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Receptors, LamininLamininAntibodies, MonoclonalAntibodiesAntibody SpecificityIntegrin alpha6beta1EpitopesIntegrinsAntibodies, ViralIntegrin alpha6Fluorescent Antibody TechniqueBasement MembraneCell AdhesionMolecular Sequence DataAntibodies, BacterialAmino Acid SequenceReceptors, ImmunologicDystroglycansMice, Inbred BALB CMolecular WeightCross ReactionsHybridomasCell LineFibronectinsIntegrin alpha6beta4Antibodies, NeutralizingAntibody AffinityBinding Sites, AntibodyReceptors, AntigenAntibody FormationIntegrin alpha3beta1ImmunohistochemistryAntigens, SurfaceCells, CulturedDual Specificity Phosphatase 2Epitope MappingLutheran Blood-Group SystemAntibodies, Anti-IdiotypicEnzyme-Linked Immunosorbent AssayAntigens, CD29Neutralization TestsTumor Cells, CulturedChromatography, AffinityImmunoenzyme TechniquesExtracellular MatrixAntibodies, NeoplasmBlotting, WesternImmunoglobulin Fab FragmentsImmunoglobulin GMatrix Metalloproteinase 11Base SequencePeptide FragmentsProtein BindingCollagenAntigen-Antibody ReactionsCatechinAntigens, NeoplasmAntigens, CDAntibodies, Monoclonal, HumanizedIntegrin alpha3Electrophoresis, Polyacrylamide GelAntigen-Antibody ComplexFlow CytometryHIV AntibodiesGlycoproteinsImmune SeraRNA, MessengerImmunoglobulin MRecombinant ProteinsImmunoblottingCell MovementRabbitsBinding, CompetitiveImmunologic TechniquesIntegrin alpha1beta1Antibodies, ProtozoanMembrane GlycoproteinsImmunosorbent TechniquesRadioimmunoassayImmunization, PassiveIntegrin alpha ChainsAntigens, BacterialAntigens, ViralBinding SitesAntibodies, FungalFluorescent Antibody Technique, IndirectAntigensAntibodies, AntinuclearCell Adhesion MoleculesCell MembraneMice, Inbred C57BLAutoantibodiesProtein PrecursorsT-LymphocytesOligopeptidesCloning, MolecularMicroscopy, ElectronMelanomaTissue DistributionIntegrin alpha2
Primary AntibodiesIntegrinCloneMAbsAntigenSubunitSkeletal muscleAnti-lamininFibronectin and lamininProteinsPolyclonal antibodyProteinBindsTyrosineRecombinantIsotype ControlWestern blotSecondary AntibodiesImmunoglobulinsMembraneTransmembrane receptorBoundGeneInhibitorsMouseIntegrinsAssaysSubunitsAffinity chromatographyAntigensReagentsPostsynapticInhibitoryMoleculeMembranesEstrogenGlomeruliAgonistEpidermalExtracellularHumanCatalogActinMolecularAlphaTherapeuticTumorTissueBasalStainMechanism
Primary Antibodies1
Integrin4
  • Integrin alpha 6 beta 1 is a receptor for laminin. (rndsystems.com)
  • Integrin alpha 6 beta 4 is also a receptor for laminin and is a structural component of hemidesmosomes in stratified epithelia. (rndsystems.com)
  • Be the first to review Human Integrin alpha 6/CD49f Alexa Fluor® 405-conjugated Antibody and earn rewards! (rndsystems.com)
  • The Integrin alpha 2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with the Integrin alpha 1 (CD29) or alpha 4 (CD104) subunits and binds Laminin. (rndsystems.com)
Clone1
  • Mouse anti Human CD239 antibody, clone BRIC221 ( MCA1982 ) used to look for blocking of Lu-Fc to endogenous α5 laminin in kidney sections. (bio-rad-antibodies.com)
MAbs4
  • Purified mAbs are >90% specific antibody. (antibodiesinc.com)
  • Selective compounds have been developed that target either the extracellular ligand-binding region of the EGFR (including a number of monoclonal antibodies [MAbs], immunotoxins, and ligand-binding cytotoxic agents) or the intracellular tyrosine kinase region (including various small-molecule inhibitors). (medscape.com)
  • Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)
  • We have used monoclonal antibodies (mAbs) as probes to study the dependence of the structure of different regions of the alphaM subunit on association with the beta2 subunit in the alphaM/beta2 heterodimer. (embl.de)
Antigen6
  • This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. (frontiersin.org)
  • These observations establish laminin-332 as a novel basement membrane antigen in dogs with autoimmune blistering diseases with variable clinical phenotypes. (morulaivf.com)
  • This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • The invention relates to methods of modulating the antigen-binding affinity of an antibody by determining, using data corresponding to the structure of a complex between the antibody and an antigen in a solvent, a representation of a charge distribution of the CDRs of the antibody which minimizes electrostatic contribution to binding free energy between the antibody and the antigen in a solvent. (justia.com)
