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MitosisCyclinsCyclin BCell CycleCyclin-Dependent KinasesCell Cycle ProteinsCDC2 Protein KinaseCyclin D3Spindle ApparatusCyclin AG1 PhaseCyclin D2Cyclin DCyclin B1Cyclin EAnaphaseS PhaseInterphaseCyclin A2Cyclin GG2 PhaseCyclin D1MetaphaseUbiquitin-Protein Ligase ComplexesAnaphase-Promoting Complex-CyclosomeProtein-Serine-Threonine KinasesChromosome SegregationCyclin-Dependent Kinase 4CentrosomeMicrotubulesCyclin-Dependent Kinase 2CDC28 Protein Kinase, S cerevisiaeHeLa CellsTelophasePhosphorylationKinetochoresCDC2-CDC28 KinasesCell DivisionChromosomesSaccharomyces cerevisiae ProteinsRetinoblastoma ProteinCdh1 ProteinsNocodazoleCyclin-Dependent Kinase Inhibitor ProteinsCyclin G1Molecular Sequence DataNuclear ProteinsCyclin A1Microtubule-Associated ProteinsFungal ProteinsCell Nucleus DivisionCdc20 ProteinsCell NucleusAurora KinasesSchizosaccharomycesCyclin-Dependent Kinase Inhibitor p27Amino Acid SequenceSaccharomyces cerevisiaeCyclin-Dependent Kinase 6cdc25 PhosphatasesMeiosisSchizosaccharomyces pombe ProteinsCyclin B2Protein KinasesDNA ReplicationCytokinesisMutationAurora Kinase BCentromereProtamine KinaseCell LineChromatidsChromosomal Proteins, Non-HistoneGenes, cdcMitosis ModulatorsProphaseSaccharomycetalesXenopus ProteinsOncogene ProteinsProtein BindingXenopusApc3 Subunit, Anaphase-Promoting Complex-CyclosomeDrosophila ProteinsMicroscopy, FluorescenceTubulinRecombinant Fusion ProteinsNuclear EnvelopeKinesinCell Cycle CheckpointsApc8 Subunit, Anaphase-Promoting Complex-CyclosomeCyclin-Dependent Kinase Inhibitor p21Mad2 ProteinsBase SequenceCell ProliferationGene Expression Regulation, FungalMitotic IndexModels, BiologicalLigasesDNA-Binding ProteinsMaturation-Promoting Factor
Cdk1KinasesCdc2CytokinesisCDK2CDKsChromosomesRegulatesCCNB1Metaphase-to-anaphase transitionDispensable for mitosisINTERPHASEDegradation of cyclinsAnaphaseProteinsPhosphorylation by cyclinCellsProteinTransitionEukaryotesMaturation-promotNuclearInducesSpindle assemblyKinase inhibitorBiolMicrotubulesComplexesChromatinDependentCheckpointPathwaySubunitActivitySynthesisRegulationPhaseAccumulation2000InactivationCognateGeneCytoplasmicMeiosisInfrequentNucleus
Cdk136
  • Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated. (wikipedia.org)
  • Cyclin B1-Cdk1 is involved in the early events of mitosis, such as chromosome condensation, nuclear envelope breakdown, and spindle pole assembly. (wikipedia.org)
  • Once activated, cyclin B1-Cdk1 promotes several of the events of early mitosis. (wikipedia.org)
  • Another important function of the cyclin B1-Cdk1 complex is to break down the nuclear envelope. (wikipedia.org)
  • Phosphorylation of the lamins by cyclin B1-Cdk1 causes them to dissociate, compromising the structural integrity of the nuclear envelope so that it breaks down. (wikipedia.org)
  • Just prior to mitosis, a large amount of cyclin B1 is present in the cell, but it is inactive due to phosphorylation of Cdk1 by the Wee1 kinase. (wikipedia.org)
  • Once cyclin B1-Cdk1 is activated, it remains stably active for the rest of mitosis. (wikipedia.org)
  • Another mechanism by which cyclin B1-Cdk1 activity is regulated is through subcellular localization. (wikipedia.org)
  • This is regulated by the phosphorylation of cyclin B1, in contrast to phosphorylation of Cdk1 regulating the activity of the complex. (wikipedia.org)
  • Cyclin B1 is phosphorylated by Polo kinase and Cdk1, again setting up a positive feedback loop that commits cyclin B1-Cdk1 to its fate. (wikipedia.org)
  • Cyclin B1 has been shown to interact with Cdk1, GADD45A and RALBP1. (wikipedia.org)
