Molecular transgenic adult mammalian cells. Medical search. Frequent questions

These scientists represented disciplines that included molecular biology, molecular genetics, developmental neurobiology, auditory and vestibular morphophysiology, gravitational biology and motor control. (nih.gov)
Sodium chloride is widely used in biochemistry and molecular biology research. (sigmaaldrich.com)
Nuclear transfer is one of the most sophisticated, fickle, and challenging techniques in cell biology. (rupress.org)
The reason I then was drawn into epigenetics and stem cell biology was a lecture at the IMP by Rudolf Jaenisch. (rupress.org)
The Hsieh laboratory is a neural stem cell biology laboratory that focuses on 4 major areas: (1) epilepsy-in-a-dish, (2) 3D cerebral organoids, (3) patient recruitment, and (4) mechanisms of adult neurogenesis. (utsa.edu)
Using transgenic mouse models, cell biology approaches, genomics and proteomic techniques we study the effects of different hormonal stimuli in ontogenesis and function of various organs. (fiu.edu)
Development: For advances in developmental biology and stem cells. (lu.se)

In the last four decades, transgenic and knockout mouse models have helped to understand the mechanisms of mammalian sex determination, germ cell development, and adult gonad functions. (lidsen.com)
In addition, gene identification in patients permits us to identify naturally occurring animal models or create new transgenic or knockout animal models with retinal degeneration due to defects in the gene homologs. (berkeley.edu)
On June 21 and 22, 1999, the National Institute on Deafness and Other Communication Disorders (NIDCD) and the Life Sciences Division of the National Aeronautics and Space Administration (NASA) co-sponsored a program planning workshop, "Role of Transgenic and Knockout Studies in Understanding Sensory-Motor Performance in Altered Gravitational Performance. (nih.gov)
Indeed, levels of ATP-the molecule used to move energy around inside cells-are increased in knockout worms. (elifesciences.org)
We have recently generated a conditional knockout model of Brd2 which will allow us to determine its function in adult tissues, including the germ line. (columbia.edu)
We showed that COG4 knockout led to a decrease of extracellular HS that specifically affected dsRNA transfection efficiency and reduced viral production, which explains the increased cell survival of these mutants.IMPORTANCE When facing a viral infection, the organism has to put in place a number of defense mechanisms in order to clear the pathogen from the cell. (cnrs.fr)
He makes knockout and transgenic mice to study the function of genes in bone development and cancer. (rupress.org)
The strain is now valued as a source of embryonic stem cells for making knockout mice. (jax.org)

In this review, we focused on the molecular mechanisms of ovarian sex determination and on understanding mutual cross-talks between central molecules in sex development which might impact fertility later in adult life. (lidsen.com)
The De Falco lab is interested in uncovering the mechanisms underlying the differentiation of the fetal gonad, focusing on how myeloid cells (such as macrophages) and vasculature promote tissue remodeling during organogenesis. (cincinnatichildrens.org)
The scientific presentations emphasized developmental processes, encompassing such areas as: 1) the role of homeobox genes and growth factors in inner ear development and plasticity, 2) effects of altered gravitational loading on the development of the inner ear gravity receptor, 3) effects of altered gravitational loading on developing and mature neuromuscular systems and 4) molecular mechanisms that determine muscle fiber diversity and hypertrophic responses. (nih.gov)
While the link between metabolism and aging is well known, the work of Guo and García offers insights into some of the molecular mechanisms that may form the basis of this relationship. (elifesciences.org)
At just 31 years old, Hochedlinger has already worked on therapeutic cloning in a mouse model ( 2 ), reprogramming cancer nuclei ( 3 ), and the molecular mechanisms controlling stem cell pluripotency ( 4 ). (rupress.org)
In contrast, regulatory T cells (Tregs) play a cardinal role in protecting against autoimmune diseases, inflammation and tissue damage 13 through secretion of IL-10 (ref. 14 ) and other mechanisms. (nature.com)
This study reported that Nam reduced CD4 + T cell infiltration and demyelination 24 , but failed to identify the protective mechanisms of action. (nature.com)
Zebrafish Fin: Complex Molecular Interactions and Cellular Mechanisms Guiding Regeneration. (uni-ulm.de)
Alternative splicing of pre-mRNAs is an important means of regulating developmental processes, yet the molecular mechanisms governing alternative splicing in embryonic contexts are just beginning to emerge. (xenbase.org)
The molecular mechanisms that control these fate decisions in SSCs are largely unknown transcriptional programs critical for SSC function. (utsa.edu)
Research is focused on studying the molecular mechanisms involved in the pathogenesis of Vibrio cholerae , the bacterium that causes cholera, and Francisella tularensis , the bacterium that causes tularemia. (utsa.edu)
In this review, our goal is to introduce epigenetics and its different applications in plants, especially in production of transgenic plants, plants tolerate to biotic and abiotic stresses and understanding the mechanisms of gene silencing. (ac.ir)

