Mitotic cell cycle proteins. Medical search. Frequent questions

Consistent with these observations, we found that E4F localizes to the mitotic spindle during the M phase of early embryos. (cnrs.fr)
Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. (wikipedia.org)
Phosphorylates KIF11 to promote mitotic spindle formation. (wikipedia.org)
Any process that stops, prevents, or reduces the frequency, rate or extent of the movement of proteins from the cytoplasm into the nucleus, and that occurs as a response to the mitotic cell cycle spindle assembly checkpoint. (yeastgenome.org)
Glc7p functions in opposition to key spindle assembly checkpoint protein Aurora kinase (Ipl1p). (yeastgenome.org)
During the mitotic phase, mitotic chromosomes are attached to a structure known as the mitotic spindle. (differencebetween.com)
A centromere , the point on a chromosome that attaches to the spindle fibers with a kinetochore during cell division, attaches the sister chromatids. (coursehero.com)
The centrosome will organize a complex structure of microtubules , the mitotic spindle, involved in mitosis. (coursehero.com)
The researchers screened 20 solid cancer cell lines relative to gene silencing to identify a highly responsive chemo-resistant ovarian cancer cell line that underwent significant depletion in mitotic spindle-dynamics for effective experimental cancer treatment. (medicalxpress.com)
Dynamin 2 also clings to microtubules of the mitotic spindle, and the team next wants to determine whether the protein regulates microtubule dynamics during the cell cycle. (rupress.org)
The fission yeast polo related kinase Plo1 is required to assemble the mitotic spindle, the prophase actin ring that predicts the site for cytokinesis and for septation after the completion of mitosis (Ohkura et al. (kent.ac.uk)
1998). We show that Plo1 associates with the mitotic but not interphase spindle pole body (SPB). (kent.ac.uk)
SPB association is strong from mitotic commitment to early anaphase B, after which the Plo1 signal becomes very weak and finally disappears upon spindle breakdown. (kent.ac.uk)
These checkpoints may stop the cell cycle after DNA damage, loss of DNA replication or disruption of the mitotic spindle, in order for repair processes to take place. (tocris.com)

Our results establish a crucial role for E4F during early embryonic cell cycles and reveal an unexpected function for E4F in mitosis. (cnrs.fr)
Progression through mitosis depends on a large number of protein complexes that regulate the major structural and physiological changes necessary for faithful chromosome segregation. (nih.gov)
Analysis of the mcd1 mutant and cell cycle-dependent expression pattern of Mcd1p suggest that this protein functions in chromosome morphogenesis from S phase through mitosis. (nih.gov)
Interphase and mitosis are two important phases of cell division . (differencebetween.com)
Interphase is the longest phase of cell division, and mitosis is the shortest phase of cell division. (differencebetween.com)
Mitotic chromosomes will undergo different biochemical changes under four sub-stages of mitosis. (differencebetween.com)
We find that NF45 and NF90 play essential roles in mitosis, and transcriptome analysis reveals that they are necessary for the expression of a subset of mitotic mRNAs. (iric.ca)
We introduced autocatalytic transcription of E2F, E2F regulated transcription of cyclin B, Cdc20/Cdh1 mediated E2F degradation, enhanced transcription of mitotic cyclins during late S/early G2 phase, and the sustained synthesis of cyclin B during mitosis. (ebi.ac.uk)
A fifth phase has been identified, G0, in which the cell is resting just after mitosis but still carrying out normal functions. (coursehero.com)
The M phase consists of mitosis, in which the cell divides, producing two new, identical cells. (coursehero.com)
Interphase is collectively the gap 1 (G1), synthesis (S), and gap 2 (G2) phases of mitosis, in which a cell grows, replicates its DNA, and grows again. (coursehero.com)
Other cellular structures are duplicated during G2, such that each replicated daughter cell produced during mitosis will have all necessary organelles (such as mitochondria, endoplasmic reticulum, etc. (coursehero.com)
Colchicine prevents the mitosis of the cells at which of the following stage? (zigya.com)
Many chemotherapeutic agents explore defects in the cell cycle machinery of cancer cells to halt the cycle through mitosis inhibition. (medicalxpress.com)
However, existing mitosis-targeting chemotherapeutic agents do not discriminate between healthy and malignant cell lines, resulting in severe side-effects . (medicalxpress.com)
The challenge therefore is to identify molecular targets associated with mitosis of cancer cells. (medicalxpress.com)
Cell mitosis is an attractive target to effectively treat a variety of cancer forms, where microtubule-associated proteins (MAPs) assist cells to maintain the stability of cell dynamics. (medicalxpress.com)
Additionally, 65 proteins were detected on the chromosomal periphery during mitosis, and these could be further divided into two recruitment phenotypes with different temporal profiles. (kth.se)
Additionally, we detected hundreds of proteins with previously unknown relations to mitosis and the cell cycle, many being linked to proliferation and oncogenic functions. (kth.se)
During the "M phase" of the cell cycle (mitosis) Geminin stabilizes the replication factor Cdt1 promoting DNA replication during the next cell cycle. (prospecbio.com)
Moreover, inhibition of Geminin during mitosis (by RNAi) causes destabilization of Cdt1 protein and impairment of DNA replication during the next cell cycle. (prospecbio.com)
The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis. (wikidata.org)

