AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesInformation ScienceHealth Care
Behavior, AnimalSubstrate SpecificityMolecular Sequence DataAmino Acid SequenceKineticsSensitivity and SpecificityBinding SitesModels, MolecularCatalysisSequence Homology, Amino AcidCatalytic DomainEscherichia coliRecombinant ProteinsHydrolysisBase SequenceCrystallography, X-RayMutagenesis, Site-DirectedHydrogen-Ion ConcentrationCloning, MolecularProtein BindingProtein Structure, TertiarySequence AlignmentProtein ConformationStructure-Activity RelationshipBacterial ProteinsMutationSpecies SpecificityAntibody SpecificityIsoenzymesMolecular WeightElectrophoresis, Polyacrylamide GelMolecular StructureAmino AcidsPeptidesEnzyme StabilityPhosphorylationAmino Acid SubstitutionOligopeptidesStereoisomerismTemperatureRecombinant Fusion ProteinsCell LineDNA PrimersEndopeptidasesSerine EndopeptidasesChromatography, High Pressure LiquidAlcohol OxidoreductasesProtein Structure, SecondaryCarbohydrate SequenceSaccharomyces cerevisiaeEscherichia coli ProteinsCattleProtein EngineeringPhylogenyEnzyme ActivationOxidation-ReductionModels, ChemicalAcyltransferasesChromatography, GelAmino Acid MotifsDNA, ComplementaryPeptide LibraryGlycoside HydrolasesDNAOligosaccharidesLiverBiological TransportOxidoreductasesHydrolasesCarrier ProteinsAdenosine TriphosphatePlasmidsAcyl Coenzyme ABiocatalysisMembrane Transport ProteinsPeptide HydrolasesProtein KinasesConserved SequenceAminopeptidasesbeta-GlucosidasePseudomonasMixed Function OxygenasesCarboxylic Ester HydrolasesMutagenesisPeptide FragmentsEnzyme InhibitorsDioxygenasesCations, DivalentCysteine EndopeptidasesMembrane ProteinsProtease InhibitorsAmidohydrolasesRabbitsBinding, CompetitiveModels, BiologicalSaccharomyces cerevisiae ProteinsAlanineMass SpectrometryEsterasesStreptomyces
BiologyBroad substrate specificityPhosphorylationResiduesProteins2021KinaseDeterminantsAntifungal AgentsDifferentiallyMechanismCharacterizationEnzymesCatalyticPeptidesMonomericBasisPharmacologyDynamics simulationVivoProteinPseudomonasColloidalBiologicalCellHumanInteractionActivityStudyBiotechnologyJournalInsightsModelFoundDomains
Biology8
  • Appreciating the similarities and differences between cell types is particularly important to the fields of cell and molecular biology as well as to biomedical fields such as cancer research and developmental biology. (phys.org)
  • Hence, research in cell biology is closely related to genetics, biochemistry, molecular biology and developmental biology. (phys.org)
  • In 1984, he joined the staff of the Medical Research Council Laboratory for Molecular Biology (MRC-LMB) in Cambridge, where he started working on how transcription is regulated by growth factors. (crick.ac.uk)
  • Journal of Molecular Biology , 392 , 1266-1277. (elsevierpure.com)
  • The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA and/or RNA preparations from microorganisms, cells, and plants. (zymoresearch.com)
  • The subsequent chapters describe the structure, assembly, disassembly, and molecular biology of the Chlamydomonas reinhardtii cell wall. (elsevier.com)
  • p. 55-77 (Methods in molecular biology (Clifton, N.J. (lu.se)
  • So-called lock-and-key interactions, i. e., the preferred association of molecular building blocks with complementary shapes, are omnipresent in biology, where they for example describe the interactions between enzymes and their substrates. (lu.se)
Broad substrate specificity2
Phosphorylation7
  • Molecular modeling and dynamics simulation indicated that the phosphorylation site of the peptide may have to adopt a turn-like conformation that will orientate the charged and hydrophobic residues so as to allow interaction with postulated binding surfaces within the CDCS active site. (unityfvg.it)
  • Here we compare the specificity of two budding yeast cyclins, the S-phase cyclin Clb5 and the M-phase cyclin Clb2, in the phosphorylation of 150 Cdk1 (Cdc28) substrates. (nature.com)
  • Phosphorylation of Clb5-specific targets during S phase was reduced by replacing Clb5 with Clb2 or by mutating the substrate RXL motif, confirming the importance of Clb5 specificity in vivo . (nature.com)
