significantly increase expression of endosomal toll-like receptor 3 (TLR3), TLR7, TLR8, and TLR9 in peripheral T cells and monocytes. (wphobby.com)
We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
In the current study, phage-exposed mimotopes as targets against tegumentary leishmaniasis (TL) were selected by means of bio-panning cycles employing sera of TL patients and healthy subjects, besides the immune stimulation of peripheral blood mononuclear cells (PBMCs) collected from untreated and treated TL patients and healthy subjects. (cambridge.org)
When evaluating the immunogenicity of LiHyS, which is possibly located in the parasite's flagellar pocket, proliferative assays using peripheral blood mononuclear cells from healthy subjects or VL patients showed a high proliferative index in both individuals, when compared to the results obtained using rA2 or unstimulated cultures. (cambridge.org)
All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock ( BUF070A ). (bio-rad-antibodies.com)
lated from peripheral blood mononuclear cells of a patient a high level of cell death was also observed in C6/36 cells. (cdc.gov)
We have characterized the T cell composition and chemokine profile in paired intervillous and peripheral blood samples from healthy mothers giving birth following term pregnancies. (frontiersin.org)
Mucosal-associated invariant T (MAIT) cells and effector memory (EM) T cells were enriched in the intervillous blood compared to peripheral blood, suggesting that MAIT cells and other EM T cells home to the placenta during pregnancy. (frontiersin.org)
Furthermore, pregnant women had lower proportions of peripheral blood MAIT cells compared to non-pregnant women. (frontiersin.org)
The intervillous blood (IVB) is exchanged 2-3 times per minute ( 1 ), suggesting that the cell composition in IVB may reflect that of peripheral blood (PB). (frontiersin.org)
Staining of porcine peripheral blood lymphocytes with FITC cojugated Mouse anti Porcine CD5 antibody, clone 1H6/8 ( MCA2307F ). (bio-rad-antibodies.com)
Methods We first detected plasma catecholamine concentrations in patients with SLE and healthy controls by mass spectrometry, followed by reverse transcription-quantitative PCR (RT-qPCR) to detect DRD messenger RNA (mRNA) expression in peripheral blood mononuclear cells (PBMCs) and CD4 + T cells, and flow cytometry to detect DRD expression in Tfh cells. (bmj.com)
Purified, unmanipulated NK cells were infused on Day 0, and NK cell chimerism and phenotyping from peripheral blood were performed on Days 7, 14, 21, and 28. (biomedcentral.com)
We present single cell RNA sequence data from 1.3m peripheral blood mononuclear cells from 982 healthy human subjects. (iscb.org)
Lymphoid lineage DCs primarily differentiate into plasmacytoid DCs and occupy approximately 0.5% of peripheral blood mononuclear cells (PBMCs) in humans [ 2 ], but the cell population percentage is unclear in horses. (biomedcentral.com)
Baseline expression of ORMDL1 and ORMDL2 was higher in peripheral blood mononuclear cells from patients with asthma. (medscape.com)
Our results show that the murine thymoma cell line EL-4 is superior to peripheral blood mononuclear cells in both cellular outgrowth and cloning experiments, whereas monocyte-derived factors and monocyte cell lines were less capable than peripheral blood mononuclear cells in enhancing cellular outgrowth and cloning. (lu.se)

Results The plasma dopamine concentration in patients with SLE was significantly increased, and abnormal mRNA expression of DRDs was observed in both PBMCs and CD4 + T cells. (bmj.com)
Used cell isolation techniques to purify PBMCs from blood (Ficoll), select monocytes and CD4 T cells, differentiate and harvest dendritic cells and macrophages. (livecareer.com)
Our frozen PBMCs are isolated within 5 hours of collection and frozen at -80 o C in CryoStor® cell cryopreservation media to ensure minimal degradation and maintain high post-thaw viability. (bioscience.co.uk)

