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  • strands
  • One may make ones nucleic acid single-stranded for the purpose of annealing - if it is not single-stranded already, like most RNA viruses - by heating it in 0.01M NaCl to a point above the 'melting temperature' of the double- or partially-double-stranded form, and then flash-cooling to + 0oC: this ensures the 'denatured' or separated strands do not re-anneal. (uct.ac.za)
  • Thermodynamic analysis of interacting nucleic acid strands R.M. Dirks, J.S. Bois, J.M. Schaeffer, E. Winfree, and N.A. Pierce SIAM Rev 49(1):65-88, 2007. (wikipedia.org)
  • DNA computing requires that the self-assembly of the oligonucleotide strands happen in such a way that hybridization should occur in a manner compatible with the goals of computation. (wikipedia.org)
  • 1991
  • The OLIGO has been reviewed several times in scientific journals, for the first time in 1991 in a review in Critical Reviews in Biochemistry and Molecular Biology, and for its next upgrades (with OLIGO 7 being the latest one in 2011). (wikipedia.org)
  • NUPACK
  • Dirks wrote the initial code for the NUPACK suite of nucleic acid design and analysis tools, which generates base pairing probabilities through calculation of the statistical partition function. (wikipedia.org)
  • Dirks
  • Dirks was the first graduate student in Niles Pierce's research group at the California Institute of Technology, where his dissertation work was on algorithms and computational tools to analyze nucleic acid thermodynamics and predict their structure. (wikipedia.org)
  • nylon membranes
  • nylon membranes (Hybond-N, GeneScreen) bind all nucleic acids under a wide range of salt concentrations, and irreversible or covalent attachment can be achieved by UV irradiation for 5 min or less, or by treatment with 0.4M NaOH. (uct.ac.za)
  • Chem
  • A synthetic DNA walker for molecular transport J.-S. Shin and N.A. Pierce J Am Chem Soc 126:10834-10835, 2004. (wikipedia.org)
  • enzymes
  • He investigated how cells form enzymes, DNA and RNA structures, virology and the molecular basis of cancer. (wikipedia.org)
  • In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. (wikipedia.org)
  • Method
  • 2. The method of claim 1 , wherein a hybridization pattern showing a gain in one or more chromosome regions selected from the group consisting of: lq, 2p, 3q, 8q, l Oq, and 20q is indicative of endometrial carcinoma. (google.es)
  • 5. The method of claim 1 , wherein a hybridization pattern showing a gain in 1 q31 -qtel is indicative of endometrial carcinoma. (google.es)
  • 6. The method of claim 1, wherein a hybridization pattern showing a loss in one or more chromosome regions selected from the group consisting of: 9p, 9q, 15q, 16q, 17p, 18q, 19p, and 22q is indicative of endometrial carcinoma. (google.es)
  • amino acid
  • Secondary structure focuses on minor conformations that develop as a result of the hydrogen bonding between the amino acid chain. (wikipedia.org)
  • hybrids
  • For example, all double-stranded nucleic acids - whether dsDNA, dsRNA or RNA:DNA hybrids - have specific 'melting temperatures', which depend mainly upon their specific guanine+cytosine content, but also upon whether they are DNA, RNA, or a mixture (RNA:RNA hybrids have the highest melting temperatures, followed by DNA:RNA hybrids, then dsDNA), and upon the ionic strength of solution. (uct.ac.za)
  • involves
  • The duplex formed between the nucleic acid and the loop is more stable than that of the stem because the former duplex involves more base pairs. (wikipedia.org)