MiRNAsSuppressesApoptosisGenesLong Non-codiSignaling pathwayEndogenousProgressionBiomarkersMoleculesPromotesMalignancyTranscriptionGene expressionPrognosisProliferation and invasionMRNAsEsophageal Squamous Cell CarcInvasionTissuesAcute MyeloidMRNANucleotidesMetastasisMiRNAGastricInhibitsAngiogenesisSquamousProteinEpithelial cellsRegulationSorafenibDifferentiationProteinsLncRNAOverexpressionTherapeutic targetMigrationDigestive system tumorsPTENCirrhosisEndothelialNeuronalTranscriptionalLiverPathwaysResistanceCancer cellCCND1BiologicalCellularExpression
MiRNAs26
- MicroRNAs (miRNAs) are small, endogenous, non-coding RNAs, which act as key post-transcriptional regulators of target-gene expression. (spandidos-publications.com)
- 1,000 human miRNAs have been identified that regulate ~1/3 of the coding genes in the human genome ( 9 ). (spandidos-publications.com)
- Many of these miRNAs act as tumor suppressors and/or oncogenes, and are involved in cell activities that include development, differentiation, proliferation, apoptosis, metabolism and immunity ( 7 , 10 , 11 ). (spandidos-publications.com)
- Another level of regulation may lie on microRNAs (miRNAs), which are endogenously expressed small non-coding RNAs of 18-24 nucleotides in length that regulate gene expression on the posttranscriptional level [ 14 ]. (biomedcentral.com)
- microRNAs also play a crucial role in endothelial inflammation, senescence and susceptibility to atherosclerosis: endothelial inflammation is critically regulated by miRNAs such as miR-126 and miR-10a, and endothelial aging is additionally controlled by miR-217 and miR-34a [ 15 ]. (biomedcentral.com)
- MicroRNAs (miRNAs) negatively regulate gene expression post-transcriptionally by binding to the 3'-untranslated region (3'-UTR) of the target mRNA sequences and have been shown to be involved in various types of cancers. (biomedcentral.com)
- MicroRNAs (miRNAs), with the length of 18-22 nucleotides, have been shown to downregulate target mRNA expression post-transcriptionally by binding to the 3'-untranslated region (UTR) of the target mRNA sequences [ 9 ]. (biomedcentral.com)
- microRNAs (miRNAs) have been emerged as a class of novel noncoding RNA regulator, and their length are approximately 22 nucleotides in length [ 4 ]. (hindawi.com)
- MicroRNAs (miRNAs) have become a hot topic in cancer research, but the underlying mechanism of its involvement in cancer remains unclear. (researchsquare.com)
- Recent studies, including our previous findings, have demonstrated that miRNAs play an important role in regulating biological functions [6-8]. (researchsquare.com)
- Because of their unique expression profile and special functions, miRNAs have been used as molecular markers to define the direction and processes of cell differentiation by regulating one or several target genes. (researchsquare.com)
- MiRNAs can also control the self-renewal or proliferation of stem cells [9]. (researchsquare.com)
- Previous studies have confirmed that miRNAs play an important role in gene regulation in CD44 + CD24 -/low breast cancer stem cells (BCSCs). (researchsquare.com)
- Emerging evidence indicates that Long non-coding RNAs (LncRNAs) and microRNAs (miRNAs) play crucial roles in tumor progression, including hepatocellular carcinoma (HCC). (biomedcentral.com)
- A series of studies indicate that lncRNAs serve as competing endogenous RNAs (ceRNA) by sponging miRNAs, and modulate the targets of miRNAs [ 15 , 16 ]. (biomedcentral.com)
- A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
- MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in the regulation of gene expression. (silexon.ai)
- MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of gene expression in many developmental and cellular processes. (biomedcentral.com)
- MicroRNAs (miRNAs) are an abundant class of small (approximately 22 nt) endogenous non-coding RNAs that direct post-transcriptional regulation of gene expression. (biomedcentral.com)
- miRNA genes are transcribed by RNA polymerase II into long primary miRNA transcripts, termed pri-miRNAs that are usually several kilobases long and possess a 5' CAP and a poly(A) tail. (biomedcentral.com)
- MiRNAs are characterized by binding the 3 - UTR of special mRNA to regulate the expression of the related proteins [ 10 , 11 ]. (hindawi.com)
- GSE94591, GSE116617, and GSE163386) were used to investigate dysregulated expressions of circular RNAs (circRNAs), and the GSE142699 and GSE142698 datasets were analyzed to detect dysregulated expressions of microRNAs (miRNAs) and mRNAs, respectively. (istanbul.edu.tr)
- DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)
- Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
- This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
- Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
Suppresses3
- A novel long non-coding RNA ENST00000480739 suppresses tumour cell invasion by regulating OS-9 and HIF-1α in pancreatic ductal adenocarcinoma. (aksomics.com)
- Inhibition of mu-opioid receptor suppresses proliferation of hepatocellular carcinoma cells via CD147-p53-MAPK cascade signaling pathway. (e-century.us)
- Circular RNA CREBBP suppresses hepatic fibrosis via targeting the hsa-miR-1291/LEFTY2 axis. (istanbul.edu.tr)
Apoptosis16
