• Molecular & Cellular Proteomics. (wikipedia.org)
  • We also examined the dataset of proteins obtained by the quantitative proteomics analysis using two tools, Downstream Effect Analysis and Upstream Regulator Analysis of the Ingenuity Pathways Analysis software. (oncotarget.com)
  • Redox proteomics has taken advantage of the thiol specificity of ICAT reagents not only to identify targets of ROS but also to quantify oxidative thiol modifications in individual proteins. (hindawi.com)
  • They use quantitative proteomics, employing stable isotope labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) but also use other high-throughput methods, such as high content screening and massively parallel sequencing. (cnpn.ca)
  • Targeted proteomics and absolute protein quantification for the construction of a stoichiometric host-pathogen surface density model. (malmstroem.net)
  • Although proteomics isn't exactly stuck-it is chugging along thanks to mass spectrometry (MS)-it sometimes spins its wheels because the world of proteins is particularly slippery. (genengnews.com)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • Proteins from both cell populations are combined and analyzed together by mass spectrometry as pairs of chemically identical peptides of different stable-isotope composition can be differentiated in a mass spectrometer owing to their mass difference. (wikipedia.org)
  • The results suggest that polyclonal antibodies generated via immunization of recombinant protein fragments could be used for the enrichment of target peptides to allow for rapid mass spectrometry analysis taking advantage of a substantial reduction in sample complexity. (nih.gov)
  • Our method allows for the identification of the proteins, identification of redox-sensitive cysteines within proteins, and quantification of the redox status of individual cysteine-containing peptides. (hindawi.com)
  • The workflow relies on stable isotope labelled reference peptides and selected reaction monitoring mass spectrometry analysis of a wild-type strain and an M-protein deficient mutant strain to generate absolutely quantified protein stoichiometry ratios between S. pyogenes and interacting plasma proteins. (malmstroem.net)
  • Peptides can be labeled with various trivalent radiometals for imaging or targeted radionuclide-therapy applications. (nature.com)
  • 2011. Complete Chemical Modification of Amine and Acid Functional Groups of Peptides and Small Proteins . (wisc.edu)
  • And should a tighter focus be desired, there are techniques that can peer into niches that harbor elusive forms such as small peptides or post-translationally modified proteins. (genengnews.com)
  • However, the reproducibility of enzymatic digestion of proteins to produce proteotypic target peptides is a major limiting factor of assay precision. (cdc.gov)
  • We identified 231 proteins released from actomyosin contraction-dependent blebs and apoptotic bodies by adapted SILAC (stable isotope labeling with amino acids in cell culture) combined with mass spectrometry analysis. (nature.com)
  • Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. (wikipedia.org)
  • In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy isotopes. (wikipedia.org)
  • Pulsed SILAC (pSILAC) is a variation of the SILAC method where the labelled amino acids are added to the growth medium for only a short period of time. (wikipedia.org)
  • The NeuCode amino acid method is similar to SILAC but differs in that the labeling only utilizes heavy amino acids. (wikipedia.org)
  • The increased multiplexing capability of NeuCode amino acids is from the use of mass defects from extra neutrons in the stable isotopes. (wikipedia.org)
  • We investigated the entire set of proteins modulated by BAG3 silencing in the human anaplastic thyroid 8505C cancer cells by using the Stable-Isotope Labeling by Amino acids in Cell culture strategy combined with mass spectrometry analysis. (oncotarget.com)
  • Cysteines are one of the most rarely used amino acids, but when conserved in proteins they often play critical roles in structure, function, or regulation. (hindawi.com)
  • His Chromatography study incorporates themes from Adipose tissue, Stable isotope labeling by amino acids in cell culture and Trypsin. (research.com)
  • We used two independent discovery-based approaches-microarray gene expression and stable isotope labeling by amino acids in cell culture (SILAC) quantitative proteomic mass spectrometry analyses-to identify cellular phenotypes in SZ hiPSC NPCs from four SZ patients. (ca.gov)
  • This study aimed to examine the proteomic response to DMXAA and unveil the global molecular targets and possible mechanisms for the anticancer effect of DMXAA in NSCLC A549 cells using a stable-isotope labeling by amino acids in cell culture (SILAC) approach. (elsevierpure.com)