  • The final barrier to mitotic establishment corresponds to nuclear envelope breakdown, which requires a decisive shift in the balance of cyclin-dependent kinase Cdk1 and PP2AB55 activity. (bvsalud.org)
  • Beyond this point, cyclin B /Cdk1 is essential for phosphorylation of a distinct subset of mitotic Cdk1 substrates that are essential to complete cell division . (bvsalud.org)
  • Our results identify how cyclin A , cyclin B and Greatwall kinase coordinate mitotic progression by increasing levels of Cdk1-dependent substrate phosphorylation . (bvsalud.org)
  • 15. Targeting cyclin-dependent kinase 1 (CDK1) but not CDK4/6 or CDK2 is selectively lethal to MYC-dependent human breast cancer cells. (nih.gov)
  • controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. (proteopedia.org)
  • ORC is phosphorylated by Cdk1/cyclin A during G2/M and released from chromatin. (nih.gov)
  • Xic1, a Cdk specific inhibitor, is present prior to S-phase, and presumably inhibits the ORC associated Cdk1/cyclin A activity, thereby allowing ORC to bind to chromatin. (nih.gov)
  • In mitotic cells, Orc1 is hyperphosphorylated by its association with Cdk1/cyclin A, then dephosphorylated and bound to chromatin during the M to G1-phase transition (10). (nih.gov)
  • 1999). Cyclin B-cdk1 kinase stimulates ORC- and Cdc6-independent steps of semiconservative plasmid replication in yeast nuclear extracts. (sdbonline.org)
  • The model was built by integrating and modifying two previously published models such that the model outputs for cyclins A and B fit cyclin expression measurements and the activation of B cyclin/Cdk1 coincided with phosphorylation of histone H3. (ebi.ac.uk)
  • The model depends on Cdh1-regulated cyclin degradation during G1, regulation of B cyclin/Cdk1 activity by cyclin A/Cdk via Wee1, and transcriptional control of the mitotic cyclins that reflects some of the current literature. (ebi.ac.uk)
  • Cdk1 activity was also required for keeping NPCs and ALPCs disassembled during mitosis. (diva-portal.org)
  • In Agreement recombinant Cdk1/cyclin B was able to induce phosphorylation and dissociation of nucleoporins from the NPCs in vitro. (diva-portal.org)
  • These genes include cell cycle regulatory cyclins and cyclin-dependent proteins CCNA2, CCNB1 and CDK1. (researchsquare.com)
  • It was found that vitexicarpin may block the cell cycle in Growth 2 Phase (G2)/Mitosis (M) phase by inhibiting the expression of Cyclin-Dependent Kinase 1 (CDK1), Cellular Myelocytomatosis Oncogene (c-myc) and survivin, thereby inhibiting the proliferation and promoting the apoptosis of human non-small cell lung cancer cell line (H322) [ 3 ]. (ijpsonline.com)
  • This adaptation pathway turns off the Cdk1/cyclin B complex, whose activity defines mitosis. (rupress.org)
  • A similar abrupt change was expected in vertebrate cells, via Cdk1 phosphorylation, induction of a Cdk1 inhibitor, or rapid degradation of cyclin B. "But it turns out it doesn't do any of these," says Rieder. (rupress.org)
  • These variations on the theme of DNA replication and division have been studied in strains of fission yeast, Schizosaccharomyces pombe , carrying mutations that interfere with the function of mitotic cyclin-dependent kinase (Cdk1:Cdc13) without impeding the roles of DNA-replication loading-factor (Cdc18) and S-phase cyclin-dependent kinase (Cdk1:Cig2). (cellcycle.org.uk)
  • Present study was aimed to find out whether RO-3306, specific cyclin dependent kinase 1 (Cdk1) inhibitor could protect against postovulatory aging-mediated spontaneous exit from M-II arrest in rat eggs cultured in vitro. (cgrpreceptor.com)
