Molecular cell proteins clone cells. Medical search. Frequent questions

This platform is built on the company's Lipoparticle and Shotgun Mutagenesis technologies and over 15 years of experience optimizing membrane proteins, enabling the isolation, characterization, and engineering of monoclonal antibodies against otherwise intractable membrane protein targets. (integralmolecular.com)
As a senior scientist in the Hi-TIDe , I'm currently responsible for the high-throughput discovery and characterization of cellular immune responses directed against unknown, shared and private tumor epitopes and participate in the development of new adoptive cell therapy strategies. (unil.ch)
Publications] Kaibuchi,K.: 'Molecular cloning and characterization of the stimulatory GDP/GTP exchange protein(GDS)for smg p21s,ras p21ーlike small GTPーbinding proteins. (nii.ac.jp)
Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins. (oregonstate.edu)
We offer products for molecular purification and characterization, media prep, analytical chemistry and microbiology. (drugdiscoveryonline.com)

Conversion of this insoluble material involves often slow and complex refolding methods, making protein purification difficult. (justia.com)
Doctoral students at NU have access to many state-of-the-art laboratories for conducting advanced research in biochemical, microbiological and molecular techniques such as electrophoresis studies, immuno-technology studies, protein expression and purification, DNA/RNA isolation, PCR, genomics applications, cell culture technology, molecular cloning methodologies and bioinformatics software applications while adhering to appropriate biosafety measures. (niituniversity.in)

Production of the fusion polypeptide involves the introduction of the hybrid gene into a biological host cell system, for example yeast cells, which permits the expression and accumulation of the fusion polypeptide. (justia.com)
Morscheid S., Rey-Rico A., Schmitt G., Madry H., Cucchiarini M., Venkatesan J.K. Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-β and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells. (uniklinikum-saarland.de)
The molecular mechanisms potentially responsible for cadmium carcinogenesis were investigated by differential gene expression analysis of Balb/c-3T3 cells morphologically transformed with cadmium chloride. (cdc.gov)
Combining these new datasets with existing published gene expression and protein expression data have identified selected panels of novel biomarkers which have been evaluated on over 100 cervical cancer and pre-cancer cases using standard immunohistochemistry approaches. (europa.eu)
The sequence of this gene is defined by 16 cDNA clones and 18 elements defined by RNA-seq, some from mixed (seen 7 times), embryo (5), l2 (once). (nih.gov)
This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones. (nih.gov)
We and other groups discovered the human IL-17F gene from a human EST sequence, a genomic DNA clone, and T-cell cDNA sequences in 2001 [ 3 , 8 , 9 ]. (hindawi.com)
Specificity Comments: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. (thermofisher.com)
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. (thermofisher.com)
Identified gene candidates will be studied further in the context of determining sensitivity of protein degrader drugs which are currently emerging as alternative therapies for leukemia. (lu.se)
At some centers, tests are done to look for gene or chromosome abnormalities usually present in myelodysplastic syndrome (sometimes called molecular testing). (msdmanuals.com)

The lab uses a wide range of approaches in our projects: molecular biology, protein biochemistry, in vitro cell culture models, xenograft and syngeneic mouse models, genetically engineered mouse models, various CRISPR-based techniques, and genomic/transcriptomic/proteomic omics techniques. (postdocjobs.com)
I have a PhD in Biochemistry and Molecular Biology. (unil.ch)
After obtaining my Master's in Biochemistry at the University of Geneva in 1994, I worked for Glaxo and Serono Pharmaceutical Research Institute in the Chemokines and Functional Genomics fields where I acquired a variety of techniques in molecular and cellular biology. (unil.ch)

Fluorescent signals are emitted when antibody recognition occurs within the droplet, allowing laser-sorting of droplets and B-cell isolation. (integralmolecular.com)
This approach enables rapid interrogation of a host animal's entire immune repertoire, antibody isolation directly from host cells for natural heavy and light-chain pairing, and isolation of diverse antibodies. (integralmolecular.com)
The combination of antigen display on Lipoparticles, use of evolutionarily divergent species for antibody elicitation against conserved targets, and now B-cell cloning for rapid isolation of rare antibodies enable us to deliver first-in-class therapeutic candidates to our discovery partners. (integralmolecular.com)
Design, engineering and application of pMHC class I and II molecules for the detection and isolation of antigen-specific T cells. (unil.ch)
This methodology facilitates the isolation and recovery of the fusion peptide from the host cells by exploiting the physico-chemical properties of the fusion partner. (justia.com)