  • Although we did not identify any highly Clb2-specific substrates, we found that Clb2-Cdk1 possessed higher intrinsic kinase activity than Clb5-Cdk1, enabling efficient phosphorylation of a broad range of mitotic Cdk1 targets. (nature.com)
  • Thus, Clb5 and Clb2 use distinct mechanisms to enhance the phosphorylation of S-phase and M-phase substrates. (nature.com)
  • Figure 3: Clb5-specific substrate phosphorylation occurs in vivo . (nature.com)
  • Structure of substrate-bound SMG1-8-9 kinase complex reveals molecular basis for phosphorylation specificity. (mpg.de)
Residues5
  • To investigate the molecular basis for this selectivity, we identified a region of SULT1A3, which, we hypothesized, contributes to its preference for biogenic amines, and mutated two amino acids within this domain to the corresponding residues in a closely related but functionally distinct phenol sulfotransferase, SULT1A1 (H143Y and E146A). (aspetjournals.org)
  • Peroxidation products were identified in three relatively minor classes of anionic phospholipids, cardiolipin, phosphatidylserine, and phosphatidylinositol, whereby oxygenation of polyunsaturated fatty acid residues also showed unusual substrate specificity. (cdc.gov)
  • The ternary complex of ST8SiaIII with a donor sugar analog and a sulfated glycan acceptor identified with a sialyltransferase glycan array provides insight into the residues involved in substrate binding, specificity and sialyl transfer. (rcsb.org)
  • Based on a predicted model of UMAMIT29, we propose that the substrate transporting cavity consists of 51 residues, of which four are highly conserved residues across all the analyzed homologs of UMAMIT29. (frontiersin.org)
  • Based on our model, we proposed a substrate-transporting cavity of UMAMIT29 defined by helix I to IV and VI-IX and selected 51 residues as putative substrate binding sites based on solvent accessibility ( Figure 1 ). (frontiersin.org)
Proteins8
  • The P-loop is best known for its presence in ATP- and GTP-binding proteins, and is also found in a variety of proteins with phosphorylated substrates. (wikipedia.org)
  • In addition, the molecular function of some of the described proteins suggests a central role for SVEVs in the cytotoxicity of the snake venom and sheds new light in the envenomation process. (nature.com)
  • Certain very abundant ribosomal proteins can be the sacrificial substrates targeted for degradation at the onset of the downshift. (go.jp)
  • Several molecular mechanisms with antifungal agents have been reported for C. albicans where insertions, deletions, and point mutations in genes codifying target proteins are frequently related to the antifungal drug resistance. (intechopen.com)
  • This led to molecular characterisation of the TCF family of SRF cofactors as targets for MAP kinase signalling, and the demonstration that the MRTF transcription cofactors are novel G-actin binding proteins that sense fluctuations in G-actin concentration. (crick.ac.uk)
  • The substrate specificities and affinities of substrate binding proteins MalE1 and MalE2 were measured and found to differ and are also expressed under different growth conditions. (uconn.edu)
  • Six other putative sugar binding proteins from examined and four of them were found to be binding to ribose, xylose, myo-inositol and digalacturonic acid with high affinities and specificities. (uconn.edu)
  • Although the overall sensitivity and specificity of the Western blot for detection of antibodies to the various viral proteins are high, there has been substantial debate regarding the interpretive criteria. (cdc.gov)
20211
  • 2021 ). At present, based on the pectin molecular domain-connection method, it is believed that the galacturonic acid-containing unit is the "smooth region," while the part containing rhamnogalacturonan I and II is called the "hairy region" (Adetunji et al. (springeropen.com)
Kinase4
  • A systematic study was undertaken in order to assess the substrate specificity of cyclin-B/cell division control protein kinase (CDC2) isolated from human HeLa cells, using 13-15 residue peptides with a central histone-like KKSPKK motif as a model. (unityfvg.it)
  • Identification of a cyclin-cdk2 recognition motif present in substrates and p21-like cyclin-dependent kinase inhibitors. (nature.com)
