• Dr. Pryhuber trained as a post-doctoral Proctor Research Scholar and participant in the University of Cincinnati Program of Excellence in Molecular Biology of the Heart and Lung. (rochester.edu)
  • BOOTH, F. W. Application of molecular biology in exercise physiology. (unicamp.br)
  • Molecular and Cellular Biology , 21 (19), 6461-9. (ed.ac.uk)
  • Leveraging our proprietary Spectra TM Network Biology platform and artificial intelligence, we commercialize blood tests revealing a persons' unique molecular disease signature and match such signature to the most effective therapy, ensuring optimal treatment from day one. (b3cnewswire.com)
  • In recent times, significant advancements have been made in elucidating the molecular alterations of the disease, and the results have been an improved understanding of CRC biology, as well as the discovery of biomarkers of diagnostic, prognostic, and therapeutic significance. (org.sa)
  • One recent study conducted in Japan demonstrated that the relative risk of narcolepsy in affected first-degree family members is 10- to 40-fold higher than in the general population, suggesting that genetic factors play an important role in this disease 12 . (nature.com)
  • Genetic polymorphisms that amplify the inflammatory response to infection have been found among patients with schizophrenia, suggesting that genetic factors may confer heightened sensitivity to infection and other prenatal insults. (health.am)
  • RA affects ~1% of the population worldwide ( 2 ), and its pathogenesis may be linked to genetic and environmental factors ( 3 , 4 ). (frontiersin.org)
  • 7 , 8 The aim of this review is to explore the molecular pathology research that has been carried out on CRC from Saudi patients, with a view to understanding the prevalence and clinicopathological correlates of clinically and biologically relevant genetic mutations in Saudi CRC patients. (org.sa)
  • Genetic basis of severe factor XIII deficiency in a large cohort of Indian patients: Identification of fourteen novel mutations. (cdc.gov)
  • Exploring the global landscape of genetic variation in coagulation factor XI deficiency. (cdc.gov)
  • The effects of highly purified pharmacological grade hyaluronan of defined molecular weights 11, 52, 87, 250 and 970 kilodaltons were tested on mouse macrophage cell lines RAW 264.7 and MHS. (nel.edu)
  • In the present study, we analyzed the effects of length on the interaction between glass fibers and macrophages by focusing on production of the inflammatory cytokine tumor necrosis factor (TNF)-alpha in a mouse macrophage cell line (RAW 264.7). (cdc.gov)
  • Little expression of IL-1 alpha, IL-1 beta, and TNF genes was observed in normal ICB. (nih.gov)
  • Expression of IL-1 beta and TNF genes showed a pattern similar to that of IL-1 alpha. (nih.gov)
  • Expression of IL-1 alpha, IL-1 beta, and TNF genes was dramatically up-regulated in the process of EIU. (nih.gov)
  • The emergence of candidate disease genes, as well as the advances in mapping out molecular pathways involved in schizophrenia , will likely pave the road to understanding and treating an incredibly serious and debilitating disorder. (health.am)
  • En ese sentido, existe un creciente enfoque en la identificación de genes, polimorfismos genéticos asociados y loci de susceptibilidad múltiples. (scielo.br)
  • La presente revisión destaca los genes y polimorfismos genéticos actualmente estudiados, identificados como influyentes en la génesis de la EI, como los genes MMP-3, IL-6, colágeno tipo 1 y receptores de vitamina D y de estrógeno. (scielo.br)
  • Using human cellular DNA microarrays, we found that many nuclear factor kappa B (NF-kappaB)-responsive genes were down-regulated in VZV-infected fibroblasts, suggesting that VZV infection inhibited the NF-kappaB pathway. (simulations-plus.com)
  • The development of CRC involves a step-by-step accumulation of mutations and epigenetic changes in oncogenes and tumour suppressor genes. (org.sa)
  • Tumor necrosis factor, alpha-induced protein 8 is a protein that in humans is encoded by the TNFAIP8 gene. (wikipedia.org)
  • Scholars@Duke publication: Transcriptional down-regulation of tumor necrosis factor-alpha gene expression by a synthetic peptide homologous to retroviral envelope protein. (duke.edu)
  • We have previously shown that a synthetic peptide (CKS-17) homologous to retroviral envelope protein suppresses the accumulation of superantigen staphylococcal enterotoxin-induced TNF-alpha mRNA in human PBMC and in highly purified human monocytes. (duke.edu)