Extensive evidence has revealed that the core clock machinery involves "clock genes" and "clock proteins" functioning as molecular cogs. (bioone.org)
Transcriptional profiling of these candidate GST genes and key anthocyanin biosynthetic pathway genes (PAL, CHS, DFR, and UFGT) in cell suspensions and grape berries against anthocyanin accumulation demonstrated strong positive correlation with two sequences, VvGST1 and VvGST4, respectively. (oregonstate.edu)
The thesis work would focus on identification of genes that are essential for the optimal functioning of the ubiquitin-proteasomal system in leukemic cells. (lu.se)

B-cell cloning represents a pivotal expansion of the company's MPS Discovery Engine® platform for isolating monoclonal antibodies against membrane protein targets. (integralmolecular.com)
Combined with the company's proprietary Lipoparticle technology for display of concentrated membrane proteins, antibodies are isolated that recognize membrane proteins in their native conformation, these being the most useful class of antibodies for therapeutics. (integralmolecular.com)
This technology transforms antibody discovery for membrane protein targets," says Dr. Ross Chambers, Director of Antibody Discovery and developer of the B-cell cloning technology. (integralmolecular.com)
Most plasma membrane proteins are sorted by the trans -Golgi network (TGN) in the conventional secretory pathway. (jneurosci.org)
A cDNA clone coding for a membrane proteoglycan core protein was isolated from a neonatal rat Schwann cell cDNA library by screening with an oligonucleotide based on a conserved sequence in cDNAs coding for previously described proteoglycan core proteins. (rupress.org)
The prokaryotes are a group of organisms that lack a cell nucleus , or any other membrane-bound organelles. (absoluteastronomy.com)
What is now clear is that certain membrane lipids can function as messenger signals that regulate cell growth, proliferation, inflammation, and programmed cell death processes, while other membrane lipids appear to cluster together in bilayers to form microdomains that regulate the spatial distribution and lateral interactions of membrane proteins. (umn.edu)
The discovery of these membrane lipid functions underscores why cell membranes so often come under direct attack during cancer and infectious disease. (umn.edu)
Our research focuses on membrane lipids known as sphingolipids and on proteins that interact in non-enzymatic fashion with sphingolipids. (umn.edu)
We have since focused our efforts on specific proteins that can bind and transfer sphingolipids between membrane surfaces and that may help formation and maintain 'raft' microdomains in cells. (umn.edu)
Outer mitochondrial membrane permeabilization and cytochrome c release are typically achieved by pro-apoptotic Bcl-2 proteins, such as Bax and Bak. (nature.com)
It is a single-chain membrane glycoprotein of approximately 200 kd that has 4 allotypic forms on myeloid cells (eg, erythrocytes, granulocytes, monocytes), lymphoid cells (mostly B cells), follicular dendritic cells, and glomerular podocytes. (medscape.com)

Among these regulatory proteins, we have cloned the cDNAs of smg p21 GDS, smg p25A GDI, and rho GDI, and made monoclonal antibodies against them. (nii.ac.jp)
The full length cDNAs for TIF3 and TEF-1d were cloned and sequenced. (cdc.gov)

Primer extension and polymerase chain reaction amplification were used to obtain additional 5' protein coding sequences. (rupress.org)
In molecular biology and genetics, a transcription factor is a protein that binds to specific DNA sequences, thereby controlling the flow of genetic information from DNA to mRNA. (absoluteastronomy.com)
The corresponding coding sequences were cloned, with heterologous expression in Escherichia coli used to confirm GST activity. (oregonstate.edu)
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. (nih.gov)

Here, we report the establishment and the characteristics of persistent in vitro infection of human hepatoma-derived cells by a recently described HCV genotype 2a infectious molecular clone. (nih.gov)
Further investigation of these candidate ligandins may identify a mechanism for manipulating anthocyanin accumulation in planta and in vitro suspension cells. (oregonstate.edu)

Integral Molecular, a leader in antibody discovery, announces the development of an ultra high-throughput microfluidic B-cell cloning technology that can interrogate tens of millions of immune cells (B-cells), enabling deep mining of entire immune repertoires to discover rare and diverse monoclonal antibodies for therapeutic purposes. (integralmolecular.com)
In this research project, we have investigated the C-terminal structures of small G proteins, purified the regulatory proteins for small G proteins, and made monoclonal antibodies against small G proteins and their regulatory proteins. (nii.ac.jp)

