Molecular transcriptional regulation silencer elements transcriptional. Medical search. Frequent questions

Most of these relate to post-transcriptional modification of pre-mRNAs to produce mature mRNA ready for translation into protein. (wikipedia.org)
MicroRNAs perform important roles in the post-transcriptional regulation of gene expression. (researchsquare.com)
A number of post-transcriptional mechanisms fine-tune the gene expression landscape. (researchsquare.com)
Consequently, motifs are indispensable for temporal, spatial, conditional and basal regulation at the transcriptional, post-transcriptional and post-translational level. (biomedcentral.com)
As splicing is linked to transcriptional and post-transcriptional steps, SR proteins are implicated in the regulation of multiple aspects of the gene expression program. (molcells.org)
I will also discuss their emerging regulatory roles in mediating and connecting post-transcriptional processes from the nucleus to the cytoplasm. (molcells.org)

Dr. Benyajati is interested in studying the molecular mechanisms that regulate differential gene expression during development using the Drosophila alcohol dehydrogenase ( Adh ) gene as the model system. (rochester.edu)
Regulation of the Drosophila transcriptome by Pumilio and CCR4-NOT deadenylase. (rochester.edu)
Assays in transgenes in Drosophila previously identified cis -acting transcriptional regulatory elements from homeotic genes. (nih.gov)
This important study advances our understanding of the functions and regulation of the Drosophila transcriptional regulator Bonus, an ortholog of mammalian TIF1 family members. (elifesciences.org)
In Drosophila , the BX-C cluster contains the three most posterior Hox genes, where their collinear activation incorporates progressive replacement of histone modifications, reorganization of 3D chromatin architecture and sequential activation of boundary elements and cis -regulatory regions. (biorxiv.org)

These sequence regions can either be next to the transcribed region (the promoter) or separated by many kilobases (enhancers and silencers). (wikipedia.org)
Enhancers and silencers may be distantly located from the gene, many thousands of base pairs away. (wikipedia.org)
Enhancers are Cis DNA elements that facilitate or enhance initiation of transcription at the promoter, they interact with gene regulatory proteins or trans-factors (so termed because they are produced by other genes ) and increase the rate of expression. (online-sciences.com)
Using chromosome conformation capture, it is now possible to analyze in detail the interaction between enhancers, silencers, boundary elements and promoters at individual loci, but these techniques are not readily scalable. (unito.it)

The sequences and lengths of these elements vary, but the same general functions are present in most genes. (wikipedia.org)
Genes may be regulated by multiple enhancer and silencer sequences that further modify the activity of promoters by binding activator or repressor proteins. (wikipedia.org)
For genes with multiple regulatory sequences, the rate of transcription is the product of all of the elements combined. (wikipedia.org)
Current research goals are to investigate the mechanisms by which interactions of cis -regulatory sequences with sequence-specific trans-acting proteins in situ in chromatin effect the chromatin remodeling and the transcriptional regulation of Adh . (rochester.edu)
Second, systematic examination of transcriptional regulation has yielded new understanding about transcription start sites, including their relationship to specific regulatory sequences and features of chromatin accessibility and histone modification. (kb.se)
Nucleic acid sequences that are involved in the negative regulation of GENETIC TRANSCRIPTION by chromatin silencing. (bvsalud.org)
What are exonic and intronic splicing silencers or enhancer sequences? (pressbooks.pub)

Gene expression regulation is highly connected process. (molcells.org)

The conserved family of Transcription Intermediary Factors (TIF1) proteins consists of key transcriptional regulators that control transcription of target genes by modulating chromatin state. (elifesciences.org)
Mutations in splicing elements, for example, have been found in genes such as LKB1 , KIT , CDH17 , KLF6 and BRCA1 , and changes in trans-acting regulators can affect the expression of genes such as Ron , RAC1 and CD44 . (biologists.com)

