Molecular clone cell lines genetically clone cells. Medical search. Frequent questions

Demonstrated experience in mouse handling and animal care, along with expertise in microbiology and molecular biology techniques, will play a pivotal role in conducting our research. (hospitalcareers.com)
Since SLAMF receptors play a role in NK cell biology, we reasoned that these receptors may impact NK cell-mediated CLL immunity. (nature.com)
The budding yeast Saccharomyces cerevisiae has long been an outstanding platform for understanding the biology of eukaryotic cells. (microbialcell.com)
Robust genetics, cell biology, molecular biology, and biochemistry complement deep and detailed genome annotation, a multitude of genome-scale strain collections for functional genomics, and substantial gene conservation with Metazoa to comprise a powerful model for modern biological research. (microbialcell.com)
By the end of this article, you may have learned a lot about the molecular biology of viruses. (thebulletin.org)
iPSCs "can be turned into disease-relevant cells such as nerve cells, allowing researchers to study disease biology in the very types of cells that become affected. (jax.org)
Before embarking on a def-inition of systems biology, it may be worth remember-ing that confusion and controversy surrounded the in-troduction of the term "molecular biology," with claims that it hardly differed from biochemistry. (pharmaceuticalintelligence.com)
Yet in retro-spect molecular biology was new and different. (pharmaceuticalintelligence.com)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

Most human embryos reproduced by most human cloning techniques would actually be genetically unique -- i.e., having never existed before. (lifeissues.net)
These procedures are likely to lead to an increase in international trafficking of human cells, eggs and embryos. (i-sis.org.uk)
When combined with normal pre-implantation embryos and transferred to foster mothers, ES cells contribute to the development of many different tissues, including the germline. (cshlpress.com)

Beyond these clinical applications, cloning and related molecular techniques are used to obtain basic information about how genes are put together and regulated. (biologywriter.com)
Treating colon cancer cells with a DNA methyltransferase inhibitor, 5-aza-2'-deoxycytidine (DAC), led to elevated expression levels of CYP1B1 and CYP3A4 through demethylation of the promoter regions of related genes. (bvsalud.org)
DEP increased colon cancer cell sensitivity to 5-FU in association with increased expressions of tumor-suppressor p21 and major histocompatibility complex class II genes. (bvsalud.org)
B.A. Osborne, S.W. Smith, Z.-G. Liu, K.A. McLaughlin, and L.M. Schwartz, Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes. (nhbs.com)
In this study, we employed a genome-wide CRISPR-Cas9 loss-of-function screen based on cell survival in order to identify genes implicated in the host response to dsRNA. (cnrs.fr)
Using a genome-wide screening approach, we set to identify genes involved in dsRNA entry, sensing, and apoptosis induction in human cells. (cnrs.fr)
We have shown an association of ER stress with disease pathology using cell culture and in-house generated knock-in mouse models of chondrodysplasia caused by mutations in cartilage ECM genes such as collagen X, COMP and matrilin 3. (manchester.ac.uk)
In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. (cyberleninka.org)
In fact, this was shown to be possible by the transfection of genes into GS cells (Kanatsu-Shinohara et al. (cyberleninka.org)
The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1). (lookformedical.com)
Thanks to human induced pluripotent stem cell (iPSC) and gene-editing technologies, it's possible to derive every kind of brain cell type, insert dementia -related genes and study them in culture. (jax.org)
Uncontrolled cell proliferation is the hallmark of cancer, and genes that directly regulate the cell cycle are typically damaged in tumor cells ( 10 ). (jcancer.org)
Embryonic stem (ES) cells are important vehicles for transfer of genes into mice. (cshlpress.com)
When these cells are genetically manipulated, this approach discloses much about the function of specific genes. (cshlpress.com)
The precise pathophysiology of inherited single cell and multilineage cytopenias has not been elucidated despite the identification of many of the genes mutated in these disorders. (medscape.com)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
Recent advances have been made in the of host factors, polymorphisms, and candidate genes associated characterization of the immune response to low-molecular- with occupational asthma may improve our understanding of weight agents. (cdc.gov)

Dominant white (W) is a group of genetically related coat color alleles on the KIT gene of the horse, best known for producing an all-white coat, but also able to produce various forms of white spotting, as well as bold white markings. (wikipedia.org)
It has been found that even within a group of genetically similar cells, small subgroups of resistant cells emerge. (cbirt.net)

Our current efforts are focused on genetically dissecting out the roles of different ER stress pathways in disease pathogenesis using a series of conditional ER stress-related mouse lines crossed with our own chondrodysplasia lines. (manchester.ac.uk)
Distinct pathways of cell migration and antiapoptotic response to epithelial injury: structure-function analysis of human intestinal trefoil factor. (embl.de)
Animal models of polycystic kidney disease (PKD) have been invaluable in discovering and dissecting cellular and molecular pathways by which polycystic kidneys develop and by which progression of all types of PKD may be modified [ 1 ],[ 2 ]. (biomedcentral.com)
Cookson, a cell biologist who studies the underlying pathways that lead to Parkinson's disease and related disorders, explains that a typical iPSC project would involve reverting a cell line from a patient with a given mutation back to the wild-type (or "normal") sequence, to compare the two. (jax.org)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

