Molecular dna. Medical search. Frequent questions

According to a study published today in Nature Structural and Molecular Biology , a protein called UV-DDB -- which stands for ultraviolet-damaged DNA-binding -- is useful beyond safeguarding against the sun. (sciencedaily.com)
It identifies DNA damage so that another crew can come in and patch and seal it," said study senior author Bennett Van Houten, Ph.D., professor of pharmacology and chemical biology at the Pitt School of Medicine and UPMC Hillman Cancer Center. (sciencedaily.com)
Understanding DNA's molecular structure is crucial for grasping genetics and cellular biology. (khanacademy.org)
2019) Nature Structural and Molecular Biology. (cmu.edu)
I would like to receive email from MITx and learn about other offerings related to Molecular Biology - Part 1: DNA Replication and Repair. (edx.org)
Do you feel confident in your ability to design molecular biology experiments and interpret data from them? (edx.org)
7.00x Introduction to Biology and 7.05x Biochemistry or similar (biochemistry, molecular biology, and genetics). (edx.org)
It's wonderful to see Dan Branton recognized for inventing and developing this transformative technology," says Rachelle Gaudet , Professor and Chair of Molecular and Cellular Biology. (harvard.edu)
From a molecular biology point of view this nano-scale self-assembling superstructure could increase the stability of DNA against the nucleases during collagen diseases and the growth of collagen fibrils in the presence of DNA. (scialert.net)
According to molecular biology data, the assembly of the complex between collagen and DNA determines the stability of DNA against the nucleases and indicates a development of autoimmune reaction (Poverenny, 1968). (scialert.net)
The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load and for other molecular biology applications. (atcc.org)
The molecular interactions of genes and gene products underlie fundamental questions of biology. (lu.se)

Molecular models of DNA structures are representations of the molecular geometry and topology of deoxyribonucleic acid (DNA) molecules using one of several means, with the aim of simplifying and presenting the essential, physical and chemical, properties of DNA molecular structures either in vivo or in vitro. (wikipedia.org)
DNA molecular dynamics modeling involves simulating deoxyribonucleic acid (DNA) molecular geometry and topology changes with time as a result of both intra- and inter- molecular interactions of DNA. (wikipedia.org)
DNA, short for deoxyribonucleic acid, consists of nucleotides forming a double helix structure. (khanacademy.org)
A series of model compounds which include methylating and ethylating agents were used to determine the nature and the frequency of different types of deoxyribonucleic acid (DNA) damage caused by the chemicals. (europa.eu)
Deoxyribonucleic acid (DNA) is a complex molecule of many components. (cdc.gov)

High molecular weight genomic DNA is often viscous leading to challenges in handling, transferring, and measuring. (neb.com)
Total genomic DNA was extracted from dried leaf tissue using as DNeasy® 96 Plant Kit (Cat. (uni-goettingen.de)
Quantitative genomic DNA from Pseudomonas aeruginosa strain PAO1-LAC can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. (atcc.org)
To download a certificate of analysis for Quantitative Genomic DNA from Pseudomonas aeruginosa strain PAO1-LAC ( 47085DQ ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
The certificate of analysis for that lot of Quantitative Genomic DNA from Pseudomonas aeruginosa strain PAO1-LAC ( 47085DQ ) is not currently available online. (atcc.org)
EC 2.1.1.6) was determined from rat cDNA and genomic libraries were screened with DNA probes and specific antiserum. (lu.se)

From DNA to Diversity, Molecular Genetics and the Evolution of Animal Design. (bioone.org)
Take a behind-the-scenes look at modern molecular genetics, from the classic experimental events that identified the proteins involved in DNA replication and repair to cutting-edge assays that apply the power of genome sequencing. (edx.org)
Genetic genealogy is the application of genetics to traditional genealogy and is a component of ancestry research experiencing strong growth through personal DNA testing and online ancestry databases. (thegeneticgenealogist.com)

Like other large molecules and biopolymers, DNA often exists in multiple stable geometries (that is, it exhibits conformational isomerism) and configurational, quantum states which are close to each other in energy on the potential energy surface of the DNA molecule. (wikipedia.org)
Using single-molecule imaging, researchers witness how molecules find and fix damaged DNA. (sciencedaily.com)
For the first time, Van Houten's team witnessed this molecular tango along a "tightrope" of DNA slung between two silica beads, using real-time, single-molecule imaging. (sciencedaily.com)
Also, a pyrimidine is always H bonded to a purine so that the diameter/width of the DNA molecule is consistent throughout the molecule. (khanacademy.org)
Finally, the enzyme ligates the broken ends of the DNA strands back together and produces a locally relaxed DNA molecule. (jove.com)
It creates a single-strand breakthrough which the opposite strand can pass, resulting in a locally untangled DNA molecule. (jove.com)
Usually DNA remains as a giant molecule packed in the cells nucleus and is composed of proteins in the chromatin fibers. (scialert.net)
This video will describe how these four groups build upon each other to create the DNA molecule. (cdc.gov)
This pendant is based on the structure of B-DNA, the most common form of this important molecule. (ontogenie.com)