  • Guided by these determinations, the antibody is accordingly modified (altered) to improve upon, e.g., antibody/antigen binding by modifying at least one amino acid residue to decrease the binding free energy between the antibody and antigen when bound in a solvent. (justia.com)
  • Researchers hoped that such antibodies would be no more foreign to the human body than a genuine human antibody, but the utility of such antibodies has been restricted, at least in some cases, by a reduction in the antibody's affinity for the antigen. (justia.com)
Subunit1
  • The activated receptor catalyzes removal of GDP from the G protein alpha subunit (Gα i ), which allows intracellular GTP to bind. (elifesciences.org)
Skeletal muscle6
  • A number of studies have previously proposed the existence of glucocorticoid receptors on the plasma membrane of many cell types including skeletal muscle fibres. (uea.ac.uk)
  • In this study, we investigated the localisation and the mechanism(s) underlying the non-genomic physiological functions of these receptors in mouse skeletal muscle cells. (uea.ac.uk)
  • From these results we conclude that the non-genomic/non-canonical physiological functions of glucocorticoids, in adult skeletal muscle fibres are mediated by a glucocorticoid receptor localised in the extracellular matrix, in satellite cells and close to mitochondria and involve activation of the MAPK pathway. (uea.ac.uk)
  • Immunohistochemistry analysis in human prostate and skeletal muscle tissues using AMAb91461 antibody. (bio-connect.nl)
  • Myasthenia gravis (MG) is a relatively rare acquired, autoimmune disorder caused by an antibody-mediated blockade of neuromuscular transmission resulting in skeletal muscle weakness and rapid muscle fatigue. (medscape.com)
  • [ 1 , 2 ] Although the chief target of the autoimmune attack in most cases is the skeletal muscle nicotinic acetylcholine receptor (nAChR), other antigenic targets that are components of the neuromuscular junction (NMJ) have also been implicated. (medscape.com)
Anti-laminin3
  • The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)
  • Endogenous laminin α5 and bound Lu-Fc were detected with anti-laminin α5 domain LEb/L4b and anti-human IgG, as indicated. (bio-rad-antibodies.com)
  • The anti- laminin degrading activity of a peptide which binds to the receptor, but has no active site, will be tested in specific aim #3. (grantome.com)
Fibronectin and laminin1
  • The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
Proteins6
  • We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
  • We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
  • In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)
  • Abcam: antibodies, proteins, kits. (abcam.com)
  • A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. (frontiersin.org)
  • An Illustration of the Laminin-111 complex depicting the domain organization Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. (pearltrees.com)
Polyclonal antibody1
  • This effect was obtained with a polyclonal antibody as well as with one from 12 monoclonal antibodies raised against the native enzyme purified from chicken gizzard. (morulaivf.com)
Protein7
  • We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
  • Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
  • Immunoblot assays using human epidermal extracts and BP180 NC16a domain recombinant protein confirmed the presence of IgG antibodies against BP180 and recombinant BP180 NC16a domain protein. (morulaivf.com)
  • Synaptic abnormalities and cytoplasmic glutamate receptor aggregates in contactin associated protein-like 2/Caspr2 knockout neurons. (antibodiesinc.com)
  • Stimulated muscarinic acetylcholine receptors (M2Rs) release Gβγ subunits, which slow heart rate by activating a G protein-gated K + channel (GIRK). (elifesciences.org)
  • The higher rate of Gβγ release is attributable to a faster G protein coupled receptor - G protein trimer association rate in M2R compared to β2AR. (elifesciences.org)
  • These two branches control heart rate by stimulating different G protein-coupled receptors (GPCRs), which in turn activate ion channels that modify the electrical properties of cardiac pacemaker cells ( DiFrancesco, 1993 ). (elifesciences.org)