My third rotation was in the Weatherall Institute of Molecular Medicine, with Professor Roger Patient. (ox.ac.uk)

An error during these developmental steps in females may lead to defective gonads, affecting the differentiation and/or function of the gonads and the development, differentiation, and maturity of the germ cells. (lidsen.com)
Additionally, we are investigating the roles of myeloid cells in regulating spermatogonial stem cell differentiation in the adult testis. (cincinnatichildrens.org)
It is therefore important to understand the molecular signals, which govern the proliferation, migration, and differentiation of endogenous NSCs in the neurogenic niches. (uni-marburg.de)
For the analysis of differentiation and survival of newly built cells, BrdU was administered intraperitoneally on 5 consecutive days once per day to orexin/ataxin-3 mice (n = 8) and WT mice (n = 8) and they were sacrificed 30 days after the last injection. (uni-marburg.de)
The proportion of newborn neurons among all newly born cells was higher in the HC, however, no significant differences in the differentiation of newly built cells could be found in the OB. (uni-marburg.de)
Genetic control of mammalian germ cell differentiation. (columbia.edu)
A second area of research involves elucidating the function of the BET family of double bromodomain-containing proteins, proteins that read epigenetic marks, during germ cell differentiation and neural development. (columbia.edu)
Finally, the lab is pursuing studies on the role of retinoid signaling during male germ cell differentiation, again in using molecular genetic approaches in the mouse model, and more recently, pharmacologic intervention. (columbia.edu)
Transferring a terminally differentiated cell nucleus into an egg cell that has had its own nucleus removed wipes away the epigenetic marks of differentiation and allows the nucleus once again to code for any cell type. (rupress.org)
We show that NAD + regulates CD4 + T-cell differentiation through tryptophan hydroxylase-1 ( Tph1 ), independently of well-established transcription factors. (nature.com)
Our findings unravel a new pathway orchestrating CD4 + T-cell differentiation and demonstrate that NAD + may serve as a powerful therapeutic agent for the treatment of autoimmune and other diseases. (nature.com)
Although differentiation of naïve CD4 + T cells has been considered to be an irreversible process, accumulating evidence suggests that T cells are much more flexible than initially believed with the ability to interchange between helper phenotypes 15 . (nature.com)
2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
Blocking the differentiation of germinal center B cells is dangerous. (bcl-2inhibitors.com)

molecular genetic analysis of the behavioral and neuroendocrine responses to stress. (cincinnatichildrens.org)
The genetic and biochemical diversity of photoreceptor degnereration presents major challenges for therapy as there are many pathways to cell death. (berkeley.edu)
Recent advances in molecular genetics have greatly enhanced the search for the genetic determinants of complex biobehavioral functions. (nih.gov)
The research interests of the Wolgemuth lab focus on understanding the genetic control of gametogenesis and embryogenesis using mouse models and gene targeting, transgenic, and molecular and cell biological approaches. (columbia.edu)
In addition to basic science investigations in such areas as tumor cell invasion, oncogenes, immunotherapy and genetic inheritance, our scientists and physicians participate in national cancer trials. (upstate.edu)
During the last five years, enormous progress has been made in understanding the molecular basis of circadian systems, mainly by molecular genetic studies using the mouse and fly. (bioone.org)
Agoulnik's work has focused on using various cell-based, genetic, and transgenic technology to uncover the functions of various genes in normal development and in diseases. (fiu.edu)
In our research we combine a series of well-established behavioral tests to morphological and neuroanatomical approaches, including histological/anatomical and confocal imaging techniques and brain surgery, coupled to genetic tools using viral vectors and transgenic mouse lines. (unito.it)
Here, we leverage a set of genome-edited human PLIN2 reporter cell lines in a series of CRISPR-Cas9 loss-of-function screens, identifying genetic modifiers that influence PLIN2 expression and post-translational stability under different metabolic conditions and in different cell types. (stanford.edu)

