Molecular human granulocyte macrophage colony stimulating factor. Medical search. Frequent questions

GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. (wikipedia.org)
Monocytes exit the circulation and migrate into tissue, whereupon they mature into macrophages and dendritic cells. (wikipedia.org)
GM-CSF stimulates monocytes and macrophages to produce pro-inflammatory cytokines, including CCL17. (wikipedia.org)
Although tumor cells were first thought to drive the cellular events underpinning tumor angiogenesis and growth, considerable evidence has now emerged for the central role of tumor infiltrating myeloid cells such as monocytes, macrophages, and neutrophils in this phenomenon [ 8 - 12 ]. (hindawi.com)
monocytes derived dendritic cells have been widely used as a model system to explore the molecular mechanisms of human DCs differentiation. (biotechnologyconsultinggroup.com)
Previous studies showed that granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL4 drive peripheral blood CD14+ monocytes differentiation to CD14?CD1a+ DCs (2, 9). (biotechnologyconsultinggroup.com)
To overcome this limitation, we have recently developed a technique to create human-induced microglia-like (iMG) cells from human peripheral blood monocytes. (nature.com)
Immunomagnetically selected peripheral blood monocytes are cultured in RPMI 1640 Medium (Catalog #36750) + 10% FBS, M-CSF, and IL-4 for 5 days to generate macrophages. (stemcell.com)
CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. (thermofisher.com)
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. (thermofisher.com)
Kupffer cells, hepatic stellate cells and sinusoidal endothelium) and infiltrating leukocytes (e.g., monocytes, monocyte-derived macrophages, neutrophils and lymphocytes). (mdpi.com)
Tissues differentially secrete multiple colony stimulating factors under conditions of homeostasis and inflammation, orientating recruited circulating monocytes to differentiate to macrophage with differing functional phenotypes. (uea.ac.uk)

The innate immune system is mainly composed of myeloid lineage cells, such as macrophages, neutrophils, and mast cells [ 2 ]. (hindawi.com)
Human neutrophils migrate towards Ehrlichia sp. (neuroprobe.com)
Manganese superoxide dismutase and heat shock protein 70 are not necessary for suppression of apoptosis in human peripheral blood neutrophils. (mcmaster.ca)
We have previously shown that granulocyte (G-CSF) and granulocyte/macrophage (GM-CSF) colony-stimulating factors present in human bronchial epithelial cell conditioned medium (HBEC-CM) suppress apoptosis in neutrophils. (mcmaster.ca)
It stimulates the survival, proliferation, differentiation and function of neutrophil granulocyte progenitor cells and mature neutrophils. (allindianpatents.com)
describes a novel colony stimulating factor (CSF) that has the ability to promote the differentiation and proliferation of human bone marrow cells to neutrophils, and a method to produce the same from a novel cell line which has been established from tumor cells in patients with oral cancer, The most published studies have used filgrastim as it was the first form of G-CSF to be approved. (allindianpatents.com)
Human Granulocyte-Colony-Stimulating Factor (G-CSF), a 20 kDa glycoprotein containing internal disulfide bonds, induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. (sydlabs.com)

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. (wikipedia.org)
Specific growth factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), stimulate the proliferation and migration of naturally quiescent endothelial cells, resulting in the formation of new vessel structures during embryonic development and tumor growth [ 7 ]. (hindawi.com)
Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. (biolegend.com)

Macrophages and dendritic cells can produce 1,25-dihydroxyvitamin D within the microenvironment. (springer.com)

Unlike granulocyte colony-stimulating factor, which specifically promotes neutrophil proliferation and maturation, GM-CSF affects more cell types, especially macrophages and eosinophils. (wikipedia.org)
Combined treatment with selumetinib and a dose of palbociclib sufficient to reinforce G1 arrest in selumetinib-sensitive cells, but not to impair proliferation of resistant cells, delays the emergence of resistant colonies, meaning that escape from G1 arrest is critical in the formation of resistant clones. (babraham.ac.uk)

