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  • gene expression
  • Embodiments of the present invention provide methods for targeted inactivation of viral genomes using engineered zinc-finger proteins containing nuclease domains (ZFN), and targeted inactivation of viral gene expression using RNA interference. (patentgenius.com)
  • The last decades have witnessed the explosion of scientific interest around gene expression control mechanisms at the RNA level. (jove.com)
  • assay
  • Although several state-of-art strategies are currently available for dosing or visualizing such fleeing molecules, Northern Blot assay remains the eligible approach in molecular biology for immediate and accurate evaluation of RNA expression. (jove.com)
  • transduction
  • The TCR and several coreceptors thus become localized at the T cell surface, leading to the formation of signal complexes with intracellular molecules and the initiation of a transduction cascade, resulting in the execution of effector functions. (rupress.org)
  • Viral
  • The endo-siRNA pathway, which produces small double-stranded RNAs in Drosophila, appears to be nonessential for robust development of the embryo under ambient uniform temperature and to be necessary only for viral defense. (jove.com)
  • species
  • Such a revolutionary perspective has been accompanied and triggered by the development of powerful technologies for profiling short RNAs expression, both at the high-throughput level (genome-wide identification) or as single-candidate analysis (steady state accumulation of specific species). (jove.com)
  • cell
  • Streptavidin is devised as a handle to assemble biotinylated cell penetrating peptides (e.g., transactivating transcriptional activator (TAT)), as well as an insulator between the positive charge of TAT and the dense negative charge of RNA. (ucsb.edu)
  • However, we hypothesized that small RNAs may regulate the embryos response to temperature, as a ribonucleoprotein complex has been previously shown to mediate mammalian cell response to heat shock. (jove.com)
  • molecular
  • This branch of molecular biology has been greatly fueled by the discovery of noncoding RNAs as major players in post-transcriptional regulation. (jove.com)
  • major
  • Amino acid starvation also provoked stabilization of the RNAs coding for major virulence regulators, such as SaeRS and SarA, independently of RSH. (asm.org)
  • amount
  • A very limited amount of RNA is required and the use of material from flow cytometry-isolated cells can be also envisaged. (jove.com)
  • produce
  • We hypothesized that cCAF stimulates fibroblasts to produce these matrix molecules. (biomedcentral.com)
  • These previously unknown functions for chemokines suggest that cCAF, the chicken orthologue of human IL-8, enhances healing by rapidly chemoattracting fibroblasts into the wound site and stimulating them to produce ECM molecules, leading to precocious development of granulation tissue. (biomedcentral.com)
  • physical
  • Linear or (in bacteria and organelles) circular DNA molecule that constitutes the basic physical block of heredity. (bmj.com)
  • uses
  • KCCM 10093 pigments-some of which have demonstrated newly-identified functionality-and the relevant uses of these molecules for pharmaceutical or nutraceutical purposes. (mdpi.com)
  • specific
  • Indeed, we found that although tumor-specific CD103 + TIL-derived cytotoxic T lymphocyte (CTL) clones are able to kill E-cadherin + /intercellular adhesion molecule 1 − autologous tumor cells, CD103 − peripheral blood lymphocyte (PBL)-derived counterparts are inefficient. (rupress.org)
  • cells
  • p-SMAC, which is formed upon ligation of LFA-1 on CTLs by high densities of intercellular adhesion molecule (ICAM)-1 on target cells, is essential for directing released cytolytic granules to the surface of tumor cells near c-SMAC and effective lysis of the latter cells by CTLs ( 4 - 6 ). (rupress.org)