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Although such initial studies of DNA structures with the help of molecular models were essentially static, their consequences for explaining the in vivo functions of DNA were significant in the areas of protein biosynthesis and the quasi-universality of the genetic code. (wikipedia.org)
And, if the DNA in each and every cell is two meters long, how is this genetic material compacted to fit inside the cell nucleus without becoming a tangled mess? (edx.org)
The expected nature of the induced genetic changes can be confirmed by molecular analysis of the DNA sequence changes. (europa.eu)
These results will have future implications in forensic medicine-reconstructing the criminal's face from DNA-and in genetic diagnosis-the photo of the patient's face will already give you clues as to which genome he or she has," Esteller says. (scitechdaily.com)
Today, the Sorenson Molecular Genealogy Foundation (SMGF) reported that they are adopting a standardized Y-STR reporting system proposed by the National Institute of Standards and Technology (NIST) of the U.S. Dept. of Commerce and supported by the International Society of Genetic Genealogy (ISOGG). (thegeneticgenealogist.com)
SALT LAKE CITY (Aug. 17, 2009)-The Sorenson Molecular Genealogy Foundation (SMGF) today announced it has implemented a recently developed industry-standard format for expressing Y-chromosome DNA profiles in genetic genealogy. (thegeneticgenealogist.com)
The new system, or nomenclature, for Y-STR genetic markers will reduce confusion for genetic genealogy consumers, eliminate conversion errors, make personal DNA profiles easily portable and lead to more genetic matches when searching among different ancestry databases once the industry-wide standard is adopted. (thegeneticgenealogist.com)
DNA profiles will become more useful to consumers because they will not have to use a conversion process to search different genetic genealogy databases. (thegeneticgenealogist.com)
Until now, genetic genealogy companies and laboratories reported Y-STR genetic markers in DNA profiles to customers in various formats developed for forensic DNA reporting. (thegeneticgenealogist.com)
As DNA testing for genetic genealogy purposes has become common and more people put their DNA profiles into online ancestry databases, the need for a universal format became apparent," said Katherine Borges, ISOGG director. (thegeneticgenealogist.com)
Borges estimates close to one million DNA tests for genetic genealogy purposes have been purchased to date. (thegeneticgenealogist.com)
The three largest U.S. providers of genetic genealogy DNA testing have committed to adopting the new Y-STR reporting standard and Borges expects the others to follow suit. (thegeneticgenealogist.com)
In genetic research, samples often contain a mixture of target DNA and host DNA, making it difficult to discern and analyze the desired genetic information accurately. (goffinmoleculartechnologies.com)
Molzym Depletion addresses this challenge by offering a solution to selectively remove host DNA, leaving behind the genetic material that researchers aim to study. (goffinmoleculartechnologies.com)
By exploiting the differences between the target DNA and the host DNA, this technology enables researchers to enrich their samples with the genetic material of interest. (goffinmoleculartechnologies.com)
Once the mutation is found, prenatal DNA testing, testing for carriers, and pre-implantation genetic diagnosis are possible. (medlineplus.gov)

Polymerase chain reaction (PCR) analysis for microbial DNA serves as an alternative method for identifying infectious agents. (cdc.gov)
Applied DNA Sciences, a leader in polymerase chain reaction (PCR)-based DNA manufacturing for product authenticity and traceability solutions, has entered into a partnership with Molecular Isotope Technologies (MIT), a pioneer and leader in the application of natural-abundance stable-isotopic analysis provided under the trademarks Nature's Fingerprint and IsoPedigree . (innovationintextiles.com)
DNA replication demands extraordinary specificity and efficiency of catalysis from a DNA polymerase. (utexas.edu)
An important study of T7 DNA polymerase performed by Tsai and Johnson using a conformationally sensitive fluorophore (CSF) characterized a conformational change directly and presented a new paradigm for nucleotide selectivity. (utexas.edu)
Yellow crystals of CSF-labeled T7 DNA polymerase with DNA and correct nucleotide (closed complex), incorrect nucleotide (misaligned complex) or no nucleotide (open complex) were grown to good size and diffracted to 3 Å during X-ray data collection. (utexas.edu)
The present studies confirm that incorporation of ara-C into DNA, and not the competitive inhibition of DNA polymerase, is responsible for inducing lethal cellular events. (aspetjournals.org)
The DnaC helicase loader then interacts with the DnaA bound to the single-stranded DNA to recruit the DnaB helicase, which will continue to unwind the DNA as the DnaG primase lays down an RNA primer and DNA Polymerase III holoenzyme begins elongation. (wikipedia.org)
Once priming is complete, DNA polymerase III holoenzyme is loaded into the DNA and replication begins. (wikipedia.org)
The catalytic mechanism of DNA polymerase III involves the use of two metal ions in the active site, and a region in the active site that can discriminate between deoxyribonucleotides and ribonucleotides. (wikipedia.org)
Furthermore, DNA polymerase III must be able to distinguish between correctly paired bases and incorrectly paired bases. (wikipedia.org)
These interactions result in the DNA polymerase III closing around a correctly paired base. (wikipedia.org)

