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Hematopoietic Stem CellsHematopoietic Stem Cell TransplantationStem CellsAntigens, CD34Peripheral Blood Stem Cell TransplantationMice, SCIDMice, Inbred NODFetal BloodHematopoiesisHematopoietic Stem Cell MobilizationCell LineageCell DifferentiationCord Blood Stem Cell TransplantationInterleukin Receptor Common gamma SubunitColony-Forming Units AssayTransplantation, AutologousBone Marrow CellsStem Cell TransplantationTransplantation, HeterologousAntigens, CD38Transplantation, HomologousStem Cell FactorLentivirusEmbryonic Stem CellsGraft SurvivalAdult Stem CellsFlow CytometryBone MarrowAntigens, CDGranulocyte Colony-Stimulating FactorStem Cell NicheTransduction, GeneticCells, CulturedCell SeparationBone Marrow TransplantationTransplantation ConditioningTransplantation ChimeraPluripotent Stem CellsGraft vs Host DiseaseLeukapheresisADP-ribosyl CyclaseGenetic VectorsHematologic NeoplasmsCell ProliferationCell Culture TechniquesMultipotent Stem CellsAntigens, Thy-1Interleukin-3Induced Pluripotent Stem CellsNeural Stem CellsLymphopoiesisRetroviridaeBlood Component RemovalMesenchymal Stem Cell TransplantationMice, Inbred C57BLMesenchymal Stromal CellsCell DivisionGenetic TherapyImmunophenotypingTime FactorsCoculture TechniquesCell SurvivalMelphalanBusulfanStromal CellsCell CountWhole-Body IrradiationFetal Stem CellsBlood CellsTissue DonorsCombined Modality TherapyAntigens, CD45CyclophosphamideT-LymphocytesTreatment OutcomeLeukemiaGreen Fluorescent ProteinsBlood Cell CountMultiple MyelomaAntigens, DifferentiationGene Expression RegulationGene Transfer TechniquesCell CycleHematopoietic SystemHistocompatibilityCell LineMyeloablative AgonistsStem Cell ResearchMesonephrosHistocompatibility TestingMyelopoiesisNeoplastic Stem CellsProto-Oncogene Proteins c-kitAntineoplastic Combined Chemotherapy ProtocolsPlant StemsThrombopoietinLuminescent ProteinsK562 CellsRecurrenceThymus Gland
TransplantationDifferentiationVitroPluripotent stemMobilizationTissuesVivoEmbryonicImmuneProliferationAcuteCharacterizationProgenitor cellLineagesHSCsBiology2016Regenerative medicineGeneticEndothelial cellsPrecursorsSignaling pathwayAllogeneicTranscriptionalTumor cellsProgenitorsIsolationCytokinesProteinsInduceLineageMiceEffectorBiolCytokineHematopoiesisPathwaysStromal cellsPhenotypes2019Self-renewalTraining in pluripotentDifferentiateMononuclear cellsRegulationPatientsSubsetDendriticUmbilicalPrecursorGene expressionInteractionsAdultTumorsSuppressLeukemiasEndogenousFlow Cytometry2021LiverMechanismsAdditionallyTranscriptionMesenchymalFateMalignant
Transplantation15
  • Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
  • Craig A. Byersdorfer, MD, PhD , is a pediatric hematologist/oncologist in the Division of Blood and Marrow Transplantation and Cellular Therapies, with a research focus on understanding posttransplant complications, specifically graft-versus-host disease (GVHD) and the role that T cells play in driving this condition. (upmcphysicianresources.com)
  • If we can modulate T-cell metabolism in a way that shuts down the most active cells driving the condition, or identify metabolic ways to eliminate them altogether, we will make blood and marrow transplantation (BMT) a safer and more effective therapy for individuals with otherwise incurable diseases," says Dr. Byersdorfer. (upmcphysicianresources.com)
  • Handed out for either clinical or preclinical investigation of complications arising after allogeneic hematopoietic stem cell transplantation (alloHSCT), the award is administered over three years with a maximum support of $240,000. (upmcphysicianresources.com)
  • Previous research by Dr. Byersdorfer and his team has demonstrated that the cellular energy sensor, AMP-activated protein kinase (AMPK), plays a necessary role in T cells in GVHD models, and his belief is that modulating this pathway may result in a decreased incidence of GVHD following allogeneic transplantation in humans. (upmcphysicianresources.com)
  • MSCs were obtained from the bone marrow (BM) cells remaining in the bag and filter used in the transplantation of hematopoietic stem cells. (springer.com)
  • Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
  • 11 , 12 Compelling evidences have also showed that the gut microbiome could modulate alloimmunity and rejection, directly implicating the gut microbiome as a therapeutic target in organ transplantation. (nature.com)
  • Cytomegalovirus-Specific CD8+ T-Cells With Different T-Cell Receptor Affinities Segregate T-Cell Phenotypes and Correlate With Chronic Graft-Versus-Host Disease in Patients Post-Hematopoietic Stem Cell Transplantation. (ki.se)
  • In the 1960s, the discovery of bone marrow transplantation paved the way for further research into the potential medical applications of stem cells. (stemcellassurance.com)
  • Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
  • It should be administered under the supervision of a qualified physician who is experienced in allogeneic hematopoietic stem cell transplantation, the use of cancer chemotherapeutic drugs and the management of patients with severe pancytopenia. (nih.gov)
  • The pharmacokinetics of BUSULFEX were studied in 59 patients participating in a prospective trial of a BUSULFEX-cyclophosphamide preparatory regimen prior to allogeneic hematopoietic progenitor stem cell transplantation. (nih.gov)
  • The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
  • Treatment includes induction chemotherapy to achieve remission and postremission chemotherapy (with or without stem cell transplantation) to avoid relapse. (msdmanuals.com)
Differentiation35
  • Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)
  • Our findings reveal that during EHT, metabolism is a major regulator of primitive versus definitive hematopoietic differentiation. (lu.se)
  • Integrated transcriptomic and regulatory network analyses identify microRNA-200c as a novel repressor of human pluripotent stem cell-derived cardiomyocyte differentiation and maturation. (edu.hk)
  • TGF-β affects development and differentiation of human natural killer cell subsets. (sunnybrook.ca)
  • HEB-deficient T-cell precursors lose T-cell potential and adopt an alternative pathway of differentiation. (sunnybrook.ca)
  • The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
  • The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
  • Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
  • This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
