Parallel studies of a new human SOX17-eGFP ES cell line allowed analysis of endoderm differentiation in vitro, leading to culture modifications that enhanced expression of an endoderm-like signature. (uthscsa.edu)
Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
We present a predictive bioprocess design strategy employing cell- and molecular-level analysis of rate-limiting steps in human pluripotent stem cell (hPSC) expansion and differentiation, and apply it to produce definitive endoderm (DE) progenitors using a scalable directed-differentiation technology. (nextbigfuture.com)
See More Use our definitive endoderm (DE) differentiation kit to obtain functional DE cells that are capable of downstream differentiation to hepatic, intestinal, pancreatic and pulmonary cells. (stemcell.com)
We determine dose and temporal requirements of signaling pathway engagement for optimized differentiation and characterize the differentiation products on cellular and integrated molecular level. (bvsalud.org)
Differentiation of human embryonic stem cells (ESCs) to END offers a tractable system to study the molecular basis of normal and defective human-specific END development. (bvsalud.org)
The molecular events leading to human embryonic stem cell (hESC) differentiation are the subject of considerable scrutiny. (northwestern.edu)
Reprogramming techniques continue to evolve as we learn more about the developmental and molecular cues which govern cellular differentiation and identity. (silverchair.com)
Embryonic12
Embryonic definitive endoderm (DE) generates the epithelial compartment of vital organs such as liver, pancreas, and intestine. (uthscsa.edu)
However, purification of DE in mammals has not been achieved, limiting the molecular "definition" of endoderm, and hindering our understanding of DE development and attempts to produce endoderm from sources such as embryonic stem (ES) cells. (uthscsa.edu)
Here, we describe purification of mouse DE using fluorescence-activated cell sorting (FACS) and mice harboring a transgene encoding enhanced green fluorescent protein (eGFP) inserted into the Sox17 locus, which is expressed in the embryonic endoderm. (uthscsa.edu)
Maldevelopment of the pharyngeal endoderm, an embryonic tissue critical for patterning of the pharyngeal region and ensuing organogenesis, ultimately contributes to several classes of human developmental syndromes and disorders. (bvsalud.org)
We have used conditional gene deletion of mouse β-catenin in Sox17-positive embryonic and extra-embryonic endoderm as well as vascular endothelial progenitors to address the function of canonical Wnt signalling in cell lineage formation and patterning. (biologists.com)
Understanding these changing states of commitment is a prerequisite for deciphering molecular mechanisms that regulate fate segregation of the embryonic NC. (ehu.eus)
SOX17 plays an essential role in the regulation of embryonic development and is required for normal looping of the embryonic hear tube and for normal development of the definitive gut endoderm. (thermofisher.com)
The researchers used chemicals to prompt the cells to create the definitive endoderm, which is a flat layer of cells that forms early during embryonic development. (livescience.com)
These include embryonic disc and bilaminar disc formation, epiblast lumenogenesis, polarized amniogenesis, anterior-posterior symmetry breaking, primordial germ-cell specification, polarized yolk sac with visceral and parietal endoderm formation, extra-embryonic mesoderm expansion that defines a chorionic cavity and a connecting stalk, and a trophoblast-surrounding compartment demonstrating syncytium and lacunae formation. (nature.com)
This work will provide a definitive laboratory reference for future studies of early embryo development, and the embryonic origins of disease," said Thorsten Boroviak, PhD, principal investigator in the laboratory for primate embryogenesis in the Centre for Trophoblast Research at the University of Cambridge. (genengnews.com)
The fertilized oocyte is totipotent, with resultant blastomeres capable of generating all three primary germ layers, ectoderm, mesoderm, and endoderm, in addition to the extra embryonic tissues. (conditionmed.org)
Scientists in Canada have overcome a key research hurdle to developing regenerative treatments for diabetes and liver disease with a technique to produce medically useful amounts of endoderm cells from human pluripotent stem cells. (nextbigfuture.com)
The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
Foregut endoderm3
Approaches to study human pharyngeal foregut endoderm-a developmental intermediate that is linked to various human syndromes involving pharynx development and organogenesis of tissues such as thymus, parathyroid, and thyroid-have been hampered by scarcity of tissue access and cellular models. (bvsalud.org)
