Proteins2022High-throughputGenomics and proteomics2021BiologyFunctionalProteinApoptosisPathwaysMechanismsJournalApproachesOxidativePhysiologyLund UniversityAnalysisAffinityAssaysInteractionsGeneticsOncologyQuantitativeTissuesCellsProteasesTransitionCentreStudyDataVitroDefineInvestigationsMedicineUniversityFunction
Proteins11
- In addition, the molecular function of some of the described proteins suggests a central role for SVEVs in the cytotoxicity of the snake venom and sheds new light in the envenomation process. (nature.com)
- By examining changes in various proteins involved in the wound healing response at the cellular level, Segars hopes to understand why the corneas of diabetic patients fail to heal effectively. (bu.edu)
- While proteomics typically aims to quantify as many proteins as possible, many of the biological questions that are to be addressed also necessitate the processing of large sample series. (elifesciences.org)
- I joined the department of Molecular Biology as Scientist in 1989, and my lab helped establish a technology to fuse biologically important proteins to antibody molecules. (gene.com)
- Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
- Mariana Torrente received dual bachelor degrees in Chemistry and Biochemistry from the Florida Institute of Technology in 2005 and her Ph.D. in Chemistry in 2010 from Princeton University where she comprehensively cataloged chromatin proteins -particularly histones- during biologically significant processes using high-end mass spectrometry proteomics. (cuny.edu)
- The study shows the usefulness of proteomics in identifying proteins that may express differences between sensitive and resistant L. tropica isolates. (who.int)
- Recent evidence indicates that, apart from conserved molecular allostery, Hsp70 proteins have retained and adapted the ability to assemble as functionally relevant ATP-bound dimers throughout evolution. (muni.cz)
- Cancer stem cell-associated proteins revealed by proteomics. (medscape.com)
- The RPS19 gene provides instructions for making one of approximately 80 different ribosomal proteins, which are components of cellular structures called ribosomes. (medlineplus.gov)
- The main aim of our research is to understand the molecular details and physiological roles of the multitude of water channel-like proteins present in plants and animals. (lu.se)
20221
- Molecular Biology and Evolution , 2022, Roč. (cuni.cz)
High-throughput3
- We have a major focus on bowel cancer genomics, the application of high-throughput molecular profiling technologies to identify principal cancer genes and biomarkers of cancer risk and prognosis. (edu.au)
- Zeno SWATH MS hence facilitates sensitive high-throughput proteomic experiments with low sample amounts, mitigating the current bottlenecks of high-throughput proteomics. (elifesciences.org)
- In proteomics, high throughput approaches using mass spectrometry have be- come widely used. (lu.se)
Genomics and proteomics2
- The idiosyncratic nature of virus discovery contrasts with the broad survey approaches characteristic of genomics and proteomics. (cdc.gov)
- PhD in Genomics and Proteomics, Masaryk University, Czech Republic. (lu.se)
20215
- 2021. "Fate, cytotoxicity and cellular metabolomic impact of ingested nanoscale carbon dots using simulated digestion and a triculture small intestinal epithelial model. (pnnl.gov)
- 2021. "Enhancing top-down proteomics of brain tissue with FAIMS. (pnnl.gov)
- 2021. "Assessment of TMT labeling efficiency in large-scale quantitative proteomics: the critical effect of sample pH. (pnnl.gov)
- 2021. "Characterization of cellular oxidative stress response by stoichiometric redox proteomics. (pnnl.gov)
- 2021. "Stoichiometric Thiol Redox Proteomics for Quantifying Cellular Responses to Perturbations. (pnnl.gov)
Biology8
- Molecular & Cellular Proteomics is a monthly peer-reviewed scientific journal established in 2002 and published by the American Society for Biochemistry and Molecular Biology. (wikipedia.org)
- Cell, Developmental, and Molecular Biology. (rochester.edu)
- Molecular & Cellular Biology. (rochester.edu)
- Responses to abiotic and biotic stresses - from the cellular level to fruit development - contributions of the Czech Centre for Experimental Plant Biology. (muni.cz)
- Journal of Molecular Biology, 256:201-213, 1996. (uni-marburg.de)
- Here, the authors review the novel insights that these studies have provided and present a comprehensive strategy for the use of proteomics in studying cancer SC biology. (medscape.com)
- PhD in Molecular and cellular biology, University of Poitiers, France. (lu.se)
- Molecular Membrane Biology 2013, 30 (3):246-260. (lu.se)
Functional4
- Patient-focused investigations are being complemented with functional studies on cancer cell lines to distinguish driver from passenger mutations and to gain insights into molecular pathways of carcinogenesis. (edu.au)
