• The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses. (nih.gov)
  • The molecular characteristics of the Triton X-100 solubilized renal and mammary PRL receptors were assessed by gel filtration and by migration analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after affinity labeling of the binding sites with 125I-human growth hormone. (eurekamag.com)
  • The molecular weight of the native chicken erythrocyte catalase was estimated at 240 kDa by gel filtration. (tubitak.gov.tr)
  • The PFY-147-EPS was purified by gel-filtration chromatography. (bvsalud.org)
  • POMC IV was subjected to further purification on a Sephadex G-50 gel filtration column. (indexindex.com)
  • Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by Sephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. (ui.ac.id)
  • Gel filtration chromatography yielded an anomalously large Stokes radius, suggesting a fully unfolded conformation. (ox.ac.uk)
  • The type of HPLC selected is dependent on oligo sequence composition and characteristics. (idtdna.com)
  • Dual HPLC purification can be used to purify dual-labeled probes and Molecular Beacons bearing NHS ester modifications. (idtdna.com)
  • A nearly 136-fold enzyme purification was obtained from chicken erythrocyte by acetone precipitation, ethanol-chloroform treatment, CM-cellulose and Sephadex G-200 chromatography. (tubitak.gov.tr)
  • After separation and purification, the homogeneous polysaccharide was obtained with a molecular weight of 2.6 × 103 Da. (bvsalud.org)
  • The CHO cell line secretes recombinant factor IX into a defined cell culture medium, and the recombinant factor IX is purified by a four-step chromatography purification process that does not require a monoclonal antibody step. (globalrph.com)
  • However, molecular analyses of T1r have been hampered due to the difficulties in recombinant expression and protein purification, and thus little is known about mechanisms for taste perception. (nature.com)
  • Absorption characteristics were observed via spectroelectrochemical studies and comparable maximum absorption wavelengths, polaron and bipolaron band formations and color changes upon oxidation were reported from the results of absorption spectra of the polymers. (metu.edu.tr)
  • The sophisticated molecular design of these polymers guarantees a superior, stable lubricant that delivers consistent performance, whether you're starting your engine in the frosty depths of winter or operating at the peak of summer heat. (chempol.co.uk)
  • Gel permeationsize exclusion chromatography 5 chapter 2 gpcsec overview 6 polymers 6 size matters 6 how does gpcsec work 7 who uses gpcsec, what for and why 8 calibrations 8. (web.app)
  • Polymers are defined by the characteristics of their constituent monomer(s) and their microstructural arrangement. (spectra-analysis.com)
  • Polymers, Solutions, Gels and Phase Behavior. (lu.se)
  • In addition, the molecular function of some of the described proteins suggests a central role for SVEVs in the cytotoxicity of the snake venom and sheds new light in the envenomation process. (nature.com)
  • Students will investigate a range of subjects, including enzyme activity, characteristics, cell location, and the evolution of mammalian proteins, by following the step-by-step methods in their laboratory guides. (modernbio.com)
  • Protein fingerprinting is a popular method for this aim, in which the electrophoretic characteristics of specific proteins are examined in multiple species. (modernbio.com)
  • In this chapter, we will describe how zein proteins influence characteristics of maize grain and nutritional value of maize. (intechopen.com)
  • Proteins are separated in 2-DE according to their pI and molecular weight. (ashwinnaik.com)
  • This is the first report to systematically assess the antigenic characteristics of CAV viral proteins for sero-diagnosis purposes. (biomedcentral.com)
  • Alpha-crystallins also act as molecular chaperones that bind to denatured proteins, keep them in solution and thereby maintain the translucency of the lens. (lookformedical.com)
  • SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins. (ui.ac.id)
  • In addition, Gli-cyt was shown to possess charge and hydrophobic properties characteristic of natively unfolded proteins (i.e., proteins that, when purified, are intrinsically disordered under physiologic conditions in vitro). (ox.ac.uk)