Many of these accurate definitions can also be used in bills and treaties concerning related issues, e.g., human embryonic stem cell research, human genetic engineering, abortion, the use of abortifacients, conscience clauses, IVF and other artificial reproductive technology research and regulation, etc. (lifeissues.net)
Most other genetic engineering approaches have used the same general strategy, bringing the gene of interest into the target cell by first incorporating it into a plasmid or an infective virus. (biologywriter.com)
Furthermore, because of the antigenic and genetic diversity of 'Norwalk-like viruses'* (NLVs) and the inability to cultivate these viruses in cell lines, developing assays to detect the full spectrum of NLVs associated with outbreaks of gastroenteritis was not possible. (cdc.gov)
The resulting clones were plated on Petri dishes for propagation and a further genetic analysis. (eco-vector.com)
Clone 126-2F4 was found out carrying the necessary genetic modifications. (eco-vector.com)
Selexis is able to modulate expression at the molecular scale and their strategy is to individually clone each polypeptic chain constituting a bi-specific antibody into an individual vector containing Selexis genetic elements. (cellculturedish.com)
Genetic engineering techniques were used to modify the human IL-2 gene, and the resulting expression clone encodes a modified human IL-2. (sydlabs.com)
TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure. (cyberleninka.org)
2004). In order to make GS cells more widely applicable for the study of spermatogenesis at the genetic and molecular levels, it is desirable to be able to modify their genome without disturbing their spermato-genic ability. (cyberleninka.org)
While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
In particular, hiPSC-based models provide a powerful tool to identify genetic risk factors, and to study cellular and molecular mechanisms that contribute to the pathogenesis of a disease. (frontiersin.org)
However, to realize the full potential of hiPSCs for disease modeling and drug screening, several challenges still need to be overcome, as there is the high variability between hiPSC lines, the risk of accumulating genetic aberrations when culturing hiPSCs, and the lack of standardized procedures for hiPSC generation as such (reviewed by Volpato and Webber, 2020 ). (frontiersin.org)
Interestingly, no genetic foundation for cell diversity was observed within resistant clones. (cbirt.net)
By isolating and reprogramming brain cells with dementia-causing genetic mutations, a team at JAX offers a powerful new research tool. (jax.org)
However, the process of making iPSCs from patients with these diseases, and the subsequent genetic engineering of the cell lines, is "difficult, expensive and time consuming," says Ward. (jax.org)
Like compounds from biological sources and low-molecular-weight nonoccupational asthma, occupational asthma is probably the chemicals cause occupational asthma after a latent period of result of multiple genetic, environmental, and behavioral exposure. (cdc.gov)

The rapid expansion in biomedical research using live-cell imaging techniques over the past several years has been fueled by a combination of events that include dramatic advances in spinning disk confocal microscopy instrumentation coupled with the introduction of novel ultra-sensitive detectors and continued improvements in the performance of genetically-encoded fluorescent proteins. (fsu.edu)
The libraries consist of genetically modified CHO-M lines that co-express helper proteins, which target the specific expression pathway at the origin of the pro-expression. (cellculturedish.com)
2009). Generation of Human Induced Pluripotent Stem Cells by Direct Delivery of Reprogramming Proteins. (igem.org)

Ray's lab was the first to clone mammalian collagen X sequences and went on to identify chondrodysplasias associated with mutations in collagen X. In 1999, Ray was awarded a Wellcome Trust Research Leave Fellowship to learn and apply mouse gene targeting techniques. (manchester.ac.uk)
CD133+ hunting machines will be created by genetically engineering microglial cells (BV-2) with two constructs using mammalian expression vectors. (igem.org)
Nek1 is a mammalian ortholog of the fungal kinase NIMA (never in mitosis A in Aspergillus nidulans ), which functions in DNA damage responses, regulates G2-M phase progression, and helps keep chromosome transmission to daughter cells faithful [ 5 ]-[ 10 ]. (biomedcentral.com)
Spermatogonial stem cells (SSCs) are pivotal for maintaining the lifelong sperm production of mammalian males. (cyberleninka.org)

B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)
Molecular diagnostics now enables detecting viruses in clinical and environmental specimens, linking of NLV strains causing outbreaks in multiple geographic locations, and tracing them to their sources in contaminated food or water. (cdc.gov)
The techniques described facilitate both understanding and regulation of cell death, promising to have immense value in the clinical treatment of cancer. (nhbs.com)
Chimeric antigen receptor T (CART) cell therapy targeting the B cell specific differentiation antigen CD19 has shown clinical efficacy in a subset of relapsed/refractory (r/r) diffuse large B cell lymphoma (DLBCL) patients. (frontiersin.org)
Blood cell and serum markers, along with clinical data of DLBCL patients who were scheduled for CART cell therapy were evaluated to search for biomarkers predicting CART cell responsiveness. (frontiersin.org)
Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. (lookformedical.com)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