  • 2016). Natural killer cell-based adoptive immunotherapy eradicates and drives differentiation of chemoresistant bladder cancer stem-like cells. (springer.com)
  • We found that, in the erythropoietin-induced, CD34-positive hematopoietic stem cell (HSC) differentiation system, knockdown of p19 INK4d delays terminal erythroid differentiation, inhibits erythroblast growth due to increased apoptosis, and leads to the generation of abnormally nucleated late-stage erythroblasts. (biomedcentral.com)
  • Cul4a targets p27 for degradation and regulates proliferation, cell cycle exit, and differentiation during erythropoiesis. (biomedcentral.com)
  • Global transcriptome analyses of human and murine terminal erythroid differentiation. (biomedcentral.com)
  • Using ATOs developed from different cell lines the trainee assessed the ability to support CD34+ differentiation. (ca.gov)
  • Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
  • Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
  • Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
  • Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
  • Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming. (unicyte.ch)
  • In particular, stem cells are highly sensitive to extracellular signals that play a critical role in mainten- ance of stem cell characteristics, differentiation, and interplay with somatic cells. (unicyte.ch)
  • Several other environmental factors including oxy- gen concentration and mechanical, metabolic, and bio- chemical conditions have been shown relevant in cell differentiation and have been reviewed extensively (Fig. 1) [3]. (unicyte.ch)
  • It is a heterogeneous disease, cellularly and molecularly, with an overarching theme of ectopic self-renewal and arrested differentiation potential at multiple levels of the hematopoietic tree ( 1 ). (frontiersin.org)
  • Akin to the normal hematopoietic system, leukemias are sustained by a small number of leukemia stem-like cells (LSC), which can be distinct from the normal hematopoietic stem cells (HSC) but also exhibit functional characteristics of self-renewal and (abnormal or hindered) differentiation, and are often quiescent ( 2 - 4 ). (frontiersin.org)
  • Differentiation: Stem cells can differentiate into specialized cell types, such as muscle cells, nerve cells, or blood cells, based on the signals they receive from their surrounding environment. (stemcellassurance.com)
  • Controlling the expansion and differentiation of stem and progenitor cells in vivo and in vitro is an important goal in regenerative medicine and for the development of patient-based, disease-relevant cellular models. (gatech.edu)
  • Next, I will describe some work on the differentiation of neural precursor cells isolated from human olfactory epithelium (OE). (gatech.edu)
  • The results indicated that GSK-3 inhibition promotes the early stages of neuronal differentiation and decrease proliferation in cultures of adult human neural precursors. (gatech.edu)
  • Researchers have discovered a novel pathway based on Notch stimulation very early during the in-vitro differentiation that robustly support the emergence of T and NK cells from human induced Pluripotent Stem Cells (iPSC). (sciencedaily.com)
  • Now, researchers from Boston University Chobanian & Avedisian School of Medicine and Boston Medical Center have discovered a novel pathway based on Notch stimulation (a pathway involved in cell fate decisions, proliferation and death) very early during the in-vitro differentiation that robustly support the emergence of T and NK cells from human induced Pluripotent Stem Cells (iPSC). (sciencedaily.com)
  • In previously reports , Plasmodium vivax was found to be able to directly inhibited erythroid cell proliferation and differentiation. (biomedcentral.com)
  • Moreover, in vitro cultures of erythroid cells derived from haematopoietic stem cells has demonstrated that P. vivax is able to directly inhibit erythroid cell proliferation and differentiation [ 22 ]. (biomedcentral.com)
  • Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
  • They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
  • Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
  • Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
Vitro13
  • In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS differentially skews the hematopoietic output of HE cells toward either an erythroid fate with primitive phenotype, or a definitive lymphoid fate, respectively. (lu.se)
  • Notch signals are required for in vitro but not in vivo maintenance of human hematopoietic stem cells and delay the appearance of multipotent progenitors. (sunnybrook.ca)
  • Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature. (sunnybrook.ca)
  • The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. (springer.com)
  • One trainee focused on developing in vitro growth conditions to optimize expansion of luminal epithelial cells and c-KIT+ progenitor cells to explore the early stages of breast cancer. (ca.gov)
  • We particularly study Epstein-Barr Virus (EBV)-infection, which can also be used as a model to study early steps in in vitro transformation, viral genetic switches, non-genetic variation and cancer cell metabolism. (ki.se)
  • The EBV cell line models allow comparison of non-malignant versus malignant phenotypes of human cancer in vitro (lymphoblastoid cell lines, LCL vs Burkitt´s lymphoma cell line, BL). (ki.se)
  • Description: Agonist activity determined in vitro, measuring calcium response in CHO-K1 cells expressing hGPR15 and Aequorin. (guidetopharmacology.org)
  • We examined the impact of changes in glycogen synthase kinase-3 (GSK-3) activity on the fate of adult human OE neural precursors in vitro. (gatech.edu)
  • Recent advances in immunotherapy for cancer have stimulated a plethora of studies aimed at developing T cells and Natural Killer (NK) cells in-vitro from pluripotent stem cells. (sciencedaily.com)
  • Recent advances in immunotherapy for cancer have stimulated a plethora of studies aimed at developing T cells (white blood cells) and Natural Killer (NK) cells (immune cells with enzymes that can kill tumor cells or cells infected with a virus) in-vitro from pluripotent stem cells -- cells that are able to self-renew by dividing and developing into the three primary groups of cells that make up a human body. (sciencedaily.com)
  • Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
  • Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
Pluripotent stem8