Granulocyte colony stimulating factor (G-CSF) is a 20 kDa glycoprotein cytokine that stimulates growth and development of granulocyte progenitors in the bone marrow (Nagata et al. (allindianpatents.com)
As a first step toward the goal of defining the effects of DP treatment on haematopoiesis of preterm infants, we designed this in vitro study to determine whether DP affects the clonal growth of erythroid or granulocyte-macrophage progenitor cells in preterm infants, and if the preterm infant's progenitors respond to DP in a different manner from those of adults. (bmj.com)
The effects of DP on the clonal growth of erythroid and granulocyte-macrophage progenitors were investigated using clonogenic assays according to procedures previously tested in our laboratory. (bmj.com)
The concentrations of erythropoietin (Epo) used to promote the growth of erythroid colonies ranged from 0.2 U/ml to 20 U/ml, based on the possibility that very high Epo concentrations might mask the effects of DP on progenitors of preterm infants. (bmj.com)
In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors. (biolegend.com)

GM-CSF is a monomeric glycoprotein that functions as a cytokine-it is a white blood cell growth factor. (wikipedia.org)
The cytokine activates macrophages to inhibit fungal survival. (wikipedia.org)
The human gene has been localized in close proximity to the interleukin 3 gene within a T helper type 2-associated cytokine gene cluster at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. (wikipedia.org)
Vitamin D inhibits monocyte/macrophage proinflammatory cytokine production by targeting MAPK phosphatase-1. (springer.com)
Resveratrol, but not estradiol or N-acetyl cysteine, inhibited cytokine-stimulated inducible nitric oxide synthase expression and nitrite production (IC50 = 3.6 +/- 2.9 microM) in human primary airway epithelial cells. (lacienciadelvino.com)

SM can trigger several molecular pathways involved in inflammation and oxidative stress, which cause cell necrosis and apoptosis, and loss of cells integrity and function. (ac.ir)
Evaluation of the sensitization phase as an endpoint stimulate keratinocytes present in the epidermis of the skin results in a reduced time for animals to be on study and to release inflammatory mediators such as interleukins 1, 6 eliminates the discomfort associated with the development of and 18, granulocyte-macrophage colony-stimulating factor, inflammation in the elicitation phase of the response. (cdc.gov)

A 32P-postlabeling method for the detection of adducts in the DNA of human fibroblasts exposed to sulfur mustard. (ac.ir)

Members of the transforming growth factor beta (TGFβ) family also contribute to inhibition of osteoblast differentiation by metastatic breast cancer cells [ 6 , 7 ]. (biomedcentral.com)
Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. (sydlabs.com)

G-/GMCSF activates the JAK family of receptor tyrosine kinases, which unfolds its activity by not only regulating enzymes and target proteins within its local milieu, but importantly also by activating the STAT family of transcription factors, which subsequently dimerize and translocate to the cell nucleus to regulate gene expression[ 13 ]. (biomedcentral.com)
Low affinity receptor for granulocyte-macrophage colony-stimulating factor. (cusabio.com)
Conformational changes in the GM-CSF receptor suggest a molecular mechanism for affinity conversion and receptor signaling. (cusabio.com)
Finally, immunocompetent and severe combined immunodeficient mice administered CXCR2 antisera, and CXCR2 −/− mice that lack the human IL-8 receptor homologue, are much less susceptible to granulocytic ehrlichiosis than are control animals. (neuroprobe.com)
Currently, the clinical interventions for patients with BC-bone metastasis (BC-BM) are bisphosphonates (BPs) or denosumab (an anti-receptor activator of nuclear factor kappa B ligand (RANKL) monoclonal antibody), which disrupt the vicious cycle by targeting osteoclasts [ 6 ]. (biomedcentral.com)
These include defects in genes encoding for surfactan protein B or C, transporter molecules within type II pneumocytes, and the beta chain receptor of granulocyte-macrophage colony-stimulating factor (GM-CSF). (medscape.com)
This study reveals the contribution of P2X4 and P2Y11 receptor activation to ATP-evoked intracellular Ca2+ responses, and makes comparison with macrophage differentiated using granulocyte colony-stimulating factor (GM-CSF). (uea.ac.uk)

Microglia are immune cells in the CNS, and known to have inflammatory functions via releasing proinflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-1β 6 . (nature.com)
Special considerations are given to the causal involvement of novel molecular pathways, including cytokines, growth factors, and intracellular signaling cascades in RA pathophysiology, and the emerging role of targeted therapeutic agents in the management of RA. (jscimedcentral.com)
Out of the 313 unique human cytokines evaluated, 11 were found to expand cell numbers ≥2-fold in a 7-day culture. (haematologica.org)

The important function of VentX in hematopoietic cells advancement was indicated by its function in managing the growth and difference of Compact disc34+ cells and monocyte to macrophage fatal difference (16, 17). (biotechnologyconsultinggroup.com)