In turn, they also have learned that massive cell death does not always represent pathology, but rather a normal and necessary "weeding out" in the development and life of an organism. (nhbs.com)
However, patient-derived cells may be more prone to develop disease-associated pathology after grafting. (lu.se)
Lastly, we evaluated the strategy of knocking out a-syn as a means to protect the cells from transfer of pathology upon grafting. (lu.se)

Expression is particularly high in the pancreas where it is thought to play a role in the differentiation of pancreatic beta cells. (embl.de)
In this study, the NTERA2-D1 (NT2) cells were induced towards differentiation (NT2-RA) with 10-5 M retinoic acid (RA) for three days and then subjected to various amounts of vitreous humor (VH). (bvsalud.org)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)

An inexpensive way to produce interferon was needed, and introducing the gene responsible for its production into a bacterial cell made that possible. (biologywriter.com)
The cell that had acquired the human interferon gene proceeded to produce interferon at a rapid rate, and to grow and divide. (biologywriter.com)
Soon there were millions of interferon-producing bacteria in the culture, all of them descendants of the cell that had originally received the human interferon gene. (biologywriter.com)
The human insulin gene has also been cloned in bacteria, and now large amounts of insulin, a hormone essential for treating some forms of diabetes, can be manufactured at relatively little expense. (biologywriter.com)
In the interferon experiment, a piece of DNA carrying the interferon gene was inserted into a plasmid, which then carried the gene into a bacterial cell. (biologywriter.com)
Since commencing my role as a professor in a newly established Department of Pharmacodynamics and Molecular Genetics at the School of Pharmacy, Iwate Medical University, on April 1, 2007, my research has focused on modifying gene expression of cytochrome P-450 (CYP) in established human colon cancer cells. (bvsalud.org)
Another facet of my research is centered around understanding the gene regulatory mechanisms of the CYP1 family through aryl hydrocarbon receptors (AhR)s under glucose-deprivation stress and in three-dimensional (3D) culture systems of human solid tumor cells. (bvsalud.org)
Because of breakthroughs such as the "switching" mechanism of the p53 tumor suppressor gene, as well as many technical innovations, the number of investigators of cell death has rapidly expanded over the last few years. (nhbs.com)
To create mouse stem cells with a conditional knockout of the humanized Snca gene, a previously obtained clone with the first Snca exon flanked by LoxP sites, was used. (eco-vector.com)
As a result of the study, a strategy for CRISPR/Cas9-assisted homologous recombination in the genome of mouse embryonic stem cells has been developed to create a fully humanized Snca gene encoding α-synuclein, and the clone genome of mouse embryonic stem cells has been edited using a CRISPR technology. (eco-vector.com)
Among the obligately intracellular gram-negative bacteria, a genetically related set is classified among the Protobacteria of the subgroup on the basis of sequence analysis of the 16S rRNA gene ( 1 , 2 , 8 , 9 ). (cdc.gov)
First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)
We have identified a novel gene, gtcA , involved in the decoration of cell wall teichoic acid of Listeria monocytogenes serotype 4b with galactose and glucose. (asm.org)
Your gene sequences and cells may be patented and sold on the open market without your ever knowing about it. (i-sis.org.uk)
Expression of OCT4 gene dropped after RA treatment, but it was recovered in NT2-RA-VH cells. (bvsalud.org)
It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. (sydlabs.com)
2006). Covalent Attachment of Low Molecular Weight Poly(ethylene imine) Improves Tat Peptide Mediated Gene Delivery. (igem.org)
Although we can connect structure to the gene, we can no longer infer its larger purpose in the cell or in the organism. (pharmaceuticalintelligence.com)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

The developed cell clone can serve to create a line of genetically modified mice that serve as a test system for pathophysiological and neuropharmacological studies associated with synucleinopathies. (eco-vector.com)
The use of genetically engineered mice with both gain-of-function and loss-of-function mutations has been particularly informative about the normal and pathophysiological actions of a number of regulatory peptides of the gastrointestinal tract. (embl.de)
Kidney development in kat2J homozygous mice is aberrant early, before the appearance of gross cysts: developing cortical zones are thin, populated by immature glomeruli, and characterized by excessive apoptosis of several cell types. (biomedcentral.com)
Late in life, kat2J heterozygous mice form renal cysts and the cells lining these cysts lack staining for Nek1. (biomedcentral.com)
The primary cilia of cells lining cysts in kat2J homozygous mice are morphologically diverse: in some cells they are unusually long and in others there are multiple cilia of varying lengths. (biomedcentral.com)
Tissue or cell-specific expression of the Nek1 protein was never reported, since no anti-Nek1 antibodies were available then, and the association of Nek1 with pleiotropic abnormalities including PKD was not made in mice until later [ 4 ]. (biomedcentral.com)
These data demonstrate that MAP3K4-dependent signalling events are required for normal expression of Sry during testis development, and create a novel entry point into the molecular and cellular mechanisms underlying sex determination in mice and disorders of sexual development in humans. (plos.org)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)