- HADHB is a functional molecular target of ERα in the mitochondria, and the interaction may play an important role in the estrogen -mediated lipid metabolism in animals and humans. (wikidoc.org)
- Moreover, dimerization of ATP-bound Hsp70 is required for its participation in high molecular weight protein complexes detected ex vivo, supporting its functional role in vivo. (muni.cz)
- To advance the field, we collaborate closely with clinicians to perform advanced molecular investigations of patient material derived from within and outside clinical trials, including sequencing, spatial omics technologies, serum protein profiling and functional screening. (lu.se)
Protein11
- A main bottleneck in proteomics is the downstream biological analysis of highly multivariate quantitative protein abundance data generated using mass-spectrometry-based analysis. (nih.gov)
- In a recent paper in the journal Molecular & Cellular Proteomics , Tirsa L.E. van Westering and researchers at the University of Oxford used mass spectrometry to identify differential protein expression in muscles at three different ages (representing the different stages of DMD pathology) from two strains of mice with genetically altered DMD and one strain of unaltered mice (control). (asbmb.org)
- Enzymatic digestion of protein into shorter peptides by sequence-specific proteases is a key step in mass spectrometry-based shotgun proteomics experiments. (asbmb.org)
- We further wanted to ascertain whether this protein interaction was direct using recombinant versions of purified b-catenin and WT1 protein but failed to detect any association ( Online Supplementary Figure S1B, C ). This suggested that perhaps the interaction is mediated through a post-translational modification, common partners such as WTX, 8 or cellular structures like DNA, given their well-documented roles in transcription. (haematologica.org)
- Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)
- ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)
- Eukaryotic protein homeostasis (proteostasis) is largely dependent on the action of highly conserved Hsp70 molecular chaperones. (muni.cz)
- The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
- The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
- A particular focus is development of new proteomics techniques to investigate protein interactions between host and pathogen and to investigate systemic proteome changes during sepsis. (lu.se)
- Two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) based proteomics was used to study differential protein abundance in various B cell types during B cell maturation (cell lines are listed in Supplementary Table 1 ). (lu.se)
Apoptosis1
- Does oxidative stress represent a secondary event resulting inevitably from disruption of biochemical processes and the demise of the cell, or a specific, non-random event that plays a role in the induction of cellular damage e.g. apoptosis? (cdc.gov)
Pathways2
- Proteomics is emerging as a powerful tool to identify the signaling complexes and pathways that control multi- and pluri-potency and SC differentiation. (medscape.com)
- DAMPs, damage-associated tissues and the molecular target structures and pathways. (cdc.gov)
Mechanisms4
- His research focus is to develop and apply proteomics based techniques to characterize the molecular mechanisms behind invasive bacterial infection. (lu.se)
- During sepsis, these host defence systems become pathologically augmented and deregulated and we develop experimental models to delineate cellular and molecular mechanisms involved. (lu.se)
- His research aims to elucidate molecular mechanisms underlying bacterial interactions with human cells and to use that information to identify targets for treatment and diagnostics of bacterial infections. (lu.se)
- Figuring out molecular mechanisms of cancer and normal development. (lu.se)
Journal5
- Journal of Molecular Diagnostics. (edu.au)
- International Journal of Molecular Sciences, 24 (1). (sussex.ac.uk)
- International Journal of Molecular Sciences, 22 (21). (sussex.ac.uk)
- The researchers also demonstrated ProAlanase's broad utility in other proteomics applications in a recent paper published in the journal Molecular & Cellular Proteomics . (asbmb.org)
- In a recent paper in the journal Molecular & Cellular Proteomics , Greta Forlani and a team of Swiss researchers write that they discovered how to induce HLA-II expression by inserting the gene histocompatibility complex transactivator, or CIITA, into various GMB cell lines. (asbmb.org)
Approaches2
- Recent examples of global lipidomics studies of nanoparticle -induced tissue damage are discussed along with proteomics and transcriptomics approaches to achieve a comprehensive understanding of the complex and interrelated molecular changes in cells and tissues exposed to nanoparticle s. (cdc.gov)