  • The morphological changes, percentage of spontaneous exit from M-II arrest, total and specific phosphorylation status of Cdk1, cyclin B1 level and Cdk1 activity were analyzed. (cgrpreceptor.com)
  • Postovulatory aging increased Thr14/Tyr15 phosphorylated Cdk1 level, decreased Thr161 phosphorylated Cdk1 as well as cyclin B1 levels and increased Cdk1 activity in aged eggs cultured in vitro. (cgrpreceptor.com)
  • On the other hand, RO-3306 protected postovulatory aging-induced changes in specific phosphorylation of Cdk1, cyclin B1 level, inhibited the kinase activity and prevented spontaneous exit from M-II arrest. (cgrpreceptor.com)
  • Conclusions: Our results suggest that postovulatory aging destabilizes MPF by modulating specific phosphorylation of Cdk1 and cyclin B1 level. (cgrpreceptor.com)
  • It is a heterodimer protein containing cyclin dependent kinase 1 (Cdk1) as a catalytic subunit and cyclin B1 as a regulatory subunit [1,5,6]. (cgrpreceptor.com)
  • MPF stabilization requires association of cyclin B1 with Cdk1 [3,5,7], while dissociation of cyclin B1 from MPF heterodimer and its degradation destabilizes MPF and triggers meiotic resumption from diplotene as well as metaphase-II (M-II) arrest [3-5,7-13]. (cgrpreceptor.com)
Kinases13
Cdc210
  • For example, an increase in expression of cyclin B1/cdc2 is significantly higher in breast tumor tissue and shown to increase lymph node metastasis in breast cancer. (wikipedia.org)
  • Herein, we report that the anti-apoptotic protein, Bcl-2, undergoes cell cycle-dependent phosphorylation during mitosis when there is elevated Cdc2 activity. (nih.gov)
  • However, the kinase was likely not cyclin B1/Cdc2, since cyclin B1/Cdc2 was not detectable in Bcl-2 immunoprecipitates, nor was recombinant Bcl-2 phosphorylated in vitro by cyclin B1/Cdc2. (nih.gov)
  • Mitosis is directly linked to RHAMM by suppressing cell cycle-dependent kinase (Cdc2) and cyclin B1 expression. (medscape.com)
  • Cyclin-dependent kinase (CDK Cdc2 ) is the master regulator of mitosis and cell division, and therefore the propensity for smaller cells not to divide must ultimately feed into the regulation of CDK activity ( Coudreuse and Nurse, 2010 ). (elifesciences.org)
  • 1997). Hyperphosphorylation of the N-terminal domain of Cdc25 regulates activity toward cyclin B1/cdc2 but not cyclin A/cdk2. (sdbonline.org)
  • Cdk2 kinase is required for entry into mitosis as a positive regulator of Cdc2-cyclin B kinase activity. (sdbonline.org)
  • Flavopiridol treatment of docetaxel-treated cells enhances the exit from mitosis with a more rapid decrease in mitotic markers including MPM-2 labeling and cyclin B1/cdc2 kinase activity. (aacrjournals.org)
  • Furthermore, this intestinal clock controls the expression of key cell cycle regulators, such as cdc2, wee1, p21, PCNA and cdk2, but only weakly influences cyclin B1, cyclin B2 and cyclin E1 expression. (ucl.ac.uk)
  • Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. (nih.gov)
Cytokinesis6
CDK28
  • 10. Structure of cyclin-dependent kinase 2 (CDK2) in complex with the specific and potent inhibitor CVT-313. (nih.gov)
  • Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. (proteopedia.org)
  • NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. (proteopedia.org)
  • Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. (proteopedia.org)
  • Zhao J, Kennedy BK, Lawrence BD, Barbie DA, Matera AG, Fletcher JA, Harlow E. NPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription. (proteopedia.org)
  • Cell cycle-regulated phosphorylation of p220(NPAT) by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription. (proteopedia.org)
  • In yeast, for instance, cell growth induces accumulation of Cln3 cyclin , which complexes with the cyclin dependent kinase CDK2. (wikipedia.org)
