• A key point of the reference genome is that it is not a single sequence. (scienceblogs.com)
  • Humans have been mapping the world for several millennia, but genomes have been mapped and explored for just a single century with the greatest advancements in making a sequence reference map of the human genome possible in the past 30 years. (scienceblogs.com)
  • After the deoxyribonucleic acid (DNA) sequence of the human genome was completed in 2003, the reference sequence underwent several improvements and today provides the underlying comparative resource for a multitude genetic assays and biochemical measurements. (scienceblogs.com)
  • A reference genome sequence is a map that provides the essential coordinate system for annotating the functional regions of the genome and comparing differences between individuals' genomes. (scienceblogs.com)
  • The reference genome sequence is always product of understanding at a set point in time and continues to evolve. (scienceblogs.com)
  • The current reference genome sequence is a collection of sequences, an assembly, that include sequences assembled into chromosomes, sequences that are part of structurally complex regions that cannot be assembled, patches (fixes) that cannot be included in the primary sequence, and high variability sequences that are organised into alternate loci. (scienceblogs.com)
  • The effort is part of the DOD SARS-CoV-2 Whole Genome Sequencing Action Plan, which brings together laboratories to conduct whole genome sequencing across the Military Health System in response to the COVID-19 pandemic. (health.mil)
  • The staff at Naval Health Research Center (NHRC) added whole genome sequencing capability to their surveillance program. (health.mil)
  • That might be sending a particularly large number of genome sequences from the UK to Finland, for example. (newscientist.com)
  • Genome Sequencing for Healthy Individuals? (cdc.gov)
  • To study the genomic divergences among hominoids and to estimate the effective population size of the common ancestor of human and chimpanzee we selected 53 autosomal intergenic noncoding DNA segments from the human genome and sequenced them in a human, chimpanzee, gorilla, and orangutan. (ncl.edu.tw)
  • Microbiological, quantitative gene expression, mRNA half-life and genome data for 11 strains of S. aureus combined with bioinformatic analyses were used to identify correlates of increased MDR EP gene expression. (manchester.ac.uk)
  • A Custom Hepatitis A Virus Assay for Whole-Genome Sequencing. (cdc.gov)
  • Tracing Foodborne Botulism Events Caused by Clostridium botulinum in Xinjiang Province, China, Using a Core Genome Sequence Typing Scheme. (cdc.gov)
  • Isolates are characterized using whole genome sequencing to determine serogroup and molecular typing information. (medscape.com)
  • Thanks to the advancement of sequencing technology, the plastid genome and the whole genome were sequenced in some durian cultivars. (who.int)
  • This was confirmed by the high similarity (99.2%-100%) of their sequences with those available in GenBank. (who.int)
  • These estimates, which were confirmed by additional data from GenBank, are substantially lower than previous ones, which included repetitive sequences and might have been based on less accurate sequence data. (ncl.edu.tw)
  • Molecular Phylogenetics and Evolution , 40(2): 317-331. (fieldmuseum.org)
  • Phylogenetics, the use of molecular sequencing data, such as DNA, to trace historical relationships among people and chart the transmission of viruses. (hhrjournal.org)
  • They are widely used for the development of in-proprietary databases of protein, nucleic acid, and other biomolecule sequences. (marketresearch.com)
  • Major technological advances in affordable nucleic acid sequencing have allowed for an explosion of sequencing data and molecular tools available for researchers in biological sciences. (lu.se)
  • Sequencing was performed with both the forward and reverse primers. (uni-goettingen.de)
  • ABSTRACT A diagnostic polymerase chain reaction (PCR) assay using species-specific primers and direct sequencing was used to identify members of the Anopheles maculipennis complex in the north-west and central regions of the Islamic Republic of Iran. (who.int)
  • QIAseq Targeted RNA Indices are for indexing samples for targeted RNA sequencing and primers necessary for sequencing RNA libraries generated by QIAseq Targeted RNA Panels. (qiagen.com)
