MCM is loaded onto DNA, facilitated by the loading protein Cdt1 (6), at origins of replication by the origin recognition complex (ORC) during late mitosis and G1 phase, becoming activated during S-phase to drive DNA replication. (biologists.com)
Cdt16
Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
The pre-replication complex (pre-RC) assembly or the DNA replication licensing is the first step in DNA replication initiation, characterized by the sequential recruitment of ORCs, Cdc6, Cdt1 and MCMs to the DNA replication origins to form the pre-RC at the end of mitosis ( Bell and Dutta 2002 ). (intechopen.com)
Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. (prospecbio.com)
MCM loss' zygotes were generated by preventing the factor geminin from being degraded as the presence of geminin stops Cdt1 directed loading of MCM during G1 phase (6). (biologists.com)
Anti-human Geminin mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Geminin amino acids 1-209 purified from E. coli. (prospecbio.com)
Geminin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. (prospecbio.com)
Metaphase-anaphase2
Geminin levels drop at the metaphase-anaphase transition of mitosis when it is degraded by the anaphase-promoting complex. (wikipedia.org)
Securin is involved in the control of the metaphase-anaphase transition during MITOSIS . (nih.gov)
Ubiquitination1
HN - 2014 FX - Ammonia MH - Anaphase-Promoting Complex-Cyclosome UI - D064173 MN - D8.811.464.938.750.92 MN - D12.776.167.24 MS - An E3 ubiquitin ligase primarily involved in regulation of the metaphase-to-anaphase transition during MITOSIS through ubiquitination of specific CELL CYCLE PROTEINS. (nih.gov)
Transition3
Herein, we identified a novel transcription factor in silk glands, ZFP67, which is gradually reduced in silk glands during the transition of mitosis to endomitosis. (bvsalud.org)
These data reveled that ZFP67 played an important role in transition of mitosis to endomitosis. (bvsalud.org)
In conclusion, it can be speculated that the decreasing expression of ZFP67 in silk glands during the transition stage of mitosis-to-endomitosis resulted in the lack of cyclin B, which further led to unsuccessful cytokinesis and then promoted the transition from mitosis to endomitosis of silk gland cells. (bvsalud.org)
Inhibition1
When the interaction between geminin and SWI/SNF is eliminated, geminin's inhibition to this process is eliminated and neural precursors are allowed to differentiate. (wikipedia.org)
Chromatin3
Geminin promotes early neural fate commitment by hyperacetylating chromatin. (wikipedia.org)
Geminin has also been shown to interact with the SWI/SNF chromatin remodeling complex. (wikipedia.org)
Fly Embryos: ORC subunits 2 to 6 (grey cylinders) remain bound to chromatin throughout the cell cycle, but Orc1 (red cylinder) is selectively ubiquitinated during mitosis by the APC/Fzr system and degraded. (nih.gov)
Thus, hamster ORC activity was absent during mitosis and early G1 phase, and reappeared as cells progressed through G1 phase. (nih.gov)
Substrate1
Consequently, cells with depleted USP7 accumulate Aurora-A kinase, a CHFR substrate, thus elevating multipolar mitoses. (nih.gov)
Protein4
A nuclear protein present in most eukaryotes and highly conserved across species, numerous functions have been elucidated for geminin including roles in metazoan cell cycle, cellular proliferation, cell lineage commitment, and neural differentiation. (wikipedia.org)
Geminin is a nuclear protein made up of about 200 amino acids, with a molecular weight of approximately 25 kDa. (wikipedia.org)
In this study, we identified a novel function of ubiquitin (Ub)-specific processing protease-7 (USP7) that interacts and cooperates with protein death domain-associated protein (Daxx) in the regulation of mitosis and taxane resistance. (nih.gov)
12. Promotion of mitosis by activated protein kinase B after DNA damage involves polo-like kinase 1 and checkpoint protein CHFR. (nih.gov)
Regulate1
Thus, USP7 and Daxx are necessary to regulate proper execution of mitosis, partially via regulation of CHFR and Aurora-A kinase stability. (nih.gov)
Nuclear2
GFP-E2F1 1-230 (green) is nuclear during G1 and G2 and fills the cell during mitosis. (elifesciences.org)
The net result is that the assembly of pre-RCs at specific sites is delayed until mitosis is completed and a nuclear membrane is assembled (1). (nih.gov)
Regulation1
20. Regulation of Polo-like kinase 1 by DNA damage in mitosis. (nih.gov)
Stability1
Geminin has been found to be overexpressed in several malignancies and cancer cell lines, while there is data demonstrating that geminin acts as a tumor suppressor by safeguarding genome stability. (wikipedia.org)
Cells4
Ultimately, geminin allows cells uncommitted to any particular lineage to acquire neural characteristics. (wikipedia.org)
In neural precursor cells, high levels of geminin prevent terminal differentiation. (wikipedia.org)
Cells progress directly from G2 into G1 without an intervening mitosis, resulting in an additional S-phase. (elifesciences.org)
Zhu W, Depamphilis ML. Selective killing of cancer cells by suppression of geminin activity . (nih.gov)
Cell4
In these cell lines, geminin knockdown leads to markedly slowed growth and apoptosis within several days. (wikipedia.org)
Since geminin knockdown leads to cell death in many cancer cell lines but not primary cell lines, it has been proposed as a potential therapeutic target for cancer treatment. (wikipedia.org)
RFP-CycB 1-266 (magenta) is cytoplasmic during S-phase and G2 and fills the cell during mitosis. (elifesciences.org)
Molecular interactions during mitosis prevent licensing of DNA to replicate prior to cell division. (nih.gov)
Function2
Model organisms have been used in the study of geminin function. (wikipedia.org)