- EPCs from human umbilical cord and adult peripheral blood activate different mechanisms upon high-dose x-ray radiation treatment: CB-EPCs undergo p53 stabilization, Bax-dependent apoptosis and p21-dependent G 1 and G 2 /M cell cycle checkpoints, while PB-EPCs undergo only radiation-induced senescence [ 13 ], indicating unique gene expression patterns in EPCs of different sources. (biomedcentral.com)
- Then, agomir-20b-5p and antagomir-20b-5p were transfected into MCF-7 and T47D breast cancer cells to detect cell migration, wound healing and proliferation, and lentivirus vectors silencing or overexpressing miR-20b-5p were transfected into T47D-CSCs to detect proliferation and apoptosis. (researchsquare.com)
- Annexin V-PE/PI and PI staining were used to analyze cell apoptosis and cell cycle distribution by flow cytometry, respectively. (biomedcentral.com)
- Functional experiments demonstrated that knockdown of PTTG3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. (biomedcentral.com)
- SYVN1/GPX5 axis affects ischemia/reperfusion induced apoptosis of AC16 cells by regulating ROS generation. (e-century.us)
- Circular RNA circ_0084927 regulates proliferation, apoptosis, and invasion of breast cancer cells via miR-142-3p/ERC1 pathway. (e-century.us)
- Exosomes containing miR-122-5p secreted by LPS-induced neutrophils regulate the apoptosis and permeability of brain microvascular endothelial cells by targeting OCLN. (e-century.us)
- Molecular mechanisms and the influences of different regulation the expression of AKT1 on HCC cell growth, proliferation were determined by western blotting, MTT and colony formation assays, cell cycle and apoptosis were investigated by flow cytometry. (spandidos-publications.com)
- The phosphoinositide 3-kinase (PI3K)/RAC seine/threonine-protein kinase (AKT)/mechanistic target of rapamycin (mTOR) signaling pathway is a promising therapeutic target owing to its frequent dysregulation in HCC and the critical functions it has in regulating cell survival, proliferation, apoptosis, migration and angiogenesis through phosphorylation of distinct protein substrates ( 6 , 7 ). (spandidos-publications.com)
- However, the precise molecular mechanisms by which AKT1 promotes cell proliferation and regulates apoptosis ( 18 , 19 ) remain largely unclear. (spandidos-publications.com)
- High expression of activated AKT can be detected in HCC, and AKT may promote cell proliferation and regulation of cells apoptosis in HCC ( 20 , 21 ). (spandidos-publications.com)
- Subsequent mechanism investigations revealed that AKT1 served a notable function in cell proliferation and anti-apoptosis by directly regulating the expression of phosphatase and tensin homolog (PTEN) and Notch1. (spandidos-publications.com)
- Circular RNA circ_0040823 inhibits the proliferation of acute myeloid leukemia cells and induces apoptosis by regulating miR-516b/PTEN. (istanbul.edu.tr)
- Then, the chemical count kit-8 cell viability assay, Annexin V-FITC apoptosis detection, EdU labeling proliferation detection, transwell and wound healing experiments were performed. (researchsquare.com)
- The NFAT2 overexpression suppressed the cell viability, invasion and migration capabilities, and promoted apoptosis of HepG2 cells. (researchsquare.com)
- In this study, we constructed NFAT2 overexpressed cells (HepG2/NFAT2) based on HepG2 and investigated the effect of NFAT2 overexpression on HepG2 cells' viability, apoptosis, proliferation, invasion and migration. (researchsquare.com)
Genes13
- However, whether miR-497 regulates other target genes in HCC is unknown. (spandidos-publications.com)
- Recent studies demonstrated that miR-122 may directly regulate different aspects of the interferons (IFNs) signaling pathway to enhanced induction of anti-viral genes and inhibition of various virus. (wikipedia.org)
- EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. (biomedcentral.com)
- Stimulating the expression of angiogenic microRNAs or genes in EPCs of low activity (such as those from patients with cardiovascular diseases) might allow the development of novel therapeutic strategies. (biomedcentral.com)
- Dynamic Transcription of Long Non-Coding RNA Genes during CD4+ T Cell Development and Activation. (aksomics.com)
- Single-cell expression analysis with PACS was subsequently used to confirm a number of the differentially expressed genes identified with RNA sequencing. (rna-seqblog.com)
- We previously identified differentially expressed microRNAs with liver-specific target genes in plasma from children with chronic hepatitis B. To further understand the biological role of these microRNAs in the pathogenesis of chronic hepatitis B, we have used the human liver cell line HepG2, with and without HBV replication, after transfection of hepatitis B virus expression vectors. (biomedcentral.com)
- RT-qPCR is the preferred method for microRNA studies, and a careful normalisation strategy, verifying the optimal set of reference genes, is decisive for correctly evaluating microRNA expression levels. (biomedcentral.com)
- The aim of this study was to provide valid reference genes for the human HCC-derived cell line HepG2. (biomedcentral.com)
- A panel of 739 microRNAs was screened to identify the most stably expressed microRNAs, followed by a PubMed search identifying microRNAs previously used as reference genes. (biomedcentral.com)