  • 5-8 Lesions have been historically associated with Chlamydiales species and pan-chlamydial primers have been successfully used to obtain sequence data for a spotted eagle ray and a leopard shark. (vin.com)
  • Initial PCR with pan- Chlamydiales 16S rRNA gene primers and resultant molecular sequence data support findings that epitheliocystis could be caused by unclassified proteobacteria species. (vin.com)
  • We compared the sequences of two housekeeping genes ( hspA and glmM ) from these clones with those of 131 unrelated strains from patients living in different geographic regions. (cdc.gov)
  • Clonal descent has been demonstrated by comparing alleles of genes such as vacA , flaA , and flaB of isolates infecting members of the same family ( 10 - 12 ) and by sequencing three housekeeping genes ( ureI , atpA , and ahpC ) ( 13 ). (cdc.gov)
  • Analysis of the sequences of housekeeping genes ( atpD , scoB , glnA and recA ) showed that strains cluster according to their geographic origins ( 15 , 20 ). (cdc.gov)
  • To assess the genetic diversity and relationships between the isolates, we sequenced two housekeeping genes ( glmM and hspA ). (cdc.gov)
  • A subfamily-level study of the Veneridae was carried out, based on DNA sequence data of three genes (16S, COI and H3). (fieldmuseum.org)
  • Molecular sequence data of three genes were analyzed together with morphological characters and resulted in a new classification for the subfamily. (fieldmuseum.org)
  • With the rising application of informatics solutions, gene sequencing is done through the identification of proteins and genes, which could further help in the development of potential drugs. (marketresearch.com)
  • you will see in a live demonstration how to use the Entrez interface including filters, the advanced search page, and search strategies to collect and download a specific set of records, to narrow the search, and to use the precomputed relationships available in the Entrez system find related sequences, genomic regions, genomic maps, homologous genes and proteins, pathways and expression information. (piug.org)
  • In addition to the sequence databases, you will also learn how to access related information in HomoloGene, UniGene and Gene Expression Omnibus (GEO), how to use precomputed BLAST (BLink) to find homologous proteins and genes, and how to use the Graphical Sequence Viewer as a tool for exploring large and complex genomic sequences and their annotations. (piug.org)
  • Innovative UMIs (unique molecular indices) eliminate PCR duplication and amplification bias to deliver the most accurate results (see figure Unbiased and accurate gene quantification (A) Different amounts (20 ng, 5 ng or 1.25 ng) of universal reference RNA were used to determine expression levels of three genes (BNIP1, CTNND2 and DAPK1) using targeted RNAseq. QIAseq digital RNA sequencing method (A; molecular barcode counts) showed accurate quantification of all three genes corresponding to different RNA input, whereas traditional targeted RNAseq (B; read counts) revealed PCR duplication limitation and yielded inaccurate quantification. "> Unbiased and accurate gene quantification ). (qiagen.com)
  • The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)
  • The data revealed reliable data on the structure and function of several genes. (who.int)
  • Around the world, geneticists and molecular biologists are busy sequencing proteins and genomes. (newscientist.com)
  • In a 2013 blog post, we asked the question: "When should we all have our genomes sequenced? (cdc.gov)
  • The data were collected from capillary electrophoresis on the ABI Prism 3100® Genetic Analyzer with the Sequence Analysis Software v3.1 (Applied Biosystems). (uni-goettingen.de)
  • This web-based application performed pairwise comparisons of HIV-1 protease and partial reverse transcriptase to measure sequence relatedness and identify sequences that were highly genetically similar at a genetic distance of ≤0.015 substitutions per site ( 6 , 7 ). (cdc.gov)
  • We report on the incidence, genetic diversity, and molecular epidemiology of sapoviruses detected in domestic pigs in a comprehensive study conducted in six European countries (Denmark, Finland, Hungary, Italy, Slovenia, and Spain) between 2004 and 2007. (lu.se)
  • Genetic analysis is error prone and the data require validation because the methods for collecting DNA sequences create artifacts and the reference sequence used for comparative analyses is incomplete. (scienceblogs.com)