  • MP is an acidic heteropolysaccharide with an average molecular weight (M w ) of 793.5 kDa. (nih.gov)
  • P contained 66.1% carbohydrate, 35.3% protein and 2.1% uronic acid and ranged in molecular weight from 850 to 20,000 Da. (scirp.org)
  • The molecular weight of this purified receptor could be determined by analysis on SDS-PAGE. (eurekamag.com)
  • SDS-gel electrophoresis results indicated that chicken erythrocyte catalase consists of four apparently identical subunits, with a molecular weight of around 57.5 kDa. (tubitak.gov.tr)
  • The average molecular weight (Mw) of purified EPS was 1.878 × 106 Da. (bvsalud.org)
  • At higher temperatures and longer reaction times, lignin with a high molecular weight was produced, which was attributed to the recondensation of lignin fragments. (ncsu.edu)
  • Thermal stability studies revealed that the increased molecular weight of DES-lignin enhanced its thermal resistance. (ncsu.edu)
  • The only significant changes occurred in molecular weight and gross surface morphology. (psu.edu)
  • A modest increase in weight average molecular weight was observed for most implanted blood sacs, indicating some type of chain extension or branching reaction in vivo. (psu.edu)
  • The catalytically active enzyme is a dimer of 113 KDa with a subunit molecular weight of 55 KDa. (scirp.org)
  • Serum albumin, which binds certain tiny molecular weight compounds in the blood, goes through a similar binding process. (modernbio.com)
  • By SDS polyacrylamide electrophoresis, up to 17 protein subunits in the molecular weight range of 14,000 to 87,500 were separated. (uni-muenchen.de)
  • The androgen testosterone (17 -hydroxyandrostenone) has a molecular weight of 288 daltons. (cdc.gov)
  • The second dimension is a normal SDS-PAGE, where the focused IPG strip is used as the sample, thus separating the samples bu their molecular weight. (ashwinnaik.com)
  • Database searches are then performed using both molecular weight and sequence information. (ashwinnaik.com)
  • It is adept at performing a wide range of applications such as formulation, deformulation of proprietary polymer blends, copolymer compositional drift or variation across the full Molecular Weight (MW) distribution, and process upsets. (spectra-analysis.com)
  • An aqueous extract of polysaccharides from Opuntia monacantha cladodes (POMC) was preliminarily purified by 5 kDa molecular weight cut-off ultrafiltration membrane to remove impurities with low molecular weight. (indexindex.com)
  • High-molecular-weight, low-substituted hydroxyethyl starch (HES) may not affect blood coagulation more than low-molecular-weight, low-substituted HES. (silverchair.com)
  • The authors assessed in vivo the effect of a lowered C2/C6 ratio on pharmacokinetic characteristics and the impact on blood coagulation of high-molecular-weight, low-substituted HES. (silverchair.com)
  • Although migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), under denaturing conditions, was unusually slow, molecular weight determination by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) confirmed that the product was consistent with its theoretical size. (ox.ac.uk)
  • However, in the presence of a fluctuating second or even third molecular characteristic, GPC/SEC cannot provide information regarding the polymer molar mass. (tsijournals.com)
  • The entropy controlled retention mechanism is paired with the interaction retention mechanisms to simultaneously identify two molecular features of a complex polymer such as copolymers or polymer blends Adsorption, or the distribution of a solute between a solid surface and a volume of its solution in a mobile phase, is the most prevalent interaction retention mechanism used in linked LC procedures. (tsijournals.com)
  • Gpc columns perform a separation based on the molecular size of polymer. (web.app)
  • Composed of semipermeable, porous polymer gel beads with well defined range of pore sizes. (web.app)
  • Molecular characterization of polysaccharides dissolved in. (web.app)
  • Systems that previously were considered too complex for a molecular characterization now successively become available to fundamental studies. (lu.se)
  • They were composed of glucose and mannose and 10 different amino acids and with molecular weights of 18,000 and 8000 Da, respectively. (scirp.org)
