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  • proteins
  • These binding sites have a cytosolic/nuclear localization and contain the heat shock proteins hsp70 and hsp90 as components of a high molecular weight cytosolic binding complex. (pnas.org)
  • For this technique, proteins are run across an immobile gel with a pH gradient until they stop at the point where their net charge is neutral. (wikipedia.org)
  • When a vessel (typically a tube or bottle) containing a mixture of proteins or other particulate matter, such as bacterial cells, is rotated at high speeds, the inertia of each particle yields a force in the direction of the particles velocity that is proportional to its mass. (wikipedia.org)
  • While proteomics is largely a discovery-based approach that is followed by other molecular or analytical techniques to provide a full picture of the subject system, it is not limited to simple cataloging of proteins present in a sample. (wikipedia.org)
  • compounds
  • This pioneer interface for LC-MS had the same analysis capabilities of GC-MS and was limited to rather volatile analytes and non-polar compounds with low molecular mass (below 400 Da). (wikipedia.org)
  • From these earliest studies and screening numerous compounds, it was determined that for thermoreversible gels based on the amide-carbonyl hydrogen bond, amino acid structure, enantiopurity, hydrophilic-lypophilic ratio, and increasing peptide substitution greatly affected the gelling ability of various new compounds. (wikipedia.org)
  • solvent
  • Other desirable properties of the gel forming agent are the absence of ionizing groups and, in a given solvent, low affinity for the substances to be separated. (wikipedia.org)
  • All other previously known gel phase transitions have occurred as the result of temperature changes and only one previous case documents this type of solvent dependent morphological change. (wikipedia.org)
  • affinity
  • Two forms of gamma-glutamyltransferase from human brain cortex microvessels were partially purified by gel permeation and ion-exchange and group-affinity chromatography. (springer.com)
  • high
  • It is a major component of the extracellular matrix (ECM) and normally present as high molecular mass (several thousand kDa) in mammalian bone marrow and loose connective tissues ( Laurent and Fraser, 1992 ). (biologists.org)
  • The binding sites are predominantly cytosolic/nuclear and the cytosolic sites occur in a protein complex of high apparent molecular mass, ≈ 650 kDa ( 10 ). (pnas.org)
  • For gel-type ion exchangers, selectivity reversal at very high ionic strength can also provide a solution. (wikipedia.org)
  • gelation
  • The Ca/W-treatment promoted an increased rate of gelation and gave higher gel strength than the Ca/Et-treatment. (go.jp)
  • The propensity of a low molecular weight molecule to form LMOGs is classified by its Minimum Gelation Concentration (MGC). (wikipedia.org)
  • al were suitable for forming hard gels, including gels with chloroform, which had been resistant to gelation prior to their discovery. (wikipedia.org)
  • biology
  • Cell biology - study of the cell as a complete unit, and the molecular and chemical interactions that occur within a living cell. (wikipedia.org)
  • Bacteriology - study of bacteria Mycology - study of fungi Virology - study of viruses Molecular biology - study of biology and biological functions at the molecular level, with some cross over from biochemistry. (wikipedia.org)
  • Oligonucleotides find a variety of applications in molecular biology and medicine. (wikipedia.org)
  • size
  • The most common detectors used for size exclusion chromatography include online methods similar to the bench methods used above. (wikipedia.org)
  • These sample components will change levels and speed based on their size/mass. (wikipedia.org)
  • interactions
  • Gels self-assemble through non-covalent interactions such as π-stacking, hydrogen-bonding, or Van der Waals interactions to form volume-filling 3D networks. (wikipedia.org)
  • It is necessary to have the presence of strong self-complementary and unidirectional inter-molecular interactions that can enforce 1D self-assembly. (wikipedia.org)
  • amino
  • This gene is with an open reading frame of 2079 base pairs (bp), encoding a protein of 693 amino acids, corresponding to a molecular mass of 79569 Da. (wikipedia.org)
  • properties
  • Indeed EPSs produced by microorganisms from extreme habitats show biotechnological promise ranging from pharmaceutical industries, for their immunomodulatory and antiviral effects, bone regeneration and cicatrizing capacity, to food-processing industries for their peculiar gelling and thickening properties. (mdpi.com)
  • known
  • Little is known about the molecular features that govern HAstV particle assembly, maturation, infectivity, and immunogenicity. (asm.org)
  • Despite the prevalence of astroviruses, little is known at the molecular level about how the astrovirus particle assembles and is converted into an infectious, mature virus. (asm.org)
  • form
  • The MGC is the lowest possible gelator concentration needed to form a stable gel. (wikipedia.org)
  • A lower MGC is desired to minimize the amount of gelator material needed to form gels. (wikipedia.org)
  • In any form of chromatography, the rate at which the solute moves down the column is a direct reflection of the percentage of time the solute spends in the mobile phase. (wikipedia.org)
  • commonly
  • They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes. (wikipedia.org)
  • applications
  • Together, these data provide new molecular insights into HAstV that have practical applications for the development of vaccines and antiviral therapies. (asm.org)
  • Displacement chromatography found a variety of applications including isolation of transuranic elements and biochemical entities. (wikipedia.org)
  • Volume
  • The use of low dispersity standards allows the user to correlate retention time with molecular mass, although the actual correlation is with the Hydrodynamic volume. (wikipedia.org)
  • level
  • Neuroproteomics has the difficult task of defining on a molecular level the pathways of consciousness, senses, and self. (wikipedia.org)
  • typically
  • It is an example of a sol-gel process wherein a molecular precursor (typically tetraethylorthosilicate) is first reacted with water in an alcoholic solution, the resulting molecules then joining together to build larger structures. (wikipedia.org)
  • proteomics
  • By the late 20th century, new fields like genomics and proteomics were reversing this trend, with organismal biologists using molecular techniques, and molecular and cell biologists investigating the interplay between genes and the environment, as well as the genetics of natural populations of organisms. (wikipedia.org)
  • materials
  • The Stöber process is a sol-gel approach to preparing monodisperse (uniform) spherical silica (SiO 2) materials that was developed by a team led by Werner Stöber and reported in 1968. (wikipedia.org)
  • In many engineering domains dealing with mass transfer in porous materials, such as hydrogeology or heterogeneous catalysis, the tortuosity refers to the ratio of the diffusivity in the free space to the diffusivity in the porous medium (analogous to arc-chord ratio of path). (wikipedia.org)
  • results
  • Its necessity arises from the fact that - apart from relatively recent results concerning the hydrogen molecular ion (see references therein for more details) - the quantum many-body problem cannot be solved analytically, much less in closed form. (pearltrees.com)