Based on enzymatic studies, that is galactosylation and its inhibition, conformational analysis and molecular modeling using the crystal structure, we propose that the binding pocket of beta 4GalT7 is very narrow, with a precise set of important hydrogen bonds. (lu.se)

However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
In parallel, as the virus became more aggressive, cells that were resistant to infection emerged, displaying escape mechanisms operative at the level of viral entry, HCV RNA replication, or both. (nih.gov)
Tricyclics are thought to exert their therapeutic effect through chronic blockade of presynaptic serotonin and/or norepinephrine transporters ( Frazer, 1997 ), but the molecular mechanisms mediating many of the deleterious side effects have not been identified. (jneurosci.org)
Hence, increased understanding of the significance of IL-17F would help to uncover the molecular mechanisms of asthma. (hindawi.com)

J Cell Biol (1992) 117 (1): 191-201. (rupress.org)
Nat Rev Mol Cell Biol , Art. (mpi-cbg.de)
Mol.Cell.Biol. (nii.ac.jp)

MD or PhD in Cancer Biology, Molecular and Cellular Biology, Genetics or related. (postdocjobs.com)

Three murine members of the RXR family (mRXR alpha, beta, and gamma) have also been cloned, and their interactions with RARs and TRs have been investigated. (nih.gov)
Nanoscale imaging enables the analysis of biological systems, such as cells and tissues, at the fundamental length scales of biomolecules and biomolecular interactions, but classically has required expensive equipment and advanced skillsets to perform, e . g ., via super-resolution microscopy [ 1 ]. (plos.org)
Moreover, oxidation of GRX-I introduced an extra α-helix increasing the destabilizing interactions within the protein. (bvsalud.org)
Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)

The technology entails encapsulating individual antibody secreting B-cells, together with antigen displaying Lipoparticles, into picoliter-sized droplets. (integralmolecular.com)
Structure, function, and serology of the T-cell antigen receptor complex. (bdbiosciences.com)
Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. (bdbiosciences.com)
The antigen corresponds to amino acid range 1-50 of the target protein. (thermofisher.com)
This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). (sigmaaldrich.com)
Qualitative or quantitative defect in protein S. ELISA for free protein S antigen. (medscape.com)

Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
Partial or complete deficiencies of the components of the complement system, including its receptors and regulatory proteins, are now described in humans and may be of a genetic or familial origin or acquired. (medscape.com)

On Northern blots the cDNA hybridized to a single 5.6-kb mRNA that was present in Schwann cells, neonatal rat brain, rat heart, and rat smooth muscle cells. (rupress.org)
A ribonuclease protection assay was used to quantitate levels of the core protein mRNA. (rupress.org)
We have found that the levels of smg p21 mRNA increase in hematopoietic leukemiae cells after their differentiation into hematopoietic cells. (nii.ac.jp)
We have also found that smg p25A is mainly detected inneural tissues by use of its specific monoclonal antibody, and that the levels of smg p25A mRNA increase in PC12 cells after their differentiation into neuron-like cells. (nii.ac.jp)
Blocking the translation of TIF3 and TEF-1d proteins using the corresponding antisense mRNA resulted in a significant reversal of the oncogenic potential of cadmium transformed Balb/c-3T3 cells as evidenced from the suppression of anchorage-independent growth on soft agar and diminished tumorigenesis in nude mouse. (cdc.gov)
Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)

The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. (justia.com)
The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions. (justia.com)
The preparation of valuable recombinant (genetically engineered) polypeptides, for example pharmaceutical proteins, relies frequently on techniques which involve the production of these polypeptides as fusion or hybrid proteins. (justia.com)
Finally, the use of a fusion partner may enable the production of a peptide which would otherwise be too small to accumulate and recover efficiently from a recombinant host cell system. (justia.com)
The following product was used in this experiment: SOX2 (Embryonic Stem Cell Marker) Recombinant Rabbit Monoclonal Antibody (SOX2/4267R) from Thermo Fisher Scientific, catalog # 6657-RBM7-P1. (thermofisher.com)
Recombinant fragment of human SOX2 protein. (thermofisher.com)

Transfection of mammalian cells with an expression vector containing either TIF3 or TEF-1d cDNA resulted in overexpression of the encoded protein. (cdc.gov)
Thus, we conclude that ADF/cofilin activity is not generally required for induction or progression of apoptosis in mammalian cells. (nature.com)
In summary, our work demonstrates that in mammalian cells ADF/cofilin activity is not generally required for efficient induction of apoptosis. (nature.com)
Mammalian cells produce PGs, which are involved in biological processes such as cell growth and differentiation. (lu.se)

