• Anti-CD44 (SP37) is a recombinant monoclonal antibody produced from supernatant material. (roche.com)
  • That's why we've invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality. (stratech.co.uk)
  • With purified recombinant protein production and peptide synthesis to display limited/selected epitopes, intrinsic factors that can affect the functioning of resulting antibodies can be more easily selected for. (preprints.org)
  • The JUN recombinant monoclonal antibody is prepared using a combination of protein technology and DNA recombinant technology. (cusabio.com)
  • The JUN recombinant monoclonal antibody is then purified from the supernatant of the cell culture through affinity chromatography. (cusabio.com)
  • CD44 antigen isoform 1 precursor. (biossusa.com)
  • The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. (wikipedia.org)
  • In humans, the CD44 antigen is encoded by the CD44 gene on chromosome 11. (wikipedia.org)
  • CD44 has been referred to as HCAM (homing cell adhesion molecule), Pgp-1 (phagocytic glycoprotein-1), Hermes antigen, lymphocyte homing receptor, ECM-III, and HUTCH-1. (wikipedia.org)
  • This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • 1983) used a murine monoclonal antibody (A3D8) to identify an erythrocyte antigen inhibited by the In(Lu) gene. (wdv.com)
  • Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human CD44 antibody ( MCA89 ) followed by HRP-polymer detection and DAB substrate development (low power) following antigen retrieval using citrate buffer at pH6.2. (bio-rad-antibodies.com)
  • Many of these factors stem from the location of the epitope that can affect accessibility of the antibody to the epitope at a cellular or molecular level, direct inhibition of target antigen activity, conservation of function despite escape mutations, and even non-competitive inhibition sites. (preprints.org)
  • Through the incorporation of novel computational methods for predicting antigen changes to model-informed drug discovery and development, superior vaccines and antibody-based therapeutics or diagnostics can now be more easily designed to mitigate failures. (preprints.org)
  • The screening procedure is performed by directly magnetically labeling the cells with the monoclonal antibody against the specific antigen, conjugated to R-PE and Anti-R-PE magnetic beads, then the labeled cells are subsequently retained to a magnetic column, and the target cells are released and collected for further flow cytometric analysis. (celltechgen.com)
  • An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
  • Immunophenotyping of hWMSCs by flow cytometry To confirm the derived MSCs, specific cell-surface antigens including CD45, CD44, CD34 and CD105 (Sigma, Germany) were probed using monoclonal antibodies and compared with cells treated with control isotype antibodies. (bioskinrevive.com)
  • Flow cytometry analysis of CD19 was performed by staining Raji CD19 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82, yellow histogram) or 0.25 µg CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ (Product # 12-0199-42, blue histogram). (thermofisher.com)
  • Dashed histograms indicate staining with isotype-matched control antibody and solid histograms denote the specific expression of each indicated marker. (bio-rad-antibodies.com)
  • CC Monoclonal antibody isotype: IgG2a. (cellosaurus.org)
  • CD44 is an 80-95 kDa type I transmembrane glycoprotein, also known as Phagocytic glycoprotein-1 (Pgp-1), or Extracellular matrix receptor type III (ECMR-III). (bdbiosciences.com)
  • Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. (thermofisher.com)
  • CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. (biolegend.com)
  • CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. (wdv.com)
  • CD44 is a transmembranous glycoprotein (80 kDa) responsible for cell adhesion that is expressed in a wide range of normal tissues, such as tonsil, breast, prostate, skin, bladder and cervical squamous epithelium (1). (biocare.net)
  • CD44 is a transmembrane glycoprotein that binds to hyaluronic acid, performs roles in various mobile processes and is expressed in quite a lot of cell sorts. (citologica.org)