Binds1
  • One of the components of the complex, alpha-dystroglycan binds both laminin of muscle (laminin-2) and agrin of muscle basement membranes. (rupress.org)
Tyrosine4
  • PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. (thermofisher.com)
  • PDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. (thermofisher.com)
  • The HER (erbB) family of transmembrane receptor tyrosine kinases is one of the cytostatic targets in tumor cell growth and survival. (medscape.com)
  • Neurotrophins bind two classes of receptors, the receptor tyrosine kinase family of Trk (A, B, and C) receptors and the p75 neurotrophin receptor (NTR). (jneurosci.org)
Recombinant1
  • Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
Isotype Control1
  • An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
Western blot4
  • Western blot was performed using Anti-ZO-1 Monoclonal Antibody (ZO1-1A12) (Product # 33-9100) and a 225 kDa band corresponding to ZO-1 was observed across along with uncharacterized band. (thermofisher.com)
  • Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band. (antibodiesinc.com)
  • Western blot analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ITGA1 antibody. (bio-connect.nl)
  • The amount of activated RhoA is determined by a western blot using a RhoA specific antibody. (cytoskeleton.com)
Secondary Antibodies1
  • The final sequence was exported as a QuickTime film and still frames from this film sequence had been chosen to generate.Neurite outgrowth assessmentFor neurite outgrowth measurement, cells have been fixed and processed for confocal microscopy working with a mouse monoclonal anti-tubulin antibody plus a rabbit polyclonal G antibody, followed by labeling with rhodamine- and FITC-conjugated secondary antibodies. (scdinhibitor.com)
Immunoglobulins1
  • Antibodies, which are also commonly referred to as immunoglobulins, contain four polypeptides: two longer polypeptides ("heavy chains") that are identical to one another and two shorter polypeptides ("light chains") that are identical to one another. (justia.com)
Membrane4
  • We suggest that the dystroglycan complex acts as a receptor for basement membrane components during epithelial morphogenesis. (rupress.org)
  • Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
  • In five dogs, IgG variably recognized the basement membrane of laminin-332-deficient human skin (three dogs negative, two dogs positive). (morulaivf.com)
  • Normal neuromuscular junction showing a presynaptic terminal with a motor nerve ending in an enlargement (bouton terminale): Synaptic cleft and postsynaptic membrane with multiple folds and embedded with several acetylcholine receptors. (medscape.com)
Transmembrane receptor1
  • Classic signaling involves IL-6 binding to a specific transmembrane receptor termed IL-6Rα. (jneurosci.org)
Bound4
  • When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. (bdbiosciences.com)
  • Also, they bound laminin in a glucocorticoid-dependent manner. (uea.ac.uk)
  • Bound Lu-Fc was detected with an antibody against human IgG as described above. (bio-rad-antibodies.com)
  • The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. (thermofisher.com)
Gene1
  • Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
Inhibitors1
  • Monoclonal Antibody and Synthetic Peptide Inhibitors of Human Tumor Ce" by Kenneth M. Yamada, Dorothy W. Kennedy et al. (howard.edu)
Mouse3
Integrins3
  • Integrins are heterodimeric, transmembrane receptors that function as mechanosensors, adhesion molecules and signal transduction platforms in a multitude of biological processes. (thno.org)
  • Integrins are heterodimeric cell surface receptors found in nearly all metazoan cell types, composed of non-covalently linked α and β subunits. (thno.org)
  • Integrins are not constitutively active but rather exist in multiple activation states wherein ligand binding stability is related to the activation status of the receptor. (thno.org)
Assays2
  • Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
  • In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
Subunits2
  • The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. (assaygenie.com)