- We identified miR-24-3p, miR-151a-5p, and miR-425-5p as the most valid combination of reference genes for microRNA RT-qPCR studies in our hepatitis B virus replicating HepG2 cell model. (biomedcentral.com)
- The present findings reveal that 3 IFN-β-regulated miRs and 37 genes, which are likely their functional targets, were commonly modulated by HCV in three replicon clones. (biomedcentral.com)
- Thus, the upstream genes that regulate PTEN may play an important role in the occurrence and development of tumors. (biomedcentral.com)
Long Non-codi7
- The aim of this mini-review is to discuss emerging epigenetic roles of long non-coding RNAs (lncRNAs) in Acute Myeloid Leukemia (AML). (frontiersin.org)
- In particular, there are incremental examples of the participation of the distinct class of long non-coding RNAs (lncRNAs) in AML epigenetic regulation, some of which have translational potential. (frontiersin.org)
- A distinct set of long non-coding RNAs in childhood MLL-rearranged acute lymphoblastic leukemia: biology and epigenetic target. (aksomics.com)
- Identifcation of differentially expressed long non-coding RNAs in CD4+ T cells response to latent tuberculosis infection. (aksomics.com)
- Expression Pattern of Long Non-coding RNAs in Renal Cell Carcinoma Revealed by Microarray. (aksomics.com)
- Microarray Expression Profile Analysis of Long Non-coding RNAs in Human Gastric Cardiac Adenocarcinoma. (aksomics.com)
- Pseudogene, a subclass of long non-coding RNAs (lncRNAs), are considered as genomic loci that resemble real gene, but lost some functionality because they are lack of protein-coding ability because of disabling mutation, lack of transcription, or their inability to encode RNA [ 3 ]. (biomedcentral.com)
Signaling pathway6
- Finally, IGF‑1R protein, phosphoinositide 3‑kinase/Akt signaling pathway‑associated proteins and cyclin pathway‑associated proteins were differentially expressed between miR‑497‑overexpressing cells and miR-497-silenced cells. (spandidos-publications.com)
- In this review, we discuss about the significance of glioblastoma stem cells in cancer progression through Wnt signaling pathway and highlight the clinical targets being potentially considered for therapy in glioblastoma. (biomedcentral.com)
- Wnt signaling pathway is mediated by Wnt family of 19 secreted glycoproteins (bearing22 or 24 cysteine residues) which are essential for cell proliferation, embryonic development, cell polarity, and tissue homeostasis [ 9 ]. (biomedcentral.com)
- Canonical Wnt signaling pathway is essential for embryonic development [ 10 ] and non-canonical Wnt pathway regulates cell movement and tissue polarity [ 11 ]. (biomedcentral.com)
- The current review focuses on the importance of Wnt signaling pathway in glioblastoma stem cell progression and explores targeting of Wnt signaling molecules as a potential therapy for glioblastoma. (biomedcentral.com)
- AKT is a key molecule in the PI3K/AKT/mTOR signaling pathway, which has been shown to serve notable functions in the regulation of cell viability and to be closely associated with a variety of disorders caused by dysfunctional cellular proliferation ( 8 , 9 ). (spandidos-publications.com)
Endogenous3
- A long noncoding RNA critically regulates Bcr-Abl-mediated cellular transformation by acting as a competitive endogenous RNA. (aksomics.com)
- Mechanistically, PTTG3P upregulated the expression of miR-383 targets Cyclin D1 (CCND1) and poly ADP-ribose polymerase 2 (PARP2) by sponging miR-383, acting as a competing endogenous RNA (ceRNA). (biomedcentral.com)
- Identification of circRNA-lncRNA-miRNA-mRNA competitive endogenous rna network as novel prognostic markers for acute myeloid leukemia. (istanbul.edu.tr)
Progression11
- Additionally, miR-146a silencing or restoration of FLAP led to a reduction of HepG2 cell proliferation, cell cycle progression, migration, and invasion. (biomedcentral.com)
- Although microRNA-29a-3p was reported to inhibit laryngocarcinoma progression, the potential mechanisms have not been explored clearly. (hindawi.com)
- MiR-29a-3p was enriched in hBMSC-derived Exo, and the Exo from miR-29a-3p mimics transfected hBMSCs could inhibit laryngocarcinoma cell malignant phenotypes in vitro and prevent tumor progression in vivo . (hindawi.com)
- In conclusion, hBMSC-derived Exo with upregulated miR-29a-3p inhibited laryngocarcinoma progression via regulating PTEN, providing a potential diagnostic and therapeutic target in this disease. (hindawi.com)
- Previous studies revealed that inhibition of miR-744-3p could restore PTEN expression, and then suppressed laryngeal squamous cell carcinoma progression [ 20 ], suggesting that upregulation of PTEN could contribute to cancer development. (hindawi.com)
- LncRNA AGPG has been shown to play a role in esophageal cancer by binding to and stabilizing PFKB3, which increases glycolytic flux and cell cycle progression in esophageal cancer cells. (ijbs.com)
- Knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells, and the biological effect of Annexin A2 on proliferation and invasion was mediated by the inhibition of p53, which in turn, promoted cell cycle progression and EMT. (jcancer.org)
- Consequently, the molecular mechanisms that regulate GC development and progression need further exploration. (oncotarget.com)
- Identifying small molecules or oligonucleotides that can modulate MIR374B activity may help restore its normal expression levels and combat disease progression. (silexon.ai)
