Moiety binds polysaccharide carrier proteins. Medical search. Frequent questions

Recent advances in drug design have led to the development of several classes of novel therapeutic macromolecules including peptides, proteins, monoclonal antibodies, immunotoxins, lysozymes, plasmid DNA, antisense oligodeoxynucleotides, and short interfering RNA. (usc.edu)
Here we discuss two approaches currently used to investigate the global dynamics of phosphorylation based on the isolation of phosphorylated proteins or peptides. (acs.org)
Modified high activity binding peptides (mHABPs) were synthesised for complete full protection antimalarial vaccine development producing a large panel of individually fully protection-inducing protein structures (FPIPS) and very high long-lasting antibody-inducing (VHLLAI) mHABPs. (shengsci.com)

Polysaccharides are T-independent antigens, and can not be processed or presented on MHC molecules to interact with T-cells. (allindianpatents.com)
Conjugate vaccines in which polysaccharide antigens are covalently linked to carrier proteins belong to the most effective and safest vaccines against bacterial pathogens. (biomedcentral.com)
Two different periplasmic carrier proteins, AcrA from C. jejuni and a toxoid form of Pseudomonas aeruginosa exotoxin were glycosylated with Shigella O antigens in E. coli . (biomedcentral.com)
However, inclusion of protein antigens fromStreptococcus pneumoniaecombined with potent adjuvants and a suitable delivery system are expected to both extend protection to serotype strains not represented in the formulation and stimulate a broader immune response, thus more effective in young children, elderly, and immunocompromised populations. (univ-nantes.fr)

As proteo refers to protein, while glycan represents sugar or a group of sugars, so proteoglycan is a long polysaccharide chain covalently attached to a protein. (biochemistryconferences.com)

Pneumococcus is encapsulated with a chemically linked polysaccharide which confers serotype specificity. (allindianpatents.com)
pneumoniae serotype 14 capsular polysaccharide (CP14) linked to the Pneumococcal surface protein A (PsaA) using chitosan NPs (CNPs). (univ-nantes.fr)

Cysteine engineered anti-MUC16 antibodies (Ab) are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered anti-MUC16 antibody-drug conjugates having Formula I: Ab-(L-D)p I where p is 1 to 4. (justia.com)
Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)

Glycoside hydrolases cleave the glycosidic linkage between two carbohydrate moieties. (iastate.edu)
The carbohydrate moieties on HIV-1 envelope glycoprotein (Env) act as shields to mask conserved neutralizing epitopes, while the hyperimmunogenic variable regions are immunodominant in inducing non-neutralizing antibodies, representing the major challenge for using Env as a vaccine candidate to induce broadly neutrali. (shengsci.com)
The finding that, when the protein-polysaccharide complex (mainly chondroitin 4-sulfate) from pig rib-cartilage was treated with 0.2 M potassium hydroxide for 20 hours at 4°, only about 80% of the carbohydrate moieties were removed by /3-elimination, was interpreted as. (chempedia.info)
We are also focused on the development of chemoselective, convenient and mild synthetic strategy to modify the cysteine sulfhydryl group of peptide sequences in order to introduce different functionalities, such as carbohydrate moieties. (cnr.it)

Frequently used methods are based on, for example, antigen-antibody systems, nucleic acid hybridization techniques, and protein-ligand systems. (justia.com)
Strategies, which have been designed to overcome this lack of immunogenicity in infants, include the linking of the polysaccharide to large immunogenic proteins, which provide bystander T-cell help and which induce immunological memory against the polysaccharide antigen to which it is conjugated. (allindianpatents.com)
A nationwide vaccination programme in Italy against hepatitis B virus infection in infants of hepatitis B surface antigen-carrier mothers. (shengsci.com)