Mobilization4
  • In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
  • The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
  • In many cases, successfully mobilization is not effected in the patient and inadequate numbers of hematopoietic stem cells are harvested from these patients using current methods. (justia.com)
  • Plerixafor use in autologous hematopoietic stem cell mobilization: Experience from a single center in Southern India. (edu.in)
Tissues13
  • It is ubiquitously expressed in many tissues and cell types. (wikipedia.org)
  • LNK is widely expressed in human tissues, with the highest expression in hematopoietic cells. (wikipedia.org)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
  • Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
  • Apigenin served as a potent therapy against UC in C57BL/6 mice through the inhibition of inflammatory cytokines,and COX-2, and through the reduction in immune cell infiltration in colon tissues [ 11 ] . (encyclopedia.pub)
  • Adult stem cells: These stem cells are found in various tissues and organs of the body, such as bone marrow, blood, and adipose tissue. (stemcellassurance.com)
  • Plasticity: Some types of stem cells have the ability to differentiate into cell types from other tissues or organs, a property known as plasticity. (stemcellassurance.com)
  • The history of stem cell research dates back to the 19th century, when scientists first observed that certain cells have the ability to regenerate and repair tissues. (stemcellassurance.com)
  • Stem cells serve as a valuable tool for studying the development and function of various organs and tissues, as well as for modeling diseases and developing new drugs. (stemcellassurance.com)
  • But research progressed and different trials explore the clinical potential of human MSCs isolated from bone marrow but also from other tissues including adipose tissue. (hindawi.com)
  • Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
  • In this investigation we sought to answer the question concerning the presence of mast cells in human and rat dental pulp tissues, under normal and inflammatory conditions. (bvsalud.org)
Vivo8
  • Shaping of natural killer cell antitumor activity by ex vivo cultivation. (springer.com)
  • T cells are modified ex vivo by transduction with viral vectors containing CAR or other modifications of interest. (bdbiosciences.com)
  • Recently, we developed a hematopoietic stem cell-based approach to tackle this issue, in which we can genetically alter nucleated hematopoietic precursor cells and differentiate them ex-vivo to mature erythrocytes that can be infected by P. falciparum. (stanford.edu)
  • NOTCH1 is known to be required for the production of hematopoietic progenitor cells (an intermediate cell type in blood cell development) with T cell potential in vivo. (sciencedaily.com)
  • Our strategy of modulating senescence response in vivo is to manipulate the expression of key senescence regulators identified in our genetic screen studies. (umassmed.edu)
  • The mouse models we have generated to modulate senescence response in vivo are also used in our studies of senescence regulation in aging, with an emphasis on adult stem cells. (umassmed.edu)
  • MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
  • In addition there is a lack of information regarding the presence of mast cells in dental pulp of rats, which are animals used to study important aspects of the pathophysiology of dental pulp in vivo 6 . (bvsalud.org)
Embryonic14
Immune9
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • As an outlook, we aim to develop personalized anti-aging clinical interventions that will attenuate aging-driven immune remodeling and hematopoietic disorders. (uni-ulm.de)
  • The long term objective of these studies is to better understand local immune mechanisms within the liver that modulate anti-viral defense and inflammation. (twincore.de)
  • Researchers at the University of California, Davis have developed a customizable polysaccharide that can be added to nanoparticles to reduce their rejection by the human immune system. (universityofcalifornia.edu)
  • First, we are interested in Epstein Barr Virus-mediated mechanisms of immune evasion with particular emphasis on pathways that promote survival and proliferation of EBV B cell lymphomas, the characterization of the human T cell and NK cell response to EBV and the identification of novel therapeutics for treatment of EBV B cell lymphomas. (stanford.edu)
  • Several studies have countered the notion that these three homeostatic systems in the body, nervous system, endocrine system, and immune system, function independent of each other and provided evidence for the bidirectional communication between these systems to regulate health and disease in humans. (brainimmune.com)
  • Cytokines and other factors produced by cells of the immune system can cross the blood-brain barrier to induce the release of neurotransmitters and hormones from the brain and pituitary, leading to several central nervous system (CNS)-dependent responses such as sleep, depression, thermogenesis, and anorexia [2-4]. (brainimmune.com)
  • Numerous studies from our laboratory and others have demonstrated that noradrenergic (NA) and peptidergic nerve fibers are present in various compartments of primary (bone marrow, thymus) and secondary (spleen, lymph nodes) lymphoid organs and that these nerve fibers can modulate immune functions [5-10]. (brainimmune.com)
  • During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
Proliferation13
  • MicroRNA miR-199a-3p regulates cell proliferation and survival by targeting caveolin-2. (sunnybrook.ca)
  • NK cell proliferation, phenotypic expression and cytotoxic activity were evaluated. (springer.com)
  • Polypyrimidine tract-binding protein induces p19(ink4d) expression and inhibits the proliferation of h1299 cells. (biomedcentral.com)
  • P19ink4d is a tumor suppressor and controls pituitary anterior lobe cell proliferation. (biomedcentral.com)
  • We are using this virus to study the initiating immortalizing process and switches between resting and proliferating phenotypes to better understand EBV protein - host cell interactions involved in tumorigenesis controlling the switch from cell arrest to proliferation and reprogramming of metabolism. (ki.se)
  • Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
  • To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
  • This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