- For example, in hepatocellular carcinoma, the downregulation of MIR374B has been associated with advanced disease stages and poor prognosis, making it a potential biomarker for disease progression. (silexon.ai)
- It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. (thermofisher.com)
Biomarkers5
- Long Non-Coding RNA Profiling in Laryngeal Squamous Cell Carcinoma and Its Clinical Significance: Potential Biomarkers for LSCC. (aksomics.com)
- MicroRNAs are regulatory molecules and suggested as non-invasive biomarkers for molecular diagnostics and prognostics. (biomedcentral.com)
- MicroRNAs are suggested as non-invasive biomarkers for molecular diagnostics and prognostics, and some microRNAs show promising results as therapeutic targets in human trials [ 1 - 3 ]. (biomedcentral.com)
- Circulating microRNAs from serum exosomes as potential biomarkers in patients with spontaneous abortion. (e-century.us)
- Therefore, research on hepatocellular transformations in initiation and development of HCC and identifying effective biomarkers for novel therapeutic option are still emergency challenges. (researchsquare.com)
Molecules8
- miR-122 regulates the expression of several mRNA molecules that are important in the circadian cycle, such as PPARβ/δ. (wikipedia.org)
- A growing body of evidence indicates that epigenetic regulators rely, amongst other factors, on their interaction with untranslated RNA molecules for guidance to particular targets on DNA. (frontiersin.org)
- Non (protein)-coding RNAs are the most abundant transcriptional products of the coding genome, and comprise several different classes of molecules with unique lengths, conformations and targets. (frontiersin.org)
- NcRNAs are a class of RNA molecules that do not encode proteins, and they play an important role in regulating cellular transcription and post-transcriptional processes [ 7 ]. (ijbs.com)
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- They function by binding to messenger RNA (mRNA) molecules and inhibiting their translation into protein. (silexon.ai)
- ASOs are short synthetic DNA or RNA molecules that can bind to specific mRNA or miRNA targets, preventing their function or promoting their degradation. (silexon.ai)
- Small molecules can interfere with miRNA biogenesis or activity, offering a potential means to modulate MIR374B expression. (silexon.ai)
Promotes10
- This study showed that miR-146a has a stimulatory role in HepG2 cells and promotes HepG2 cell migration and invasion by targeting FLAP mRNA. (biomedcentral.com)
- For example, miR-552 promotes cell proliferative rate of laryngocarcinoma cells by modulating p53 signaling [ 5 ]. (hindawi.com)
- However, another article reported that ALKBH5 promotes hepatocellular carcinogenesis in HBV-related HCC and catalyzes m6A demethylation of HBx mRNA to sustain its expression [ 23 ]. (biomedcentral.com)
- Long Noncoding RNA Activated by TGF-β Promotes the Invasion-Metastasis Cascade in Hepatocellular Carcinoma. (aksomics.com)
- Long non-coding RNA MRUL promotes ABCB1 expression in multidrug-resistant gastric cancer cell sublines. (aksomics.com)
- Wnt deregulation in brain is associated with congenital disorders [ 7 ], whereas it promotes malignancy in somatic cells of neuronal origin [ 8 ]. (biomedcentral.com)
- KIF22 promotes progress of esophageal squamous cell carcinoma cells and is negatively regulated by miR-122. (e-century.us)
- CASC11 promotes aggressiveness of prostate cancer cells through miR-145/IGF1R axis. (istanbul.edu.tr)
- CircRNA_104889 promotes lung adenocarcinoma cell invasion via sponging miR4458. (istanbul.edu.tr)
- 17. Jiang YM, Liu W, Jiang L, Chang H. CircLDLR promotes papillary thyroid carcinoma tumorigenicity by regulating miR-637/LMO4 Axis. (istanbul.edu.tr)
Malignancy4
- Small cell lung cancer (SCLC) is an exceptionally lethal malignancy for which more effective therapies are urgently needed. (cancerindex.org)
- Nuclear factor of activated T cells 2 (NFAT2) has been reported to regulate the development and malignancy of few tumors. (researchsquare.com)
- The current study firstly suggested that NFAT2 suppressed the aggression and malignancy of HepG2 cells through inducing the expression of Egr2. (researchsquare.com)
- Therefore, besides the regulation of immune cells, NFAT2 also exerts modulatory effect of proliferation, invasion, metastasis and malignancy in breast cancer, lung cancer, melanoma and leukemia [13-15]. (researchsquare.com)
Transcription7
- Therefore, the present study investigated miR‑497 expression in HCC samples and HCC‑derived cell lines using reverse transcription‑quantitative polymerase chain reaction. (spandidos-publications.com)
- Transcription is regulated by HNF4α. (wikipedia.org)
- Unlike sequence-specific transcription factors, epigenetic regulators do not necessarily bind DNA at consensus sequences, but still achieve reproducible target binding in a manner that is cell and maturation-type specific. (frontiersin.org)
- NRAV, a Long Noncoding RNA, Modulates Antiviral Responses through Suppression of Interferon-Stimulated Gene Transcription. (aksomics.com)
- It has shown that miR-429 could inhibit the development of colorectal cancer via targeting in large tumor suppressor kinase (LATS2), and decreased miR-429 could promote the expression of SRY-box transcription factor 2 (SOX2) and B-cell lymphoma 2 (BCL2) to attenuate the neuronal injury induced by accumulation of amyloid β -protein [ 12 ]. (hindawi.com)