Embryos lacking E4F die at the peri-implantation stage, while in vitro-cultured E4F(-/-) blastocysts exhibit defects in mitotic progression, chromosomal missegregation, and increased apoptosis. (cnrs.fr)
It is a protein kinase which plays an important role in mitotic cell cycle progression. (wikipedia.org)
Mouse emi1 has an essential function in mitotic progression during early embryogenesis. (nih.gov)
During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. (genetex.com)
Progression of a cell through the division cycle is tightly controlled at different steps to ensure the integrity of genome replication and partitioning to daughter cells. (ebi.ac.uk)
Important regulator of cell cycle progression. (neobiotechnologies.com)
Checkpoint control kinases is a term used to describe a group of enzymes that regulate progression of a cell through the cell cycle. (tocris.com)
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. (cancerindex.org)

SIRT1 is localized in the nucleus and can deacetylate numerous proteins such as tumor suppressor protein (p53), Ku70, NF-κB, and forkhead proteins which modulate genes that control cellular stress resistance ( Smith, 2002 ). (frontiersin.org)
The S. cerevisiae MCD1 (mitotic chromosome determinant) gene was identified in genetic screens for genes important for chromosome structure. (nih.gov)
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. (thebiogrid.org)
In human cells, the expression of ∼1,000 genes is modulated throughout the cell cycle. (iric.ca)
The protein is associated with microtubules , and Chatterjee and colleagues silenced the gene via short interfering RNA (siRNA) a molecular biological mechanism to study genes, targeting the CKAP5 encapsulated in lipid nanoparticles for in vivo delivery. (medicalxpress.com)
In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)
In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. (tocris.com)

Microtubules connect with the centrosomes of the lined up mitotic chromosomes. (differencebetween.com)
During the G2 phase, a structure in the cytoplasm of animal cells that coordinates the formation of microtubules, called a centrosome , allows cell division to proceed during reproduction. (coursehero.com)
A collection of microtubules (structural proteins) called a centrosome also replicates. (coursehero.com)
One such protein is the cytoskeleton-associated protein 5 (CKAP5), widely expressed in a variety of cells to regulate the dynamics of microtubules in human cells. (medicalxpress.com)
A mutant protein makes the microtubules too stable to perform their jobs, the researchers find. (rupress.org)
One of these mutations leads to production of faulty dynamin 2, a protein that is crucial for endocytosis but also latches onto microtubules. (rupress.org)
But microtubules from cells that made the faulty version of dynamin 2 were abnormally stable, as measured by how many acetyl groups were attached to them. (rupress.org)
Kinesin-8s are dual-activity motor proteins that can move processively on microtubules and depolymerize microtubule plus-ends, but their mechanism of combining these distinct activities remains unclear. (nature.com)

Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. (nih.gov)
During anaphase, the mitotic chromosomes (sister chromosomes) split evenly. (differencebetween.com)

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
If cells containing damaged DNA were to divide, the errors would be transmitted to daughter cells, generating genomic instability and resulting in tumorigenesis or apoptosis . (tocris.com)
This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. (cancerindex.org)
Low levels of pyruvate induced by a positive feedback loop protects cholangiocarcinoma cells from apoptosis. (cancerindex.org)

