Monoclonal antibody monocytes. Medical search. Frequent questions

Our study examined if nonpathogenic bacteria or lipopolysaccharide (LPS), can affect epithelial function in the presence of monocytes/macrophages. (nih.gov)
The MoP9 antibody reacts with CD14, a 53 - 55 kDa glycosylphosphatidylinositol (GPI)-anchored transmembrane glycoprotein expressed at high levels on the surface of peripheral blood monocytes and macrophages, and at lower levels on granulocytes. (stemcell.com)
An approximate 10-fold difference in expression levels between monocytes/macrophages and granulocytes makes CD14 a useful marker for distinguishing these cell populations. (stemcell.com)
Atherosclerosis starts with dysfunctional changes in the endothelium induced by disturbed shear stress which can lead to endothelial and platelet activation, adhesion of monocytes on the activated endothelium, and differentiation into proinflammatory macrophages, which increase the uptake of oxidized LDL (oxLDL) and turn into foam cells, exacerbating the inflammatory signalling. (hindawi.com)
A greater number of alveolar macrophages produced TNF-α compared to autologous monocytes in sarcoidosis but not in controls and, based on cell size, it was confirmed that this was the product of more mature macrophages and that the secretion of TNF-α by monocytes and macrophages was heterogeneous: not all monocytes and macrophages secrete TNF-α. (ersjournals.com)
No differences in the average levels of TNF-α secretion by peripheral blood monocytes or alveolar macrophages were observed. (ersjournals.com)
C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. (origene.com)
Monoclonal anti Fc receptor IgG blocks enhancement of viral replication in macrophages by anti-viral antibody. (microbiologyresearch.org)
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. (biolegend.com)
It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. (novusbio.com)
These findings suggest that the MCP-1 production in IPF and nonIPF plays an important role in the recruitment of monocyte/macrophages. (elsevierpure.com)
CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55). (lsbio.com)
CD52 is expressed on lymphocytes, monocytes, macrophages and on epithelial cells of male reproductive tract, shed into seminal plasma and acquired by spermatozoa. (beckman.com)

We observed that age advancement in all three groups combined was associated with a monocyte immune phenotypic profile related to inflammation and a T cell immune phenotypic associated with immune senescence and chronic antigen exposure. (frontiersin.org)
The identified monocyte and T cell immune phenotypic profiles that were associated with age advancement, were strongly related to inflammation, chronic antigen exposure and immune senescence. (frontiersin.org)
This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. (frontiersin.org)
METHODS: Single and double immunoenzymatic staining procedures, immunoperoxidase, and immunoalkaline phosphatase were used with Ki-67, a monoclonal antibody that recognises a nuclear antigen present in proliferating cells, with markers expressed in B and T lymphocytes and monocytes. (bmj.com)
See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more. (lsbio.com)
The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. (origene.com)
It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC). (biolegend.com)
This monoclonal antibody reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. (novusbio.com)
The same antibody molecule can cross-react with related antigens if their epitopes are similar enough to those of the original antigen. (msdmanuals.com)
Such chronic injury is usually mediated by anti-human leukocyte antigen antibodies. (medscape.com)

An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
As positive control a polyclonal anti-C1q antibody was used and as negative control an isotype matched monoclonal antibody (Ref.1). (origene.com)
Human peripheral blood monocytes were stained with Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227, filled histogram) or isotype control antibody (Catalog # MAB003 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab') 2 Secondary Antibody (Catalog # F0101B ). (rndsystems.com)
Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c PE-conjugated Monoclonal Antibody (Catalog # FAB1777P ) and either (A) Mouse Anti-Human BATF3 Monoclonal Antibody (Catalog # MAB7437) or (B) Mouse IgG 2B Flow Cytometry Isotype Control (Catalog # MAB0041 ) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B ). (rndsystems.com)
Human peripheral blood monocytes were stained with CD64 (clone 10.1) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram). (biolegend.com)