- Western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) to evaluate the expression levels of AKT1 in HCC SMMC‑7721 cell line. (spandidos-publications.com)
- In a PCR array analysis of 84 transcription factors, either overexpressing DEFB1 or siRNA silencing of DEFB1 expression significantly modulated the expression of signal transducer and activator of transcription 3 (STAT3). (cdc.gov)
Gene expression4
- There is evidence that miR-122 is regulated by Rev-ErbA alpha which is involved in circadian gene expression, suggesting that miR-122 is a circadian metabolic regulator. (wikipedia.org)
- NcRNAs play a significant role in regulating gene expression in digestive system tumors. (ijbs.com)
- Gene expression-based prognostic signatures have a significant effect on predicting the survival of patients with malignant tumors, such as non-small cell lung cancer [ 7 ] and pediatric acute myeloid leukemia [ 8 ]. (aging-us.com)
- MicroRNAs are endogenously expressed, small non-coding RNAs that regulate gene expression by targeting mRNAs for translational repression or degradation. (oncotarget.com)
Prognosis3
- miR-122 levels are frequently reduced in hepatocellular carcinoma (HCC) compared to normal liver, and low miR-122 levels correlate with poor prognosis. (wikipedia.org)
- Long noncoding RNA GAPLINC regulates CD44-dependent cell invasiveness and associates with poor prognosis of gastric cancer. (aksomics.com)
- Primary liver cancer, which consists mainly of hepatocellular carcinoma (HCC), is the third-leading cause of cancer mortality worldwide, following lung and stomach cancer, owing to its poor prognosis and frequent relapse and metastasis ( 1 , 2 ). (spandidos-publications.com)
Proliferation and invasion1
- Moreover, we found that knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells. (jcancer.org)
MRNAs1
- miR-122 also regulates systemic iron homeostasis via the target mRNAs Hjv and Hfe. (wikipedia.org)
Esophageal Squamous Cell Carc1
- In a whole-genome sequencing in 17 Esophageal Squamous Cell Carcinoma cases and whole-exome sequencing in 71 cases, Song (2014), reported ASH1L alterations. (cancerindex.org)
Invasion4
- The miR mimics or inhibitor was transfected into laryngocarcinoma cell lines M4E and Hep2 for the investigation of the biological functions (proliferative, invasion, migratory rates, and apoptotic rates) of this miRNA. (hindawi.com)
- Transwell migration and invasion assays were used to examine cell migration and invasion abilities. (biomedcentral.com)
- Annexin A2 has been involved in cancer cell adhesion, invasion and metastasis. (jcancer.org)
- Furthermore, NFAT2 participates in the early growth response factor 2 (Egr2)-regulated anergic phenotype which impairs the migration and invasion of leukaemia cells [16]. (researchsquare.com)
Tissues8
- Herein, we aimed to demonstrate the expression of miR-146a and FLAP in human HCC tissues and liver cancer cell lines. (biomedcentral.com)
- We demonstrated that miR-146a expression is overexpressed, while FLAP protein and mRNA are suppressed in hepatocellular carcinoma tissues and HepG2 cells compared to para-carcinoma tissues and HL-7702 cells. (biomedcentral.com)
- The miR-29a-3p level was decreased, and PTEN level was elevated in laryngocarcinoma tissues and the cancer cell lines. (hindawi.com)
- In this study, we found that PTTG3P was up-regulated in HCC tissues and cells. (biomedcentral.com)
- In this study, we showed that Annexin A2 was evidently overexpressed in human NSCLCs cell lines and NSCLCs tissues. (jcancer.org)
- At last, the mRNA and protein expression of NFAT2, Egr2, FasL, COX-2 and c-myc in carcinoma and adjacent tissues was investigated. (researchsquare.com)
- Compared with adjacent tissues, the carcinoma tissues expressed less NFAT2, Egr2, FasL and more COX-2 and c-myc. (researchsquare.com)
- The absence of NFAT2 and Egr2 in carcinoma tissues reminded us that NFAT2 may be a promising therapeutic target for hepatocellular carcinoma treatment. (researchsquare.com)
Acute Myeloid2
- Objective: Acute myeloid leukemia (AML) is a lethal type of cancer associated with dysregulation of progenitor hematopoietic stem cell behavior and its incidence is, unfortunately, increasing. (istanbul.edu.tr)
- Role of circular RNA DLEU2 in human acute myeloid leukemia. (istanbul.edu.tr)
MRNA5
- This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. (nih.gov)
- miR-122 regulates the synthesis of the protein CAT-1 by binding to sites in the mRNA 3'UTR such that translation is repressed and the mRNA is targeted to P bodies. (wikipedia.org)
- The Long Intergenic Noncoding RNA UFC1, a Target of microRNA 34a, Interacts with the mRNA Stabilizing Protein HuR to Increase Levels of β-catenin in HCC Cells. (aksomics.com)
- The Role of PinX1 in Growth Control of Breast Cancer Cells and Its Potential Molecular Mechanism by mRNA and lncRNA Expression Profiles Screening. (aksomics.com)
- The researchers have now validated a method for performing next-generation sequencing on mRNA obtained from PACS isolated cells. (rna-seqblog.com)
Nucleotides1
- MicroRNAs (miRNA), as noncoding single RNA with the length of approximate 20 nucleotides, have been confirmed as key factors in multiple activities of cells [ 6 , 7 ]. (hindawi.com)
