Monoclonal antibodies integrin beta chains. Medical search. Frequent questions

The CD49b protein is an integrin alpha subunit. (wikipedia.org)
Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. (assaygenie.com)
The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. (assaygenie.com)
This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. (assaygenie.com)
CD18, also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin, is a 90 - 95 kD type I glycoprotein. (biolegend.com)
The basic subunit of beta-crystallins. (lookformedical.com)
The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. (embl.de)
Dependence on beta subunit association and prediction of domains. (embl.de)
The alphaM subunit of integrin Mac-1 contains several distinct regions in its extracellular segment. (embl.de)
We have used monoclonal antibodies (mAbs) as probes to study the dependence of the structure of different regions of the alphaM subunit on association with the beta2 subunit in the alphaM/beta2 heterodimer. (embl.de)
By contrast, four mAbs to the beta-propeller domain did not react with the unassociated alphaM precursor nor with the uncomplexed alphaM subunit expressed on COS cell surface. (embl.de)
These results suggest that folding of different beta-propeller subregions is coordinate and is dependent on association with the beta2 subunit. (embl.de)
This suggests that much of the region C-terminal to the beta-propeller domain folds independently of association with the beta2 subunit. (embl.de)

Staining of M21 cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.5 µg of Anti-Human CD51/CD61 (Integrin alpha/beta 3) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). (thermofisher.com)

CD49b+antigen at the U.S. National Library of Medicine Medical Subject Headings (MeSH) PDBe-KB provides an overview of all the structure information available in the PDB for Human Integrin alpha 2 Moroi M, Jung SM (1997). (wikipedia.org)
Some antibodies require special fixation methods or antigen retrieval steps. (sysy.com)
I mmuno h isto c hemistry (IHC-P) of f ormalin f ixed, p araffin e mbedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the "Remarks" section). (sysy.com)
however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)

Primary Antibodies are guaranteed for 1 year from date of receipt. (novusbio.com)

Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 ( MCA551 ) High power. (bio-rad-antibodies.com)

Integrin alpha-2, or CD49b (cluster of differentiation 49b), is a protein which in humans is encoded by the CD49b gene. (wikipedia.org)
The ITGB3 protein product is the integrin beta chain beta 3. (revoluciondelosgladiolos.org)
CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. (exbio.cz)
This antibody is reactive against recombinant protein in ELISA. (novusbio.com)
Integrin E Rabbit Polyclonal Antibody detects endogenous levels of Integrin E protein. (ebiocell.com)
VAP-1 was originally characterised by a monoclonal antibody (mAb 1B2) that was capable of inhibiting lymphocyte adhesion to high endothelial venules (HEV) and immobilised purified VAP-1 protein [ 6 ] but it subsequently transpired that the enzymatic activity of VAP-1 is also involved in its ability to promote transendothelial migration. (biomedcentral.com)
Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
Immunoblot assays using human epidermal extracts and BP180 NC16a domain recombinant protein confirmed the presence of IgG antibodies against BP180 and recombinant BP180 NC16a domain protein. (morulaivf.com)
CD47 ( C luster of D ifferentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene . (wikidoc.org)
CD47 belongs to the immunoglobulin superfamily [1] and partners with membrane integrins and also binds the ligands thrombospondin-1 ( TSP-1 ) and signal-regulatory protein alpha ( SIRPα ). (wikidoc.org)

DX5 is a monoclonal antibody which binds to CD49b. (wikidoc.org)
The Ha2/5 monoclonal antibody specifically binds to the 130 kDa integrin β1 chain (CD29). (bdbiosciences.com)

Monoclonal antibody 9EG7 defines a novel beta 1 integrin epitope induced by soluble ligand and manganese, but inhibited by calcium. (xenbase.org)
The signaling inhibitory antibody quickly induces ligand-independent receptor clustering and internalization via each caveolin and clathrin-mediated pathways. (kashbiotech.com)
Fc-ARMs are composed of an Fc-binding peptide and a concentrating on ligand , enabling the exploitation of endogenous antibodies via fixed affinity to the Fc area of antibodies, whose sequence is conserved in distinction to the Fab area. (kashbiotech.com)
Integrins are not constitutively active but rather exist in multiple activation states wherein ligand binding stability is related to the activation status of the receptor. (thno.org)
Extracellular factors that influence integrin activation are ligand binding, divalent cation concentration, chemokine signaling and mechanical stress. (thno.org)
Activity Assay: A representative lot of the original monoclonal antibody has been shown to convert alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding. (ximbio.com)

CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. (thermofisher.com)
The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. (assaygenie.com)
Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. (assaygenie.com)
Integrins are heterodimeric cell surface receptors found in nearly all metazoan cell types, composed of non-covalently linked α and β subunits. (thno.org)
Beta-crystallins exist as oligomers formed from acidic (BETA-CRYSTALLIN A CHAIN) and basic (BETA-CRYSTALLIN B CHAIN) subunits. (lookformedical.com)
The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits. (lookformedical.com)
They contain both alpha and beta subunits. (embl.de)
The four mAbs were mapped to three subregions in three different beta-sheets, making it unlikely that each recognized an interface between the alpha and beta subunits. (embl.de)
Our data provide new insights into how different domains in the integrin alpha and beta subunits may interact. (embl.de)

Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. (thermofisher.com)
The Ha2/5 hamster anti-rat CD29 monoclonal antibody cross-reacts with mouse thymocytes, splenocytes, and peripheral lymph node leukocytes. (bdbiosciences.com)
Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. (thermofisher.com)
The antibody reacts with human platelets and megakaryocytes. (yoproteins.eu)

Integrin alpha 2b, (ITGA2b) also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. (rndsystems.com)
8. Khaspekova SG, Vyzova TV, Lukin VV, Tikhomirov OIu, Berndt M, Kouns W, Mazurov AV. (1996) [Conformational changes of the platelet membrane glycoprotein IIb-IIIa complex stimulated by a monoclonal antibody to the N-terminal segment of glycoprotein IIIa]. (yoproteins.eu)

The DX5 monoclonal antibody recognizes mouse CD49b. (wikipedia.org)

This effect was obtained with a polyclonal antibody as well as with one from 12 monoclonal antibodies raised against the native enzyme purified from chicken gizzard. (morulaivf.com)

Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. (wikipedia.org)
Elevated expression of laminins (LN) and integrins in the synovial membrane of rheumatoid arthritis (RA) versus osteoarthritis patients has been reported but metabolic effects of attachment of synovial fibroblasts (SF) to LN are not well studied. (biomedcentral.com)
Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
CD47 interacts with several membrane integrins, most commonly integrin avb3. (wikidoc.org)

The mouse monoclonal antibody PAC-1 recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation. (exbio.cz)

Intracellular signaling events that converge on the cytoplasmic domain of integrin tails are also capable of activating the high affinity state. (thno.org)

The following product was used in this experiment: CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), Biotin, eBioscience™ from Thermo Fisher Scientific, catalog # 13-0519-82, RRID AB_529536. (thermofisher.com)
CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. (thermofisher.com)
With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αV (CD51), it forms αVβ1 integrin. (bdbiosciences.com)

Integrin alpha II combines with Integrin beta 1 to form the alpha2/beta1 integrin duplex. (ximbio.com)

Similarly, depletion of either Rasip1 or Arhgap29 in cultured ECs blocks in vitro lumen formation, fundamentally alters the cytoskeleton, and reduces integrin-dependent adhesion to ECM . (xenbase.org)
The Ha2/5 antibody blocks in vitro adhesion of CD29-expressing cells to collagen. (bdbiosciences.com)
We present that Fc-ARM concentrating on folate receptor-α (FR-α) redirects a clinically used antibody combination to FR-α + most cancers cells, leading to most cancers cell lysis by pure killer cells in vitro . (kashbiotech.com)
Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)

E�H) E9.5 littermate mouse embryos showing defects in Rasip1 / embryos (F) and yolk sacs (H). (I�L) Whole-mount Flk1 -lacZ beta-galactosidase staining of Rasip1+/ (I and K) and Rasip1 / (J and L) yolk sacs (I and J) and embryonic heads (K and L), showing narrow Rasip1 / blood vessels that fail to remodel. (xenbase.org)
Mouse monoclonal antibody to Integrin beta 3. (revoluciondelosgladiolos.org)
Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes. (thermofisher.com)
Detects mouse Integrin alpha 2b/CD41 in direct ELISAs. (rndsystems.com)
Moreover, Fc-ARMs recruited antibodies to suppress tumor progress in a mouse mannequin. (kashbiotech.com)
LGALS3 Mouse Recombinant produced in E. coli is a single polypeptide chain containing 287 amino acids (1-264) and having a molecular mass of 29.8 kDa. (prospecbio.com)

