SerineAmino Acid SequencePhosphorylationMolecular Sequence DataPhosphoserineBinding SitesProtein BindingCell LineMutagenesis, Site-DirectedProtein KinasesProtein-Serine-Threonine KinasesTyrosineMutationKineticsPhosphoproteinsBase SequenceEnzyme ActivationProtein Structure, TertiaryThreonineRecombinant ProteinsCarrier ProteinsSequence Homology, Amino AcidSerine EndopeptidasesCells, CulturedPeptide MappingProtein Kinase CDNA-Binding ProteinsTransfectionPhosphopeptidesSubstrate SpecificityModels, MolecularSignal TransductionCyclic AMP-Dependent Protein KinasesProtein Processing, Post-TranslationalRecombinant Fusion ProteinsElectrophoresis, Polyacrylamide GelBlotting, WesternAmino Acid SubstitutionPoly(A)-Binding ProteinsProtein ConformationPeptide FragmentsProtein-Tyrosine KinasesCasein Kinase IICloning, MolecularEnzyme InhibitorsCOS CellsHeLa CellsOxidative PhosphorylationStructure-Activity RelationshipMolecular WeightTranscription FactorsSequence AlignmentProteinsRNA, MessengerPhosphotyrosineEscherichia coliPhosphothreonineAmino AcidsPrecipitin TestsCalciumMembrane ProteinsAmino Acid MotifsPhosphoprotein PhosphatasesNuclear ProteinsCattleGene Expression RegulationPeptidesTranscription, GeneticCell MembraneProto-Oncogene Proteins c-aktIntracellular Signaling Peptides and ProteinsImmunoblottingCell NucleusMitogen-Activated Protein KinasesProto-Oncogene ProteinsImmunoprecipitationModels, BiologicalDNACell Line, TumorCysteineDNA PrimersPhosphoamino AcidsCalcium-Calmodulin-Dependent Protein KinasesTime FactorsSerine Proteinase InhibitorsTumor Cells, CulturedSerine ProteasesTrypsinProtein TransportProtein Structure, SecondaryAlanineAdenosine TriphosphateCricetinaeBacterial ProteinsRNA-Binding Proteins14-3-3 ProteinsCytoplasmTetradecanoylphorbol AcetateCasein KinasesAdaptor Proteins, Signal Transducing