• The PFY-147-EPS was found to be a levan containing a backbone of 2,6-substituted ß-fructoses by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectroscopy. (bvsalud.org)
  • The lab is equipped with state-of-the-art laboratory instruments, such as GC-MS (Gas Chromatography coupled with Mass Spectrometry) and LC-MS (Liquid Chromatography coupled with Mass Spectrometry) and uses common analytical techniques. (laimburg.it)
  • The wide variety of equipment, columns, eluent and operational parameters involved makes high performance liquid chromatography (HPLC) method development seem complex. (pharmtech.com)
  • It is being undertaken using powerful analytical tools, like two-dimensional gel electrophoresis (2-DE) and ultrasensitive mass spectrometry (MS), coupled with high-throughput functional screening assays. (ashwinnaik.com)
  • Mass spectrometry has become a widely used method in protein analysis since the invention of matrix-assisted laser-desorption ionization/time-of-flight (MALDI-TOF) and electrospray ionization (ESI) methods. (ashwinnaik.com)
  • In proteome analysis electrophoretically separated proteins can be identified by mass spectrometry with two different approaches. (ashwinnaik.com)
  • PMF is usually carried out with MALDI-TOF, and sequence tags by nano-ESI tandem mass spectrometry (MS/MS). The sensitivity of protein identification by MS is in the femtomole range. (ashwinnaik.com)
  • SVEVs isolated from lyophilized venoms collected from four different species of snakes ( Agkistrodon contortrix contortrix , Crotalus atrox , Crotalus viridis and Crotalus cerberus oreganus ) were analyzed by mass spectrometry-based proteomic, which allowed the identification of proteins belonging to eight main functional protein classes such as SVMPs, serine proteinases, PLA 2 , LAAO, 5′nucleotidase, C-type lectin, CRISP and Disintegrin. (nature.com)
  • Final determination of target analytes is performed by isotope dilution gas chromatography high-resolution mass spectrometry GC/IDHRMS. (cdc.gov)
  • Serum concentrations are determined using gas chromatography isotope dilution high resolution mass spectrometry (GC/IDHRMS), which minimizes or eliminates many interferences associated with low-resolution measurement of organohalogen compounds. (cdc.gov)
  • Traditionally, polyanhydrides have been prepared by melt condensation polymerization, which results in high molecular weight polymers. (wikipedia.org)
  • Although PEG polymers may undergo enzymatic degradation, significant fluid uptake and swelling dominate the degradation of our formulated PEG:dextran hydrogels, resulting in hydrolytically sensitive materials. (nature.com)
  • Multiple Linear Regression Modeling To Predict the Stability of Polymer-Drug Solid Dispersions: Comparison of the Effects of Polymers and Manufacturing Methods on Solid Dispersion Stability MOLECULAR PHARMACEUTICS. (nottingham.ac.uk)
  • In the second approach peptides after in-gel digestion are fragmented in the mass spectrometer, yielding partial amino acid sequences from the peptides (sequence tags). (ashwinnaik.com)
  • The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method. (bvsalud.org)
  • Myotubes protein synthesis decreased and protein degradation increased together with an enhanced in the chymotrypsin-like enzyme activity, a measure of functional proteasome activity, after treatment with WF. (biomedcentral.com)
  • Atrophy of skeletal muscle was due to a depression in protein synthesis (by 50%) and an increase in protein degradation (by 50%) [ 5 ]. (biomedcentral.com)
  • PIF induces protein degradation in skeletal muscle by induction of the ubiquitin-proteasome proteolytic pathway, which is considered to be the major mechanism by which myotubular proteins are degraded in skeletal muscle [ 12 ]. (biomedcentral.com)
  • Recent results [ 13 ] show that PIF inhibits protein synthesis and induces protein degradation in skeletal muscle through a single step: the activation of the ds RNA-dependent protein kinase (PKR) [ 13 ]. (biomedcentral.com)
  • Protein degradation in this bacterium appears to be triggered by the accumulation of polyP. (go.jp)
  • Here I propose to call the polyP-Lon complex the "stringent protease," and I discuss new insights of protein degradation control in bacteria. (go.jp)
  • This slow degradation rate makes aromatic polyanhydrides less suitable for drug delivery when used as homopolymers, but they can be copolymerized with the aliphatic class to achieve the desired degradation rate. (wikipedia.org)
  • It reflects the relative molecular mass or aromatic condensation degree of humic acid. (researchgate.net)
  • The selected fractions (green line) were loaded onto an SDS-PAGE gel and stained with InstantBlue. (elifesciences.org)
  • The fractions were partially sequenced by Edman's degradation. (biomedcentral.com)