Direct inhibition of EGFR activation is considered the primary mechanism for antitumor activity for cetuximab, but other mechanisms including antibody-dependent cellular cytotoxicity (ADCC) and receptor internalization are likely to play an important role as well (see investigated 30 patients treated with cetuximab, 11 of whom had a Celiprolol HCl response, for mutations in KRAS, BRAF and PIK3CA by direct sequencing as well as EGFR copy number by chromogenic hybridization. (revoluciondelosgladiolos.org)
The non-inhibitory antibody additionally effectively endocytoses through clathrin with out inducing receptor clustering however with slower lysosomal co-localization kinetics. (kashbiotech.com)
Integrin alpha-4/beta-7 is also a receptor for MADCAM1. (abcam.co.jp)

Whole cells corresponding to human Integrin alpha5. (ximbio.com)

Integrins are heterodimeric, transmembrane receptors that function as mechanosensors, adhesion molecules and signal transduction platforms in a multitude of biological processes. (thno.org)

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Trikha M, Timar J, Lundy SK, Szekeres K, Cai Y, Porter AT, Honn KV: The high affinity alphaIIb beta3 integrin is involved in invasion of human melanoma cells. (exbio.cz)
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. (biolegend.com)
The antibody was purified by affinity chromatography. (angioproteomie.com)

Expression of the α1, β1 and γ1 chains of LN1 laminin by SF was investigated by quantitative RT-PCR and immunocytochemistry. (biomedcentral.com)
In SF, mRNAs encoding α1, β1 and γ1 laminin - which encode the proteins for LN1 - were detected by quantitative RT-PCR and transcript amounts encoding the α1 and β1 chains of LN1 were higher than mRNA encoding the LN1 γ1 chain. (biomedcentral.com)
However, using monoclonal antibodies to laminin α1 or β1 proteins, staining signals were very weak. (biomedcentral.com)
Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
In all nine dogs, IgG auto-antibodies detected purified human laminin-332 by immunoblotting. (morulaivf.com)

ST3GAL5 is known to play a part in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. (prospecbio.com)

The product of this gene belongs to the integrin alpha chain family. (assaygenie.com)

Integrins are integral cell-surfaceproteins composed of an alpha chain and a beta chain. (revoluciondelosgladiolos.org)
Integrin beta 3 is found along with the alpha IIb chain inplatelets. (revoluciondelosgladiolos.org)
The antibody is specific to the integrin complex alpha 2b beta 3, but not to the dissociated integrins. (yoproteins.eu)
Alpha, beta, and delta crystallins occur in avian and reptilian lenses, while alpha, beta, and gamma crystallins occur in all other lenses. (lookformedical.com)
CD49e is the integrin alpha 5 chain. (ximbio.com)
This product was previously labelled as Integrin alpha 4. (abcam.co.jp)
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. (abcam.co.jp)
Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. (abcam.co.jp)
Galectin-3 mediates with the alpha-3, beta-1 integrin the stimulation by cspg4 of endothelial cells migration. (prospecbio.com)
Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. (transcriptionfactor.org)
ST3 Beta-Galactoside Alpha-2,3-Sialyltransferase 5, also know as ST3GAL5 belongs to the glycosyltransferase family 29 and localized to the Golgi apparatus. (prospecbio.com)
Integrin alpha (beta-propellor repeats). (embl.de)
Alpha integrins are proposed to contain a domain containing a 7-fold repeat that adopts a beta-propellor fold. (embl.de)

Nonetheless, present molecular designs solely allow the usage of a small fraction of endogenous antibodies, limiting the therapeutic potential. (kashbiotech.com)
Cancer-specific cell surface antigens are ideal therapeutic targets for monoclonal antibody (mAb)-based therapy. (bvsalud.org)

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. (bdbiosciences.com)

2001) [Inhibition of thrombocyte aggregation by F(ab')2-fragments of monoclonal antibodies FraMon (CRC64) to glycoproteins IIb-IIIa]. (yoproteins.eu)
Therefore, pharmacological inhibition of integrins is of great interest for the treatment and prevention of disease. (thno.org)
In the absence of integrin ligation, these processes are abrogated therefore pharmacological inhibition of integrin ligation is of great interest for the therapy of numerous diseases resulting from abberant integrin mediated signaling. (thno.org)

Integrin-mediated interactions with the extracellular matrix (ECM) are required for the attachment, cytoskeletal organization, mechanosensing, migration, proliferation, differentiation and survival of cells in the context of a multitude of biological processes including fertilization, implantation and embryonic development, immune response, bone resorption and platelet aggregation. (thno.org)
Each domain consists of two antiparallel beta sheets and is folded topologically identically to single fibronectin type III domains from the extracellular matrix proteins tenascin and fibronectin. (embl.de)
Integrins are cell adhesion molecules that mediate cell-extracellular matrix and cell-cell interactions. (embl.de)