The goal of this study was to determine the contribution of monocyte/macrophage ABCA1 to HDL formation in vivo. (jci.org)
Using the reverse haemolytic plaque assay, the aims of this study were to determine if the upregulation of TNF-α could be confirmed and to investigate whether this was monocyte or macrophage specific. (ersjournals.com)
Mouse monoclonal antibody raised against macrophage/monocyte. (abnova.com)
Both monoclonal antibodies could enhance virus infectivity of Fc receptor-bearing macrophage-like cells when present at suitable dilutions. (microbiologyresearch.org)
Antibody-mediated enhancement of flavivirus replication in macrophage-like cell lines. (microbiologyresearch.org)
To examine the mechanisms for increased monocyte/macrophage recruitment in IPF and nonIPF interstitial lung diseases (nonIPF) the localization of monocyte chemoattractant protein-1 (MCP-1) was investigated in 14 cases of IPF, seven cases of nonIPF, and seven normal control lungs (CTRL) by immunohistochemistry using a specific anti-MCP-1 monoclonal antibody, F9. (elsevierpure.com)
Blood monocyte-derived DCs (B-DCs), as one group of myeloid DCs, can be generated by incubation of monocytes that are isolated from PBMCs with exogenous granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 6-7 days [ 3 ]. (biomedcentral.com)

We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)
The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88. (lsbio.com)

Monoclonals developed from these tumor antigens are in the initial phases of investigation with regard to their specificity and antitumor activity. (jcancer.org)

Lamina propria mononuclear cells (LPMCs) and peripheral blood monocytes (PBMs) were obtained from patients with Crohn's disease and control patients. (nih.gov)
Characterisation of normal peripheral blood cells in cycle identified by monoclonal antibody Ki-67. (bmj.com)
Antibody-dependent cell-mediated cytotoxicity (ADCC) was evaluated with FRβ positive CHO cells as target cells and isolated peripheral blood monocytes as effector cells in an in vitro assay. (biomedcentral.com)
Detection of CXCR7/RDC‑1 in Human peripheral blood Monocytes by Flow Cytometry. (rndsystems.com)
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD244 (2-69) APC antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Separation of human CD123 positive CD244 positive basophils (red-filled) from CD244 negative CD123 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD244 (2-69) APC antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Lee HR, Son CH, Koh EK, Bae JH, Kang CD, Yang K, Park YS: Expansion of cytotoxic natural killer cells using irradiated autologous peripheral blood mononuclear cells and anti-CD16 antibody. (exbio.cz)
We found that bronchoalveolar lavage fluid (BALF) from 11 patients with idiopathic pulmonary alveolar proteinosis (IPAP) suppressed the growth of peripheral blood monocytes and TF-1 cells, a cell line dependent on either GM-CSF or interleukin-3 (IL-3). (wiley.com)
Previous studies have suggested that specific IGF-I receptors are present on peripheral blood leukocytes, including polymorphonuclear leukocytes, lymphocytes, and monocytes. (utmb.edu)
We conclude from these data that among human peripheral blood nonactivated mononuclear cells, IGF-I binds to specific type I IGF receptors found predominately on monocytes and B-lymphocytes. (utmb.edu)
Our results show that the murine thymoma cell line EL-4 is superior to peripheral blood mononuclear cells in both cellular outgrowth and cloning experiments, whereas monocyte-derived factors and monocyte cell lines were less capable than peripheral blood mononuclear cells in enhancing cellular outgrowth and cloning. (lu.se)

We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
Monoclonal antibodies developed against immunogenic proteins (Tumor Specific Antigens/TSA's) that are expressed in human cancers, display a unique behavioral pattern. (jcancer.org)
Two color flow cytometric analysis of mononuclear cells stained with mouse monoclonal antibodies specific for the human IGF-I receptor, the human insulin receptor, and monoclonal antibodies directed against specific monocyte and lymphocyte subset cell surface antigens revealed that both IGF-I receptors and insulin receptors were present on nearly all monocytes and B-lymphocytes, but were present on only 2% of T-lymphocytes. (utmb.edu)
Antibodies recognize specific configurations (epitopes, or antigenic determinants) on the surfaces of antigens (eg, proteins, polysaccharides, nucleic acids). (msdmanuals.com)
Antibodies and antigens fit tightly together because their shape and other surface properties (eg, charge) are complementary. (msdmanuals.com)

LFA-1 participates in several processes, such as T cell-mediated killing, T helper and B lymphocyte responses, natural killing, Ab-dependent cellular cytotoxicity by monocytes and granulocytes, and adherence of leukocytes to endothelial cells, fibroblasts, and epithelial cells. (sigmaaldrich.com)
CD97 is highly expressed on monocytes and granulocytes. (bdbiosciences.com)

The VIM3b monoclonal antibody recognizes CD97. (bdbiosciences.com)
The antibody recognizes proteins of 74, 80 and 89 kDa. (bdbiosciences.com)
Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. (fishersci.com)
The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q. (origene.com)
The mouse monoclonal antibody 2-69 recognizes an extracellular epitope of CD244, a transmembrane glycoprotein expressed on NK cells, basophils, monocytes, an some T cell subsets. (exbio.cz)

