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  • guanine
  • They shuttle between the GDP-bound inactive state and the GTP-bound activated state and their activation is predominantly mediated by a family of guanine nucleotide exchange factors (GEFs) referred to as ROPGEFs. (pnas.org)
  • Nup60p also binds Gsp1p-GTP and its guanine nucleotide exchange factor Prp20p, and functions as a Gsp1p guanine nucleotide dissociation inhibitor by reducing the activity of Prp20p. (nih.gov)
  • Their activity is controlled by GTPase activating proteins, guanine nucleotide exchange factors, and GDP dissociation inhibitors. (nature.com)
  • metabolism
  • In cancer tissues, TTT-RUVBL complex mRNAs were elevated and positively correlated with transcripts encoding proteins of anabolic metabolism and mitochondrial function-all mTORC1-regulated processes. (nih.gov)
  • cell adhesion
  • Batch-transfected cells as well as selected cell clones, expressing different levels of rab31 protein, were analyzed with regard to proliferation, cell adhesion, the invasive capacity of tumor cells, and in vivo in a xenograft tumor model. (springermedizin.de)
  • Golgi
  • Anderson disease or chylomicron retention disorder (CMRD), 1 a disease characterized by malabsorption of lipids from the diet and accumulation of chylomicrons in the enterocytes, results from mutations in SAR1B , a component of coat protein complex II (COPII)-coated vesicles that bud from the surface of the ER and transport cargo proteins to the Golgi apparatus. (bloodjournal.org)
  • 14 The ER and Golgi apparatus are not only the initial stations of the secretory pathway, but also the common organelles through which all secreted proteins navigate while advancing toward their final destinations. (bloodjournal.org)
  • Here, the authors report that amino acids also affect membrane trafficking by stimulating endosome-to-Golgi retrograde trafficking and regulating cell surface localization of certain Golgi proteins through Ragulator and Arl5. (nature.com)
  • eukaryotes
  • The available data concerning the involvement of these mG-proteins in the control of vesicle trafficking agree generally with the paradigms accepted for other eukaryotes. (springer.com)
  • doi:10.1016/j .protis.201 0.05.001 Konstanzer Online-Publikations-System (KOPS) URN: http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-129161 URL: http://kops.ub.uni-konstanz.de/volltexte/2011/ 12916 498 compare with higher eukaryotes, considering the complex diversification vesicles and their proteins have achieved during evolution. (docme.ru)
  • yeast
  • Yeast lacking Nup60p also fail to anchor Nup2p at the NPC, resulting in the mislocalization of Nup2p to the nucleoplasm and cytoplasm.Gsp1p-GTP enhances by 10-fold the affinity between Nup60p and Nup2p, and restores binding of Nup2p-Kap60p complexes to Nup60p.The results suggest a dynamic interaction, controlled by the nucleoplasmic concentration of Gsp1p-GTP, between Nup60p and Nup2p at the NPC. (nih.gov)
  • Yeast lacking Nup60p exhibit minor defects in nuclear export of Kap60p, nuclear import of Kap95p-Kap60p-dependent cargoes, and diffusion of small proteins across the NPC. (nih.gov)
  • C) Gsp1p-GTP enhances binding of Nup2p to Nup60p in yeast extracts. (nih.gov)
  • GST-Nup60p (1 μg) was immobilized on beads and was incubated with yeast extract (∼1 mg) supplemented with 1.25 μM recombinant Gsp1p-GTP (Q71L), 0.5 μM recombinant Kap60p, or no additional protein. (nih.gov)
  • Note that addition of Gsp1p-GTP to yeast extract increases by ∼65% the amount of Nup2p bound to Nup60p-coated beads. (nih.gov)
  • The Rab/Ypt small G proteins are essential for intracellular vesicle trafficking in mammals and yeast. (semanticscholar.org)
  • Gallwitz D, Donath C, Sander C. A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product. (springer.com)
  • homology
  • has shown that, in tomato ( Lycopersicon esculentum ) fruit, the transcription of a gene, which showed high homology to a monomeric G-protein from pea, is rapidly but transiently up-regulated by ethylene. (plantphysiol.org)
  • interaction