  • By limiting cell proliferation, senescence impedes the accumulation of multiple mutations that are necessary for tumorigenesis. (umassmed.edu)
  • Furthermore, senescence activated by aberrant oncogenic activation, DNA damage or oxidative stress provides a failsafe mechanism that prevents proliferation of cells at risk of neoplastic transformation. (umassmed.edu)
  • However, cell proliferation is critical for renewal, repair and regeneration to maintain normal tissue homeostasis and functions. (umassmed.edu)
  • By limiting cell proliferation and consequently depleting the renewal capacity of stem or progenitor cells, senescence is thought to contribute to the aging process. (umassmed.edu)
  • In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)
Acute11
  • Moreover, co-expression of the interleukin-7 receptor together with LNK was carefully studied, and it was concluded that interleukin-7 receptor expression was significantly more highly expressed than LNK in B-cell acute leukemic lymphoma. (wikipedia.org)
  • This observation distinguished a novel subset of high-risk B-cell acute lymphoblastic lymphoma with a potential therapy targeting the interleukin-7 signaling pathway. (wikipedia.org)
  • Another study indicated that LNK can suppress the interleukin-7/JAK/STAT signaling pathway to restrict pre B-cell progenitor expansion and leukemia development, which provided a pathogenic mechanism and a potential therapeutic approach for B-cell acute lymphoblastic leukemia with SH2B3 gene mutations. (wikipedia.org)
  • Inhibition of notch signaling by gamma secretase inhibitor engages the rb pathway and elicits cell cycle exit in t-cell acute lymphoblastic leukemia cells. (biomedcentral.com)
  • Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
  • PEGylated G-CSF (BBT-015), GM-CSF (BBT-007), and IL-11 (BBT-059) analogs enhance survival and hematopoietic cell recovery in a mouse model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)
  • Long-term hematopoietic stem cell damage in a murine model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)
  • Establishing a murine model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)
  • Acute myeloid leukemia is a hematopoietic neoplasm of dismal prognosis that results from the accumulation of immature myeloid blasts in the bone marrow and the peripheral blood. (frontiersin.org)
  • CAR-T cells, which incorporate an antibody-derived extracellular receptor and T cell derived intracellular signaling domains, have shown convincing outcomes in certain types of leukemia and lymphoma, including commercial licensure of CD19 CAR-Ts for the treatment of relapsed/ refractory large B cell lymphoma and acute lymphoblastic leukemia. (bdbiosciences.com)
  • The technique has also shown preliminarily promising outcomes in B cell acute lymphoblastic leukemia (B-ALL), B cell non-Hodgkin's lymphoma (B-NHL), chronic lymphocytic leukemia (CLL), Hodgkin's lymphoma and multiple myeloma in clinical trials targeting CD19, CD20, CD22, CD30 and B-cell maturation antigen (BCMA), with many more under investigation. (bdbiosciences.com)
Characterization3
  • Isolation, expansion, and characterization of natural killer cells and their precursors as a tool to study cancer immunosurveillance. (springer.com)
  • A second trainee focused on developing a fluorescent marker to allow for the characterization of a CAR-T cell population as it interacts with the targeted brain tumor. (ca.gov)
  • Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis. (ox.ac.uk)
Progenitor cell4
  • In addition to its role in progenitor cell growth and commitment, LNK appears to be involved in cell motility and cellular interactions. (wikipedia.org)
  • The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
  • The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
  • In this seminar, I will first describe our investigation of the mechanisms responsible for the combinatorial cytokine dependence of stem/progenitor cell fate decisions. (gatech.edu)
Lineages3
  • AML is an aggressive malignancy of the non-lymphoid lineages of the blood. (frontiersin.org)
  • These neural precursor cells can be easily harvested from a minimally invasive nasal biopsy, making them a valuable cell source to study human neural cell lineages in health and disease. (gatech.edu)
  • Our ongoing studies have found that LT-HSC population, which give rise to all lineages of blood cells, increases in Smurf2-deficient mice, suggesting a beneficial effect of Smurf2 deficiency. (umassmed.edu)
HSCs8
  • Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). (lu.se)
  • However, metabolic processes underlying the emergence of HSCs from HE cells remain unclear. (lu.se)
  • The project will investigate the age-related functional decline of hematopoiesis and dissect the role of Cdc42 in the rejuvenation of Human Hematopoietic Stem Cells (HSCs). (uni-ulm.de)
  • It will additionally focus on, using single-cell sequencing and deep learning approaches, the transcriptional and mutational heterogeneity of HSCs upon aging. (uni-ulm.de)
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
  • Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
  • Although hematopoietic stem cells (HSCs) are useful in a variety of treatments, HSC donation is a difficult procedure. (universityofcalifornia.edu)
Biology10
  • In Systems Genetics , Methods in Molecular Biology 1488:75-120 [An updated primer in using GeneNetwork for molecular analysis of mouse and human cohorts. (genenetwork.org)
  • Cell cycle gene expression networks discovered using systems biology: Significance in carcinogenesis. (genenetwork.org)
  • International Journal of Biochemistry & Cell Biology, 40 , 2335-2340. (springer.com)
  • Natural killer cell biology: An update and future directions. (springer.com)
  • Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
  • MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
  • The relevance of these phenomena in stem cell biology and tissue repair is discussed. (unicyte.ch)
  • Building off experience in hematopoietic stem cell transplant and exploiting advances in genetic engineering, genome editing, immunology, immunotherapy, stem cell biology, understanding disease mechanisms and cell therapy manufacturing, considerable progress has been made with hundreds of clinical trials underway. (bdbiosciences.com)
  • Her research is focused on understanding how host factors from the human erythrocyte influence the biology and pathogenesis of the malaria parasite Plasmodium falciparum. (stanford.edu)
