• Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. (nih.gov)
  • NSP3A by taking the place of PABP on eIF4GI, is responsible for the shut-off of cellular protein synthesis. (wikipedia.org)
  • Enhanced mTORC1 signaling and protein synthesis in pathologic α-synuclein cellular and animal models of Parkinson's disease. (neurotree.org)
  • These results demonstrated that membrane protein binding to PAMPS gels occurred in a charge-dependent manner, and that ITG alpha 4 plays a crucial role in cell spreading on PAMPS gels and acts as a mechanoreceptor triggering cellular signaling leads to chondrogenic differentiation. (cheric.org)
  • Previous work has demonstrated that poly(A)-binding protein (Pab1) condenses during heat shock in yeast and disrupting Pab1 condensation impairs cellular growth during stress, indicating that stress-triggered condensation of Pab1 is a part of the adaptive stress response. (nih.gov)
  • Inhibiting host translation eventually leads to shutoff of host proteins expression and gives viruses transcripts a competitive edge for access to the cellular translation machinery. (expasy.org)
  • Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
  • Quantitative image analysis of cellular protein translocation induced by magnetic microspheres: application to the EGF receptor. (mpg.de)
  • GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies ( Molecular Function , Biological Process , and Cellular Component ), a reference, and an evidence code. (yeastgenome.org)
  • In this review, we discuss the recent advances in our understanding of the molecular and cellular mechanisms that underlie Ehrlichia host cellular reprogramming strategies that enable intracellular survival. (frontiersin.org)
  • Despite its small genome and limited number of effector proteins, Ehrlichia efficiently establishes an intracellular infection and avoids immune defenses in vertebrate and invertebrate hosts through complex molecular and cellular reprogramming strategies. (frontiersin.org)
  • Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. (uiowa.edu)
  • In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
  • In NSEC research at RPI, silica nanoparticles have been used as carriers to deliver into the cell cytoplasm a variety of proteins that can influence cellular behavior. (rpi.edu)
  • PCBP1 along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. (prospecbio.com)
  • An unusual cellular factor potentiates protein-DNA complex assembly between Oct-1 and Vmw65. (mcmaster.ca)
  • These data identify a molecular mechanism controlling cellular levels of BER enzymes and correspondingly the efficiency and capacity of BER. (ox.ac.uk)
  • Poly-ADP-ribosylation is a post-translational modification catalyzed by PARP enzymes with roles in transcription and chromatin biology. (nature.com)
  • DNA was eluted in In this study we report the frequency of 100 µL molecular biology grade water and the CCR5 -delta 32 mutation in the Lebanese stored at -20 °C. DNA concentrations population. (who.int)
  • However with the rapid advances in biochemistry/molecular biology the colloid aspect of the fi eld was largely forgotten and colloid science was for a long period primarily a discipline for technical applications. (lu.se)
  • Basic concepts of colloid science like the surface force issue has wide applications in biochemistry and molecular biology. (lu.se)
  • Since the two central β-strands are used for poly(A) oligonucleotide binding, the other face of the protein is free for protein-protein interactions. (wikipedia.org)
  • Conformational epitopes often remain inaccessible to antibodies within crowded molecular complexes, whereas off-target interactions may lead to false-positive results in complex tissue preparations. (nature.com)
  • Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET). (mpg.de)
  • Mechanisms by which E. chaffeensis establishes intracellular infection, and avoids host defenses are not well understood, but involve functionally relevant host-pathogen interactions associated with tandem and ankyrin repeat effector proteins. (frontiersin.org)
  • Thus, E. chaffeensis is an excellent model organism to study host-pathogen interactions and to understand the molecular pathobiology of obligately intracellular microbes. (frontiersin.org)
  • This suggested to us that the nuclear inclusions are probably protective by sequestering the glutamine-expanded protein and reducing its native interactions. (bcm.edu)
