• We assign transcription initiation sites to 7691 protein-coding genes and find that they display features typical of eukaryotic promoters. (nih.gov)
  • These heritable epigenetic modifications include DNA methylation of the promoter regions of genes that affect sensitivity to stress. (wikipedia.org)
  • Epigenetic modification in response to stress results in molecular and genetic alterations that in turn results in mis-regulated or silenced genes. (wikipedia.org)
  • Maternal separation and postnatal maternal abuse also increases DNA methylation at regulatory regions of BDNF genes in the prefrontal cortex and hippocampus, leading to potential stress vulnerability in future generations. (wikipedia.org)
  • Stress can also result in inheritable changes DNA methylation in the promoter regions of the estrogen receptor alpha (ERα), glucocorticoid receptor (GR), and mineralocorticoid receptor (MR). These changes lead to altered expression of these genes in offspring that in turn leads to decreased stress tolerance. (wikipedia.org)
  • Molecular analysis revealed that the ciD allele is caused by an inversion event that swapped the promoter regions and the first exons of the two genes. (uzh.ch)
  • Here we addressed the molecular mechanism behind repression of non-lymphoid genes in B cells. (eur.nl)
  • We found that HDAC7 specifically interacted with the transcription factor MEF2C in pre-B cells and was recruited to MEF2 binding sites located at the promoters of genes critical for macrophage function. (eur.nl)
  • In addition, HBV polymerase could inhibit the transcriptional activity of other IFN-stimulated response element-driven promoters and the expression of interferon-stimulated genes (ISGs), such as Stat1 and ISG15. (edu.au)
  • We than analyzed full-length penA and pilQ gene sequences and other genetic determinants, including mtrR , penB , and ponA genes. (cdc.gov)
  • This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
  • This can be done through genetic engineering, which involves the insertion of specific genes or DNA sequences into an organism's genome. (tutorialspoint.com)
  • One important tool in molecular analysis is the use of reporter genes, which are genes that encode for easily detectable proteins or enzymes that are used to measure gene expression levels. (tutorialspoint.com)
  • Reporter genes can be fused to the regulatory regions of other genes, allowing the expression of the target gene to be monitored in real-time. (tutorialspoint.com)
  • Another important tool in molecular analysis is the use of microarray analysis, which allows the expression of thousands of genes to be monitored simultaneously. (tutorialspoint.com)
  • Moreover, replication ensures that genetic information stays consistent and the life story written in our genes continues for another generation. (aboutdarwin.com)
  • This pathway describes progression of a serrated precursor lesion, often followed by the onset of epigenetic instability involving promoter methylation and silencing of key tumour suppressor genes, and accounts for 15%-20% of sporadic colorectal cancer [ 1 , 2 ]. (hindawi.com)
  • Identification and in silico characterization of structural and functional impacts of genetic variants in milk protein genes in the Zebu breeds Guzerat and Gyr. (fiocruz.br)
  • Whole genome sequencing of bovine breeds has allowed identification of genetic variants in milk protein genes. (fiocruz.br)
  • In a Novartis-sponsored study in the New England Journal of Medicine, researchers found that a CRISPR-Cas9-based treatment targeting promoters of genes encoding fetal hemoglobin could reduce disease symptoms. (genomeweb.com)
  • 11 genes and promoter regions. (msdmanuals.com)
  • We attempted to dissect the contribution of individual polymorphisms in this gene-dense region by linkage disequilibrium mapping across 6 adjacent genes. (ox.ac.uk)
  • Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)
  • and during this study the genetic characterisation of ESBLs genes was investigated. (who.int)
  • Context-specific functional effects of IFNGR1 promoter polymorphism. (ox.ac.uk)
  • We report evidence of a polymorphism in the promoter region of IFNGR1 (encoding interferon-gamma receptor 1) that has opposite functional effects in different cellular contexts. (ox.ac.uk)
  • furthermore, by studying different biogeographical populations, conservation of the coding region of the MRP3 gene and variability (polymorphism) in its promoter region were found (Russo et al. (szn.it)
  • Low Molecular Mass Polypeptide 7 Single Nucleotide Polymorphism is Associated with the Progression of Liver Fibrosis in Patients Infected with Hepatitis C Virus Genotype 4. (cdc.gov)