  • He received postdoctoral training at Stanford University School of Medicine, and joined the faculty in the Department of Cell Biology at University of Massachusetts Medical School in 2006. (umassmed.edu)
20164
  • 2016). More recently we found that during the early phase of MCMV infection IFN-β responses are induced in Kupffer cells within the liver. (twincore.de)
  • Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
  • Cell Stem Cell 19 , 569-572 (2016). (nature.com)
  • The peripheral blood mononuclear cells (PBMCs) have a round nucleus ( Delves, 2016 ). (frontiersin.org)
Regenerative medicine2
Genetic14
  • In humans, genetic linkage analyses, genome-wide association studies of single nucleotide polymorphisms, copy number variation surveys, and mutation screenings found the human chromosomal 12q24 locus, with the SH2B3 gene at its core, to be associated with an exceptionally wide spectrum of disease susceptibilities. (wikipedia.org)
  • Harenza JL, Muldoon PP, De Biasi M, Damaj MI, Miles MF (2014) Genetic variation within the Chrna7 gene modulates nicotine reward-like phenotypes in mice. (genenetwork.org)
  • First, we are elucidating the genetic determinants and disease relevance of ferroptosis, a newly recognized form of cell death. (duke.edu)
  • Recently, we have found that the genetic removal of MESH1 in tumor cells triggers extensive transcriptional changes and confers protection against oxidative stress-induced ferroptosis (11). (duke.edu)
  • Considerable effort over the last decade has been directed towards developing integrated and scalable single cell genetic analysis on chip (7, 8). (justia.com)
  • Solid tumors, autoimmune diseases, graft versus host disease (GvHD), genetic diseases and neurodegenerative diseases are investigated using many types of autologous and allogeneic cells with rapidly evolving cell engineering and editing approaches. (bdbiosciences.com)
  • A team of researchers led by Dr. Cristian Bellodi recently discovered a hardwired genetic control mechanism modulating individual spliceosomal components, known as splicing factors, in cells harboring oncogenic lesions common in human cancers. (lu.se)
  • This can also be achieved by directly modulating the activity of signaling enzymes or transcription factors using genetic engineering strategies or by delivering bioactive molecules in the cell cytosol or nucleus. (gatech.edu)
  • However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
  • In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
  • As a postdoc in Manoj Duraisingh's lab at Harvard School of Public Health she performed a genetic screen to identify critical host factors for Plasmodium falciparum malaria using red blood cells derived from hematopoietic stem cells. (stanford.edu)
  • Population genetic studies dating back to the mid-20th century first proposed that erythrocytes (red blood cells), the host cell for P. falciparum, have been under natural selection due to malaria. (stanford.edu)
  • Although the notion that malaria has helped shape the human genome is well- accepted, the lack of a nucleus in human erythrocytes has hindered our ability to study genetic interactions between these unusual host cells and P. falciparum parasites. (stanford.edu)
  • Olariu V, Manesso E, commitment and Peterson C. 2017 A deterministic method for estimating free energy genetic network reprogramming paths landscapes with applications to cell commitment and reprogramming paths. (lu.se)
Endothelial cells3
  • LNK is a negative regulator of signaling in endothelial cells, such as the TNF signaling pathway, especially in inflammation. (wikipedia.org)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Nevertheless, also in the absence of cell-free virus, i.e., upon co-culture of infected epithelial/endothelial cells with monocyte-derived macrophages (moMΦ) or dendritic cells (moDC), antiviral responses are induced that limit HCMV spread (Becker et al. (twincore.de)
Precursors3
  • Enforcement of γδ-lineage commitment by the pre-T-cell receptor in precursors with weak γδ-TCR signals. (sunnybrook.ca)
  • Developmental progression of fetal HEB(-/-) precursors to the pre-T-cell stage is restored by HEBAlt. (sunnybrook.ca)
  • HEBAlt enhances the T-cell potential of fetal myeloid-biased precursors. (sunnybrook.ca)
Signaling pathway1
  • The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found 16 significant regulated pathways in imDCs and 10 significant regulated pathways in mDCs, including the phagosome, cell adhesion signaling pathway, and inflammatory mediator regulation of TRP channels pathway. (frontiersin.org)
Allogeneic2
  • Allogeneic transfusion of whole blood and fractionated blood components remains a controversial topic with respect to transfusion triggers and practices. (medscape.com)
  • Technology making the transfusion of allogeneic blood products feasible includes Karl Landsteiner's landmark identification of the human blood groups A, B, and O in 1901. (medscape.com)
Transcriptional5
  • In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
  • Unexpected role for p19ink4d in post-transcriptional regulation of gata1 and modulation of human terminal erythropoiesis. (biomedcentral.com)
  • One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
  • Growing evidence suggests that transcriptional regulators and secreted RNA molecules encapsulated within membrane vesicles modify the phenotype of target cells. (unicyte.ch)
  • Validation using transcriptional and transgenic assays confirmed direct regulatory interactions consistent with a regulatory triad in immature blood stem cells, where Gata2 may function to modulate cross-inhibition between Gfi1 and Gfi1b. (ox.ac.uk)
Tumor cells3
  • Researchers at the University of California, Davis have developed a self-assembling, fibrous photosensitizer that targets mitochondria in tumor cells for destruction via photodynamic therapy with enhanced localization and potency. (universityofcalifornia.edu)
  • Patient PBMCs are collected by leukapheresis followed by enrichment approaches to isolate T cells while removing other cells such as B cells, monocytes, neutrophils, dendritic cells and contaminating tumor cells. (bdbiosciences.com)
  • Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
Progenitors1
  • We have identify a critical window when Notch activation robustly improves access to definitive intermediate cell type in blood cell development (hematopoietic progenitors) with T/NK cell lineage potential. (sciencedaily.com)