Serum amyloid A and alpha-1 acid glycoprotein are transport proteins, and fibrinogen is a coagulation factor. (msdmanuals.com)

13 ] isolated mononuclear cells from human peripheral blood that were enriched for expression of the hematopoietic stem cell marker CD34 [ 13 ]. (hindawi.com)
Recent studies indicate that some hematopoietic growth factors, namely granulocyte macrophage colony stimulating factor (GMCSF) and granulocyte colony stimulating factor (GCSF), are abundantly released in the tumor microenvironment and play a key role in regulating tumor-nerve interactions and tumor-associated pain by activating receptors on dorsal root ganglion (DRG) neurons. (biomedcentral.com)
Moreover, these hematopoietic factors have been highly implicated in postsurgical pain, inflammatory pain and osteoarthritic pain. (biomedcentral.com)
Several types of non-hematopoietic tumors secrete hematopoietic colony stimulating factors, which act on myeloid cells and tumor cells[ 7 ]. (biomedcentral.com)

Concentrations of 10 6 M to 10 9 M were associated with a dose dependent inhibition of colony formation, with the most clinically important effects seen on the earliest erythroid and granulocyte-macrophage colonies. (bmj.com)
For erythroid colony forming unit (CFU-E) quantification, we used 2000 cells/ml, while for erythroid burst forming units (BFU-E), colony forming units mix (CFU-mix), and granulocyte-macrophage colony-forming unit's (CFU-GM), we used 7000 cells/ml. (bmj.com)
In this study DP inhibited, in a dose dependent manner, the numbers of both immature and more mature erythroid colonies (figs 1-3 ). (bmj.com)

1 In humans, glucocorticoid receptors have been shown in many different cells, including those of the granulocyte-macrophage lineage and lympho cytes. (bmj.com)
Binding studies with 125I-labeled recombinant human (rh) IL-3 indicated that IL- 3 bound to a single class of high affinity receptors (approximately 4,000 receptors/cell) on KMT-2 cells with a kd of approximately 200 pmol/L. The chemical cross-linking assay demonstrated that radiolabeled hIL-3 bound three molecules with molecular masses of 170, 130, and 70 Kd. (ashpublications.org)
Present data suggest that the newly established human cell line will be a valuable tool for the biologic assay of hIL-3, and a model for biochemical studies of IL-3 receptors. (ashpublications.org)

EP0237545, published on 1987-09-23 by Lawrence M Souza discloses the production of pluripotent granulocyte colony stimulating factor using E.coli as a host organism. (allindianpatents.com)

Subsequent studies described a VEGFR2 and AC133 expressing subpopulation of these CD34 positive circulating cells that could form endothelial colonies in vitro [ 14 , 15 ]. (hindawi.com)
In this report, we examined the mode of nitrite reductase (PNR) release from a serum-sensitive strain of live P. aeruginosa cells during in vitro treatment with four different antimicrobial agents or human complement. (neuroprobe.com)
Furthermore, the culture supernatants of the BET-1A cells stimulated with aliquots of bacterial culture containing antimicrobial agents or complement similarly mediated neutrophil migration in vitro. (neuroprobe.com)
outlined only ten TBI-related published proteomic studies, five of which were conducted in human samples (TBI, AD or CTE), two in in-vitro models and three in in-vivo animal models [ 10 ]. (biomedcentral.com)
Hel-N1 has been shown to bind in vitro to 3' untranslated regions of mRNAs encoding c-myc, c-fos, granulocyte/macrophage colony-stimulating factor, and transcriptional repressor, Id. (duke.edu)

Myeloid cells promote tumor growth by stimulating tumor angiogenesis, suppressing tumor immunity, and promoting metastasis to distinct sites. (hindawi.com)