Whereas molecular models of DNA molecules such as closely packed spheres (CPK models) made of plastic or metal wires for skeletal models are useful representations of static DNA structures, their usefulness is very limited for representing complex DNA dynamics. (wikipedia.org)
Although DNA consists of relatively rigid, very large elongated biopolymer molecules called fibers or chains (that are made of repeating nucleotide units of four basic types, attached to deoxyribose and phosphate groups), its molecular structure in vivo undergoes dynamic configuration changes that involve dynamically attached water molecules and ions. (wikipedia.org)
Such varying molecular geometries can also be computed, at least in principle, by employing ab initio quantum chemistry methods that can attain high accuracy for small molecules, although claims that acceptable accuracy can be also achieved for polynuclelotides, and DNA conformations, were recently made on the basis of vibrational circular dichroism (VCD) spectral data. (wikipedia.org)
It also has the ability to find damage buried in chromosomes and help DNA repair molecules go places they otherwise couldn't, the way a helicopter can navigate really hilly areas. (sciencedaily.com)
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. (jove.com)
Moreover, the drug-DNA complexes were analyzed to investigate specific drug-DNA interactions, as well as the role of the solvent water molecules in the drug-DNA binding. (ub.edu)
Researchers in Germany have developed a DNA-based 'crane' that can pick up molecules from a depot and deposit them precisely at a target site. (chemistryworld.com)
The team designed the geometrical arrangement of the molecules on the chip so that the crane pulled the carrier away from the support strand one base at a time - 'unzipping' the DNA. (chemistryworld.com)
The notion of sequencing nucleic acids by drawing single molecules of DNA or RNA through a nanopore was initially considered quite impossible by most people, including most of the expert scientists who reviewed our grant proposals at NIH. (harvard.edu)
Molecular self-assembly is a process in which molecules (or part of molecules) spontaneously form ordered aggregates and involves no human intervention. (scialert.net)
Over at Nanowerk they have a nice reexamination of molecular electronics and highlights recent work being done in Germany that has "demonstrated that rigidly wired molecules can emit light under voltage bias. (ieee.org)

Spartan Bioscience has unveiled the first assays for its molecular diagnostics device and promised further launches in food and water safety testing. (foodnavigator.com)
ATCC ® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. (atcc.org)

The nanofabrication technique, called 'DNA-brick self-assembly,' uses short, synthetic strands of DNA that work like interlocking Lego bricks. (harvard.edu)
Then, using the energy from a second ATP, Type II topoisomerase reseals the broken ends of the DNA strands and finally detaches from the DNA- leaving behind an untangled DNA helix. (jove.com)
Single strand annealing proteins (SSAPs) like Redβ initiate homologous recombination by annealing complementary DNA strands. (nature.com)
A) Individual nanosized objects are picked up from discrete storage sites with a DNA strand at the tip of an AFM cantilever and transferred to a target site (B). The length and binding geometry of the strands are chosen so that a hierarchy of unbinding forces allows the repetition of this process over and over again. (chemistryworld.com)
Nanopore sequencing involves drawing many single strands of DNA through a two-nanometer diameter pore in an otherwise tightly sealed lipid membrane separating two small liquid filled containers. (harvard.edu)
The decades-long collaboration began in the early 1990s when Deamer and Branton began discussing their similar ideas for expediting the sequencing of DNA by drawing single nucleic acid strands through an interface. (harvard.edu)
In doing so, the research has revealed for the first time how two DNA double strands can be entrapped and encircled by the SMC protein complex. (cam.ac.uk)

I thought, why can't we drive a DNA strand to move in a specific pattern, then we just need to recognize the pattern. (genomeweb.com)
Type I topoisomerases are ATP-independent and act by cutting a bond between nucleotides on a single strand of double stranded DNA. (jove.com)
The unbroken DNA strand is then passed through the gap in the broken strand into the upper cavity of the enzyme. (jove.com)
The enzyme creates a double strand break in one loop of the DNA double helix before helping the unbroken loop to pass through this break via an ATP-dependent reaction. (jove.com)
Type I Topoisomerases act on one strand of double-stranded DNA, and they are further divided into three categories: IA, IB, and IC. (jove.com)
Type IA forms a covalent bond with 5' end of the cleaved DNA strand and removes negative supercoils. (jove.com)
Type IB forms a covalent bond with the 3' end of the broken DNA strand and can relax both positively and negatively supercoiled DNA. (jove.com)
The enzyme rotates the cut single-strand around the opposite strand, untwisting the DNA in the process. (jove.com)
Reaction between molecular beacon and cDNA where a) is the cDNA, b) molecular beacon and c) their hybridization into a double strand sequence. (edinst.com)
By mechanism, DNA annealing proteins are divided into ATPases capable of strand invasion (RecA, RAD51) and single strand annealing proteins (SSAPs) that do not utilize ATP 4 . (nature.com)
As opposed to single strand (ss) DNA binding proteins such as SSB and RPA, which protect and occlude ssDNA from recombination, RAD52/Redβ SSAPs promote recombination and share several biochemical similarities. (nature.com)
They fixed a short DNA 'hook' to the AFM tip, designed to form a double helix when it binds to a complementary 'carrier' strand. (chemistryworld.com)
At the start, the carrier strand is attached to a so-called DNA 'chip' or array and forms a double helix with another 'support' strand attached firmly to the chip. (chemistryworld.com)
When the crane swings around to pick up the cargo, the DNA hook binds firmly to the carrier DNA and pulls it away from the support strand. (chemistryworld.com)
This time, a larger part of the carrier DNA binds to the target strand and when the crane moves away, the carrier and hook separates - leaving the carrier stuck to the target. (chemistryworld.com)
The results also demonstrate that the incorporated ara-C residue is not excised from the DNA strand. (aspetjournals.org)
The relative DNA chain-terminating effect of ara-C provides several mechanisms of action that explain internucleotide and chain terminus positioning of ara-C residues, reinitiation of previously replicated DNA segments, and DNA strand or chromosomal breaks. (aspetjournals.org)
As the DNA strand is drawn through the nanopore from the container on the first side of membrane to the container on the second side, the different nucleotide bases (known as A, C, G, T or U) trigger different electrical responses allowing researchers to identify the order or sequence of the bases. (harvard.edu)
Indeed, it took us and our many bright, hardworking postdocs, students, and laboratory technicians some 25 years before the first crude sequence of a strand of DNA was obtained by nanopore sequencing. (harvard.edu)
It is hypothesized that DNA stretching by DnaA bound to the origin promotes strand separation which allows more DnaA to bind to the unwound region. (wikipedia.org)