  • 3. Endo K., Terada T. Protein expression of CD44 (standard and variant isoforms) in hepatocellular carcinoma: relationships with tumor grade, clinicopathologic parameters, p53 expression, and patient survival. (roche.com)
  • CD44 variant isoforms are also relevant to the progression of head and neck squamous cell carcinoma. (wikipedia.org)
  • CD44 is a transmembrane molecule appearing in numerous isoforms generated by insertions of alternatively spliced variant exons ( CD44v ) and having various binding partners. (nature.com)
  • However, numerous further isoforms of CD44 are generated by insertions of different variable regions into a single site of its extracellular domain. (nature.com)
  • In contrast to CD44s, the CD44 isoforms containing variable regions are expressed only in distinct epithelial cell types (e.g., keratinocytes), activated immune cells, and several types of cancers. (nature.com)
  • There is no known non-specific antibody reactivity observed in this product. (roche.com)
  • Reactivity for CD44 was restricted to progenitor cells giving rise to the nucleus pulposus (NP) of the developing IVDs. (bvsalud.org)
  • Subsequently, by means of flow cytometry, these cells were quantified and presented positive results for both markers, CD271 (0.6) and CD44 (95.7 + -3.8) in 10,000 cells. (umich.edu)
  • Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD48 (MEM-102) purified antibody (concentration in sample 3 μg/ml, GAM APC). (exbio.cz)
  • Separation of CD48 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD48 (MEM-102) purified antibody (concentration in sample 3 μg/ml, GAM APC). (exbio.cz)
  • CD44 percentage values in peripheral T-lymphocytes were significantly higher in patients than controls, as detected by flow cytometry. (who.int)
  • Check out our catalog of ~700 optimized Flow Cytometry antibodies available in a "100 tests" size and designed to deliver brilliant results in your experiments. (biossusa.com)
  • Results: The isolated cells showed high expression of CD44 (98.8%), CD73 (100%), and CD90 (97.2%), and moderate expression of STRO-1 (18.4%) and ALDH1 (16.2%), by flow cytometry. (bvsalud.org)
  • 1983) showed that the antigens defined by monoclonal antibodies A3D8 and A1G3 are determined by genes on 11p. (wdv.com)
  • We aimed to determine the significance of detecting expression of some antigens that might be pivotal to the process, namely CD44 and CD117, in patients with active pemphigus vulgaris. (who.int)
  • CD44 is a receptor for hyaluronic acid and internalizes metals bound to hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). (wikipedia.org)
  • CD44 is the receptor for hyaluronic acid. (bdbiosciences.com)
  • The 515 monoclonal antibody can reportedly block cellular adhesion to hyaluronic acid. (bdbiosciences.com)
  • Naor D. Editorial: Interaction between hyaluronic acid and its receptors (CD44, RHAMM) regulates the activity of inflammation and cancer. (libguides.com)
  • 1,2,3,4 CD44 is one of two major hyaluronan (HA) binding proteins required for activation of hyaluronan. (roche.com)
  • 1,2 The extensive glycosylation patterns of CD44 are important for the recognition and binding of the CD44-hyaluronan complex. (roche.com)
  • 5. Bartolazzi A., Nocks A., et al.Glycosylation of CD44 is implicated in CD44-mediated cell adhesion to hyaluronan. (roche.com)
  • CD44 is a member of the hyaladherin family of hyaluronan-binding proteins, with structural similarities to selectins. (bdbiosciences.com)
  • CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility. (libguides.com)
  • Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. (thermofisher.com)
  • ProductData": { "ID": "RTD000659", "ProductType": "Others", "BrandName": "anti-CD44 (SP37) Rabbit Monoclonal Primary Antibody", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For in vitro diagnostic use. (roche.com)
  • Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Content", "Value": "Anti-CD44 (SP37) Rabbit Monoclonal Primary Antibody contains sufficient reagent for 50 tests. (roche.com)
  • One 5 mL dispenser of anti-CD44 (SP37) contains approximately 3.5 μg of a rabbit monoclonal antibody. (roche.com)
  • Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "L'anticorps Anti-CD44 (SP37) Rabbit Monoclonal Primary Antibody est dirigé contre la protéine CD44. (roche.com)
