Monoclonal antibody antigens cd81. Medical search. Frequent questions

The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells. (exbio.cz)
Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. (thermofisher.com)
Mouse monoclonal to CD81.COB81 reacts with the CD81, a target for anti-proliferative antigen (TAPA-1) with 26 kDa MW, which ia a member of the TM4SF tetraspanin family. (techuniq.com)
The RA3.3A1/6.1 monoclonal antibody reacts with mouse B220 also known as CD45R. (bioxcell.com)

While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.jp)

Truncated soluble forms of E2 have been used as models to study virus interaction with the putative HCV receptor CD81, but they may not fully mimic E2 structures on the virion. (ox.ac.uk)
CD81 has been also identified as a receptor for the hepatitis C virus. (exbio.cz)
CD4 is an accessory protein for major histocompatibility complex (MHC) class-II antigen/T-cell receptor interaction. (ptglab.com)
Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. (neobiotechnologies.com)
CD4 is a co-receptor of the T cell receptor (TCR) and assists the latter in communicating with antigen-presenting cells . (wikidoc.org)

RESULTS: Mice immunization with rNS5B in a mixture with dnC60 at doses of 250 g/mouse significantly induced humoral response: a dose-dependent increase in IgG1 antibody titers was 720 times higher than in the absence of fullerene. (bvsalud.org)
Flow cytometry analysis of CD19 was performed by staining Raji CD19 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82, yellow histogram) or 0.25 µg CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ (Product # 12-0199-42, blue histogram). (thermofisher.com)

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.jp)

CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. (exbio.cz)
Human CD81 is a member of the tetraspan family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion and cellular activation. (adipogen.com)
The TCR-CD3 complex is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. (ptglab.com)
In B-lymphocytes, endocytosis of MHC I and MHC II molecules is important for the cross-priming and presentation of labile antigens, respectively. (silverchair.com)
Glycoproteins are sometimes acknowledged as not-self molecules by antibodies triggering the onset of extreme autoimmune illnesses resembling A number of Sclerosis (MS). Thus, the event of antigen-mimicking biomarkers represents a sexy technique for an early analysis of the illness. (korilog.com)
T cells displaying CD4 molecules (and not CD8 ) on their surface, therefore, are specific for antigens presented by MHC II and not by MHC class I (they are MHC class II-restricted ). (wikidoc.org)
But that's not all - Hycult also has new antibodies to target complement C7 and clusterin, two molecules associated with the formation of the TCC/MAC-complex. (biolynx.ca)

All three glycoprotein forms interacted with human CD81 in an in vitro binding assay, allowing us to test a panel of well-characterized anti-E2 monoclonal antibodies (MAbs) for their ability to inhibit the glycoprotein-CD81 interaction. (ox.ac.uk)
CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). (biolegend.com)
Based on the available data and research through clinical development, a vaccine antigen based on the E1E2 glycoprotein complex appears to be the best choice, but robust induction of humoral and cellular responses leading to virus neutralisation has not yet been achieved. (bvsalud.org)
On this conformation CSF114(Glc) binds the monoclonal antibody mAb8-18C5 equally to the myelin oligodendrocyte glycoprotein MOG, which is a identified MS auto-antigen, thus explaining its diagnostic exercise. (korilog.com)

An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)

CD81 play role as a member of CD19/CD21/Leu-13 signal transdiction complex. (techuniq.com)
CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation. (biolegend.com)
CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. (ptglab.com)

Incubate with primary antibody for 1 hr at room temperature in the dark in staining chambers. (immuquest.com)
If you are looking to compare multiple protein samples on the same gel and want to keep exosomal protein complexes intact, you should check out the fast and gentle Dynabeads-based immunoprecipitation method using Invitrogen Protein A or Protein G in combination with your primary antibody of choice. (thermofisher.com)
The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. (cellsignal.com)
The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. (cellsignal.com)
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. (cellsignal.com)

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. (thermofisher.com)
DX5 is a monoclonal antibody which binds to CD49b. (wikidoc.org)