  • Recognizing the importance of protein interactions for mediating SCA1 pathogenesis, and given that SCA1 shares clinical and pathological features with several other inherited ataxias, we proposed that understanding the interactions of Ataxin-1 as they relate to the interactions of proteins implicated in other ataxias might provide better insight about molecular mechanisms leading to Purkinje cell degeneration and ataxia. (bcm.edu)
  • Overall comparison of the model of biglycan to the recently determined x-ray structure of the same protein returns a very low Root Mean Square Deviation (RMSD), which confirms the viability of the model and its reliability as a platform for the study biglycan interactions. (jmolbiochem.com)
  • Project Summary/Abstract When cells encounter heat, they undergo a number of archetypal intracellular changes: global attenuation of translation, synthesis of molecular chaperones, and the formation of intracellular protein aggregates. (nih.gov)
  • Here, I propose to investigate a connection between heat-induced condensation and translation of molecular chaperones. (nih.gov)
  • Our analysis of SCA1 transgenic mice revealed that the mutant protein aggregates in the nuclei of cerebellar Purkinje cells and that these aggregates stain positively for ubiquitin, the proteasome, and some chaperones. (bcm.edu)
  • Poly(A)-binding protein is also present during stages of mRNA metabolism including nonsense-mediated decay and nucleocytoplasmic trafficking. (wikipedia.org)
  • We are using these tools to investigate poly P metabolism and functions in fungi, plants and algae. (openwetware.org)
  • However, at the moment our main efforts are devoted to the study of poly P metabolism in the yeast Saccharomyces cerevisiae and of poly P in fungal cell walls. (openwetware.org)
  • 0.22-µm polyether sulfone (PES) low-protein-binding other mechanisms (7). (cdc.gov)
  • Current research questions range from mechanisms and functions of subcellular protein localization in axonal development and functions to the molecular mechanisms of yippee-like genes in synapse development. (unr.edu)
  • Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
  • Although these findings demonstrate the anticancer activity of terpinen-4-ol, the underlying molecular mechanisms of the antitumor activity of terpinen-4-ol remain unclear. (hindawi.com)
  • The possible molecular mechanisms responsible for its anticancer activity were also investigated. (hindawi.com)
  • By immobilizing specific proteins, it is possible to influence various components of the cell and dictate cell function and fate, as well as enable detection of specific intracellular mechanisms for further study. (rpi.edu)
  • Respiratory sensitizers, such as natural proteins or low-molecular-weight reactive chemicals acting as haptens, may induce occupational asthma through immunoglob- ulin E (IgE)-dependent mechanisms. (cdc.gov)
  • Ultrafree-4 centrifugal filter units of Biomax-PB poly- duction (8) and genes likely linked to generalized trans- ethersulfone membranes with a molecular weight cutoff of duction (9). (cdc.gov)
  • Analyzing 5′-upstream non-protein-encoding regions of the human mitochondrial function-associated genes, we speculate that mitochondrial functions could be recovered or improved at a transcriptional level. (intechopen.com)
  • In Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, edn 2. (jmolbiochem.com)
  • However, conventional ensemble approaches that are widely used in anatomy, biochemistry, and pharmacology for the quantification of molecular changes are limited in their capabilities to capture the multiscale level of immense diversity in the brain and in other organs. (nature.com)
  • Poly (Ethylene) Glycol (PEG) Precipitation of Glycosylated and Non-Glycosylated Monoclonal Antibodies," Process Biochemistry , vol. 121, pp. 563 - 574, Elsevier, Oct 2022. (mst.edu)
  • The binding protein is also involved in mRNA precursors by helping polyadenylate polymerase add the poly(A) nucleotide tail to the pre-mRNA before translation. (wikipedia.org)
  • In our present view, these areas are found for systems where several types of macromolecules (proteins, polysaccharides or polyelectrolytes) and/or amphiphiles interact to form a functional or destructive unit. (lu.se)
  • The nuclear isoform selectively binds to around 50 nucleotides and stimulates the activity of polyadenylate polymerase by increasing its affinity towards RNA. (wikipedia.org)
  • It shares homology with the mating-type protein, Mc, from the fission yeast Schizosaccharomyces pombe and a conserved DNA-binding motif present in the nuclear high-mobility-group proteins HMG1 and HMG2. (nih.gov)