  • Fermentation of β-glucan fractions from barley [average molecular mass (MM), of 243, 172, and 137 kDa] and oats (average MM of 230 and 150 kDa) by the human faecal microbiota was investigated. (megazyme.com)
  • Low molecular mass heparins are salts of sulphated glucosaminoglycans having a mass average molecular mass less than 8000. (chromatographyonline.com)
  • Proteins are separated in 2-DE according to their pI and molecular weight. (ashwinnaik.com)
  • Proteins constitute almost 95% of snake venom's dry weight and are produced and released by venom glands in a solubilized form during a snake bite. (nature.com)
  • In addition, the molecular function of some of the described proteins suggests a central role for SVEVs in the cytotoxicity of the snake venom and sheds new light in the envenomation process. (nature.com)
  • Certain very abundant ribosomal proteins can be the sacrificial substrates targeted for degradation at the onset of the downshift. (go.jp)
  • Alpha-crystallins also act as molecular chaperones that bind to denatured proteins, keep them in solution and thereby maintain the translucency of the lens. (lookformedical.com)
  • After removal of a cancerous brain tumor, the wafer is inserted into the brain releasing a chemotherapy agent at a controlled rate proportional to the degradation rate of the polymer. (wikipedia.org)
  • This quest led to the discovery of PVDF, a semi-crystalline polymer with a well-defined molecular structure. (jiuanchemical.com)
  • low-molecular-weight (LMW) compounds consist of two or three rings, and high-molecular-weight (HMW) compounds, more than three rings [ 3 ]. (omicsonline.org)
  • 15 m length, 0.1 μm film thickness, 0.25 mm ID) enabling the determination of high molecular weight compounds such as decabromodiphenyl ether (BDE-209) having a molecular weight close to 1000 amu. (cdc.gov)
  • NMR Spectroscopy provides a fast and extremely powerful method for "fingerprint'' identification of compounds in complex matrices as well as a tool for determining structure and dynamics of low-, intermediate-molecular weight biomolecules. (laimburg.it)
  • Size exclusion chromatography would normally be considered for analysing high molecular weight compounds (.2000). (pharmtech.com)
  • Characterization of polyanhydrides determines the structure, composition, molecular weight, and thermal properties of the molecule. (wikipedia.org)
  • There are several options for the mass analyzer, the most common combinations being time-of-flight (TOF) connected to MALDI and triple quadrupole, quadrupole-TOF, or ion trap mass analyzer coupled to ESI. (ashwinnaik.com)
  • During this procedure serum lipids are degraded in the sulfuric acid layer while cholesterol is removed in the top layer consisting of activated silica gel. (cdc.gov)
  • We harnessed non-invasive fluorescence imaging to sequentially follow in vivo material-mass loss to model the degradation of materials hydrolytically (PEG:dextran hydrogel) and enzymatically (collagen). (nature.com)
  • However, controlled material development is limited, as degradation in vivo is more complex than in vitro , and in vitro assays are rarely adequate measures of implant behaviour. (nature.com)
  • Loss of material integrity, structure, and eventually mass, progress dependently over time but are dominated by different environmental forces in vitro and in vivo . (nature.com)
  • Moreover, the levan exhibited higher thermal stability with a degradation temperature of 265.63 °C in DSC analysis. (bvsalud.org)
  • The mechanochemical methods can increase the molecular condensation degree and molecular relative mass of humic acid, but reduce the molecular condensation degree and molecular relative mass of fulvic acid. (researchgate.net)
  • I was also co-founder and Technical Development Director of a successful spin-out, Molecular Profiles Ltd (which became Juniper Pharma in 2013 and was acquired by Catalent in 2018). (nottingham.ac.uk)
  • Each PTH amino acid has a characteristic retention time in the RP-chromatography, enabling the identification of the residue by comparing to a standard. (ashwinnaik.com)
  • Animal's and microorganisms' secretions, as well as plant extracts, have been used as folk medicine since the dawn of the humanity [ 1 ].Therefore, molecules found in poisons and venoms are interesting, once they were selected by evolution to act in their molecular targets with high specificity [ 1 , 2 ]. (biomedcentral.com)
  • LCPS intake reduced at significant level high fat diet (HFD)-induced body weight gain, liver weight, total cholesterol and triglyceride in serum and hepatic tissue, respectively. (bvsalud.org)
  • Concentrations of target analytes are reported on two different bases, i.e., (i) fresh weight basis (i.e., pg/g serum) and (ii) lipid weight basis (i.e., ng/g lipid). (cdc.gov)