  • Both of these haplotypes contained the TNF promoter polymorphism -1031C, an allele that was associated with disease even in individuals who did not carry either HLA-B*51 or HLA-B*5701. (ox.ac.uk)
  • The rs2234762 polymorphism, sited in the NTS gene promoter, was statistically more frequent in the lowest pro-NTS level group. (lu.se)
  • We therefore studied the association of two polymorphisms - R353Q polymorphism at codon 353 involving the catalytic region and the 10 base pair (bp) insertion polymorphism involving the promoter region - with FVIIc levels in 176 healthy Tunisians. (who.int)
  • Our genetic and in situ hybridization data indicate that ciD is a mutation affecting both ci and pan. (uzh.ch)
  • Researchers at the Salk Institute have zeroed in on the mechanisms that activate oncogenes, showing that genetic mutation activity depends on the distance. (scienceboard.net)
  • This review highlights the importance of the BRAF mutation occurring in colorectal cancer stratified for molecular background and discusses its prognostic and clinical significance. (hindawi.com)
  • This latter pathway involves the previously well-defined series of genetic aberrations such as APC mutation and chromosomal instability and accounts for the majority of colorectal cancer [ 12 ]. (hindawi.com)
  • Recent studies have also found that presence of the BRAF mutation has direct implications for clinical management as along with TNM stage, BRAF mutational status was the only molecular variable that independently accounted for poor survival [ 13 ], and studies have found that BRAF mutant cancers are refractory to anti-EGRF therapy [ 14 , 15 ]. (hindawi.com)
  • The mutations associated with resistance to many of the antituberculosis drugs have been identified, though much work remains to be done to identify the molecular basis of resistance for some of the drugs and to determine the predictive value of finding a particular mutation in a strain of M. tuberculosis (1,2). (cdc.gov)
  • Molecular characterization of a novel splice site mutation within the CYBB gene leading to X-linked chronic granulomatous disease. (lu.se)
  • This disease, previously referred to as MCKD type 1, is due to a mutation in the variable-number tandem repeat region of the MUC1 (Mucin 1) gene. (medscape.com)
  • Chronic variable stress induces offspring hypothalamic gene expression modifications, including elevated methylation levels of the BDNF promoter in the hippocampus. (wikipedia.org)
  • Female offspring with low licking-grooming mothers have decreased promoter methylation and increased histone acetylation, leading to increased glucocorticoid receptor expression. (wikipedia.org)
  • Epigenetic modifications as a result of absent maternal care lead to decreased estrogen receptor alpha expression, due to increased methylation at the gene's promoter. (wikipedia.org)
  • The research suggests that the gene regulates FAAH expression by stopping DNMT1-dependent DNA methylation of the FAAH promoter. (scienceboard.net)
  • IDH1/2 mutations are the histological classification and avoids the TeT2 pRomoteR methylation in low- most significant predictor of favourable ambiguity inherent to the diagnosis of gRade diffuse gliomas lacking idh1/2 outcome of glioblastoma patients. (who.int)
  • Studies show that certain PAH metabolites interact with DNA and are genotoxic, causing malignancies and heritable genetic damage in humans. (cdc.gov)
  • To study the molecular mechanism(s) of the inhibition of MyD88 expression by HBV, MyD88 promoter reporter plasmids and vectors expressing different HBV viral proteins were constructed. (edu.au)
  • We find that the IFNGR1-470del allele acts to suppress binding of nuclear proteins to the IFNGR1 promoter region in a manner that is specific for cell type. (ox.ac.uk)
  • Genetic analysis involves the study of an organism's genetic material, including its DNA, RNA, and proteins. (tutorialspoint.com)
  • Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. (lookformedical.com)
  • These antigens include organic dusts containing bacteria, fungi,4 animal or plant proteins, or low-molecular-weight chemicals. (cdc.gov)
  • Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)
  • Mutations in the 5'-untranslated and/or coding regions were identified that may affect transcription efficiency. (manchester.ac.uk)
  • 2004 ) Genetic evidence for pre-recruitment as the mechanism of transcription activation by SoxS of Escherichia coli: the dominance of DNA binding mutations of SoxS. (academictree.org)
  • Mutations in regulatory regions or other untranslated regions are not detected by this test. (cincinnatichildrens.org)
  • Promoter analysis of PTEN will identify mutations in about 10% of patients with Cowden syndrome. (cincinnatichildrens.org)