The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
To apply allogeneic Vγ9Vδ2 T cells in adoptive immunotherapy, the methodology used to obtain adequate cell numbers with optimal effector function in vitro needs to be optimized, and clinical safety and efficacy also need to be proven. (nature.com)
We found that the expanded cells possessed significantly improved immune effector functions, including proliferation, differentiation, and cancer cell killing, both in vitro and in the humanized mouse model. (nature.com)
Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
Assessment of stem cell function in vitro as well as in vivo established that only HSCs and not investigated progenitor populations could propagate the SF3B1 mutated clone. (nih.gov)
In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
The clones were evaluated regarding their specific interferon- γ (IFN- γ ) and interleukin-4 (IL-4) production in the in vitro cultures, and selectivity and specificity values were calculated, and those presenting the best results were selected for the in vivo experiments. (cambridge.org)
Furthermore, we conduct a cross-species prediction of cardiomyocyte subtypes from in vitro-derived human induced pluripotent stem cells and unexpectedly uncover a predominance of left ventricular (LV) identity that we confirmed by an LV-specific TBX5 lineage tracing system. (stanford.edu)
The effect of IL-15 stimulation on IL-17 production by BM mononuclear cells was examined in vitro. (biomedcentral.com)
Finally, in vitro experiments and RNA sequencing (RNA-seq) were used to explore the possible pathway by which DRDs regulate Tfh cell differentiation. (bmj.com)
In vitro induction experiments showed that differentiation of naïve T cells into Tfh cells was accompanied by an increase in D1-like receptor expression. (bmj.com)
In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. (biomedcentral.com)
Forty-two of 104 tetramer pos T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. (ashpublications.org)

These data demonstrate that African green monkey-like natural killer cell differentiation profiles can be rescued in rhesus macaques to promote viral clearance in tissues. (nature.com)
define NK cell differentiation states based on their education via NKG2a and expression of CD16 (FcγRIII), an activating Fc receptor that mediates antibody-dependent cell-mediated cytotoxicity (ADCC) 8 , and demonstrate that nonpathogenic SIVagm infection in African green monkeys (AGMs) imprints the maturation of NK cells inducing terminally differentiated NKG2a low CD16 + NK cells, which express high levels of interleukin (IL)−21R. (nature.com)
Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
The power of the thiol proteinases to cleave a multitude of substrates in response to calcium mineral activation allows their involvement in a variety of cell processes offering cell motility, sign transduction, apoptosis, cell differentiation and legislation of the cytoskeleton (3). (exposed-skin-care.net)
Human cytomegalovirus (CMV) infection is reported to promote NK cell differentiation and education. (aacrjournals.org)
Together, our work provides the scientific community with a powerful new genetic lineage tracing approach as well as a single cell transcriptomic atlas of hiPSCs undergoing cardiac differentiation. (stanford.edu)
To verify this hypothesis, we examined the frequency of Th1, Th2 and Th17 cell populations as well as the concentrations of cytokines involved in Th17 cell differentiation and migration in BM from RA and OA patients. (biomedcentral.com)
Therefore, elucidating the mechanism of Tfh cell differentiation will contribute to SLE treatment. (bmj.com)
Previous studies have found that DRDs can regulate differentiation of immune cells. (bmj.com)
RNA-seq and RT-qPCR results indicate that D1-like receptors might promote Tfh cell differentiation through the Phosphatidylinositol3-kinase (PI3K)/protein kinase B (AKT)/Forkhead box protein O1 (FOXO1)/Kruppel-like factor 2 (Klf2) pathway. (bmj.com)
D1-like receptors may promote Tfh cell differentiation through the PI3K/AKT/FOXO1/Klf2 pathway. (bmj.com)
Furthermore, the human T cell hybridoma cell line MP6 that secretes a B cell growth and differentiation factor, recently identified as an isoform of thioredoxin, is also capable of stimulating EBV-infected B cells and lymphoblastoid cell lines. (lu.se)

