AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
DextransDextran SulfateCell Adhesion MoleculesIron-Dextran ComplexColitisMolecular Sequence DataAmino Acid SequenceSmall Molecule LibrariesProtein BindingFluorescein-5-isothiocyanateCell LineKineticsModels, MolecularCells, CulturedDextranaseBinding SitesMice, Inbred C57BLMolecular WeightBase SequenceSignal TransductionDEAE-DextranNeural Cell Adhesion MoleculesProtein ConformationAntibodies, MonoclonalColonCell AdhesionIntercellular Adhesion Molecule-1Mice, Inbred BALB CFluorescent DyesAntigens, CDRecombinant ProteinsStructure-Activity RelationshipPeptidesLigandsMembrane ProteinsT-LymphocytesTime FactorsMolecular StructureDNAWaterCell MembraneModels, BiologicalProtein Structure, TertiaryMicroscopy, FluorescenceRNA, MessengerDisease Models, AnimalCrystallography, X-RayMembrane GlycoproteinsPeptide FragmentsEscherichia coliIntestinal MucosaMutationMice, KnockoutHistocompatibility Antigens Class IIElectrophoresis, Polyacrylamide GelMicroscopy, ElectronMolecular ConformationPermeabilityHistocompatibility Antigens Class IFlow CytometryProteinsEpitopesMacromolecular SubstancesLymphocyte ActivationCattleHydrogen-Ion ConcentrationVascular Cell Adhesion Molecule-1TransfectionRabbitsNucleic Acid ConformationSolubilityCytokinesCapillary PermeabilityRecombinant Fusion ProteinsLeuconostocFluoresceinsNeural Cell Adhesion Molecule L1HeparinPolymersCloning, MolecularGene Expression RegulationBacterial ProteinsImmunohistochemistryPolysaccharidesMicroscopy, ConfocalCarrier ProteinsAntibodiesBlotting, WesternThermodynamicsCricetinaeSequence Homology, Amino AcidCell Line, TumorAntigens, SurfaceTemperaturePlasma SubstitutesBiological TransportSpectrometry, FluorescenceMacrophagesInflammationAntigen Presentation
FITC-DextranSulfate sodiumSodiumFluorescentFluorescein isothiocyanatePerfusionPolymerPolysaccharideAdhesion moleculesVascularMolecularMiceConcentrationsPolymersNanoparticlesBindHeparinProteinsBiotinPermeabilityInflammationVivoEpithelialFluidGlucoseNeutralEfficiencyTumorMembraneDiseasesSmallProteinTypicallyIronMethodsStudyExpressionPropertiesCellApplicationProcessNatureSugarLargeMade
FITC-Dextran16
  • FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. (sigmaaldrich.com)
  • FITC-dextran has been used to study plant cell wall porosity and capillary permeability. (sigmaaldrich.com)
  • Plasma proteins have been shown not to bind to FITC-dextran. (sigmaaldrich.com)
  • FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. (sigmaaldrich.com)
  • FITC-Dextran 250 (FD 250) may be used as a model compound for development of methods to deliver molecules into protoplasts and to study drug release from structures such as hydrogels and liposomes. (sigmaaldrich.com)
  • In vivo, FITC-dextran is stable for more than 24 hours. (sigmaaldrich.com)
  • Additional information about FITC-labelled polysaccarides including FITC-Dextran, FITC-DEAE-dextran (FDD), FITC-carboxymethyl-dextran (FCM-Dextran). (sigmaaldrich.com)
  • In 2.5D culture, treatment with HMGB1 induced permeability of FITC-dextran into the lumen formed by human lung, nasal and intestinal epithelial cells. (anobase.org)
  • The intention of the current examine was to guage retro-orbital (RO) injection of fluorescein-labeled isothiocyanate dextran (FITC-dextran) as a technique of demonstrating retinal neovascularization (NV) and avascular areas in oxygen-induced retinopathy (OIR) mice. (teitell-lab.com)
  • Totally different concentrations of FITC-dextran have been used to match the efficacy of this agent in perfusing the retinal vessels. (teitell-lab.com)
  • The vitreous blood vessels and extent of NV have been assessed in P17 OIR mice utilizing FITC-dextran and in contrast with the corresponding measurements obtained following isolectin B4 staining or the mix of each strategies. (teitell-lab.com)
  • The fluorescence of small vessels and neovascular tufts may very well be noticed clearly following RO injection of 0.05 ml of 25 mg/ml or 50 mg/ml FITC-dextran. (teitell-lab.com)
  • Vitreous blood stream was steadily decreased from P0 to P5 and finally disappeared in P17 OIR mice, as demonstrated by FITC-dextran perfusion. (teitell-lab.com)
  • The retinal NV areas assessed by isolectin B4 have been bigger than these assessed by FITC-dextran, however the retinal avascular areas have been smaller. (teitell-lab.com)
  • RO injection of FITC-dextran mixed with isolectin B4 is an efficient, optimum methodology for assessing the NV space and avascular space. (teitell-lab.com)
  • For validating the experimental apparatus and the protocol for data analysis, the diffusion D of FITC-Dextran molecules of 4, 40 and 250 kDa was first quantified. (bvsalud.org)