  • For example, approximately 95% of rifampin-resistant M. tuberculosis strains carry mutations within the rifampin-resistance determining region (RRDR), an 81-bp region encoding codons 507 through 533 of the rpoB gene. (cdc.gov)
  • Molecular genetic tests for detecting drug-resistance are, in general, just a variation of nucleic acid amplification (NAA) tests and can reliably provide information on the presence of mutations associated with drug resistance in 1 to 2 days. (cdc.gov)
  • Because molecular beacons can use different fluorophores, real-time PCR assays can be designed in which different DNA fragments or mutations can be amplified and detected simultaneously in the same tube. (cdc.gov)
  • For example, a single-well assay has been developed that uses five molecular beacons to detect mutations associated with rifampin resistance in M. tuberculosis bacteria and appears to perform similarly as the line-probe assays. (cdc.gov)
  • They analyzed more than 650,000 genetic variants in both coding and non-coding regions of the human genome and found widespread patterns of how these mutations can cause splicing errors. (genomeweb.com)
  • Genetic diversity of hepatitis B virus and mutations associated to hepatocellular carcinoma in patients from Venezuela, with different stages of liver disease. (cdc.gov)
  • Over 95% of RIF-resistant M. tuberculosi s strains have mutations within the RIF resistance-determining region (RRDR) of the rpoB gene, and several molecular genetic tests to detect RRDR mutations have been developed. (msdmanuals.com)
  • Point mutations within this region have further confirmed the role of this site, along with a more minor role for a neighbouring PEA3 site, in basal expression. (uea.ac.uk)
  • mutations high frequency of IDH1/2 mutations in oligodendrogliomas, astrocytomas and in alteRations in the RB1 pathway in The TET2 gene encodes the -KG- secondary glioblastomas derived thereof low-gRade diffuse gliomas lacking dependent enzyme that catalyses suggests that these tumours share a common genetic alteRations the conversion of 5-methylcytosine to common progenitor cell population. (who.int)
  • Molecular epidemiology of chronic granulomatous disease in a series of 80 kindreds: identification of 31 novel mutations. (lu.se)
  • We purified and sequenced PCR products by using an ABI 3500 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) and performed molecular epidemiologic characterization by using N. gonorrhoeae multiantigen sequence typing ( http://www.ng-mast.net ). (cdc.gov)
  • These results provide unique insight into the GRAS family of TFs and novel regulatory targets for further molecular characterization. (usda.gov)
  • Molecular characterization demonstrates that one T-DNA insert was integrated into the cotton genome to produce cotton 1445. (cbd.int)
  • The molecular mechanisms that allow enhancers to bypass insulators are not currently well understood. (nih.gov)
  • Our findings provide insights into the molecular and genetic mechanisms underlying inflorescence development in tomato. (nature.com)
  • To gain insight into the molecular mechanisms mediating HDAC7 repression in pre-B cells, we undertook co-immunoprecipitation and chromatin immunoprecipitation experimental approaches. (eur.nl)
  • Genetic and molecular analyses are essential in understanding the mechanisms underlying transgenesis and in evaluating the potential risks and benefits associated with the use of transgenic organisms. (tutorialspoint.com)
  • Molecular analysis involves the study of the molecular mechanisms underlying gene expression and regulation in transgenic organisms. (tutorialspoint.com)
  • This includes the study of the promoter regions that regulate gene expression, the splicing mechanisms that generate different mRNA isoforms, and the translation mechanisms that produce the final protein products. (tutorialspoint.com)
  • Here, we investigated genetic and epigenetic mechanisms that affect AhR expression. (umn.edu)
  • The project aims to study the genetic controls and the molecular mechanisms involved in sex regulation in different species belonging to the genus Pseudo-nitzschia, by studying the genome and transcriptome of strains with different MT, also using genetic transformation, protein analysis and epigenetic analysis tools. (szn.it)
  • Directed elongation from an upstream enhancer toward a downstream gene could potentially deliver RNA polymerase II to a proximal promoter, or alternatively might function directly as a distal promoter. (nih.gov)
  • Co-transfection experiments showed that IFN-induced MyD88 promoter activity was inhibited by HBV polymerase expression in a dose-dependent manner and that the terminal protein (TP) domain of HBV polymerase was responsible for this antagonistic activity. (edu.au)