  • It is becoming increasingly quick, cheap and easy for scientists to collect vast amounts of data, whether it's about people's shopping habits, internet usage, or even their genetic make-up. (newscientist.com)
  • Over the past five years or so, much of the genetic data sent to the EBI was on viruses, bacteria and animals. (newscientist.com)
  • The project's goals include using the genetic data to help diagnose and treat illnesses such as cancer within the next four years. (newscientist.com)
  • Olivo Miotto and his colleagues at the Centre for Genomics and Global Health are collecting and mining another set of genetic data for clues about the spread of drug-resistant malaria. (newscientist.com)
  • We have brought together a network of collaborations to create a global resource of genomic data on genetic variation of the Plasmodium falciparum parasite which causes malaria," he says. (newscientist.com)
  • CONCLUSIONS: Despite the presence of sequence variations in their respective genetic backgrounds, within each line the sequence of crx was identical. (zfin.org)
  • Cross-referencing with USGENE is possible, with direct links from the well-known genetic sequence database to corresponding PatBase Express records. (piug.org)
  • Therefore, molecular biology is a powerful tool to approach and explore the genetic characteristics of durians. (who.int)
  • The molecular data and relative distribution of these species in relation to their vectorial capacity and the epidemiology of malaria in the region are discussed. (who.int)
  • Epidemiology and Molecular Characteristics of mcr-9 in Citrobacter spp. (cdc.gov)
  • Molecular phylogeny of tribe Dipterocarpeae (family Dipterocarpaceae) based on sequence data of chloroplast and nuclear DNA. (uni-goettingen.de)
  • 2006. Molecular phylogeny of Dipterocarpaceae in Indonesia based on chloroplast DNA. (uni-goettingen.de)
  • Before a tree, or phylogeny, can be determined, DNA and protein sequence data must be organized. (sciencedaily.com)
  • 2017). Xibalbanus cozumelensis, a new species of Remipedia (Crustacea) from Cozumel, Mexico, and a molecular phylogeny of Xibalbanus on the Yucatán Peninsula. (uib.no)
  • 2006. Phylogeny of venus clams (Bivalvia: Venerinae) as inferred from nuclear and mitochondrial gene sequences. (fieldmuseum.org)
  • Molecular phylogeny and divergence times of deuterostome animals. (palass.org)
  • Critical Reviews in Biochemistry and Molecular Biology. (cdc.gov)
  • I pointed out the illegitimacy of some of the molecular comparisons that ignore intergenic regions in Lungfish and humans -- famous novel has almost 100% similarity to mirriam websters dictionary . (uncommondescent.com)
  • The clusters were detected through routine analysis of HIV-1 nucleotide sequence data obtained through public health surveillance ( 1 , 2 ). (cdc.gov)
  • In February 2021, GDPH identified three HIV clusters among Hispanic MSM using molecular analysis of HIV-1 nucleotide sequence data collected through routine surveillance ( 1 ). (cdc.gov)
  • Highly repetitive sequences present in non-coding regions of crx and extended tetra-nucleotide repeats in intronic regions were associated with sequence variation between different strains. (zfin.org)
  • You will understand the scope and content of the sequence databases (Nucleotide, GSS, EST, Protein and SRA). (piug.org)
  • This workshop will explain how to search sequences with uncommon amino acid residues, sequences that have been modified, and sequences that use variables for amino acid or nucleotide residues. (piug.org)
  • This review aims to link recent molecular data, often translated into amino acid sequences and predicted three dimensional structural motifs, to known mechanical properties. (bioone.org)
  • WGS offers a more precise, close-up look at these diseases and allows doctors and scientists to monitor disease evolution at a molecular level. (health.mil)
  • This pioneer research has significant impacts on future studies in molecular evolution. (ncl.edu.tw)
  • Molecular Biology and Evolution , 22 , 2275-2284. (palass.org)
  • Bio++: efficient, extensible libraries and tools for computational molecular evolution. (who.int)
  • In particular, the dataset available from the Irvine repository was drawn from a compilation of promoter sequences that were preprocessed to conform to the biologists' related notion of the consensus sequence, a first-order approximation with a number of shortcomings that are well-known in molecular biology. (aaai.org)