An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. (thermofisher.com)

Freidrich W, O'Reilly R, Kozenger B, Gebhard D, Good R, Evans R. T lymphocyte reconstitution in recipients of bone marrow transplants: Analysis of regulatory T-cell imbalances in GVHD. (bdbiosciences.com)
These findings demonstrate an intricate link between GLRX and GSH via S-glutathionylation of OTUB1 and system xC- and illuminate a previously unknown feed-forward regulatory mechanism whereby enhanced GSH protein oxidation augments cellular GSH. (bvsalud.org)

A germ cell is any biological cell that gives rise to the gametes of an organism that reproduces sexually. (absoluteastronomy.com)
The students know the structure and function of proteins and enzymes, as well as the role of metal ions in biological systems. (uni-leipzig.de)
The topics of the bachelor's theses are methodologically predominantly molecular biological and biochemical in nature. (uni-leipzig.de)

Integral Molecular discovers antibodies for partners in parallel with its own independent work developing antibodies for licensing. (integralmolecular.com)
The resulting antibodies reacted on immunoblots with the core protein of a detergent extracted heparan sulfate proteoglycan. (rupress.org)
When the anti-core protein antibodies were used to stain tissue sections immunoreactivity was present in peripheral nerve, newborn rat brain, heart, aorta, and other neonatal tissues. (rupress.org)

We are interested in understanding how these modifications are misregulated in tumor cells, tumor-infiltrating immune cells, and tumor-interacting stroma cells. (postdocjobs.com)
Our current projects include the multifaceted roles of ITCH in melanoma and the melanoma immune microenvironment, the context-dependent functions of master cell cycle regulator APC/C in breast cancer and ovarian cancer, the crosstalk between tumor cell aneuploidy and innate immunity, and several others. (postdocjobs.com)
Both the structure of the PGs and the GAG chains, and the expression of the enzymes involved in their biosynthesis and degradation, vary between normal cells and tumor cells. (lu.se)

The findings have proven to be important for current understanding how the spatial organization of lipids in membranes can regulate proteins that translocate onto membranes to function. (umn.edu)

The CD3 antibody, clone SK7, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with human thymocytes. (bdbiosciences.com)
This Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60. (sigmaaldrich.com)

The 3 spliced mRNAs putatively encode good proteins , altogether 3 different isoforms (1 complete, 2 partial ), some containing a coiled coil stretch [Psort2] . (nih.gov)

Endogenous substances are those that originate from within an organism, tissue, or cell. (absoluteastronomy.com)
A provirus is a virus genome that is integrated into the DNA of a host cell.This state can be a stage of virus replication, or a state that persists over longer periods of time as either inactive viral infections or an endogenous retrovirus. (absoluteastronomy.com)

As a technician within the Hi-TIDe , I closely interact with different scientists, analyse and discuss data aiming at the development of new assays with a special focus on TCR cloning. (unil.ch)
Since joining the Department of oncology UNIL CHUV in 2013, I performed, validated and implemented cellular and molecular assays to interrogate T cells from clinical samples for research and in the context of clinical trials (immunological monitoring). (unil.ch)

Cell Reports 2022. (postdocjobs.com)

The four conserved cysteines in the C-terminal half of the IL-17F sequence are shown to form a cystine knot structural motif in the crystal structure, and, interestingly, this cystine knot structure is similar to a common structural motif seen in several growth factors, such as bone morphogenic proteins (BMPs), TGF- β , nerve growth factor (NGF), and platelet-derived growth factor (PDGF) [ 9 ]. (hindawi.com)
The alpha granules contain hemostatic proteins such as fibrinogen, vWf, and growth factors (eg, platelet-derived growth factor and transforming growth factors). (medscape.com)

In ciliated cells, including bovine and Xenopus laevis rod photoreceptors, P/rds was robustly sensitive to endoglycosidase H, which is consistent with its bypassing the medial Golgi and traversing the unconventional secretory pathway. (jneurosci.org)

Such proteins include sphingolipid transfer proteins that function as transporters to shuttle sphingolipids between intracellular membranes as well as lipid-binding domains (e.g. (umn.edu)

The molecular building blocks of bilayer membranes are specialized lipids that are polar and nonpolar at their opposite ends. (umn.edu)

They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. (sigmaaldrich.com)

Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. (thermofisher.com)

Evidence is accumulating that small G proteins are involved in the regulation of cell growth and differentiation. (nii.ac.jp)

I am involved in developing a high-throughput platform to interrogate patients' immunity against private antigens and to identify highly functional T cells (and TCRs) for adoptive cell therapy (ACT). (unil.ch)