Nef protein of HIV-1 is a transcriptional repressor of HIV-1 LTR. (microbiologyresearch.org)
Solid evidence, leveraging both in vivo and in vitro studies, reveals the molecular requirements for Bonus' function as a transcriptional repressor, including a key role for sumoylation. (elifesciences.org)

Molecular Genetics. (rochester.edu)
Genetics and Molecular Research: GMR, 15:15018299. (ac.ir)

Regulatory elements can overlap one another, with a section of DNA able to interact with many competing activators and repressors as well as RNA polymerase. (wikipedia.org)
Eukaryotic genes typically have more regulatory elements to control gene expression compared to prokaryotes. (wikipedia.org)
The murine S100{dollar}\beta{dollar} promoter drives a 4-fold higher level of transcription in C6 rat glioma cells than in non-glial 3T3 murine fibroblast cells, suggesting the existence of a cell type-specific regulatory element within the promoter region. (umaryland.edu)
In order to map the cell type-specific regulatory elements, transcriptional analyses of various deletions of the 5{dollar}\sp\prime{dollar}-flanking region were carried out in both C6 and 3T3 cells. (umaryland.edu)
Two cell type-specific negative regulatory elements, one active in non-glial cells and another active in glial cells, were mapped to the regions {dollar}-{dollar}1552/{dollar}-{dollar}1234 and {dollar}-{dollar}1234/{dollar}-{dollar}551, respectively. (umaryland.edu)
A strong negative regulatory element and a relatively weak negative regulatory element were located in the regions {dollar}-{dollar}551/{dollar}-{dollar}157 and {dollar}-{dollar}1669/{dollar}-{dollar}1552, respectively. (umaryland.edu)
These results lead to the conclusion that the murine S100{dollar}\beta{dollar} gene is under complex transcriptional regulation involving tonic negative control exerted by a combination of multiple cis-acting regulatory elements including cell type-specific elements. (umaryland.edu)
Gel mobility shift assay with the cell type-specific regulatory element III (CSREIII, {dollar}-{dollar}1552/{dollar}-{dollar}1234) showed that a unique trans-acting protein factor may be present in 3T3 nuclear extract but absent in C6 nuclear extract. (umaryland.edu)
Gene expression during development and differentiation is regulated in a cell- and stage-specific manner by complex networks of intergenic and intragenic cis-regulatory elements whose numbers and representation in the genome far exceed those of structural genes. (unito.it)
In addition, we show how Capture-C will expedite identification of the target genes and functional effects of SNPs that are associated with complex diseases, which most frequently lie in intergenic cis-acting regulatory elements. (unito.it)
We find that during normal lifespan of CMs gene regulation is mainly orchestrated by dynamic mCpG and canonical histone marks at distal regulatory and genic regions. (nature.com)
The assays found tissue-specific enhancer elements as well as cis -regulatory elements that are required for the maintenance of activation or repression throughout development. (nih.gov)
While these transgenic assays have been important in defining the structure of the cis -regulatory elements and identifying trans -acting factors that bind to them, their functions within the context of the endogenous genes remain poorly understood. (nih.gov)
Understanding the interactions between TFs and TFBSs provides insights into the complex transcriptional regulatory networks that govern gene expression, with broad implications for several fields such as developmental biology, immunology, and disease research. (bvsalud.org)
Mutations in cis-acting splicing elements or changes in the activity of constitutive or alternative splicing could have a profound regulatory proteins that compromise the accuracy of either impact on human pathogenesis, in particular in tumor development and progression. (biologists.com)
The collinear activation of the Hox genes and their cis -regulatory elements is thought to rely on the progressive opening of the chromatin within the BX-C along the A-P axis. (biorxiv.org)
Finally, we contrast the regulatory properties of protein motifs and the regulatory elements of DNA and (pre-)mRNAs, advocating that co-regulation, co-operativity, and motif-driven regulatory programs are common mechanisms that emerge from the use of simple, evolutionarily plastic regulatory modules. (biomedcentral.com)
transcripts, and known regulatory elements (REs). (biomedcentral.com)
(B) Regulatory elements of the MECP2/Mecp2 gene. (biomedcentral.com)