  • This provides the opportunity for ecologists, evolutionary biologists and molecular biologists to incorporate bioinformatic analyses into their existing research program to approach their research questions from an interdisciplinary angle. (lu.se)
  • DNA sequences evolve through duplication and mutation and, as a result, contain many repeated sequences of different sizes, which complicates data analysis. (scienceblogs.com)
  • Areas that will be covered include: sequence databases, pairwise and multiple sequence alignment, homology searches in sequence databases and subcellular localization prediction. (lu.se)
  • The science of figuring out these trees, known as systematics, has progressed significantly in the last two decades largely due to advances in computation, genetics and molecular biology. (sciencedaily.com)
  • 2) test the utility of these sequences for phylogenetic construction of interspecific relationships. (uni-goettingen.de)
  • For multiple alignment, the BioEdit Sequence Alignment Editor version 7.0.5.3 (Hall 1999) was used and the phylogenetic analysis was carried out with PAUP* v4.0b10 (Swofford 1998). (uni-goettingen.de)
  • Strain relationships within the family were determined by analyzing allelic variation at both loci and building phylogenetic trees and by using multilocus sequence typing. (cdc.gov)
  • The problem is this is a phylogenetic reconstruction, not one based purely on characters or a fair view of molecular data. (uncommondescent.com)
  • Amplicons were sequenced and sequence data assembled into contigs. (zfin.org)
  • The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees high specificity and accurate results (see figure Proprietary primer design delivers gene-specific amplicons (>97% specificity) Sequencing libraries were prepared using 1.25, 5 or 20 ng universal reference RNA and QIAseq Targeted RNA Panels, ranging from 12-plex to 1000-plex. Sequencing was performed on the Illumina MiSeq, dedicating 1 million reads per sample. Specificity is calculated as percent of trimmed and mapped reads that map to intended targets. "> Proprietary primer design delivers gene-specific amplicons - 97% specificity ). (qiagen.com)
  • Specimens were collected from 9 provinces during 2 seasonal activities in 2001-2002, identified morphologically and subjected to PCR assay and direct sequencing. (who.int)
  • NHRC continues to provide critical sequencing and epidemiological support for the COVID-19 efforts and have even developed a new serological quantitative assay that enables the differentiation between the immune response generated by natural infection compared to immunity generated through vaccination. (health.mil)
  • In fact, UMIs (unique molecular indices) entirely remove PCR bias in RNAseq counting (see figure Unmatched uniformity (>97% of assays are within 20% of median molecular tag counts) A 917-plex gene panel was used to prepare a library from 10 ng of NA12878 reference DNA. All assays were designed to be intra-exon, and are thus single copy on genomic DNA. This allows an estimation of the uniformity of amplicon performance in the library preparation step (e.g., every unique tag equals one captured copy). In terms of raw assay performance, 97.5% of assays are within 20% of mean/median molecular tag counts. For cataloged panels, any assay below 20% is redesigned and replaced. Molecular barcodes entirely remove this variation in RNAseq counting. "> Unmatched uniformity - 97% of assays are within 20% of median molecular tag counts ). (qiagen.com)
  • In 2021, molecular HIV analysis in Georgia identified clusters of rapid HIV transmission among Hispanic or Latino (Hispanic) gay, bisexual, and other men who have sex with men (MSM) in metropolitan Atlanta. (cdc.gov)
  • Most molecular linkages in Wisconsin were among persons within the same racial/ethnic, risk, and age groups. (cdc.gov)
  • Among named partner linkages where both persons had an HIV sequence available, 33.8% also had a molecular linkage and were deemed plausible transmission partners. (cdc.gov)
  • The Wisconsin Division of Public Health (DPH) analyzed HIV sequence data generated from provider-ordered drug resistance testing and collected through routine HIV surveillance to identify molecular clusters and describe demographic and transmission risk characteristics among pairs of persons whose sequences were highly genetically similar (i.e., molecular linkages). (cdc.gov)