  • Stimulated β2 adrenergic receptors (β2ARs) also release Gβγ subunits, but GIRK is not activated. (elifesciences.org)
Affinity chromatography1
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Antigens1
  • Accordingly, there remains a need for antibodies that do not provoke a strong immune response but yet bind strongly to their antigens and methods for identifying such antibodies. (justia.com)
Reagents1
  • These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans. (antibodiesinc.com)
Postsynaptic1
  • The autoimmune attack occurs when autoantibodies form against the nicotinic acetylcholine postsynaptic receptors at the neuromuscular junction of skeletal muscles (see the image below). (medscape.com)
Inhibitory1
Molecule2
Membranes2
  • Merged pictures showed that laminin α5 and Lu-Fc co-localized at basement membranes. (bio-rad-antibodies.com)
  • Prednisone stabilizes lysosomal membranes and suppresses lymphocyte and antibody production. (medscape.com)
Estrogen2
  • Estrone is only a weak agonist of estrogen receptor but it serves as a precursor for biosynthesis of 17β-estradiol, 16α-hydroxyestrone and catechol estrogens. (helsinki.fi)
  • While 16α-hydroxyestrone has relatively weak affinity for estrogen receptor, it has prolonged effect due to covalent binding to the receptor. (helsinki.fi)
Glomeruli1
Agonist1
  • The Effects of NLY01, a Novel Glucagon-Like Peptide-1 Receptor Agonist, on Cuprizone-Induced Demyelination and Remyelination: Challenges and Future Perspectives. (neurotree.org)
Epidermal2
  • Structural features of Caspr2 include epidermal growth factor repeats, laminin G domains, F5/8 type C domain, fibrinogen-like domains, and PDZ binding sites. (antibodiesinc.com)
  • This family, which includes epidermal growth factor receptor (EGFR), plays a pivotal role in normal cell growth, lineage determination, repair, and functional differentiation. (medscape.com)
Extracellular1
  • The results show that the receptors were localised in the cytoplasm in myoblasts, in the nucleus in myotubes and in the extracellular matrix, in satellite cells and in the proximity of mitochondria in adult muscle fibres. (uea.ac.uk)
Human3
Catalog2
  • The following product was used in this experiment: ZO-1 Monoclonal Antibody (ZO1-1A12) from Thermo Fisher Scientific, catalog # 33-9100, RRID AB_2533147. (thermofisher.com)
  • The following product was used in this experiment: CD140b (PDGFRB) Monoclonal Antibody (APB5), PE, eBioscience™ from Thermo Fisher Scientific, catalog # 12-1402-81, RRID AB_529484. (thermofisher.com)
Actin1
  • Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)
Molecular1
  • Analysis of receptor expression is currently hampered by the lack of molecular probes such as antibodies and cDNA's. (grantome.com)
Alpha3
  • A similar increase of expression on day two was previously shown for laminin alpha 1 chain. (rupress.org)
  • Monoclonal antibodies known to block binding of alpha-dystroglycan to laminin-1 perturbed development of epithelium in kidney organ culture, whereas control antibodies did not do so. (rupress.org)
  • It is likely that this involves binding of alpha-dystroglycan to E3 fragment of laminin-1. (rupress.org)
Therapeutic3
  • Researchers have modified antibodies in various ways in order to study their function or to improve their utility as therapeutic agents. (justia.com)
  • The vascular endothelial progress issue (VEGF)/VEGF receptor (VEGFR) axis is an important regulator of angiogenesis and vital therapeutic goal in most cancers. (ncbcs.org)
  • Although the cellular controls of urokinase and its receptor are poorly understood, they represent ideal therapeutic targets and may ultimately yield anti-metastatic agents. (grantome.com)
Tumor1
  • Urokinase may be involved in tumor metastases as evidenced by the ability of anti-urokinase antibodies to block this phenomenon in chicken embryos. (grantome.com)
Tissue1
Basal2
  • In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. (bmj.com)
  • The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, and adhesion. (pearltrees.com)
Stain1
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
Mechanism1
  • The colocalisation with laminin indicates a mechanism of interaction between smooth muscle cells and mast cells that may be important in the role of mast cells in the process of fibrosis. (bmj.com)