  • Taken together, these results suggest that the synthetic retroviral peptide CKS-17 down-regulates TNF-alpha mRNA expression through inhibition of transcriptional activation of the TNF-alpha gene, which requires de novo synthesis of a transcriptional repressor protein(s). (duke.edu)
  • For example, the mitogen-activated protein kinase (MAPK) p38 pathway mediates stabilization of tumor necrosis factor alpha (TNF-alpha) mRNA in myeloid cells stimulated with bacterial lipopolysaccharide (LPS). (ed.ac.uk)
  • The zinc finger protein tristetraprolin (TTP) is expressed in response to LPS and regulates the stability of TNF-alpha mRNA. (ed.ac.uk)
  • The p38 pathway is required for the induction of TNF-alpha RNA-binding activity and for the expression of TTP protein and mRNA. (ed.ac.uk)
  • Our findings demonstrate a direct link between a specific signal transduction pathway and a specific RNA-binding protein, both of which are known to regulate TNF-alpha gene expression at a posttranscriptional level. (ed.ac.uk)
  • IkappaBalpha levels did not diminish even though the protein became phosphorylated and ubiquitinated, as determined based on detection of the characteristic high-molecular-weight form of the protein, and the 26S proteasome remained functional in VZV-infected cells. (simulations-plus.com)
  • By means of combinatorial protein engineering and protein library technology, smaller antibody fragments or alternative non-immunoglobulin protein scaffolds can be engineered for various functions based on molecular recognition. (avhandlingar.se)
  • The results in paper II show how protein engineering, in vitro display and multi-parameter fluorescence-activated cell sorting can be used to accomplish the challenging task of incorporating two high affinity binding-sites, for albumin and tumor necrosis factor-alpha, into this new bispecific protein scaffold. (avhandlingar.se)
  • Macrophage Inflammatory Protein-3 alpha (MIP-3a), also known as C-C Motif Chemokine Ligand 20 (CCL20) is a 70 amino acid chemokine (molecular weight 8.0 kDa). (quanterix.com)
  • Recombinant TNF-alpha is a disulfide-linked trimeric protein,consisting of 157 amino acids and migrates as an approximately 18 kDa protein under reducing conditions. (reprokine.com)
  • F7 gene variants modulate protein levels in a large cohort of patients with factor VII deficiency. (cdc.gov)
  • Magnetic Luminex Assay Kit for Tumor Necrosis Factor Alpha (TNFa) ,etc. (uscnk.com)
  • Tumor necrosis factor alpha antagonists (TNFA) are biological agents to treat chronic inflammatory and autoimmune diseases. (urosario.edu.co)
  • Tamura K , Dudley J , Nei M , Kumar S . MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. (cdc.gov)
  • Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. (lu.se)
  • Resveratrol shows anti-tumour activities in an in vitro in various tumour types including ovarian cancer. (otago.ac.nz)
  • In vitro, ivacaftor does not alter LPS-induced CD80 and tumor necrosis factor alpha mRNA increases in BV2 microglial cells, while augmenting mRNA levels of these markers in mouse macrophages and differentiated human THP-1-derived macrophages. (lu.se)
  • The pathogenetic mechanism of inherited lipodystrophies at the molecular level has been linked to mutations of lamin A/C, peroxisome proliferator-activated receptor (PPAR-gamma), and other seemingly unrelated proteins. (medscape.com)
  • The activation of this pathway causes a cellular antiviral response, including the production of alpha/beta interferon, cytokines, and other proteins that restrict viral infection. (simulations-plus.com)
  • The engineered binding proteins generated against the epidermal growth factor receptors 2 and 3 in papers III and IV are aimed in this direction. (avhandlingar.se)
  • Serum amyloid A and alpha-1 acid glycoprotein are transport proteins, and fibrinogen is a coagulation factor. (msdmanuals.com)
  • These antigens include organic dusts containing bacteria, fungi,4 animal or plant proteins, or low-molecular-weight chemicals. (cdc.gov)
  • Molecular Immunology. (wikipedia.org)
  • WALTHAM, MA, June 24, 2021 / B3C newswire / -- Scipher Medicine , a precision immunology company matching patients with the most effective therapy, today announced positive results from a multicenter, blinded prospective observational clinical study assessing the ability of the PrismRA test to predict which patients with rheumatoid arthritis are unlikely to respond to tumor necrosis factor-alpha inhibitor (TNFi) therapies either when prescribing initial targeted therapy or when modifying treatment. (b3cnewswire.com)