  • In: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols, pp. 65 - 83. (mpg.de)
  • To quantitatively assess the molecular composition of HCT-containing signaling endosomes, we have developed a protocol for triple Stable Isotope Labeling with Amino acids in Cell culture (SILAC) in embryonic stem cell-derived motor neurons. (figshare.com)
  • The building blocks of protein are amino acids. (nucdf.org)
  • Citrullinemia type I, a urea cycle defect caused by a deficiency of argininosuccinate synthetase, was diagnosed on the basis of plasma amino acids and was confirmed by molecular testing. (nucdf.org)
  • Here, we describe a new method (immuno-SILAC) for the absolute quantification of proteins in complex samples based on polyclonal antibodies and stable isotope-labeled recombinant protein fragments to allow affinity enrichment prior to mass spectrometry analysis and accurate quantification. (nih.gov)
  • Protein digestion coupled to liquid chromatography and tandem mass spectrometry (LC-MS/MS) detection enables multiplexed quantification of proteins in complex biological matrices. (cdc.gov)
  • A SILAC approach involving incorporation of tyrosine labeled with nine carbon-13 atoms (13C) instead of the normal carbon-12 (12C) has been utilized to study tyrosine kinase substrates in signaling pathways. (wikipedia.org)
  • SILAC has emerged as a very powerful method to study cell signaling, post translation modifications such as phosphorylation, protein-protein interaction and regulation of gene expression. (wikipedia.org)
  • In addition, SILAC has become an important method in secretomics, the global study of secreted proteins and secretory pathways. (wikipedia.org)
  • It had also been used to study biofilm tolerance to antibiotics to differentiate tolerant and sensitive subpopulations Traditionally the level of multiplexing in SILAC was limited due to the number of SILAC isotopes available. (wikipedia.org)
  • Recently, a new technique called NeuCode (neutron encoding) SILAC, has augmented the level of multiplexing achievable with metabolic labeling (up to 4). (wikipedia.org)
  • Collectively, this SILAC study quantitatively evaluates the proteomic response to treatment with DMXAA that helps to globally identify the potential molecular targets and elucidate the underlying mechanism of DMXAA in the treatment of NSCLC. (elsevierpure.com)
  • Proteins in cells with or without DN R-Ras expression were differentially labeled with SILAC and mass spectrometry was used to identify phosphoproteins and determine their relative quantities in the presence and absence of DN R-Ras. (biomedcentral.com)
  • The approach was further developed to also generate quantitative data via the addition of heavy isotope-labeled recombinant protein fragment standards prior to trypsin digestion. (nih.gov)
  • Here, we show that we were able to use small amounts of antibodies (50 ng per target) in this manner for efficient multiplex analysis of quantitative levels of proteins in a human HeLa cell lysate. (nih.gov)
  • Dr. Terry Cyr , Health Canada, Ottawa, ON " Quantitative analysis of Influenza Vaccine Antigens: How Does the Hi3 Method Compare to Methods Requiring Labeled Isotopes? (cfabs.org)
  • This approach used the stable heavy isotope-based labeling of the unmodified lysine residues with acetyl groups, followed by deep proteomic profiling and the use of a novel in-house-developed software program for quantitative analysis for stoichiometry determination. (genengnews.com)
  • In proteins where cysteine is not within the active site, activity can be modulated by changing conformation or by influencing its regulatory role, for example, iron sulfur complexes (ISCs) in aconitase possess cysteines required for its activity [ 7 ]. (hindawi.com)
  • In this research field we focus on the development of robust chemical tools that can be used in integrative structural biology approaches that target ribonucleoprotein (RNP) particles or RNA protein complexes with high molecular weight in general. (uibk.ac.at)
  • In detail, we want to introduce the methyl TROSY technique in combination with deuteration to nucleic acid NMR spectroscopy to expand the addressable molecular weight of RNA protein complexes. (uibk.ac.at)
  • We further want to simplify the application of PRE NMR in nucleic acid protein complexes. (uibk.ac.at)
  • The use of methyl TROSY techniques, in conjunction with specialized isotopic labeling, have significantly increased the useful molecular weight range available for NMR investigations for the study of large proteins and supramolecular protein complexes. (isotope.com)
  • Alteration of mitochondrial protein complexes in relation to metabolic regulation under short-term oxidative stress in Arabidopsis seedlings. (mpg.de)