Isolation4
  • Microfluidic devices employing active valving to position and isolate cells have allowed for the isolation and genome amplification of individual microbial cells (32). (justia.com)
  • Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
  • Although these systems provide a high-throughput qPCR readout, they do not address the front end sample preparation and require single cell isolation by FACS or micropipette followed by off-chip processing and pre-amplification of starting template prior to analysis. (justia.com)
  • We assayed semen for DENV using reverse transcription PCR and searched for DENV RNA by virus isolation in Vero E6 cell cultures. (cdc.gov)
Cytokines8
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Dr. Byersdorfer's new award will be used to investigate the mechanism of AMPK's action in post-transplant T cells and determine the relationship by which AMPK is linked to pro-inflammatory cytokines like IL-6. (upmcphysicianresources.com)
  • The fourth generation of CAR-T design attempts to deliver cytokines to modulate the tumor microenvironment either by activating host effector T cells or hampering host suppressors and reinforcing memory T cells. (bdbiosciences.com)
  • These experimental cytokine-producing CAR-Ts, called T cells redirected for universal cytokine killing (TRUCKs), can deliver a variety of cytokines, such as IL-12, IL-15, IL-18 or IL-21. (bdbiosciences.com)
  • This usually achieved by exposing stem and progenitor cells to specific types and doses of cytokines, growth factors and extracellular matrix proteins. (gatech.edu)
  • T helper (Th) 2 cells, which recruit and accumulate in the lungs and produce a range of different effector cytokines. (cdc.gov)
  • However, more recent studies have revealed the potential collaboration of other helper T cells and their cytokines in this process. (cdc.gov)
  • The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)
Proteins5
  • R4 RGS proteins suppress engraftment of human hematopoietic stem/progenitor cells by modulating SDF-1/CXCR4 signaling. (edu.hk)
  • hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
  • These findings suggest that parasite proteins are able to inhibit erythroid cell growth by down-regulation of ezrin phosphorylation, leading to ineffective erythropoiesis ultimately resulting in severe malarial anaemia. (biomedcentral.com)
  • Many molecular pathways in eukaryotic cells are modulated by specific signaling proteins that are controlled, by phosphorylation and dephosphorylation, through the activity of kinase and phosphatase enzymes. (biomedcentral.com)
  • The regulation of complex and dynamic signal transduction proteins contributes to the destination of targeting proteins and the signal transduction of cell growth, and exposure to parasites can also influence signaling pathways. (biomedcentral.com)
Induce2
  • During erythropoiesis, the cells start to produce hemoglobin, lose nuclei and organelles, and induce plasma membrane remodeling. (elifesciences.org)
  • Membrane vesicles, actively released by cells, represent a mechanism of intercellular communication that is conserved evolutionarily and involves the transfer of molecules able to induce epigenetic changes in recipient cells. (unicyte.ch)
Lineage2
  • Context-dependent regulation of hematopoietic lineage choice by HEBAlt. (sunnybrook.ca)
  • or movements in a free energy landscape such that lineage choices are paths between stable cell states. (lu.se)
Mice11
  • Evaluation of heritable determinants of blood and brain serotonin homeostasis using recombinant inbred mice. (genenetwork.org)
  • We are investigating their function using culture cells, MESH1 KO mice, and other model organisms. (duke.edu)
  • For this, structural and functional consequences at synaptic interfaces built up by neurons and different types of glia cells in young and old mice will be characterized using conditional transgenic systems mimicking brain inflammaging and allowing cell-type-specific Notch/NF-kB modulation. (uni-ulm.de)
  • We found that upon Coxsackievirus B3 (CVB3) infection of mice hepatocytes are key innate effector cells that mount protective type I IFN responses. (twincore.de)
  • In conditional mice devoid of type I IFN receptor signaling selectively on hepatocytes, CVB3 infection is not controlled and the virus disseminates similarly as in mice devoid of the type I IFN receptor in all cells (Koestner et al. (twincore.de)
  • Specifically, the trainee looked at the ability of an endothelial cell AGO 1 gene knockout to rescue streptozotocin-induced damage in female mice. (ca.gov)
  • Mechanistically, we identified dysregulated erythropoiesis with reduced numbers of reticulocytes, the preferred host cells of P. yoelii , in Ac-deficient mice. (elifesciences.org)
  • In aged mice, the self-renewal capacity of long-term hematopoietic stem cells (LT-HSC) diminishes. (umassmed.edu)
  • The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
  • Castration of normal male mice induces expansion of the bone marrow B cell population, an effect that can be reversed by androgen replacement. (jci.org)
  • Androgens only affected B cell development in chimeric mice with androgen-sensitive stromal cells. (jci.org)
Effector2
  • Our first trainee focused on the impact of pseudouridine in coding and noncoding RNAs as an effector of glioblastoma stem cells (GSC) and the development of glioblastoma multiforme brain tumors. (ca.gov)
  • At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
Biol2
Cytokine5
  • LNK modulates crosstalk between integrin- and cytokine-mediated signals, thus controlling thrombopoiesis. (wikipedia.org)
  • We seek to further clarify the relationship between AMPK, metabolic stress, and cytokine sensitivity, and to translate these findings into human T cells. (upmcphysicianresources.com)
  • We hypothesized that combinatorial cytokine dependence arises in cell subsets in unique transitional stages where cell fate decision can be regulated by combinations of early- and late-acting factors. (gatech.edu)
  • We used adult bone marrow erythropoiesis (red blood cell development) as a model system since it depends on the sequential action of stem cell factor (SCF), an early-acting cytokine and erythropoietin (EPO), a late-acting cytokine. (gatech.edu)
  • Modulating the cytokine network in thickening of the basement membrane underlying the respiratory diseases such as asthma, with expected airway epithelium. (cdc.gov)