  • Cet anticorps est conçu pour être utilisé en diagnostic in vitro (DIV)", "Language": "fr", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "Anti-CD44 (SP37) is a rabbit monoclonal antibody produced against a conserved region of the CD44 protein. (roche.com)
  • Tissue samples were immunostained with a commercial anti-human CD44 rabbit monoclonal antibody at 1:100 dilution. (bvsalud.org)
  • The Anti Flag Antibody Mouse Monoclonal reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (crossfirebeilstein.com)
  • Bead release reagent and Stop Buffer can be used to release the cell with antibody-R-PE from magnetic beads. (celltechgen.com)
  • Nuclear staining was also present, as was staining of intracellular and extracellular vesicles with ISL1 antibody. (bvsalud.org)
  • The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. (thermofisher.com)
  • RESULTS: In contrast to normal oesophagus, which showed positivity for both CD44 epitopes (CD44H and CD44v3) in the basal third of the epithelium, antral and intestinal subtypes of Barrett oesophagus expressed CD44H only, the distribution being focal in non-dysplastic and diffuse in dysplastic Barrett mucosa. (bmj.com)
  • For several reasons there is a growing interest in the scientific community in immunoglobulin Y as antibiotic alternative and their oral administration in the polyclonal antibody (pAb) format, to maintain animal health and performance, do not require IgY purification for large-scale production, resulting in protein impurities and high concentration of biotin in the samples. (preprints.org)
  • CD44 is a multistructural and multifunctional cell surface molecule involved in cell proliferation, cell differentiation, cell migration, angiogenesis, presentation of cytokines, chemokines, and growth factors to the corresponding receptors, and docking of proteases at the cell membrane, as well as in signaling for cell survival. (wikipedia.org)
  • Treatment of LMP1/CD40-expressing lymphomatous mice with an anti-PD-L1 monoclonal antibody induced tumor regression with decreased spleen content, activation and proliferation rate of B-cells as well as a marked increase in T-cell activation, as assessed by CD62L and CD44 expression. (springer.com)
  • Multiple myeloma (MM) is a hematological malignancy characterized by the proliferation of transformed monoclonal plasma cells in the bone marrow (BM) [ 1 ]. (biomedcentral.com)
  • Vermot-Desroches C, Wijdenes J, Valmu L , Roy C, Pigott R, Nortamo P and Gahmberg C G (1995) A CD44 monoclonal antibody differentially regulates CD11a/CD18 binding to intercellular adhesion molecules CD54, CD102 and CD50. (leenavalmu.fi)
  • The antibody is diluted in Tris-HCl dilution buffer with Brij-35, 1% carrier protein, and 0.1% ProClin 300, a preservative. (roche.com)
  • Primary dye-conjugated antibodies should be titrated to determine the optimal staining dilution. (celltechgen.com)
  • 4. Southgate J., Trejdosiewicz L. et al.Patterns of splice variant CD44 expression by normal human urothelium in situ and in vitro and by bladder-carcinoma cell lines. (roche.com)
  • Ex vivo glycan engineering of the surface of live cells has been used to enforce HCELL expression on any cell that expresses CD44. (wikipedia.org)
  • CD44 expression is an indicative marker for effector-memory T-cells. (wikipedia.org)
  • Endometrial cells in women with endometriosis demonstrate increased expression of splice variants of CD44, and increased adherence to peritoneal cells. (wikipedia.org)
  • In many cancers (renal cancer and non-Hodgkin's lymphomas are exceptions), a high level of CD44 expression is not always associated with an unfavorable outcome. (wikipedia.org)
  • As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. (biolegend.com)
  • High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. (biolegend.com)
  • The immunohistochemical results showed prominent expression of CD44 in 13 of the patients and CD117 in 9 of the patients with new blister formation. (who.int)
  • In breast cancer studies, evaluation of CD44 expression has been useful in the differentiation of benign and malignant papillary lesions (2). (biocare.net)
  • Expression of CD44s, the most common CD44 isoform, has been reported to be associated with increased survival of patients with nodenegative invasive breast carcinoma (3). (biocare.net)