  • Schulman, B. A., Lindstrom, D. L. & Harlow, E. Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A. (nature.com)
  • This is particularly important for LIMK2, a kinase almost three decades old, as only a handful of its substrates are known to date. (bvsalud.org)
Determinants2
  • Dr. Mundorff's research interests are in the investigation of determinants of enzyme substrate specificity, using both study of mutants and computer modeling using specialized molecular modeling software. (hofstra.edu)
  • However, both the substrate transporting cavity and the determinants of substrate specificity within the cavity of UMAMITs for any substrate remains unknown. (frontiersin.org)
Antifungal Agents1
  • This chapter summarizes the main molecular mechanisms to C. albicans antifungal drug resistance, besides offering an overview of new antifungal agents and new antifungal targets to combat fungal infections. (intechopen.com)
Differentially2
  • A- and B-type cyclins differentially modulate substrate specificity of cyclin-cdk complexes. (nature.com)
  • A comparison of the putative substrate binding site of homologs within the brassicaceous-specific, glucosinolate-transporting clade with the non-brassicaceous-specific, non-glucosinolate-transporting UMAMIT32 clade identified 11 differentially conserved sites. (frontiersin.org)
Mechanism4
  • Journal Article] Molecular mechanism of strict substrate specificity of an Extradiol Dioxygenase, DesB, derived from Sphingobium sp. (nii.ac.jp)
  • Based on substrates and mechanism of action, pectinase is classified into the following sub-types: protopectinase, pectinesterase, and depolymerase (Kashyap et al. (springeropen.com)
  • By using microsecond standard and accelerated molecular dynamics simulations, we provide for the first time a detailed molecular mechanism of allosteric regulation of RNMT by RAM. (dundee.ac.uk)
  • This review focuses on LIMK2's unique catalytic mechanism, substrate specificity and its upstream regulators at transcriptional, post-transcriptional and post-translational stages. (bvsalud.org)
Characterization1
Enzymes2
  • The enzymes were extensively characterized for activity, specificity, and inhibition using a UV spectrophotometric assay. (rhea-db.org)
  • 6]. Some of the best examples of high-molecular-weight sectors, workers are at increased risk of becoming sensitized occupational allergens are the fungal enzymes. (cdc.gov)
Catalytic1
  • The change of a single amino acid, E146A, was sufficient to transform the catalytic properties and substrate preference of SULT1A3, such that they closely resembled those of SULT1A1. (aspetjournals.org)
Peptides1
  • Brown, N. R., Noble, M. E., Endicott, J. A. & Johnson, L. N. The structural basis for specificity of substrate and recruitment peptides for cyclin-dependent kinases. (nature.com)
Monomeric2
  • 2015). The peroxisomal protein import machinery displays a preference for monomeric substrates . (up.pt)
  • Within the cleft, at least five different subsites interact with the substrate, each subsite binding one monomeric unit of the polysaccharide. (lu.se)
Basis1
  • They are also providing the basis for the formation of well-defined molecular complexes using supramolecular chemistry. (lu.se)
Pharmacology2
  • Thank you for sharing this Molecular Pharmacology article. (aspetjournals.org)
  • Message Body (Your Name) thought you would be interested in this article in Molecular Pharmacology. (aspetjournals.org)
Dynamics simulation1
  • After approximately 20 ns of molecular dynamics simulation, the structure of the pectin lyase PGLA-rep4 tended to be stable. (springeropen.com)
Vivo1
  • Thus, in vitro analysis of substrate spectra using recombinant transferase domains corresponding to the auto-proteolytically cleaved domains, predicts more precisely the in vivo substrates than the full length toxins. (pasteur.fr)
Protein3
  • Substrate-specificity of the DNA-protein crosslink repair protease SPRTN. (uni-muenchen.de)
  • At the molecular level, it was found that replacement of glucose by starch has enhanced the relative expression level of ccpA encoding catabolite control protein A. Therefore, the repression of starch utilization by glucose could be probably relieved. (hindawi.com)