Immunoisolation or pulldown of a target molecule using an antibody. (sysy.com)
An unusually small rotation between domains 9 and 10 creates a distinctive binding site, in which the RGD loop from domain 10 and the "synergy" region from domain 9 are on the same face of FN7-10 and thus easily accessible to a single integrin molecule. (embl.de)

These interactions involve several families of adhesion molecules including integrins and immunoglobulin superfamily members and selectins and carbohydrate-bearing ligands. (biomedcentral.com)
These interactions result in CD47/integrin complexes that affect a range of cell functions including adhesion, spreading and migration. (wikidoc.org)
The RGD sequences is involved in interactions with integrin. (embl.de)

Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 0.5mg/ml. (angioproteomie.com)

The order of amino acids as they occur in a polypeptide chain. (lookformedical.com)
ST3GAL5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 359 amino acids (83-418a.a) and having a molecular mass of 41kDa. (prospecbio.com)

The antibody does not react with platelets from patients with the hereditary deficiency of GP IIb-IIIa (Glanzmann's thrombasthenia). (yoproteins.eu)

The following product was used in this experiment: CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscience™ from Thermo Fisher Scientific, catalog # 25-0114-82, RRID AB_469590. (thermofisher.com)

Redirecting endogenous antibodies in the bloodstream to tumor cells utilizing artificial molecules is a promising strategy to set off anti-tumor immune responses. (kashbiotech.com)

The antibody does not cross-react to rabbit or dog. (yoproteins.eu)

CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. (bdbiosciences.com)

CD18 and associated α chains function in the adhesion and signaling in hematopoietic cells. (biolegend.com)

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
Integrins are involved in cell adhesion and also participate in cell-surface-mediated signalling. (wikipedia.org)
Comparison of integrin expression and terminal differentiation capacity in cell lines derived from oral squamous cell carcinomas. (ximbio.com)
Integrins are known to participate in cell adhesion as well as cell-surface mediatedsignalling. (revoluciondelosgladiolos.org)
Interestingly, the most primordial defense responses employed by myeloid cells against pathogens, such as complement activation, antibody-dependent cell cytotoxicity and phagocytosis, actually seem to favor cancer progression. (frontiersin.org)
Integrin mediated cellular adhesion is dynamic process that is both indicative of and influenced by cell status. (thno.org)
Integrin ligation via cell-to-cell and cell-to-matrix contacts transduces intracellular signaling events in a process called 'outside-in' signaling (Fig. 2 ). (thno.org)
Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. (lookformedical.com)
In addition to adhesion, integrins are known to participate in cell surface mediated signalling. (ximbio.com)
Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. (ximbio.com)
On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. (abcam.co.jp)
VAP-1 mediates adhesion via heavily sialidated side chains but it also functions as a cell surface expressed ectoenzyme-a primary amine oxidase. (biomedcentral.com)

Integrins also function in pathological processes such as inflammation, wound healing, angiogenesis, and tumor metastasis. (thno.org)
Here, we have described a cleavage product of the COL6α3 chain, endotrophin (ETP), which serves as the major mediator of the COL6-mediated tumor effects. (jci.org)

Right here, we develop NOTCH3-targeted antibody drug conjugates (NOTCH3-ADCs) by bioconjugation of an auristatin microtubule inhibitor via a protease cleavable linker to 2 antibodies with differential skills to inhibit signaling. (kashbiotech.com)

Applications Reported: This 23C6 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
Applications Tested: This 23C6 antibody has been tested by flow cytometric analysis of peripheral blood cells. (thermofisher.com)
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis . (biolegend.com)

Herein, this review examines and evaluates the various drugs and compounds targeting integrins and the disease states in which they are implicated. (thno.org)

Anatomy28

Organisms12

Diseases4

Chemicals and Drugs129

Analytical, Diagnostic and Therapeutic Techniques and Equipment40

Phenomena and Processes47

Disciplines and Occupations2

Information Science3

Health Care2

Subunit13

Isotype1

Antigen4

Primary antibodies1

Clone1

Protein10

Binds2

Ligand6

Subunits9

Reacts4

Glycoprotein2

CD49b1

Polyclonal Antibody1

Membrane4

Known as integrin1

Domain of integrin1

CD513

Beta1 integrin1

Vitro6

Mouse6

Receptor3

Human Integrin1

Transmembrane1

Affinity4

Laminin7

Signal transduction1

Gene1

Alpha13

Therapeutic2

Purification1

Inhibition3

Extracellular3

Molecule2

Interactions3

Concentration1

Polypeptide2

IIIa1

EBioscience1

Endogenous1

Rabbit1

CD291

CD181

Cell12

Tumor2

Conjugates1

Flow cytometric analysis3

Compounds1