  • Overall, B-DCs demonstrated higher expression of CD86 and CD172α than L-DCs, but both cell types expressed high levels of MHC class II and CD44, as well as moderate amounts of CD163, CD204, and Bla36. (biomedcentral.com)
  • CD44 expression was always restricted to IVD precursor cells, whereas cartilage precursors were devoid of labelling. (bvsalud.org)
  • CD44 splice variants containing variable exons are designated CD44v. (wikipedia.org)
  • Splice variants of CD44 on colon cancer cells display sialofucosylated HCELL glycoforms that serve as P-, L-, and E-selectin ligands and fibrin, but not fibrinogen, receptors under hemodynamic flow conditions pertinent to the process of cancer metastasis. (wikipedia.org)
  • CD44 Splice Variants as Potential Players in Alzheimer's Disease Pathology. (libguides.com)
  • 2,3,4 There are 10 variants of CD44 due to differential splicing. (roche.com)
  • Monoclonal antibodies against CD44 variants include bivatuzumab for v6. (wikipedia.org)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • Specific antibody concentration is approximately 0.7 μg/mL. (roche.com)
  • 5 The CD44 molecule is known to present several different glycosylation, oligomerization, and protein sequence patterns due to differential mRNA splicing. (roche.com)
  • High levels of the adhesion molecule CD44 on leukemic cells are essential to generate leukemia. (wikipedia.org)
  • CD44 is a transmembrane molecule with multiple, in part poorly understood functions. (nature.com)
  • CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. (biolegend.com)
  • 1. Aruffo A, Stamenkovic I, et al.CD44 is the principal cell surface receptor for hyaluronate. (roche.com)
  • 1990) demonstrated that CD44 is the main cell surface receptor for hyaluronate. (wdv.com)
  • The mouse monoclonal antibody Hermes-3 recognizes the 85-95 kD human lymphocyte homing receptor. (wdv.com)
  • 25 the CD44 cell surface receptor. (hifsignaling.com)
  • Rigorous verification of this antibody is conducted to ensure its accuracy, and it can be utilized for detecting human JUN protein in ELISA, WB, and IHC experiments. (cusabio.com)
  • CD44 is expressed in many normal human hematopoietic, epithelial,and mesenchymal cells. (roche.com)
  • Flow cytometric analysis of CD44 on human peripheral blood lymphocytes. (bdbiosciences.com)
  • Find CD44 Antibodies for a variety of species such as anti-Human CD44, anti-Mouse CD44, anti-Rat CD44. (antibodies-online.com)
  • Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • Human IgG antibody Laboratories manufactures the anti flag antibody mouse monoclonal reagents distributed by Genprice. (crossfirebeilstein.com)
  • Description: A Monoclonal antibody against Human Flag. (crossfirebeilstein.com)
  • Applications Tested: The WM-59 (WM59) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • Studies have shown that CD44, also known as HCAM, plays an important role in the cascade of metastasis and progression of human malignant tumors (2). (biocare.net)
  • Hallikas O K, Aaltonen J M, von Koskull H, Lindberg L A, Valmu L , Kalkkinen N, Wahlström T, Kataoka H, Andersson L, Lindholm D and Schröder J (2006) Identification of antibodies against HAI-1 and integrin α6β4 as immunohistochemical markers of human villous cytotrophoblast. (leenavalmu.fi)
  • Experiments in animals have shown that targeting of CD44 by antibodies, antisense oligonucleotides, and CD44-soluble proteins markedly reduces the malignant activities of various neoplasms, stressing the therapeutic potential of anti-CD44 agents. (wikipedia.org)
  • Abcam: antibodies, proteins, kits. (abcam.com)
  • The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions. (biolegend.com)
  • The following product was used in this experiment: CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ from Thermo Fisher Scientific, catalog # 12-0199-42, RRID AB_1834376. (thermofisher.com)
  • The following product was used in this experiment: CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Functional Grade, eBioscience™ from Thermo Fisher Scientific, catalog # 16-0319-82, RRID AB_468934. (thermofisher.com)
  • Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Reported: The WM-59 (WM59) antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis . (biolegend.com)