  • Shimpack gpc 80m mixed gel columns 80mc, 80md mixed gel columns that produce linear calibration curves over a wide range of molecular weights. (web.app)
  • In this approach the protein spot of interest is in-gel digested with a specific enzyme, the resulting peptides are extracted from the gel and the molecular weights of these peptides are measured. (ashwinnaik.com)
  • It is being undertaken using powerful analytical tools, like two-dimensional gel electrophoresis (2-DE) and ultrasensitive mass spectrometry (MS), coupled with high-throughput functional screening assays. (ashwinnaik.com)
  • Analytical TLC was carried out on Merck 60 F254 silica gel plate, and column chromatography was performed on Merck 60 silica gel (230 to 400 mesh) (Merck & Co., Inc., Whitehouse Station, NJ, USA). (springer.com)
  • Analytical and performance characteristics are not established. (bdbiosciences.com)
  • His research focused on thermodynamic linkage analysis of biological macromolecules, addressing the molecular mechanism of cooperative O2 binding to human hemoglobin since the early 1970s. (wikipedia.org)
  • An inert gas carries the sample through the column, and the partitioning of methoxychlor between the mobile and stationary phases gives it a characteristic retention time that is used to identify it. (cdc.gov)
  • Each PTH amino acid has a characteristic retention time in the RP-chromatography, enabling the identification of the residue by comparing to a standard. (ashwinnaik.com)
  • Bare silica gel is an acceptable stationary phase. (tsijournals.com)
  • The chemical attachment of suitable groups, usually C18 alkyl groups, onto a carrier, primarily silica gel, produces the practically applicable volume of LC stationary phase. (tsijournals.com)
  • In size exclusion chromatography, the stationary phase is. (web.app)
  • Standard Program 1 experiments are included in this lab activity, which emphasizes the importance of molecular biology in studying human genetics and disease. (modernbio.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • However with the rapid advances in biochemistry/molecular biology the colloid aspect of the fi eld was largely forgotten and colloid science was for a long period primarily a discipline for technical applications. (lu.se)
  • Basic concepts of colloid science like the surface force issue has wide applications in biochemistry and molecular biology. (lu.se)
  • Afin de remédier à ce problème et d'étudier les variations génétiques et antigéniques des virus A(H1N1)pdm09 et H3N2, nous avons procédé à des analyses génétiques et phylogénétiques des gènes de l'hémagglutinine (HA) et de la neuraminidase (NA) de ces virus, sur la période 2011-2013 en Jordanie. (who.int)
  • At 35 °C, two separate liquid chromatographic critical ranges were discovered using non-end-capped silica gel C-18 column packing, poly (methyl methacrylate)s (PMMAs), and mixed eluents including acetonitrile (ACN) and toluene. (tsijournals.com)
  • diffusion coefficient, molecular size, thermodynamics of protein-protein interactions including important changes due to single amino acid substitutions. (wikipedia.org)
  • Molecular sieve studies of interacting protein systems. (wikipedia.org)
  • Furthermore, gel column chromatography on Sephadex G-150 indicated that the elution curves of the carbohydrate and protein overlapped significantly in sample P ( Figure 1 ). (scirp.org)
  • The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method. (bvsalud.org)
  • In conclusion, the isolated cholesterol-containing urinary complex showed many characteristics of membrane-associated protein-lipid particles of the human kidney and even the liver. (uni-muenchen.de)
  • As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. (ui.ac.id)
  • Additionally, to bypass limitations of the gravimetric method a new measuring method based on gas-chromatography-mass-spectroscopy (GC-MS) technique was developed. (pe100plus.com)
  • Spectra Analysis Instruments, Inc. is a leading supplier of molecular spectroscopy systems and applications for chromatography. (spectra-analysis.com)
  • Jordanian H1N1 viruses had mutations that are characteristic of antigenic group 6 while H3N2 virus mutations belonged to group 3. (who.int)
  • Electron microscopy has showed a characteristic dimeric structure. (nature.com)