These promising results led us to develop a novel sulfated polysaccharide-based anionic siRNA delivery platform based on complexation of sulfated polysaccharide (such as alginate sulfate (AlgS)) with siRNA mediated by calcium ions [17] . (jims.org.il)
Table 7.2 Effect of the presence of an anionic polysaccharide on the measured zeta potential (Q of emulsion droplets stabilized by proteins under experimental conditions corresponding to protein-polysaccharide complexation. (chempedia.info)

In some organisms the capsular material appears to be a glycoprotein in others, a protein-polysaccharide complex in still others , a polysaccharide framework with the spaces filled in by a larger amount of glutamyl polypeptide. (chempedia.info)

Although lipids are a distinct class of biomolecules, we shall see that they often occur combined, either covalently or through weak bonds, with members of other classes of bio-molecules to yield hybrid molecules such as glycolipids , which contain both carbohydrate and lipid groups, "and lipoproteins , which contain both lipids and proteins. (medmuv.com)

An immunogenic composition comprising 2 S. pneumoniae recombinant proteins wherein one of the proteins is selected from the Poly Histidine Triad family (PhtX) at a dose of between 1-100 µg and optionally comprising an adjuvant. (allindianpatents.com)
Glycoconjugate synthesis, in particular expression of oligosaccharyltransferase PglB, strongly inhibited growth of E. coli cells after induction, making it necessary to separate biomass growth and recombinant protein expression phases. (biomedcentral.com)
The presented data demonstrate that glycosylated proteins can be produced in recombinant E. coli at a larger scale. (biomedcentral.com)

In an effort to develop an appropriate delivery system that will overcome these limitations and to improve the safety of potential siRNA -based therapeutics recent research has focused on biocompatible anionic carriers. (jims.org.il)

Carrier-linked prodrugs are for drugs with major drawbacks, which are linked to a nontoxic carrier or promoiety through covalent linkage to change or get rid of their undesirable physicochemical properties. (ijpsonline.com)

Fourier transform infrared spectroscopy (FT-IR) showed that the bio-carriers were composed of lignin moieties and polysaccharide units. (ncsu.edu)

Binding of all ligands by all three constructs is energetically favorable, enthalpy-driven, and subject to enthalpy-entropy compensation. (iastate.edu)
Furthermore, use of delivery systems allows surface modification of the nanoparticles (NPs) and incorporation of targeting ligands that bind specifically to receptors on target cells to induce receptor-mediated endocytosis and improve cellular uptake. (jims.org.il)
Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. (lookformedical.com)

Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (lookformedical.com)

In contrast to isolated bacterial polysaccharides, conjugate vaccines induce a long-lasting T-lymphocyte dependent immunological memory [ 4 , 5 ]. (biomedcentral.com)

Here we show that amino acid side-chains located in peptide binding region (PBR) positions p3 and p7 displayed specific electron charges and side-chain gauche(+) orientation for interacting with the TCR. (shengsci.com)
Evidence shows that conserved peptide 1815 from this protein having high red blood cell binding ability plays an important role in the invasion process. (shengsci.com)
In addition, our skills are employed for the design, synthesis and biological evaluation of new peptide analogues endowed with a good binding affinity toward biological targets involved in cancer illnesses, such as the CXCR4 and HER2 receptors. (cnr.it)
In addition, the controlled drug delivery area needs further development of techniques for delivery of peptide and protein drugs. (scialert.net)

An enclosed structure that transports proteins and other molecules around the cell or to the cell surface. (cdghub.com)

Cyanine dyes that are highly charged, such as those including multiple sulfonate, alkylsulfonate, carboxylate and/or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention. (justia.com)
The compounds of the invention are fluorophores that are derivatized to allow their facile attachment to another moiety. (justia.com)
The initially formed complexes with low- molecular-weight compounds are exchanged with intermolecular polysaccharide complexes, which cause delayed crosslinking. (chempedia.info)
B) protein-polysaccharide complex with amphiphilic compounds. (chempedia.info)