  • CIBERSORT and TIMER algorithms confirmed that there are significant differences in tumor-infiltrating immune cells in different risk groups. (techscience.com)
  • Receptor tyrosine kinases including EGFR, HER-2 and c-Met and non receptor tyrosine kinase including Jaks are selectively expressed in primary tissue and metastatic tumour of advanced ovarian cancer cells. (otago.ac.nz)
  • Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. (thermofisher.com)
  • Mouse spleen lymphocytes became cytotoxic to tumor cells after culture with AG and FU at concentrations of 10-100 μg/mL. (elsevierpure.com)
  • Optimized DNA sequence encoding Human Tumor Necrosis Factor-alpha mature chain was expressed in CHO Cells. (reprokine.com)
  • within a given tumor, cells may be variably arranged in broad sheets, nests, trabeculae, true papillae, or pseudopapillae (see the first 3 images below). (medscape.com)
  • Another characteristic architectural feature found in many of these tumors is a pseudoalveolar pattern in which cells are arranged in alveolae with central cellular discohesion (see the fourth image below). (medscape.com)
  • Psammoma bodies and scattered xanthoma cells have been described in some tumors. (medscape.com)
  • at least focally, tumor cells often have abundant clear cytoplasm, mimicking clear cell RCC. (medscape.com)
  • In Xp11.2 translocation-associated renal cell carcinoma, tumor cells with abundant eosinophilic cytoplasm and high nuclear grade are often arranged in large nests with a delicate, intervening vascular stroma. (medscape.com)
  • The nests of tumor cells in Xp11.2 translocation-associated renal cell carcinomas may become centrally discohesive, giving rise to a pseudoalveolar growth pattern. (medscape.com)
  • Although related treatment has improved, there are still challenges in combating tumor heterogeneity due to prognostic prediction difficulty ( Eisenstein, 2015 ). (techscience.com)
  • Fekade D , Knox K , Hussein K , Melka A , Lalloo DG , Coxon RE , Prevention of Jarisch-Herxheimer reactions by treatment with antibodies against tumor necrosis factor alpha. (cdc.gov)
  • Isotypic analysis of antibodies against activated Factor VII in patients with Factor VII deficiency using the x-MAP technology. (cdc.gov)
  • Molecular markers have shown excellent prognostic performance with the development of genome sequencing technology. (techscience.com)
  • Large scale multi-institutional and multiregional translational studies are required to generate molecular data that would inform diagnostic, prognostic, and risk-stratification guidelines for Saudi CRC patients. (org.sa)
  • The molecular pathology of cancer has improved our understanding of carcinogenesis, and has enabled the discovery of diagnostic, prognostic, and predictive molecular markers of clinical utility. (org.sa)
  • Nuclear run-on transcription assays further reveal that CKS-17 suppresses the TNF-alpha mRNA transcription rate. (duke.edu)
  • Insulin resistance states are associated with endothelial dysfunction, but the molecular mechanisms underlying this association are incompletely understood. (endocrine-abstracts.org)
  • 2021 ). They exert multiple biological functions, including limiting excessive inflammatory responses, regulating metabolism and immune cell functions, decreasing the production of pro-inflammatory factors, increasing anti-inflammatory mediators, and promoting tissue repair and homeostasis (Miao et al. (springer.com)
  • TB incidence partially rebounded in 2021, which might be explained by a lessening of the effects of the factors associated with the pandemic, as well as delayed detection of cases with symptom onset during 2020 that were not diagnosed until 2021 because of delayed healthcare access or missed diagnoses. (cdc.gov)
  • The present study was designed to examine the underlying mechanism(s) by which CKS-17 down-regulates the TNF-alpha mRNA expression using a human acute monocytic leukemia cell line THP-1 stimulated with the superantigen staphylococcal enterotoxin E. A cyclooxygenase inhibitor indomethacin does not reverse the inhibition of TNF-alpha mRNA expression by CKS-17, suggesting that prostaglandins are not responsible for the suppressive action of CKS-17. (duke.edu)