  • In addition, overlap between partner linkages identified during public health interviews and molecular linkages was assessed. (cdc.gov)
  • Among named partner linkages identified during interviews in which both persons also had a reported sequence, overlap of named partner and molecular linkages was moderate: 33.8% of named partners were plausible transmission partners according to available molecular data. (cdc.gov)
  • You will also explore aspects of the literature databases (PubMed and PubMed Central) with emphasis on the linkages between the literature and molecular data. (piug.org)
  • H. pylori is one of the most genetically diverse bacterial species, displaying from 2.7% to 8.0% of DNA sequence polymorphism ( 14 - 16 ). (cdc.gov)
  • It will enable the creation of much more accurate trees, especially for the Tree of Life, which deals with hundreds of thousands of gene sequences from the millions of species on Earth. (sciencedaily.com)
  • Members of several species pairs that are morphologically very similar actually belong to different subfamilies according to molecular data and, because these groups are not basal, similar morphological characters apparently evolved in parallel. (fieldmuseum.org)
  • All 18 Recent species were redescribed and information on distribution, synonymy, taxonomy, and newly generated anatomical data were compiled. (fieldmuseum.org)
  • Biogeographic reconstruction based on a resequenced data set of 26 species suggested that Syringa originated in the northern part of East Asia during the Miocene (approximately 14.73 Mya) and that the five Syringa groups initially formed before the Late Miocene (approximately 9.97 Mya). (bvsalud.org)
  • Tetrapetide sequences and structures were expressed by letter codes. (medscimonit.com)
  • RESULTS: High values of the rho-coefficient extracted sequences of strong structural determinability and structures of high sequence selectivity. (medscimonit.com)
  • Utopia Documents also connects literature to underlying public datasets, provides ways to export and graph tabular data, enables manipulation of molecular structures and sequence data, and changes references into hyperlinks. (neb.com)
  • New England Biolabs is supporting improvements to the interactivity of scientific literature by providing data about our library of molecular biology reagents and their applications. (neb.com)
  • NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, cellular analysis, epigenetics and RNA analysis. (neb.com)
  • Microbiological and gene expression data correlated in four instances, existing knowledge of the substrate specificity of NorC resulted in correlation in two others, and a transcriptional/translational disconnect is possible for the remaining two. (manchester.ac.uk)
  • Analysis of HIV sequence data is a useful tool for characterizing transmission patterns not immediately apparent using traditional public health interview data, even in a state with lower HIV morbidity. (cdc.gov)
  • Jan. 31: Character analysis -- what is a data matrix? (berkeley.edu)
  • During the COVID-19 pandemic, NHRC brought on scientists and lab technicians to support this work and bioinformatics, which enriched their data collection and analysis capabilities. (health.mil)
  • This data was handed off to NHRC scientists for process and analysis," said U.S. Navy Lt. Cmdr. (health.mil)
  • In 2020, the sequence analysis market segment accounted for the largest share and is likely to register a significant market growth over the study period. (marketresearch.com)
  • For sequence analysis, bioinformatics tools play an important role. (marketresearch.com)
  • Basic drug discovery requires the involvement of different sets of databases, sequence analysis tools, such as BLAST, FASTA, and CLUSTALW. (marketresearch.com)
  • Get data-driven expert analysis from the CB Insights Intelligence Unit. (cbinsights.com)
  • 6,8,9 Recently, advanced diagnostic techniques and molecular analysis have suggested the lesions are actually associated with proteobacteria. (vin.com)
  • Supplementing named partner data with molecular data might detect HIV transmission networks not elucidated through traditional public health interviews and identify opportunities for prevention in rapidly growing clusters of HIV infections in states with lower HIV morbidity. (cdc.gov)
  • HIV sequence data, generated from provider-ordered drug resistance testing, can be used to understand characteristics of molecular clusters, a group of sequences for which each sequence is highly similar (linked) to all other sequences, and assess whether named partners are plausible HIV transmission partners. (cdc.gov)