The period of the cell cycle when CELLS divide their nucleus and cytoplasm. (bvsalud.org)
The cells are usually large and polygonal, containing abundant eosinophilic cytoplasm, a large nucleus with open chromatin, a prominent eosinophilic nucleolus, and an occasional intracellular lumina containing red blood cells 1,4-7 . (bvsalud.org)

The mechanism, which is based on the synthesis and degradation of three ''master regulator'' proteins (CtrA, GcrA, and DnaA), is converted into a quantitative model, in order to study the temporal dynamics of these and other cell cycle proteins. (ebi.ac.uk)
Geminin has 2 roles in 2 different stages of the cell cycle: Geminin is a negative regulator of DNA replication during the "S phase" of the cell cycle. (prospecbio.com)

3409. V.C. Cordes, H.R. Rackwitz, S. Reidenbach, 'Mediators of nuclear protein import target karyophilic proteins to pore complexes of cytoplasmic annulate lamellae,' Exp. (nanomedicine.com)

In a subsequent in-depth analysis of key mitotic regulatory complexes with the Aurora kinase B (AURKB) inhibitor Hesperadin and a new Polo-like kinase (PLK1) inhibitor, BI 4834, we determined the kinase dependency for 172 phosphorylation sites on 41 proteins. (nih.gov)
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. (neobiotechnologies.com)

The accumulation of both Cyclin and CKI proteins is tightly regulated at the level of transcription. (intechopen.com)
In addition, Cyclin and CKI proteins are controlled at the level of their destruction. (intechopen.com)
Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on HeLa cell. (novusbio.com)
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (novusbio.com)
This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. (novusbio.com)
This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. (novusbio.com)
This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. (genetex.com)
WB analysis of HeLa cell lysate using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
IHC-P analysis of human mantle cell lymphoma tissue using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
The model depends on Cdh1-regulated cyclin degradation during G1, regulation of B cyclin/Cdk1 activity by cyclin A/Cdk via Wee1, and transcriptional control of the mitotic cyclins that reflects some of the current literature. (ebi.ac.uk)
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes. (wikidata.org)

The physical association between Mcd1p and Smc1p, one of the SMC family of chromosomal proteins, further suggests that Mcd1p functions directly on chromosomes. (nih.gov)
While interphase chromatin appears as thread-shaped structures, mitotic chromosomes appear as distinct rod-shaped structures. (differencebetween.com)
What are Mitotic Chromosomes? (differencebetween.com)
Mitotic chromosomes are distinct rod-shaped, highly condensed chromosomes that are present in the mitotic phase of cell division. (differencebetween.com)
Mitotic chromosomes are very distinct due to the shape and the amount of condensation. (differencebetween.com)
Mitotic chromosomes line up at the metaphase plate. (differencebetween.com)
Interphase chromatin and mitotic chromosomes are types of chromosomes. (differencebetween.com)
Moreover, interphase chromatins are less condensed, while mitotic chromosomes are heavily condensed. (differencebetween.com)
The below infographic presents the differences between interphase chromatin and mitotic chromosomes in tabular form for side by side comparison. (differencebetween.com)
Mitotic chromosomes appear as distinct rod-shaped structures, and they are heavily condensed. (differencebetween.com)
Both interphase chromatin and mitotic chromosomes are essential for the cycle of cell division. (differencebetween.com)
So, this is the summary of the difference between interpahse chromatin and mitotic chromosomes. (differencebetween.com)
Interphase is characterized by cell growth and DNA replication , while M is the phase of the cell cycle in which the chromosomes of the cell prepare for the cell to divide into two new cells. (coursehero.com)
In eukaryotic cells , DNA is found in structures called chromosomes. (coursehero.com)
3412. L.G. Koss, 'Characteristics of chromosomes in polarized normal human bronchial cells provide a blueprint for nuclear organization,' Cytogenet. (nanomedicine.com)
For a cell to progress through the cycle and replicate, it must pass through checkpoints between phases to ensure that DNA is replicated correctly and that chromosomes segregate. (tocris.com)

Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)

Treatment of HeLa cells with paclitaxel induces phosphorylation of Aurora A at Thr288 while treatment with hydroxyurea ablates phosphorylation. (cellsignal.com)

