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  • MEIOSIS
  • Adult male mice received MET (3.75 or 5 mg/kg-bw/ip/day/4 days) and were euthanized 1, 28 or 45 days post-treatment (dpt) to evaluate MET's effects on epididymal maturation, meiosis or mitosis, respectively. (osti.gov)
  • Data suggest that meiosis and mitosis are the more sensitive stages of spermatogenesis for MET reproductive toxicity compared to epididymal maturation. (osti.gov)
  • Methamidophos causes sperm DNA damage at mitosis, meiosis and epididymal maturation. (osti.gov)
  • Mechanisms of structural disintegration of microtubules by Katanin involve heterogeneous mechanochemical processes and sensitivity of microtubules to Katanin plays significant roles in mitosis/meiosis, neurogenesis, cilia/flagella formation, cell wall development and so on. (hindawi.com)
  • progression
  • An extensive cross talk exists between phosphorylation and other posttranslational modifications, which together regulate various biological processes, including gene transcription, DNA repair, and cell cycle progression. (asm.org)
  • However, the generality of the requirement for the phosphorylation of histone H3 for chromosome condensation and segregation must be questioned by the finding that budding yeast cells in which serine 10 of histone H3 is replaced with alanine show no apparent defects in cell cycle progression or chromosome transmission. (rupress.org)
  • Recent work has highlighted that regulating the phosphatases that revert CDK phosphorylations is as important as regulating the CDKs for cell cycle progression. (mdpi.com)
  • posttranslational
  • Although discovered long ago, posttranslational phosphorylation of histones has been in the spotlight only recently. (asm.org)
  • Recent mass spectrometry data have revealed that the histone globular domains are also modified and that many residues which had previously been implicated in gene expression through genetic screens in yeast ( 71 , 98 ) are targets for posttranslational modifications. (asm.org)
  • amino
  • Histones can be simultaneously modified in more ways than one, at various amino acid residues. (asm.org)
  • Using amino acid substitution combined with live cell imaging, time-lapse microscopy and FRAP, we investigated the role of serine 1880 in binding of gp210 to the NPC in vivo An alanine subtitutions mutant (S1880A) was significantly more dynamic at the NPC compared to the wild-type protein, suggesting that serine 1880 is important for binding of gp210 to the NPC. (diva-portal.org)
  • The substrate motif targeted by NEK2 for phosphorylation shows the strongest amino acid selectivity in the -3 and +2 positions (relative to the Ser/Thr residue that is phosphorylated by NEK2). (eu.org)
  • The occurrence of one of these residues, which are highly preferred to other hydrophobic residues, might compensate for less favored amino acids in the +1 and +2 positions. (eu.org)
  • In the -2 position, all amino acids except Pro are tolerated, although there is a slight preference for basic and hydrophobic residues. (eu.org)
  • Although there seems to be a preference for particular hydrophobic residues in the +1 position and for specific amino acids in the +2 position, the occurrence of less favored residues in these two positions is allowed in this variant of the motif, as this might be compensated by the presence of the strongly selected Phe, Leu or Met in the -3 position. (eu.org)
  • Phosphorylations of the both two amino acids, threonine308 (Thr308) and serine473 (Ser473) have been considered to be essential for Akt activation. (go.jp)
  • An amino acid sequence in the vicinity of serine/threonine, which is phosphorylated by this Akt, has been known as RXRXXS/T (J. Biol. (go.jp)
  • inhibition
  • The conserved Greatwall-Endosulfine pathway has been shown to be required for PP2A-B55 inhibition at mitosis in yeasts and multicellular organisms. (mdpi.com)
  • It has known that the activated Akt promotes the phosphorylation of the molecules involved in the apoptosis inhibition. (go.jp)
  • methylation
  • H3 serine28 phosphorylation (H3S28ph), generated by MITOGEN- AND STRESS-ACTIVATED PROTEIN KINASE1, not only prevents recruitment of the gene-silencing Polycomb-repressive complexes and their methylation of H3K27, but it also induces a switch from methylation to acetylation at H3K27 ( Lau and Cheung, 2011 ). (plantcell.org)
  • For established targets we seek to add antibodies that recognize new epitopes, including post-translational modifications such as phosphorylation and methylation. (abgent.com)
  • promotes
  • CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. (abcam.com)
  • Together, our results provide insight into how phosphorylation of H2A serine 95 promotes flowering time and suggest that phosphorylation of H2A serine 95 modulated by MLK4 is required for the regulation of flowering time and is involved in deposition of the histone variant H2A.Z and H4 acetylation in Arabidopsis. (plantcell.org)
  • checkpoint
  • Therefore, the partitioning of the Golgi complex is a necessary event for the cell to enter into mitosis, indicating that a 'Golgi checkpoint' can sense the integrity of the Golgi complex. (biologists.org)
  • acetylation
  • Importantly, loss of MLK4 function led to delayed flowering by decreasing phosphorylation of H2A serine 95, along with attenuated accumulation of H2A.Z and the acetylation of H4 at GI , thus reducing GI expression. (plantcell.org)
  • vitro
  • Figure S2 In vitro phosphorylation sites in TACC3 determined by mass spectrometry. (rupress.org)
  • Figure S4 Aurora A phosphorylation mutant of TACC3 forms a complex with XMAP215 in X. laevis egg extracts and in vitro. (rupress.org)
  • Coomassie staining), mitochondrial membrane potential (by JC-1), DNA damage (comet assay), oxidative damage (malondialdehyde (MDA) production), in vitro fertilization and protein phosphorylation (immunodetection), and erythrocyte acetylcholinesterase (AChE) activity. (osti.gov)
  • It has been shown that CHK1 can phosphorylate Wee 1 in vitro , providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by CHK1 over-expression, is due to phosphorylation by CHK1. (acris-antibodies.com)
  • cyclin
  • Specific cyclin-cdk complexes affect transcription through phosphorylation of components of the basal transcription apparatus (RNA polymerases and basal transcription factors), transcriptional modulators such as the retinoblastoma protein Rb, and transcription factors. (embopress.org)
  • antibody
  • The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. (abcam.com)
  • Phosphorylation site-specific antibody selective for the phosphorylated form of human tau containing a phosphate on serine 409. (abcam.cn)
  • The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. (abcam.cn)
  • cell
  • Here, we examine the function of H3 phosphorylation in cell transformation in vivo . (aacrjournals.org)
  • These results indicate that the phosphorylation of histone H3 at Ser 10 is an essential regulatory mechanism for EGF-induced neoplastic cell transformation. (aacrjournals.org)
  • ARHGAP19 is a hematopoietic-specific RhoGAP that acts through the RhoA/ROCK pathway to critically regulate cell elongation and cytokinesis during lymphocyte mitosis. (biologists.org)
  • Disruption of these phosphorylation sites results in premature localization of ARHGAP19 at the cell membrane and in its enrichment to the equatorial cortex in anaphase leading to cytokinesis failure and cell multinucleation. (biologists.org)
  • The activity of APC/C is tightly controlled: It is active from late mitosis until the end of G1 of the next cell cycle. (pnas.org)
  • physiological
  • However, TFOs with 2'-AE residues were more stable in physiological pH than those with only 2'-OMe sugars, as a simple function of 2'-AE content. (jove.com)