Mixed lymphocyte cultures. Medical search. Frequent questions

Further characterization of the lymphocytes, accessory cells (dendritic cells, macrophage) and cytokines that participate in the MLR have been done as this assay continues to be used to define mechanisms for understanding cellular immune function in vitro. (wikipedia.org)
1964. Lymphocyte interaction: A potential histocompatibility test in vitro. (wikipedia.org)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
In vitro treatment of peripheral blood lymphocytes with TSO induced significant number of micronuclei in all samples. (scialert.net)
CTS™ Immune Cell Serum Replacement (SR) is designed to support expansion of in vitro cultured human immune cells such as T cells when added to a basal cell culture media such as CTS™ OpTmizer™ T cell Expansion SFM or CTS™ AIM-V™ SFM. (thermofisher.com)
The number and inhibitory activity of CD4(+)CD25(+)FoxP3(+) regulatory T cells were analysed by histology, flow cytometry and in vitro mixed lymphocyte cultures. (uni-koeln.de)
The potential of the test substance, manganese dichloride, to induce structural chromosomal aberrations in human lymphocyte cells in vitro was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guideline OECD 473. (europa.eu)
The test substance was therefore considered to be non-clastogenic to human lymphocytes in vitro. (europa.eu)
The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. (nature.com)

1984. Depression in B- and T-lymphocyte mitogen- induced blastogenesis in mice exposed to low concentrations of benzene. (cdc.gov)
Studies of lymphocyte mitogenic function in 45 exposed Michigan dairy farm ers showed normal responses to phytohemagglutinin (PHA) and pokeweed mitogen (PWM) and a reduced reactivity in mixed lymphocyte cultures in 27 subjects. (cdc.gov)
PHS supports Mixed Lymphocyte Culture (MLC) and Mitogen (PHA) Response. (labquip.net)

After several days, lymphocytes underwent blast transformation, DNA synthesis and cellular proliferation in response to the major histocompatibility antigen (MHC Class I and II) differences between the two cell populations designated as Responder and Stimulator cells. (wikipedia.org)
The membrane-active drug procaine inhibits the phagocytosis of latex particles by normal monocytes and the proliferation of lymphocytes in an allogeneic mixed leukocyte culture [ 3361 ]. (nanomedicine.com)
The bone marrow- and adipose tissue-derived MSCs were cultured under human platelet lysate conditions and their biological characteristics evaluated for cell therapy (morphology, immunophenotype, colony-forming unit-fibroblast efficiency, proliferation capacity, potential for mesodermal differentiation, secreted proteins, and immunomodulatory effects). (biomedcentral.com)
PTX and several cogeners were found to directly suppress T cell proliferation in response to phytohemagglutinin, to allogeneic cells in a mixed leukocyte reaction, and to cross-linking the CD3 complex. (elsevierpure.com)

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that help you meet your research needs and budget requirements. (thermofisher.com)

Under human platelet lysate-supplemented culture conditions, bone marrow- and adipose tissue-derived MSCs exhibited similar fibroblast-like morphology and expression patterns of surface markers. (biomedcentral.com)

Blocking antibody directed against donor responding cells has been found to develop in HL-A-mixed lymphocyte culture-identical donor-recipient combinations after renal transplantation in which splenectomy was not perfortned. (northwestern.edu)

The assay set-up consists of purifying responder lymphocytes from peripheral blood, thymus, lymph nodes or spleen and co-culturing with stimulator cells. (wikipedia.org)

Technically, it is an ex-vivo cellular immune assay that occurs between two allogeneic lymphocyte populations (same species but genetically distinct). (wikipedia.org)
In colorectal biopsies and peripheral blood lymphocytes of patients with IBD, polyps and CRC, we evaluated 1) genomic instability using the γH2AX assay as marker of DSB and micronuclei in mononuclear lymphocytes kept under cytodieresis inhibition, and 2) cellular stress through expression and cellular localization of glutathione-S-transferase omega 1 (GSTO1). (oncotarget.com)
The frequencies of chromosome aberrations in both vehicle and positive controls were within the expected range and verified the sensitivity of the assay and the efficacy of the S9-mix. (europa.eu)
Based on the results from the preliminary toxicity test, the doses selected for treatment of the initial mutagenesis assay ranged from 2.5 to 120 µg/mL and 20 to 160 µg/mL for the S9 non-activated and activated cultures, respectively. (europa.eu)
Based on the results of the preliminary toxicity test, the doses chosen for treatment of the extended treatment assay ranged from 0.31 to 15 µg/mL for non-activated cultures with a 24-hour exposure. (europa.eu)