Avoid freeze-thaw cycles. (novusbio.com)
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. (prospecbio.com)

The activity of CDKs is controlled by their binding to coactivator subunits termed Cyclins, as well as by CDK inhibitory proteins termed CKIs. (intechopen.com)
Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (novusbio.com)

The key difference between interphase chromatin and mitotic chromosome is the structural appearance of the chromatin when observed under the microscope. (differencebetween.com)
Interphase chromatin is a thread-shaped chromosome that is present in the interphase of cell division. (differencebetween.com)
These thread-shaped structures are present at the interphase for a longer time accumulating nutrients, synthesizing proteins, and generating new organelles. (differencebetween.com)
At the end of the interphase, the cell becomes ready to process into the mitotic stage. (differencebetween.com)
During condensation, an SMC protein known as condensin binds to a single interphase chromatin at different locations, twisting the chromatin to different coils and lops. (differencebetween.com)
This occurs at the interphase of the cell cycle with the involvement of condensin, an SMC protein. (differencebetween.com)
The G1, S, and G2 phases of the cell cycle are collectively known as interphase. (coursehero.com)
A cell spends most of its time in interphase, during which it mostly performs its normal functions, such as protein synthesis. (coursehero.com)
Interphase comprises the gap 1 (G1), synthesis (S), and gap 2 (G2) phases, in which the cell grows and replicates its genetic material. (coursehero.com)
Interphase, the part of the cell cycle between division events, includes the G1, S, and G2 phases, during which the cell grows, replicates its DNA, and undergoes its usual functions. (coursehero.com)
Interphase takes most of the time in the cell cycle, comprising more than 95% of the duration of the cell cycle in most eukaryotes. (coursehero.com)
During interphase, a cell grows larger. (coursehero.com)
1990). Significantly, Plo1 associates inappropriately with the interphase SPB of stf1.1 cells. (kent.ac.uk)

The ubiquitously expressed E4F protein was originally identified as an E1A-regulated cellular transcription factor required for adenovirus replication. (cnrs.fr)
They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. (novusbio.com)
The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. (wikidata.org)

Most, if not all, of the mitotic processes are regulated by a set of mitotic protein kinases that control protein activity by phosphorylation. (nih.gov)
Although many mitotic phosphorylation events have been identified in proteome-scale mass spectrometry studies, information on how these phosphorylation sites are distributed within mitotic protein complexes and which kinases generate these phosphorylation sites is largely lacking. (nih.gov)
NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. (wikipedia.org)
A mitotic cascade of NIMA family kinases. (wikipedia.org)
Polo kinases execute multiple roles during cell division. (kent.ac.uk)
Checkpoint control kinases function to halt the cell cycle at these critical points. (tocris.com)

In addition, Geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions. (prospecbio.com)

3419. M.A. Mancini, D. He, I.I. Ouspenski, B.R. Brinkley, 'Dynamic continuity of nuclear and mitotic matrix proteins in the cell cycle,' J. Cell Biochem. (nanomedicine.com)
3422. J.H. Hughes, M.B. Cohen, 'Nuclear matrix proteins and their potential applications to diagnostic pathology,' Am. J. Clin. (nanomedicine.com)

Protein and gene expression of apoptotic signaling pathway-related proteins such as caspase-3, cytochrome c, and PARP were determined to provide evidence for the mechanisms of action of Tan IIA in the treatment of psoriasis. (hindawi.com)

In S. cerevisiae, this process involves inhibition of the karyopherin/importin Kap121p (also known as Pse1p), which acts as the specific nuclear import receptor for several proteins, including Glc7p. (yeastgenome.org)
The product of the nudE gene isolated in the screen, NUDE, is a homologue of the nuclear distribution protein RO11 of Neurospora crassa. (xenbase.org)
3407. S.A. Rutherford, M.W. Goldberg, T.D. Allen, 'Three-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures,' Exp. (nanomedicine.com)
3418. P. Loidl, A. Eberharter, 'Nuclear matrix and the cell cycle,' Int. Rev. Cytol. (nanomedicine.com)
Geminin is a 25 kDa nuclear protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. (prospecbio.com)
Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)

The function of this protein in normal cell physiology remains largely unknown. (cnrs.fr)
The model accounts for important details of the physiology, biochemistry, and genetics of cell cycle control in stalked C. crescentus cell. (ebi.ac.uk)

