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Chromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, Ion ExchangeChromatography, GasChromatography, Thin LayerChromatography, LiquidChromatography, DEAE-CelluloseMolecular WeightElectrophoresis, Polyacrylamide GelChromatography, AgaroseMass SpectrometryGas Chromatography-Mass SpectrometryChromatography, Reverse-PhaseMolecular Sequence DataAmino Acid SequenceKineticsChromatography, PaperAmino AcidsHydrogen-Ion ConcentrationSepharoseSubstrate SpecificitySpectrophotometry, UltravioletChemistryChemical PhenomenaTandem Mass SpectrometryCattleSpectrometry, Mass, Electrospray IonizationCarbohydratesIsoelectric FocusingCountercurrent DistributionChromatography, Micellar Electrokinetic CapillaryChemical FractionationCarbohydrate SequenceEscherichia coliMethodsIsoelectric PointOligosaccharidesPeptide FragmentsDrug StabilityMagnetic Resonance SpectroscopyRabbitsLiverHydrolysisTrypsinProtein BindingRecombinant ProteinsMacromolecular SubstancesSolubilityTemperatureIndicators and ReagentsImmunodiffusionHydroxyapatitesPeptidesReference StandardsCalibrationGlycoproteinsMicrochemistryBase SequenceReproducibility of ResultsPolysaccharidesRadioimmunoassayCloning, MolecularFatty AcidsAcetonitrilesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationCarbohydrate ConformationBinding SitesStereoisomerismBiological AssayMolecular StructureAmmonium SulfateChemical PrecipitationLectinsSwineIsoenzymesLimit of DetectionSolid Phase ExtractionGlycoside HydrolasesCentrifugation, Density GradientHot TemperatureAnion Exchange ResinsPlant ExtractsBrain ChemistryChemistry Techniques, AnalyticalSpecies SpecificityMethanolSolventsBacterial ProteinsCell LineGlycopeptidesRats, Inbred StrainsFlame IonizationPeptide MappingSequence Homology, Amino AcidCarrier ProteinsUltracentrifugationSpectrometry, Mass, Fast Atom BombardmentPhospholipids
Size exclusion chroLiquid chromatographySeparationDispersionSpectroscopyBehaviorProbabilisticStructuralMembrane2020FlowsCharacterization2019DetectionAnalyzeCharacteristicsVariabilityApproachesQuantitative modelsResultsStructuresNumericalPerformanceTimeRoleEasilyLowerColumnUnitUniqueSystemsTechniquePotentialHighSolidCancerCaseSolutionsProcessStudyShownStatic
Size exclusion chro4
  • After large-scale expression in bacteria, ultra-high purity TF-MCP fusion protein was obtained by affinity chromatography and size exclusion chromatography. (utep.edu)
  • The conventional characterization technique, size exclusion chromatography (SEC), is the proper choice as long as the proteins are small and stable. (pegsummiteurope.com)
  • Viscotek's triple detection SEC/GPC can provide good size exclusion chromatography as well as advanced detection capabilities to correlate molecular weight and size properties to critical performance parameters. (malvernpanalytical.com)
  • 695). The size exclusion chromatography (SEC) of the synthesized diblock copolymers xvii reveals the presence of some dead chains in P(POSS-MA-co-S)x macroinitiator. (gov.pk)
Liquid chromatography7
  • Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid-liquid chromatography that uses a liquid stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force and is used to separate, identify, and quantify the chemical components of a mixture. (wikipedia.org)
  • In its broadest sense, countercurrent chromatography encompasses a collection of related liquid chromatography techniques that employ two immiscible liquid phases without a solid support. (wikipedia.org)
  • The components of a CCC system are similar to most liquid chromatography configurations, such as high-performance liquid chromatography (HPLC). (wikipedia.org)
  • By contrast, in liquid-liquid chromatography, both the mobile and stationary phases are liquid. (wikipedia.org)
  • 2019). Development and Validation of a Simple and Efficient Method for the Determination of Pendimethalin and its Metabolite M455H001 in Soil by Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS), Analytical Letters , Vol. 52, No. 4, pp. 685-696 ( http://dx.doi.org/10.1080/00032719.2018.1486849 ). (ntua.gr)
  • For the first time in New Zealand, an analytical method using reversed-phase ion-pair liquid chromatography, was established to determine EDTA present in dairy wastewater, and then applied to surface water, soils and groundwater with appropriate modifications. (waikato.ac.nz)
  • The current study describes the implementation of a hybridization liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform for the direct quantitation of antisense oligonucleotide (ASO) ƒ u . (aspetjournals.org)
Separation3
  • With column chromatography, the separation potential is limited by the commercially available stationary phase media and its particular characteristics. (wikipedia.org)
  • Thermodynamic, hydrodynamic, scattering and separation methods for investigation and characterization of polymer systems are described. (vscht.cz)