In this seminar, I will discuss our research efforts to develop "intelligent" pH-sensitive, membrane-destabilizing, and degradable polymeric carriers that can effectively deliver therapeutic nucleic acids past the endosomal membrane and into the cytoplasm of cancer cells to successfully suppress the expression of targeted genes. (usc.edu)

The present invention relates to a combination of 2 or more S. pneumoniae proteins, their manufacture and use in medicine as a vaccine. (allindianpatents.com)
In yet another related aspect is a method for making the vaccine of the invention by selecting and isolating 2 different S. pneumoniae proteins and mixing both proteins with a pharmaceutically acceptable carrier. (allindianpatents.com)

These can be inorganic (for example, water and metal ions) or organic (for example, the amino acids, which are used to synthesize proteins). (wikipedia.org)
Twenty alpha-amino acids are the subunits which are polymerized to form proteins. (lookformedical.com)
So the study of glycan structures is also complicated by the lack of a proper and direct template for their biosynthesis, contrary to the case with amino acids and proteins. (biochemistryconferences.com)
These phytosiderophores ("plant iron carriers") are built up from non-proteinogenic amino acids and play a key role in micronutrient uptake in gramineous plants. (tuwien.ac.at)

These prodrugs subsequently undergo enzymatic or non-enzymatic cleavage to release the active drug moiety (figure 1) [ 12 , 13 ]. (ijpsonline.com)

Though several studies have been carried out on various prodrugs, surprisingly very few reviews on different carriers, which can be used for prodrug synthesis were published to date. (ijpsonline.com)
In this review different types of carriers, which can be used for prodrug synthesis are summarized. (ijpsonline.com)
The objective of this review is to provide the researchers with a compilation of which carriers would be suitable for prodrug synthesis and what would be their benefits. (ijpsonline.com)
This study was performed to identify the carrier proteoglycan (PG) and the sulfotransferase gene involved in synthesis of the surface P-selectin-reactive CS-GAGs in human breast cancer cells with high metastatic capacity, as well as to determine a direct role for CS-GAGs in metastatic spread. (biomedcentral.com)
After induction glycoconjugates generally appeared later than unglycosylated carrier protein, suggesting that glycosylation was the rate-limiting step for synthesis of conjugate vaccines in E. coli . (biomedcentral.com)

The carrier proteoglycan CSPG4 was highly expressed on the aggressive breast cancer cell lines and contributed to the P-selectin binding and CS-A expression. (biomedcentral.com)

Several less common sugars are also covalently Unked to the polysaccharide complex. (chempedia.info)

The preferred route of administration for an injected therapeutic agent is subcutaneous (SC), but SC injections are generally limited to no more than 1-2 mL in volume, representing a major challenge, especially for large protein biologics. (pharmtech.com)

Large proteins are typically not suited for oral administration, as such drugs would be degraded in the digestive tract and poorly absorbed. (pharmtech.com)

Thorpe, (1985) "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review," in Monoclonal Antibodies '84: Biological And Clinical Applications, A. Pinchera et al (ed.s), pp. 475-506). (justia.com)

The calcium-siRNA nano-complexes (bearing only a mild negative surface charge) were stable in the presence of serum proteins and successfully induced a very high (~80%) level of gene silencing in several cell types [16] . (jims.org.il)
The receptors on the cell surface are normal surface components of the host, such as proteins, polysaccharides, or lipoprotein-polysaccharide complexes, to which the virus particle attaches. (chempedia.info)
V. Naturally Occurring Polysaccharide Complexes of Interest in Connection. (chempedia.info)
Protein-polysaccharide complexes and coacervates. (chempedia.info)
In a specific example , some W/O/W double emulsions with polyglycerol polyricinoleate (PGPR) as the primary emulsifier and WPI-polysaccharide complexes as the secondary emulsifying agent were found to be efficient storage carriers for sustained release of entrapped vitamin Bi (Benichou et al. (chempedia.info)
Structure and technofunctional properties of protein-polysaccharide complexes . (chempedia.info)
Emulsion stabilization by protein-polysaccharide complexes . (chempedia.info)
At the mixing ratio where coacervation is maximized, the protein-polysaccharide complexes are electrically neutral. (chempedia.info)
The expected greater size of protein-polysaccharide complexes can reduce the diffusion rate of the adsorbing species towards the interface. (chempedia.info)
In addition, Ganzevles and co-workers (2006) have suggested that the diffusion of protein in the complexes may not solely be responsible for the slow surface tension decay. (chempedia.info)
Rather, the gradual dissociation (and subsequent adsorption) of protein from complexes, when they are in close proximity to the interface, could also contribute to the behaviour. (chempedia.info)