2009). Isolation and characterization of cancer stem like cells in human glioblastoma cell lines. (igem.org)
Purification, characterization and molecular cloning of alpha-2-macroglobulin in cobia, Rachycentron canadum . (springer.com)

1989). Immortalization of murine microglial cells by a v-raf/ v-myc carrying retrovirus. (igem.org)
Nek1 is detectable in all murine tissues but its expression in wild type and kat2J heterozygous kidneys decrease as the kidneys mature, especially in tubular epithelial cells. (biomedcentral.com)

Moreover, we describe experiments showing the presence of activated MKK4, a direct target of MAP3K4, and activated p38 in the coelomic region of the XY gonad at 11.5 d post coitum, establishing a link between MAPK signalling in proliferating gonadal somatic cells and regulation of Sry expression. (plos.org)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
BML mutations thus result in defects in DNA repair and genomic instability in the somatic cells, predisposing the patients to cancer development. (medscape.com)

A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J. Mogil, W.K. Nishioka, and D.R. Green, The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro. (nhbs.com)
The aim of the study was to create a genetically modified clone of mouse stem cells with a conditional knockout of humanized α-synuclein, which can be used for the reinjection into mouse blastocysts, as well as for basic and applied in vitro research in the field of pathophysiology and neuropharmacology. (eco-vector.com)
In vitro , CD3 + CD8 + CD27 - CD28 - compared to CD3 + CD8 + CD27 + CD28 + CART cells displayed similar CD19 + target cell-specific cytotoxicity, but were hypoproliferative and produced less cytotoxic cytokines (IFN-γ and TNF-α). (frontiersin.org)
2009). Physiologic Oxygen Concentration Enhances the Stem-Like Properties of CD133+ Human Glioblastoma Cells In Vitro. (igem.org)
Cells grown in vitro from neoplastic tissue. (lookformedical.com)

Students of apoptosis and other types of cell death hypothesize that virtually all cells are genetically programmed to self-destruct. (nhbs.com)
S.W. Sherwood and R.T. Schimke, Cell Cycle Analysis of Apoptosis Using Flow Cytometry. (nhbs.com)
When cells are deficient in NIMA or in certain NIMA-related kinases, they undergo apoptosis because of major errors in chromosome segregation. (biomedcentral.com)
Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)
Accelerated hematopoietic cell apoptosis has been demonstrated in virtually all IBMFS. (medscape.com)

But even this Bill of Rights may be inadequate to cope with rapid developments further down the line, such as human cloning, cell and tissue replacement and embryonic stem cell techniques. (i-sis.org.uk)
In my opinion there is no question but that the scientific information on stem cell research included in this science text book being used in Illinois schools incorporates some inaccurate scientific facts, and seems to be very partial to the use of human embryonic "stem cell" research. (lifeissues.net)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

White is both genetically and visually distinct from gray and cremello. (wikipedia.org)
In addition, the NII561-2000 virus was genetically distinct from HPeV-4 and HPeV-5. (cdc.gov)
In B cells, this class of receptors has been shown to be expressed in distinct patterns associated with development and activation [ 11 ]. (nature.com)
Here we report that Nek1 is expressed most strongly in the distinct subset of renal epithelial cells. (biomedcentral.com)

Indeed, our experiments showed significantly decreased degranulation capacity of primary NK cells from CLL patients expressing low levels of SLAMF1 and SLAMF7. (nature.com)
In certain outbreaks, the detection of a genetically identical strain of NLV among patients from different geographic locations provided substantial evidence to support the link among the cases that was indicated by epidemiologic observations. (cdc.gov)
Background: The widespread adoption of Allogeneic Hematopoietic Stem Cell Transplantation (Allo-HSCT) has significantly improved the survival rates of patients with hematological malignancies. (bvsalud.org)
Compared to healthy controls (n=24), DLBCL patients (n=33) showed significant lymphopenia, due to low CD3 + CD4 + T helper and CD3 - CD56 + NK cell counts, while cytotoxic CD3 + CD8 + T cell counts were similar. (frontiersin.org)
Twenty-six patients were infused with CART cells (median 81 days after leukapheresis) and were analyzed for the overall response (OR) 3 months later. (frontiersin.org)
Low frequency of differentiated CD3 + CD27 - CD28 - T cells at leukapheresis represents a novel pre-infusion blood biomarker predicting a favorable response to CART cell treatment in r/r DLBCL patients. (frontiersin.org)
2007). Adoptive Transfer of Chimeric Antigen Receptor Re-directed Cytolytic T Lymphocyte Clones in Patients with Neuroblastoma. (igem.org)
[3] Today, patients take insulin shots and pharmaceuticals to control their diabetes, and adult stem cell transplants have shown success at ameliorating the condition. (lozierinstitute.org)
A promising new approach to finding effective treatments is to study human brain cells that carry mutations found in Alzheimer's patients. (jax.org)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