  • Molecular HIV clusters provide evidence of rapid transmission. (cdc.gov)
  • Genomic organization was determined by alignment of contigs with published cDNA sequences and zebrafish genomic sequence from Sanger and Ensembl databases. (zfin.org)
  • Polymerase chain reactions (PCR) and Sanger sequencing were performed on samples from one pup. (vin.com)
  • Computer scientist Tandy Warnow, biologist Randy Linder and their graduate students have created an automated computing method, called SATé, that can analyze these molecular data from thousands of organisms, simultaneously figuring out how the sequences should be organized and computing their evolutionary relatedness in as little as 24 hours. (sciencedaily.com)
  • The sequencing reactions were performed by using ABI Prism™ Big Dye™ Terminator Cycle Sequencing Ready Reaction Kit v1.1 (Applied Biosystems). (uni-goettingen.de)
  • Lab technicians worked together to test samples, identify candidates for WGS, and ultimately perform the sequencing reactions. (health.mil)
  • Oftentimes the only way to capture these uncommon sequences is to use the value-added features of the sequence databases on STN. (piug.org)
  • Software and Databases for Molecular Evolutionary Biology. (who.int)
  • Public Health Approach to Big Data in the Age of Genomics: How Can we Separate Signal from Noise? (cdc.gov)
  • Reference-free population genomics from next-generation transcriptome data and the vertebrate-invertebrate gap. (who.int)
  • Tsagkogeorga G, Cahais V, Galtier N. The population genomics of a fast evolver: high levels of diversity, functional constraint, and molecular adaptation in the tunicate Ciona intestinalis. (who.int)
  • Caris provides artificial intelligence integrated molecular science technology that assesses deoxyribonucleic acid, Ribonucleic acid, and proteins to reveal a molecular blueprint that allows physicians and cancer patients to make personalized treatment decisions. (cbinsights.com)
  • In my group we use and develop bioinformatics tools for analysing molecular sequence data. (lu.se)
  • MeSH is available in RDF Linked Data format. (bvsalud.org)
  • 2019) based on molecular sequence data. (wikipedia.org)
  • Graph generated 31 August 2019 using data from PubMed using criteria . (cancerindex.org)
  • The digital health collection includes vendors developing software, platforms, sensor & robotic hardware, health data infrastructure, and tech-enabled services in healthcare. (cbinsights.com)
  • Benchmarking second and third-generation sequencing platforms for microbial metagenomics. (cdc.gov)
  • Antibiotic resistance surveillance of Klebsiella pneumoniae complex is affected by refined MALDI-TOF identification, Swiss data, 2017 to 2022. (cdc.gov)
  • therefore, this report is based on preliminary data for 2022. (medscape.com)
  • Based on preliminary data, 29 meningococcal disease cases have been reported among persons with HIV in 2022, accounting for 9.8% of all cases. (medscape.com)
  • Data for 2022 are not yet final, and these numbers might increase when reporting is complete for the year. (medscape.com)
  • Of the 14 cases among persons with HIV in 2022 that were not related to the outbreak primarily among MSM, nine were caused by a single strain of N. meningitidis serogroup Y clonal complex CC174 sequence type ST-1466. (medscape.com)
  • Data from experiments will be utilized by the mathematical members to both formulate and calibrate mathematical models of bacterial movement. (clarkson.edu)
  • For their paper, Warnow, Linder and their students tested SATé using computer-generated data and real biological data. (sciencedaily.com)
  • The biological data had been previously aligned manually by other experts. (sciencedaily.com)
  • HIV sequence data derived from standard drug resistance testing are reportable by laboratories to the Wisconsin DPH and are maintained in a secure surveillance database. (cdc.gov)
  • The Armed Forces Health Surveillance Division Global Emerging Infections Surveillance's Next Generation Sequencing and Bioinformatics Consortium supported their efforts. (health.mil)
  • Activities included review of surveillance and partner services interview data, † medical chart reviews, and qualitative interviews with service providers and Hispanic MSM community members. (cdc.gov)