  • Association of B-cell activating factor receptor deficiency with the P21R polymorphism and common variable immunodeficiency. (cdc.gov)
  • Extracellular Domain Of The 55Kda Tumor Necrosis Factor Receptor. (lu.se)
  • Crystallographic evidence for dimerization of unliganded tumor necrosis factor receptor. (lu.se)
  • The inhibition of both TNF-alpha production and NF-kappaB activation by N-acetyl-L-cysteine, an antioxidant, indicates that generation of oxidants may contribute to the induction of this cytokine and activation of this transcription factor by glass fibers. (cdc.gov)
  • Tumor necrosis factor-alpha (TNF-alpha), a pivotal pro-inflammatory cytokine in RA may be involved in the development of the disturbed lipid metabolism. (researchgate.net)
  • Nuclear factor erythroid-2-related factor 2 (Nrf2) is a transcription factor that is upregulated in times of oxidative stress. (hindawi.com)
  • Correspondingly, hyaluronan of any tested molecular weight did not stimulate nuclear factor kappa B activation. (nel.edu)
  • Activation of the transcription factor nuclear factor (NF)-kappaB was responsible for this induced promoter activity. (cdc.gov)
  • Within any tumor, nuclear size may vary, but uniformly, these tumors are of a high Fuhrman nuclear grade. (medscape.com)
  • abstract = "Background Tumor necrosis factor-alpha (TNF-α) has been shown to be implicated in both muscle regeneration and muscle wasting. (iucc.ac.il)
  • In contradiction, the biological effects of fragmented low molecular weight hyaluronan are suggested to be pro-angiogenic and pro-inflammatory. (nel.edu)
  • Interestingly, the data does not support the current view of low molecular weight hyaluronan as a pro-inflammatory mediator for macrophages. (nel.edu)
  • The plasma levels of inflammatory factors such as TNF-α, IL-1β and IL-6 were raised in perioperative period. (researchsquare.com)
  • MIP-3a is upregulated by inflammatory cytokines such Tumor Necrosis Factor Alpha (TNFα) and Interferon Gamma (IFNγ) while being strongly down regulated by IL-10. (quanterix.com)
  • [ 3 ] In cases linked to injections of insulin, an immune response mediated by tumor necrosis factor-alpha has been hypothesized. (medscape.com)
  • Cellular Components of the Immune System The immune system consists of cellular components and molecular components that work together to destroy antigens. (msdmanuals.com)
  • My research interests are focusing on the activity of tyrosine kinases, targeted inhibitors, effects of ascitic fluid ,and anti-tumour properties of natural food compounds in the progression of advanced ovarian cancer. (otago.ac.nz)
  • RTOG 0417 was a development II study of 49 patients treated with bevacizumab in combination with concurrent radiotherapy and cisplatin in stage IIBВ-IIIB infirmity or IBВ-IIA disease with biopsy-proven pelvic nodal metastasis and/or tumor size of at least 5 cm [53]. (daubnet.com)
  • The mRNA stability assays using actinomycin D show that CKS-17 does not decrease the TNF-alpha mRNA stability. (duke.edu)
  • Proposed molecular mechanisms of oxidative stress-induced Nrf2 activation. (hindawi.com)
  • The underlying molecular mechanisms controlling TNF-alpha production were investigated at the gene transcription level. (cdc.gov)
  • The effect of different molecular weight hyaluronan on macrophage physiology. (nel.edu)
  • Krejcova D, Pekarova M, Safrankova B, Kubala L. The effect of different molecular weight hyaluronan on macrophage physiology. (nel.edu)
  • This review details the anatomy, development, physiology, and molecular characteristics of the Lemnaceae to introduce them to the broader plant research community. (osti.gov)
  • Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. (novusbio.com)
  • To localize and determine the levels of interleukin-1 alpha (IL-1 alpha), interleukin-1 beta (IL-1 beta), and tumor necrosis factor (TNF) gene expression in the process of endotoxin-induced uveitis (EIU) in rats. (nih.gov)
  • Macrophages treated with AG and FU (10-100 μg/mL) exhibited induced tumoricidal activity and increased phagocytosis, lysosomal enzyme activity, and production of nitrite, H 2 O 2 , tumor necrosis factor (TNF)-α, and interleukin (IL)-6. (elsevierpure.com)
  • Macrophages treated with AG and FU (10-100 μg/mL) exhibited induced tumoricidal activity and increased phagocytosis, lysosomal enzyme activity, and production of nitrite, H2O2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6. (elsevierpure.com)