  • Overall, our results suggest that poly-PR might cause length-dependent defects in cargo loading, cargo release, Kapβ transport and Ran gradient across the nuclear envelope. (rug.nl)
  • Jafarinia, H , Van der Giessen, E & Onck, PR 2022, ' Molecular basis of C9orf72 poly-PR interference with the β-karyopherin family of nuclear transport receptors ', Scientific Reports , vol. 12, nr. 1, 21324. (rug.nl)
  • A viral nuclear noncoding RNA binds re-localized poly(A) binding protein and is required for late KSHV gene expression. (uiowa.edu)
  • The poly(A) tail is located on the 3' end of mRNA and was discovered by Mary Edmonds, who also characterized the poly-A polymerase enzyme that generates the poly(a) tail. (wikipedia.org)
  • Poly(ADP-ribose) polymerase 1 is inhibited by a histone H2A variant, MacroH2A, and contributes to silencing of the inactive X chromosome. (nature.com)
  • Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
  • The scaffold protein XRCC1, DNA polymerase beta, and DNA ligase IIIalpha play pivotal roles in BER. (ox.ac.uk)
  • This binding forms the characteristic loop structure of eukaryotic protein synthesis. (wikipedia.org)
  • A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons. (uiowa.edu)
  • Rotavirus mRNAs terminate a 3' GACC motif that is recognized by the viral protein NSP3. (wikipedia.org)
  • Site-specific cleavage of the host poly(A) binding protein by the encephalomyocarditis virus 3C proteinase stimulates viral replication. (ucsb.edu)
  • This valuable study reports multi-scale molecular dynamics simulations to investigate a class of highly potent antibodies that simultaneously engage with the HIV-1 Envelope trimer and the viral membrane. (elifesciences.org)
  • PCBP1 is a single-stranded nucleic acid binding protein that binds preferentially to oligo dC. (prospecbio.com)
  • Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. (nih.gov)
  • The expression of mammalian poly(A)-binding protein is regulated at the translational level by a feed-back mechanism: the mRNA encoding PABP contains in its 5' UTR an A-rich sequence which binds poly(A)-binding protein. (wikipedia.org)
  • 2022) " Dual Leucine Zipper Kinase Regulates Dscam Expression through a Noncanonical Function of the Cytoplasmic Poly(A)-Binding Protein " J Neurosci. (unr.edu)
  • The prevailing method for molecular imaging is antibody-based immunolabeling. (nature.com)
  • These studies show that glycosylation increases the solubility of the antibody and that models based on excluded volume principles or on the statistical correlation of solubilities are unable to capture the effect of glycosylation on protein precipitation by PEG. (mst.edu)
  • Ancillary protein-lipid contacts reveal surprising contributions from antibody framework regions. (elifesciences.org)
  • The modeling platform developed here uncovers insights into lipid participation in antibodies' recognition of membrane proteins and highlights antibody features to prioritize in vaccine design. (elifesciences.org)
  • [ 1 , 2 ] PEG, a water-soluble linear polymer, appears to accelerate antibody-RBC binding by steric exclusion of water molecules in the diluents and to promote antibody detection. (medscape.com)
  • AHG is an animal antibody that binds to the F c portion of human immunoglobulin. (medscape.com)
  • The AHG detects bound RBC antibodies that do not produce direct agglutination (sensitizing antibody). (medscape.com)
  • The presence of agglutination with the addition of AHG indicates antibody binding to a specific red cell antigen. (medscape.com)
  • Cytosolic poly-A binding protein (PABPC) is made up of four RNA recognition motifs (RRMs) and a C-terminal region known as the PABC domain. (wikipedia.org)
  • Moreover, speci®c hy- found in narrow minor groove regions in a variety dration patterns seem to play a role in nucleotide sequence recognition by proteins (Otwinowski of oligonucleotide duplexes (Prive et al. (lu.se)
  • They are thought to be structurally and functionally different from poly-A binding proteins found in the cytosol. (wikipedia.org)
  • Antibodies can label immature or inactive proteins hence their usefulness to quantify functionally relevant pools of signaling molecules is also limited. (nature.com)
  • Here we demonstrate that RNA-binding proteins can be used to isolate mRNA populations representing total cell mRNA from specific types of cells, as well as discrete mRNA subpopulations that represent post-transcriptionally regulated subsets of mRNAs that encode functionally related proteins. (biomedcentral.com)