Following verification of a stem-like signature (upregulated expression of stemness markers, resistance to chemotherapy, increased spheroid formation, and tumorigenesis capabilities despite inoculation of a small number of cells), we analyzed the interaction of these cells with macrophages via direct and indirect coculture assays. (springer.com)
Migration assays showed that MAIT cells and EM T cells migrated toward conditioned medium from placental explants. (frontiersin.org)
Blocking of MIF or a combination of MIF, CCL25, and CCL20 in migration assays inhibited MAIT cell migration toward placenta conditioned medium. (frontiersin.org)
The antiviral activity of Bi121 was also assessed against SARS-CoV-2 variants (USA WA1/2020, Hongkong/VM20001061/2020, B.1.167.2 (Delta), and Omicron) in Vero cells and HEK-ACE2 cell lines using RT-qPCR and plaque assays. (bvsalud.org)

furthermore, in vivo IFNα-induced NK cell cytotoxicity correlates with reductions in HIV-DNA during antiretroviral therapy (ART) 16 . (nature.com)
In SIVagm infection, systemic IL-15 was associated with NK cell proliferation in lymph node (LN), while systemic IFNα correlated with NK cell cytotoxicity in LN 17 . (nature.com)
Activating receptors contain cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAM) and activate the FcgR-expressing cell to mediate functions including antibody-dependant cell mediated cytotoxicity (ADCC) and phagocytosis of the antibody-labeled target cell. (sanguinebio.com)
The mechanisms of action by which RTX kills FL cells include complement-dependent cytotoxicity (CDC) ( 3 ), antibody-dependent cellular cytotoxicity (ADCC) ( 4 , 5 )-herein referred to as 'effector function' mechanisms-as well as CD20 signaling effects ( 6 ). (spandidos-publications.com)
In addition, M-CSF enhances monocyte functions such asphagocytic activity, microbial killing, and tumor cell cytotoxicity. (biolegend.com)
Unlike cytotoxic T cells, they do not require prior sensitization to eliminate tumor cells but mediate cytotoxicity through complex interactions between NK cell surface receptors and their cognate ligands expressed on their respective target cells [ 6 ]. (biomedcentral.com)

CCL2 tightly regulates cellular mechanics and thereby recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. (wikipedia.org)
CCL2 is primarily secreted by monocytes, macrophages and dendritic cells. (wikipedia.org)
Moreover, γδ T cells can directly kill target cells without the involvement of dendritic cells (DCs) and perform dual functional roles in antitumor and anti-infective immunity. (nature.com)
These exosomes can interact with immune cells, such as T cells, macrophages, and dendritic cells, to modulate proinflammatory immune responses. (wphobby.com)
Zinc Induces Dendritic Cell Tolerogenic Phenotype and Skews Regulatory T Cell-Th17 Balance. (uc.edu)
Dendritic cells (DCs) are professional antigen-presenting cells that have multiple subpopulations with different phenotypes and immune functions. (biomedcentral.com)
Dendritic cells (DCs) are the most important antigen-presenting cells (APCs) in the body. (biomedcentral.com)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

These changes are thought to lead to tumor-microenvironment interactions via factors secreted by stromal or epithelial cells in response to lymphoma cell-produced cytokines ( 7 ). (spandidos-publications.com)
We have here examined various cell-derived cytokines for their ability to enhance both the cellular outgrowth of newly infected B cells and the clonability of infected B cells and lymphoblastoid cell lines. (lu.se)

Administration of anti-CCL2 antibodies in a model of glomerulonephritis reduces infiltration of macrophages and T cells, reduces crescent formation, as well as scarring and renal impairment. (wikipedia.org)
NOS2 is expressed by myeloid-derived suppressor cells and M1 macrophages but not alternatively activated M2 macrophages. (thermofisher.com)
In tumors which had high levels of infiltrating CD11b + macrophages expressing the ADCC-activating FcgRIV, T REGS were selectively depleted in an FcgR-dependant manner, while effector T cells were instead expanded. (sanguinebio.com)
In lymph nodes lacking significant levels of these macrophages, frequencies of both effector T cells and T REGS were increased. (sanguinebio.com)
Tumor-associated T REGS expressedhigher levels of CTLA-4 than their effector T cell counterparts, or than T REGS present in the lymph node, indicating that higher CTLA-4 expression levels mediate ADCC via macrophages in the tumor. (sanguinebio.com)