  • As a consequence of their properties, these biopolymers derived from natural products have found a place of choice in areas as diverse as effluent treatment, papermaking, chemical, food, cosmetic, pharmaceutical, petroleum and textile industries, as well as in analytical chemistry (biosensors) and molecular biology. (benthambooks.com)
  • For this September issue of The Application Notebook should be reaching our audience of analytical chemists, lab managers, and other industry professionals just as the busiest time of the year ramps up in the world of chromatography. (chromatographyonline.com)
  • Catalysts may be used to achieve higher molecular weights and shorter reaction times. (wikipedia.org)
  • PVDF's resistance to chemical degradation contributes to the efficiency and safety of chemical processes. (jiuanchemical.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • Thermal Gravimetric Analysis highlighted degradation temperature of 218 °C, Differential Scanning Calorimetry (DSC) glass transition at 81.47 °C, and Dynamic Mechanical Analysis three frequency-dependent transition peaks. (bvsalud.org)
  • Then they are separated according to their mass-to-charge ratio in the mass analyzer and the separate ions are detected. (ashwinnaik.com)
  • In this study, we evaluated the anti-inflammatory effect of Z. jujuba leaf in LPS-induced RAW264.7 cells and identified the regulatory mechanisms of NF-κB (nuclear factor kappa B) and MAPK (mitogen activated protein kinase) as molecular targets. (kspbtjpb.org)
  • The potential spectrum of bioterrorism ranges from hoaxes and actual use of agents by individuals or groups against others, to state-sponsored terrorism that employs biological warfare (BW) agents and delivery systems that can produce mass casualties. (medscape.com)
  • Edman N-terminal amino acid sequencing is usually done by Edman degradation. (ashwinnaik.com)
  • Molecular docking and molecular dynamics simulations indicated that the peptides should bind well to ACE and interact with amino acid residues and the zinc ion at the ACE active site. (bioinfor.com)
  • They produce hemotoxic venom which causes bleeding, tissue degradation and necrosis. (mdpi.com)
  • Patients with advanced cancer suffer from cachexia, which is characterised by a marked weight loss, and is invariably associated with the presence of tumoral and humoral factors which are mainly responsible for the depletion of fat stores and muscular tissue. (biomedcentral.com)
  • Mass spectrometers consist of the ion source, mass analyzer, ion detector, and data acquisition unit. (ashwinnaik.com)
  • It also has so-called high-resolution mass spectrometers to identify new, unknown substances as well as a Near Infrared device suitable for destruction-free analysis. (laimburg.it)
  • Owing to their safe degradation products, polyanhydrides are considered to be biocompatible. (wikipedia.org)
  • Degradation products of Mer2 full-length protein are also visible. (elifesciences.org)
  • PBDEs are not chemically bound to the flame-retarded material, so they can enter the environment from volatilization, leaching, or degradation of PBDE-containing products. (cdc.gov)
  • Most of the important molecular processes in the cell, such as DNA replication, are performed by large molecular network of protein-protein interactions. (researchsquare.com)
  • In this approach the protein spot of interest is in-gel digested with a specific enzyme, the resulting peptides are extracted from the gel and the molecular weights of these peptides are measured. (ashwinnaik.com)
  • The angiotensin-I-converting enzyme (ACE) plays a critical role in controlling blood pressure, converting the hormone angiotensin I to the potent vasoconstrictor angiotensin II as well as catalysing the degradation of the vasodilator bradykinin, both actions raising blood pressure. (bioinfor.com)
  • These findings confirm the existence of a fine-tuned molecular assembly line where tasks are performed sequentially and without intervening delays. (biomedcentral.com)
  • The two most abundant ions in the isotopic cluster (fragment or molecular ion) are monitored for the target analyte as well as for the 13C-labeled internal-surrogate standard. (cdc.gov)
  • Furthermore, we performed caseinolytic activity test to assess the presence of serine and metalloproteases in skin secretion and it was fractionated by fast liquid protein chromatography using a reverse-phase column. (biomedcentral.com)
  • Morphological structure and physicochemical properties were investigated to assess levan microstructure, degradation temperature and thermomechanical features. (bvsalud.org)
  • PVDF possesses a unique molecular structure and is widely used in various industries due to its versatility and reliability. (jiuanchemical.com)