  • Moreover, because many different mRNA-binding proteins in specific cell types are known to interact with unique subpopulations of mRNAs encoding functionally related proteins [ 9 - 15 ] they can be informative of the dynamic effects of cells on one another. (biomedcentral.com)
  • Systems that previously were considered too complex for a molecular characterization now successively become available to fundamental studies. (lu.se)
  • It is ranging from cloning and molecular development to production and characterization. (lu.se)
  • 8. von Schantz L, Schagerlöf H, Nordberg-Karlsson E, Ohlin M. ( 2014 ) Characterization of the substitution pattern of cellulose derivatives using carbohydrate-binding modules. (lu.se)
  • The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. (nih.gov)
  • Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
  • Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
  • Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)
  • Human molecular genetics 26.1 (2017 Jan 1): 192-209. (uiowa.edu)
  • To visualize new target proteins, a major drawback is usually the lack of sensitive and specific antibodies. (nature.com)
  • Antibodies can target epitopes on integral membrane proteins very near to the lipid bilayer surface, even those partially embedded within the headgroup region. (elifesciences.org)
  • Significant facilities for the generation of poly, mono and recombinant antibodies. (dcu.ie)
  • After immediate spin, the tubes are incubated at 37°C. To promote the detection of warm reactive antibodies, especially of the IgG class, additional enhancement techniques such as low ionic strength saline (LISS) and polyethylene glycol (PEG) are often used. (medscape.com)
  • The structural and functional integrity of this overhang is based on its association with a constellation of specific proteins, some of them belonging to the shelterin complex [ 10 ]. (hindawi.com)
  • powerful alternative to gene delivery, which is the current standard method for expression of specific proteins in cells. (rpi.edu)
  • To gain insight into how this can affect nucleocytoplasmic transport, we use coarse-grained molecular dynamics simulations to study the molecular interaction of poly-PR, the most toxic DPR, with several Kapβs (importins and exportins). (rug.nl)
  • Simulations estimating protein-membrane interaction strength for PGZL1 variants along an inferred maturation pathway show bilayer affinity is evolved and correlates with neutralization potency. (elifesciences.org)
  • The protein encoded by this gene appears to be multifunctional. (nih.gov)
  • Pathological Tau transmission initiated by binding lymphocyte-activation gene 3. (neurotree.org)
  • In order to approach these questions, we have used RNA-binding proteins such as ELAV/Hu, poly (A) binding protein (PABP) and cap-binding protein (eIF-4E) as reporters of gene expression. (biomedcentral.com)
  • RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors. (biomedcentral.com)
  • Our lab has devised biochemical and immunological approaches to gene expression profiling by using RNA-binding proteins as reporters of discrete mRNA subsets in metazoan cells [ 8 - 10 ]. (biomedcentral.com)
  • While we and other labs have demonstrated the isolation of mRNA subsets that are potentially co-regulated using RNA binding proteins as reporters of gene expression, methods have not been described that provide information about coordinated posttranscriptional regulation within specific types of cells during tumorigenesis and development. (biomedcentral.com)
  • Aravind L & Koonin EV 1999 Gleaning non-trivial structural, functional and evolutionary information about proteins by iterative database searches. (jmolbiochem.com)
  • Small molecules readily penetrate tissue preparations and bind their targets with known stoichiometry. (nature.com)
  • In this study, we identified the cell adhesion molecules binding to PAMPS gels that act as mechanoreceptors. (cheric.org)
  • Actin molecules in the crystal are organized into ribbons through intermolecular contacts like those found in oligomeric protein assemblies. (rcsb.org)
  • Usually, the NOE and NMRD in B-DNA is expected to be less ``integral'' than methods can only provide bounds on the residence water molecules buried inside proteins. (lu.se)