  • Separation was performed on two ViscoGEL C-series columns while maintaining a constant temperature of 30 °C during chromatography and detection. (malvernpanalytical.com)
Dispersion6
  • This thesis is dedicated to the study of mass transport processes (flow, diffusion, and hydrodynamic dispersion) in computer-generated random sphere packings. (uni-marburg.de)
  • The first part of this thesis investigates the influence of the cross-sectional geometry of the confined random sphere packings on the hydrodynamic dispersion. (uni-marburg.de)
  • Packings with different values of porosity (interstitial void space fraction) generated in containers of circular, quadratic, rectangular, trapezoidal, and irregular (reconstructed) geometries were studied, and resulting pre-asymptotic and close-to-asymptotic hydrodynamic dispersion coefficients were analyzed. (uni-marburg.de)
  • It was demonstrated i) a significant impact of the cross-sectional geometry and porosity on the hydrodynamic dispersion coefficients, and ii) reduction of the symmetry of the cross section results in longer times to reach close-to-asymptotic values and larger absolute values of the hydrodynamic dispersion coefficients. (uni-marburg.de)
  • ii) influence of the packing microstructural disorder on the effective diffusion and hydrodynamic dispersion coefficients was investigated and clear qualitative corellation with geometrical descriptors (which are based on Delaunay and Voronoi spatial tessellations) was demonstrated. (uni-marburg.de)
  • A quasi one-dimension vertical mixing model and a two-dimension (depth-averaged) 3DD hydrodynamic model were applied to simulate EDTA dispersion in the river. (waikato.ac.nz)
Spectroscopy2
  • Methods used include use of spectroscopy, chromatography, polarography, tagged atoms - as well as other physic-chemical procedures. (totalsoftwater.com)
  • This is a view dreamweaver cs5 in a various model( available system) whose section, solvers and spectroscopy is to Embed commonly from the non-equilibrium. (medmotion.com)
Behavior2
  • After purification dynamic light (DynaPro, Wyatt) scattering enable us to investigate the hydrodynamic behavior of the received sample to judge its suitability for crystallization. (hu-berlin.de)
  • Students will be versed in thermodynamical and rheological models of polymer systems and will be able to use them for prediction and interpretation of phase and flow behavior. (vscht.cz)
Probabilistic1
  • The method applies Bayesian probabilistic model to analyze scattering data from mixtures of oligomeric species, allows for a modeling large structural ensembles, can be used to assess uncertainty of all parameters and minimizes over-fitting. (lu.se)
Structural4
  • Homologous sequence comparison and structural modeling indicate CroV MCP should have a trimeric double-jelly-roll structure as seen in PBCV-1, another giant NCLDV. (utep.edu)
  • To overcome this problem we develop a method that combines a computational structural modeling (which delivers atomic-resolution structures) with experimental data (which provides information about the population of different states). (lu.se)
  • Using advanced detection capabilities, structural information is obtained from the intrinsic viscosity and hydrodynamic radius measurements. (malvernpanalytical.com)
  • These results were then correlated with small-angle X-ray scattering solution studies and computational tertiary structural models. (uleth.ca)
Membrane6
  • The performance of commonly used AEX resins and membrane adsorbers, detailing dynamic binding capacity performance, efficiency, and a new disposable option for packed bed chromatography are compared. (biopharminternational.com)
  • AEX chromatography resins and membrane adsorbers are frequently used for downstream purification in the biotechnology industry. (biopharminternational.com)
  • Multiple product formats are commercially available, including chromatography resins, which are packed into chromatography columns, and membrane adsorbers, which are supplied in self-contained plastic housings. (biopharminternational.com)
  • The scale-down model used to compare the performance of the different AEX products/formats is summarized in Table I. We evaluated five commonly used AEX products: three resins and two membrane adsorbers for dynamic binding capacity of DNA and protein using bovine serum albumin (BSA) to mimic common contaminants, such as host cell proteins, removed during a FT step. (biopharminternational.com)
  • For potential biomedical applications, several of synthesized hybrid polymers were investigated for their pore / channel activity in a lipid bilayer (a model membrane that serves as a good model for biomembrane). (gov.pk)
  • Only highly amphiphilic (PEG5k-b-P(MA-POSS)3.7 and P(POSS MA-co-S)8-b-P(ACM)695 with relatively small POSS content were found to have the ability to penetrate the model lipid bilayer membrane generating pores/channels. (gov.pk)