Since joining the Hi-TIDe in 2013, I develop new molecular tools to analyze and characterize TCR. (unil.ch)
Our products are designed to quantify and characterize host cell proteins in process-derived samples harvested from CHO supernatant. (drugdiscoveryonline.com)

The sensory primary cilium of vertebrate photoreceptor cells houses thousands of photosensitive disk membranes that are renewed continuously throughout our lifespan. (jneurosci.org)
We have found that the C-terminal systeine residues of smg p21B, rhoA p21, and smg p25A are geranylgeranylated, and that these prenylation are essential for each small G protein to bind to membranes. (nii.ac.jp)
Publications] Araki,S.: 'Role of the Cーterminal region of smg p25A in its interaction with membranes and the GDP/GTP exchange protein. (nii.ac.jp)
Eukaryotic cells, including human cells, surround themselves with bilayer membranes, i.e. thin, flexible layers that are only two molecules thick. (umn.edu)
Bilayer membranes also serve as internal partitions to enable formation of functionally-specialized compartments within cells. (umn.edu)
These sphingolipid transfer proteins were initially identified as glycolipid transfer proteins (GLTPs) due to their specificity for transferring glycosphingolipids between membranes. (umn.edu)
They are single-chain molecules present on host cellular membranes and belong to the complement control protein family. (medscape.com)
The sphingomyelin that accumulates in the lysosomes of Niemann-Pick disease-affected cells is thought to arise from the degradation of the cells and their organelles because it is a major component of all mammalian cell membranes. (medscape.com)

It has been recently shown that mutations of lens gap junction proteins are associated with certain forms of inherited congenital cataract. (rosalindfranklin.edu)

We have purified and cloned a HeLa cell nuclear protein that strongly stimulates binding of retinoic acid and thyroid hormone receptors (RARs and TRs) to response elements. (nih.gov)
Effector proteins interacting with the Fc portion of immunoglobulin M (IgM) include complement and complement receptors. (medscape.com)
Defects in the expression of complement or complement receptors may result in loss of tolerance to self-proteins and the development of immune complex-mediated autoimmune diseases such as systemic lupus erythematosus (SLE). (medscape.com)
The density of CR1 receptors on cell surfaces varies with the cell type and with the activation of the cell for neutrophils and monocytes. (medscape.com)
Because of their high numbers, red blood cells express an average of 90% of the CR1 receptors despite their having a lower number of antigenic sites per cell. (medscape.com)
Among healthy individuals, the number of CR1 receptors on the red blood cells varies widely, yet the phenotypic expression, regulated by 2 codominant alleles, is stable. (medscape.com)

The purified protein is a human retinoid X receptor beta (hRXR beta). (nih.gov)
In 2008, I joined the the Department of oncology UNIL CHUV in the laboratory of Pr Immanuel Luescher where I started to study CD8 T-cells and their receptor (TCR). (unil.ch)
Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. (bdbiosciences.com)
Mice with C5a receptor-deficient dendritic cells promote induction of Treg and Th17 cells. (medscape.com)
CR1 and CR2 have been shown to influence the immune environment in a B-cell receptor-independent manner. (medscape.com)

s refers to those that are manufactured by the cell, as opposed to cloned transcription factors. (absoluteastronomy.com)

Tong JJ, Acharya P, Ebihara L. Calcium-Activated Chloride Channels in Newly Differentiating Mouse Lens Fiber Cells and Their Role in Volume Regulation. (rosalindfranklin.edu)
The reversible reduction and oxidation of protein thiols was first described as mechanism to control light/dark-dependent metabolic regulation in photosynthetic organisms. (bvsalud.org)
Glutaredoxin (GRXs) protein plays a vital role inside the cell, including redox control of transcription to the cell's antioxidant defense, apoptosis, and cellular differentiation regulation. (bvsalud.org)

These proteins underwent Edman sequencing and mass spectrometry fingerprinting, with the resultant fragments aligned with predicted GSTs within public databases. (oregonstate.edu)

International Journal Of Molecular Sciences , 2019, 27;20(10). (uniklinikum-saarland.de)

Frontiers in Molecular Neuroscience. (lu.se)

This review summarizes evidence for the role of proteolytic enzymes that degrade and inactivate insulin-like growth factor binding proteins (IGFBP) during follicular development in mammals. (bioone.org)
This protease activity has recently been ascribed to serine metalloprotease(s), including pregnancy-associated plasma protein-A (PAPP-A), which was first detected in human follicular fluid nearly 20 yr ago. (bioone.org)