  • During early S-phase, the cyclin E-Cdk2 complex phosphorylates NPAT , a nuclear coactivator of histone transcription. (wikipedia.org)
CDKs2
  • The role of cyclin B1 is to transition the cell from G2 to M phase but becomes unregulated in cancer cells where overexpression of cyclin B1 can lead to uncontrolled cell growth by binding to its partner Cdks. (wikipedia.org)
  • IMPORTANCE Giardia lamblia CDKs (GlCDKs) and their cognate cyclins have not yet been studied. (gene-tools.com)
Chromosomes4
Regulates8
  • 14. Cyclin-dependent kinase 9 activity regulates neutrophil spontaneous apoptosis. (nih.gov)
  • regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. (proteopedia.org)
  • 1999). p53 regulates a G2 checkpoint through cyclin B1. (sdbonline.org)
  • 2000). Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction. (sdbonline.org)
  • In eukaryotes, the anaphase-promoting complex (APC/C, also known as the cyclosome) regulates the ubiquitin-dependent proteolysis of specific cell-cycle proteins to coordinate chromosome segregation in mitosis and entry into the G1 phase. (rcsb.org)
  • Here we show that the zebrafish gut contains a directly light-entrainable circadian pacemaker, which regulates the daily timing of mitosis. (ucl.ac.uk)
  • promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. (nih.gov)
  • The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. (nih.gov)
CCNB11
  • G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. (wikipedia.org)
Metaphase-to-anaphase transition3
  • In this study, we report the use of time-lapse microscopy and a human kinesin endoribonucleases RNase III-prepared short interfering RNA (esiRNA) library to identify Kif4 as a motor protein that translocates PRC1, a spindle midzone-associated cyclin-dependent kinase substrate protein, to the plus ends of interdigitating spindle microtubules during the metaphase-to-anaphase transition. (nih.gov)
  • HN - 2014 FX - Ammonia MH - Anaphase-Promoting Complex-Cyclosome UI - D064173 MN - D8.811.464.938.750.92 MN - D12.776.167.24 MS - An E3 ubiquitin ligase primarily involved in regulation of the metaphase-to-anaphase transition during MITOSIS through ubiquitination of specific CELL CYCLE PROTEINS. (nih.gov)
  • Degradation of these proteins controls processes such as entry of mitosis, the metaphase to anaphase transition and exit from mitosis and is thereby responsible for initiating the transition to the next phase of the cell cycle. (rug.nl)
Dispensable for mitosis2
INTERPHASE1
Degradation of cyclins2
Anaphase2
  • In cell cycle, the exit through the mitotic state is triggered from the release from the phosphatase Cdc14 through the anaphase stage of mitosis from nucleolar chromatin, where it really is held and sequestered inactive. (bioinf.org)
  • After anaphase, the high mitotic cyclin-dependent kinase (Cdk) activity is downregulated to promote exit from mitosis. (nih.gov)
Proteins6
Phosphorylation by cyclin1
Cells20
  • Cyclin E expression suppresses p53-dependent senescence after mitotic bypass, allowing cells to complete endoreduplication. (nih.gov)
  • activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. (proteopedia.org)
  • Using a model cell line in which p53 expression is regulated exogenously in a tetracycline-off system (TR9-7 cells), our laboratory has shown that arsenite disrupts mitosis and that p53-deficient cells [p53 (−) ], in contrast to p53-expressing cells [p53 (+) ], display greater sensitivity to arsenite-induced mitotic arrest and apoptosis. (nih.gov)
  • Arsenite-treated p53 (+) cells exited mitosis normally, whereas p53 (−) cells exited mitosis with delayed kinetics. (nih.gov)