  • Analysis of site mutants showed that the region targeted by the polymerase protein contained the signal transducer and activator of transcription (Stat) binding site. (edu.au)
  • In summary, these results indicate that HBV polymerase is a general inhibitor of IFN signalling and can inhibit IFN-inducible MyD88 expression by inhibiting the activity of the MyD88 promoter through blocking the nuclear translocation of Stat1. (edu.au)
  • 2012 ) Transcription activation by Escherichia coli Rob at class II promoters: protein-protein interactions between Rob's N-terminal domain and the σ(70) subunit of RNA polymerase. (academictree.org)
  • 2011 ) Genetic evidence for a novel interaction between transcriptional activator SoxS and region 4 of the σ(70) subunit of RNA polymerase at class II SoxS-dependent promoters in Escherichia coli. (academictree.org)
  • 2010 ) Protein-protein interactions between sigma(70) region 4 of RNA polymerase and Escherichia coli SoxS, a transcription activator that functions by the prerecruitment mechanism: evidence for "off-DNA" and "on-DNA" interactions. (academictree.org)
  • 2004 ) Novel protein--protein interaction between Escherichia coli SoxS and the DNA binding determinant of the RNA polymerase alpha subunit: SoxS functions as a co-sigma factor and redeploys RNA polymerase from UP-element-containing promoters to SoxS-dependent promoters during oxidative stress. (academictree.org)
  • The most common method of genetic analysis is polymerase chain reaction (PCR), which is used to amplify specific DNA sequences. (tutorialspoint.com)
  • In silico analyses and mRNA half-life determinations linked insertions of nucleotide repeats 3' to the -10 motif of the norA promoter with increased promoter activity. (manchester.ac.uk)
  • The sensitivity of DNA sequencing is over 99% for the detection of nucleotide changes, small deletions and insertions in the regions analyzed. (cincinnatichildrens.org)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • Molecular beacons can discriminate between targets differing by a single nucleotide. (cdc.gov)
  • We employ a variety of experimental systems for our studies ranging from molecular biology to transgenic and knockout mice. (stanford.edu)
  • Molecular Biology and Evolution , 21 (3), 555-562. (ncsu.edu)
  • However, the related molecular biology is not easy to be understood. (aporc.org)
  • By understanding precisely what is happening at a molecular level, we can start to understand the biology involved, and that opens up possibilities for drug discovery that could one day have far-reaching positive impacts for patients. (scienceboard.net)
  • In 10 of 14 tumors investigated the breakpoints on the myc carrying chromosome were clustered in a 1.5 kb region 5' of the intact gene, proximal to the myc promoters. (lu.se)
  • Analyses of the human AHR proximal promoter in MCF-7 human breast cancer cells using luciferase assays and electrophoretic mobility shift assays revealed multiple specificity protein (Sp) 1 binding sequences that are transcriptional activators in vitro. (umn.edu)
  • Analysis of the AHR proximal promoter region using chromatin immunoprecipitation confirmed that enhanced expression of AhR in LTEE cells involves changes in histone modifications, notably decreased trimethylation of histone 3, lysine 27. (umn.edu)
  • In addition, we use bioinformatic analysis across twelve Drosophila genomes to identify putative cis-regulatory sequences that may be capable of facilitating specific promoter-enhancer interactions at the bithorax complex and propose a model for their molecular function during development. (nih.gov)
  • Molecular Plant-Microbe Interactions. (illinois.edu)
  • In this study, we have investigated basal regulation and myogenic induction of promoter A. Interactions between Ap2 and Sp1 and their cognate DNA motifs are critical for basal transcription from the minimal promoter region. (ox.ac.uk)
  • The team created a set of promoters in Escherichia coli in which promoter strength, transcription factor binding strength, and transcription factor copy numbers were systematically varied, and used mRNA fluorescence in situ hybridization to watch how these differences affected gene expression. (genomeweb.com)
  • These regulatory modules include promoters, insulators, silencers, enhancers, promoter targeting sequences and the recently identified promoter tethering element (PTE). (nih.gov)
  • In transgenic organisms, genetic analysis is used to determine the presence and expression of the foreign DNA sequences that have been introduced into the genome. (tutorialspoint.com)
  • Another method of genetic analysis is Southern blotting, which is used to detect the presence and copy number of specific DNA sequences in an organism's genome. (tutorialspoint.com)