  • First, the cells are labeled by Rat CD44 monoclonal antibody conjugated to R-PE and anti-R-PE magnetic beads, the Rat CD44 + cells are positively selected on a magnetic column, are collected for further flow-cytometric analysis. (celltechgen.com)
  • CD44 glycosylation also directly controls its binding capacity to fibrin and immobilized fibrinogen. (wikipedia.org)
  • CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. (wikipedia.org)
  • Furthermore, because alternative splicing and posttranslational modifications generate many different CD44 sequences, including, perhaps, tumor-specific sequences, the production of anti-CD44 tumor-specific agents may be a realistic therapeutic approach. (wikipedia.org)
  • The pre-clinical LMP1/CD40-expressing transgenic mouse model is characterized by B-cell specific CD40 signaling responsible for NF-κB continuous activation with a spleen monoclonal B-cell tumor after 1 year in 60% of cases. (springer.com)
  • Importantly, stimulation of T cells by Cd44v7- deficient macrophages induced upregulation of Foxp3 in vitro , while cotransfer of Cd44 WT macrophages into Cd44v7- deficient mice reduced Foxp3 + T-cell counts and caused colitis. (nature.com)
  • Monoclonal antibodies were used for detection of the. (lu.se)
  • Monoclonal antibodies were used for detection of the proteoglycans syndecan-1, -2 and -4, glypican, CD44 and perlecan. (lu.se)
  • Description: Mouse IgG monoclonal antibody for Flag Tag detection. (crossfirebeilstein.com)
  • CONCLUSIONS: Our study shows, for the first time, that the stem cell marker CD44 selectively marks intervertebral disc progenitor cells, paralleling their differentiation toward a discogenic phenotype. (bvsalud.org)
  • In addition, variations in CD44 are reported as cell surface markers for some breast and prostate cancer stem cells. (wikipedia.org)
  • CD44 is expressed on leucocytes, erythrocytes, epithelial cells and weakly on platelets. (bdbiosciences.com)
  • Here we demonstrate that Cd44v7 -deficient T cells - like Cd44 wild-type ( Cd44 WT ) T cells - provoked disease in two different colitis models: the model induced by CD4 + CD45RB high T-cell transfer into Rag2 -deficient mice and a new model based on ovalbumin (OVA)-specific T-cell transfer into Rag -sufficient, OVA-challenged mice. (nature.com)
  • After tissue was disgregated, stem cells were isolated from all other tissue cells using the magnetic separation technique (MiltenyiBiotec), in which CD271 and CD44 monoclonal antibodies were used. (umich.edu)
  • The antibody stained cells (about 0.5 106 cells per petri dishes) order BAY 63-2521 were evaluated by FACS Calibur order BAY 63-2521 flow cytometer (Becton Dickinson, NJ, USA), with at least 10000 events being order BAY 63-2521 analyzed. (bioskinrevive.com)
  • This kit is developed to isolate and detect the Rat CD44 + cells using magnetically labeled with Magnetic beads. (celltechgen.com)
  • 5) Wash cells to remove unbound primary antibody by adding 1−2 mL of buffer per 10 7 cells and centrifuge at 300×g for 10 minutes. (celltechgen.com)
  • The obtained JUN antibody gene is inserted into a vector and transfected into host cells for culture. (cusabio.com)
  • Anti-T lymphocyte globulin (ATG) induces generation of regulatory T cells, at least part of them express activated CD44. (libguides.com)
  • CD44 is a kind I transmembrane protein expressed in varied sorts of regular most cancers cells, together with pancreatic, breast, and oral cancers. (inacj.com)
  • Development and characterization of new monoclonal antibodies against porcine Interleukin-17A and Interferon-gamma. (usda.gov)
  • On the contrary, in some neoplasms CD44 upregulation is associated with a favorable outcome. (wikipedia.org)
  • Multiple myeloma (MM) is the second most common hematologic malignancy and remains incurable despite the advent of numerous new drugs such as proteasome inhibitors (PIs), immunomodulatory agents (IMiDs), and monoclonal antibodies. (biomedcentral.com)
  • Functionally, CD44 is involved in lymphocyte recirculation, adhesion, activation, lymph node homing, and to a larger extent in other tissue types, cell- cell and cell-matrix interactions. (roche.com)