Both MCP-1 and RANTES induce formation of TRAP-positive, multinuclear cells from M-CSF-treated monocytes in the absence of RANKL, but produced osteoclasts that lacked cathepsin K expression and resorptive capacity. (wikipedia.org)
COVID-19 plasma exosomes induced proinflammatory responses in CD4 + T cells, CD8 + T cells, and CD14 + monocytes but not significantly in regulatory T cells, Th17 T cells, or central memory T cells. (wphobby.com)
A pharmacological inhibitor of TLR3 considerably reduced cytokine and chemokine production by CD4 + and CD8 + T cells but not by CD14 + monocytes, highlighting divergent signaling pathways of immune cells in response to COVID-19 plasma exosomes. (wphobby.com)
However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
Particularly, NME is an idiopathic inflammatory disease of the CNS that is characterized by prominent necrosis and infiltration of inflammatory cells, including lymphocytes, plasma cells, and monocytes or histiocytes into the cerebral cortex and/or white matter, hippocampus, thalamus, and leptomeninges. (biomedcentral.com)

Even though GITR-activation in effector T cells promotes activities including cytokine production and proliferation, the agonistic properties of this antibody alone were not effective in the absence of activating FcgR engagement. (sanguinebio.com)
Recombinant rat M-CSF induces the proliferation of M-NFS-60 cells in a dose dependent manner. (biolegend.com)
ED 50 = 2.5 - 12.5 ng/mL, corresponding to a specific activity of 0.8 - 4.0 x 10 5 units/mg, as determined by induction of N-MSF-60 mouse myelogenous leukemia lymphoblast cell proliferation. (biolegend.com)
Patients may alternatively receive lympho-depleting chemotherapy followed by adoptive transfer of purified NK cells from an alloreactive donor, resulting in homeostatic lymphocyte proliferation with transient donor NK cell expansion in recipients [ 7 ]. (biomedcentral.com)
Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and is widely used as a tool for the establishment of B cell lines producing human monoclonal antibodies. (lu.se)

Percentage of IL-17-producing cells in BM was evaluated by flow cytometry. (biomedcentral.com)
The results of flow cytometry showed that D1-like receptors were highly expressed in Tfh cells of patients with SLE and associated with disease activity. (bmj.com)

Vγ9Vδ2 T cells are promising candidates for cellular tumor immunotherapy. (nature.com)
Together, our results show devCellPy to be a useful tool for automated cell prediction across complex cellular hierarchies, species, and experimental systems. (stanford.edu)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
As described below, these diverse situations range from the complications of diabetes and cellular perturbation in amyloidoses to immune and inflammatory responses and tumor cell behavior. (jci.org)
The cellular FLICE-inhibitory protein (c-FLIP) is a modulator of death receptor-mediated apoptosis and plays a major role in T- and B-cell homeostasis. (ashpublications.org)

For example, ex vivo IL-21 treatment expanded CD16 + NK cells 9 , antagonized the IL-15-dependent expansion of resting NK cells 10 , and reverses hypo-responsiveness via the STAT1 and PI3K-AKT-FOXO1 pathways 11 . (nature.com)
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)

however, despite its prevalence in FL and other cancers, the role of CA125 in tumor biology and immunity has been only recently elucidated. (spandidos-publications.com)
Th1 cells that produce IL-2, IFN- γ , TNF- α, and TNF- β evoke cell-mediated immunity and phagocyte-dependent inflammation while Th2 cells that produce IL-5, IL-4, IL-6, IL-10, and IL-13 induce a strong antibody response that comprises the IgE class and eosinophil accumulation, but it inhibits several functions of phagocytic cells [ 7 ]. (hindawi.com)
[ 2 ] These genes are involved in a variety of biologic processes: Th2 inflammation, T-reg cell function, the HLA locus/immunity, and IgE response of B cells. (medscape.com)