20203
  • 2020). Using Sentinel-1 Imagery to Assess Predictive Performance of a Hydraulic Model, Water Resources Management , Vol. 34, No. 14, pp. 4415-4430 ( https://doi.org/10.1007/s11269-020-02592-7 ). (ntua.gr)
  • 2020). Closure to "Friction Modeling of Flood Flow Simulations" Journal of Hydraulic Engineering , 146(5), art. (ntua.gr)
  • 2020). Reconstruction of a flash flood event using a 2D hydrodynamic model under spatial and temporal variability of storm, Natural Hazards , 101(3):711-726. (ntua.gr)
Flows2
  • In comparison to column chromatography, flows and total solvent usage can in most countercurrent chromatography separations may be reduced by half and even up to a tenth. (wikipedia.org)
  • The British Geological Survey produces a monthly Hydrological Outlook forecast for the UK, which detail conditions for river flows and groundwater levels, they explain, "The project has involved bringing together information on current and forecast weather conditions, soil moisture, river flows and groundwater levels, and uses a number of modelling methods to explore possible future hydrological conditions. (totalsoftwater.com)
Characterization1
  • Drug unbound fraction (ƒ u ) characterization is a key consideration in pharmacokinetic and pharmacodynamic (PK/PD) modeling, assuming only unbound drug can interact with the target, and therefore has direct implications in the efficacy and potential toxicity of the drug. (aspetjournals.org)
20192
  • 2019) Performance comparison of multiple and single surrogate models for pumping optimization of coastal aquifers, Hydrological Sciences Journal/Journal des Sciences Hydrologiques , 4(3):336-349 DOI: 10.1080/02626667.2019.1584400. (ntua.gr)
  • 2019), Comparison of Sharp Interface to Variable Density Models in Pumping Optimisation of Coastal Aquifers, Water Resources Management . (ntua.gr)
Detection2
Analyze3
  • Mass spectrum (MS) and dynamic light scattering (DLS) were performed to analyze the molecular weight and hydrodynamic diameter of the fusion protein. (utep.edu)
  • Gel Permeation Chromatography (GPC) can not only be used to separate and characterize small molecules but also be applied to analyze homologous series of polymers with same chemical properties but different molecular volume (polymers are separated by the column according to their hydrodynamic volume). (ms-labs.cn)
  • Viscotek's model TDA305 complete SEC/GPC system was used to analyze the samples. (malvernpanalytical.com)
Characteristics1
  • In the present paper, the disinfection characteristics of an advanced hydrodynamic cavitation reactor (ARHCR) in pilot scale were studied. (mostwiedzy.pl)
Variability1
  • This Small Business Technology Transfer Research (STTR) Phase I project aims to develop quantitative models of wind variability to aid the design of reliable, low-carbon electric grid systems with high wind penetration. (sbir.gov)
Approaches1
  • The work has also inspired other modelling approaches and prompted new lines of scientific research. (totalsoftwater.com)
Quantitative models1
  • The development of quantitative models for prediction of drug pharmacokinetics based on in vitro data has transformed early drug discovery. (aspetjournals.org)
Results1
  • The modelling results for 'a worst case scenario' of high EDTA release combined with a low river flow, suggest that the dairy effluent discharge at the Fonterra Waitoa dairy site will not lead to a significant effect on the Waitoa River in terms of EDTA concentration. (waikato.ac.nz)
Structures1
  • The large hydrodynamic size of the self assembled structures, as determined by the laser light scattering data, however, suggests that the aggregates are not of typical core-shell type micelles Second series consists of Poly(POSS-methacrylate-co-styrene)-b-Poly(acryloyl morpholine)x P(POSS-MA-co-S)x-b-P(ACM)y block copolymers which differs in chain length of polyacryloyl morpholine blocks. (gov.pk)
Numerical1
  • From this view dreamweaver cs5 randomly more media of class can use very been, numerical as total velocity breachesWipro or limited prediction model. (medmotion.com)
Performance3
  • Hydrodynamic instruments are often marketed as high-speed or high-performance countercurrent chromatography (HSCCC and HPCCC respectively) instruments which rely on the Archimedes' screw force in a helical coil to retain the stationary phase in the column. (wikipedia.org)
  • Comparing performance across product types and formats, including resins to membranes and membranes to membranes, is challenging due to the complicated and unique formats for scale-down models of the different product types. (biopharminternational.com)
  • The effectiveness of HC is determined by the performance of hydrodynamic cavitation reactors (HCRs). (mostwiedzy.pl)
Time1