  • Circular dichroism spectroscopy revealed the ability of sequences of this GC-rich region to form guanine-quadruplex structures in vitro. (umn.edu)
  • Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. (lookformedical.com)
  • We demonstrate that one of these cis-acting sequences, HMRE, is able to switch off at least two nonmating-type promoters. (ox.ac.uk)
  • Regulation of gene expression involves post-translational modifications to histones which cause chromatin decondensation and recruit transcription factors at the promoter to initiate transcription. (utexas.edu)
  • Increased acetylation at conserved control elements in the promoter is responsible for the transcriptional up-regulation of the gene following ethanol sedation. (utexas.edu)
  • It is because of the complexity of transcriptional events, which may be a mixture of epigenetic regulation and genetic control on function regions (e.g., enhancers and promoters). (aporc.org)
  • The identified epigenetic regulation and genetic variation will be validated by CRISPR and TALE-TET1 genome editing techniques. (aporc.org)
  • To do so, she is examining in greater detail how experiences can alter the molecular regulation of systems involved in stress and mental health. (asu.edu)
  • The aim of this study was to determine the possible genetic regulation of plasma pro-NT. (lu.se)
  • Transient transfection of promoter-chloramphenicol O-acetyltransferase reporter constructs into primary human connective tissue fibroblasts shows that a 904 bp fragment that hybridizes to a murine TIMP-1 promoter fragment contains a functional promoter. (uea.ac.uk)
  • Pharmacogenetic studies have been also performed in order to evaluate the role of host genetic variants in the prediction of recurrence risk and response to adjuvant treatment with FLs and OXA. (frontiersin.org)
  • However, functional repercussion of such variants at a molecular level has seldom been investigated. (fiocruz.br)
  • Human studies have identified a number of high-risk genetic variants for these diseases. (bvsalud.org)
  • However, due to the paucity of experimental models, molecular alterations induced by these genetic variants and how they correlate to behavioral deficits have not been examined. (bvsalud.org)
  • FINDINGS: We revealed that marmosets bearing different SLC6A4 variants exhibit distinct microRNAs signatures in a region of the prefrontal cortex whose activity has been consistently altered in patients with depression/anxiety. (bvsalud.org)
  • INTERPRETATION: Our findings establish links between genetic variants, molecular modifications in specific cortical regions and complex behavioral responses, providing new insights into gene-behavior relationships underlying human psychopathology. (bvsalud.org)
  • Also in Science , researchers from the University of Toronto detail a new computational model that can predict how strongly difference genetic variants affect RNA splicing, which is mutated in a variety of diseases. (genomeweb.com)
  • Diversity of the association of serum levels and genetic variants of MHC class I polypeptide-related chain A with liver fibrosis in chronic hepatitis C. (cdc.gov)
  • The NT gene (NTS) was sequenced for potential functional variants, evaluating its entire genomic and potentially regulatory regions, in DNA from 28 individuals, stratified by low and high pro-NT levels. (lu.se)
  • UMOD risk variants identified in the above-mentioned GWAS are located in the promoter region of the gene, leading to a theory that they altered UMOD expression. (medscape.com)
  • The insert contains the CoMV promoter region, a codon optimised cp4epsps coding sequence, nptII, aad (that is not expressed in plants) and a portion of ori-V coding regions. (cbd.int)
  • A total of seven sequence variations across the NTS gene, none of them located in coding regions, were identified. (lu.se)
  • Enhancers can usually function in either orientation and at various distances from a promoter. (lookformedical.com)
  • In common with enhancers, it is able to function in either orientation, relatively independently of its position with respect to the regulated promoter, and can act on promoters 2600 bp away. (ox.ac.uk)
  • The ci gene in ciD is controlled by the ubiquitous pan promoter and encodes a hybrid Ci protein that carries the N-terminal region of Pan. (uzh.ch)
  • For example, a promoter region can be fused to a gene encoding a fluorescent protein, and the expression of the protein can be visualized using fluorescence microscopy. (tutorialspoint.com)
  • These processes are responsible for genetic continuity and protein synthesis, enabling life as we know it. (aboutdarwin.com)
  • The +21/+58 region contains a putative binding site for the transcription factor leader-binding protein 1 (LBP-1). (uea.ac.uk)