DNA from 968 blood samples from children in the Study to Explore Early Development (SEED 1) was used to generate epigenome-wide array DNA methylation (DNAm) data at 485,512 CpG sites for 453 cases and 515 controls, using the Illumina 450K Beadchip. (springer.com)
In the present investigation, we studied the modulating effects of several tea catechins and bioflavonoids on DNA methylation catalyzed by prokaryotic SssI DNA methyltransferase (DNMT) and human DNMT1. (aspetjournals.org)
We found that each of the tea polyphenols [catechin, epicatechin, and (-)-epigallocatechin-3- O -gallate (EGCG)] and bioflavonoids (quercetin, fisetin, and myricetin) inhibited SssI DNMT- and DNMT1-mediated DNA methylation in a concentration-dependent manner. (aspetjournals.org)
When epicatechin was used as a model inhibitor, kinetic analyses showed that this catechol-containing dietary polyphenol inhibited enzymatic DNA methylation in vitro largely by increasing the formation of S -adenosyl- l -homocysteine (a potent noncompetitive inhibitor of DNMTs) during the catechol- O -methyltransferase-mediated O -methylation of this dietary catechol. (aspetjournals.org)
In comparison, the strong inhibitory effect of EGCG on DNMT-mediated DNA methylation was independent of its own methylation and was largely due to its direct inhibition of the DNMTs. (aspetjournals.org)
Overall, the results revealed that these individuals share similar genotypes, but differ in their DNA methylation and microbiome landscapes. (scitechdaily.com)
We aimed to investigate whether the molecular subtypes also display distinct methylation profiles. (lu.se)
Conclusions: We have found that breast cancers of the basal-like, luminal A and luminal B molecular subtypes harbour specific methylation profiles. (lu.se)

From the very early stages of structural studies of DNA by X-ray diffraction and biochemical means, molecular models such as the Watson-Crick nucleic acid double helix model were successfully employed to solve the 'puzzle' of DNA structure, and also find how the latter relates to its key functions in living cells. (wikipedia.org)
Structural information is generated from X-ray diffraction studies of oriented DNA fibers with the help of molecular models of DNA that are combined with crystallographic and mathematical analysis of the X-ray patterns. (wikipedia.org)
Brewood, G. P., Aliwarga, T. & Schurr, J. M. A structural transition in duplex DNA induced by ethylene glycol. (nature.com)

The results to date include that measurements of DNA adduct frequencies can give information concerning the mutagenic potency of a genotoxic agent, but these determinations should be accompanied by measurements of the effects of DNA repair mechanisms on the persistence of the DNA lesions. (europa.eu)
Moreover most organisms are equipped with DNA repair mechanisms which remove specific DNA adducts from their genome with varying efficiency. (europa.eu)

An old standing dynamic problem is how DNA "self-replication" takes place in living cells that should involve transient uncoiling of supercoiled DNA fibers. (wikipedia.org)
An in-depth adventure through DNA replication and repair to strengthen your scientific thinking and experimental design skills. (edx.org)
Let's explore the limits of our current knowledge about the replication machinery and pathways that protect the fidelity of DNA synthesis. (edx.org)
If you are up for the challenge, join us in 7.28x Part 1: DNA Replication and Repair. (edx.org)
The unwinding of the DNA double helix during replication results in overwinding in regions ahead of the replication fork. (jove.com)
This twisting inhibits further unwinding of the DNA, stalling vital cell processes such as DNA replication. (jove.com)
MMC induces DNA damage to quiescent corneal keratocytes, which remains unrepaired, resulting in abnormal cell replication and gene transcription that leads to long-term effects on corneal repair. (molvis.org)
These data indicate that the effect of MMC on corneal scarring and haze is related to the generation of DNA ICLs leading to defective cell replication and gene expression. (molvis.org)
Prokaryotic DNA Replication is the process by which a prokaryote duplicates its DNA into another copy that is passed on to daughter cells. (wikipedia.org)
All cells must finish DNA replication before they can proceed for cell division. (wikipedia.org)
For the same reason, the initiation of DNA replication is highly regulated. (wikipedia.org)