Sulfate sodium3
  • The purpose of this study was to investigate the protective effect of GLP-2 on Dextran sulfate sodium (DSS) -induced UC in mice and its potential mechanism. (researchsquare.com)
  • In the present study we examined the protective potential of exogenously administered human umbilical cord derived mesenchymal stem cells (UCMSCs) against Dextran Sulfate Sodium (DSS) induced acute colitis in immunodeficient NOD.CB 17 - Prkdc scid /J mice with particular attention to endoplasmic reticulum (ER) stress. (biomedcentral.com)
  • Dextran sulfate sodium is the sodium salt of dextran sulfate. (unipex.com)
Sodium1
Fluorescent4
  • The method consisted of a single intravenous injection of 12 mg of a large 150-kDa carboxy-methyl dextran conjugated to a fluorescent rhodamine-derived dye as the PV marker (PVM), and 35 mg of a small 5-kDa carboxy-methyl dextran conjugated to fluorescein, the renal clearance marker. (karger.com)
  • Bristles made of amino acids stick out from the iron ball with fluorescent "reporter" molecules attached to their tips. (eurekalert.org)
  • Through fluorescent microscopy, the dynamics of molecules and nanoparticles was estimated within the gel, under different operating conditions. (bvsalud.org)
  • mesenteric arteries (MA) and veins (MV) were mounted on glass cannulas, intravascularly filled with fluorescent dextran and incrementally pressurized above their in vivo physiological values. (bvsalud.org)
Fluorescein isothiocyanate2
Perfusion3
  • Perfusion Fluid CNS Dextran MW 500 kDa 3%, 5 ml, pkg. (harvardapparatus.com)
  • We recommend the use of the CMA Microdialysis CNS Perfusion fluid with Dextran 500 kDa 3% in order to stabilize the pressure within the probe and limit ultra-filtration. (harvardapparatus.com)
  • The CNS Perfusion Fluid with Dextran 500 kDa 3% is suitable for our CNS high cut-off probes with 55 and 100 kDa cut-off for rats and mice. (harvardapparatus.com)
Polymer7
  • Dextran is a polymer of anhydroglucose. (sigmaaldrich.com)
  • Biotin-Dextran-Fluorescein is a dually labeled dextran polymer. (creativepegworks.com)
  • There is at least one biotin and one fluorescein molecule per polymer. (creativepegworks.com)
  • G-Sep™ Size Exclusion Columns are composed of high performance rigid resin made up of co-polymer of agarose and dextran. (gbiosciences.com)
  • Degree of substitution: 1 mol % substitution and at least one dye molecule per dextran polymer. (creativepegworks.com)
  • In one embodiment, the nanocage is covalently attached to a polymer shell (e.g. a dextran shell). (justia.com)
  • [ 1 , 2 ] PEG, a water-soluble linear polymer, appears to accelerate antibody-RBC binding by steric exclusion of water molecules in the diluents and to promote antibody detection. (medscape.com)
Polysaccharide1
  • Dextran, a neutral and hydrophilic polysaccharide, was functionalized with maleimide or vinyl sulfone groups which are able to form covalent bonds with nucleophilic thiols via a thiol-Michael addition reaction. (universiteitleiden.nl)
Adhesion molecules7
  • Briefly, IgSFs are adhesion molecules that bind to themselves (homophilic) or compatible IgSFs (heterophilic) across cell-cell junctions. (elifesciences.org)
  • As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils. (biolegend.com)
  • Adhesion molecules are important for the extravasation of neutrophils into organs where they may exhibit detrimental effects. (biomedcentral.com)
  • Therefore, we investigated the in vitro effect of DHEA on the expression pattern of adhesion molecules of human endothelial cells and neutrophils. (biomedcentral.com)
  • Activated by cytokines and chemokines, leukocytes and endothelial cells express distinct adhesion molecules on their cell surfaces [ 9 ]. (biomedcentral.com)
  • These adhesion molecules enable the deceleration of blood cells on the endothelial layer in order to enable subsequent diapedesis. (biomedcentral.com)
  • The importance of adhesion molecules in traumatic and septic diseases has been widely recognized. (biomedcentral.com)
Vascular2
Molecular8
  • Refined over time by scientists such as Robert Boyle, Amedeo Avogadro, Jean Perrin, and Linus Pauling, the study of molecules is today known as molecular physics or molecular chemistry. (wikipedia.org)
  • Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. (sigmaaldrich.com)
  • Disclosed is a covalently-linked multilayered three-dimensional matrix comprising capture molecules, linkers and spacers (referred to as a Molecular Net) for specific and sensitive analyte capture from a sample. (justia.com)