  • The peptide is first conjugated to a chelating agent that is able to form stable complexes with the radionuclide of interest. (nature.com)
  • 2011. Sequence-Specific Capture of Protein-DNA Complexes for Mass Spectrometric Protein Identification . (wisc.edu)
  • Proteomic discovery of chemical probes that perturb protein complexes in human cells. (ucsd.edu)
  • And proteins form complexes and participate in protein networks that are variable over time and space. (genengnews.com)
  • For example, there are new methods that could keep protein-protein complexes from falling apart before they are detected. (genengnews.com)
  • In his study, which falls under the umbrella issue of Proteome, Molecular biology is strongly linked to Cancer cell. (research.com)
  • His Molecular biology study frequently links to other fields, such as Recombinant DNA. (research.com)
  • Leonard Foster, PhD, is a Professor and the Chair of the Biochemistry and Molecular Biology department at the University of British Columbia. (cnpn.ca)
  • Jennifer Geddes-McAlister, PhD, is an assistant professor in the Department of Molecular and Cellular Biology at the University of Guelph. (cnpn.ca)
  • Their tiny size, rapid growth by clonal propagation, and facile uptake of labeled compounds from the media were attractive features that made them a well-known model for plant biology from 1950 to 1990. (osti.gov)
  • Molecular Biology of the Cell. (hutton.ac.uk)
  • Challenges in studying protein post-translational modifications extend to both identification and quantitation," says Yue Chen, Ph.D., assistant professor of biochemistry, molecular biology, and biophysics at the University of Minnesota. (genengnews.com)
  • Molecular Membrane Biology (2013), 30 (1): 75-89. (ezreregister.com)
  • Cell surface sensors on immune cells that ordinarily recognize pathogen-associated molecular patterns produced by infectious agents may also interact with DAMPs, leading to the activation of signaling pathways resulting in a nonpathogen-induced 'sterile' inflammatory response. (nature.com)
  • Ingenuity pathway analysis (IPA) identified 256 signaling pathways and 184 cellular functional proteins that were regulated by DMXAA in A549 cells. (elsevierpure.com)
  • R-Ras proteins function distinctly from classic Ras proteins by regulating distinct signaling pathways that promote MPNST tumorigenesis by mediating migration and invasion. (biomedcentral.com)
  • We have previously shown that three related Ras proteins (the classic Ras proteins) are highly activated in malignant peripheral nerve sheath tumor (MPNST) cells with neurofibromin loss and that they drive cancer cell proliferation and survival by activating multiple cellular signaling pathways. (biomedcentral.com)
  • We examined the activation of cytoplasmic signaling pathways in the presence and absence of R-Ras signaling and found that R-Ras proteins regulated 13 signaling pathways distinct from those regulated by classic Ras proteins. (biomedcentral.com)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Since the level of monomeric soluble amyloid beta as well as amyloid beta-binding molecules determine the onset and amount of amyloid beta aggregation, significant attention has been devoted to defining the molecular and systemic pathways that modulate amyloid beta synthesis and clearance in the brain. (wustl.edu)
  • We these NMR active labels powerful NMR experiments, such as the relaxation dispersion experiment or the CEST experiment can be applied to RNAs containing the very modification. (uibk.ac.at)
  • LP3 is colocalised with and closely collaborates with the DEMAX platform of the ESS on production of deuterium labeled biomolecules (proteins, lipids) for neutron scattering experiments and the development of methods for crystal growth for neutron crystallography. (lu.se)
  • It is very difficult to measure absolute stoichiometry of protein modifications in mass spectrometry experiments," notes Dr. Chen. (genengnews.com)
  • The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
  • Stable isotopes are harmless compounds that can be used to track and measure the production of specific chemicals in the body. (nucdf.org)
  • 05 In-gel digestion for mass spectrometric characterization of proteins and proteomes. (imarkerlab.com)
  • As such, we provide services and make equipment available in the areas of recombinant protein production, crystallisation, biophysical characterization, and structure determination. (lu.se)
  • LP3 and PPS offer services for the entire process chain of production, purification, characterization, protein crystallisation and protein structure determination and structure refinement, or each individual step in the chain. (lu.se)
  • The identification and characterization of protein modifications is particularly challenging for low-abundance proteins, which are often detected only after enrichment. (genengnews.com)