The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
To date, most gene therapies have targeted monogenic recessive retinal diseases and employed viral vectors to transfer a 'normal ' copy of the mutated gene to the affected cell. (berkeley.edu)
In particular, for retinal gene therapy it would be highly advantageous to transduce a single cell type that spans the entire retina after an intravitreal injection of a gene delivery vehicle for the subsequent secretion of a general neuroprotective factor throughout the retina. (berkeley.edu)
The postulated pathophysiology of human narcolepsy is mimicked very closely in a transgenic mouse model called orexin/ataxin-3 mouse, in which orexin-containing neurons are ablated progressively by specific overexpression of a truncated cytotoxic ataxin-3 gene product under the orexin-promoter. (uni-marburg.de)
I also designed and constructed sgRNAs in preparation for the use of the CRISPR/Cas9 system to target the CD40 gene in human induced pluripotent stem cells as a rational approach to the production of tolerogenic dendritic cells. (ox.ac.uk)
Thus, to examine the spatial generation of functional HSCs within the mouse embryo, we used the well-known HSC marker, Sca-1, and a transgenic approach with an Ly-6A (Sca-1) GFP marker gene. (ox.ac.uk)
At the molecular level, knockdown of PQBP1 in Xenopus animal cap explants inhibits target gene induction by FGF but not by BMP, Nodal or Wnt ligands, and knockdown of either PQBP1 or WBP11 in embryos inhibits expression of fgf4 and FGF4 -responsive cdx4 genes. (xenbase.org)
Epigenetic regulation is necessary for the production of differentiated cells throughout plant development, as well as maintaining the stability and integrity of the gene expression profiles. (ac.ir)
We examined the contribution of Fxr1 to cell-autonomous homeostatic synaptic scaling and neuronal responses to sleep loss, using a combination of gene overexpression and Crispr/Cas9-mediated somatic knockouts to modulate gene expression. (bvsalud.org)

Genetics and Molecular Research: GMR, 15:15018299. (ac.ir)

This allowed us to determine that the heparan sulfate pathway and the conserved oligomeric Golgi complex are key determinants allowing entry of both dsRNA and viral nucleic acid leading to cell death. (cnrs.fr)
Viral titers in ferret nasal wash specimens and nasal turbinate tissue correlated positively with peak titer in Calu-3 cells, whereas additional phenotypic and molecular determinants of influenza virus virulence and transmissibility in ferrets varied in their association with in vitro viral titer measurements. (cdc.gov)

Stem cell-like transcriptional reprogramming mediates metastatic resistance to mTOR inhibition. (rochester.edu)
PR-domain-containing Mds1-Evi1 is critical for long-term hematopoietic stem cell function. (rochester.edu)
Introduction: The adult mammalian brain retains neural stem cells (NSCs) that continually generate new neurons within two restricted regions, the subventricular zone (SVZ) of the lateral ventricle and the subgranular zone (SGZ) of the dentate gyrus in the hippocampus (HC). (uni-marburg.de)
For the evaluation of the stem cell proliferation in SVZ and SGZ and migration in the RMS, orexin/ataxin-3 mice (n = 8) and wild type (WT) mice (n = 8) received a single intraperitoneal injection of 100 mg BrdU (bromodeoxyuridine)/kg body weight 2 hour prior to sacrifice. (uni-marburg.de)
Results: We found a significantly higher proliferation of stem/precursor cells in the orexin/ataxin-3 mice in both neurogenic regions, the SGZ and the SVZ. (uni-marburg.de)
Conclusion: In the absence of orexin in the adult mouse brain, the proliferation of adult neural stem/precursor cells was increased, the survival rate was significantly increased in the granular zone of the OB and a consistent not significant trend was seen in the HC. (uni-marburg.de)
We have demonstrated that cyclin A1 is essential for the progression of spermatocytes into the first meiotic division, that cyclin A2 is required for mitotic divisions of male germ cell stem cells, and that the E-type cyclins have unexpected function during meiosis, notably in the maintenance of telomere integrity. (columbia.edu)
I. Demonstration of monoamines in cell bodies of brain stem neurons. (nature.com)
They used adult cells, but it was possible that the cells that gave rise to successfully cloned animals were derived from rare adult stem cells. (rupress.org)
The aim of this project was to investigate a potential means to induce tolerance in dendritic cells derived from induced pluripotent stem cells to assist in the treatment of the lysosomal storage disease, Pompe disease. (ox.ac.uk)
Hematopoietic stem cells localize to the endothelial cell layer in the midgestation mouse aorta. (ox.ac.uk)
The emergence of the first adult hematopoietic stem cells (HSCs) during mammalian ontogeny has been under intense investigation. (ox.ac.uk)
Stem Cell Res. (sciendo.com)
The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
Stem Cells Transl. (sciendo.com)
Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
Adipose stem cells in reparative goat mastitis mammary gland. (sciendo.com)
Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
Given the tremendous promise of regenerative medicine to enhance human health and treat disease, Congress included a provision in the 21st Century Cures Act to support a Regenerative Medicine Innovation Project ($30 million distributed over FY17 through FY20) for the funding of clinical research to further the field of regenerative medicine (RM) using adult stem cells, including autologous, non-autologous use as well as eligible induced pluripotent stem cells (iPSC). (nih.gov)
Student projects in my lab will revolve around cell-fate decisions in Spermatogonial Stem Cells (SSC). (utsa.edu)
SSCs are adult-tissue stem cells in the mammalian testis that balance self renewing and differentiating fate decisions to give rise to and sustain the entire spermatogenic lineage. (utsa.edu)
Our studies contributed to the definition of the morphological, molecular and functional features of postnatal/adult neurogenic zones, including neural stem cell niches and parenchymal progenitors, in brain homeostasis and in animal models of lesion/neurodegeneration. (unito.it)
Stem Cell Reports. (lu.se)

