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Chromatography, GelMolecular WeightChromatography, Ion ExchangeElectrophoresis, Polyacrylamide GelChromatography, AffinityChromatography, High Pressure LiquidChromatographyMolecular Sequence DataKineticsAmino Acid SequenceGelsAmino AcidsSubstrate SpecificityHydrogen-Ion ConcentrationIsoelectric PointIsoelectric FocusingChromatography, DEAE-CelluloseEscherichia coliMacromolecular SubstancesFiltrationChromatography, LiquidCattleSolubilityProtein BindingUltracentrifugationRecombinant ProteinsCarbohydratesChromatography, AgaroseTemperatureDimerizationEnzyme StabilityGlomerular Filtration RateRabbitsHot TemperatureChromatography, GasCloning, MolecularProtein ConformationBacterial ProteinsOligosaccharidesImmunodiffusionBase SequenceCentrifugation, Density GradientSequence Homology, Amino AcidCarbohydrate SequenceGlycoside HydrolasesHydrolysisMass SpectrometryHydroxyapatitesGlycoproteinsUltrafiltrationTrypsinCations, DivalentCircular DichroismChromatography, Thin LayerChemistryChemical PhenomenaEdetic AcidPeptide FragmentsAgaricalesDetergentsPeptide HydrolasesRadioimmunoassayChemical FractionationLiverBinding SitesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationCarrier ProteinsMicroscopy, ElectronDurapatitePeptidesHyperprolactinemiaProtease InhibitorsCytosolEndopeptidasesCross-Linking ReagentsProteinsbeta-GlucosidaseBiological AssayCell LineMagnetic Resonance SpectroscopyProtein DenaturationSwineCell MembraneIsoenzymesStreptomycesSpectrometry, FluorescencePhospholipidsCarbohydrate ConformationRats, Inbred StrainsBlotting, WesternSequence AlignmentImmune SeraPolysaccharidesKidneySpectrophotometryBiopolymersProtein Structure, QuaternaryEnzyme ActivationPrecipitin TestsLectins
AffinityPermeation chromatographyAnion exchangeSubunit molecularFractionationLiquid ChromatographicAgaroseHPLCPurificationProteinsCelluloseMonosaccharideAmmonium SulphateSilica gelResinAverage molecular weightSeparationPolysaccharideBiologyAnalyticalInertDEAEProteinMechanismsStationary phaseEnzymatic hydrolysisEscherichiaFractionAnalyzing the molecular structureWeight markerWeightsBiochemicalOligomersMechanismColumnMethodsMembraneNativeRelevant molecularExchangeElectrophoresisSensitivityMassCompoundsSizeAnalysis revealedMethodPeakFormDiseasesStructureTechniqueColumnsResearchDetection
Affinity7
  • Affinity chromatography (2', 5'-ADP Sepharose 4B) first used by De Flora [20] is a common tech- nique for purification of glucose-6-phosphate dehydrogenase. (scirp.org)
  • The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
  • The elicitor-inducible chitinase was purified by ammonium sulfate fractionation, chitin affinity chromatography and gel filtration. (elsevierpure.com)
  • dc.title: Evaluating Improvements and Challenges in Affinity Chromatography for AAV Purification dc.description.abstract: Purification process development is crucial to the Research and Development (R&D) sector in the biotechnology industry by designing and optimizing downstream unit operations capable of manufacturing safe treatments for clinical trials. (jhu.edu)
  • One of the key polishing steps in the downstream manufacturing process of AAVs is affinity chromatography, where a ligand with specific binding affinity to an AAV serotype is coupled to a base matrix that separates AAVs from host cell contaminants produced in the cell culture process. (jhu.edu)
  • Column resin is the most common affinity chromatography matrix, but it possesses mass transfer limitations. (jhu.edu)
  • Recombinant Mpn HPRT was expressed as an N-terminal fusion protein with a 6 × histidine tag and a tobacco etch virus (TEV) cleavage site at the N-terminus, and was purified to >98% purity by metal affinity chromatography, see more as assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis (data not shown). (bcl-2protein.com)
Permeation chromatography1
Anion exchange2
Subunit molecular2
Fractionation2
  • The enzyme was purified 123.69 fold with a yield of 21.37% by ammonium sulphate fractionation, PEG-4000 precipitation, CM cellulose column chromatography and DEAE cellulose column chromatography. (scirp.org)
  • Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. (sigmaaldrich.com)
Liquid Chromatographic1
  • The Column Chromatography or Liquid Chromatographic systems were a time-consuming method of separation due to the lower solvent flow rate because it was mainly dependent on gravitational force. (tech-publish.com)
Agarose7
  • GoldBio offers a large selection of agarose beads for size exclusion chromatography, which are excellent for your unique research needs. (goldbio.com)