  • Also disclosed herein is a Molecular Net comprising covalently-linked multilayered three-dimensional matrix comprising more than one type of capture molecule and more than one type of linker and may comprise one or more spacer for specific and sensitive capture of more than one type of analyte from a sample. (justia.com)
  • Use of various capture molecules, linkers and spacers in a Molecular Net may confer unique binding properties to a Molecular Net. (justia.com)
  • Dextran sulfate prehybridization buffer (approximately 20 ml). 10 ml formamide (deionized and molecular biology grade). (iassist2012.org)
  • Traditional molecules for intravenous administration may cause rare but fatal anaphylactic reactions (especially for high molecular weight iron dextran), severe hypotension due to 'labile' iron (iron sucrose or iron gluconate) or need to be given at repeated small doses with a long infusion time in order to avoid toxicity (iron gluconate and iron sucrose). (delveinsight.com)
  • As expected, D reduces with the molecular weight of the dextran molecules. (bvsalud.org)
Mice1
  • Using stereomicroscopy and microscopic examination of plastic sections, dextran dyes dispersed in SNOT are demonstrated in the neuroepithelium of the nose and olfactory bulb of B6;129P2-Omp(tm3Mom)/MomJ mice after intranasal instillation in SNOT. (cdc.gov)
Concentrations1
Polymers2
Nanoparticles1
  • Here, a 'Tissue Chamber' chip is designed and fabricated to study the extravascular transport of small molecules and nanoparticles. (bvsalud.org)
Bind1
Heparin1
  • Although the mechanisms of action of these reagents remain to be clarified, several lines of evidence connect most of the anti-prion agents with molecules of the heparin family. (europa.eu)
Proteins2
  • CMA offers a set of microdialysis probes for the recovery of large molecules (such as proteins, cytokines, growth factors, antibody and neuropeptides…) on awake rodents. (harvardapparatus.com)
  • Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes. (gbiosciences.com)
Biotin5
  • Dextran is labeled with both biotin and fluorescein (FITC), Ex/Em wavelength 494/518 nm. (creativepegworks.com)
  • Description This product is labeled by biotin molecule. (thermalspraydirectory.com)
  • Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). (thermalspraydirectory.com)
  • Biotin binds to streptavidin and avidin with an extremely high affinity, fast on-rate, and high specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of interest. (thermalspraydirectory.com)
  • I would like someone to contact me with more information regarding Biotin-labeled Dextran Sulfate, 5 kDa. (thermalspraydirectory.com)
Permeability1
  • The tricellular tight junction molecule angulin-1/LSR is responsible for the epithelial permeability barrier at tricellular contacts and contributes to various human airway and intestinal inflammatory diseases. (anobase.org)
Inflammation1
Vivo1
  • artérias mesentéricas (MA) e veias (MV) foram montadas em cânulas de vidro, por via intravenosa preenchida com dextran fluorescentes e incremental pressurizado acima de seus valores fisiológicos in vivo. (bvsalud.org)
Epithelial1
  • Published studies on diacetyl and 2,3-pentanedione document their ability to cause airway epithelial necrosis, damage biological molecules, and perturb protein homeostasis. (cdc.gov)
Fluid2
  • The use of probes with large pore size membranes required for the microdialysis of large molecules creates some methodological challenges such as protein aggregation, protein adhesion, ultrafiltration and low fluid recovery/extraction efficiency. (harvardapparatus.com)
  • Endothelial cells regulate the passage of gases, fluid and various molecules across blood vessels by acting as selective filters. (bvsalud.org)
Glucose1
  • However, when glucose enters the picture, it competes with dextran, displacing the dextran molecules and binding to the conA. (technovelgy.com)
Neutral1
  • The glomerular permselectivity to polydisperse neutral dextrans was compared in 6 patients with thin membrane nephropathy (TMN) and 10 healthy controls. (jci.org)
Efficiency1
  • The obtained drug delivery platforms showed high drug loading efficiency and demonstrated sustained release of drug molecules. (universiteitleiden.nl)
Tumor1
  • Conventional drug administration methods such as intravenous injection and oral administration are the main methods for delivering chemotherapeutic molecules to tumor cells. (plos.org)
Membrane2
  • A major barrier for the efficacy of many clinically relevant anti-cancer drugs (e.g. gemcitabine, 5-flurouracil, cisplatin) is the passage of these molecules across biological membranes, whether in the context of transit across an endothelial monolayer or across the plasma membrane of the cancer cell itself [ 5 ]. (plos.org)