Harvested GS cell colonies showed a high targeting efficiency with both TALEN and CRISPR/Cas9. (cyberleninka.org)
And with the development of CRISPR/Cas9 techniques, it is now possible to genetically manipulate iPSCs so that the effects of disease-related mutations can be studied. (jax.org)

Unlike embryonic stem cells, iPSCs are derived from adult human cells. (jax.org)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)

The therapy administered over 52 cycles led to complete molecular and hematological remission and resulted in 5.3 years overall survival. (bvsalud.org)
Both diseases show phenotypical similarity and both demonstrate bone marrow failure, skeletal growth deficiency, short stature, and predisposition to hematological malignancies, although they are genetically unrelated. (medscape.com)

During the early 1990s, breakthroughs in cloning and sequencing of Norwalk virus and Southampton virus ( 15--18 ) led to the development of sensitive molecular assays (e.g., reverse transcription-polymerase chain reaction [RT-PCR]), nucleotide hybridization probes, and enzyme-linked immunosorbent assays (ELISA) that used baculovirus-expressed viral antigens ( 19--33 ). (cdc.gov)
Orangu ™ is a non-cytotoxic, sensitive, colorimetric assay for the determination of viable cell numbers in cell proliferation and cytotoxicity assays. (biolynx.ca)

We are looking for a motivated Staff Research Associate II to work on a variety of projects with the laboratory to identify critical molecular targets in malignant gliomas and develop novel, brain-penetrant small molecule therapeutics to treat these brain cancers. (hospitalcareers.com)
A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
Malignant lymphoma composed of large B lymphoid cells whose nuclear size can exceed normal macrophage nuclei, or more than twice the size of a normal lymphocyte. (lookformedical.com)
Malignant lymphoma in which the lymphomatous cells are clustered into identifiable nodules within the LYMPH NODES. (lookformedical.com)

We showed that COG4 knockout led to a decrease of extracellular HS that specifically affected dsRNA transfection efficiency and reduced viral production, which explains the increased cell survival of these mutants.IMPORTANCE When facing a viral infection, the organism has to put in place a number of defense mechanisms in order to clear the pathogen from the cell. (cnrs.fr)
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). (lookformedical.com)
Environmental factors that can detect specific IgE antibodies against most low-molecular- affect the initiation of occupational asthma include the intrinsic weight agents has resulted in a search for alternative or characteristics of causative agents as well as the influence of the complementary physiopathologic mechanisms leading to airway level and route of exposure at the workplace. (cdc.gov)

Following careful assessment of various culture conditions, we describe here the robust continuous cultivation of the blood stages of P. cynomolgi lines derived from the Berok strain. (nature.com)
Three P. cynomolgi lines were available to us: the Berok strain cryopreserved in 2003 from Aotus trivirgatus (initially isolated in 1964), the B strain ( P. cynomolgi bastianelli , initially isolated in 1959) and the M strain ( P. cynomolgi Mulligan strain first isolated in the 1930s). (nature.com)
Haploid yeast cells expressing a SARS-CoV-2 protein fused to a high affinity GFP binding protein (GPB) are mated to the yeast GFP strain library. (microbialcell.com)
P. berghei ANKA 2.34 is a cloned, gametocyte producer line of the ANKA strain (PubMed: PMID: 15137943). (pberghei.eu)

Overexpression of SLAMF1 or SLAMF7 in IGHV mutated CLL cell models resulted in reduced proliferation and impaired responses to BCR ligation, whereas the knockout of both receptors showed opposing effects and increased sensitivity toward inhibition of components of the BCR pathway. (nature.com)
The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line) and is typically 0.2-2 ng/ml. (sydlabs.com)

Fluorescent Protein Technology - It took over thirty years, and the advent of recombinant DNA as well as vastly improved molecular biological approaches to see the pioneering work of Osamu Shimomura developed into a useful tool for live-cell imaging by Doug Prasher and Martin Chalfie. (fsu.edu)
Herewith, before the induction of the Cre-dependent recombination, this line is a representative model for studying a biological role of mutant Snca . (eco-vector.com)

If they are isolating cells from single colonies grown in semi-solid media, they will provide statistics to demonstrate monoclonality and this requires two rounds. (cellculturedish.com)
Upon exposure to therapeutic drugs, resistant clones form resistant colonies and proliferate. (cbirt.net)

Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. (cyberleninka.org)
The cultured SSCs, named germline stem (GS) cells, are now useful in research on various aspects of spermatogenesis. (cyberleninka.org)

The first is during isolation of clones. (cellculturedish.com)
Here, we describe the StemCellFactory, an automated, modular platform covering the entire process of hiPSC production, ranging from adult human fibroblast expansion, Sendai virus-based reprogramming to automated isolation, and parallel expansion of hiPSC clones. (frontiersin.org)