The overall goal of the project is to generate SC-ILCs with superior functionality by modulating the patterning of the endoderm/foregut endoderm. (helmholtzresearchschool-diabetes.org)
To screen small molecules that induce an anterior definitive endoderm/ pancreatic foregut endoderm fate that increases the yield of generated pancreatic progenitors and endocrine cells. (helmholtzresearchschool-diabetes.org)
Sox1710
Comparison of patterns of signaling pathway activation in native mouse DE and endoderm-like cells generated from ES cells produced novel culture modifications that generated Sox17-eGFP + progeny whose gene expression resembled DE more closely than achieved with standard methods. (uthscsa.edu)
A set of markers (GSC, CK18, Gata4, Eomes, and Sox17) point to a mesendodermal nature of the epithelial cells with subsequent commitment to definitive endoderm (Sox17, Cdx2, nestin, and Islet1). (northwestern.edu)
Sox17 lineage tracing in the definitive endoderm (DE) shows a cell-autonomous requirement for β-catenin in midgut and hindgut formation. (biologists.com)
2020), Sox17 and β-catenin co-occupy Wnt-responsive en. (xenbase.org)
Sox17 and β-catenin co-occupy Wnt-responsive enhancers to govern the endoderm gene regulatory network. (xenbase.org)
Sox17 is a key transcriptional regulator of definitive endoderm development, and yet, its genomic targets remain largely uncharacterized. (xenbase.org)
Here, using genomic approaches and epistasis experiments, we define the Sox17 -governed endoderm GRN in Xenopus gastrulae. (xenbase.org)
We show that Sox17 functionally interacts with the canonical Wnt pathway to specify and pattern the endoderm while repressing alternative mesectoderm fates. (xenbase.org)
This solution also worked in vitro differentiated definitive endoderm cells (data not shown). (diagenode.com)
Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
Visceral3
Tetraploid rescue experiments reveal that lack of β-catenin in the anterior visceral endoderm results in defects in head organizer formation. (biologists.com)
Surprisingly, wild-type posterior visceral endoderm (PVE) in midgut- and hindgut-deficient tetraploid chimera rescues the posterior body axis truncation, indicating that the PVE is important for tail organizer formation. (biologists.com)
More specifically, the 3D-transcriptomes showed "the emergence of the anterior visceral endoderm, which is hallmarked by conserved (HHEX, LEFTY2, LHX1) and primate-specific (POSTN, SDC4, FZD5) factors. (genengnews.com)
Cells7
One critical aspect of optical imaging is the 3D localization of molecular composites in tissues or cells, typically by using specific antibodies with fluorescent reporters. (nature.com)
For some cell types, such as Th17 and regulatory T cells (Tregs), definitive identification depends on the combined use of surface and intracellular markers such as cytokines or transcription factors. (bdbiosciences.com)
Therefore, after treatment, cells must be quickly fixed and subjected to stronger permeabilization conditions to allow the antibody to enter the nucleus and access the epitope within disrupted molecular complexes. (bdbiosciences.com)
Specifically, the objectives of this CIRM grant are to elucidate the role miRNAs play in the development of hESC into cells of endocrine lineage and to provide crucial details on the molecular architecture of endocrine precursor populations, lineage specification, and β-cell maturation. (ca.gov)
In the past, biomarkers were defined as 'cellular, biochemical or molecular alterations that are measurable in biological media such as human tissues, cells, or body fluids' [1]. (gene-quantification.org)
L) Statistic analysis of phospho- histone H3-positive cells on the whole surface of bisected vegetal endoderm , as demarcated by the red dashed lines in J and K. As the distribution of phospho-histone H3-positive cells across the whole vegetal endoderm sur - face did not show obvious bias, for simplicity, we counted the whole area rather than only putative vpp1 -positive area. (xenbase.org)
ventral pancreatic precursor 1 ( vpp1 ) is a marker gene that is differentially expressed in a cell population within the dorsal endoderm in a pattern partially overlapping with that of hematopoietically expressed homeobox ( hhex ) during gastrulation. (xenbase.org)
Biology3
Currently, biomarkers span a broad diagnostic sector and have been used since the earliest days of the application of molecular biology to increase our understanding of disease mechanisms. (gene-quantification.org)
Main applications are molecular biology, NGS, CRISPR/Cas9 gene editing, mammalian gene expression, fluorescent proteins, stem cell research and viral transduction tools. (scientifix.com.au)
Reagents and instruments for immunology, cell biology and molecular biology. (biotrend.com)
Lineage1
In addition to their histologic characteristics, germ cell tumors retain molecular characteristics of their primordial lineage. (medscape.com)