  • For crystallisation, the facility is equipped with state-of-the-art nanolitre pipetting equipment with capability to handle lipidic cubic phases for membrane proteins, as well as a plate hotels with the capacity to store and automatically image crystallisation plates. (lu.se)
  • Through applying cutting edge techniques like advanced light microscopy, cell-free protein synthesis and microfluidics, we seek to systematic investigation of membrane proteins as well as bottom-up assembly of minimal cellular systems. (ezreregister.com)
  • High Level Cell-Free Production of Membrane Proteins into Nanodiscs. (ezreregister.com)
  • Co-translational insertion of cell-free expressed membrane proteins into lipid bilayers. (ezreregister.com)
  • Students will learn about protein expression and purification, and measurements of binding affinity and kinetics. (tamu.edu)
  • Interactions with other proteins or molecules are another feature of cysteines that can affect protein activity. (hindawi.com)
  • Explore protein expression patterns, functional annotations, and molecular interactions across diverse biological systems. (imarkerlab.com)
  • Molecular modelling of host-pathogen interactions in the disease state represents a promising strategy to define molecular events of importance for the transition from superficial to invasive infectious diseases. (malmstroem.net)
  • Future integration of new results will improve the understanding of protein-protein interactions and their importance for bacterial virulence. (malmstroem.net)
  • LP3 is fully equipped for recombinant protein production in E. coli and insect cells (BEVS) and protein purification using state of the art chromatography systems. (lu.se)
  • Protein Expression and Purification. (ezreregister.com)
  • The proteomic data showed that treatment with DMXAA modulated the expression of 588 protein molecules in A549 cells, with 281 protein molecules being up regulated and 306 protein molecules being downregulated. (elsevierpure.com)
  • Inhibition of R-Ras/R-Ras2 and ROCK1 signaling also triggered the accumulation of abnormal intracellular vesicles, indicating that these signaling molecules regulate the movement of proteins and other molecules in the cellular interior. (biomedcentral.com)
  • The structure of isotopes is slightly different from other molecules, so by giving a small amount through an infusion into a vein, researchers are able follow them as they go through the urea cycle. (nucdf.org)
  • Molecular numbers of apoA-I, apoA-II, and eight major lipid classes were determined in each subpopulation by LC/MS. The average number of lipid molecules decreased from 422 in the large spherical alpha-1 particles to 57 in the small discoid prebeta-1 particles. (cdc.gov)
  • A protocol based on the simultaneous multiplex capture of more than 40 protein targets showed that approximately half of the antibodies enriched at least one functional peptide detected in the subsequent mass spectrometry analysis. (nih.gov)
  • This has further paved the path for functional analysis of the loci by VIGS to understand the molecular mechanism of resistance. (bvsalud.org)
  • Indeed, it has been demonstrated that cysteines are characterized by the most extreme conservation pattern, being highly conserved in functional positions of proteins but poorly conserved otherwise [ 5 ]. (hindawi.com)
  • His research has covered all aspects of human nutrition, with a strong focus on the use of stable isotopes to determine body composition and functional capability at the level of individual tissues and the whole body. (southampton.ac.uk)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • The first applications of this technology involved exposing purified proteins to either OS or normal condition before labeling with either heavy or light ICAT reagents, respectively. (hindawi.com)
  • 09 Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. (imarkerlab.com)
  • Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. (nature.com)
  • 2 , 3 A key feature of apoptosis has been defined as the maintenance of an intact cellular membrane (detectable as exclusion of impermeable dyes such as propidium iodide, PI) throughout the apoptotic program to prevent intracellular protein release and subsequent immunological activation. (nature.com)
  • Mass spectrometry-based phosphoproteomics identified thirteen protein networks distinctly regulated by DN R-Ras, including multiple networks regulating cellular movement and morphology. (biomedcentral.com)
  • Closer study of an R-Ras regulated pathway containing the signaling protein ROCK1 showed that inhibition of either R-Ras, R-Ras2 or ROCK1 similarly impaired cellular migration and invasion and altered cellular morphology. (biomedcentral.com)