The first DNA sequence of a cephalopod was published in 1983 and the first molecular paper focusing on cephalopods was published in 1994. (tonmo.com)
These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. (jove.com)
Molecular beacon probes are a sequence of nucleotides (the building blocks of DNA and RNA) that can be used to fluorescently detect the presence of a specific sequence of DNA or RNA. (edinst.com)
The molecular beacon (Figure 1) is designed so that a small number of nucleotide bases (between 5 and 7) at the sequence terminals are complementary to each other and get paired forming what is called the stem. (edinst.com)
A nucleotide sequence with complementary bases to the molecular beacon loop is called complementary DNA (cDNA). (edinst.com)
In the presence of its cDNA, the molecular beacon hairpin will open and hybridize with the cDNA to form a double stranded sequence (Figure 2). (edinst.com)
On the basis of the results derived from these simulations, several issues related to the sequence-selective binding of elsamicin A to DNA are discussed. (ub.edu)
Molecular phylogeny of tribe Dipterocarpeae (family Dipterocarpaceae) based on sequence data of chloroplast and nuclear DNA. (uni-goettingen.de)
The technology provides real-time information on DNA sequence and is currently being used around the world in both large research laboratories and in field work situations. (harvard.edu)

Comparison of MD simulations of free and DNA-bound elsamicin A provided insight into the mechanism of DNA cleavage. (ub.edu)
This scenario constitutes a new mechanism which explains how one-electron oxidation of the DNA double helix can lead to mutations. (wjgnet.com)
Previous studies on several DNA polymerases suggested that a rate-limiting conformational change preceding chemistry accounts for the high specificity following the induced fit mechanism. (utexas.edu)

The first reports of a double helix molecular model of B-DNA structure were made by James Watson and Francis Crick in 1953. (wikipedia.org)
After the crane arrives in the target area, the 'free' end of the carrier DNA binds to the target DNA - again forming a double helix. (chemistryworld.com)
Interaction between the collagen and DNA leads to destruction of the hydration shell of the triple helix and stabilization of the double helix structure. (scialert.net)
Such extraordinary complex induces distraction hydration shell of collagen triple helix, stabilization hydration shell of ds-DNA, stability of DNA against the nucleases and this complex between collagen and DNA determines unusual properties of collagen fibers. (scialert.net)
High-resolution structure of a DNA helix containing mismatched base pairs. (wikidata.org)
MatP-bound DNA threads through the MukBEF ring, whilst the second helix of DNA is clamped by the proteins MukF, MukE and the MukB ATPase heads. (cam.ac.uk)

The results showed that those 2 isolates were multi-drug resistant and the DNA sequencing analysis showed that the alignment of nucleic acid of DNA in isolates of mycobacteria other than M. tuberculosis was different from that of M. tuberculosis complex. (who.int)

Molecular beacon probes can be custom designed to target specific DNA or RNA sequences, which allows molecular beacons to be used for real-time detection and quantification of DNA and RNA. (edinst.com)
Development of methodologies for the detection of DNA adducts in specific DNA sequences. (europa.eu)
There is evidence that the distribution of DNA adducts over the genome immediately after exposure is not random, but that certain DNA sequences are damaged to a higher extent. (europa.eu)

iii) Isolation and characterization of monoclonal antibodies against DNA containing specific DNA adducts or against a purified DNA adduct itself. (europa.eu)

Supercoiling, packing with histones in chromosome structures, and other such supramolecular aspects also involve in vivo DNA topology which is even more complex than DNA molecular geometry, thus turning molecular modeling of DNA into an especially challenging problem for both molecular biologists and biotechnologists. (wikipedia.org)
In a study published earlier this year in PNAS , Ping and his colleagues found that spacing miniaturized pyramidal structures on the graphene sheet was an optimal approach, as the tethered DNA pieces would not bump each other when waggling in the A/C field. (genomeweb.com)
The strength of the new technique lies in the fact that it is capable of directly "writing" molecular structures on surfaces in a modular and programmable way. (chemistryworld.com)
Last week I highlighted some recent research in the UK that successfully built 3D molecular structures on a surface using buckyballs and the process of self assembly. (ieee.org)