As shown for the Macrophage Inflammatory Protein 1α, de novo RNA synthesis correlates with the number of CpG dinucleotides, whereas RNA splicing, stability, nuclear export and translation are not affected by the sequence modification. (nih.gov)
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)
The expression of tumor necrosis factor (TNF)-α at mRNA and protein levels significantly increased in ATP-stimulated iMG cells from patients with fibromyalgia compared to cells from healthy individuals. (nature.com)
For this purpose, WIN 55,212-2 was injected in pregnant wistar rats from gestation day 5 to 20 and a detailed analysis of the levels of the neurotrophin brain-derived neurotrophic factor (BDNF) as well as of the signaling molecules extracellular signal-regulated kinase (ERK)1/2 and alpha-calcium/calmodulin-dependent protein kinase II (alpha-CaMKII) was carried out in adult offspring. (researchgate.net)
The present invention relates to a chimeric protein of recombinant human granulocyte colony stimulating factor comprising of hG-CSF fused to an affinity fusion tag at its amino terminus. (allindianpatents.com)
One way to produce recombinant human G-CSF in soluble form is to produce chimeric protein formed by expression of a hybrid gene made by splicing together two gene sequences, In this approach, ideally GCSF would be operationaffy finked to an affinity tag so as to give chimeric protein with better solubility. (allindianpatents.com)
Hel-N1, a human RNA-binding protein, shares significant homology with Drosophila protein ELAV, which is essential for fly neuronal development. (duke.edu)
There are two forms, 174- and 180-amino-acid, of the natural human G-CSF protein. (sydlabs.com)
The structure of the recombinant human G-CSF protein expressed in E. coli, such as filgrastim, differs slightly from those of the natural glycoprotein and the recombinant human G-CSF protein expressed in mammalian cells such as CHO cells. (sydlabs.com)
PEGylation (polyethylene glycol) significantly extends half-life of the recombinant human G-CSF protein. (sydlabs.com)
Low molecular weight (LMW) assay (LLNA) was developed in 1989 [2] and continues to chemical sensitizers, referred to as haptens (or prohaptens), undergo refinement as an alternative for the evaluation of are themselves too small to be allergenic and must bind to sensitizing potential of low molecular weight (LMW) chem- a protein to be allergenic. (cdc.gov)

Amongst these, we validate calpain 2, matrix metalloproteinase 9 (MMP9) and a RhoGTPase Rac1 as well as Tumor necrosis factor alpha (TNFα) as transcriptional targets of G-/GMCSF and demonstrate the importance of MMP9 and Rac1 in GMCSF-induced nociceptor sensitization. (biomedcentral.com)

Recombinant human G-CSF is a potent stimulant of granulopoiesis and is useful in preventing infectious complications of some neutropenic states. (allindianpatents.com)
The recombinant human G-CSF synthesised in an E. coli expression system is called filgrastim. (allindianpatents.com)
Another form of recombinant human G-CSF called lenograstim is synthesised in Chinese hamster ovary (CHO) cells. (allindianpatents.com)
A novel human cell line, KMT-2, from umbilical cord blood cells was established based on the selection of cultures in the presence of recombinant human interleukin-3 (IL-3) and the sorting of cells with anti-My 10 antibody. (ashpublications.org)

1 Department of Molecular Microbiology & Gene Therapy, Institute of Medical Microbiology and Hygiene, University Hospital of Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany and Department of Biochemistry III, Institute for Biochemistry, Genetics and Microbiology, University of Regensburg, Universitätsstr. (nih.gov)

Anti-inflammatory effects of resveratrol in lung epithelial cells: molecular mechanisms. (lacienciadelvino.com)
This study examined the anti-inflammatory effects of resveratrol on human airway epithelial cells. (lacienciadelvino.com)
Bacterial killing of P. aeruginosa by antimicrobial agents induced PNR release and mediated IL-8 production in human bronchial epithelial (BET-1A) cells. (neuroprobe.com)

Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. (thermofisher.com)
Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. (thermofisher.com)

The molecular cloning of GCSF gene (Nagata et al. (allindianpatents.com)

Here, we investigated ATP-evoked intracellular Ca2+ responses in human macrophage differentiated with macrophage colony-stimulating factor (M-CSF). (uea.ac.uk)

Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. (elsevierpure.com)

The natural human glycoprotein exists in two forms of 174 and 177 amino acids. (allindianpatents.com)

LC3-II-axis impacts on fungal immunity in human macrophages. (uc.edu)
Tumor Necrosis Factor Alpha Antagonism Reveals a Gut/Lung Axis That Amplifies Regulatory T Cells in a Pulmonary Fungal Infection. (uc.edu)

These observations stimulated an intensive search for the mechanisms regulating tumor angiogenesis. (hindawi.com)
However, the molecular mechanisms via which G-/GMCSF bring about nociceptive sensitization and elicit pain are not known. (biomedcentral.com)
Dankers W, Colin EM, van Hamburg JP, Lubberts E. Vitamin D in autoimmunity: molecular mechanisms and therapeutic potential. (springer.com)

The article also considers the impact of genetic variations on RA therapeutics in the context of molecular medicine and individualized patient care. (jscimedcentral.com)
1976. Combination of genetic factors and chronic exposure to benzene in the aetiology of leukaemia. (cdc.gov)