A previous study demonstrated that SLAMF1, SLAMF2, and SLAMF7 receptors are rather downregulated on CLL cells as compared to their normal B cell counterpart [ 12 ], suggesting that high expression of these molecules may have detrimental (e.g., antiproliferative) effects in the CLL context. (nature.com)
In the 3D culture of human liver cancer cells, I found Pregnane X Receptor being implicated in the regulation of CYP1A2, which aligns with the in vivo mode of CYP1A2 expression. (bvsalud.org)
Furthermore, by generating novel transgenic lines in which elevated ER stress (caused by the expression of a non-secreted, ER stress-inducing protein) is targeted to relevant chondrocytes in vivo by use of the collagen X or II promoter, we have demonstrated the capability of ER stress to induce chondrodysplasia. (manchester.ac.uk)
Thus, there is a need for host expression cell lines comprising cell line and vector systems that ensure optimal transcription of the different chains in precise ratios to ensure the, translation, correct assembly and secretion of the bi-specific antibody in question. (cellculturedish.com)
In the embryonic kidney, Nek1 expression is most prominent in cells that will become podocytes and proximal tubules. (biomedcentral.com)
To introduce altered Drosophila Gap1 transgenes into flies, the sevenless ( sev ) enhancer/promoter cassette, an eye-specific promoter, was used to drive expression in cone cell precursors at appropriate levels. (sdbonline.org)
Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
Activated α 2 macroglobulin induces matrix metalloproteinase 9 expression by low-density lipoprotein receptor-related protein 1 through MAPK-ERK1/2 and NF-κB activation in macrophage-derived cell lines. (springer.com)
Expression profiles for individual cells are then produced with scRNA-seq. (cbirt.net)

Remarkably, DAC treatment also increased colon cancer cell sensitivity to SN-38, an active metabolite of CPT-11, through the suppression of the anti-apoptotic protein Bcl-2. (bvsalud.org)
L.T. Bemis, F.J. Geske, and R. Strange, Use of the Yeast Two-Hybrid System for Identifying the Cascade of Protein Interactions Resulting in Apoptotic Cell Death. (nhbs.com)
Growth defects that result are termed SPIs and indicate that the query protein in specific contexts disrupts the normal homeostasis of the cell [2] . (microbialcell.com)
I described how CHO cells have been a very successful workhorse in the manufacture of recombinant protein therapeutics and that CHO cell lines have been optimized over many years to provide the best productivity and product quality. (cellculturedish.com)
Yemi explained that stable production of a recombinant protein in any cell line requires efficient incorporation of the DNA encoding the protein into the reproductive machinery of the cell (most frequently the chromosomes in the nuclear matrix) in a manner that allows for efficient transcription, translation and secretion from the cell. (cellculturedish.com)
Furthermore, owing to the foreign nature of the protein, the translation and secretory machinery of the host cell may not be adequately suited to the protein. (cellculturedish.com)
Recombinant Human interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. (sydlabs.com)
1997). Degradation of Amyloid β-Protein by a Metalloprotease Secreted by Microglia and Other Neural and Non-neural Cells. (igem.org)
The engineered BV-2s will be equipped to locate the specific GBMs and label the targeted cells with a tat-GFP fusion protein. (igem.org)
Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
PB000374.03.0), a malaria sporozoite protein critical for sporozoite motility and invasion into host cells. (pberghei.eu)
BLM encodes 1417 amino acids that code for a protein in the nuclear matrix of growing cells, which is a member of the RecQ family of helicases. (medscape.com)

Note that each and every individual "loophole" discussed below that permits human cloning by default (and most bills have literally dozens of such loopholes) thus permits it for both "therapeutic" and for "reproductive" human cloning. (lifeissues.net)
What is the function of the cells early in disease when therapeutic intervention would be most advantageous? (stanford.edu)
The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
A collaborative research effort led by scientists and engineers at the University of Pennsylvania and Northwestern University led to the development of FateMap, a framework that combines DNA barcoding, single-cell RNA sequencing (scRNA-seq), and computational analysis to study the 'fate' or outcomes after exposing cancer cells to therapeutic drugs. (cbirt.net)
Through a strategic integration process, these barcoded cells are subjected to therapeutic drug treatments, with a specific focus on this study's use of vemurafenib for targeted therapy. (cbirt.net)

P.J. Fraker, L.E. King, D. Lill-Elghanian, and W.G. Telford, Quantification of Apoptotic Events in Pure and Heterogeneous Populations of Cells using the Flow Cytometer. (nhbs.com)
Despite this heterogeneous response, blood pre-infusion biomarkers predicting responsiveness to CART cell therapy are currently understudied. (frontiersin.org)

This procedure of producing a line of genetically identical cells from a single altered cell, called cloning, made every cell in the culture a miniature factory for producing interferon. (biologywriter.com)