We have gained crucial insights into molecular factors and pathways of the cells generating either the supporting gonadal cells or germ cells of both sexes. (lidsen.com)
These studies have served to highlight the role of AND-34/BCAR3 and p130Cas in cell adhesion and migration signaling pathways. (molvis.org)
Symptoms seen with choroid plexus tumors generally result from blocking of the CSF pathways and debatably by secretion of CSF by tumor cells, both leading to increased fluid and, eventually, to hydrocephalus . (medscape.com)

For example, the identification of regulatory sequences of genes expressed specifically in the vestibular system will allow the targeted introduction of foreign genes into the vestibular system of transgenic mice. (nih.gov)
Long-term efforts of this project will involve developing RAR-alpha selective antagonists, identifying the target genes of RAR-alpha, and determining their function in germ cell-Sertoli cell interactions. (columbia.edu)
In this study, we employed a genome-wide CRISPR-Cas9 loss-of-function screen based on cell survival in order to identify genes implicated in the host response to dsRNA. (cnrs.fr)
Using a genome-wide screening approach, we set to identify genes involved in dsRNA entry, sensing, and apoptosis induction in human cells. (cnrs.fr)
A second strategy will also be employed: Using ATAC-Seq data from cardiomyocytes taken from both embryonic hearts and adult injured hearts, it will be possible to investigate putative enhancers of gata4 , 5 and 6 genes in the developing embryo and the regenerating heart. (ox.ac.uk)
For example, cerebellar granule cells express six subunit genes abundantly (α 1 , α 6 , β 2 , β 3 , γ 2 , and δ), and so they probably have several distinct GABA A receptor subtypes of unknown subunit stoichiometry. (jneurosci.org)
Extensive evidence has revealed that the core clock machinery involves "clock genes" and "clock proteins" functioning as molecular cogs. (bioone.org)
This mechanism enables differentiated cells in a multicellular organism to express only the genes which are necessary for their own activity. (ac.ir)
We generated hCOs from 17 patients and controls, profiling 167,958 cells with single-cell RNA-sequencing analysis, which revealed neuronal-specific differential expression of genes outside the 16p11.2 region that are related to cell-cell adhesion, neuronal projection growth, and neurodevelopmental disorders. (bvsalud.org)