  • Olfactory receptors are supposed to act not only as molecular sensors for odorants but also as cell recognition molecules guiding the axons of olfactory neurons to their appropriate glomerulus in the olfactory bulb. (jneurosci.org)
  • HIV-1 sequence data reported in Wisconsin during 2014-2017 for persons with HIV infection diagnosed through August 15, 2017, were analyzed using Secure HIV-TRACE (Secure HIV TRAnsmission Cluster Engine). (cdc.gov)
  • Multilocus sequence typing at 13 loci revealed that all were infected with the same strain of T. gondii , previously characterized as an atypical and rare genotype in North America (TgCgCa1, or COUG). (frontiersin.org)
  • Epidemiologic and genomic data suggest spread from Asia. (cdc.gov)
  • Established in the mid 1970's, New England Biolabs, Inc. is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. (neb.com)
  • The QIAseq Targeted RNA Panel (12) is intended for molecular biology applications. (qiagen.com)
  • QIAseq Targeted RNA Panels and Indices are intended for molecular biology applications. (qiagen.com)
  • Several downstream analyses are performed and their utility in applied ecology, evolutionary biology and molecular biology research will be discussed with guest speakers. (lu.se)
  • Mathematical members will utilize both mechanistic principles and individual cell data to discover interaction rules in many-particle biological systems that depend on both local interactions and environmental factors. (clarkson.edu)
  • Targeted RNA sequencing using UMIs is optimized to deliver highly reliable quantification down to ~100 copies of an RNA target in 25 ng total RNA (see figure Positive results with as little as 0.2 copies of RNA per cell ERCC standards, at 86 to 705,500 copies, spiked into universal reference RNA sample and enriched using 384-plex QIASeq Targeted RNA Panel in three technical replicates. (A) Sensitivity measurement. Under standard conditions (20 ng RNA input, 0.5 million MiSeq reads), ≥~100 copies of ERCC transcripts were reliability detected, which is the equivalent of ~0.2 copies per cell. (B) Precision measurement. At >10 barcodes/gene, CV was less than 5% for all targets, indicating high technical reproducibility. This corresponds to ~100 copies target RNA in the sample. "> Positive results with as little as 0.2 copies of RNA per cell ). (qiagen.com)
  • My research utilizes HIV-1 sequence data to provide insights into HIV-1 diversity, transmission patterns, past-population dynamics, and transmitted drug resistance trends in Kenya. (lu.se)
  • Discovery of this second putative receptor site provides a dual-receptor paradigm that could explain much of the molecular mechanisms of pyrethroid action and resistance as well as the high selectivity of pyrethroids on insect vs. mammalian sodium channels. (duke.edu)
  • I have a continued interest in understanding the molecular mechanisms that underlie pathogenesis and spread of human diseases. (lu.se)
  • A tabular approach to the sequence-to-structure relation in proteins (tetrapeptide representation) for de novo protein design. (medscimonit.com)
  • Editor's note: This is the sixth installment in a 7-part series that highlights the work of the Military Health System laboratories and the technicians who worked to identify COVID-19 variants using special sequencing technology. (health.mil)
  • DiaSorin Molecular noted that the Simplexa COVID-19 Direct kit detects SARS-CoV-2 RNA, including in the new variants of the coronavirus recently isolated in the UK and South Africa. (genomeweb.com)
  • In many fields of knowledge, recent advances in information technology, along with the democratization of computing, expansion of computer networks and proliferation of information sources, have directly resulted in massive production of data. (fapesp.br)
  • All these advances have left researchers with a new problem: how to go about processing, organizing and viewing the avalanche of data obtained through the most diverse means. (fapesp.br)
  • Figuring out these relationships requires analyzing large amounts of molecular data, such as DNA and protein sequences. (sciencedaily.com)
  • The idea of connecting traditional scientific practice with the access, use and processing of large amounts of data will change the way we do science and increase its potential. (fapesp.br)
  • One of the principal barriers we could face is communication problems among scientists on the teams needed to do science in this way, which is heavily based on data or large amounts of data. (fapesp.br)