In a preferred embodiment, one of the proteins is from the Poly Histidine Triad family (PhtX). (allindianpatents.com)
In another preferred embodiment, one of the proteins is from the Choline Binding Protein family (CbpX), or CbpX truncates, or CbpX truncate-LytX truncate chimeric proteins. (allindianpatents.com)

These polysaccharides, which comprise 20-35% of terrestrial biomass, present major challenges for the efficient microbial bioconversion of lignocellulosic feedstocks to fuels and other value-added products. (biomedcentral.com)

The presence of a particular analyte can often be determined by binding methods that exploit the high degree of specificity, which characterizes many biochemical and biological systems. (justia.com)

Preferred labels are inexpensive, safe, and capable of being attached efficiently to a wide variety of chemical, biochemical, and biological materials without significantly altering the important binding characteristics of those materials. (justia.com)

Emil Fischer, who studied the chemistry of proteins, and F. Gowland Hopkins, who studied enzymes and the dynamic nature of biochemistry, represent two examples of early biochemists. (wikipedia.org)
Les interactions sucres/protéines (enzymes, lectines) sont au cœur de la communication cellulaire tant extracellulaire (interactions cellule-cellule, cellule-matrice, hôte-pathogène) qu'intracellulaire (GlcNAcylation en tant que modification post-traductionnelle). (univ-nantes.fr)

It is of fundamental importance in determining PROTEIN CONFORMATION. (lookformedical.com)

A large number of cells adhered to the porous bio-carriers, and the cells of the experimental group to which the carriers were added exhibited higher metabolic activities. (ncsu.edu)

Las infecciones bacterianas con Escherichia coli productor de toxina Shiga (Stx) (STEC) están implicadas en el desarrollo del síndrome urémico hemolítico (SUH). (medicinabuenosaires.com)

It was found that efficiency of glycosylation but not carrier protein expression was highly susceptible to the physiological state at induction. (biomedcentral.com)

a: oligosaccharyltransferase PglB, b: carrier protein with signal sequence for secretion to the periplasm, c: undecaprenyl-pyrophosphate-linked polysaccharides. (biomedcentral.com)

Binding and thermodynamic properties of a carbohydrate binding module (CBM) and a glycoside hydrolase family 44 xyloglucanase/endoglucanase catalytic domain (CD) from Ruminococcus flavefaciens , both when separate and when linked to each other, have been quantified when binding various β-1,4-linked glucans and xylans. (iastate.edu)
Xylans are the most abundant noncellulosic polysaccharides in lignified secondary cell walls of woody dicots and in both primary and secondary cell walls of grasses. (biomedcentral.com)

These techniques are particularly suitable for developing environmentally sustainable and energy-efficient synthetic process to prepare biopolymer-based nano-sized material to be employed as drug delivery carrier systems. (cnr.it)

The processes are time-consuming and costly, and often large-scale cultivation of pathogenic bacteria is required for polysaccharide biosynthesis, making conjugate vaccines prohibitively expensive for vaccination campaigns in developing countries. (biomedcentral.com)

A large group of proteins that move substances across a membrane, e.g. as part of nutrient uptake or waste disposal. (cdghub.com)