By triple staining we could show that the proportion of newly born neurons relative to the total number of newly built cells in the HC was significantly higher with 90 % in the orexin-ataxin group compared to 83 % in the control group. (uni-marburg.de)
In both the granular zone and the periglomerular zone of the OB, over 90 % of the total amount of new built cells differentiated into neurons in both groups. (uni-marburg.de)
These neuroendocrine dopamine cells may contribute to the dopaminergic inhibition of prolactin secretion diurnally, as their neuromedin S + inputs originate from neurons expressing Per2 and Per3 and their tyrosine hydroxylase phosphorylation is regulated in a circadian fashion. (nature.com)
Figure 5: Molecular interrogation of dopamine neurons defines a onecut-3-expressing periventricular subtype. (nature.com)
Because in α 6 −/− neurons the remaining α 1 , β 2/3 , and γ 2 subunits cannot rescue the δ subunit, certain potential subunit combinations may not be found in wild-type cells. (jneurosci.org)
The chief inhibitory neurons of the mammalian brain, GABAergic neurons, are comprised of a myriad of diverse neuronal subtypes. (jneurosci.org)
To facilitate the study of these neurons, transgenic mice were generated that express enhanced green fluorescent protein (EGFP) in subpopulations of GABAergic neurons. (jneurosci.org)
The microanatomical features of these cells suggest that they function primarily as "input-biasing" neurons, in that synaptic volleys in stratum radiatum would lead to their activation, which in turn would result in selective suppression of excitatory input from the entorhinal cortex onto CA1 pyramidal cells. (jneurosci.org)
GABAergic neurons, the chief inhibitory neurons of the mammalian brain, are comprised of diverse neuronal subtypes distinguishable on the bases of location in given brain regions, microanatomical features, including dendritic arborizations and axonal projections, expression of specific macromolecules, and neurophysiological properties. (jneurosci.org)
Our research interest is focused on the study of these neurogenic niches by tackling different aspects of the process that govern the generation and integration of new neurons into pre-existing neuronal circuits in the adult brain. (unito.it)
We are also interested in unravel the potential role of adult-born neurons in olfactory sensory processing with particular attention to olfactory experiences that are behaviourally relevant. (unito.it)
We used a CRISPR-Cas9-mediated intersectional approach to achieve targeted deletion of GSK3ß in D2-expressing neurons of the adult medial prefrontal cortex (mPFC). (bvsalud.org)

Inherited forms of retinal degeneration, which afflict 1 in 3000 people worldwide, arise primarily from mutations in transcripts expressed in rod and cone photoreceptors and retinal pigment epithelial cells. (berkeley.edu)
Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. (molvis.org)
Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
While Akt Ser 473 phosphorylation was readily detectable in AND-34 +/+ lens epithelial cells, it was markedly reduced in the AND-34 −/− lens epithelium. (molvis.org)
These results demonstrate the loss of AND-34 dysregulates focal adhesion complex signaling in lens epithelial cells and suggest that AND-34-mediated signaling is required for maintenance of the structural integrity of the adult ocular lens. (molvis.org)
Cataracts are the leading cause of blindness worldwide and are characterized by progressive aggregation and precipitation of lens proteins, and the development of age‑related cataracts is associated with dysregulated cellular activities of lens epithelial cells. (spandidos-publications.com)
A luciferase assay demonstrated that transfection of lens epithelial cells with wild type TP53 3'‑UTR significantly reduced the luciferase activity of the miR‑125b overexpressing cells compared with scramble controls. (spandidos-publications.com)
Furthermore, it was demonstrated that the expression level of miR‑125 was comparable in epithelial cells from patients with age‑associated cataracts and controls, whereas the expression level of TP53 was significantly higher in the cataract group compared with controls. (spandidos-publications.com)
Additionally, transfection with 50 nM miR‑125b mimics markedly reduced the mRNA and protein expression levels of TP53 in the cultured lens epithelial cells, and miR‑125b significantly induced apoptosis in the epithelial cells compared with negative control cells. (spandidos-publications.com)
We compiled data from ferrets inoculated with an extensive panel of over 50 human and zoonotic IAV (inclusive of swine-origin and high- and low-pathogenicity avian influenza viruses associated with human infection) under a consistent protocol, with all viruses concurrently tested in a human bronchial epithelial cell line (Calu-3). (cdc.gov)
We show that certain viral titer measurements following infection of a human bronchial epithelial cell line are positively correlated with viral titers in specimens collected from virus-inoculated ferrets and employ mathematical modeling to identify commonalities between viral infection progression between both models. (cdc.gov)

Current projects in our lab involve development of retinal cell specific viral vectors based upon lentivirus and adeno-associated viruses. (berkeley.edu)
Synthetic dsRNA is also able to mimic viral-induced activation of innate immune response and cell death. (cnrs.fr)
While studies of peptide and protein antigens have been facilitated by the rapid advances in genomics and proteomics, studies of sugar chains, which are abundantly expressed on the outer surfaces of viral, bacterial, protozoan, and fungal pathogens and on the membranes of mammalian cells, have not kept pace with technologic advances. (cdc.gov)