Metastasis1
- Its downregulation is often observed in these cancers, contributing to uncontrolled cell proliferation and metastasis. (silexon.ai)
MiRNA10
- The Argonaute proteins (Ago1-4), which are essential for miRNA-directed repression, appear to be necessary for miR-122 to regulate HCV, although miR-122 overexpression may overcome this requirement. (wikipedia.org)
- The miRNA profile between breast cancer stem cells(BCSCs, CD44+CD24-/low) and control MCF-7 breast cancer cells was obtained in a previous study. (researchsquare.com)
- MiR-20b-5p had the highest degree in both the miRNA-gene network and miRNA-GO network to regulate BCSCs. (researchsquare.com)
- In our previous research, we performed miRNA profiling between sorted CD44 + CD24 -/low BCSCs and the control MCF-7 breast cancer cells [8]. (researchsquare.com)
- Similarly, the miRNA signature shows predictive value in adults with T-cell lymphoblastic lymphoma [ 9 ], and long noncoding RNA shows predictive value in adults with localized clear cell renal cell carcinoma [ 10 ]. (aging-us.com)
- The recent explosion in miRNA research has accelerated the development of several computational and experimental approaches for probing miRNA functions in cell culture and in vivo . (biomedcentral.com)
- MicroRNA (miRNA) dysfunction has been confirmed as a key event of ischemic stroke appearance. (hindawi.com)
- This study is a continuation of our earlier findings involving poly(ε-caprolactone) (PCL)-poly (ethylene glycol) (PEG) nanoparticles (PEG-PCL NPs), which were coated with trastuzumab to target gastric cancer cells with HER2 receptor over-expression using anti-miRNA-21 antisense oligonucleotides (AMO-21). (oncotarget.com)
- Based on our identified miRNA-regulated network, we discovered three drugsBX-912, daunorubicin, and midostaurinthat can be repositioned to treat lung cancer, which was not known before. (cdc.gov)
- Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)
Gastric4
- Recently, an increasing number of studies have revealed that microRNA-497 (miR-497) levels are decreased in tumors, and that it functions as a tumor suppressor in a number of types of human cancer, including colorectal, gastric, cervical and breast cancers, adrenocortical carcinoma and melanoma ( 12 - 20 ). (spandidos-publications.com)
- Studies have indicated that MIR374B acts as a tumor suppressor in several cancer types, including gastric cancer, hepatocellular carcinoma, and breast cancer. (silexon.ai)
- Global circular RNA expression profile of human gastric cancer and its clinical significance. (istanbul.edu.tr)
- Overexpression of miR-21 decreases the sensitivity of gastric cancer cells to trastuzumab, which is a humanized monoclonal antibody targeting human epidermal growth factor receptor 2. (oncotarget.com)
Inhibits2
- Transmembrane protein 88 inhibits transforming growth factor-β1-induced-extracellular matrix accumulation and epithelial-mesenchymal transition program in human pleural mesothelial cells through modulating TGF-β1/Smad pathway. (nih.gov)
- We revealed the mechanism by which NCAPG regulates the proliferation of HCC: NCAPG inhibits PTEN through its interaction with CKII, and then activates the PI3K-AKT pathway to promote the proliferation of HCC. (biomedcentral.com)
Angiogenesis4
- microRNAs have emerged as master regulators of stem cell lineage differentiation and angiogenesis [ 14 ]. (biomedcentral.com)
- Here, gain- and loss-of-function assays demonstrated that miR-874 inhibited the tumor angiogenesis of GC cells in vitro and in vivo . (oncotarget.com)
- SNAI2, an angiogenesis-related factor, was highly expressed under the high glucose condition and also led to viability and migration of human brain microvascular endothelial cells (HBMECs) [ 13 ]. (hindawi.com)
- Highly enriched exosomal lncRNA OIP5-AS1 regulates osteosarcoma tumor angiogenesis and autophagy through miR-153 and ATG5. (e-century.us)
Squamous1
- NSCLCs including adenocarcinoma, squamous cell carcinoma and large cell carcinoma, constitute 80% of all lung cancers [ 2 ]. (jcancer.org)
Protein7
- In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. (nih.gov)
- This repression can be relieved by the protein HuR, which is released from the nucleus under conditions of cell stress and binds to the CAT-1 3'UTR. (wikipedia.org)
- However, a growing body of evidence suggests that recruitment, stability, and function of epigenetic factors can be mediated by non-protein-coding RNAs. (frontiersin.org)
- Abnormal expression of 5-Lipoxygenase Activating Protein (FLAP) has been detected in many tumor cells. (biomedcentral.com)
- Tonicity-responsive enhancer-binding protein (TonEBP), which is also known as nuclear factor of activated T cells 5 (NFAT5), was discovered 20 years ago as a transcriptional regulator of the cellular response to hypertonic (hyperosmotic salinity) stress in the renal medulla. (nature.com)
- The RAC serine/threonine-protein kinase (AKT) family of serine/threonine protein kinases, particularly the AKT1 isoform, has been identified abnormally expressed in hepatocellular carcinoma (HCC) cells, and is highly associated with cell behavior, including proliferation, survival, metabolism, and tumorigenesis. (spandidos-publications.com)
