• CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. (biolegend.com)
  • As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils. (biolegend.com)
  • This adhesion allows the T cell receptor (TCR) to interact with major histocompatibility complex (MHC) molecules on the surface of the dendritic cell, which, upon binding between the TCR, the MHC, and the peptide coupled to the MHC, facilitates T cell activation. (wikipedia.org)
  • This process is believed to involve binding between ICAM3 and CD14 receptors, which are a type of cell surface receptor expressed on macrophages and other phagocytes. (wikipedia.org)
  • CD14, a receptor expressed on the surface of phagocytes, can also bind ICAM3. (wikipedia.org)
  • We found that receptors in the Toll-like receptor pathway, such as Tlr2 and CD14, were increased under LPS and HSV stimulation. (frontiersin.org)
  • There was strong up-regulation of TLR2, CD14, MYD88, IKKA and NFKB as the dominant toll-like receptor signaling pathway. (scienceblog.com)
  • The CD14 antibody binds specifically to the 53-55 kilodalton (kDa) glycosylphosphatidylinositol (GPI)-anchored single-chain glycoprotein, CD14, also known as the LPS receptor or LPS-R. (bdbiosciences.com)
  • CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. (thermofisher.com)
  • it begins as a singular molecule in its biosynthesis and ends as a singular molecule in the mammalian receptor. (europeanpharmaceuticalreview.com)
  • LPS initially binds to the LBP (LPS-binding protein) and transfer it via the CD14-enhanced mechanism to a receptor complex including TLR-4 and MD-2. (vin.com)
  • The CD14 molecule has been reported to be a receptor for Endotoxin. (biometec.de)
  • The CD14 antibody, clone MφP9, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood monocytes from a patient with rheumatoid arthritis. (bdbiosciences.com)
  • The following product was used in this experiment: CD14 Monoclonal Antibody (61D3), Super Bright™ 645, eBioscience™ from Thermo Fisher Scientific, catalog # 64-0149-42, RRID AB_2662458. (thermofisher.com)
  • Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. (thermofisher.com)
  • Human PBMC stimulated with 250 ng/ml LPS overnight were stained with either (A) Mouse Anti-Human IL-1 beta /IL-1F2 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC201T) or (B) Mouse IgG1Isotype Control ( IC002T ) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody ( FAB3832A ). (rndsystems.com)
  • CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. (biolegend.com)
  • P-selectin enhances generation of CD14+CD16+ dentritic-like cells and inhibits macrophage maturation from human peripheral blood monocytes. (bdbiosciences.com)
  • CD14++ monocytes, CD14+/CD16+ subset and soluble CD14 as biological markers of inflammatory systemic diseases and monitoring immunosuppressive therapy. (bdbiosciences.com)
  • Monocytes were isolated from PBMCs using the RosetteSep Human Monocytes Enrichment Kit followed by CD14-positive selection by MACS. (nih.gov)
  • CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. (thermofisher.com)
  • Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14. (thermofisher.com)
  • CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. (thermofisher.com)
  • Results: The presence of MMF during the differentiation of monocytes into iDCs resulted in cells that retained low levels of CD14 and hardly expressed CD1a. (eur.nl)
  • As disease onset neared, microglia expressed increased CCL2 and other chemotaxis-associated molecules, which led to the recruitment of monocytes to the CNS by spinal cord-derived microglia. (nih.gov)
  • In humans with ALS, the analogous monocytes (CD14+CD16-) exhibited an ALS-specific microRNA inflammatory signature similar to that observed in the ALS mouse model, linking the animal model and the human disease. (nih.gov)
  • The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. (biometec.de)
  • Jayaram Y, Hogg N. Surface expression of CD14 molecules on human neutrophils. (bdbiosciences.com)
  • Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. (cytekbio.com)
  • Inhibition of calcitriol-induced monocyte CD14 expression by uremic toxins: Role of purines. (ugent.be)
  • Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 160Gd-anti-CD14 (M5E2). (biolegend.com)
  • 4 Once isolated, the LPS molecule is transferred to MD-2, which sits in TLR4, and brings about a transmembrane signalling event. (europeanpharmaceuticalreview.com)
  • TLR4 not only takes the measure of the molecule but also signals a response based upon the underlying variance in sub-molecular moieties. (europeanpharmaceuticalreview.com)
  • Synthetic molecules derived from natural sugars with a positively charged amino group or ammonium salt and two lipophilic chains have been shown to inhibit TLR4 activation in vitro and in vivo. (unimib.it)
  • To characterize the mechanism of action of this class of molecules, we investigated possible interactions with the extracellular components that bind and shuttle endotoxin [lipopolysaccharide (LPS)] to TLR4, namely, LBP, CD14, and MD-2. (unimib.it)
  • Dose-dependent inhibition of the transfer of [3H]LOS between the two forms of CD14 was observed with each of three different synthetic compounds that inhibited TLR4 activation but not by another structurally related analogue that lacked TLR4 antagonistic activity. (unimib.it)
  • Saturation transfer difference (STD) NMR data showed direct binding to CD14 by the synthetic TLR4 antagonist mediated principally through the lipid chains of the synthetic compound. (unimib.it)
  • TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. (cytekbio.com)
  • CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. (thermofisher.com)
  • A single molecule of LPS is selected (purified away) from an aggregate by lipopolysaccharide binding protein (LBP)or potentially as a monomer where serum albumin has been found to serve in lieu of LBP. (europeanpharmaceuticalreview.com)
  • Adhesion molecules and cytokines were quantified by ELISA. (unipi.it)
  • The mechanisms involved in this crosstalk are based on cell-to-cell contact and on paracrine secretion of several molecules including growth factors, cytokines and inflammatory mediators [3]. (unicyte.ch)
  • They can be identified using flow cytometry or immunohistochemical staining by their specific expression of proteins such as CD14, CD40, CD11b, CD64, F4/80 (mice)/EMR1 (human), lysozyme M, MAC-1/MAC-3 and CD68. (studybuff.com)
  • Both beta-defensins and their signaling molecules, Toll-Like Receptors, have been identified in the heart of various species. (vin.com)
  • Furthermore CD14 is present in a soluble form in mouse serum, urine and other body fluids. (biometec.de)
  • Goyert SM, Ferrero E. Biochemical analysis of myeloid antigens and cDNA expression of gp55 (CD14). (bdbiosciences.com)
  • To establish whether these cells were differentiated into iDCs, we analysed the expression of cell surface molecules on these cells and the capacity to capture antigens using flow cytometry. (eur.nl)
  • both groups of subjects had similar expression of these molecules. (studybuff.com)
  • Vitamin E-bonded cellulose membrane and hemodialysis bioincompatibility: Absence of an acute benefit on expression of leukocyte surface molecules. (ugent.be)
  • Citrate anticoagulation does not correct cuprophane bioincompatibility as evaluated by the expression of leukocyte surface molecules. (ugent.be)
  • This recognition results in different intracellular pathways being activated, involving the adaptor molecules AP-1 and MyD88 and the transcription factor nuclear factor kappa B (NF- κ B). The NF- κ B transcription factor is a central mediator of the innate immune response, regulating infectious as well as non-infectious stress responses. (vin.com)
  • 2-fold including epithelial adhesion molecules and keratins. (scienceblog.com)
  • It is a unique molecule used as a marker of prokaryotic invasion by metazoan immune systems and occurs as a singular constituent among multiple gram-negative bacteria OM constituents, including phospholipids and surface proteins. (europeanpharmaceuticalreview.com)
  • CSCs were shown to secrete large amount of extracellular vesicles (EVs) that can transfer several molecules (proteins, lipids and nucleic acids) and induce epigenetic changes in target cells. (unicyte.ch)
  • Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a protein that in humans is encoded by the ICAM3 gene. (wikipedia.org)
  • ICAM3 is a 110-160 kDa protein that belongs to the intercellular adhesion molecule (ICAM) family. (wikipedia.org)
  • These peptides are ancient and universal molecules of innate immunity with functions extending far beyond simple antibiotics, including anti-tumor and mitogenic activity, as well as immunomodulation and signal transduction characteristics. (vin.com)
  • Interactions between CD14 and altered ICAM3 molecules on apoptotic cells are believed to help promote phagocytosis of apoptotic cells. (wikipedia.org)
  • Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714-82) (blue histogram) or Anti-Human CD14 Super Bright 645 (purple histogram). (thermofisher.com)
  • Upon maturation, these MMF-iDCs upregulated CD83 and costimulatory molecules and HLA-DR on their surface, indicating that these cells respond to LPS, albeit less than control iDCs. (eur.nl)
  • CD14 is also observed on granulocytes and activated or transformed B-cells. (biometec.de)
  • No inhibition of LPS binding to CD14, binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry). (biometec.de)
  • cells are responsive to the treatment of FLT3 ligand and GM-CSF, the number of LPMs, SPMs, and MHCII+CD11c+CD115+CD14+CD206+ cells are only influenced from the injection of GM-CSF. (bibf1120.com)
  • In addition, the analysis of gene manifestation profiles among MHCII+ peritoneal myeloid mononuclear Balamapimod (MKI-833) cells discloses that MHCII+CD11c+CD115+CD14+CD206+ cells share high similarity with SPMs, whereas MHCII+CD11c+CD115+CD14?CD206? (bibf1120.com)
  • Collectively, our study identifies 2 unique subpopulations of MHCII+CD11c+CD115+ cells, 1) MHCII+CD11c+CD115+CD14?CD206? (bibf1120.com)
  • These cells, surrounding the hair follicles, secrete signaling molecules that control the progression of the hair cycle. (jcadonline.com)
  • molecules secreted by the tumor and tumor-related stromal cells, promoting their recruitment from bone marrow into the circulation and subsequent engraftment within tumor microenvironment [14]. (unicyte.ch)
  • It has been found that some of the SVF cell types possess regenerative and anti-inflammatory potentials in damaged tissues due to their ability to secrete growth factors and anti-inflammatory molecules. (jcadonline.com)
  • Common mechanisms of recognition, engulfment, and degradation of the different types of cargo are assumed, but very little is known about the shared and specific molecules involved in the phagocytosis of each target by microglia. (frontiersin.org)
  • Staining was performed using our Staining Intracellular Molecules protocol. (rndsystems.com)
  • These compounds also inhibited transfer of the [3H]LOS monomer from full-length CD14 to a truncated, polyhistidine-tagged CD14. (unimib.it)
  • The amphipathicity of these molecules determines their folding pattern and is believed to govern the antimicrobial effect. (vin.com)