  • MITOSIS is the division of the nucleus of body (somatic) cells into genetically identical daughter cells. (angelfire.com)
  • Cell size homeostasis is brought about by the co-ordination of cell division with cell growth and requires restriction of smaller cells from undergoing mitosis and cell division, whilst allowing larger cells to do so. (elifesciences.org)
  • CDK inhibitory phosphorylation works synergistically with PP2A to prevent mitosis in smaller cells. (elifesciences.org)
  • Finally, we find that diploid cells of equivalent size to haploid cells exhibit lower CDK activity in response to equal cyclin-CDK enzyme concentrations, suggesting that CDK activity is reduced by increased DNA levels. (elifesciences.org)
  • Thus, hamster ORC activity was absent during mitosis and early G1 phase, and reappeared as cells progressed through G1 phase. (nih.gov)
  • The disappearance of cyclin B at the end of mitosis is regulated spatially in Drosophila cells. (sdbonline.org)
  • Prophase - Prophase is the first step of mitosis, a type of cell division that produces two daughter cells with identical genomes. (biolegend.com)
  • In these studies we show that flavopiridol, a cyclin-dependent kinase inhibitor, potentiates docetaxel-induced apoptosis 3-fold in MKN-74 human gastric cells. (aacrjournals.org)
  • In contrast, pretreatment with flavopiridol prevents cells from entering mitosis by inhibiting cyclin B1/cdc-2 kinase activity, thus antagonizing the docetaxel effect. (aacrjournals.org)
  • Even when mitotic cells lack a spindle, cyclin (bottom panels) slowly fades away. (rupress.org)
  • Cells with a defective spindle get delayed-stuck in a mitotic checkpoint-but eventually escape mitosis. (rupress.org)
  • Instead, "human cells slowly degrade cyclin B over the course of time. (rupress.org)
  • This slow, proteasome-dependent degradation of cyclin B was required for nocodazole-treated cells to escape from mitosis. (rupress.org)
  • These cells have irregular and indented nuclei , moderately coarse chromatin, and meager cytoplasm, which are reminiscent of smaller cells of follicular lymphoma, but mitoses are more numerous and large cells are infrequent. (medscape.com)
  • Required in higher cells for entry into S-phase and mitosis. (nih.gov)
  • cells preparing for mitosis can reverse their decision to divide . (nih.gov)
Protein3
Transition5
Eukaryotes3
  • Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes , but their specialised functions in mitosis are incompletely understood. (bvsalud.org)
  • Disassembly and reassembly of the nuclear pore complexes (NPCs) is one of the major events during open mitosis in higher eukaryotes. (diva-portal.org)
  • In higher eukaryotes NPCs reversibly disassemble during mitosis into distinct nucleoporin subcomplexes. (diva-portal.org)
Maturation-promot1
Nuclear5
  • Phosphorylation of cyclin B1 causes it to be imported to the nucleus, and phosphorylation also prevents export from the nucleus by blocking the nuclear export signal. (wikipedia.org)
  • Nuclear-dominant expression of cyclin B1 leads to poorer prognosis due to its weak activity compared to cytoplasmic cyclin B1. (wikipedia.org)
  • M (Mitosis) PHASE is the period of nuclear division. (angelfire.com)
  • The net result is that the assembly of pre-RCs at specific sites is delayed until mitosis is completed and a nuclear membrane is assembled (1). (nih.gov)
  • En la mitosis, interiene en la transición G2/M. Una vez activado por las CICLINAS, el MPF fosforila directamente algunas de las proteínas que intervienen en la ruptura del envoltorio nuclear, en la condensación cromosómica, formación del huso y degración de las ciclinas. (bvsalud.org)