  • To screen and characterize the analgesic components from Marasmius androsaceus , the water-soluble extracts from the mycelium of M. androsaceus were isolated by ethanol precipitation followed by macroporous resin chromatography and activity-based fractionation. (scirp.org)
  • The hydrodynamic size of the molecules was followed using dynamic light scattering as a function of time and it was found that molecular association occurred in solution. (guildhe.ac.uk)
  • However, these molecules can easily form excimers through packing because anthracene and pyrene have flat molecular structure that reduce EL efficiency and degrade color purity [ 11 ]. (springer.com)
  • Pdf the mechanisms are discussed which control the gpc elution of ionic solutes, both the polyelectrolytes and the low molecular salts. (web.app)
  • molecular Mechanisms of lines. (scoutconnection.com)
  • Next, the physicochemical properties and characteristics of these purified glycopeptides were studied. (scirp.org)
  • Toward this end, as we reported herein, we separated and screened the water extract from the mycelium of M. androsaceus for the effective components, and subsequently, purified the active components to study their physicochemical properties and chemical characteristics. (scirp.org)
  • It has a primary amino acid sequence that is identical to the Ala148 allelic form of human factor IX, and has structural and functional characteristics similar to those of endogenous factor IX. (globalrph.com)
  • In the second approach peptides after in-gel digestion are fragmented in the mass spectrometer, yielding partial amino acid sequences from the peptides (sequence tags). (ashwinnaik.com)
  • Gary Keith Ackers (1939 - 2011) was Emeritus Professor of Biochemistry and Molecular Biophysics of Washington University in St. Louis, Missouri. (wikipedia.org)
  • Theory of separation a column is made up of swollen gel particles and the solvent used to swell the gel in a suitable tubular container. (web.app)
  • The chemical and physicochemical characteristics of gum exudate samples harvested from mature trees of Acacia senegal at two new specific ecolocations in Nigeria, have been investigated together with gum samples harvested from A. senegal and A. seyal originating from Sudan. (guildhe.ac.uk)
  • Besides various excellent physical properties, including high elasticity and high tensile strength, NR also has some undesirable properties such as low heat resistance and poor aging properties, as well as low oil resistance due to the low saturation degree of the molecular chain [ 1 , 6 - 8 ]. (hindawi.com)
  • Reducing the C2/C6 ratio in high-molecular, low-substituted HES solutions results in a slightly faster HES elimination. (silverchair.com)
  • Nasopharyngeal (NP) and oropharyngeal (OP) swab specimens were collected on day 3 postsymptom onset, placed in 2-3 mL of viral transport medium, used for molecular diagnosis, and frozen. (cdc.gov)
  • The formation of a network structure was characterized by sol/gel analysis. (pe100plus.com)
  • The molecular structure of probes is easily visualized under AFM. (molvis.org)
  • AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes. (molvis.org)
  • The optical spectrum of the purified enzyme shows a Soret band at 406 nm, which is the characteristic for the heme group. (tubitak.gov.tr)
  • Some specific molecular interactions that occur in biological systems include the binding of an enzyme to its substrate. (modernbio.com)
  • Deep eutectic-like solvent-assisted isolation of lignin from Pinus densiflora and its characteristics ," BioResources 17(4), 5600-5611. (ncsu.edu)
  • In this article, we describe isolation of SARS-CoV-2 from a patient who had coronavirus disease (COVID-19) in the United States and described its genomic sequence and replication characteristics. (cdc.gov)
  • Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo. (nih.gov)
  • Our laboratory has the Testing Laboratory Accreditation Certificate in accordance with the requirements of the PN-EN ISO / IEC 17025: 2018-02 standard. (jakusz-spacetech.com)
  • The plot of radius of gyration, Rg, as a function of elution volume showed a discontinuity for one of the Nigerian samples and for the A. seyal gum sample at elution volumes corresponding to the AGP component suggesting a different molecular structure. (guildhe.ac.uk)
  • A material profile is made up of numerous requirements - be it mechanical, thermal or rheological or completely different characteristics that make up the essence of a plastic. (skz.de)