In the human osteoclasts, CCL2 and RANTES (regulated on activation normal T cell expressed and secreted). (wikipedia.org)
Here we report, using the pathogenic model of antiretroviral therapy-treated, SIV-infected rhesus macaques that sequential interleukin-21 and interferon alpha therapy generate terminally differentiated blood natural killer cells (NKG2a/c low CD16 + ) with potent human leukocyte antigen-E-restricted activity in response to SIV envelope peptides. (nature.com)
NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
Viral infection primarily reflects binding between its membrane glycoprotein spike and the angiotensin-converting enzyme 2 (ACE2) receptor on select host human cell surfaces 15 . (wphobby.com)
Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. (bio-rad-antibodies.com)
We report the first laboratory-confirmed human infec- lected and used to infect fresh cell cultures. (cdc.gov)
Human induced pluripotent stem cells (hiPSCs) present an exciting alternative for modeling early human embryogenesis due to their well-established ability to differentiate into all embryonic germ layers. (stanford.edu)
Interleukin-17 is predominantly expressed by a specific subset of human T-helper cells-Th17 cells. (biomedcentral.com)
It is believed that the isolation and characterization of such broadly neutralizing antibodies (bnAbs) from different HIV-1-infected donors are critical for understanding human B cell-mediated immune response to HIV-1 infection and for designing immunogens that can elicit bnAbs by vaccination. (oncotarget.com)
We determined if adoptive transfer of natural killer (NK) cells from haploidentical killer immunoglobulin-like receptor (KIR)-human leukocyte antigen (HLA)-mismatched donors may prolong event-free survival in children with intermediate-risk AML who were in first complete remission after chemotherapy. (biomedcentral.com)
The first evidence for the therapeutic effectiveness of natural killer (NK) cells in AML was established in a study of 57 adult patients, in which none of the 20 individuals who received haploidentical cell transplantation from a killer immunoglobulin-like receptor (KIR)-human leukocyte antigen (HLA)-mismatched donor experienced disease relapse [ 2 ]. (biomedcentral.com)
They are not to be used for any form of human treatment, or for any purpose that is prohibited by law, including any use for human reproductive cloning. (bioscience.co.uk)
Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

CCR2 and CCR4 are two cell surface receptors that bind CCL2. (wikipedia.org)
Conclusion Tfh cells in patients with SLE highly express D1-like receptors, which correlate with disease activity. (bmj.com)
The inhibitory KIRs 2DL1 (CD158a), 2DL2/3 (CD158b), and 3DL1 (CD158e1) are such surface receptors and allow adoptively transferred donor NK cells to exert potent antileukemic effects if the cognate HLA class I ligands are absent in the recipients [ 3 ]. (biomedcentral.com)

Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
Donor NK cell kinetics were determined as secondary endpoints. (biomedcentral.com)
The lack of benefit may result from insufficient numbers and limited persistence of alloreactive donor NK cells but does not preclude its potential usefulness during other phases of therapy, or in combination with other immunotherapeutic agents. (biomedcentral.com)

Approximately one-third of children with acute myeloid leukemia (AML) experience disease relapse despite risk-adapted intensive multiagent chemotherapy with or without hematopoietic cell transplantation (HCT) [ 1 ]. (biomedcentral.com)

Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
Thus, even for functionally different (antagonistic versus agonistic) immunotherapeutic antibodies targeting these same T cell populations, FcgR-mediated ADCC of T REG s appears to be a critical mechanism for anti-tumor effects. (sanguinebio.com)