Emergence2
Emergence and patterning dynamics of mouse-definitive endoderm. (mpg.de)
To close this gap, we apply transcriptomic and chromatin accessibility single-cell sequencing technologies to generate a multi-omic developmental resource spanning pharyngeal endoderm patterning to the emergence of organ-specific epithelia in the developing mouse embryo. (bvsalud.org)
Pancreatic buds2
Conversely, miR-122 is the most highly expressed miRNA in liver, which arises in the region of endoderm that is closest to the pancreatic buds. (ca.gov)
vpp1 and hhex display partially overlapping expression in gas - trulating dorsal endoderm and later demarcate ventral pancreatic buds and liver anlagen, respectively. (xenbase.org)
Gastrulae1
Liver and ventral pancreas develop from neighboring territories within the endoderm of gastrulae. (xenbase.org)
Formation1
Elucidating the molecular framework of axis formation in vivo," the authors wrote, "is fundamental for our understanding of human development and to advance stem-cell-based regenerative approaches. (genengnews.com)
Mechanisms1
We demonstrate several mechanisms for molecular imaging, including intrinsically expressed GFP fluorescence, autofluorescence from Flavin proteins, and exogenous antibody-conjugated dyes. (nature.com)
Gene regulat1
The foxa gene is an integral component of the endoderm specification subcircuit of the endomesoderm gene regulatory network in the Strongylocentrotus purpuratus embryo. (biologists.com)
Experiments in which blastomere transplantation is combined with foxa MASO treatment demonstrate that, in the normal endoderm, a crucial role of Foxa is to repress gcm expression in response to a Notch signal, and hence to repress mesodermal fate. (biologists.com)
Developmental1
Our work provides the method and integrated multiomic resource for the investigation of disease-relevant loci and gene regulatory networks and their role in developmental defects affecting the pharyngeal endoderm and its derivatives. (bvsalud.org)
Human1
However, recent work also shows that key molecular aspects of human END regulation differ even from rodents. (bvsalud.org)
Development3
Despite the disease-relevance of pharyngeal endoderm, we still lack a comprehensive and integrative view of the molecular basis and gene regulatory networks driving pharyngeal endoderm development. (bvsalud.org)
Studies in vertebrates have outlined conserved molecular control of definitive endoderm (END) development. (bvsalud.org)
Brain development is a complex process achieved in a remarkably controlled spatial and temporal manner through coordinated cellular and molecular events. (ehu.eus)
Multi-modal three dimensional (3D) optical imaging combining both structural sensitivity and molecular specificity is highly desirable in biomedical research. (nature.com)
In this paper, we present a method termed oblique scanning laser microscopy (OSLM) to combine optical coherence tomography (OCT), for simultaneously volumetric structural and molecular imaging with cellular resolution in all three dimensions. (nature.com)
However, this molecular specificity comes with a dilemma that the structural context and other unspecified molecules would not appear in the images, i.e . we can only see what we choose to see. (nature.com)
Therefore, there is an increasing need for multimodal imaging techniques that can provide both molecular specificity and the structural context. (nature.com)
Despite a large body of literatures and long standing efforts on this subject, it is still challenging to provide a multimodal system that can achieve volumetric structural and molecular imaging simultaneously with equivalent high resolutions for both modalities. (nature.com)
In addition to the fluorescence mode for molecular specificity, LSM can simultaneously detect the backscattered light in the reflectance mode which is sensitive to structural heterogeneity 2 , 3 . (nature.com)
Regulatory2
The analysis of gene expression changes is the first level of exploration for any regulatory at the molecular and cellular levels [4]. (gene-quantification.org)
The identification of gene regulatory networks (GRNs) facilitates the understanding of the underlying molecular mechanism of various biological processes and complex diseases. (biomedcentral.com)
Targets1
The complex formed produces or releases second messengers that integrate and adapt the signal, amplifying it, by activating molecular targets, which in turn trigger effectors that will lead to the desired cellular response. (wikipedia.org)
Earliest1
The earliest definitive account was given by Swiss scientist Albert von Kölliker in 1849. (wikipedia.org)
Role1
Thus, the use of biomarker signatures may play an important or even 'a definitive role in developing personalized medical health care. (gene-quantification.org)