  • The results suggest a dynamic interaction, controlled by the nucleoplasmic concentration of Gsp1p-GTP, between Nup60p and Nup2p at the NPC. (nih.gov)
  • Gsp1p-GTP and Kap60p modulate the interaction between Nup60p and Nup2p. (nih.gov)
  • Note that Nup2p binds Nup60p, that Kap60p prevents the interaction, and that the NH2 terminus of Nup2p is not required for binding Nup60p. (nih.gov)
  • B) Effect of Gsp1p-GTP on the interaction between Nup2p and Nup60p. (nih.gov)
  • Note that Kap60p interferes with the interaction of Nup2p with Nup60p, but that the presence of Gsp1p-GTP restores binding and promotes formation of Nup60p-Gsp1p-Nup2p-Kap60p complexes. (nih.gov)
  • There is evidence suggesting that PLD acts downstream from G-proteins, but a direct interaction of specific members has not been shown. (embl-heidelberg.de)
  • At the N-terminal region, it has a coiled-coil motif that confers self-interaction properties to the protein and allows it to appear as an oligomer in the testis. (biomedcentral.com)
  • inhibitors
  • Nup60p-coated beads were incubated with various concentrations of radiolabeled Nup2p for 2 h at 25°C in binding buffer with 10 mg/ml BSA and protease inhibitors. (nih.gov)
  • coiled-coil
  • Several RAC/ROP effectors have been identified (see Fig. S1 A ). These include a family of CRIB-domain proteins (RICs) and a coiled-coil domain protein (ICR1). (pnas.org)
  • Gem binds ROKbeta independently of RhoA in the ROKbeta coiled-coil region adjacent to the Rho binding domain. (nih.gov)
  • Fluorescence
  • More detailed serial and tomographic electron microscopy has shown that a green fluorescence protein-DFCP-1 fusion is localized to the tubular vesicular structure adjacent to the isolation membrane, representing at least part of the omegasome [ 8 ] ( Figure 1 ). (biochemsoctrans.org)
  • complexes
  • Gsp1p-GTP enhances by 10-fold the affinity between Nup60p and Nup2p, and restores binding of Nup2p-Kap60p complexes to Nup60p. (nih.gov)
  • Affinity
  • D) Gsp1p-GTP increases the affinity between Nup60p and Nup2p. (nih.gov)
  • In the present paper, a PLD cDNA clone was isolated from tobacco, expressed as a GST fusion in bacteria, and the recombinant protein was purified by glutathione affinity. (embl-heidelberg.de)
  • membrane-bound
  • Here the authors present the 4.9 Å resolution cryo-tomography and subtomogram averaging structure of the membrane bound COPII inner coat that was obtained by in vitro reconstitution and discuss mechanistic implications. (nature.com)
  • antibodies
  • Polyclonal antibodies directed to recombinantly expressed rab31 were generated and protein expression analyzed by immunohistochemistry, Western blot analysis, and a newly developed sensitive ELISA. (springermedizin.de)
  • molecular
  • The enzyme has a monomeric structure with a molecular size of about 37 kDa, being the smallest among the enzymes so far reported. (embl-heidelberg.de)
  • in: Protist 161 (2010), 4, pp. 497-516 doi:10.1016/j.protis.2010.05.001 How to Design a Highly Organized Cell: An Unexpectedly High Number of Widely Diversified SNARE Proteins Positioned at Strategic Sites in the Ciliate, Paramecium tetraurelia Introduction There are only scattered data available on molecular aspects of vesicle trafficking in protozoa, notably in ciliates. (docme.ru)
  • COPII
  • 15 ⇓ - 17 The core components of the COPII coat form 2 layers: an inner layer composed of the monomeric GTP-binding protein Sar1p and the heterodimeric complex Sec23p-Sec24p, and an outer layer formed by the heterotetrameric complex Sec13p-Sec31p. (bloodjournal.org)
  • lysosomal
  • Cells were coimmunostained for mTOR (green) and the lysosomal protein LAMP1 (red), and then processed for images. (nih.gov)
  • nuclear
  • The nucleoporin Nup60p functions as a Gsp1p-GTP-sensitive tether for Nup2p at the nuclear pore complex. (nih.gov)
  • TSG has a bipartite nuclear localization signal that targets the protein to the nucleus. (biomedcentral.com)
  • Finally, export to the cytoplasm requires the addition of the export adapter Nmd3p (11, 20) that provides the leucine-rich nuclear export sequence recognized by Crm1 in a Ran-GTP-dependent fashion. (asm.org)