  • Since the last seven years the research group Kreutz strongly focused on the synthesis of RNA phosphoramidite building blocks bearing site-specific 2 H, 13 C and 15 N labels. (uibk.ac.at)
  • The use of solid phase synthesis for NMR of RNA simplifies the interpretation of the NMR data as the number and positioning of the stable isotope labels can be freely be chosen. (uibk.ac.at)
  • Systems for the Cell-Free Synthesis of Proteins. (ezreregister.com)
  • The introduction of 13 CH 3 groups in a perdeuterated protein sample may be achieved by several methods. (isotope.com)
  • N-glycosylation profoundly affects the biological stability and function of therapeutic proteins, which explains the recent interest in glycoengineering technologies as methods to develop biobetter therapeutics. (umd.edu)
  • They include labeling, separation, and extraction methods that can preserve contextual information. (genengnews.com)
  • Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)
  • The model outlines the topology and density of the host-pathogen protein interaction network on the S. pyogenes bacterial surface revealing a dense and highly organized protein interaction network. (malmstroem.net)
  • Botulinum neurotoxins (BoNTs) are bacterial proteins that cause the life-threatening disease botulism 1 . (cdc.gov)
  • Here we used the gram-positive bacterium Streptococcus pyogenes as a model system to establish a mass spectrometry based workflow for the construction of a stoichiometric surface density model between the S. pyogenes surface the surface virulence factor M-protein and adhered human blood plasma proteins. (malmstroem.net)
  • To measure the clearance of apoE and amyloid beta in the mouse brain, we developed a technique that couples stable isotope-labeling kinetics (SILK) with mass spectrometry. (wustl.edu)
  • It can be used to distinguish between proteins secreted by cells in culture and serum contaminants. (wikipedia.org)
  • These include the use of value-assigned serum as calibrators and stable isotope-labeled (SIL) peptide analogs as internal standards. (cdc.gov)
  • Chaudhary-Webb M, Schleicher RL, Erhardt JG, Pendergrast EC, Pfeiffer CM. An HPLC ultraviolet method using low sample volume and protein precipitation for the measurement of retinol in human serum suitable for laboratories in low- and middle-income countries external icon . (cdc.gov)
  • Esmaeili R, Zhang M, Sternberg MR, Mapango C, Pfeiffer CM . A new multiplex immunoassay for serum ferritin, soluble transferrin receptor, C-reactive protein, α-1-glycoprotein, and retinol-binding protein showed variable comparability with traditional reference assays external icon . (cdc.gov)
  • Fazili Zand Pfeiffer CM. Accounting for an isobaric interference allows correct determination of folate vitamers in serum by isotope dilution-liquid chromatography-tandem mass spectrometry external icon . (cdc.gov)
  • Fazili Z, Pfeiffer CM. Measurement of folates in serum and conventionally prepared whole blood lysates: application of an automated 96-well plate isotope-dilution tandem MS method external icon . (cdc.gov)
  • McCoy LF, Scholl PF, Schleicher RL, Groopman JD, Powers CD, Pfeiffer CM. Analysis of aflatoxin B1-lysine adduct in serum using isotope-dilution liquid chromatography/tandem mass spectrometry external icon . (cdc.gov)
  • Mineva EM, Schleicher RL, Chaudhary-Webb, M, May KL, Botelho JC, Vesper HW, Pfeiffer CM. A candidate reference measurement procedure for quantifying serum concentrations of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 using isotope-dilution liquid chromatography-tandem mass spectrometry external icon . (cdc.gov)
  • Pawlosky RJ, Flanagan VP, Pfeiffer CM. Determination of 5-methyltetrahydrofolic acid in human serum by stable-isotope dilution high-performance liquid chromatography-mass spectrometry external icon . (cdc.gov)
  • Our analyses confirm the association of the proteome profile observed in BAG3-silenced cells with an increase in cell survival and a decrease in cell proliferation and invasion, and highlight the possible involvement of four tumor suppressor miRNAs and TP53/63 proteins in BAG3 activity. (oncotarget.com)
  • His Secretory protein study integrates concerns from other disciplines, such as Proteome, Receptor, Granule and Mutant. (research.com)
  • 03 MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. (imarkerlab.com)
  • Many of these proteins are reportedly involved in tumor progression, invasiveness and resistance to therapies. (oncotarget.com)
  • We focused our attention on an oncogenic protein, CAV1, and a tumor suppressor protein, SERPINB2, that had not previously been reported to be modulated by BAG3. (oncotarget.com)
  • This rare thyroid tumor does not respond to any chemotherapeutic regimen or radioiodine treatment thus representing a candidate target for innovative therapies, such as molecular targeted therapy [ 2 ]. (oncotarget.com)