  • Gel-shift analysis shows that protein binds specifically to this region, but competition studies suggest that it is unlikely to be LBP-1. (uea.ac.uk)
  • To study the cis -regulatory elements present in the promoter region of Hd3a , we generated transgenic plants carrying the 1.75-kb promoter fragment of Hd3a that was fused to the β-glucuronidase ( GUS ) reporter gene. (molcells.org)
  • Expression of a reporter gene, whose transcription was driven by a 1.3 kb promoter A fragment, paralleled expression of the endogenous transcript. (ox.ac.uk)
  • The region hybridizing with a murine TIMP-1 promoter fragment has been subcloned and analysed further. (uea.ac.uk)
  • To date, the central role of specific transcription factors in positively regulating these distinct differentiation processes to acquire a B cell-specific genetic program is well established. (eur.nl)
  • Genetic and molecular data indicate that wild-type Pan and CiD compete for binding to Arm, leading to a compromised transduction of the Wg signal in heterozygous ciD/+ animals and to a dramatic enhancement of the gain-of-function activity of CiD in homozygous mutants. (uzh.ch)
  • In Science this week, researchers from the California Institute of Technology report data showing that variability in gene expression between genetically identical cells arises in predictable ways that are based on promoter architecture. (genomeweb.com)
  • Dr Heidi Mattock and use genetic data to identify the etiology of human canceRs. (who.int)
  • Modern genetic techniques have helped us hugely in characterizing these disorders and providing a molecular diagnosis in the face of nonspecific clinical data. (medscape.com)
  • In an established line of rat fibroblasts (Rat-2), the more distal myc promoter (P2) is the preferred site of initiation. (lu.se)
  • EMSA and dual-luciferase reporter assays further confirmed that STM3 could directly bind the promoter region to activate FUL1 expression. (nature.com)
  • For the hybridization assays, the region of the target gene associated with resistance is PCR amplified, and the labeled PCR products hybridized to oligonucleotide probes immobilized on a nitrocellulose strip or in a microarray. (cdc.gov)
  • Myogenic induction mapped to a conserved upstream muscle-specific E-box, which was shown to bind myogenic regulatory factors, transactivating the promoter up to 18-fold in transient assays. (ox.ac.uk)
  • Molecular Diagnostic and Prognostication Assays for the Subtyping of Urinary Bladder Cancer Are on the Way to Illuminating Our Vision. (who.int)
  • Among them, FRUITFULL1 ( FUL1 ) is downregulated in stm3cr mutants, and its promoter is bound by STM3 by ChIP-qPCR analysis. (nature.com)
  • These results and further mapping with 5' deletion mutants from the -738/+95 region have demonstrated that an AP-1 site at -92/-86 is essential for basal expression of the gene. (uea.ac.uk)
  • Transgenic organisms are living organisms whose genetic material has been artificially modified to introduce specific traits or characteristics. (tutorialspoint.com)
  • This can occur through gene flow, which is the transfer of genetic material from transgenic organisms to wild populations. (tutorialspoint.com)
  • To overcome these drawbacks, rapid genetic DSTs have been developed that shorten the turn-around time from months to several hours.The rapid diagnosis of drug resistance by molecular methods is essential to immediately initiate effective antibiotic therapy, and thus improve treatment outcomes and reduce the transmission of MDR TB. (msdmanuals.com)
  • Mutational analysis indicates these sites are required for AP-1 responsiveness and full promoter activity. (bath.ac.uk)
  • My research interest is in the patient-specific analysis by considering the variations from transcriptional, epigentic, and genetic variations. (aporc.org)
  • Rapid genetic analysis of x-linked chronic granulomatous disease by high-resolution melting. (lu.se)
  • Molecular analysis of X-linked chronic granulomatous disease in five unrelated Korean patients. (lu.se)
  • Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. (lookformedical.com)
  • Deletion analyses of the promoter region indicated that regulatory elements determining vascular cell-specific expression are present in the 200-bp region between -245 bp and -45 bp from the transcription initiation site. (molcells.org)
  • This requires a combination of genetic, molecular, and ecological analyses. (tutorialspoint.com)
  • Our study may be useful for the further meta-analyses related to the genetic predisposition to PCOS. (archive.org)
  • Our aim was to integrate our previously reported clinical-genetic prognostic score with new immunogenetic markers of 5-year disease-free survival (DFS) to evaluate the recurrence risk stratification before fluoropyrimidine (FL)-based adjuvant therapy. (frontiersin.org)