The Runt-related transcription factor 2, Runx2, is an important determinant of bone metastasis in breast cancer. (biomedcentral.com)

however, mRNAs encoding a subset of factors important for error-free replication and chromosome segregation, including TIPIN, PLK2 and PLK3, remain at low abundance. (babraham.ac.uk)

We have previously demonstrated that Runx2 requires the co-activator core binding factor beta (CBFβ) to regulate gene expression in breast cancer cells. (biomedcentral.com)

HPS occurs primarily in the fall, when small rodents (eg, field mice) inhabit human dwellings to protect themselves from the cold weather. (medscape.com)

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714-82) (blue histogram) or Anti-Human CD14 Super Bright 645 (purple histogram). (thermofisher.com)

The human immune system is composed of an innate and an adaptive branch. (hindawi.com)
Kamen DL, Tangpricha V. Vitamin D and molecular actions on the immune system: modulation of innate and autoimmunity. (springer.com)
NSCLC without driver mutations is one of the most heavily mutated of human cancers [ 8 ], thus it is likely that NSCLC cells harbor neoantigens to which the host would not be tolerant, and against which the immune system could mount a strong, destructive immune response. (biomedcentral.com)
Cellular Components of the Immune System The immune system consists of cellular components and molecular components that work together to destroy antigens. (msdmanuals.com)

Lung cellular and molecular physiology (Am J Physiol Lung Cell Mol Physiol. (lacienciadelvino.com)

Modulation of the Human Pancreatic Ductal Adenocarcinoma Immune Microenvironment by Stereotactic Body Radiotherapy. (rochester.edu)

These rodent studies have indicated the over-activation of microglial cells in patients with chronic pain, however clinical molecular data are lacking due to ethical and technical issues. (nature.com)

The aim of our study was to identify molecular changes produced by prenatal exposure to WIN 55,212-2 that might contribute to late disruption in synaptic plasticity and cognition. (researchgate.net)
These include various infections, hematologic malignancies, inorganic dust exposure (silica, aluminum, or titanium), and immunodeficiency states such as infection with human immunodeficiency virus ( HIV ) and lung transplantation . (medscape.com)
Molecular and cellular mechanism of lung injuries due to exposure to sulfur mustard: a review. (ac.ir)

However, the molecular mechanism of Rb rescueof the class II genes is unknown. (neuroprobe.com)
The mechanism underlying circIKBKB-mediated activation of NF-κB/bone remodeling factors signaling and EIF4A3-induced circIKBKB were investigated using RNA pull-down, luciferase reporter, chromatin isolation by RNA purification and enzyme-linked immunosorbent assays. (biomedcentral.com)

Hier sind IL-2 Antikörper für eine Vielzahl von Species wie anti-Human IL-2, anti-Mouse IL-2, anti-Rat IL-2 zu finden. (antikoerper-online.de)
Combined, the published data do not present a consistent message, owing to the diverse models of TBI under investigation, and differences in timing of assessment post-injury, species, animal genotypes, and other factors. (biomedcentral.com)
Index fungorum ( http://www.indexfungorum.org/ ) lists 17 species names under this genus, but molecular phylogenies have reduced the number to ten (Zhao et al. (biomedcentral.com)

The development of the human blood-CSF-brain barrier. (cdc.gov)

Thus, it is part of the immune/inflammatory cascade, by which activation of a small number of macrophages can rapidly lead to an increase in their numbers, a process crucial for fighting infection. (wikipedia.org)

Anatomy33

Organisms9

Diseases13

Chemicals and Drugs74

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes35

Disciplines and Occupations1

Information Science3

Monocytes12

Neutrophils7

Endothelial cells3

Dendritic cells1

Proliferation2

Progenitors5

Cytokine5

Inflammation2

Fibroblasts1

Differentiation2

Receptor7

Cytokines3

Monocyte1

Proteins1

Hematopoietic4

Erythroid3

Receptors3

Pluripotent1

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Myeloid cells1

Protein11

Transcriptional1

Recombinant human4

Genetics1

Epithelial3

Antibody3

GCSF1

Intracellular1

Spectroscopy1

Glycoprotein1

Fungal2

Mechanisms3

Genetic2

Transcription1

MRNAs1

Activator1

Mice1

Isotype1

Immune system4

Cellular and molecular1

Microenvironment1

Clinical1

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Mechanism2

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