The following product was used in this experiment: S100A9 Monoclonal Antibody (OTI3C12), TrueMAB™ from Thermo Fisher Scientific, catalog # TA804270. (thermofisher.com)
The following product was used in this experiment: LILRB3 Monoclonal Antibody (OTI3G9), TrueMAB™ from Thermo Fisher Scientific, catalog # TA812163. (thermofisher.com)
CXCR7/RDC-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001 ). (rndsystems.com)

Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)

After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. (lsbio.com)

A study, led by Cambridge University, has found alemtuzumab, a humanized monoclonal antibody that targets CD52 on lymphocytes and monocytes designed to treat a form of leukaemia , not only stops MS from advancing in patients with relapsing-remitting multiple sclerosis (RRMS) but may also restore lost function. (disabled-world.com)

These age-related immunological changes resemble those seen during treated HIV-1 infection and include high levels of soluble inflammatory proteins, high levels of monocyte and T cell activation, T cell exhaustion and senescence, and low levels of naïve T cells ( 14 - 25 ). (frontiersin.org)
The monoclonals (mAbs) that we have developed against specific immunogenic tumor membrane proteins have been studied in detail. (jcancer.org)
Monoclonals that we were able to develop from tumor specific proteins derived from colon and pancreas cancer were found capable of targeting those tumors to induce apoptosis. (jcancer.org)

Recombinant goat monoclonal antibody raised against rabbit IgG Fab. (abnova.com)
Recombinant goat monoclonal antibody raised against rabbit IgG Fc. (abnova.com)
Formalin-fixed, paraffin-embedded human tonsil stained with CD14 Rabbit Recombinant Monoclonal Antibody (LPSR/4180R). (neobiotechnologies.com)

In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. (rndsystems.com)
The antibody MEM-143 reacts with a protein determinant of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). (lsbio.com)

It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. (fishersci.com)
CD62L is expressed on neutrophils, monocytes, T- and B-lymphocyte subsets and NK cells. (bdbiosciences.com)

Defective migration of monocyte-derived dendritic cells in LAD-1 immunodeficiency. (lu.se)

He was also involved in the development of mouse monoclonal antibodies directed against human tissues that are widely used as diagnostic reagents. (wikipedia.org)
Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. (fishersci.com)
TNF Alpha antibody LS-B2123 is an unconjugated mouse monoclonal antibody to TNF Alpha from human. (lsbio.com)
The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. (origene.com)
Mouse monoclonal antibody raised against full length native of rabbit troponin T. (abnova.com)
Mouse monoclonal antibody raised against native myosin. (abnova.com)
A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. (microbiologyresearch.org)
Rapid development of neutralizing and diagnostic SARS-COV-2 mouse monoclonal antibodies. (cdc.gov)

secondary sites are monocytes, fibroblasts and glial cells. (enzolifesciences.com)
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. (biolegend.com)
Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. (biolegend.com)
Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
Elotuzumab (Elo) is an IgG 1 monoclonal antibody targeting SLAMF7 (CS1, CRACC, and CD319), which is highly expressed on multiple myeloma (MM) cells, natural killer (NK) cells, and subsets of other leukocytes. (aacrjournals.org)
Monocyte chemoattractant protein-1 production by epithelial cells in IPF may be caused by the metaplastic nature of the epithelial cells and may be one of the key factors inducing the irreversible progression of IPF. (elsevierpure.com)
Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells. (cdc.gov)
However, because of low transformability, low clonability, and the inherent instability of EBV-infected B cells, valuable antibody-producing B cells are often lost during this procedure. (lu.se)

Antibody-dependent phagocytosis is an immune mechanism enhanced by opsonization, where typically, more bound antibodies trigger a stronger phagocyte response. (lu.se)
Here, we show that Spike-specific antibodies, dependent on concentration, can either enhance or reduce Spike-bead phagocytosis by monocytes independently of the antibody neutralization potential. (lu.se)
These results suggest that the levels of anti-Spike antibodies could influence monocyte-mediated immune functions and propose that non-neutralizing antibodies could confer protection to SARS-CoV-2 infection by mediating phagocytosis. (lu.se)

Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis. (fishersci.com)
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. (biolegend.com)

Furthermore, CD38 ligation by specific MoAb reduced the IFN-gamma-dependent enhancement of monocyte-dynamic adhesion to endothelial monolayers. (nih.gov)