Interestingly, mitochondrial association of ADF and n-cofilin during apoptosis was preceded by, and dependent on, actin that translocated by a yet unknown mechanism to mitochondria during cell death. (nature.com)
The mosquito protein AEG12 displays both cytolytic and antiviral properties via a common lipid transfer mechanism. (ncsu.edu)

All of these procedures result in the production a hybrid protein in which the protein of interest is linked to an additional polypeptide. (justia.com)
During further processing, the 110-kd polypeptide is reduced to a molecular weight of 84 kd. (medscape.com)

Leucocyte Typing IV: White Cell Differentiation Antigens. (bdbiosciences.com)
Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. (bdbiosciences.com)

These proteins have a rather broad but at the same time distinct substrate specificity. (bvsalud.org)
Understanding the molecular basis of their target specificity is central to the understanding of physiological and pathological redox signaling. (bvsalud.org)

The organisms attach to the epithelial cells of the upper respiratory tract and produce a battery of enzymes allowing them to damage and invade human tissues. (medscape.com)

as it relates to the cellular and molecular responses to damage [email protected]. (cdc.gov)

Topic of the lecture are biochemical basics and methods in the production and analysis of proteins and DNA. (uni-leipzig.de)

The monolayer format produces a continuous layer of stem or differentiated cells on a flat and porous surface. (drugdiscoveryonline.com)

IL-17F is expressed in activated CD4 + T cells, basophils, and mast cells, three important cell types involved in allergic airway inflammation [ 3 ]. (hindawi.com)
Treatment Acute myeloid leukemia is a life-threatening disease in which the cells that normally develop into the types of white blood cells called neutrophils, basophils, eosinophils, and monocytes become. (msdmanuals.com)

We have purified and characterized several GDP/GTP exchange proteins (GDP dissociation stimulator (GDS) and GDP dissociation inhibitor (GDI)) and GTPase activating Proteins (GAP) for small G proteins. (nii.ac.jp)

The specimens were then processed and transported to the reference laboratory of Institute of Epidemiology, Disease Control and Research (IEDCR) and icddr,b for confirmation of diagnosis through serology and molecular detection. (cdc.gov)

uniExM may find many uses in the simple, multimodal nanoscale analysis of cells and tissues. (plos.org)

We hypothesize that EGL-2( gf ) channels cause behavioral defects through suppression of excitability in critical cells. (jneurosci.org)
green fluorescent protein (GFP) fusions revealed egl-2 expression in a subset of neurons and muscles that could explain the mutant behavioral defects. (jneurosci.org)
Consequently, several human diseases, including cancer and neurodegenerative disorders, are caused by defects in this highly regulated form of cell death. (nature.com)
We annotate structural defects or features in 6 cDNA clones. (nih.gov)
Protein defects. (medscape.com)

The work is either for the preparation of protein for crystallization and structural analysis or for functional studies by mutagenesis. (uni-leipzig.de)
To determine the structural requirements for beta 4GalT7 activity, we have cloned and expressed the enzyme and designed a focused library of 2-naphthyl beta-D-xylopyranosides with modifications of the xylose moiety. (lu.se)

A postdoctoral position is available in Dr. Lixin Wan's group at Moffitt Cancer Center Department of Molecular Oncology. (postdocjobs.com)
After obtaining my Master's in Medical Biotechnology in Padua and Molecular Biology, in Bologna, I undertook a PhD in Oncology at the University of Padua, which is where my first interest in T cell engineering, and clinically oriented immunotherapy protocols developed. (unil.ch)

Anatomy35

Organisms27

Diseases3

Chemicals and Drugs92

Analytical, Diagnostic and Therapeutic Techniques and Equipment40

Phenomena and Processes59

Disciplines and Occupations4

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care1

Characterization5

Purification2

Gene11

Biochemistry3

Isolation5

Genes3

Membrane12

CDNAs2

Sequences4

Vitro2

Monoclonal antibodies2

Conformational1

Mechanisms5

Biol3

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MRNA6

Recombinant6

Mammalian cells4

Embryonic stem1

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Translocate1

Antibody2

Different isoforms1

Endogenous2

Assays2

20221

Platelet-derive2

Xenopus1

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Lipids1

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Transcriptional1

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Interrogate1

Characterize2

Membranes8

Mutations1

Receptors6

Receptor5

Transcription Factors1

Regulation3

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20191

Neuroscience1

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Epithelial cells1

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Defects5

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