- [ 5 ] Obviously, the study of CSCs in any given tumor type, by proteomics or other approaches, requires prior validation of their actual existence. (medscape.com)
Oxidative1
- We also discuss instances of non-oxidative stress-mediated cellular damage resulting from direct physical interference of nanomaterial s with cellular structures. (cdc.gov)
Physiology1
- PI, Associate Professor in Molecular Physiology. (lu.se)
Lund University1
- PhD in Molecular Medicine from Lund University, Sweden. (lu.se)
Analysis5
- Analysis of High Accuracy, Quantitative Proteomics Data in the MaxQB Database. (mpg.de)
- Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
- Proteomic analysis and discovery using affinity proteomics and mass spectrometry. (lu.se)
- His research utilises miniaturised bioanalytical techniques, lab-on-a-chip, where microchip based biomarker analysis and sample preparation in proteomics is in focus. (lu.se)
- Gregory LA, Aguissa-Toure AH, Pinaud N, Legrand P, Gleizes PE, Fribourg S. Molecular basis of Diamond-Blackfan anemia: structure and function analysis of RPS19. (medlineplus.gov)
Affinity1
- By employing this information, molecule libraries can be filtered for subsequent affinity assays and molecular structures can be refined with regard to affinity and selectivity. (uni-marburg.de)
Assays1
- Data, reagents, assays and merits of proteomics for SARS-CoV-2 research and testing. (cdc.gov)
Interactions1
- Ritchlin is co-leading a new research team dedicated to psoriatic spectrum diseases as part of the $64.5 million Autoimmune and Immune-Mediated Diseases Program (AIM) of the Accelerating Medicine Partnership (AMP), a huge public-private partnership involving the National Institutes of Health that is collecting and analyzing troves of biological data in order to better understand the cellular and molecular compositions and interactions that lead to disease. (medscape.com)
Genetics1
- Familiality and molecular genetics of attention networks in ADHD. (mpg.de)
Quantitative2
- Quantitative Interaction Proteomics and Genome-wide Profiling of Epigenetic Histone Marks and Their Readers. (mpg.de)
- MSQuant, an Open Source Platform for Mass Spectrometry-Based Quantitative Proteomics. (mpg.de)
Tissues1
- According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)
Cells1
- Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)
Proteases1
- Rapid SARS -CoV-2 Adaptation to Available Cellular Proteases. (helmholtz-hzi.de)
Transition1
- As part of its work, this eight-center project - coined ELLIPSS, for Elucidating the Landscape of Immunoendotypes in Psoriatic Skin and Synovium - hopes to define at a molecular and single-cell level how the transition to PsA unfolds in the setting of psoriasis. (medscape.com)
Centre1
- Inflammation and Immunity Group, Institute of Molecular Medicine, Trinity Centre for Health Sciences, St James' Hospital, Dublin 8, Ireland. (tcd.ie)
Study2
- In this study, proteomics and metabonomics techniques were used to analyze the tissue and plasma of DOX-induced heart failure (HF) in rats and to clarify the molecular mechanism of the harmful effects of DOX on cardiac metabolism and function in rats from a new point of view. (frontiersin.org)
- Global molecular diversity of RSV - the 'INFORM RSV' study. (cdc.gov)
Data3
- We scrutinized the proteomics data to acquire in-depth site-specific glycoproteomic profiles of the GBM subtypes that will assist in identifying specific glycosylation changes in GBM. (bu.edu)
- In bottom-up proteomics, data-dependent acquisition (DDA) selects precursors from full-scan MS 1 mass spectrum for fragmentation, generating tandem (MS/MS or MS 2 ) mass spectra that can be matched to spectra in a database. (elifesciences.org)
- fconv: Format Conversion, Manipulation and Feature Computation of Molecular Data. (uni-marburg.de)
Vitro1
- The studies use both in vitro cellular models of bacterial communities (biofilms) to characterise the phenotype of disease-causing bacteria. (lu.se)
Define1
- We are conducting next-generation sequencing and microarray studies involving Australian and international patient cohorts to define molecular signatures associated with tumour characteristics and outcome. (edu.au)
Investigations1
- Our investigations are performed at the cellular level but also at the whole organism level in advanced animal models of sepsis. (lu.se)
Medicine2
- EMBO Molecular Medicine , 14(3), e14764. (gla.ac.uk)
- Johan Malmström is Professor and principal investigator of the Infection Medicine Proteomics group at the Department of Clinical Sciences. (lu.se)
University1
- MSc in Molecular Medical Microbiology from University of Nottingham, the U.K. (lu.se)
Function1
- In addition we have recently started to explore the structure and molecular function of pain receptors from animals, i.e. transient receptor potential (TRP) ion channels, and their activation by temperature and various ligands. (lu.se)