We used systematic protein-affinity purification combined with mass spectrometry to identify 1818 phosphorylation sites in more than 100 mitotic protein complexes. (nih.gov)
Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map. (thebiogrid.org)
however, even knowledge of the stoichiometry, affinity and lifetime of every protein-protein interaction would not reveal the functional relationships between and within such complexes. (thebiogrid.org)
Drosophila MCM protein complexes. (colorado.edu)

Involved in G2/M phase cell cycle arrest induced by DNA damage. (wikipedia.org)
Treatment of neuroblastoma PDX cells with ARRY-520 resulted in mitotic arrest and subsequent cell death. (lu.se)
Rigosertib reduced cell viability of neuroblastoma cells and caused cell cycle arrest in the G2/M phase. (lu.se)

Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)

Protein phosphorylation is one of the widely used posttranslational modifications that alter protein function in vivo. (stanford.edu)
Human fibroblast monocultures were found to be insufficient to fully replicate in vivo fibrosis outcomes suggesting in vitro predictive potential depends upon more advanced cell culture in vitro models. (cdc.gov)

Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
In particular, ubiquitin-mediated degradation is critically important at transition points where it provides directionality and irreversibility to the cell cycle, which is essential for maintaining genome integrity. (intechopen.com)

The LIS1 -related NUDF protein of Aspergillus nidulans interacts with the coiled-coil domain of the NUDE/RO11 protein. (xenbase.org)
NUDF protein interacts with the Aspergillus NUDE coiled-coil in a yeast two-hybrid system, while human LIS1 interacts with the human homologue of the NUDE/RO11 coiled-coil and also the Xenopus MP43 coiled-coil. (xenbase.org)

Paper IV presents a systematic dissection of the variable proteome drafted in Paper I. We show evidence for 539 proteins being correlated to cell cycle variations, of which a minority are also cycling at a transcriptional level, suggesting protein regulation at a translational or post-translational level. (kth.se)

Histone proteins are basic proteins used for the packaging of eukaryotic DNA. (zigya.com)
During S- phase of cell cycle, synthesis of histone proteins takes place as the chromosome number gets doubled to that of somatic number. (zigya.com)
The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. (cancerindex.org)

NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay. (iric.ca)

Involved in regulation of mitotic cell cycle. (nih.gov)
A Data-Driven, Mathematical Model of Mammalian Cell Cycle Regulation. (ebi.ac.uk)
To develop a data-driven model of cell cycle regulation, we used contiguous, dynamic measurements over two time scales (minutes and hours) calculated from static multiparametric cytometry data. (ebi.ac.uk)
Acts upstream of or within cellular response to DNA damage stimulus and positive regulation of G1/S transition of mitotic cell cycle. (nih.gov)

We recently showed phosphorylation of Drosophila Plexin A by cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and subsequent inhibition of plexin-mediated repulsive guidance. (stanford.edu)

During telophase, the new cell contents start to form at the two ends, forming two new cells followed by cytokinesis. (differencebetween.com)

This phosphorylation occurs in the active site of the plexin GTPase-activating protein (GAP) domain, which in turn inhibits endogenous GAP activity toward Ras/Rap family small GTP-binding proteins by recruiting the phospho-serine/threonine-binding protein 14-3-3ε. (stanford.edu)

This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. (genetex.com)
For this work, I have used patient-derived xenograft mouse models and neuroblastoma cells grown as 3D tumor organoids. (lu.se)
Analysis of KSP expression across multiple tumor types demonstrated that neuroblastoma is highly dependent on this protein. (lu.se)
therefore, definitive differentiation of Hürthle cell carcinoma from Hürthle-cell adenoma is based on vascular invasion and/or capsular invasion, as well as on permanent histologic sections or extrathyroidal tumor spread and lymph node and systemic metastases. (medscape.com)

To characterize the change(s) in mRNA and protein expression of SIRT1-7 and their associated proteins in the brain of "physiologically" aged Wistar rats. (frontiersin.org)
We tested mRNA and protein expression levels of rat SIRT1-7, and the levels of associated proteins in the brain using RT-PCR and western blotting. (frontiersin.org)
We used the autologous uterine tissue transfer mouse model and studied the mRNA and protein expression of ERα, ERβ, GPER1, and PR in ectopic lesions at 2, 4, and 8 weeks of induction of endometriosis. (springer.com)