  • Based on distance-based protein folding tools, we predict the substrate-transporting cavity of UMAMIT29. (frontiersin.org)
Pseudomonas1
  • Molecular epidemiology of Pseudomonas aeruginosa bloodstream infection isolates in a non-outbreak setting. (cdc.gov)
Colloidal2
  • This approach from the molecular world has now been successfully transferred to the colloidal domain, i.e. to much larger synthetic particles with complementary shapes. (lu.se)
  • Supported by model calculations, the experiments demonstrate the high specificity of the assembly highlighted by the formation of defined clusters, so-called colloidal molecules. (lu.se)
Biological1
  • Consequently, small-molecule drug discovery usually involves an iterative process of molecular design, chemical synthesis, biological assay and data analysis feeding directly into the next cycle, but this process always needs a chemical starting point. (ddw-online.com)
Cell6
  • Proteomic exploration of molecular mechanisms of HIV-1 infection in the host cell. (uni-muenchen.de)
  • Biochimica et Biophysica Acta - Molecular Cell Research, 1863 (1), 139 - 147. (up.pt)
  • Biochimica et Biophysica Acta - Molecular Cell Research, 1823 (10), 1958 - 1966. (up.pt)
  • Biochimica et Biophysica Acta - Molecular Cell Research, 1793 (11), 1669 - 1675. (up.pt)
  • Biochimica et Biophysica Acta - Molecular Cell Research, 1773 (7), 1141 - 1148. (up.pt)
  • We used the engineered cell library to dissect the binding specificity of galectin-4 and confirmed binding to the 3-O-sulfo-core1 O-glycan. (lu.se)
Human3
  • 1(2-Acetoxyethyl)quinuclidinium iodide has been examined as a substrate of both purified acetylcholinesterase and human serum cholinesterase. (aspetjournals.org)
  • As part of this function, human K1 exhibits unique dual-substrate specificity in hydrolyzing low molecular weight kininogen between both Arg-Ser and Met-Lys sequences. (rcsb.org)
  • The solvent networks within the S1 and S1' pockets suggest how the Arg-Ser and Met-Lys dual substrate specificity of human K1 is accommodated. (rcsb.org)
Interaction3
  • Clb5 specificity depended on an interaction between a hydrophobic patch in Clb5 and a short sequence in the substrate (the RXL or Cy motif). (nature.com)
  • Figure 2: Clb5 specificity depends on an interaction between the Clb5 hydrophobic patch and an RXL motif in the substrate. (nature.com)
  • We are interested in the substrate - enzyme interaction, and especially in the interactions that are likely to direct the specificity of the enzyme. (lu.se)
Activity4
  • Since avian influenza receptor-binding specificity and NA sialidase activity. (cdc.gov)
  • The enzyme activity of PGLA-rep4, measured at 235 nm with 0.2% apple pectin as the substrate, was 554.0 U/mL, and the specific enzyme activity after purification using a nickel column was 822.9 U/mg. (springeropen.com)
  • Analysis of substrate analogs revealed that specific activity rapidly dropped as the length of the 5'-alkylthio substitution increased. (rhea-db.org)
  • However, the molecular mechanisms leading to its upregulation and its deregulated activity in various diseases largely remain unknown. (bvsalud.org)
Study1
  • This study evaluated the specificity of the YTHDC2/LIMK1/eIF2α signalling axis and the efficacy of related drugs in modulating 5-FU sensitivity in CRC. (bvsalud.org)
Biotechnology1
  • Molecular Biotechnology. (up.pt)
Journal1
  • I. Theory and applications to the system acetylcholinesterase-substrate Journal of the American Chemical Society . (academictree.org)
Insights1
  • Important insights into approaches to identify critical molecular domains and the complexity of relating defined molecular associations to establishment of matrix architecture are provided. (elsevier.com)
Model1
  • 1955 ) A simple model of molecular specificity in enzyme-substrate systems. (academictree.org)
Found1
  • No oxidized molecular species were found in the two most abundant phospholipid classes: phosphatidylcholine and phosphatidylethanolamine. (cdc.gov)
Domains1
  • The substrate profile of the toxins was investigated with recombinant LCGT transferase domains (rN) and a wide panel of small GTPases. (pasteur.fr)