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  • proteins
  • A mixture containing one or more proteins of interest is dissolved in 100% buffer A and pumped into the column. (wikipedia.org)
  • Because the mass of individual peptides is measured in forming a fingerprint, mixtures of different proteins can yield unreliable results. (wikipedia.org)
  • If not isolated, the results will represent a mixture of two or more proteins and will therefore be unreliable in protein identification. (wikipedia.org)
  • They grew various yeasts on medium at different temperatures, then determined enzyme activity by separating proteins on a gel and fingerprinting the individual bands. (wikipedia.org)
  • protein
  • FPLC can be readily scaled from analysis of milligrams of mixtures in columns with a total volume of 5ml or less to industrial production of kilograms of purified protein in columns with volumes of many liters. (wikipedia.org)
  • In bio-informatics, a peptide-mass fingerprint or peptide-mass map is a mass spectrum of a mixture of peptides that comes from a digested protein being analyzed. (wikipedia.org)
  • gasoline
  • The refining process splits this mixture into other, more valuable mixtures such as natural gas, gasoline and chemical feedstocks, none of which are pure substances, but each of which must be separated from the raw crude. (wikipedia.org)
  • microporous
  • The stationary phases are usually finely ground powders or gels and/or are microporous for an increased surface, though in EBA a fluidized bed is used. (wikipedia.org)
  • poly
  • This invention relates to hydrolytically degradable gels of crosslinked poly(ethylene) glycol (PEG) structures. (google.com)
  • suitable
  • al were suitable for forming hard gels, including gels with chloroform, which had been resistant to gelation prior to their discovery. (wikipedia.org)
  • method
  • A method is provided for isolating and identifying a recombinant clone having a DNA segment therein coding for at least one desired heterologous polypeptide, at least a short amino acid sequence of which is known, by effecting cDNA synthesis on a mixture of mRNAs containing the mRNA coding for the desired. (google.com)
  • 5. The method of claim 1, wherein said target mRNA represents less than 2 mole percent of the total mRNA in the mixture. (google.com)
  • 6. The method of claim 1, wherein said target mRNA represents up to about 1 mole percent of the total mRNA in the mixture. (google.com)
  • molecules
  • Hydrolytic degradation can provide for dissolution of the gel components and for controlled release of trapped molecules, including drugs. (google.com)
  • organic
  • The addition of an acid to a mixture of an organic base and acid will result in the acid remaining uncharged, while the base will be protonated to form a salt. (wikipedia.org)
  • fractions
  • When used for analysis of mixtures the effluent is usually collected in fractions of 1-5 ml which can be further analyzed, e.g. by MALDI mass spectrometry. (wikipedia.org)
  • plates
  • Plates can be labeled before or after the chromatography process using a pencil or other implement that will not interfere or react with the process. (wikipedia.org)
  • Usually
  • This mixture is spread as a thick slurry on an unreactive carrier sheet, usually glass, thick aluminum foil, or plastic. (wikipedia.org)
  • molecular
  • Scanning Electron Microscopy is a useful means for researchers to determine the structural properties of a low molecular-mass weight gel. (wikipedia.org)
  • structural
  • entailed refluxing 3-bromophenol with bromoacetaldehyde and sodium hydride to give the diethyl acetyl, which then was heated with polyphosphoric acid to give a mixture of bromobenzofurans structural isomers: 4-bromo-1-benzofuran and 6-bromo-1-benzofuran. (wikipedia.org)
  • volume
  • Gels self-assemble through non-covalent interactions such as π-stacking, hydrogen-bonding, or Van der Waals interactions to form volume-filling 3D networks. (wikipedia.org)
  • chemical
  • A metalloid is any chemical element which has properties in between those of metals and nonmetals, or that has a mixture of them. (wikipedia.org)
  • result
  • The addition of an acid to a mixture of an organic base and acid will result in the acid remaining uncharged, while the base will be protonated to form a salt. (wikipedia.org)