  • Poly(A)-binding protein (PAB or PABP) is an RNA-binding protein which triggers the binding of eukaryotic initiation factor 4 complex (eIF4G) directly to the poly(A) tail of mRNA which is 200-250 nucleotides long. (wikipedia.org)
  • This domain does not contact RNA, and instead, it recognizes 15 residues sequences that are a part of the PABP interaction motif (PAM-2) found on such proteins as eukaryotic translation termination factor (eRF3) and PABP interacting proteins 1 and 2 (PAIP 1, PAIP2). (wikipedia.org)
  • Here we demonstrate that the epitope-tagged RNA binding protein, PABP, expressed separately in tumor cells and endothelial cells can be used to discriminate their respective mRNA targets from mixtures of these cells without significant mRNA reassortment or exchange. (biomedcentral.com)
  • In this study, we define a model system for using poly (A) binding protein (PABP) to recover mRNAs from specific cell-types in mixed cell cultures. (biomedcentral.com)
  • Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes. (nature.com)
  • RFX forms cooperative DNA binding complexes with x2bp and cbf/nf-y. (lu.se)
  • Photophysics of green and red fluorescent proteins: Implications for quantitative microscopy. (mpg.de)
  • Poly(A) binding protein (PABPC1) normally stimulates mRNA translation by interacting with several translation initiation factors bound to the 5'- end of mRNAs. (expasy.org)
  • Most of these transcriptomic studies monitor the steady state levels of expressed mRNAs in order to derive the "molecular signatures" of tumors [ 2 ]. (biomedcentral.com)
  • For example, we identified subpopulations of mRNAs that are associated with ELAV/Hu RNA-binding proteins that are expressed in specific cell types [ 10 ]. (biomedcentral.com)
  • however, the molecular mechanism of hydrogel-dependent control of cell behavior remains unclear. (cheric.org)
  • We address the feasibility and determinant molecular features of this mechanism using integrative modeling. (elifesciences.org)
  • Glutamine-expanded Ataxin-1 causes disease by a gain-of-function mechanism given that mice lacking this protein do not reproduce SCA1 features. (bcm.edu)
  • The cytosolic isoform of eukaryotic poly(A)-binding protein binds to the initiation factor eIF4G via its C-terminal domain. (wikipedia.org)
  • eIF4G is a component of the eIF4F complex, containing eIF4E, another initiation factor bound to the 5' cap on the 5' end of mRNA. (wikipedia.org)
  • Poly(A)-binding proteins in the cytosol compete for the eIF4G binding sites. (wikipedia.org)
  • This is the location where NSP3 competes with poly(A)-binding protein for eIF4G binding. (wikipedia.org)
  • The interaction between PABPC1 and eIF4G forms a protein bridge between 5'- and 3'-ends, enhancing cap binding by eIF4E and RNA binding by eIF4G and PABPC1. (expasy.org)
  • Hassa, P.O. & Hottiger, M.O. The diverse biological roles of mammalian PARPS, a small but powerful family of poly-ADP-ribose polymerases. (nature.com)
  • The 3D model has been structurally optimised via molecular dynamics. (jmolbiochem.com)
  • The structure of human poly(A)-binding protein found in the nucleus (PABPN1) has yet to be well determined but it has been shown to contain a single RRM domain and an arginine rich carboxy terminal domain. (wikipedia.org)
  • In this work, the solubility of a fully glycosylated and a non-glycosylated human mAb expressed in corn was studied by inducing their precipitation by poly(ethylene) glycol (PEG). (mst.edu)
  • With help of the Molecularium - a spaceship that can shrink to nanoscale sizes - the group explores the "secret worlds" and molecular structures of everyday objects including a snowflake, chewing gum, a penny, and even a human cell. (rpi.edu)
  • PCBP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-163) and having a molecular mass of 19.6 kDa. (prospecbio.com)
  • Importantly, molecular alterations often swing into opposite directions in a cell- and subcellular compartment-specific manner. (nature.com)
  • Poly(A)-binding protein has also been shown to interact with a termination factor (eRF3). (wikipedia.org)
  • This model predicts that most proteins that interact with wild-type Ataxin-1 should interact with the mutant protein and that modifications of Ataxin-1 that might alter its conformation are critical to pathogenesis. (bcm.edu)
  • We found that 14-3-3 proteins interact with Ataxin-1 in a S776-dependent manner and that 14-3-3 augments levels of Ataxin-1 when phosphorylated at S776 by Akt kinase. (bcm.edu)