- NFAT2, as an important transcriptional promoter, regulates expression of TNF-α, myc proto-oncogene protein (c-myc), cyclooxygenase-2 (COX-2), Fas ligand (FasL) and also generates crosstalks with ERK/MAPK pathway and AKT/GSK3b signaling, which achieves its control of the cell fate [7-12]. (researchsquare.com)
Epithelial cells2
- The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
- Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
Regulation4
- HCV RNA synthesis is not affected by miR-122, suggesting that regulation of other processes such as RNA stability may occur. (wikipedia.org)
- Dynamic regulation of RNA methylation mainly depends on ''writers'' and ''erasers'', which function as dedicated RNA methyltransferases and demethylases, respectively [ 10 ]. (biomedcentral.com)
- Even small changes in microRNA expression may therefore have implications for gene regulation in various physiological and pathophysiological states [ 1 , 6 ]. (biomedcentral.com)
- Furthermore, the data of the present study indicated that B‑cell lymphoma 2 and cyclin D1 is involved in the regulation of AKT1 expression. (spandidos-publications.com)
Sorafenib2
- Overexpression of miR-122 reduces tumorigenic properties in HCC cell lines, suggesting that it functions as a tumor suppressor gene, and increases the response of cells to the chemotherapeutic drugs sorafenib and doxorubicin. (wikipedia.org)
- The mainstay of treatment for advanced hepatocellular carcinoma (HCC) was the tyrosine kinase inhibitor (TKI) sorafenib [ 5 ]. (biomedcentral.com)
Differentiation2
- Akin to the normal hematopoietic system, leukemias are sustained by a small number of leukemia stem-like cells (LSC), which can be distinct from the normal hematopoietic stem cells (HSC) but also exhibit functional characteristics of self-renewal and (abnormal or hindered) differentiation, and are often quiescent ( 2 - 4 ). (frontiersin.org)
- Nuclear factor of activated T cells 2 (NFAT2, also known as NFATc and NFATc1) is an important member of NFAT family which plays a vital role in T cell activation and differentiation [4]. (researchsquare.com)
Proteins1
- Thus, we compared the expression profile of 24 selected miRs in IFN-β-treated Huh-7 cells and in three different clones of Huh-7 cells carrying a self-replicating HCV RNA which express all viral proteins (HCV replicon system). (biomedcentral.com)
LncRNA3
- MiR-125b participates in the oncogenic role of lncRNA MALAT1 in the phenotypes including cell proliferative and invasive rates in laryngocarcinoma [ 6 ]. (hindawi.com)
- LncRNA-regulated infection and inflammation pathways associated with pregnancy loss: Genome wide differential expression of lncRNAs in early spontaneous abortion. (aksomics.com)
- Luciferase reporter assay and RNA pull-down assay were carried out to detect the interaction between miR-383 and LncRNA PTTG3P. (biomedcentral.com)
Overexpression4
- In addition, overexpression of miR‑497 was observed to inhibit colony formation and tumor growth in MHCC‑97H human HCC cells. (spandidos-publications.com)
- The effect of miR-20b-5p on xenograft growth was investigated in vivo by transfection of a lentivirus-overexpression vector into T47D cells. (researchsquare.com)
- It has been reported that overexpression of AKT is associated with decreased disease-free survival rates and development of primary carcinomas of the prostate, breast and ovary ( 12 , 13 ). (spandidos-publications.com)
- The sensitivity and Ca 2+ mobilization of HepG2 cells was also inhibited by NFAT2 overexpression. (researchsquare.com)
Therapeutic target1
- For instance, MIR374B has been found to be down-regulated in patients with heart failure, suggesting its potential as a therapeutic target in cardiac diseases. (silexon.ai)
Migration1
- Knocking down miR-31 levels in CB-EPCs suppressed cell migration and microtubule formation, while overexpressing miR-31 in PB-EPCs helped to recapitulate some of CB-EPC functions. (biomedcentral.com)
Digestive system tumors1
- Hepatocellular carcinoma (HCC) one of the most common digestive system tumors, threatens the tens of thousands of people with high morbidity and mortality world widely. (biomedcentral.com)
PTEN1
- The exosomes (Exo) from human bone marrow mesenchymal stem cells (hBMSCs) after the transfection of miR mimics/inhibitor/si-PTEN were isolated and used to stimulate M4E and Hep2 cells. (hindawi.com)
Cirrhosis2
- NAFLD can progress to non-alcoholic steatohepatitis (NASH) and increases the risk for cirrhosis and hepatic carcinoma. (biomedcentral.com)
- After a sub-clinical phase, greater than 80% of patients progress to persistent HCV infection, the leading cause of chronic liver disease associated with cirrhosis and hepatocellular carcinoma [ 1 , 2 ]. (biomedcentral.com)
Endothelial4
- Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair, yet EPCs from different anatomic locations possess unique biological properties. (biomedcentral.com)
- Blood-derived endothelial progenitor cells (EPCs) represent the "promoters" of vascular repair providing the rationale for autologous stem cell therapy [ 2 ]. (biomedcentral.com)
- MiR-146a has also been shown to promote the angiogenic activity of endothelial cells in HCC patients [ 11 ]. (biomedcentral.com)
- Annexin A2 (also called ANXA2) is mainly expressed in endothelial cells. (jcancer.org)
Neuronal1