Blocking the expression of CHST11 with siRNA inhibited CS-A expression and P-selectin binding to MDA-MB-231 cells. (biomedcentral.com)

In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an adaptor moiety. (justia.com)

The drug moieties may affect cytotoxic and cytostatic mechanisms including tubulin binding, DNA binding, or topoisomerase inhibition. (justia.com)

Bioprecursors do not have a promoiety or carrier but yield the active compound upon biotransformation. (ijpsonline.com)

It is used in protein biochemistry. (lookformedical.com)

Cell surface P-selectin binding depends on CHST11 gene expression. (biomedcentral.com)

The prodrug approach using various carriers has evolved as a significant tool in surmounting different obstacles in relation to drug formulation and targeting. (ijpsonline.com)
No. 4,975,278) allows targeted delivery of the drug moiety to tumors, and intracellular accumulation therein, where systemic administration of these unconjugated drug agents may result in unacceptable levels of toxicity to normal cells as well as the tumor cells sought to be eliminated (Baldwin et al (1986) Lancet pp. (justia.com)

The specific surface areas of the LCC-72/CNC and the LCC-48/CNC composite carriers were 24.2 m2/g and 28.9 m2/g, respectively, while their pore sizes were 28.6 nm and 33.6 nm, respectively. (ncsu.edu)
The specific surface areas of the LCC-72/CNC and the LCC-48/CNC composite carriers were 24.2 m 2 /g and 28.9 m 2 /g, respectively, while their pore sizes were 28.6 nm and 33.6 nm, respectively. (ncsu.edu)
Specific protein-binding measures are often used as assays in diagnostic assessments. (lookformedical.com)
An AMC is a legally-binding agreement for an amount of funds to subsidize the purchase, at a given price, of an as yet unavailable vaccine against a specific disease causing high morbidity and mortality in low-income countries. (who.int)
A subunit of a larger biomolecule, e.g. a single amino acid in a protein or a specific sugar in an oligosaccharide. (cdghub.com)
The virus particle itself has one or more proteins on the outside which interact with specific cell surface components called receptors. (chempedia.info)

These carriers have longer circulation time, less interaction with serum components, and lower cytotoxicity compared to cationic carriers. (jims.org.il)

In fact, if corn contains appreciable amounts of niacin, it is present in a bound form that is not nutritionally available to humans. (healthjade.net)

Carrier would change a drug's physical attributes in order to increase its fat or water solubility or to allow site-directed delivery. (ijpsonline.com)

L'équipe travaille à la fois sur la conception de lectines artificielles que d'inhibiteurs dérivés de sucres afin de les utiliser en tant qu'outils pour cntribuer à la compréhension de processus biologiques que pour des applications en santé (diagnostic, anti-infectieux, anti-cancéreux). (univ-nantes.fr)
An iron-binding plasma protein that is used a diagnostic biomarker for N-linked CDG. (cdghub.com)

Anatomy9

Organisms23

Diseases2

Chemicals and Drugs134

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes52

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science5

Health Care3

Peptides3

Antigens4

Covalently attached1

Serotype2

Conjugates2

Carbohydrate moieties4

Antigen3

Complexation2

Capsular1

Lipids1

Recombinant3

Anionic1

Covalent linkage1

Lignin1

Ligands4

Glycosidic linkage1

Induce1

Peptide4

Molecules1

Compounds4

Nucleic1

Pneumoniae2

Amino acids4

Cleavage1

Synthesis5

Proteoglycan1

Sugars1

Therapeutic1

Poorly1

Antibody1

Complexes11

Embodiment2

Comprise1

Specificity1

Chemical1

Enzymes2

Conformation1

Experimental1

Escherichia1

Glycosylation1

Sequence1

Xylans2

Synthetic1

Biosynthesis1

Substances1

SiRNA1

Invention1

Mechanisms1

Compound1

Biochemistry1

Gene1

Drug2

Specific6

Components1

Amounts1

Delivery1

Diagnostic2