The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. (wikipedia.org)
To highlight some adverse reproductive outcomes associated with perturbations at the molecular and cellular levels in human folliculogenesis, two examples, i.e. (lidsen.com)
Among those molecular cues, double-stranded RNA (dsRNA) is a very potent inducer of different reactions at the cellular level that can ultimately lead to cell death. (cnrs.fr)
Cellular immunology, T cell immunity, autoimmune diseases: The immune system plays a fundamental role in the defense against microbial pathogens. (utsa.edu)

Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
These participate in transcriptional/translational feedback loops and many homologous clock-components in the fruit fly Drosophila are also expressed in mammalian clock tissues with circadian rhythms. (bioone.org)

The retina is a complex tissue in the back of the eye that contains the rod and cone photoreceptor cells. (berkeley.edu)

Whereas a traditional view of GABAergic interneuronal function has been that of performing feedback inhibition, it has become increasingly evident that these cells perform much more complex functions that are crucial to controlling overall brain activity. (jneurosci.org)

Such a review cannot be comprehensive due to space constraints and I focused on the commitment of the female cell lineage and avoided discussing the male cell lineage. (lidsen.com)

In the adult mammalian brain few neurogenic niches that include the main and accessory olfactory bulb and the hippocampus, are maintained. (unito.it)

Adult neurogenesis in the orex. (uni-marburg.de)
This process is called adult neurogenesis. (uni-marburg.de)
However, our knowledge is still insufficient to exploit adult neurogenesis for controlled brain repair or for stimulation of its physiological function. (uni-marburg.de)
Aim: We aimed to study the possible role of the protein orexin in the regulation of adult neurogenesis in the orexin/ataxin-3 mouse. (uni-marburg.de)
Study design: Adult neurogenesis in the SGZ, SVZ and rostral migratory stream (RMS), where the cells migrate from the SVZ to the olfactory bulb (OB), was studied by immunohistology. (uni-marburg.de)

This laboratory pursues several lines of investigation to understand how T cells contribute to autoimmune diseases and protection from infection, and how to modulate T cell immunity for therapeutic purposes in humans. (utsa.edu)
George D. Snell of The Jackson Laboratory won the 1980 Nobel Prize in Physiology or Medicine for "discoveries concerning genetically determined structures on the cell surface that regulate immunological reactions. (jax.org)
The Kopito laboratory seeks a molecular understanding of how cells maintain the fidelity of their proteomes. (stanford.edu)

The generation of mutant and transgenic animal models, involving such species as the mouse, Drosophila, zebrafish and Caenorhabditis elegans (C. elegans), provides powerful tools for elucidation of these functions. (nih.gov)

A number of presentations were focused on the role of CD1 proteins, which present lipid antigens (e.g., from mycobacteria or Francisella tularensis , a potential weapon of bioterrorism) to T cells. (cdc.gov)
The ER is the 'port of entry' for proteins destined for the cell surface and beyond. (stanford.edu)
This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)

We have identified anti-cancer effects of Huaier, and its unique characteristics of 1) no side effects or toxicity, 2) in dose dependent manner, 3) causing specific cancer cell death with simultaneous tissue regeneration, 4) miRNA-mediated transcriptional control on the rescue of multiple signal transduction, by dependent on individual genomic potential. (fortunejournals.com)

Rats transgenic for Huntington's disease (tgHD51 CAG rats), surviving up to two years, represent an animal model of HD similar to the late-onset form of human disease. (hindawi.com)
We have also developed transgenic rats, expressing dominant rhodopsin mutations. (berkeley.edu)

Therefore, it is urgent to uncover the molecular mechanism(s) underlying the physiological changes, so that certain actions could prevent or postpone the non-optimal modifications that occur during aging. (elifesciences.org)
These findings uncover a role of Fxr1 as a shared signaling hub between cell-autonomous homeostatic plasticity and system-level responses to sleep loss, with potential implications for neuropsychiatric illnesses and treatments. (bvsalud.org)

Despite recent advances in our understanding of the mechanism of these retinopathies, much work remains to get a clear picture of the molecular pathology of each disease. (molvis.org)

I went to the Institute of Molecular Pathology (IMP, Vienna) to do my Master's thesis. (rupress.org)
Dr Jamaspishvili has ongoing research interests in molecular and digital pathology of genitourinary cancers. (upstate.edu)