Hematopoiesis1
Pathways3
  • LNK negatively controls the activation of several receptors, including stem cell factor receptor (c-kit), thrombopoietin receptor (MPL), erythropoietin receptor (EPOR), platelet-derived growth factor receptor (PDGFR), macrophage colony-stimulating factor receptor (c-Fms), and their related pathways. (wikipedia.org)
  • A central focus of Dr. Byersdorfer's research is how T cell metabolism and the targeting of metabolic pathways, may be used to help limit, or even eliminate, GVHD post-transplant. (upmcphysicianresources.com)
  • Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
Stromal cells5
  • However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
  • Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
  • Immature B cells from normal mouse bone marrow were not responsive to the suppressive effect of androgens unless they were cocultured with marrow stromal cells or with supernatants from androgen-treated stromal cells, suggesting that the androgen effects are exerted through marrow stromal elements by production of a diffusible mediator. (jci.org)
  • Further experiments revealed that bone marrow stromal cells produced TGF-β in response to dihydrotestosterone (DHT), and neutralization of TGF-β in the DHT-treated stromal cells reversed the suppressive effects. (jci.org)
  • These experiments suggest that effects of androgens on developing B cells are mediated through androgen receptors in bone marrow stromal cells. (jci.org)
Phenotypes2
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • A common theme in cell therapy is the need to characterize and understand cell phenotypes and heterogeneity at every step of the process. (bdbiosciences.com)
20194
  • 2019). Interestingly, hepatocyte-derived MCMV does not infect other cells of the body until day 5 of infection, whereas from day 8 on hepatocyte-derived MCMV disseminates to the salivary gland (Tegtmeyer et al. (twincore.de)
  • 2019). This dissemination is dependent on the viral component MCK2 and is mediated by MCMV infected myeloid cells. (twincore.de)
  • 2019) is applied to develop improved methods for the generation of HCMV-specific T cell products for the use in patients with severe HCMV infection (Forschergruppe 2830, P04). (twincore.de)
  • Cossarizza A, ... Rahmig S, ... Waskow C, ... 2019 Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition), Eur J Immunol , 49(10):1457-1973. (waskowlab.com)
Self-renewal4
  • True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
  • Self-renewal: Stem cells have the ability to divide and produce more stem cells, ensuring a constant supply of undifferentiated cells. (stemcellassurance.com)
  • To qualify as stem cells, self-renewal has to be shown as well [ 10 ]. (hindawi.com)
  • Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
Training in pluripotent1
  • She then received a prestigious Commonwealth Scholarship for her DPhil research in the University of Oxford to study the molecular mechanisms of Duchenne Muscular Dystrophy, and was also a recipient of the American Heart Association Postdoctoral Fellowship in the University of California, San Francisco where she first started her training in pluripotent stem cell technology. (edu.hk)
Differentiate4
  • Currently it is analyzed how infiltrated myeloid cells differentiate to cells that are reminiscent of Kupffer cells within the liver. (twincore.de)
  • However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
  • Stem cells are defined as undifferentiated cells that can differentiate into specialized cell types and can divide to produce more stem cells. (stemcellassurance.com)
  • They are considered pluripotent, meaning they can differentiate into almost any cell type. (stemcellassurance.com)
Mononuclear cells1
  • The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)
Regulation8
  • The overexpression of LNK led to the inhibition of anti-CD3 mediated NF-AT-Luc activation, indicating that LNK is involved in the mechanism of T cell-negative regulation. (wikipedia.org)
  • As reported in our article recently published in Blood entitled "Unexpected roles for p19 INK4d in posttranscriptional regulation of GATA1 and modulation of human terminal erythropoiesis" [ 3 ], we demonstrated what roles p19 INK4d plays in human terminal erythropoiesis. (biomedcentral.com)
  • However, as shown in our study, p19 INK4d played important roles independent of cell cycle regulation, and the lack of cell cycle change was probably due to the compensatory up-regulation of p18 INK4c following p19 INK4d knockdown. (biomedcentral.com)
  • Our findings provide greater understanding of the role that CDKIs play in cell cycle regulation. (biomedcentral.com)
  • RNA splicing is a major nexus of gene expression regulation, shaping cellular identity during development, frequently altered in human cancers. (lu.se)
  • Using these 'senescence' mouse models, combined with different tumorigenesis models (for instance, p53 knockout, B cell lymphoma, skin cancer, colon cancer and liver cancer models), we hope to gain a better understanding of the regulation and contribution of senescence in tumor development. (umassmed.edu)
  • In this study, a phosphoproteomic approach was performed to dissect the molecular mechanism of phosphoprotein regulation, which is involved in the inhibitory effect of parasites on erythroid cell development. (biomedcentral.com)
  • This result suggests that phospho-ezrin is important for actin cytoskeleton regulation during erythroid cell growth and division. (biomedcentral.com)
Patients6
  • Furthermore, we examine SPC numbers in peripheral blood of polytraumatized patients. (geigerlab.com)
  • Our final student worked to introduce a "suicide gene" construct into engineered in iPSCs for the generation of the insulin-producing -cell to treat type 1 diabetes patients. (ca.gov)
  • METHODS: A total of 222 E. coli blood isolates were prospectively collected from patients with HCA-BUO from 12 tertiary-care hospitals in Spain (2017-19). (bvsalud.org)
  • Prediction of improved survival in patients with pancreatic cancer via IL-21 enhanced detection of mesothelin epitope-reactive T-cell responses. (ki.se)
  • PBMCs were separately isolated from the human peripheral blood of patients with AR and normal person (NP). (frontiersin.org)
  • When germ-free cell cultures became a laboratory routine, hopes were high for using this novel technology for treatment of diseases or replacement of cells in patients suffering from injury, inflammation, or cancer or even refreshing cells in the elderly. (hindawi.com)