An additional layer of regulation occurs for protein coding genes after the mRNA has been processed to prepare it for translation to protein. (wikipedia.org)

The extent of dorsal action is closely related to the affinity and the number of dorsal response elements present in these zygotic gene promoters. (silverchair.com)

The structures of both eukaryotic and prokaryotic genes involve several nested sequence elements. (wikipedia.org)
The core promoter of eukaryotic genes typically requires additional activation by promoter elements for expression to occur. (wikipedia.org)
Regulated gene expression is the expression of genes in which the cellular level of their products varies in response to molecular signals, they are called inducible genes or repressible genes . (online-sciences.com)
are the genes which their products decrease in concentration in response to molecular signals, the process of decreasing their expression is called repression, i.e. (online-sciences.com)
The transcriptional silencers are clustered in the two regions whose interactions are required for the maintenance of silencing in the endogenous genes. (nih.gov)
Here, it binds to specific jB elements to drive transcription of target genes [14-20]. (lsd1receptor.com)
About 18% of all C. difficile genes are regulated by glucose, for which 50% depend on CcpA for regulation. (studyres.com)

However, the 5{dollar}\sp\prime{dollar}-flanking region exhibits cell-type specificity when suppressing the S100{dollar}\beta{dollar} promoter-dependent transcription, indicating its involvement in the regulation of the cell type-specific expression of the murine S100{dollar}\beta{dollar} gene. (umaryland.edu)
Rasd1 inhibited transcriptional repression by Ear2 on a renin promoter-luciferase reporter construct both in the presence and absence of all-trans-retinoic acid. (biomedcentral.com)
The EOMES promoter is used as a case study for single DNA sequence analysis, revealing potential regulation by the LHX9-FOXP1 complex during embryonic development. (bvsalud.org)
Understanding the proteins that regulate the 5-HT1A promoter will lead to insights on receptor regulation and provide new therapeutic targets relevant to depression, anxiety, obsessive compulsive disorder and other mental illnesses. (vdocuments.net)
In uninfected and cells immediately post-infection all three DHS sites were open, then the CD3γ promoter became non accessible, and this was followed by a sequential closure of all the DHS sites corresponding to all three transcriptional control regions. (biomedcentral.com)
This chromatin organization differentially instructed alternative Abd-B promoter use, thereby expanding the possibilities to regulate transcriptional output. (biorxiv.org)

In this mini-review, the essential functions of SR proteins in splicing regulation, with a focus on their roles as RNA binders in the ribonucleoprotein complex (RNP) will be summarized. (molcells.org)

American Journal Of Respiratory Cell And Molecular Biology 2023, 68: 75-89. (yalemedicine.org)
EvoRator2: predicting site-specific amino acid substitutions based on protein structural information using deep learning Journal of Molecular Biology: 435(14):168155. (tau.ac.il)
The foamy virus family: molecular biology, epidemiology and neuropathology. (microbiologyresearch.org)

These findings suggest that the glial-specific expression of the murine S100{dollar}\beta{dollar} gene is probably mediated by both cis-acting silencer elements and trans-acting protein factors. (umaryland.edu)
This protein belongs to the rel proto-oncogene and NF-KB transcriptional factor family and acts by controlling zygotic gene expression. (silverchair.com)
Experimental approaches are to characterize individual protein components of the multiprotein complex and to assemble individual chromatin complexes from purified components for structural and transcriptional analysis. (rochester.edu)
Accepted August 23, 2012 ABSTRACT The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. (studyres.com)
Methyl CpG Binding Protein 2 (MeCP2) is a key transcriptional regulator in the brain [ 1 ]. (biomedcentral.com)