Unlike HIV infection, which progresses to AIDS absent suppressive anti-retroviral therapy, nonpathogenic infections in natural hosts, such African green monkeys, are characterized by a lack of gut microbial translocation and robust secondary lymphoid natural killer cell responses resulting in an absence of chronic inflammation and limited SIV dissemination in lymph node B-cell follicles. (nature.com)
Virus-infected cells release virus-associated exosomes, extracellular vesicles of endocytic origin, into the blood to deliver viral cargoes able to regulate immune responses. (wphobby.com)
In the course of RA, the BM microenvironment can promote the development of Th17 cell responses and overproduction of IL-17AF that may lead to increased inflammation and tissue destruction in RA BM. (biomedcentral.com)
The development of immune checkpoint blockers (ICB) aimed at enhancing antitumor T-cell responses has revolutionized cancer treatment. (aacrjournals.org)
We investigated whether B cell priming from RV144 vaccination impacted functional antibody responses to HIV-1 following infection. (bvsalud.org)
These data reveal that RV144 vaccination primed B cells towards specific binding and functional antibody responses following HIV-1 infection. (bvsalud.org)

Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
We investigated the origin of SF3B1 mutations within the bone marrow hematopoietic stem and progenitor cell compartments in patients with MDS-RS. (nih.gov)

Used Western Blotting to check protein phosphorylation of different cell signaling pathways. (livecareer.com)

CTLA-4 is related to the T cell co-stimulatory receptor CD28, and acts to suppress T cell function by competing with CD28 for binding to CD80 and CD86 on antigen presenting cells and recruiting inhibitory molecules into the TCR signaling synapse. (sanguinebio.com)

In conclusion, female UCB comprises HY-specific cytotoxic T cells. (ashpublications.org)

Given previous findings on a role of IL-21 and IFNα in regulating NK cell function, and that NKG2a low CD16 + NK cells are generated while expressing high levels of IL-21R in nonpathogenic SIV infection, we sought to determine whether immunotherapy with IL-21 and IFNα rescues AGM-like profiles of NK cell maturation and activity in SIV-infected rhesus macaques (RMs). (nature.com)
The impact of CMV infection and adaptive-like NK cells on relapse and survival of patients with AML not receiving allo-SCT remains unknown. (aacrjournals.org)
Tumor Necrosis Factor Alpha Antagonism Reveals a Gut/Lung Axis That Amplifies Regulatory T Cells in a Pulmonary Fungal Infection. (uc.edu)
Two clones, namely A4 and A8, were identified and used in immunization protocols from BALB/c mice to protect against Leishmania amazonensis infection. (cambridge.org)
Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine examines all aspects of M. tuberculosis biology, transmission, and infection, as well as ongoing strategies to treat and control it. (cshlpress.com)
RV144 vaccination limited the development of broadly neutralizing antibodies post-infection, but enhanced Fc-mediated effector functions indicating B cell priming by RV144 vaccination impacted downstream antibody function. (bvsalud.org)
Single-Cell Analysis of RNA Virus Infection Identifies Multiple Genetically Diverse Viral Genomes within Single Infectious Units. (cdc.gov)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
Little is known about the maternal immune cells at this site, which are in direct contact with fetal tissues. (frontiersin.org)
Together, these findings indicate that term placental tissues attract MAIT cells, and that this effect is at least partly mediated by MIF. (frontiersin.org)
Another theory sug- perature, blood pressure, and noting signs gests that circulating immune complexes of liver cell failure such as pallor, jaundice, composed of HCV antigens and antibodies palmar erythema, fetor hepaticus and flap- deposit in tissues and initiate an inflamma- ping tremor. (who.int)

The CMV-induced generation of highly differentiated adaptive-like NK cells has been proposed to affect favorably on the maintenance of remission in patients with acute myeloid leukemia (AML) after allogeneic stem cell transplantation (allo-SCT). (aacrjournals.org)
A consequence of the latter cell flow was recently demonstrated in a clinical study in cord blood transplantation. (ashpublications.org)

However, little is known about the biology of this cytokine in BM. (biomedcentral.com)

HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
Previous studies have juxtaposed and analyzed immune cell compartments between different CCA categorizations such as short or long-term overall survival (OS) [ 16 ]. (springer.com)
However, our recent findings showed that mucosal-associated invariant T (MAIT) cells and effector memory (EM) T cells are enriched in the IVB of term placentas ( 4 ), indicating that certain immune cell subsets are recruited to or retained in the intervillous space. (frontiersin.org)