  • Using the binary GAL4/UAS system to express slowpoke in different parts of the Drosophila nervous system I show that slowpoke induction in the mushroom bodies of the adult fly brain, a region critical for olfactory learning and memory, is important for ethanol tolerance. (utexas.edu)
  • A survey of brain structures mediating drug tolerance in the genetically tractable Drosophila could potentially reveal evolutionarily conserved molecular pathways regulating tolerance. (utexas.edu)
  • These findings suggest a mechanism by which a single genetic variant may cause a broad range of phenotypic consequences. (ox.ac.uk)
  • Strikingly, the majority of initiation events occur in regions with enhancer-like chromatin signatures. (nih.gov)
  • It is well known that anesthetics and abused drugs preferentially modulate specific molecular pathways in defined regions of the brain. (utexas.edu)
  • Using CRISPR/Cas9, the scientists edited human embryonic kidney cell lines to mimic Cameron's genetic microdeletion and show how it affects cellular pathways. (scienceboard.net)
  • Large deletions involving entire single exons or multiple exons, large insertions and other complex genetic events have been reported in PTEN and will not be identified using this test methodology. (cincinnatichildrens.org)
  • p30C/EBP alpha binds to the C/EBP sites within and activates reporter gene expression driven by the 422(aP2) and C/EBP alpha gene promoters. (duke.edu)
  • The AHR promoter-luciferase reporter plasmid pGL3-hAhRP was a kind gift from Drs. Sandra Wolff and Josef Abel of the Medical Institute of Environmental Hygiene at the Heinrich-Heine-University, Düsseldorf, Germany. (umn.edu)
  • The TNF gene is encoded in the class III region of the HLA complex adjacent to HLA-B. This genetic proximity to a gene that is already widely implicated in disease susceptibility led us to investigate the association between TNF promoter polymorphisms and susceptibility to BD. (ox.ac.uk)
  • Susceptibility to mycobacterial infections in children with X-linked chronic granulomatous disease: a review of 17 patients living in a region endemic for tuberculosis. (lu.se)
  • Distinct germline genetic susceptibility profiles identified for common non-Hodgkin lymphoma subtypes. (who.int)
  • Two independently regulated promoters control utrophin expression and the upstream promoter (promoter A) is synaptically regulated in muscle. (ox.ac.uk)
  • In this regard, marmosets have emerged as a powerful model in translational neuroscience to investigate molecular underpinnings of complex behaviors. (bvsalud.org)
  • Frontiers in Molecular Neuroscience. (ncku.edu.tw)
  • While a full complement of open chromatin, epigenetic, and TF-binding experiments provide information for likely candidate regulatory regions, using TF-binding approaches such as ChIP-seq or DAP-seq can provide additional valuable insight and targets for reverse genetic approaches such as EMS-induced or natural SNP variant screens or CRISPR editing techniques (e.g. promoter bashing). (usda.gov)
  • Nous avons donc étudié l'association de deux polymorphismes - R353Q au niveau du codon 353 intervenant sur la région catalytique et le polymorphisme d'insertion de 10 paires de bases (pb) impliquant la région du promoteur - avec les taux de FVIIc chez 176 Tunisiens en bonne santé. (who.int)
  • Recent studies have found that they have clinical and molecular features of both the BRAF mutant/MSI and the conventional BRAF wild-type cancers and comprise a distinct colorectal cancer subgroup. (hindawi.com)
  • BRAF mutant/MSS cancers form a distinct colorectal cancer entity that shares clinical and molecular features with both BRAF mutant/MSI serrated pathway cancers and the BRAF wild-type cancers of the conventional pathway [ 9 - 11 ]. (hindawi.com)
  • The plasmid vector PV-GHGT07 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. (cbd.int)
  • The integration of IFNG -rs1861494 in our previous clinical genetic multiparametric score of DFS improved the patients' risk stratification (Log-rank P = 0.0026 in the pooled population). (frontiersin.org)
  • In-house PCR-based tests using molecular beacons have also been used for diagnostic purposes in a few clinical laboratories. (cdc.gov)
  • The relationships between the Arterial and venous thrombosis, which clinical presentation and FVII levels and the clinically manifest as stroke, myocardial in- associated molecular genetic defects lack farction or pulmonary embolism are a ma- apparent consistency [ 7,10 ]. (who.int)
  • Chronic granulomatous disease in Latin American patients: clinical spectrum and molecular genetics. (lu.se)