  • Loss of the Ras GTPase-activating protein neurofibromin promotes nervous system tumor pathogenesis in patients with neurofibromatosis type 1 (NF1). (biomedcentral.com)
  • We have shown that classic Ras proteins promote proliferation and survival, but not migration, in malignant peripheral nerve sheath tumor (MPNST) cells. (biomedcentral.com)
  • Contingency furniture and the chi2-test were performed to search for associations between molecular guidelines and tumor phenotype. (cancercurehere.com)
  • For zinc content, a highly significant SNP was detected in a gene encoding UPF0678 fatty acid-binding protein. (bvsalud.org)
  • The undergraduate student(s) will use these prioritized candidate genes to test their requirement for mitochondrial bioenergetics by placing the gene(s) in the metabolic pathway by using biochemical and molecular genetic techniques. (tamu.edu)
  • Schizophrenia brain cells showed abnormal gene expression and protein levels related to cytoskeletal remodeling and oxidative stress. (ca.gov)
  • From our findings that SZ hiPSC NPCs show abnormal gene expression and protein levels related to cytoskeletal remodeling and oxidative stress, we predicted, and subsequently observed, aberrant migration and increased oxidative stress in SZ hiPSC NPCs. (ca.gov)
  • Mutations of the NF1 gene potentially results in the activation of multiple Ras proteins, which are key regulators of many biologic effects. (biomedcentral.com)
  • 2005. An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognised in the host cytoplasm . (hutton.ac.uk)
  • This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • Overall, in this study we have identified markers, putative candidate genes and predicted putative interacting protein partners significantly associated with iron and zinc metabolism that could be utilized in future breeding studies of lentil for nutrient biofortification. (bvsalud.org)
  • Epitope mapping revealed that a majority of the polyclonal antibodies recognized multiple linear epitopes, and based on these results, a semi-automated method was developed for peptide enrichment using polyclonal antibodies immobilized on protein A-coated magnetic beads. (nih.gov)
  • The summer research project in the Gohil lab will focus on discovering evolutionarily conserved proteins that are required for mitochondrial energy metabolism. (tamu.edu)
  • The ratio of peak intensities in the mass spectrum for such peptide pairs reflects the abundance ratio for the two proteins. (wikipedia.org)
  • Improvements in mass spectrometry has led to the development of a number of techniques to quantify the relative protein abundance within a given sample. (hindawi.com)
  • Elemental formula annotation of polar and lipophilic metabolites using 13C, 15N and 34S isotope labelling, in combination with high-resolution mass spectrometry. (mpg.de)
  • The stoichiometry ratios in combination with a novel targeted mass spectrometry method to measure cell numbers enabled the construction of a stoichiometric surface density model using protein structures available from the protein data bank. (malmstroem.net)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • 2005. Arabidopsis nucleolar protein database (AtNoPDB) . (hutton.ac.uk)
  • When protein is broken down, a substance called nitrogen is formed. (nucdf.org)
  • In a study completed in August of 2009, stable isotopes were used to measure how well participants' bodies were able to change nitrogen to urea. (nucdf.org)
  • This molecular mimicry also helps in the continued existence of H pylori in the unfavorable gastric environment. (medscape.com)
  • The Molecular Chaperone CCT Sequesters Gelsolin and Protects it from Cleavage by Caspase-3. (gu.se)
  • The light chain is a zinc-metalloprotease which is responsible for the cleavage of three SNARE complex proteins including SNAP-25 (Synaptosome-associated protein of 25 kDa), synaptobrevin-2 (also termed VAMP-2) and syntaxin. (cdc.gov)
  • The use of molecular breeding approaches for development of lentil genotypes biofortified with essential micro-nutrients such as iron and zinc, could serve as a promising solution to address the problem of global malnutrition. (bvsalud.org)
  • Also in development are approaches that could improve our ability to see patterns of protein localization within the cell. (genengnews.com)
  • 1 Supercoiled or high molecular weight DNA (e.g. plant genomic DNA) may require longer incubation time or higher amount of enzyme. (jenabioscience.com)
  • These data further our understanding of how proteins involved in apoE metabolism influence AD pathogenesis, and have important implications for future therapeutic strategies that target brain apoE levels and function. (wustl.edu)