CCL2 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. (wikipedia.org)
Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
The process of neovascularization can be influenced not only by the residual endothelial cells but also by the circulating cells. (hindawi.com)
Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. (thermofisher.com)

CTLA-4 is a negative regulatory molecule expressed by activated T cells as well as by negative regulatory T cells (T REG s). (sanguinebio.com)

While in agreement with previous studies, little or no evidence for clonal ( SF3B1 mutation) involvement could be found in mature B cells, consistent involvement at the pro-B-cell progenitor stage was established, providing definitive evidence for SF3B1 mutations targeting lymphomyeloid HSCs and compatible with mutated SF3B1 negatively affecting lymphoid development. (nih.gov)
Replication of examinations such as patient's mental state, the virus in lymphoid cells may cause extra- build, pulse rate, respiratory rate, body tem- hepatic manifestations. (who.int)

Among these 132 patients, 8 liver cancer patients and 10 lung cancer patients who received ≥5 cell infusions showed greatly prolonged survival, which preliminarily verified the efficacy of allogeneic Vγ9Vδ2 T-cell therapy. (nature.com)
Our clinical studies underscore the safety and efficacy of allogeneic Vγ9Vδ2 T-cell immunotherapy, which will inspire further clinical investigations and eventually benefit cancer patients. (nature.com)
In this respect, the introduction of checkpoint inhibitors to unleash the activity of tumor-reactive T cells has been a milestone in cancer immunotherapy. (nature.com)
Cancer stem cells (CSCs) are thought to the underlying reason for cancer initiation, metastasis, and relapse. (springer.com)
However, due to their elusive character and differences in identification among different types of cancer, it remains a challenge to study such cells. (springer.com)
Here, we employ a fluorescent reporter system to track and isolate stem-like cancer cells of cholangiocarcinoma cell lines. (springer.com)
Cancer stem cells (CSCs) represent a small subset of the tumor population and reside at the apex of the hierarchy [ 9 ]. (springer.com)
High levels of M-CSF, mononuclear phagocytes, and VEGF are associated with poor prognosis in patients with cancer. (biolegend.com)
T cells play a critical role in the control of cancer. (aacrjournals.org)
Cultured and maintained B cells and cancer cells. (livecareer.com)
Utilized MTT assay on different cancer cells to determine the synergistic effect between different compounds synthesized in the lab. (livecareer.com)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

Concordant with these findings, significantly increased percentages of CD3 + CD4 + IL-17 + and CD3 + CD4 + IL-17 + IFN-γ + cells were present in RA BM in comparison to OA BM samples. (biomedcentral.com)
Co-cultivation of EBV-infected B cells with MP6 cells significantly enhanced the cloning efficiency at the 1 cell/well level. (lu.se)

Additionally, characterization of the tumor microenvironment such as interactions with immune cells remain largely unknown. (springer.com)
Apart from CSCs, there are a myriad of factors and interactions between various cell types and the tumor microenvironment (TME) that ultimately affect CCA progression. (springer.com)
Ipilimumab functions to increase the ratio of effector T cells to T REGS in the tumor microenvironment and has been shown to require binding to both types of T cells for maximal anti-tumor effectiveness. (sanguinebio.com)

In the present study we hypothesized that the RA BM microenvironment supports the development of Th17 cells and Th17 cell response. (biomedcentral.com)

the understanding of MSC biology, their mechanism of action (MoA) in different biological contexts, and their targeted and routine use in the clinics is limited ( Hoogduijn and Lombardo, 2019 ). (frontiersin.org)

Finally, MAIT cells showed migratory capacities toward recombinant MIF. (frontiersin.org)

All experiments performed on red cell lysed porcine blood gated on mononuclear cells. (bio-rad-antibodies.com)

Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)