SPB association of Plo1 is the earliest fission yeast mitotic event recorded to date. (kent.ac.uk)
cdc25+ functions as an inducer in the mitotic control of fission yeast. (wikidata.org)
The fission of a CELL. (bvsalud.org)

Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix. (neobiotechnologies.com)

We present a model for mitotic chromosome structure that incorporates this previously unsuspected link. (nih.gov)

We show that depletion of SMD components increases the binding of mitotic mRNAs to the NF45-NF90 complex and rescues cells from mitotic defects. (iric.ca)

A similar coiled-coil domain is present in several putative human proteins and in the mitotic phosphoprotein 43 ( MP43 ) of X. laevis. (xenbase.org)

and the M phase, the fourth phase of the cell cycle, when a cell divides. (coursehero.com)
A cell can remain in the G0 phase indefinitely, awaiting a signal to return to the normal cell cycle. (coursehero.com)
The amount of time spent in any given phase of the cell cycle is dependent upon the type of cell and the environmental conditions in which the cell lives. (coursehero.com)
The length of this particular phase is the most variable among different cell types. (coursehero.com)
When the cell receives signals for division, it moves to the S phase. (coursehero.com)
Once the DNA has been replicated, the cell moves to the second gap phase. (coursehero.com)
The rapid protocol (RP) PathScan ® RP Phospho-Aurora A (Thr288) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Aurora A protein phosphorylated at Thr288 in a reduced assay time of 1.5 hours. (cellsignal.com)
Catastrophic genetic damage can occur if cells progress to the next phase of the cell cycle before the previous phase is properly completed. (tocris.com)

Because of its mutated aggressive genetics, this cell has a selective growth advantage over its neighbors. (medscape.com)

It is highly specific and shows no cross-reaction with other related mitotic inhibitors. (neobiotechnologies.com)
The inhibitors demonstrated promising effects in vitro with reduced viability, increased differentiation and cell death of neuroblastoma cells. (lu.se)

I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
I further investigated membrane proteins and the targeting of tail-anchored proteins at Sheffield Hallam University. (brookes.ac.uk)

Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-Aurora A (Thr288) in a single step. (cellsignal.com)
In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. (neobiotechnologies.com)

J Cell Biol, 2007 Jan 1. (nih.gov)
Mol Cell Biol, 2006 Jul. (nih.gov)
J Cell Biol (2009) 185 (6): 930. (rupress.org)
J. Cell Biol. (rupress.org)

The relationship between lysate protein concentration from paclitaxel-treated and hydroxyurea-treated HeLa cells and the absorbance at 450 nm using the PathScan ® RP Phospho-Aurora A (Thr288) Sandwich ELISA Kit #41333 is shown in the upper figure. (cellsignal.com)
HeLa cells were treated with paclitaxel (100 nM) or hydroxyurea (4 mM) for 18 hr at 37°C and then lysed. (cellsignal.com)
HeLa or MCF7 cells. (neobiotechnologies.com)

A lack of high-resolution structures capturing a kinesin-8 motor domain at each major step of its motile and microtubule depolymerization cycles has limited our understanding of the relationship between these two catalytic cycles and the structural elements of the motor domain that control them. (nature.com)

An image depicting head and neck squamous cell carcinoma in vitro can be seen below. (medscape.com)
Head and neck squamous cell carcinoma in vitro (cell culture). (medscape.com)
We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)

The centromere is aided in binding sister chromatids together by several proteins called cohesins and condensins. (coursehero.com)

Using proteomics, we identify protein clusters associated with the NF45-NF90 complex, including components of Staufen-mediated mRNA decay (SMD). (iric.ca)

A monomorphous cell population of Hürthle cells arranged in loosely cohesive clusters and single cells. (medscape.com)