Subset2
  • We have identified a subset of hematopoietic progenitor cells which fate decisions depend on both the SCF and EPO signals. (gatech.edu)
  • Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)
Dendritic3
  • 2018). This observation together with our recently improved understanding of antigen presentation by human dendritic cells (Döring et al. (twincore.de)
  • By applying single cell sequencing to HCMV infected human dendritic cells we aim to elucidate the delicate balance between the host response and evasion strategies deployed by HCMV. (twincore.de)
  • Dendritic cells (DCs), which are the most important antigen-presenting cells (APCs) that send signals to the T cells, mainly participate in the pathogenesis of many diseases with immunoregulatory mechanisms, such as AR. (frontiersin.org)
Umbilical1
  • Our third trainee in this group focused on the generation of human artificial thymic organoids (ATO) from umbilical cord blood derived hematopoietic stem cells to allow for the study of T cell development. (ca.gov)
Precursor1
  • However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
Gene expression4
  • This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)
  • The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
  • This analysis led to the discovery of a population of preadipocyte cells whose gene expression is remodeled in an age-specific fashion. (ca.gov)
  • For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
Interactions2
  • Cells can communicate via physical interactions, in- cluding membrane bridge formation, such as tunneling nanotubes and cytonemes, and/or through the release of soluble factors [1-3]. (unicyte.ch)
  • The fate of the cell is deter- mined by coordinated and dynamic interactions among a number of factors, acting in a defined microenviron- ment. (unicyte.ch)
Adult3
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • This finding provides insights into the mechanisms by which alterations in GSK-3 signaling affect adult human neurogenesis, a cellular process strongly suspected to play a role in the etiology of neuropsychiatric disorders. (gatech.edu)
  • 2001. Subchronic dermal application of N,N-diethyl m-toluamide (DEET) and permethrin to adult rats, alone or in combination, causes diffuse neuronal cell death and cytoskeletal abnormalities in the cerebral cortex and the hippocampus, and purkinje neuron loss in the cerebellum. (cdc.gov)
Tumors2
  • 2014). Human NK cells: From surface receptors to the therapy of leukemias and solid tumors. (springer.com)
  • Two students focused on establishing transgenic mouse models to explore the impact of androgen receptor, hepatocyte growth factor and human c-MET on the development of prostate cancer tumors. (ca.gov)
Suppress1
  • These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
Leukemias1
  • Engineered chimeric antigen receptor (CAR) T cell therapies (CAR-T) are now commercially available to treat certain leukemias and lymphomas. (bdbiosciences.com)
Endogenous2
  • Expression of versican 3'-untranslated region modulates endogenous microRNA functions. (sunnybrook.ca)
  • In this project, we want to mobilize endogenous stem and progenitor cells (SPCs) to improve tissue repair. (geigerlab.com)
Flow Cytometry1
  • We mostly used multicolor flow cytometry in these studies but we have also explored several applications for single-cell capillary electrophoresis (CE). (gatech.edu)
20211
  • 2021). Remdesivir induces persistent mitochondrial and structural damage in human induced pluripotent stem cell derived cardiomyocytes. (edu.hk)
Liver2
  • Interestingly, right after infection Kupffer cells disappear from the liver, whereas peripheral myeloid cells infiltrate the liver and later differentiated to cells that are very reminiscent to Kupffer cells. (twincore.de)
  • 2001. -Tocopherol modulates liver toxicity of the pyrethroid cypermethrin. (cdc.gov)
Mechanisms3
  • Understanding of molecular mechanisms in natural killer cell therapy. (springer.com)
  • Project A05 addresses the hypothesis that brain inflammaging is critically regulated by Notch/NF-kB crosstalk signaling which can promote synapse aging via cell-intrinsic and paracrine mechanisms finally resulting in motoric and cognitive decline. (uni-ulm.de)
  • In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
Additionally1
  • [ 2 ] Additionally, whole blood transfusion in civilian pre-hospital settings and in the trauma bay is seeing a resurgence in some regions. (medscape.com)
Transcription6
  • We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)
  • Within the field of Hematopoietic Stem Cells one trainee explored the impact of SGF29, a protein that recognizes methylated histones and modulates transcription, in leukemic cancer stem cells. (ca.gov)
  • Accordingly, a goal of microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. (justia.com)
  • Here we have determined the expression of a network of 18 key haematopoietic transcription factors in 597 single primary blood stem and progenitor cells isolated from mouse bone marrow. (ox.ac.uk)
  • We demonstrate that different stem/progenitor populations are characterized by distinctive transcription factor expression states, and through comprehensive bioinformatic analysis reveal positively and negatively correlated transcription factor pairings, including previously unrecognized relationships between Gata2, Gfi1 and Gfi1b. (ox.ac.uk)
  • Unlike sequence-specific transcription factors, epigenetic regulators do not necessarily bind DNA at consensus sequences, but still achieve reproducible target binding in a manner that is cell and maturation-type specific. (frontiersin.org)
Mesenchymal1
  • The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
Fate2
  • In these experiments, ATP-competitive (6-bromoindirubin-3'-oxime and CHIR99021) and substrate-competitive (TAT-eIF2B) inhibitors were used in order to eliminate potential confounding effects on cell fate due to off-target kinase inhibition. (gatech.edu)
  • In normal cells, the fate of pyruvate depends on many factors, one of which is oxygen availability. (hindawi.com)
Malignant2
  • In a study published in the Molecular Cell journal, they explain what makes hematopoietic stem cells acquire malignant traits in cancer. (lu.se)
  • Загальні відомості про лейкоз Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms. (msdmanuals.com)