Computer molecular models also allow animations and molecular dynamics simulations that are very important for understanding how DNA functions in vivo. (wikipedia.org)
The more advanced, computer-based molecular models of DNA involve molecular dynamics simulations and quantum mechanics computations of vibro-rotations, delocalized molecular orbitals (MOs), electric dipole moments, hydrogen-bonding, and so on. (wikipedia.org)
Computer molecular modeling allows both animations and molecular dynamics simulations that are very important to understand how DNA functions in vivo. (wikipedia.org)
Such chemical dynamics and biochemical reactions of DNA are much more complex than the molecular dynamics of DNA physical interactions with water, ions and proteins/enzymes in living cells. (wikipedia.org)
Molecular mechanics (MM) and dynamics (MD) were used to examine the conformational flexibility of elsamicin A, an antitumour antibiotic, in aqueous solution. (ub.edu)

Without vigilant repair, cancer would run rampant, and now scientists at the University of Pittsburgh have gotten a glimpse of how one protein in particular keeps DNA damage in check. (sciencedaily.com)
Eliasson, R., Hammarsten, E. & Lindahl, T. Use of ethylene glycol in counter flow electrophoresis and in cold concentration of DNA and protein solutions. (nature.com)
Atomic model for the MukBEF-DNA complex (left) and in vivo loop binding as determined by chemical circularisation of the protein (right). (cam.ac.uk)
Tocilizumab is a protein with a molecular mass of approximately 145 kDa and the Chemical Abstracts Services (CAS) number 375823-41-9. (janusinfo.se)

This paper reveals how the SMC complex organises DNA loops to individualise chromosomes and avoid harmful tangling of DNA. (cam.ac.uk)

Further development of methodologies for the detection and quantification of DNA adducts in the genome overall, which will involve: (i) The use of radioactively labelled model compounds which allow the quantification of labelled DNA adducts at low frequency. (europa.eu)
Soon molecular testing will involve whole genome sequencing on patients and their relatives to better establish the diagnosis. (medlineplus.gov)
Employing the DNA blot analysis only one COMT-encoding gene was found in the rat genome. (lu.se)

Direct link to gayathri vinjamuri's post "single stranded dna is fo. (khanacademy.org)
Because single-stranded and double-stranded DNA have different flexibilities, they move differently, allowing for detection of hybridization. (genomeweb.com)

This thesis describes work to further characterize the underlying molecular basis regulating the conformational change by a combination of site-directed mutagenesis, transient kinetics and crystallography. (utexas.edu)

Hello everyone Does anyone know why in some cases we see (in an 0,8% agarose gel) plasmid DNA higher than chromosomal DNA? (bio.net)
Chromosomal DNA was indeed trapped inside the ring in these modified E. coli cells, suggesting that MukBEF does enclose DNA loops in cells - as observed in the cryo-EM structure. (cam.ac.uk)

1-beta-D-Arabinofuranosylcytosine (ara-C) incorporates into DNA, and the extent of this incorporation correlates significantly with inhibition of DNA synthesis. (aspetjournals.org)
Included topics are (i) absolute and relative quantification of DNA/RNA, (ii) limit of detection, (iii) optimization of PCR, (iv) primer/probe design, (v) contamination and quality control, (vi) validation of PCR methods (vii) sample preparation (DNA/RNA), (viii) understanding and relieving PCR inhibition (pre-PCR processing), and (ix) forensic DNA analysis. (lu.se)

This hydrolysis drives DNA synthesis to completion. (wikipedia.org)

In the new study, Esteller and his research team set out to characterize, on a molecular level, random human beings that objectively share facial features. (scitechdaily.com)

The model suggests that DNA, containing well-arranged phosphate groups, helps the collagen to make ordered aggregates-extraordinary twisted fiber complex. (scialert.net)
According to the molecular model (Mrevlishvili and Svintradze, 2005b) DNA containing well-arranged phosphate groups helps to make this complex and these groups and water layers play key roles during collagen-DNA ordered aggregation in aqueous solution. (scialert.net)

In this application note, nanomolar concentrations of cDNA were quantified using a molecular beacon while controlling the temperature of incubation and measuring the sample emission with an Edinburgh Instruments FS5 Spectrofluorometer. (edinst.com)
The molecular beacon and its cDNA were purchased from Merck. (edinst.com)
The SC-27 was used to heat, while individually stirring, four solutions with different concentrations of cDNA and the molecular beacon. (edinst.com)
Four solutions were analysed with a molecular beacon concentration of 100 nM and varying concentrations of cDNA: 0 nM, 20 nM, 40 nM, and 60 nM. (edinst.com)
Hybridization between the cDNA and molecular beacon can occur while the beacon is in the closed hairpin form but will proceed slowly. (edinst.com)

At the time, the only method available for sequencing DNA involved time consuming expensive biochemical maneuvers and electrophoresis in gels that required a controlled lab environment and heavy, costly equipment. (harvard.edu)
To take the best advantage of long-read sequencing, DNA extraction methods must be able to isolate pure high molecular weight (HMW) DNA from complex metagenomics samples, without introducing any bias. (altmetric.com)
In addition to the comparison of the quality, quantity and purity of the extracted DNA, the performance obtained when doing Nanopore sequencing on a MinION flow cell was also tested. (altmetric.com)
Amongst the 6 tested methods, the Quick-DNA HMW MagBead Kit (Zymo Research) was considered as the most suitable for Nanopore sequencing and would be recommended for bacterial metagenomics studies using this technology. (altmetric.com)
Pacific Biosciences and Advanced Analytical Technologies have signed an agreement to jointly promote technology for long-read DNA sequencing. (foodnavigator.com)

Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
250 kb is easy to handle and can be accurately measured using standard spectrophotometric methods, UHMW DNA measurement can be made more accurate by reducing the overall DNA fragment size of a small aliquot of the sample. (neb.com)
In " A simple approach for effective shearing and reliable concentration measurement of ultra-high-molecular-weight DNA ", a methods paper published in Biotechniques, we outline a simple and effective approach for shearing small aliquots of UHMW DNA samples to a size conducive to accurate spectrophotometric measurement. (neb.com)
v) Further development of immuno histochemical methods for the detection of DNA adducts in various organs and cell types of mammals. (europa.eu)
vii) Further development of post-labelling methods for other than bulky DNA adducts. (europa.eu)
In this study, with different bacterial cocktail mixes, analyzed as pure or spiked in a synthetic fecal matrix, we evaluated the performances of 6 DNA extraction methods using various cells lysis and purification techniques, from quick and easy, to more time-consuming and gentle protocols, including a portable method for on-site application. (altmetric.com)

Back at the lab, Ping and his team found success by tethering DNA probes to nanostructures on graphene sheets and making them vibrate in electric fields. (genomeweb.com)

The properties of high-molecular-weight DNA are usually investigated in neutral aqueous solutions. (nature.com)
In this paper is demonstrated that one example of the biologically important and molecular self-assembling complex system is a collagen-DNA ordered aggregates spontaneously formed in aqueous solutions. (scialert.net)

They are weak ssDNA binding proteins with no affinity for double-stranded (ds) DNA. (nature.com)
In general, DNA-binding proteins associate with the major groove. (ontogenie.com)

Through the combined power of our cutting-edge technologies, Applied DNA offers brand owners one-stop-shop access to unique analytical tools to verify product and process authenticity for brand assurance and provenance with forensic certainty," said Judy Murrah, Chief Information Officer of Applied DNA. (innovationintextiles.com)
Our partnership with Applied DNA offers great specificity in forensic authentication to our joint customers, amplifying product and process claims throughout highly complex global supply chains. (innovationintextiles.com)
The course leader performs research in the areas of diagnostic PCR, forensic DNA analysis, and pre-PCR processing. (lu.se)

Prior to measurement, samples should be properly homogenized following the guidelines in Homogenization of High Molecular Weight DNA (HMW DNA) Samples . (neb.com)

With his team at NIST, Balijepalli has also been pursuing biosensors for DNA detection. (genomeweb.com)
60°C was found to be an adequate temperature for the hairpins to be completely open, with the emission at 60°C being 50 times higher than at 20°C. Based on these results 60°C was chosen as the incubation temperature for DNA detection. (edinst.com)

An X-ray diffraction study of DNA fibers immersed in various media. (nature.com)

The independent controls segment accounted for the biggest share of the global molecular quality controls market in 2022. (bharatbook.com)
Single-analyte controls accounted for the biggest share of the molecular quality controls market in 2022. (bharatbook.com)
The infectious disease diagnostics segment accounted for the biggest share of the global molecular quality controls market in 2022. (bharatbook.com)
North America dominated the molecular quality controls market in 2022. (bharatbook.com)

Tocilizumab, the pharmaceutical active ingredient in RoActemra, is a recombinant humanised immunoglobulin IgG1 monoclonal antibody produced by recombinant DNA technology. (janusinfo.se)

Molecular typing of Mycobacterium spp. (who.int)

They use their built-in motor activity to then extrude large DNA loops and change the three-dimensional path of DNA during important cellular processes. (cam.ac.uk)

The cell recognizes the shape of DNA-collagen complex, and effectively takes up complexes of certain shapes. (scialert.net)
SMC complexes have a ring-like structure, which allows them to entrap DNA. (cam.ac.uk)

Goffin Molecular Technologies is a leading Dutch company with a rich history, offering a diverse range of high-quality molecular diagnostics products from various manufacturers. (goffinmoleculartechnologies.com)

Introduction: Five different molecular subtypes of breast cancer have been identified through gene expression profiling. (lu.se)
In what follows we limit ourselves to the analysis of gene expression measurements obtained using microarrays with small spots of DNA fixed to glass slides. (lu.se)
Levels of gene expression are measured using a preparation of fluorescently labeled tissue RNA (copies of DNA), together with reference RNA labeled with a different fluorochrome, hybridized onto the slides. (lu.se)

While the use of all-electronic transduction of DNA binding using graphene devices is well established, Johnson said he was not aware of other efforts to detect molecular binding by an electromechanical measurement of the biomolecule itself. (genomeweb.com)
Molecular tests were used to detect pathogens. (who.int)

Patients were included for further study if they met the pathologic and clinical features described and if the specimens, after processing and DNA extraction, were positive for human β-actin with PCR analysis. (cdc.gov)
Additionally, the participants completed a comprehensive biometric and lifestyle questionnaire and provided saliva DNA for multiomics analysis. (scitechdaily.com)
The course covers PCR-based DNA/RNA analysis from sampling to evaluation of results, with a specific focus on real-time quantitative PCR (qPCR). (lu.se)