To further explore the molecular function of Rasd1, we performed a yeast two-hybrid screen and identified Ear2, a negative regulator of renin transcription, as an interaction partner of Rasd1. (biomedcentral.com)
Together, our work identified Bonus as a regulator of tissue-specific gene expression and revealed the importance of SUMOylation as a regulator of complex formation in the context of transcriptional repression. (elifesciences.org)

While BRCA2 expression is involved in cell cycle checkpoints and DNA repair, the mechanisms of cell cycle-dependent regulation of BRCA2 gene expression remains elusive. (biomedcentral.com)
Recent observations showed that low-molecular-weight compounds might induce L1 retrotransposition through unknown mechanisms. (researchsquare.com)

Characterization of the chromosomal rearrangements shown in Figure 1 also revealed that two genetic elements (proximal and distal MES [maintenance elements for silencing]), about 70 kb apart in the Scr gene, must be in cis to maintain proper repression. (nih.gov)

Third, a more sophisticated view of chromatin structure has emerged, including its inter-relationship with DNA replication and transcriptional regulation. (kb.se)

We used the Crisper/Cas9 methodology to generate chromosomes lacking one, two, or three of the silencing elements. (nih.gov)
Silencing is only disrupted when multiple elements are deleted. (nih.gov)

The rules of gene expression in plants: organ identity and gene body methylation are key factors for regulation of gene expression in Arabidopsis thaliana. (ac.ir)

In addition, in cultured cells twist has been shown to be a transcriptional activator. (silverchair.com)

This study uncovers distinct layers of epigenetic regulation not only during prenatal development and postnatal maturation but also in diseased human cardiac myocytes. (nature.com)
Epigenetic regulation is necessary for the production of differentiated cells throughout plant development, as well as maintaining the stability and integrity of the gene expression profiles. (ac.ir)
Epigenetic Regulation in Plant Responses to the Environment. (ac.ir)
Epigenetic regulation of gene expression is an essential mechanism that guides cell differentiation during development. (elifesciences.org)

The NF-jB family of transcription factors are involved in the regulation of several physiological processes such as inflammation, cell cycle regulation and apoptosis. (lsd1receptor.com)
Cell adhesion and the molecular processes of morphogenesis. (academicinfluence.com)

Individual miRNAs are capable of regulating gene networks by interacting with multiple targets or by suppressing transcriptional controllers [ 6 , 8 ]. (researchsquare.com)
Our findings shed further light on several important signalling pathways involved in lung adenocarcinoma, and suggest new molecular targets for treatment. (kb.se)

A) Basal expression elements consist of the proximal element of the TATA box that direct RNA polymerase II to the correct start site (+1) and the upstream element e.g. (online-sciences.com)
The ease of motif acquisition simplifies access to basal housekeeping functions, facilitates the co-regulation of multiple biomolecules allowing them to respond in a coordinated manner to changes in the cell state, and supports the integration of multiple signals for combinatorial decision-making. (biomedcentral.com)

Our work establishes the importance of the Fab-7 boundary element for insulation between 3D domains marked by different histone modifications. (biorxiv.org)

In addition to its roles in the regulation of inflammatory diseases and the maintenance of local immune cells, CCL5 expressed by cancer cells plays diverse roles in shaping cancer microenvironments toward their own survival. (nature.com)
The purpose of this study was to clarify the roles of paraspeckles in PXR-mediated transcriptional regulation. (aspetjournals.org)

By whole transcriptome analyses, we characterized glucosedependent and CcpA-dependent gene regulation in Clostridium difficile. (studyres.com)

Transcription factors (TFs) bind to specific regions of DNA known as transcription factor binding sites (TFBSs) and modulate gene expression by interacting with the transcriptional machinery. (bvsalud.org)
The binding of TFs to TFBSs influences the recruitment of the transcriptional machinery, thereby regulating gene transcription in a precise and specific manner. (bvsalud.org)