  • Agarose is a very inert polysaccharide that forms hydrophilic and high gel strength gels at low concentrations. (goldbio.com)
  • Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. (goldbio.com)
  • Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. (goldbio.com)
  • Plain agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization. (goldbio.com)
  • Cross-linked agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization. (goldbio.com)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
HPLC4
  • Shim-pack GIS series columns are packed with high purity silica gel and are your ideal generic HPLC columns. (shimadzu.com)
  • The PFY-147-EPS was found to be a levan containing a backbone of 2,6-substituted ß-fructoses by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectroscopy. (bvsalud.org)
  • To understand the history of HPLC, we first needs to understand the history of Liquid chromatography. (tech-publish.com)
  • High Performance Liquid Chromatography (HPLC) is also known as high-performance liquid chromatography or high pressure liquid chromatography. (tech-publish.com)
Purification3
Proteins3
Cellulose2
  • He isolated an inducible extracellular homogeneous enzyme, which shows a 7.5-fold increases in its activity after DEAE cellulose column chromatography. (wikipedia.org)
  • A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (lookformedical.com)
Monosaccharide1
  • The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses. (nih.gov)
Ammonium Sulphate1
Silica gel4
  • Shim-pack MAqC-ODS I reversed-phase columns are packed with a silica gel containing metal and added octadecylsilyl group. (shimadzu.com)
  • Shim-pack WAX/WCX series columns are chemically-bonded hydrophilic silica gel based ion exchange columns. (shimadzu.com)
  • The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID. (lookformedical.com)
  • Shim-pack Diol series is packed with porous spherical silica gel chemically bonded with a hydroxyl group. (shopshimadzu.com)
Resin2
  • It is a gel filtration resin developed for industrial-scale separation of large molecules and virus particles utilizing higher pressure and flow rates rather than low pressure standard beads. (goldbio.com)
  • Desalting and buffer exchange are two of the most common gel filtration chromatography applications, and they can be performed using the same resin. (ichacha.net)
Average molecular weight2
Separation4
  • Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes. (shimadzu.com)
  • The modern definition of chromatography is, it is a physicochemical technique of separation in which the compounds that required to be separated are distributed between two phases, one is called stationary phase (which remains stationary), and the other is a mobile phase (which moves through the stationary phase). (tech-publish.com)
  • The separation happens on the basis of their molecular structure and molecular composition. (tech-publish.com)
  • The technique of GPC does not utilize such chemical reactions as partition, adsorption, and ion exchange, but a physical reaction consisting of a separation based on molecular size of the sample components. (shopshimadzu.com)
Polysaccharide3
  • A low molecular-weight polysaccharide named BIWP2 was purified from the fruit bodies of Bulgaria Inquinans (Fries) via hot-water extraction, followed by freeze-thawing and gel filtration chromatography on Sephadex G-75. (unboundmedicine.com)
  • High performance size exclusion chromatography (HPSEC) showed that it was a homogeneous polysaccharide fraction. (unboundmedicine.com)
  • The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method. (bvsalud.org)
Biology1
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
Analytical1
Inert1
  • Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
DEAE1
Protein4
  • Protein Chromatography. (dcu.ie)
  • The anti-inflammatory activity of CWE was mainly contributed by the protein component of the high molecular weight (HMW) fraction and it exhibited strong inhibitory effect on TNF-alpha production but the possibility of synergistic effect between HMW, MMW and LMW fractions cannot be excluded. (biomedcentral.com)
  • We have partially purified phosphotransferase enzyme I and have purified a heat-stable phosphocarrier protein (HPr) to apparent homogeneity, by gel filtration and ion-exchange chromatography from the soluble fraction. (ox.ac.uk)
  • This format using the molecular mass protein for indirect detection of tetracycline resulting in the highest test sensitivity and was used for further studies. (uni-wuppertal.de)
Mechanisms1
  • Cellular and molecular mechanisms of chemically induced renal carcinogenesis. (cdc.gov)
Stationary phase1
Enzymatic hydrolysis1
Escherichia2
Fraction1
Analyzing the molecular structure1
  • AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes. (molvis.org)
Weight marker1
Weights1
  • The DDA and molecular weights of CTS-OS were determined by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-TOF MS) analysis. (mdpi.com)
Biochemical1
  • Despite decades of clinical, biochemical, and animal model studies, the molecular mechanism of ABCA4 is unknown. (elifesciences.org)
Oligomers2
Mechanism3
  • Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
  • Through the development of m6A RNA sequencing, the relevant molecular mechanism of m6A modification has gradually been revealed. (biomedcentral.com)
  • This review may provide new insights for the molecular mechanism, early diagnosis, histologic grading, targeted therapy and prognostic evaluation of gliomas. (biomedcentral.com)
Column4
Methods1
Membrane2
  • 1990) with the help of membrane ultra filtration and C-75 gel chromatography. (wikipedia.org)
  • A molecular understanding of membrane traffic has broad implications for our understanding of growth control in cancer, receptor trafficking errors in heart disease, regulation of insulin secretion in diabetes and synaptic vesicle biogenesis and transport in neurological disorders. (stanford.edu)
Native2
  • It exhibited homodimeric structure with a molecular weight of native form of 32 kDa and subunits of 16.0 kDa. (springeropen.com)
  • Analysis of gel-filtration chromatography of the RuBPCase synthesized in E. coli revealed that it had a hexadecameric form like the native enzyme of T. ferrooxidans. (elsevierpure.com)
Relevant molecular1
  • We constructed a genetically inactivated or attenuated strain containing relevant molecular assay targets and tested to compare assay performance using this strain to the historical data obtained using irradiation-inactivated virulent spores. (cdc.gov)
Exchange1
Electrophoresis3
  • Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (lookformedical.com)
  • Although migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), under denaturing conditions, was unusually slow, molecular weight determination by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) confirmed that the product was consistent with its theoretical size. (ox.ac.uk)
  • In parallel, all samples were analyzed by denaturing gradient gel electrophoresis (DGGE) of V3-16S rRNA gene amplicons. (who.int)
Sensitivity1
Mass4
  • It has a molecular mass of 66.5 kDa. (sigmaaldrich.com)
  • A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)
  • Analysis of culture media constituents from each cell type by high performance liquid chromatography-mass spectrometry and western blot demonstrated a time-dependent decrease in apelin-55 levels. (phoenixpeptide.com)
  • 1992. Determining volatile organic compounds in human blood from a large sample population by using purge and trap gas chromatography/mass spectrometry. (cdc.gov)
Compounds1
Size1
  • The application of FOS with controlled molecular size (degree of polymerization (DP) of 3 to 6) would result in an increased colonic persistence of the prebiotic effect and reduce the risk of chronic disease of distal intestinal region (van de Wiele et al. (justia.com)
Analysis revealed1
Method1
  • Although providing a pure material, the association of these two steps is time consuming and a single-step method using high performance chromatography media would be useful. (embrapa.br)
Peak1
Form1
  • In the brain of both species, cerebellin-like immunoreactivity consisted of a single molecular form with an elution position on gel filtration and high-performance liquid chromatography identical to that of synthetic rat cerebellin. (ox.ac.uk)
Diseases1
  • The development of molecular imaging at the end of the 20th century has provided a novel visualization for early and non-invasive diagnoses of diseases. (molvis.org)
Structure3
  • The molecular structure of probes is easily visualized under AFM. (molvis.org)
  • Using molecular modelling and the crystal structure of the human cytosolic sialidase NEU2 as a template, the 3D models of the sialidases from zebrafish have been generated. (unibs.it)
  • We investigated the molecular structure of xylooligosaccharides released from corn cob in a continuous flow type hydrothermal reactor designed in our group. (inrae.fr)
Technique1
Columns2
Research1
  • Liquid chromatography was invented in the early 1900s by the Russian botanist, Mikhail S. Tswett, born in 1872 in Italy, during his research on plant pigments. (tech-publish.com)
Detection1
  • The bedrock of successful biodetection platforms and sensors is use of well-characterized molecular assays, immunoassays, or other types of detection assays. (cdc.gov)