Salvage therapy for patients with resistant disease or relapse includes reuse or alternative use of front-line agent, combination therapy, thalidomide (with or without steroids), autologous transplantation, and monoclonal antibody (alemtuzumab). (medscape.com)

His contributions to immunology have focused on the molecular features of antibody variable regions, including key discoveries related to the antibody combining site and its relationship to antibody idiotypy and the hypervariable regions of antibody molecules. (wikipedia.org)

article{014d014e-4a07-4d4f-9d94-15d649e06b3c, abstract = {{Spike-specific antibodies are central to effective COVID19 immunity. (lu.se)

These findings identify IFN-gamma as a modulator of monocytic CD38 expression and indicate that CD38 plays a specific role in the activation and adhesion processes performed by monocytes. (nih.gov)

Milestones in technological advancements in immunoglobulin production processes, such as development of new plasma purification methods and more effective antibodies, have offered robust backings to. (reportlinker.com)

The therapeutic landscape has witnessed a transformative shift with the dominating presence of monoclonal antibodies (mAbs). (reportlinker.com)
The biopharmaceutical industry is entering an era dominated by sophisticated therapeutics, and Bi-Specific Monoclonal Antibodies (MAbS) represent a cutting edge development in this sphere. (reportlinker.com)

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. (novusbio.com)
Surprisingly, we find that both convalescent patient plasma and patient-derived monoclonal antibodies lead to maximum opsonization already at low levels of bound antibodies and is reduced as antibody binding to Spike protein increases. (lu.se)

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)

This 1RNM44N antibody will recognize a formaldehyde-fixed epitope. (fishersci.com)

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions. (biolegend.com)

Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Human Spleen tissue lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
Although advances in tissue typing and cross-match testing have helped to deter severe transplant rejection, rejection may result from direct cellular attack (cellular rejection) or be mediated by antibodies (humoral rejection). (medscape.com)

Physiological role of CD52 on lymphocytes is unclear, although CAMPATH-1 antibody is capable of complement activation and it has been used in lympho proliferative disorders. (beckman.com)

In this study we aimed to develop an FRβ-specific human monoclonal antibody (mAb) that could be used as a therapeutic agent to treat rheumatoid arthritis and other autoimmune diseases, as well as FRβ positive cancers. (biomedcentral.com)

Biopharmaceuticals, particularly therapeutic antibodies, are revolutionizing modern medical treatments due to their targeted approach and ability to harness the body's own immune responses. (reportlinker.com)
He also co-authored a series of papers which utilized human plasmablasts after immunization to define the human immune response to influenza vaccine and generated human monoclonal antibodies from the blood. (wikipedia.org)
Interestingly, a unique monocyte and T cell immune phenotypic profile predictive for age advancement was found within each group. (frontiersin.org)
An inflammatory monocyte immune phenotypic profile associated with age advancement in HIV-negative individuals, while the monocyte profile in blood donors and people with HIV was more reflective of loss of function. (frontiersin.org)
While the monocyte and T cell immune phenotypic profile within the HIV-negative individuals reflected those observed in the combined three groups, a distinct profile related to immune dysfunction, was observed within blood donors and people with HIV. (frontiersin.org)

Accordingly, the CD44v7 ligand osteopontin, whose levels were elevated in Crohn's disease, specifically induced IL-6 in human monocytes, a cytokine also increased in these patients. (nature.com)

The increasing prevalence of neurodegenerative diseases and the mounting demand for effective therapeutic options propel interest in the neuroscience antibodies and assays segment. (reportlinker.com)
Functional assays (Ref.1): Antibody JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. (origene.com)

The DREG-56 monoclonal antibody specifically binds to CD62L. (bdbiosciences.com)

Anatomy29

Organisms13

Diseases11

Chemicals and Drugs114

Analytical, Diagnostic and Therapeutic Techniques and Equipment48

Phenomena and Processes44

Disciplines and Occupations3

Information Science3

Health Care2

Macrophages13

Antigen10

Isotype5

Macrophage7

Reactivity3

Specificity1

Peripheral11

Antigens6

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Recognizes5

Catalog3

Immunohistochemistry1

Secondary antibody1

Lymphocytes and monocytes1

Proteins3

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Reacts2

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Mouse8

Cells8

Phagocytosis3

Flow cytometric analysis2

Endothelial1

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Immunology1

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Adhesion1

Immunoglobulin1

MAbS2

Protein2

Invitrogen1

Epitope1

Affinity chromatography2

Tissue2

CD521

Rheumatoid1

Immune5

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Assays2

Binds1