  • Interaction effects between cancer history/time since diagnosis and predictors known to be associated with employment patterns, including age, gender/marital status, education, and health insurance status at baseline were modeled. (cdc.gov)
Role1
  • 18] She Q., Tang C.Y., Wang Y.N., Zhang Z., The role of Hydrodynamic Conditions and Solution Chemistry on Protein Fouling During Ultrafiltration , Desalination , 249 (3): 1079-1087 (2009). (nsmsi.ir)
Easily2
Lower2
  • On the other hand, the re-use of resins is well established for traditional packed bed chromatography and this facilitates lower overall material costs. (biopharminternational.com)
  • This means that the particle hydrodynamic interaction becomes more important at the lower volume fractions than the particle thermodynamic interaction. (dispersion.com)
Column3
  • Some types of countercurrent chromatography, such as dual flow CCC, feature a true countercurrent process where the two immiscible phases flow past each other and exit at opposite ends of the column. (wikipedia.org)
  • Standard column chromatography consists of a solid stationary phase and a liquid mobile phase, while gas chromatography (GC) uses a solid or liquid stationary phase on a solid support and a gaseous mobile phase. (wikipedia.org)
  • Model: Alliance e2695, HSPgel RT 4.0 (chloroform) column. (ms-labs.cn)
Unit1
  • The diffraction images collected from single crystals can be used to calculate the electron density map of the crystal unit cell which finally allows to model the three dimensional structure of the crystallized protein. (hu-berlin.de)
Unique2
  • Comparing resins with membranes and membranes to membranes is challenging due to the complicated and unique scale-down model formats of the products offered by different vendors. (biopharminternational.com)
  • Although the aftermath of DwH was studied intensely, lessons learned may not be directly transferable to other deep-sea hydrocarbon exploration areas, such as the Faroe-Shetland Channel (FSC) which comprises cold temperatures and a unique hydrodynamic regime. (elsevierpure.com)
Systems2
  • This Small Business Technology Transfer Research (STTR) Phase I project aims to improve predictive battery models and control systems for grid-scale energy storage applications. (sbir.gov)
  • For protein purification the lab is equipped with two chromatography systems (ÄKTA pure and ÄKTA express, GE Healthcare). (hu-berlin.de)
Technique2
  • Hydrodynamic cavitation (HC) has been widely considered a promising technique for industrial-scale process intensifications. (mostwiedzy.pl)
  • Hydrodynamic cavitation is a promising technique for water disinfection. (mostwiedzy.pl)
Potential1
  • Scope and approach: At this point, hydrodynamic cavitation (HC) is recognized as one of the potential technologies. (mostwiedzy.pl)
High2
  • Late-phase process redesign or a complete switch to packed bed chromatography may be required due to high consumable costs or limited product formats not allowing for linear scale-up. (biopharminternational.com)
  • Recently, the hydrodynamic cavitation (HC)-based pretreatment has shown high effectiveness in laboratories and even in industrial productions for conversion of lignocellulosic biomass (LCB) into value-added products. (mostwiedzy.pl)
Solid1
  • In reversed-phase chromatography, for example, the stationary phase can be regarded as a liquid which is immobilized by chemical bonding to a micro-porous silica solid support. (wikipedia.org)
Cancer1
  • The enhanced antitumor efficacy and reduced toxic effect of ethaselen-loaded polymeric micelle when compared with ethaselen-HP-β-CD inclusion were observed at the same dose in H 22 human liver cancer cell bearing mouse models. (springer.com)
Case1
  • Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. (elgalabwater.com)
Solutions1
  • Nearly any pair of immiscible solutions can be used in countercurrent chromatography provided that the stationary phase can be successfully retained. (wikipedia.org)
Process2
  • AEX products are used for polish chromatography in product FT mode to bind product-related and process impurities. (biopharminternational.com)
  • The advanced rotational hydrodynamic cavitation reactors (ARHCRs) that appeared recently have shown obvious advantages compared with conventional devices in process intensifications. (mostwiedzy.pl)
Study1
  • not, the Federal Reserve will Perhaps assess to prevent its Populist problem to evaluate property in metal, which will browse contagious general densities and could intensify further perspective study grains and arm model. (ournest.com)
Shown1
  • Recently we have shown that a coarse-grained model based on the primitive model, that has been used for modelling polyelectrolytes for over 30 years, works well for a range of IDPs where electrostatics governs the intra- and intermolecular interactions [1]. (lu.se)
Static1
  • SCM, static cluster model. (lu.se)