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  • proteins
  • A high reproducibility in gel pore size and a high-yield electroelution of proteins performed by this highly reliable technique strongly correlate with the polymerization time of the acrylamide gels representing an inherent stability constant of the separation system. (wikipedia.org)
  • Molecular evolution is the process of change in the sequence composition of cellular molecules such as DNA, RNA, and proteins across generations. (wikipedia.org)
  • After the 1970s, nucleic acid sequencing allowed molecular evolution to reach beyond proteins to highly conserved ribosomal RNA sequences, the foundation of a reconceptualization of the early history of life. (wikipedia.org)
  • Established in the late 1960s, Brookhaven Instruments pioneered modern techniques in characterizing nanoparticles, proteins, and polymers using light scattering techniques such as dynamic, static, electrophoretic, and phase analysis for: particle size, zeta potential, molecular mass and absolute molar mass analysis. (wikipedia.org)
  • It determines the absolute molecular weight of proteins and polymers. (wikipedia.org)
  • pore size
  • However, for buffer exchange and desalting applications, it is mainly the maximum effective pore size (exclusion limit or molecular weight cut off (MWCO) of the resin) that determines the size of molecules that can be separated. (wikipedia.org)
  • The pore size of a gel must be carefully controlled in order to be able to apply the gel to a given separation. (wikipedia.org)
  • enzyme
  • Physical entrapment of an enzyme in a gel lattice is an immobilization method in which no modification of the amino acid residues is needed, and which offers the advantage of reaction conditions usually so mild that few significant changes in the enzyme structure occur. (springer.com)
  • He isolated an inducible extracellular homogenous enzyme, which shows a 7.5-fold increases in its activity after DEAE cellulose column chromatography. (wikipedia.org)
  • affinity
  • Other desirable properties of the gel forming agent are the absence of ionizing groups and, in a given solvent, low affinity for the substances to be separated. (wikipedia.org)
  • mass
  • The molecular mass of the cytotoxin was estimated to be about 50 kDa. (scielo.br)
  • This gene is with an open reading frame of 2079 base pairs (bp), encoding a protein of 693 amino acids, corresponding to a molecular mass of 79569 Da. (wikipedia.org)
  • Disadvantages are, for example, that only a limited number of bands can be accommodated because the time scale of the chromatogram is short, and, in general, there must be a 10% difference in molecular mass to have a good resolution The technique was invented by Grant Henry Lathe and Colin R Ruthven, working at Queen Charlotte's Hospital, London. (wikipedia.org)
  • The EW is defined as the weight of Nafion (in terms of molecular mass) per sulfonic acid group. (wikipedia.org)
  • Its molecular weight is 25 kDa (from positions 19 - 239), which conforms to the molecular mass predicted from its cDNA sequences. (wikipedia.org)
  • Chromatography can be described as a mass transfer process involving adsorption. (wikipedia.org)
  • The use of low dispersity standards allows the user to correlate retention time with molecular mass, although the actual correlation is with the Hydrodynamic volume. (wikipedia.org)
  • 3000
  • Synthetic polyethylene wax having low molecular weight of 250 to 3000 and a density of 0.930 to 0.950 is prepared in a high pressure process at pressures of at least 800 bar using a carbonyl compound such as acetaldehyde as chain transfer agent. (freepatentsonline.com)
  • high molecular
  • Polyether polyols made from the catalysts contain reduced levels of high-molecular-weight (Mn greater than 400,000) components and consistently perform better in urethane applications such as flexible and molded foams. (google.es)
  • Traditionally, polyanhydrides have been prepared by melt condensation polymerization, which results in high molecular weight polymers. (wikipedia.org)
  • biologists
  • The advent of protein sequencing allowed molecular biologists to create phylogenies based on sequence comparison, and to use the differences between homologous sequences as a molecular clock to estimate the time since the last universal common ancestor. (wikipedia.org)
  • In the late 1960s, the neutral theory of molecular evolution provided a theoretical basis for the molecular clock, though both the clock and the neutral theory were controversial, since most evolutionary biologists held strongly to panselectionism, with natural selection as the only important cause of evolutionary change. (wikipedia.org)
  • composition
  • 2. A composition according to claim 1, wherein said inulin has a molecular weight greater than 8,000. (google.es)
  • 3. A composition according to claim 1, comprising particles of gamma inulin, wherein the gamma inulin has a molecular weight in the range of from about 8,000 to about 16,000 and is virtually insoluble in water at 37 C. (google.es)
  • spectroscopy
  • Digilab pioneered the world's first commercial FTIR spectrometer (Model FTS-14) in 1969 (Digilab FTIRs are now a part of Agilent technologies's molecular product line after it acquired spectroscopy business from Varian). (wikipedia.org)
  • differences
  • Using the mutation rate per generation and the number of nucleotide differences between two sequences, divergence times can be estimated effectively via the molecular clock. (wikipedia.org)
  • buffer
  • For typical desalting and buffer exchange applications choosing a resin with a molecular weight cut off between 5 and 10KDa is usually best. (wikipedia.org)
  • Sealed chromatography cartridges or columns work similarly except the sample and buffer is pumped into and through the resin by an external device such as a liquid chromatographic (LC) system, also requiring collection and monitoring of several fractions. (wikipedia.org)
  • known
  • The well-known Fischer-Tropsch process produces waxy products, but these materials tend to have a higher molecular weight "tail" than paraffin waxes which affects their properties. (freepatentsonline.com)
  • The lower wavelength limit was chosen to encompass the highest known vibration frequency due to a fundamental molecular vibration. (wikipedia.org)
  • product
  • The content and structure of a genome is the product of the molecular and population genetic forces which act upon that genome. (wikipedia.org)
  • assay
  • The FDA has approved four variations of the LAL test: gel-clot, turbidimetric, colorimetric, and chromogenic assay. (wikipedia.org)
  • solution
  • It is an example of a sol-gel process wherein a molecular precursor (typically tetraethylorthosilicate) is first reacted with water in an alcoholic solution, the resulting molecules then joining together to build larger structures. (wikipedia.org)