  • Clathrin and cargo molecules are assembled into clathrin-coated pits on the plasma membrane together with an adaptor complex called AP-2 that links clathrin with transmembrane receptors, concluding in the formation of mature clathrin-coated vesicles (CCVs). (wormbook.org)
Diseases1
  • Blood Cells, Molecules and Diseases. (aau.dk)
Small4
  • According to Merriam-Webster and the Online Etymology Dictionary, the word "molecule" derives from the Latin "moles" or small unit of mass. (wikipedia.org)
  • Small FITC-dextrans are also used to measure processes such as endocytosis. (sigmaaldrich.com)
  • That releases the reporter molecules, which are so small that they easily make it through the kidney's filtration and go into the urine. (eurekalert.org)
  • The MSD-derived diffusion coefficients were in good agreement with values derived via fluorescence recovery after photobleaching (FRAP), an alternative technique that solely applies to small molecules. (bvsalud.org)
Protein2
  • The use of the microdialysis technique for the recovery of large molecules is of increasing interest as it offers the opportunity to identify patterns of protein expression in a variety of tissue spaces as well as to evaluate useful biomarkers of disease. (harvardapparatus.com)
  • More recently, the realization that macro- principal experimental challenge is to resolve and quantify molecules occupy 20-30% of the intracellular volume has populations of protein oligomers in dynamic equilibrium. (lu.se)
Typically1
  • Atoms and complexes connected by non-covalent interactions, such as hydrogen bonds or ionic bonds, are typically not considered single molecules. (wikipedia.org)
Iron1
  • Consequences of parenteral iron-dextran loading investigated in minipigs. (aau.dk)
Methods1
  • The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity. (sigmaaldrich.com)
Study2
  • CMA offers new complete solutions for the study of large molecules using the microdialysis techniques. (harvardapparatus.com)
  • In the present study, we were able to demonstrate effects of DHEA on the expression of every adhesion molecule investigated. (biomedcentral.com)
Expression2
  • Moreover, Al-positive samples exhibited a significant decrease in the number of apoptotic cells, as well as the expression of the anti-apoptotic molecule BCL-2. (bvsalud.org)
  • Thus, we conclude that one mechanism by which DHEA may exert its protection in animal models is via the differential regulation of adhesion molecule expression. (biomedcentral.com)
Properties2
  • Earlier definitions were less precise, defining molecules as the smallest particles of pure chemical substances that still retain their composition and chemical properties. (wikipedia.org)
  • Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. (sigmaaldrich.com)
Cell1
  • The haemoglobinopathies are a relatively common, heterogeneous group of inherited conditions that are the result of either a quantitative abnormality (e.g. thalassaemia) or structural [e.g. sickle cell anaemia (SCA)] of the globin part of the haemoglobin molecule. (medscape.com)
Application1
  • This thesis describes the design, characterization and application of dextran based crosslinked network. (universiteitleiden.nl)
Process2
  • Key molecules in this recognition process are thought to be members of the immunoglobulin superfamily (IgSFs). (elifesciences.org)
  • New method to form giant unilamellar vesicles was established by assisting the hydration process of a lipid film with a dextran-poly(ethylene glycol) hydrogel film. (universiteitleiden.nl)
Nature1
  • Concepts similar to molecules have been discussed since ancient times, but modern investigation into the nature of molecules and their bonds began in the 17th century. (wikipedia.org)
Sugar1
  • It is double-coated with dextran, a sugar, and polyethylene glycol, a common ingredient in laxatives, to keep the body from disposing of it too quickly. (eurekalert.org)
Large2
  • This definition often breaks down since many substances in ordinary experience, such as rocks, salts, and metals, are composed of large crystalline networks of chemically bonded atoms or ions, but are not made of discrete molecules. (wikipedia.org)
  • Why large molecules in microdialysis research? (harvardapparatus.com)
Made1
  • His 1811 paper "Essay on Determining the Relative Masses of the Elementary Molecules of Bodies", he essentially states, i.e. according to Partington's A Short History of Chemistry, that: The smallest particles of gases are not necessarily simple atoms, but are made up of a certain number of these atoms united by attraction to form a single molecule. (wikipedia.org)