This experiment first connected the genotype directly to the phenotype on a molecular level, although efforts in that direction can certainly be found in the work of Archibald Garrod, Sewell Wright, and others. (pharmaceuticalintelligence.com)

Ray Boot-Handford is a Professor of Biochemistry within the Wellcome Trust Centre for Cell-Matrix Research. (manchester.ac.uk)

An approach to obtaining clones of mouse genetically modified stem cells expressing pathological humanized α-synuclein, has been proposed and implemented. (eco-vector.com)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

In the final stage, we will then hopefully be able to modify the system accordingly, to serve medical purposes: identification and elimination of CD133+ cells and hence contributing towards preventing the fatal consequences of Glioblastoma multiforme. (igem.org)

[ 7 , 8 ] Sister chromatid exchanges are considered a sensitive indicator for cell genome instability, as they are thought to be the outcome of DNA double-strand breaks resulting from homologous recombination repair. (medscape.com)

Specific duties include tissue cell culture, flow cytometry, PCR, western blots, immunofluorescence, animal work, preparation of data for publication and grant proposals, ordering of supplies and equipment, upkeep of equipment and general lab maintenance. (hospitalcareers.com)
[5] Conditions such as anemias and immunodeficiencies, for which fetal tissue attempts largely failed, are now treated routinely with adult stem cells, including umbilical cord blood stem cells, [6] even while the patient is still in the womb. (lozierinstitute.org)

So when I was recently contacted by an earnest and amiable member of a local school board who was concerned about the questionable manner in which the issue of "stem cell" research - both human embryonic and adult - was presented to the high school students in his district in a currently-used science textbook, I agreed to evaluate that section in the text for him. (lifeissues.net)
My edited analysis of the section on "stem cell research" in this science textbook is copied below. (lifeissues.net)
Cell Stem Cell, 4, 472-476. (igem.org)
Ward was on a research team that achieved the first successful merger of stem cell-derived cell types and CRISPR screening technologies. (jax.org)
Inherited bone marrow failure syndromes (IBMFS) are usually the result of intrinsic stem cell/progenitor defects. (medscape.com)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)

They are known to be involved in the regulation of NK and T cell responses, mostly by homotypic interactions except for SLAMF2 (CD48) and SLAMF4 that interact with each other [ 10 ]. (nature.com)

Surely one of the most critical issues before the world today is the issue of human cloning. (lifeissues.net)
The "pros" and "cons" of human cloning research have already been dealt with at length in the literature, so they will not be reviewed here. (lifeissues.net)
Rather, after having published analyses of dozens of state, national, federal and international legislative attempts to ban human cloning research, I simply wish to offer seriously considered suggestions for the use of scientifically accurate language and definitions to be used in such endeavors in order to prevent loopholes which would result in much human cloning not being really banned. (lifeissues.net)
Thus, e.g., leaving out certain human cloning techniques in a bill means that the bill will not ban the use of those forms of human cloning techniques -- and thus they will be allowed. (lifeissues.net)
Thus to use the phrase "of an existing or previously existing human being" to refer to the product of human cloning would not be a scientifically accurate description of the cloned or genetically engineered human embryo -- thus creating yet another loophole in the bill or treaty. (lifeissues.net)
Furthermore, the administration of DAC and the histone deacetylase inhibitor depsipeptide [(DEP), an anti-cancer drug romidepsin] significantly increased the cellular sensitivities of human colon cancer cells to CPT-11 and 5-FU, respectively. (bvsalud.org)
By challenging HCT116 human cells with either synthetic dsRNA or Sindbis virus (SINV), we identified the heparan sulfate (HS) pathway as a crucial factor for dsRNA entry, and we validated SINV dependency on HS. (cnrs.fr)
Interestingly, we uncovered a novel role for COG4, a component of the conserved oligomeric Golgi (COG) complex, as a factor involved in cell survival to both dsRNA and SINV in human cells. (cnrs.fr)
Each of these infectious diseases is designated by the major target cell: human monocytic ehrlichiosis is caused by Ehrlichia chaffeensis, and human granulocytic ehrlichiosis by an E. equi -like organism. (cdc.gov)
The taxonomic positions of these bacteria have been determined with much greater scientific clarity by the recent application of molecular methods, which were critical for the identification of E. chaffeensis and essential for that of human granulocytic ehrlichia (HGE) ( 1 , 2 ). (cdc.gov)
Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

Severine explained that they are able to demonstrate single cell origin from a single progenitor cell at various stages. (cellculturedish.com)

Using a diverse range of technical approaches and model systems, the chapters in this volume cover topics from the cellular and organismal to the molecular and anatomical. (nhbs.com)
It offers a cross-disciplinary look at cell death using a broad range of technical approaches and model systems. (nhbs.com)
Extreme difficulty in developing methods for cultivation of these ehrlichiae and, until recently, the lack of molecular approaches to the study of uncultivated organisms partly explain why these far from rare diseases were not detected sooner ( 3 - 5 ). (cdc.gov)