Protein analysis -Specific monoclonal antibodies ( CD9 , CD63 , and CD81 ) allow for detection of cellular and exosomal antigens by Western blot analysis . (thermofisher.com)
The recent failure of a T-cell vaccine based on the use of viral vectors expressing the HCV non-structural protein sequences to prevent chronic hepatitis C in drug users has pointed out that the induction of neutralizing antibodies (NAbs) will be essential in future vaccine candidates. (bvsalud.org)
As with most antibodies, this antibody interacts with Protein A in S. aureus culture supernatant via the Fc region. (multxpert.com)
This assessment can also be done by measuring antibody responses following active immunization with protein or polysaccharide antigens. (medscape.com)
In children who have completed immunizations with diphtheria, pertussis, and tetanus (DPT) or Hib-conjugated vaccines, the antibody response to protein antigens can be tested in adults and older children by measuring IgG antibodies to tetanus or diphtheria toxoid and H influenzae type b (Hib) polysaccharide antigen. (medscape.com)
Assessment of responses to polysaccharide antigens is important in patients older than 18-24 months because these responses may be deficient in some patients who can respond normally to protein antigens. (medscape.com)
Protein conjugated pneumococcal vaccine elicit antibody responses that are T-cell dependent. (medscape.com)
Therefore, antibody responses should be measured to polysaccharide antigens that are not present in the protein-conjugated pneumococcal vaccine. (medscape.com)
The protein has no known natural ligand [4] and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. (wikidoc.org)
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
CUSABIO is a leading biotechnology company specialized in production of high quality products for gene, protein, antibody, ELISA kit. (cusabio.com)

These peptide usually contains the epitope recognized by the antibody. (affbiotech.com)

At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.jp)

When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. (bdbiosciences.com)
Here, we compared the CD81-binding characteristics of truncated E2 (E2(660)) and full-length (FL) E1E2 complex expressed in mammalian cells, and of HCV virus-like particles (VLPs) generated in insect cells. (ox.ac.uk)
CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. (exbio.cz)
Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. (exbio.cz)
Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. (exbio.cz)
Single-cycle virus production assays in CD81-deficient Huh7-derived cells demonstrated that these changes did not affect replication but increased HCV assembly and specific infectivity as early as 24 h posttransfection. (birmingham.ac.uk)
Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
ERY974 is designed to bring T cells in to close proximity with cancer cells to maximise antibody-activated killing capacity (both antibody induced and T cell directed) against the malignant cells. (guidetopharmacology.org)
Confocal immunofluorescent analysis of Ramos cells, untreated (left) or IgM-treated (middle), and Jurkat cells (right) using Phospho-CD79A (Tyr182) Antibody (green). (cellsignal.com)
To imagine principal cilia cells developing on gelatin-coated cup coverslips for 48 hours in moderate formulated with 0.5% FBS are tagged with antibodies against acetylated tubulin (Sigma T7451) or γ-tubulin (Sigma T5326) and visualized under a Nikon E600 microscope. (techuniq.com)
CD81 is broadly expressed on hemapoietic cells and enothelial and epithelial cells, but absent from erythrocytes and platelets as well as neutrophils. (techuniq.com)
The antibody can be used for detection of alphahemolysin produced secreted from S. aureus cells. (parasites-world.com)
B-lymphocyte antigen CD20 or CD20 is an activated- glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase ( CD45 R+, CD117 +) and progressively increasing in concentration until maturity. (wikidoc.org)
An inflammation response that mobilizes both T and B cells results in the creation of antibodies against these cells, causing them to become less responsive to insulin by an as-yet unknown mechanism and promoting hypertension , hypertriglyceridemia , and arteriosclerosis , hallmarks of the metabolic syndrome . (wikidoc.org)
1 2 Cell surface antigens CD38 and CD138 can be used to distinguish normal cells from clonal plasma cells, but more extensive use of immunophenotyping has been limited by a lack of universally accepted markers of MM. 3-5 Once diagnosed, the current standard-of-care for MM includes immunomodulatory drugs, proteasome inhibitors, steroids, and antibody therapies. (bmj.com)
Here we find that allogeneic tumour rejection is initiated in mice by naturally occurring tumour-binding IgG antibodies, which enable dendritic cells (DCs) to internalize tumour antigens and subsequently activate tumour-reactive T cells. (bioxcell.com)
To assess the clinical relevance of these findings, we studied antibodies and cells from patients with lung cancer. (bioxcell.com)
T cells from these patients responded vigorously to autologous tumour antigens after culture with allogeneic-IgG-loaded DCs, recapitulating our findings in mice. (bioxcell.com)

Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. (exbio.cz)

Since HCV rapidly accumulates mutations, vaccines must elicit the production of broadly neutralising antibodies (bnAbs) in a reproducible fashion. (bvsalud.org)
They are generated by immunizing an animal with an antigen to elicit an immune response. (cellsignal.jp)

Prepare blocking reagent, this is also the antibody diluent. (immuquest.com)
Use the Dynabeads-based CD9 , CD63 , CD81 , or EpCam -specific reagent , or combine the Streptavidin reagent with your choice of a biotinylated antibody to purify any population based on a surface antigen. (thermofisher.com)
You can analyze subsets from total exosomes enriched from cell culture using Invitrogen CD9 , CD63 , CD81 , or EpCam -specific reagent, or use the Invitrogen Streptavidin reagent in combination with your choice of biotinylated antibody. (thermofisher.com)
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD81 (M38) PE antibody (20 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) PE antibody (20 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
The Antibody Detection In Elisa reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (chipready.com)

What is a Recombinant Antibody and Why is it Important? (cellsignal.jp)
Recombinant antibodies offer several key advantages compared to traditional antibodies. (cellsignal.jp)
As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis. (cellsignal.jp)
What is a Recombinant Antibody? (cellsignal.jp)
Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.jp)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.jp)
Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.jp)
Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. (cellsignal.jp)
Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. (cellsignal.jp)
Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.jp)
SDS-PAGE Analysis Purified EGFR Recombinant Mouse Monoclonal Antibody (rGFR/1667). (neobiotechnologies.com)
That's why we've invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality. (stratech.co.uk)

CD20 is the target of the monoclonal antibodies rituximab , ocrelizumab , obinutuzumab , ofatumumab , ibritumomab tiuxetan , tositumomab , and ublituximab , which are all active agents in the treatment of all B cell lymphomas , leukemias , and B cell-mediated autoimmune diseases. (wikidoc.org)

Left Plot) or BD Horizon™ R718 Mouse Anti-Human CD19 antibody (Cat. (bdbiosciences.com)
Human whole blood was stained with the BD Horizon™ BV605 Mouse Anti-Human CD19 antibody (Cat. (bdbiosciences.com)

Gli3 was discovered with two rabbit polyclonal antibodies (Chen et al. (techuniq.com)

Febrile or phlogistic reactions are thought to be secondary to immune aggregates that fix complement, either IgG aggregates or IgG-antigen, complexes. (medscape.com)

In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. (cellsignal.jp)
CD19 deficiency leads to reduced proliferative responses to BCR stimulation in vitro and reduced antibody production following vaccination. (ptglab.com)
CSF114(Glc) is an artificial glucopeptide detecting in vitro MS-specific auto-antibodies, and it's actively utilized in diagnostics and analysis to observe and quantify MS-associated Ig ranges. (korilog.com)

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. (cellsignal.com)

CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6. (biolegend.com)

During this time prepare the secondary antibody. (immuquest.com)
Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody. (cellsignal.com)

A diagnosis of common variable immunodeficiency (CVID) is based on defective functional antibody formation, usually accompanied by decreased (not absent) serum immunoglobulin (Ig)G and IgA levels, generally (not invariably) decreased serum IgM, and exclusion of other known causes of antibody deficiency. (medscape.com)
The common immunologic defect in patients with common variable immunodeficiency is defective antibody formation. (medscape.com)

Generation of monoclonal antibodies to integrin-associated proteins. (wikipedia.org)

Recent work with CD81-null mice indicates CD81 is important for development of humoral immune response. (adipogen.com)

The antibody detects the antigen produced by S. aureus strains in Western Blotting and ELISA and neutralizes the hemolytic activity of the toxin. (multxpert.com)
Phospho-ICAM1 (Tyr512) Antibody detects endogenous levels of ICAM1 only when phosphorylated at Tyrosine 512. (affbiotech.com)

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies, as a companion to validation data generated by CST scientists. (cellsignal.com)
Pneumococcal (unconjugated) or meningococcal vaccines are commercially available polysaccharide antigens. (medscape.com)

