To overcome this substrate utilization defect, a predicted glcG gene, encoding enzyme II of the D-glucose phosphoenolpyruvate-dependent phosphotransferase system (PTS), was first disrupted in the ABE-producing model strain Clostridium acetobutylicum ATCC 824, resulting in greatly improved D-xylose and L-arabinose consumption in the presence of D-glucose. (hw.ac.uk)
The integrated model was able to successfully reproduce ABE fermentations of the WT C. acetobutylicum (ATCC 824), as well as its mutants, using data obtained from our own experiments and from literature. (illinois.edu)
It has the ability to convert carbohydrates to butyrate, acetate, carbon dioxide, and molecular hydrogen through fermentation. (wikipedia.org)
Shukla, R, Kang, W & Sirkar, KK 1988, ' Toxicity of organic solvents to clostridium acetobutylicum for extractive ABE fermentation - Scientific note ', Applied Biochemistry and Biotechnology , vol. 18, no. 1, pp. 315-324. (njit.edu)
One canonical example of such processes is acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum, during which cells convert carbon sources to organic acids that are later reassimilated to produce solvents as a strategy for cellular survival. (illinois.edu)
More than a century ago, in 1915, a senior lecturer in biochemistry at the University of Manchester named Dr. Chaim Weizmann invented a fermentation process that converted starch - a poly-sugar readily available from corn and potatoes - into acetone and butyl alcohol, facilitated by a bacteria, Clostridium acetobutylicum, that Dr. Weizmann had previously isolated. (weizmann-usa.org)
Here we demonstrate the formation of an artificial consortium between two anaerobic bacteria, Clostridium acetobutylicum (Gram-positive) and Desulfovibrio vulgaris Hildenborough (Gram-negative, sulfate-reducing) in which physical interactions between the two partners induce emergent properties. (hal.science)
In other research projects, he explores physiology and gene regulation in biotechnologically relevant bacteria like different Bacillus species, Gluconobacter oxydans, and solventogenic Clostridia, and furthermore the biosynthesis of hydrocarbons and natural gene transfer in high-GC Gram-positive bacteria like Micrococcus luteus. (tum.de)
This property is considered unusual when compared to other cohesin-dockerin interactions and is unique, thus far, to C. acetobutylicum bacteria. (phys.org)
Butanol1
The Gram-positive anaerobic bacterium Clostridium acetobutylicum, known for its excellent capability of producing ABE (acetone, butanol, and ethanol) solvent, is limited in using lignocellulose because of inefficient pentose consumption when fermenting sugar mixtures. (hw.ac.uk)
J ournal of Molecular Microbiology and Biotechnology . (tum.de)
Bacterium3
Clostridium pasteurianum (previously known as Clostridium pastorianum) is a bacterium discovered in 1890 by the Russian microbiologist Sergei Winogradsky. (wikipedia.org)
Clostridium acetobutylicum is an anaerobic, spore-forming bacterium with the ability to ferment starch and sugars into solvents. (igem.org)
The latest discovery, on the cellulosome assembly of the bacterium Clostridium acetobutylicum, takes this prospect further by switching between two functional sites, rather than simply on or off. (phys.org)
Molecular biology has allowed a detailed understanding of genes and enzymes, required for solventogenesis. (igem.org)
Metabolic1
however, little is known about how pentose sugars are catabolized through the metabolic pathways in clostridia. (elsevierpure.com)
Thermocellum1
Much of our understanding of its catalytic components, architecture, and mechanisms of attachment to the bacterial cell and to cellulose, has been derived from the study of Clostridium thermocellum [ 1 , 2 , 3 , 4 ]. (cazypedia.org)
Pasteurianum3
Clostridium pasteurianum is a producer of carboxylic acids. (wikipedia.org)
however, it is still unclear whether the growth parameters which have been shown to produce favorable solvent production in C. acetobutylicum played a significant role in the regulation of metabolism in C. pasteurianum in a similar fashion. (wikipedia.org)
Clostridium pasteurianum is a large, Gram-positive, spore-forming bacillus. (wikipedia.org)
Glucose1
At the molecular level, it was found that replacement of glucose by starch has enhanced the relative expression level of ccpA encoding catabolite control protein A. Therefore, the repression of starch utilization by glucose could be probably relieved. (hindawi.com)
Functional1
on genome sequence should be carefully addressed, as observed in Clostridium acetobutylicum where both functional oxidative and reductive TCA cycles were confirmed experimentally in contrast to the previous genomic interpretation [15]. (screeninglibraries.com)
Pathway2
The phosphoketolase pathway was found to be active, which contributed up to 40% of the xylose catabolic flux in C. acetobutylicum. (elsevierpure.com)
To our knowledge, this is the first time that the in vivo activity of the phosphoketolase pathway in clostridia has been revealed. (elsevierpure.com)
Synthetic1
A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing. (jefferson.edu)
Acetate1
The phosphoketolase was overexpressed in C. acetobutylicum, which resulted in slightly increased xylose consumption rates during the exponential growth phase and a high level of acetate accumulation. (elsevierpure.com)
Advances in computational methods to infer ancestral sequences, and to estimate patterns of selective constraint, can be used to infer hypotheses of molecular adaptation that can then be tested in the laboratory. (usherbrooke.ca)
Trizzino M, Audisio P, Antonini G, De Biase A, Mancini E. Comparative analysis of sequences and secondary structures of the rRNA internal transcribed spacer 2 (ITS2) in pollen beetles of the subfamily Meligethinae (Coleoptera, Nitidulidae): potential use of slippage-derived sequences in molecular systematics. (jefferson.edu)
Cellular2
Molecular and cellular approaches show that tight cell-cell interactions are associated with an exchange of molecules, including proteins, which allows the growth of one partner (D. vulgaris) in spite of the shortage of nutrients. (hal.science)
Microalgae can divert cellular resources towards lipid accumulation under conditions of stress, and identifying the molecular trigger for this response could aid in engineering improved lipid productivity, a precursor for biofuel production. (biomedcentral.com)
Method1
I'm an experimentalist 'Pre-doc' (I still have to finish my PhD thesis) and my work on the COSMIC project will focus on setting up a metabolomic analysis method for Clostridium acetobutylicum. (fairdomhub.org)
University1
Prof. Alon Chen, President of the Weizmann Institute of Science, has been awarded the 2022 Bennett Family Award for Collaboration in the Science of Wellness, along with Juan de Pablo, the Executive Vice President for Science, Innovation, National Laboratories, and Global Initiatives, and the Liew Family Professor in Molecular Engineering at the Pritzker School of Molecular Engineering at the University of Chicago. (weizmann-usa.org)
Environments1
Anaerobes are organisms that can live and grow in environments devoid of molecular oxygen (O2). (alliedacademies.org)
Activity1
A phosphoketolase from C. acetobutylicum was purified and characterized, and its activity with xylulose-5-P was verified. (elsevierpure.com)
Model1
During the model construction, a bottom-up approach was used to link molecular events at the singlecell level into the events at the population level. (illinois.edu)
Level1
How is this achieved at the molecular level? (usherbrooke.ca)
Journal1
Journal of Physics B: Atomic, Molecular and Optical Physics. (princeton.edu)
Events1
The process by which a chemical or physical signal is transmitted through a cell as a series of molecular events is called signal transduction. (screeninglibraries.com)