Put simply, it is mixing populations of T-lymphocytes (category of white blood cells) together, and measuring the reaction that occurs. (wikipedia.org)
No MLR occurred when the Responder cells came from thymectomized animals, whereas bursectomized chicken leukocytes reacted in culture demonstrating that T-cells were the major cell type in Responder cell populations. (wikipedia.org)
Stimulator cell populations that also contain T-cells (Two way mixed lymphocyte reaction) will replicate in the presence of the Responder cells, therefore for a One way mixed lymphocyte reaction, stimulator cells are prevented from replicating by irradiation or treatment with mitomycin C, a DNA crosslinker to prevent cell replication. (wikipedia.org)
After a second centrifugation step, HBSS was removed and cells were resuspended in 5 mL culture medium and incubated for another 20 - 22 h (24 h fixation time). (europa.eu)
T cell preparation: Patients and donors undergo apheresis to obtain peripheral blood mononuclear cells (PBMCs), which are expanded in culture. (knowcancer.com)
Therefore a transwell co-culture model of human intestinal epithelial cells (IEC) and mixed lymphocytes was developed to study the cross-talk between these cells (see http://www.uu.nl/organisatie/3rs-centre-uls/3vs/3v-interviews ). (uu.nl)
Using this co-culture model, bacterial CpG DNA (a Toll like receptor 9 ligand) combined with NDO was found to effectively drive away from the allergic phenotype of the underlying immune cells by increasing epithelial release of galectin-9, a soluble type lectin known to neutralize IgE and instruct regulatory T-cells. (uu.nl)
The co-culture model is now further extended using immune cells of peanut or cow's milk allergic donors and NDO are now used as adjunct therapy in allergen specific oral or subcutaneous immunotherapy in food allergic mice (STW NUTRALL). (uu.nl)
Anomalous THY-1(+) killer cells in allogeneic and F1-anti-parental mixed leukocyte culture. (silverchair.com)
Anomalous killer cells are Thy-1(+) blasts that are cytolytic to the natural killer (NK)-sensitive lymphoma YAC-1, and that can be detected early (day 3-4) in the period preceeding the allospecific cytotoxic T lymphocyte (CTL) response in (CBA x A)F1 {arrow} C57B1 mixed leukocyte culture (MLC). (silverchair.com)
In such a combination, the AK response could be partially restored by the addition of irradiated +/nu (but not nu/nu) responder cells to the cultures. (silverchair.com)
During the study duplicate cultures of human lymphocyte cells, treated with test substance, were evaluated for chromosome aberrations over at least three dose levels. (europa.eu)
DC were found to be potent stimulators of T cells in allogeneic and, to a lesser extent, in autologous mixed leukocyte reaction (MLR). (amsterdamumc.org)
In six patients and six controls allogenic mixed leucocyte reaction (AMLR) was performed using mDC and cord blood-derived naive T cells at a DC/T naive ratio of 1 : 200. (torvergata.it)
Moreover, proliferative response of allogenic naive T cells cultured with mDC was impaired in the patients (13471 +/- 9917.2 versus 40976 +/- 24527.2 cpm, P = 0.04). (torvergata.it)
This study investigates the influence of the initial inflammatory stance of human mesenchymal stem cells (hMSCs) on key osteogenic, angiogenic, and immunomodulatory criteria when cultured in a class of mineralized collagen scaffolds under development for CMF bone repair. (bvsalud.org)

Lymphocytes (0.4 mL blood of a healthy male donor was added to 5 mL or 4.8 mL culture medium, without and with metabolic activation respectively and 0.1 mL (9 mg/mL) Phytohaemagglutinin) were cultured for 48 h and thereafter exposed to selected doses of test substance for 3 h, 24 h and 48 h in the absence of S9-mix or for 3 h in the presence of S9-mix. (europa.eu)
Three treatment conditions were used for the study, as follows: firstly, cultures were exposed for 4 hours with a 20 hour expression time, both in the presence and absence of metabolic activation (S9 mix), secondly cultures were continuously exposed for 24 hours in the absence of metabolic activation. (europa.eu)
Toxicity in the cloned cultures was observed at doses at 120 and 160 µg/mL without and with S9 activation, respectively. (europa.eu)

Why are Mixed Lymphocyte Reactions Performed? (wikipedia.org)
The purified BDC elicited strong allogeneic mixed leukocyte reactions and HLA classes II- and I-restricted antigen-specific primary immune responses. (nih.gov)
Experience with multiparameter flow cytometry and immune cell functions, such as mixed lymphocyte reactions. (postdocjobs.com)

Mixed lymphocyte reaction (MLR) is a test used by pharmaceutical and biotech organizations to show the safety of a drug or implantable material. (wikipedia.org)
1963. A reaction between leukocytes in mixed peripheral blood cultures. (wikipedia.org)

Cultured peripheral human lymphocytes were used as test system. (europa.eu)
Lymphocytes were cultured for 48 ± 2 h and thereafter exposed in duplicate to selected doses of test substance for 3 h in the absence and presence of S9-mix. (europa.eu)

The effect of antilymphocyte antibody on lymphocyte transformation. (eurekamag.com)
Similar results were obtained using biotinylated CMRF-56 mAb, an antibody identifying a comparable population in cultured PBMC. (nih.gov)
Cytotoxic antibody associated with rejection episodes and detected only by the discriminatory mixed lymphocyte culture developed in other HL-A-mixed lymphocyte culture-identical combinations as well. (northwestern.edu)

mDC, derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF) and stimulated with lipopolysaccharide (LPS) for the last 24 h, were phenotyped for the expression of the co-stimulatory molecules B7.1 and B7.2. (torvergata.it)