- Moreover, researchers have discovered that MIR374B expression is altered in neurodegenerative disorders like Alzheimer鈥檚 disease, highlighting its role in regulating neuronal health. (silexon.ai)
Transcriptional1
- This capability makes PACS well suited for transcriptional profiling of rare cells from complex populations to obtain maximal biological insight into cell states and behaviors. (rna-seqblog.com)
Liver3
- Hepatocellular carcinoma (HCC) is the most common primary cancer of the liver. (spandidos-publications.com)
- Instead we use an in vitro HBV-replicating liver cell model to investigate the biological role of microRNAs with a possible influence on the pathogenesis of CHB in children. (biomedcentral.com)
- Stem cell transplantation for treating liver diseases: progress and remaining challenges. (e-century.us)
Pathways4
- Glioblastoma stem cells play an important role in tumor formation by activation of several signaling pathways. (biomedcentral.com)
- There are two Wnt pathways which determine the cell fate. (biomedcentral.com)
- A single microRNA can have multiple downstream targets and affect a number of different networks and pathways [ 4 , 5 ]. (biomedcentral.com)
- In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
Resistance4
- GSCs are implicated for recurrence due to resistance to therapy, thereby allowing cell renewal and tumor re-initiation, while physically, GSCs are difficult to target because of their presence in the perivascular space [ 24 ]. (biomedcentral.com)
- In addition, functional studies have shown that TonEBP is involved in the pathogenesis of rheumatoid arthritis, atherosclerosis, diabetic nephropathy, acute kidney injury, hyperlipidaemia and insulin resistance, autoimmune diseases (including type 1 diabetes mellitus and multiple sclerosis), salt-sensitive hypertension and hepatocellular carcinoma. (nature.com)
- Cancer cells frequently display intrinsic or acquired resistance to chemically diverse anticancer drugs, limiting therapeutic success. (nature.com)
- Biedler, J. L. & Riehm, H. Cellular resistance to actinomycin D in Chinese hamster cells in vitro: cross-resistance, radioautographic, and cytogenetic studies. (nature.com)
Cancer cell1
- Cancer Cell. (aksomics.com)
CCND11
- The PTTG3P-miR-383-CCND1/PARP2 axis modulated HCC phenotypes. (biomedcentral.com)
Biological1
- MicroRNAs, including MIR374B, are stable in various biological fluids, such as blood and urine, and their expression levels can be quantified using highly sensitive techniques like quantitative polymerase chain reaction (qPCR) or next-generation sequencing. (silexon.ai)
Cellular3
- One such molecule that has garnered attention is MIR374B, a microRNA that plays a crucial role in cellular processes and has the potential to be a promising target for therapeutic interventions. (silexon.ai)
- MIR374B, also known as hsa-miR-374b-5p, is a specific microRNA involved in various cellular processes. (silexon.ai)
- The cell line specificity, cellular uptake, AMO-21 delivery, and cytotoxicity of the HER-PEG-PCL NPs were investigated. (oncotarget.com)
Expression13
- HepG2 cells with overexpressed microRNA-122 mount an effective antiviral interferon response and innate immune response to hepatitis C virus (HCV), other RNA viruses and viral mimetics (e.g. poly(I:C)). Recent studies have shown that replication of hepatitis C virus (HCV) is dependent on miR-122 expression. (wikipedia.org)
- Knockdown of miR-146a also resulted in increased FLAP expression of cancer cells. (biomedcentral.com)
- Expression profile of long noncoding RNAs in cartilage from knee osteoarthritis patients. (aksomics.com)
- Analysis of Altered Microarray Expression Profiles in Proximal Renal Tubular Cells in Response to Calcium Oxalate Monohydrate Crystal Adhesion: Implications for Kidney Stone Disease. (aksomics.com)
- The Long Noncoding RNA Expression Profile of Hepatocellular Carcinoma Identified by Microarray Analysis. (aksomics.com)
- Mechanistically, our studies showed that knockdown of Annexin A2 increased the expression of p53, which in turn, induced cell cycle G2 arrest and inhibited epithelial-to-mesenchymal transition (EMT). (jcancer.org)
- Altered expression levels of specific microRNAs are associated with hepatitis B virus infection and hepatocellular carcinoma. (biomedcentral.com)
- The present study was conducted to identify suitable RGs for microRNA expression studies in a human HCC-derived cell line (HepG2 tet-on), with and without HBV replication, after transfection of HBV expression vectors. (biomedcentral.com)
- The qRT-PCR was used to measure the expression levels of the miR-429 in the serums of the patients or cells, and CCK-8, wound healing assay, and tube formation assay were used to observe the effects of miR-429 on the phenotype of HBMECs. (hindawi.com)
- To date, the effect produced by HCV on the expression of microRNAs (miRs) involved in the interferon-β (IFN-β) antiviral pathway has not been explored in details. (biomedcentral.com)
- The expression profile of 24 selected miRs in IFN-β-treated Huh-7 cells and in HCV replicon 21-5 clone with respect to Huh-7 parental cells was analysed by real-time PCR. (biomedcentral.com)
- The expression profile revealed that 16 out of 24 miRs were modulated in HCV replicon clone 21-5. (biomedcentral.com)
- In this study, we aimed to explore the effect of NFAT2 expression on cell fate of HepG2 cell and its potential mechanisms. (researchsquare.com)