We used a large number of existing chromosomal aberrations in the Scr homeotic gene to investigate the functions of the cis -acting elements within the endogenous gene. (nih.gov)

Thus, the first events of embryonic development along the D/V axis are controlled at the transcriptional level. (silverchair.com)

Gene structure is the organisation of specialised sequence elements within a gene. (wikipedia.org)
Each of these steps is controlled by specific sequence elements, or regions, within the gene. (wikipedia.org)
Every gene, therefore, requires multiple sequence elements to be functional. (wikipedia.org)
The organization, sequence and transcriptional regulation of the murine S100{dollar}\beta{dollar} gene have been studied. (umaryland.edu)
You should be able to draw /identify/annotate when given a gene sequence the elements of the gene above. (pressbooks.pub)
The L1 genomic sequence is ~ 6 kb in length and contains two open reading frames (ORF1 and ORF2), with molecular masses of ~ 40 and 150 kDa, respectively. (researchsquare.com)

negative regulation. (online-sciences.com)
This finding provides a novel concept that paraspeckles formed by liquid-liquid phase separation potentially affect drug metabolism via negative regulation of PXR function. (aspetjournals.org)

These common elements largely result from the shared ancestry of cellular life in organisms over 2 billion years ago. (wikipedia.org)
12/95-5/2000 Associate Professor, Departments of Medicine and Cellular and Molecular Medicine, University of Ottawa. (vdocuments.net)

Truncated NPAS3 lacking transactivation domain was also found to activate VGF, which suggested that not only transactivation domain but other structural motifs were also involved in the regulation. (frontiersin.org)
We discuss how co-operativity allows simple static motifs to perform the conditional regulation that underlies decision-making in higher eukaryotic biological systems. (biomedcentral.com)

Cloning and molecular characterization of the murine S100(beta), a glial-specific neurotrophic factor in the nervous system. (umaryland.edu)

The results suggest that their interactions are involved in renin transcriptional regulation. (biomedcentral.com)

The major events in the regulation of the host response on a transcriptional level occur within the first 3 days after infection. (biomedcentral.com)
A luciferase assay using a plasmid containing the PXR response elements of CYP3A4 revealed that the increased CYP3A4 induction by siNEAT1_2 or siDAZAP1 was due to the increased transactivation by PXR. (aspetjournals.org)
It was determined that Ear2 negatively regulates renin expression by competing with retinoic acid receptor/ retinoid X receptor (RAR/RXR) for binding to the retinoic acid response elements (RARE) on the renin enhancer [ 13 ]. (biomedcentral.com)

Previously, we have demonstrated that NPAS3 regulates VGF at the transcriptional level. (frontiersin.org)

This factor can specifically bind to a 17 bp DNA fragment ({dollar}-{dollar}1513/{dollar}-{dollar}1497) which contains a consensus silencer element. (umaryland.edu)

Molecular cell. (rochester.edu)
Cell adhesion molecules: implications for a molecular histology. (academicinfluence.com)
Cell adhesion molecules in the regulation of animal form and tissue pattern. (academicinfluence.com)
The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain. (hindawi.com)

This is required for nuclear translocation and transcriptional activity of NF-jB. (lsd1receptor.com)

Anatomy16

Organisms10

Diseases1

Chemicals and Drugs88

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes99

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Post-transcriptional6

Drosophila5

Enhancers4

Sequences7

Gene Expression1

Regulators2

Repressor2

Genetics2

Regulatory19

MRNA1

Promoters1

Genes7

Promoter6

Proteins1

Biology3

Protein5

Regulator2

Mechanisms2

Repression1

Chromatin structure1

Silencing2

Methylation1

Activator1

Epigenetic regulation4

Processes2

Targets2

Basal2

Histone1

Roles2

Analyses1

Machinery2

Endogenous1

Embryonic development1

Sequence6

Negative regulation2

Cellular2

Motifs2

Characterization1

Interactions1

Response3

Level1

Factor1

Cell4

Nuclear1