Notably, we found that a one-time licensing treatment of MSCs induced higher immunomodulatory potential compared to basal MSCs as observed by sustained immunomodulatory gene expression throughout the first 7 days as well as an increase in immunomodulatory cytokine (PGE2 and IL-6) expression throughout a 21-day culture period. (bvsalud.org)

Different media used in culture processes should maintain the biological characteristics of MSCs during multiple passages. (biomedcentral.com)
This study aims to conduct a direct head-to-head comparison of the biological characteristics of the two types of MSCs under human platelet lysate-supplemented culture conditions for their ability to be used in regenerative medicine applications. (biomedcentral.com)

Five patients had progressive disease, two patients had stable disease, and one patient experienced a partial response after receiving donor lymphocyte infusions and IFN-α. (aacrjournals.org)
Patients' PBMCs are irradiated and mixed with donor PBMCs. (knowcancer.com)

This study was taken up to evaluate the antigenotoxic potential of ginger in human peripheral blood lymphocytes. (scialert.net)
Peripheral human lymphocytes are recommended in international guidelines (OECD, EC). (europa.eu)
Gibco CTS OpTmizer T-Cell Expansion SFM supports the growth and expansion of human T lymphocytes. (thermofisher.com)

0.5 mL of whole blood in a total volume of 5 mL medium was cultured in the presence of 0.16 mM Trans Stilbene Oxide (TSO) for the induction of micronuclei. (scialert.net)
Micronuclei frequency began to rise in lymphocytes of subjects with polyps, suggesting a systemic genomic instability condition. (oncotarget.com)

All patients achieved initial mixed hematopoietic chimerism with two patients rejecting their graft and recovering host hematopoiesis. (aacrjournals.org)

Experience with DNA techniques and mammalian tissue culture. (postdocjobs.com)

Bidirectional mixed cultures were performed with lymphocytes from healthy controls and allergic patients with different time periods of IT, and the supernatants obtained from chromatography were tested to determine their effect on IgE synthesis of normal lymphocytes. (cun.es)
Suppressor factors derived from allergic patients had no effect on IgE synthesis of normal lymphocytes. (cun.es)

All of the positive control substances induced marked increases in the frequency of revertant colonies, confirming the activity of the S9-mix and the sensitivity of the bacterial strains. (europa.eu)

One hundred metaphase chromosome spreads per culture were examined by light microscopy for chromosome aberrations. (europa.eu)

Detect and identify Chlamydia by immunofluorescence technique after growth in tissue culture with Thermo Scientific™ Remel™ PathoDx™ Chlamydia Culture Confirmation Kit. (thermofisher.com)

Similar studies conducted in laboratory animals exposed to high and low doses of PBBs (not specified) found a dose related lymphocyte dysfunction in the high dose group. (cdc.gov)

Serum PBB concentrations, however, were not correlated with lymphocyte function. (cdc.gov)

A followup study of 90 Michigan residents with high or low PBB exposures found all parameters of lymphocyte function to be normal. (cdc.gov)
The author concludes that PBBs are capable of altering lymphocyte function. (cdc.gov)
The clinical significance of the changes in lymphocyte function are unknown. (cdc.gov)

In the other 18 subjects, the responses to PHA and PWM were significantly reduced along with that of mixed leukocyte cultures. (cdc.gov)

The MLR was first recognized when researchers mixed leukocytes from two unrelated donors in culture. (wikipedia.org)

This response became quantifiable when incorporating radioactive [3H] labelled thymidine or 5-bromo-2'-deoxyuridine (BrdU), into the mixed cell suspension. (wikipedia.org)
The adaptive immune response, primarily comprised of T and B lymphocytes, has important implications in the fracture healing process as well [ 3 , 4 ]. (hindawi.com)

Toxicity in the cloned cultures was observed at doses of 10 and 15 µg/mL. (europa.eu)

This OPXV strain (Italy_09/17) was isolated on cell cultures and embryonated eggs. (cdc.gov)

MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)

Gibco™ Water For Injection (WFI) for Cell Culture is high-quality water for use as a solvent in the preparation of cell culture media, laboratory reagents, buffers, and bioprocess liquids. (thermofisher.com)

The blood culture was also positive for Y. enterocolitica in 1 case. (who.int)

After 72 h, the cultures were processed and the cell suspension was fixed on slides and stained with 2% giemsa. (scialert.net)

It is a complete serum-free, xeno-free medium consisting of CTS OpTmizer T-Cell Expansion Basal Medium and CTS OpTmizer T-Cell Expansion Supplement, which are mixed together prior to use. (thermofisher.com)
In this study, mouse iPSCs were induced to differentiate into imDCs in culture medium without or with SN (iPSCs-imDCs and SN-iPSCs-imDCs). (newswise.com)

in stools by cold-enrichment culture at 4 ° C for 21 days. (who.int)

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