• The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)
  • The peripheral blood mononuclear cells (PBMCs) have a round nucleus ( Delves, 2016 ). (frontiersin.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found 16 significant regulated pathways in imDCs and 10 significant regulated pathways in mDCs, including the phagosome, cell adhesion signaling pathway, and inflammatory mediator regulation of TRP channels pathway. (frontiersin.org)
  • A pharmacological inhibitor of TLR3 considerably reduced cytokine and chemokine production by CD4 + and CD8 + T cells but not by CD14 + monocytes, highlighting divergent signaling pathways of immune cells in response to COVID-19 plasma exosomes. (wphobby.com)
  • Dendritic cells (DCs), which are the most important antigen-presenting cells (APCs) that send signals to the T cells, mainly participate in the pathogenesis of many diseases with immunoregulatory mechanisms, such as AR. (frontiersin.org)
  • These exosomes can interact with immune cells, such as T cells, macrophages, and dendritic cells, to modulate proinflammatory immune responses. (wphobby.com)
  • 1- 5 Moreover, we have previously shown that the Th1 cell response in fetal gut explants results in villous atrophy and crypt cell hyperplasia, 6, 7 thus supporting the role of local Th1 cell inflammation in promoting CD associated tissue damage. (bmj.com)
  • In coeliac disease (CD) mucosa, the histological lesion is associated with marked infiltration of T helper cell type 1 (Th1) cells. (bmj.com)
  • T-bet and STAT-4, two Th1 promoting transcription factors, and STAT-6 and GATA-3, transcription factors which govern T helper cell type 2 (Th2) cell polarisation, were examined in duodenal biopsies by western blotting. (bmj.com)
  • There is now little doubt that in coeliac disease (CD) the histological lesion is associated with a predominant T helper cell type 1 (Th1) cell response. (bmj.com)
  • Several studies have clearly demonstrated that distinct cytokine activated signalling and transcription factors regulate the commitment of a naïve T cell along the Th1 or T helper cell type 2 (Th2) phenotype, as well as maintenance of the polarised phenotype. (bmj.com)
  • Intestinal macrophages were later co-cultured with intestinal epithelial cells to observe the degree of epithelial cell injury. (impactaging.com)
  • Therefore, the M1 macrophages play an important pro-inflammatory role in IBD, which represent the major cell type inducing IBD progression [ 4 ]. (impactaging.com)
  • It is currently discovered that, Th1 cells are a kind of pro-inflammatory T cells, which exert mutual regulation with natural killer (NK) cells and macrophages [ 5 ]. (impactaging.com)
  • In research on IBD, the change in Th cell proportions and the interaction with macrophages have not been completely illustrated yet. (impactaging.com)
  • Th1 cells are the inflammatory cells with high expression of IFN-γ and TNF-α [ 7 ], therefore, this work analyzed the proportion of Th cells in tissues of IBD patients, so as to further reveal its regulatory relation with macrophages. (impactaging.com)
  • Nuclear dust is often apparent, and a variable number of eosinophils and mononuclear cells are present within the dermal infiltrate. (medscape.com)
  • Furthermore, evidence for an important pathogenetic role for effector B cell-derived profibrotic IL-6 and TNF-α, as well as for protective effects of anti-inflammatory IL-10, has been published recently [ 6 ]. (biomedcentral.com)
  • Pretreatment with CLL antagonized the effect of Th1 cells, reduced the intestinal tissue inflammatory response and mucosal barrier permeability. (impactaging.com)
  • Th2 cells are a kind of anti-inflammatory cells, which exert their effect via IL-10 [ 6 ]. (impactaging.com)
  • Here, we report that plasma exosomes of COVID-19 patients contain SARS-CoV-2 double stranded RNA (dsRNA) and stimulate robust production of interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α), and other inflammatory cytokines and chemokines by human peripheral mononuclear cells. (wphobby.com)
  • Here, we investigated the therapeutic potential of ibrutinib, a Bruton's tyrosine kinase (BTK) inhibitor used in B cell malignancies, to alter B cell pathology in SSc in an in vitro model of autoimmunity. (biomedcentral.com)
  • The Bruton's tyrosine kinase (BTK) inhibitor ibrutinib could inhibit hyperactivated B cell subpopulations to counteract underlying pathogenetic mechanisms. (biomedcentral.com)
  • Besides, cells were treated with the STAT3 inhibitor to further detect the macrophage polarization level. (impactaging.com)
  • Complete B cell depletion with rituximab showed better efficacy with regard to the reduction of skin fibrosis and respiratory restriction in a case-control study [ 8 ]. (biomedcentral.com)