2 Haematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. (nih.gov)
All recurrent mutations identified in mononuclear cells could be tracked back to the phenotypically defined hematopoietic stem cell (HSC) compartment in all investigated patients and were also present in downstream myeloid and erythroid progenitor cells. (nih.gov)
demonstrate through studies on induced pluripotent stem cell-derived cortical neurons that miR-33a is a potential therapeutic target for the treatment of SPG4-related hereditary spastic paraplegia. (portlandpress.com)

The number and function of proangiogenic cells are impaired in patients with diabetes or cardiovascular diseases. (hindawi.com)
Screening for recurrently mutated genes in the mononuclear cell fraction revealed mutations in SF3B1 in 39 of 40 cases (97.5%), combined with TET2 and DNMT3A in 11 (28%) and 6 (15%) patients, respectively. (nih.gov)
HLA-identical sibling stem cells are used for the treatment of patients suffering from various hematologic malignant and nonmalignant diseases. (ashpublications.org)
As primary endpoint, the event-free survival was compared to a cohort of 55 patients who completed chemotherapy and were in first complete remission but did not receive NK cells. (biomedcentral.com)
Twenty-one patients (median age at diagnosis, 6.0 years [range, 0.1-15.3 years]) received a median of 12.5 × 10 6 NK cells/kg (range, 3.6-62.2 × 10 6 cells/kg) without major side effects. (biomedcentral.com)
This has led to the development of two therapeutic approaches to harness the antileukemic effect of alloreactive NK cells for patients with AML. (biomedcentral.com)

Mechanistic studies of the toxicity of zinc gluconate in the olfactory neuronal cell line Odora. (uc.edu)

Natural killer (NK) cells are "licensed" with functional competence following education with self-major histocompatibility complex (MHC) class I molecules 1 . (nature.com)
Vγ9Vδ2 T cells recognize pyrophosphates secreted by many microbes or overproduced by malignant cells in the context of butyrophilin 3A1 molecules. (nature.com)

Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)

Single-cell multi-omics are powerful tools in understanding cell subsets/phenotypes. (stanford.edu)

CCL2 expression in glial cells is increased in epilepsy, brain ischemia Alzheimer's disease experimental autoimmune encephalomyelitis (EAE), and traumatic brain injury. (wikipedia.org)
Mesenchymal stem cells (MSCs) are under investigation to treat autoimmune and degenerative disorders due to their immunomodulatory and regenerative properties. (biomedcentral.com)

Investigation of an optimal cell lysis method for the study of the zinc metalloproteome of Histoplasma capsulatum. (uc.edu)

Used molecular Biology technique to sub-clone the gene of interest. (livecareer.com)
For 14 cell types, we identified 26,597 independent cis-expression quantitative trait loci (eQTLs), and 62,305 trans-eQTL, with the majority showing cell type specific effects on gene expression. (iscb.org)

Anatomy49

Organisms15

Diseases7

Chemicals and Drugs57

Analytical, Diagnostic and Therapeutic Techniques and Equipment42

Phenomena and Processes60

Disciplines and Occupations20

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture6

Humanities4

Information Science8

Named Groups1

Health Care3

Peripheral20

PBMCs3

Vitro15

Differentiation12

Assays4

Cytotoxicity6

Dendritic8

Cytokines2

Macrophages5

Monocytes5

Proliferation5

Flow Cytometry2

Cellular5

Vivo2

Immunity3

Human17

Receptors3

Donor3

Hematopoietic cell transplantation1

Populations2

Responses6

Bone marrow3

Pathways1

Antigen prese1

Cytotoxic T cel1

Infection8

Tissues4

Transplantation2

Cytokine1

Immune cell3

Endothelial5

Regulatory T cel1

Lymphoid2

Cancer13

Significantly2

Tumor microenvironment3

Microenvironment1

20191

Recombinant1

Experiments performed1

HSPCs3

Stem cell3

Patients6

Neuronal1

Molecules2

Stromal1

Subsets1

Autoimmune2

Lysis1

Gene2