This protease activity has recently been ascribed to serine metalloprotease(s), including pregnancy-associated plasma protein-A (PAPP-A), which was first detected in human follicular fluid nearly 20 yr ago. (bioone.org)
I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
Kolkova Z, Noskova V, Ehinger A, Hansson S, Casslén B. G protein-coupled estrogen receptor 1 (GPER, GPR 30) in normal human endometrium and early pregnancy decidua. (springer.com)
Orthologous to human FBXO5 (F-box protein 5). (nih.gov)
This technologic and biologic revolution continued through the 1960s to today, and the explosion in technology has fueled the current expansion of knowledge into the working of the human cell. (medscape.com)
Cy5 is labeled as magenta, and the cell membrane is stained with rabbit anti-human epidermal growth factor receptor and goat anti rabbit488 antibody. (medicalxpress.com)
NUDF protein, the product of the nudF gene, displays 42% sequence identity with the human protein LIS1 required for neuronal migration. (xenbase.org)
B) Complementation of the nudE deletion and the nudF7 mutant by extra copies of nudE, the nudE NH2-terminal domain, and nudE chimeras carrying coiled-coil regions from human and frog proteins, respectively. (xenbase.org)
Using an antibody-based imaging approach, Paper I contains a subcellular map of 12 003 protein in 30 different cellular structures, freely accessible as part of the Human Protein Atlas (www.proteinatlas.org). (kth.se)
Apart from enabling exploration of the organellar proteomes, we conclude that half of the human proteins localize to multiple compartments, and that almost one fifth display cell-to-cell variations in terms of protein expression. (kth.se)
Anti-human Geminin mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Geminin amino acids 1-209 purified from E. coli. (prospecbio.com)
To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. (molvis.org)
One-hundred and twenty-seven of 17,225 human full-length proteins were identified that interact with CRYAA. (molvis.org)
The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)

The therapeutic potential of microtubule-associated protein targets for cancer therapy is a largely unexplored research area due to a lack of target-specific agents. (medicalxpress.com)
At this time, our knowledge of the catalytic cycles of kinesins is limited to motile kinesins that are incapable of microtubule depolymerization, and to microtubule-depolymerizing kinesins that are non-motile. (nature.com)

Combination of the results of the cellular studies with Scansite motif prediction enabled us to identify 14 sites on six proteins as direct candidate substrates of AURKB or PLK1. (nih.gov)
The purpose of this study was to determine the ontogeny of cellular changes in the expression of estrogen receptors (ERα, ERβ), G protein-coupled estrogen receptor 1 (GPER1), and progesterone receptors (PRs) in endometriosis using a mouse model. (springer.com)
This is the multihit theory of tumorigenesis, in which a series of multiple triggering events in the genetic and cellular makeup of a cell ultimately cause cancer. (medscape.com)
The small protein ubiquitin plays a vital role in virtually all aspects of cellular life. (intechopen.com)
In other words, determining the cellular location of proteins over time. (kth.se)
Oncocytic cells in the thyroid are often called Hürthle cells, and oncocytic change is defined as cellular enlargement characterized by an abundant eosinophilic granular cytoplasm as a result of accumulation of altered mitochondria. (medscape.com)

My additional independent research on ER localisation and splicing in auxin biosynthesis showed for the first time ER-localisation for an auxin biosynthetic protein. (brookes.ac.uk)

Anatomy35

Organisms17

Diseases4

Chemicals and Drugs104

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes83

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science4

Health Care2

Spindle14

Mitosis22

Progression11

Genes7

Microtubules8

Anaphase2

Apoptosis10

Nucleus2

Regulator2

Cytoplasmic1

Inhibitor2

Cyclin11

Chromosomes16

Proliferation2

Induces1

Freeze-thaw cycles2

Cyclins2

Interphase13

Transcription3

Kinases6

Embryogenesis1

Matrix proteins2

Signaling pathway-related proteins1

Nuclear7

Physiology2

Complexes4

Arrest3

Pathway1

Vivo2

Degradation2

Interacts2

Transcriptional1

Histone3

Regulate1

Regulation4

Inhibition1

Cytokinesis1

Inhibits1

Tumor4

MRNA and protein expression3

Fission3

Hepatocellular carcinoma1

Chromosome structure1

Defects1

Phosphoprotein1

Phase8

Genetics1

Inhibitors2

Membrane proteins2

Lysates2

Biol4

HeLa3

Structural1

Vitro3

Several proteins1

Proteomics1

Clusters1

Human15

Microtubule2

Cellular6

Biosynthesis1