  • The clinical improvement in both trials might have been limited by the lack of specificity, as depletion of all B cell subsets eliminates the protective effects conveyed by Bregs in the context of autoimmunity. (biomedcentral.com)
  • Nuclear magnetic resonance (NMR). (lu.se)
  • A sign of immediate international recognition of the new Physical Chemistry team is that the Division has already been selected as a partner, with responsibilities in light scattering and nuclear magnetic resonance, in the EU-sponsored infrastructure in soft matter (see p. 29 for details). (lu.se)
  • In a flow cytometry analysis of phosphorylated NFκB, an important transcription factor in the induction of innate immune responses in B cells, significantly less activation was observed with ibrutinib treatment. (biomedcentral.com)
  • COVID-19 plasma exosomes induced proinflammatory responses in CD4 + T cells, CD8 + T cells, and CD14 + monocytes but not significantly in regulatory T cells, Th17 T cells, or central memory T cells. (wphobby.com)
  • These exosomes significantly increase expression of endosomal toll-like receptor 3 (TLR3), TLR7, TLR8, and TLR9 in peripheral T cells and monocytes. (wphobby.com)
  • PBMCs and sorted B cells of 24 patients with SSc were used for functional testing after stimulation with hypomethylated DNA fragments (CpG) to induce an innate immune response. (biomedcentral.com)
  • Ibrutinib was able to reduce the production of the profibrotic hallmark cytokines IL-6 and TNF-α mainly from the effector B cell population in patients with SSc. (biomedcentral.com)
  • Importantly, small doses of ibrutinib (0.1 μM) preserved the production of immunoregulatory IL-10 while effectively inhibiting hyperactivated, profibrotic effector B cells. (biomedcentral.com)
  • The sensitive equilibrium between effector B cells and regulatory B cells (Bregs) is disrupted, and immunoregulatory Bregs prove to be numerically and functionally impaired [ 5 ]. (biomedcentral.com)
  • This work aimed to investigate the role of helper T cell 1 (Th1) in chronic colitis and its immunoregulatory mechanism. (impactaging.com)
  • thereafter, Th1 cells were induced in vitro and amplified before they were injected intraperitoneally. (impactaging.com)
  • Histologically, bullous systemic lupus erythematosus is characterized by dermoepidermal separation beneath an intact epidermis. (medscape.com)
  • T-LGLL can present as failure of hematopoiesis or immune-mediated destruction of one or several cell lines including RBCs, neutrophils, and platelets. (oncotarget.com)
  • Alterations to the B cell compartment maintain the hyperreactivity and chronic activation of large portions of the immune system. (biomedcentral.com)
  • Virus-infected cells release virus-associated exosomes, extracellular vesicles of endocytic origin, into the blood to deliver viral cargoes able to regulate immune responses. (wphobby.com)
  • Many important questions remain however regarding factors which induce and maintain Th1 cell polarisation in CD. (bmj.com)
  • However, the molecular mechanisms which regulate Th1 cell differentiation in CD mucosa are unknown. (bmj.com)
  • Circulating Tumor Cells (CTCs) are cells that break free from the tumor and circulate in the blood of cancer patients, forming metastases. (utwente.nl)
  • The CANCER-ID project aims to validate technologies for Circulating Tumor Cells (CTCs), circulating free DNA (cfDNA) and circulating cell free microRNAs (cfmiRs) as blood-based biomarkers to determine the absence/presence of drug targets and assess response to treatment. (utwente.nl)
  • The two cancer types that will be investigated are Non-Small Cell Lung Cancer and Her2-therapy resistant metastatic breast cancer. (utwente.nl)
  • Pure red cell aplasia (PRCA) was present in 18 subjects (50%) of our subjects and responded well to methotrexate. (oncotarget.com)
  • To analyse expression of transcription factors which control the Th1 cell commitment in CD. (bmj.com)
  • T-cell large granular lymphocytic leukemia (T-LGLL) is a rare haematologic neoplasm. (oncotarget.com)
  • T-cell large granular lymphocytic leukemia (T-LGLL) is a lympho-proliferative neoplasm of cytotoxic T cells [ 1 ]. (oncotarget.com)
  • Consequently, different approaches have been tested to address the hyperactivation of B cells in SSc. (biomedcentral.com)
  • The specific inhibition of autoreactive, profibrotic, and chronically activated B cell subsets represents a more promising approach to achieve effective treatment of SSc. (biomedcentral.com)
  • Accumulating evidence suggests that B cells are involved in SSc beyond the mere production of autoantibodies. (biomedcentral.com)
  • T-LGLL associated pure red cell aplasia (PRCA) accounts for a significant portion of secondary PRCA [ 3 ]. (oncotarget.com)