It has previously been demonstrated in Xenopus that gata6 overexpression holds embryonic myocardial cells in a precursor state, but once normal levels are resumed, development continues, but the myocardium is thicker. (ox.ac.uk)
Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)

In addition, flow cytometry analysis and the MTT assay were conducted to detect the effects of miR‑125b on apoptosis and cell viability. (spandidos-publications.com)

Wnt/β-catenin signaling acts cell-autonomously to promote cardiomyocyte regeneration in the zebrafish heart. (uni-ulm.de)

Molecular carcinogenesis. (rochester.edu)
It is extremely required to obtain minimum-essential information to define the molecular basis for cancer recovery, with a comparison to that in the process of carcinogenesis. (fortunejournals.com)

Activating mutations upstream may also underlie some epigenetic or within the ERK1/2 cascade are events that change cell signalling. (who.int)

Other research includes the etiology of germ cell tumors, which are one of the most common tumors arising in children. (cincinnatichildrens.org)
Research at the ASMG lab focuses primarily on the gut microbial composition of ruminants utilizing advanced molecular methodologies. (upenn.edu)
Their research ranges from the biophysics of cell signaling to clinical trials of hormonal contraceptives. (washington.edu)
Recently, research has increased in the area of transplanting embryonic cells across species and growing kidneys and endocrine pancreas cells in situ. (medscape.com)

3] Porcine islet cells of Langerhans have been injected into patients with type 1 diabetes mellitus. (medscape.com)

Here, removal of intact α 6 protein from cerebellar granule cells allowed perturbations in other subunit levels to be studied. (jneurosci.org)

It is as yet unresolved whether these first HSCs are derived from intraembryonic hemangioblasts, hemogenic endothelial cells, or other progenitors. (ox.ac.uk)

Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)

This complicates the interrogation of GSK3ß functions in cortical D2 cells in a circuit-defined manner using conventional animal models. (bvsalud.org)

Although the existence of additional anomalies of the nervous system or other organ systems is difficult to exclude, the existing animal models are a valuable resource for understanding molecular bases for normal inner ear development. (nih.gov)
CD4 + T cells are involved in the development of autoimmunity, including multiple sclerosis (MS). Here we show that nicotinamide adenine dinucleotide (NAD + ) blocks experimental autoimmune encephalomyelitis (EAE), a mouse model of MS, by inducing immune homeostasis through CD4 + IFNγ + IL-10 + T cells and reverses disease progression by restoring tissue integrity via remyelination and neuroregeneration. (nature.com)
Immunoreceptor signaling during development, homeostasis, and effector function of T cells and NK cells. (upstate.edu)

We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)

More recently, the role of Th1 and Th17 CD4 + cells in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), the most commonly used experimental model for human MS, has been challenged, and it has been demonstrated that the transcription factor T-bet (also known as Tbx21 ) is critical for controlling Th1/Th17-mediated autoimmunity 12 . (nature.com)

A metabolomic study of the CRND8 transgenic mouse model of Alzheimer's disease. (neurotree.org)

T lymphocytes for adoptive cell therapy against hematological cancer. (upstate.edu)

Mutant form of huntingtin (mhtt) comprises up to 40 repeats and individuals with 36-39 CAG repeats are in risk of developing adult (late-onset) form of HD. (hindawi.com)
The expanded use of mutant and transgenic animals offers many promising tools for investigating molecular bases of balance function and sensorimotor performance. (nih.gov)
The δ subunit mRNA was present at wild-type levels in the mutant granule cells, indicating a post-translational loss of the δ subunit. (jneurosci.org)

Anatomy61

Organisms26

Diseases3

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes69

Disciplines and Occupations1

Information Science4

Health Care2

Biology7

Knockout8

Mechanisms12

Weatherall Institute of Molec1

Differentiation15

Genetic9

Gene9

Genetics1

Determinants2

Stem25

Pathways3

Genes9

Neurons12

Epithelial11

Viral3

Cellular4

Tissues2

Retina1

Inhibition1

Lineage1

Neurogenic1

Neurogenesis5

Laboratory3

Drosophila1

Proteins3

Transcriptional1

Rats2

Uncover2

Mechanism1

Pathology2

Precursor2

Apoptosis1

Zebrafish heart1

Carcinogenesis2

Mutations1

Research4

Islet cells1

Perturbations1

Endothelial cells1

Mesenchymal2

Interrogation1

Development3

Functional1

Pathogenesis1

Study1

Lymphocytes1

Mutant3