  • Western blot analysis indicated differential binding of integrin (ITG) isoforms to the PAMPS gel, in which the alpha 4 isoform, but not alpha 5 and alpha v, efficiently bound to the PAMPS gel. (cheric.org)
  • Rotavirus RNA-binding protein NSP3 interacts with eIF4GI and evicts the poly(A) binding protein from eIF4F. (wikipedia.org)
  • This led us to propose that wild-type Ataxin-1 might take on a conformation that resists clearance or interacts strongly with other proteins and that such conformation is favored by the expanded polyglutamine tract. (bcm.edu)
  • Current research in the Grumet lab focuses on fruit development and disease resistance in Cucumis (cucumber and melon) crops using a combination of molecular genetic, genomic and transgenic approaches. (msu.edu)
  • Here, we introduce PharmacoSTORM super-resolution imaging that combines the complementary advantages of these approaches and enables cell-type- and compartment-specific nanoscale molecular measurements. (nature.com)
  • Because the neurobiological substrate of cariprazine has remained elusive, we took advantage of PharmacoSTORM to provide in vivo evidence that cariprazine predominantly binds to D 3 dopamine receptors on Islands of Calleja granule cell axons but avoids dopaminergic terminals. (nature.com)
  • Figure 1: Macrodomain-containing proteins recruit to sites of PARP1 activity in vivo . (nature.com)
  • In this scenario, the identification of molecular biomarkers of response and the use of a multimodal in vivo imaging approach represent unique tools for better understanding tumor features, with the final goal of identifying a cluster of patients that are potential responders to personalized medicine. (mdpi.com)
  • In our group we have developed methods to quantify poly P, to stain and localize poly P and to screen for poly P binding proteins. (openwetware.org)
  • Stimulated by the pioneering studies on the cationic porphyrin TMPyP4 and the natural product telomestatin, follow-up studies have developed, rapidly leading to a large diversity of macrocyclic structures with remarkable-quadruplex binding properties and biological activities. (hindawi.com)
  • Laskowski RA, Rullmannn JA, MacArthur MW, Kaptein R & Thornton JM 1996 AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. (jmolbiochem.com)
  • In vitro studies have shown the binding affinities to be on the order of 2-7nM, while affinity for poly(U), poly(G), and poly(C) were reportedly lower or undetectable in comparison. (wikipedia.org)
  • Virtually all spirochetes in the midgut of an unfed nymph express outer surface protein (Osp) A. This protein is also the predominant surface antigen expressed by the spirochetes in vitro. (cdc.gov)
  • Chemical PARP1 inhibitors, PARP1 knockdown and mutation of ADP-ribose-binding residues in macroH2A1.1 abrogate macrodomain recruitment. (nature.com)
  • In collaboration with Harry Orr's group (University of Minnesota), we determined that the mutation responsible for SCA1 is an expansion of a CAG trinucleotide repeat encoding glutamine in the protein Ataxin-1. (bcm.edu)
  • This led us to propose that protein misfolding or impaired protein clearance might be critical in SCA1 pathogenesis. (bcm.edu)
  • The polyadenylate RNA adopts an extended conformation running the length of the molecular trough. (wikipedia.org)
  • Poly (Ethylene) Glycol (PEG) Precipitation of Glycosylated and Non-Gly" by Ke Li, Ondrea Bermudez et al. (mst.edu)
  • In this review we summarize the current state of the art in detailing the three main categories of quadruplex-binding macrocycles described so far (telomestatin-like polyheteroarenes, porphyrins and derivatives, polyammonium cyclophanes), and in addressing both synthetic issues and biological aspects. (hindawi.com)
  • It occurs ubiquitously in all living cells and regulates many molecular and biological processes. (openwetware.org)
  • Conversely, propensity to bind lipids or cell membranes poses a significant auto-immunity risk. (elifesciences.org)
  • Recent studies proposed a role for glucose-regulated protein 78 (GRP78), a protein implicated in both obesity and metabolic syndrome, which may function as a binding partner and/or co-receptor for SARS-CoV-2. (bvsalud.org)
  • The structure of the macroH2A1.1 macrodomain in complex with ADP-ribose establishes a poly-ADP-ribose cap-binding function and reveals conformational changes in the macrodomain upon ligand binding. (nature.com)
  • Deciphering the molecular organization of signaling processes and their disorganization in disease remain among the greatest challenges in biomedical science. (nature.com)