This antibody does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. (multxpert.com)
Antibody Elisa Laboratories manufactures the antibody detection in elisa reagents distributed by Genprice. (chipready.com)

Endogenous Sufu was precipitated using a goat polyclonal antibody (M-15 Santa Cruz Biotech) immobilized on proteins A-Agarose beads (Santa Cruz Biotech). (techuniq.com)
Peptides loaded on MHC I are mainly derived from endogenous antigens. (silverchair.com)

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis . (biolegend.com)
Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. (biolegend.com)
The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. (bioxcell.com)

Rat monoclonal antibody recognizing the CD4 mouse T-cell subset marker. (inserm-transfert.com)
This monoclonal antibody is directed against a fixative resistant T-cell associated antigen. (inserm-transfert.com)
Fritzsching B, Schwer B, Kartenbeck J, Pedal A, Horejsi V, Ott M: Release and intercellular transfer of cell surface CD81 via microparticles. (exbio.cz)
Association of CD81 with integrins appears to be functionally important in the regulation of cell adhesion and activation. (adipogen.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. (thermofisher.com)
The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. (thermofisher.com)
ERY974 is an investigational cancer immunotherapy who's mechanism of action puts it in the family of mAbs referred to as T cell redirecting antibodies (or TRABs). (guidetopharmacology.org)
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.jp)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.jp)
Western Blot Analysis of A431 cell lysate using EGFR-Monospecific Mouse Monoclonal Antibody (rGFR/1667). (neobiotechnologies.com)
Obese mice administered anti-B cell CD-20 antibodies, however, did not become less responsive to insulin and as a result did not develop diabetes mellitus or the metabolic syndrome, the posited mechanism being that anti-CD20 antibodies rendered the T cell antibodies dysfunctional and therefore powerless to cause insulin desensitivity by a B cell antibody-modulated autoimmune response. (wikidoc.org)
The RA3.3A1/6.1 antibody is commonly used for in vivo B cell depletion. (bioxcell.com)

However, MAbs specific for regions 432-443, 436-443 and 436-447 inhibited the interaction of VLPs, but not of E2(660) or the FL E1E2 complex with CD81, indicating the existence of structural differences amongst the E2 forms. (ox.ac.uk)

Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. (exbio.cz)

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. (affbiotech.com)

The antibody can be used for detection and neutralization of alpha-toxin produced by virulent strains of S. aureus. (multxpert.com)

MAbs specific for E2 amino acid (aa) regions 396-407, 412-423 and 528-535 blocked binding to CD81 of all antigens tested. (ox.ac.uk)

The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. (bdbiosciences.com)
The antibody was conjugated with BD Horizon™ BV605 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV605 were removed. (bdbiosciences.com)
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. (exbio.cz)

One issue that has arisen in developing an HCV vaccine (and many other vaccines as well) is the platform used for antigen delivery. (bvsalud.org)
This study has implications for investigations requiring high virus concentrations, such as studies of HCV particle composition and development of whole-virus vaccine antigens. (birmingham.ac.uk)
Alternatively antibody response to typhoid-Vi antigen can be measured following typhoid vaccine administration. (medscape.com)

MHC I presents peptides to cytotoxic T-lymphocytes, whereas MHC II displays peptides or larger antigen fragments to T-lymphocytes with helper or regulator function ( Jensen, 2007 ). (silverchair.com)
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. (affbiotech.com)

Interestingly, the E2 hypervariable region 1 (HVR1) mutation T385P caused (i) increased sensitivity to neutralizing patient IgG and human monoclonal antibodies AR3A and AR4A and (ii) increased accessibility of the CD81 binding site without affecting the usage of CD81 and SR-BI. (birmingham.ac.uk)

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. (biolegend.com)
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. (biolegend.com)

Mouse monoclonal antibody (mAb) reactive to full length Staphylococcus aureus alpha-hemolysin (alpha-toxin). (multxpert.com)

Assessment of the ability to produce functional antibodies can be obtained by measuring antibody responses to natural antigens or those antigens to which the population commonly is exposed. (medscape.com)

IgG subclass determination is of limited value in assessing patients with clinical immunodeficiency because functional antibody deficiency may be present despite normal IgG subclass levels. (medscape.com)

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