  • Similarly, hyaluronan of any molecular weight neither exerted stimulatory nor inhibitory effects on macrophages pre-treated by lipopolysaccharide. (nel.edu)
  • Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. (novusbio.com)
  • Native human Tumor Necrosis Factor-alpha is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 18 kDa. (reprokine.com)
  • FoxO4 Regulates Tumor Necrosis Factor Alpha-Directed Smooth Muscle Cell Migration by Activating Matrix Metalloproteinase 9 Gene Transcription. (knu.ac.kr)
  • We show that stimulation of RAW264.7 mouse macrophages with LPS induces the binding of TTP to the TNF-alpha 3' untranslated region. (ed.ac.uk)
  • Critical role of glass fiber length in TNF-alpha production and transcription factor activation in macrophages. (cdc.gov)
  • The results show that glass fibers induced TNF-alpha production in macrophages and that this induction was associated with activation of the gene promoter. (cdc.gov)
  • Translocation carcinoma is a renal cell carcinoma (RCC) subtype that harbors a translocation involving a member of the microphthalmia transcription factor gene family. (medscape.com)
  • It has been reported that BBB disruption could be induced by many factors, including neuroimflammation, oxidative stress, and hypoxia [ 9-11 ]. (researchsquare.com)
  • Long fibers (17 micrometer) were significantly more potent than short fibers (7 micrometer) in inducing NF-kappaB activation, the gene promoter activity, and the production of TNF-alpha. (cdc.gov)
  • Further studies are necessary to clarify the effects of different molecular weight hyaluronan on phagocytes. (nel.edu)
  • Chayote has a high- (340 kDa) and a low- (46 kDa) molecular weight (MW) polysaccharide fraction containing high amounts of galactose, arabinose and galacturonic acid. (diva-portal.org)
  • MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. (thermofisher.com)
  • Molecular basis of complement factor I deficiency in Tunisian atypical haemolytic and uraemic syndrome patients. (cdc.gov)
  • RÉSUMÉ La présente étude a examiné les cellules souches hématopoïétiques de 19 cas de syndrome myélodysplasique de haut risque à la recherche de signaux apoptotiques et anti-apoptotiques et de proliférations cellulaires et a établi un lien entre ces derniers et les sous-types cytogénétiques et cliniques, en particulier la trisomie 8. (who.int)
  • Les cas de syndrome myélodysplasique de haut risque avaient un pourcentage nettement supérieur de cellules CD34+ apoptotiques et de survivine+ anti- apoptotiques par rapport aux témoins, en particulier dans les cas atteints de trisomie 8. (who.int)
  • Molecular docking analysis of two bioactive molecules KLUF10 and KLUF13 isolated from the marine bacteria Micrococcus sp. (bioinformation.net)
  • The ascitic fluid contains a mixture of cytokines, growth factors, chemokines and bioactive lipid molecules. (otago.ac.nz)
  • Consistent characteristic changes in the pressure pulse wave shape have been associated with ageing, risk factors for cardiovascular disease and impaired NO bioactivity. (endocrine-abstracts.org)
  • VZV infection also inhibited the characteristic degradation of IkappaBalpha that is induced by exposure of fibroblasts to tumor necrosis factor alpha. (simulations-plus.com)
  • PrismRA, a molecular signature test, is a revolutionary advancement bringing precision medicine to the treatment of rheumatoid arthritis, which affects 20 million patients globally. (b3cnewswire.com)
  • To understand these factors, I am using three dimensional (3D) cell cultures, chicken embryos and a syngeneic mouse model to investigate the biological activity in ovarian cancer. (otago.ac.nz)
  • In this review, we discuss the consequences of hypoxia, as well as the molecular and physiological processes that occur in subjects exposed to HBOT. (mdpi.com)
  • This dearth of molecular pathology data is aptly reflected in the paucity of molecular markers recommended for testing by the Saudi National Cancer Centre guidelines for CRC management. (org.sa)
  • Analysis of Phenotype and L12R Mutation in Signal Peptide and 3' Non-translation Region c11814-insAA Mutation of F7 Gene in a Family with Hereditary Coagulation Factor VII Deficiency]. (cdc.gov)
  • We first determined the effects of tumor necrosis factor-α (TNF-α) and IL-10 on monocyte prostaglandin E 2 (PGE 2 ) production. (elsevierpure.com)
  • We first determined the effects of tumor necrosis factor-α (TNF-α) and IL-10 on monocyte prostaglandin E2 (PGE2) production. (elsevierpure.com)