  • Lipoproteins are complex molecular assemblies that are key participants in the intricate cascade of extracellular lipid metabolism with important consequences in the formation of atherosclerotic lesions and the development of cardiovascular disease. (cdc.gov)
  • The Glasner Lab investigates mechanisms of protein evolution in order to understand how the biophysical properties of proteins influence their capacity to evolve new structures and functions. (tamu.edu)
  • My laboratory uses a combination of biochemical and biophysical techniques (NMR spectroscopy, X-ray crystallography, SAXS, and fluorescence spectroscopy) to understand the structure-dynamics-function relationships in these proteins. (tamu.edu)
  • Western blotting assays demonstrated that DMXAA induced apoptosis via a mitochondria-dependent pathway and promoted autophagy, as indicated by the increased level of cytosolic cytochrome c, activation of caspase 3, and enhanced expression of beclin 1 and microtubule-associated protein 1A/1B-light chain 3 (LC3-II) in A549 cells. (elsevierpure.com)
  • Lund Protein Production Platform (LP3) is a cross-faculty expert center and user facility of Lund University (LU). (lu.se)
  • These processes are mediated by multi-modular proteins that bind to membranes in response to second messengers. (tamu.edu)
  • Light-induced Printing of Protein Structures on Membranes in vitro. (ezreregister.com)
  • These toxins inhibit the release of neurotransmitters at the neuromuscular junction by cleaving soluble NSF attachment protein receptor (SNARE) complex proteins in nerve cells, which prevents the fusion of neurotransmitter containing vesicles to cell membranes 2 . (cdc.gov)
  • LP3 is the only academic protein production platform in Sweden providing specialisation in protein production using the baculovirus expression vector system (BEVS, insect cells) and perdeuteration of biological macromolecules. (lu.se)
  • For biological systems, Q peaks were first observed in protein from different perspectives [3,7,9,13,15,21-23] , the detailed molec- powders and attributed to 1H-14N spin pairs [9], which were also ular mechanism of the ubiquitous 1H-14N Q peaks in biological sys- thought to be of critical importance for the background 1H relaxa- tems has not been established. (lu.se)
  • Reversible cysteine modifications allow for potential redox regulation of proteins. (hindawi.com)
  • Despite the essential roles of signaling endosomes in neuronal differentiation and survival, little is known about their molecular identity, dynamics, and regulation. (figshare.com)
  • We took advantage of the antibody resources publicly available from the Human Protein Atlas project covering more than 80% of all human protein-coding genes. (nih.gov)
  • Using the monoclonal antibody rituximab as a model therapeutic protein, a uniform Gal2GlcNAc2Man3GlcNAc2 (A2G2) glycoform without α-1,6-fucose, plant-specific α-1,3-fucose or β-1,2-xylose residues was produced. (umd.edu)
  • This complex then reacts with fluorescein-labeled antibody. (cdc.gov)
  • Therefore, identification of low-abundance protein modifications still depends on the availability of specific enrichment strategies. (genengnews.com)
  • In addition, the effectiveness of antitoxin treatment may vary depending on circulating toxin levels, so that rapid identification and quantification of BoNT proteins in clinical specimens and food is desirable for optimization of treatment. (cdc.gov)
  • In a typical REU project, students analyze sequence data to identify mutations that could influence the evolution of protein function, then construct the mutations, express, purify and assay the enzymes to determine the role of the mutated position. (tamu.edu)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Our research is devoted to develop disease models based on genetic mutations that cause human diseases, and to understand basic molecular pathology using these models. (umn.edu)
  • Allosterically regulated proteins that require an activator are sometimes based on a thiol exchange interaction involving cysteines, for example, pyruvate kinase uses fructose bisphosphate (FBPs) as a heterotrophic activator and it contains a cysteine in its FBP binding site [ 8 ]. (hindawi.com)
  • REU students will study the molecular interaction between host and influenza viruses. (tamu.edu)
  • Within an individual protein there may be a number of cysteines which could allow for multiple thiol modifications. (hindawi.com)
  • Thus, reversible cysteine modifications can influence protein activity and the relative quantification of the status of the thiol can potentially provide valuable insights into protein activity where the protein exists in a range of redox states. (hindawi.com)
  • Proteins may reflect splicing events or post-translational modifications. (genengnews.com)
  • The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)