The second challenge is that the cell line development process must be redesigned to meet the unique characteristics of bi-specific antibodies. (cellculturedish.com)
This is a single cell line designed for difficult to produce bi-specific antibodies in final assembled format. (cellculturedish.com)

Diffuse large B cell lymphoma (DLBCL) represents the most frequent form of non-Hodgkin's lymphoma (NHL). (frontiersin.org)

Endogenous regulation of the cell cycle depends on phosphorylation and dephosphorylation of the cyclin- cyclin-dependent kinase (CDK)-cyclin-dependent kinase inhibitor (CDKI) pathway. (jcancer.org)

Clonal evolution of the leukemic clone could be demonstrated using DNA sequencing methods. (bvsalud.org)
FateMap helps identify resistant cells from subpopulations derived from single-cell (clonal) cancer populations and analyze their unique characteristics. (cbirt.net)

Cartilage has a single cell type, known as the chondrocyte, which secretes the abundant extracellular matrix in which these cells are embedded. (manchester.ac.uk)
Plant cells are surrounded by an extracellular matrix that consists mainly of polysaccharides. (mdpi.com)

CTRP plays a role in the motility of ookinetes and invasion of midgut epithelial cells. (pberghei.eu)

Among those molecular cues, double-stranded RNA (dsRNA) is a very potent inducer of different reactions at the cellular level that can ultimately lead to cell death. (cnrs.fr)

A classification of B-lymphocytes based on structurally or functionally different populations of cells. (lookformedical.com)

In this role, the Staff Research Associate I provides laboratory support under the direction of the Principal Investigator and a senior scientist in the Division of Molecular and Cellular Oncology. (hospitalcareers.com)

Detailed methods for using ES cells are described in the manual Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition . (cshlpress.com)

α-synuclein is one of the key molecular links in the pathogenesis of Parkinson's disease. (eco-vector.com)

At some point, they cause the bacterial cells to burst, releasing thousands more viruses. (biologywriter.com)
Through natural selection, some types of bacteria have acquired powerful weapons against these viruses: they contain enzymes called restriction endonucleases that fragment the viral DNA as soon as it enters the bacterial cell. (biologywriter.com)
Why don't restriction endonucleases cleave the bacterial cells' own DNA as well as that of the viruses? (biologywriter.com)
These results show that LMP1 , mour viruses classified by IARC in EBV-specific T-cell response. (who.int)

Int J Biochem Cell Biol. (embl.de)
J Cell Biochem 111, 607-617. (springer.com)

Detailed molecular analyzes showed that SLAMF1 and SLAMF7 receptors mediate their BCR pathway antagonistic effects via recruitment of prohibitin-2 (PHB2) thereby impairing its role in signal transduction downstream the IGHV-mutant IgM-BCR. (nature.com)

The barcodes for clones obtained from the previously performed steps in FateMap are amplified and, subsequently, sequenced to produce a cDNA library. (cbirt.net)

Coordinated localisation of mucins and trefoil peptides in the ulcer associated cell lineage and the gastrointestinal mucosa. (embl.de)
The ulcer associated cell lineage (UACL) occurs at sites of chronic ulceration in Crohn's disease, expresses all three trefoil peptides, and is implicated in mucosal restitution. (embl.de)

A relatively small number of cells, however, do have the capacity to give rise to different kinds of cells. (lifeissues.net)
High-molecular-weight relatively small proportion of exposed workers. (cdc.gov)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)

Finally, in this role you will perform animal and molecular techniques to support gnotobiotic research projects and provide technical support for the PI-run. (hospitalcareers.com)

Anatomy25

Organisms41

Diseases24

Chemicals and Drugs60

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes77

Disciplines and Occupations1

Information Science4

Biology9

Embryos3

Genes18

Group of genetically2

Pathways5

Genetic16

Proteins3

Mammalian4

Clinical9

Pathology3

Differentiation7

Gene20

Mice8

Characterization2

Murine2

Somatic3

Vitro5

Apoptosis5

Embryonic stem3

Distinct4

Patients11

CRISPR-Cas92

IPSCs3

Hematological2

Assays2

Malignant4

Mechanisms3

Strain4

Proliferation2

Biological2

Colonies2

Germline2

Isolation2

Expression9

Protein13

Therapeutic5

Heterogeneous2

Identical1

Phenotype1

Biochemistry1

Pathological2

Glioblastoma1

Genome1

Tissue2

Stem Cell6

Involved in the regulation1

Human14

Fibroblasts2

Progenitor cell1

Approaches3

Antibodies2

Form of non-Hodgkin'1

Regulation1

Clonal2

Extracellular matrix2

Epithelial1

Cues1

Populations of Cells1

Oncology1

Laboratory1

Pathogenesis1

Viruses4

Biochem2

Downstream1

CDNA1

Peptides2

Relatively small3

Techniques1