Rosalind Franklin made the critical observation that DNA exists in two distinct forms, A and B, and produced the sharpest pictures of both through X-ray diffraction technique. (wikipedia.org)
The purified MukBEF-MatP complex was shown to bind two distinct DNA helices, corresponding to the two arms of a DNA loop. (cam.ac.uk)

That same year, Maurice F. Wilkins, A. Stokes and H.R. Wilson, reported the first X-ray patterns of in vivo B-DNA in partially oriented salmon sperm heads. (wikipedia.org)
Epigenetic transformation studies of DNA in vivo were however much slower to develop despite their importance for embryology, morphogenesis and cancer research. (wikipedia.org)

DNA binds to collagen directly to form DNA-collagen complex. (scialert.net)

2 purines would make the DNA bulky in parts and 2 pyrimidines would make it very narrow. (khanacademy.org)

Where did the oxygen from 1st carbon in deoxyribose go when in DNA? (khanacademy.org)

This produces a signature waveform, or nanomechanoelectrical fingerprint, of DNA, and hybridization of target DNA oligomers with probe DNA oligonucleotides modulates the current. (genomeweb.com)

As in the DNA tightrope experiment, UV-DDB rushed to the scene, and when it wasn't available, cells were more sensitive to oxidative stress. (sciencedaily.com)
To show that UV-DDB performs the same functions in living cells, Van Houten recruited the help of Marcel Bruchez, Ph.D. , of Carnegie Mellon University's Molecular Biosensor and Imaging Center , and Patricia Opresko, Ph.D. , of Pitt. (cmu.edu)
You're acquainted with your DNA, but did you know that your cells synthesize enough DNA during your lifetime to stretch a lightyear in length? (edx.org)
Parallel to the measurements of DNA damage the frequency of mutations was determined in different organisms which include cultured mammalian cells, mouse germ cells, Drosophila, yeast, Escherichia coli and Salmonella typhimurium. (europa.eu)
a.Determination of DNA adduct frequencies as a function of time in: Different organs of mice, hamsterand rat, including mouse and rat germ cells. (europa.eu)
Therefore, it is expected that when the correlation between the shape of complex and uptake of the complex into the cell is clarified, the efficiency of introduction of DNA into cells with the use of collagen will increase even more. (scialert.net)
However, during some pathological processes, in particular, during collagen diseases, DNA is released from the nucleus and is placed in the matrix between the cells-in the connective tissue (Poverenny, 1968). (scialert.net)
In order to test whether the mode of DNA binding revealed by cryo-EM also occurs in cells, Frank genetically engineered E. coli strains in which the SMC ring could be chemically locked. (cam.ac.uk)
In other words, it is possible that in fast growth conditions the grandmother cells starts replicating its DNA for grand daughter cell. (wikipedia.org)

Double-stranded DNA remains soluble in salt-containing glycol, although it precipitates in polyethylene glycol. (nature.com)
DNA also remains soluble in formamide, but the double-helical structure of DNA is much less stable in this solvent than in glycol. (nature.com)
Can DNA be either single or double stranded? (khanacademy.org)
The arms of the DNA loop are thread through separate compartments of the MukBEF complex, keeping the DNA loop 'double locked' to promote length-wise compaction of the chromosome. (cam.ac.uk)
In addition, dsDNA (double stranded DNA) in the active site has a wider major groove and shallower minor groove that permits the formation of hydrogen bonds with the third nitrogen of purine bases and the second oxygen of pyrimidine bases. (wikipedia.org)

Van Houten]'s team has developed an assay that allows them to track the repair enzymes in 3D on the DNA ropes as they repair damage. (sciencedaily.com)
that is, these enzymes reversibly break then reconnect phosphodiester bonds in DNA to remove torsional strain in overwound DNA. (jove.com)
Molzym Depletion is a sophisticated methodology that utilizes enzymes to digest and degrade non-target host DNA while preserving the integrity of the desired DNA. (goffinmoleculartechnologies.com)

Biology12

Deoxyribonucleic acid5

Genomic6

Genetics3

Molecule9

Pharmacology20

Genetic16

Polymerase11

Molecules11

Assays2

Strands7

Strand20

Methylation8

Structural3

Mechanisms2

Replication11

Sequence9

Mechanism3

Helix6

Nucleic acid1

Sequences3

Characterization1

Structures4

Dynamics5

Protein4

Chromosomes1

Genome3

Single stranded2

Conformational change1

Chromosomal2

Inhibition2

Synthesis1

Characterize1

Phosphate groups2

CDNA5

Sequencing5

Methods6

Probes1

Aqueous solutions2

Proteins2

Forensic3

High molecular1

Detection2

Fibers1

20224

Recombinant1

Mycobacterium1

Cellular1

Complexes2

Diagnostics1

Gene Expression3

Detect2

Analysis3

Distinct2

Vivo2

Binds1

Bulky1

Deoxyribose1

Oligonucleotides1

Cells9

Double5

Enzymes3