Induces1
  • [3] Active cyclin D-CDK4/6 complex induces release of E2F transcription factor, which in turn initiates expression of S-phase genes. (wikipedia.org)
Spindle assembly1
  • Summary: Phosphorylation of importin-α1 by cyclin - dependent kinase 1 provides spatial and temporal control of mitotic spindle assembly through the release of spindle-assembly factors from inhibitory complexes during mitosis. (biologists.com)
Kinase inhibitor1
  • 16. In vitro antitumor properties of a novel cyclin-dependent kinase inhibitor, P276-00. (nih.gov)
Biol1
  • The C-terminal regulatory domain of p53 contains a functional docking site for cyclin A. J Mol Biol. (proteopedia.org)
Microtubules2
Complexes2
  • [3] Mitogenic signals received throughout G1-phase cause gradual accumulation of cyclin D, which complexes with CDK4/6. (wikipedia.org)
  • Both pathways result in the inhibition of Cyclin-Cdk complexes and thereby induce cell cycle arrest. (rug.nl)
Chromatin2
  • Fly Embryos: ORC subunits 2 to 6 (grey cylinders) remain bound to chromatin throughout the cell cycle, but Orc1 (red cylinder) is selectively ubiquitinated during mitosis by the APC/Fzr system and degraded. (nih.gov)
  • Orc1 and Mcm3 were easily eluted from chromatin during mitosis and early G1 phase, but became stably bound during mid-G1 phase, concomitant with the appearance of the origin decision point (i.e. assembly of a functional pre-replication complex at ori -b). (nih.gov)
Dependent10
  • Here, we show that elevated cyclin E1 expression causes replicative stress, which activates ATR- and Chk1-dependent G2 phase arrest. (nih.gov)
  • p53, via its downstream target p21, together with Wee1, then inhibits mitotic cyclin-dependent kinase activity sufficiently to activate APC/C Cdh1 and promote mitotic bypass. (nih.gov)
  • 6. A novel series of highly potent 2,6,9-trisubstituted purine cyclin-dependent kinase inhibitors. (nih.gov)
  • 12. Characterization of molecular and cellular functions of the cyclin-dependent kinase CDK9 using a novel specific inhibitor. (nih.gov)
  • Therefore, scaling of cyclin-CDK levels with cell size, CDK inhibitory phosphorylation, PP2A, and DNA-dependent inhibition of CDK activity, all inform the cell cycle network of cell size, thus contributing to cell size homeostasis. (elifesciences.org)
  • Rum1 and Cdc18 link inhibition of cyclin-dependent kinase to the initiation of DNA replication in Schizosaccharomyces pombe . (sdbonline.org)
  • 1999). Cyclin-dependent kinase and Cks/Suc1 interact with the proteasome in yeast to control proteolysis of M-phase targets. (sdbonline.org)
  • Furthermore, we show that cyclin-dependent kinase phosphorylation of PRC1 controls the timing of PRC1 translocation by Kif4. (nih.gov)
  • thus cyclin - dependent kinase (CDK) must actively contribute to stationary phase establishment. (biologists.com)
  • Mark C. Hall ABSTRACT Inactivation of cyclin - dependent kinase (Cdk) and reversal of Cdk phosphorylation are universally required for mitotic exit. (biologists.com)
Checkpoint1
Pathway2
  • Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. (bvsalud.org)
  • In wild-type (WT) p53 tumors, WGD is frequently associated with activation of the E2F pathway, especially amplification of CCNE1, encoding cyclin E1. (nih.gov)
Subunit1
  • Efficient phosphorylation of the auto-inhibitory segment, and thus relief of auto-inhibition, requires the recruitment of Cdk-cyclin in complex with a Cdk regulatory subunit (Cks) to a hyperphosphorylated loop of Apc3. (rcsb.org)
Activity4
  • It is likely that potential size control pathways will be integrated at the level of CDK activity control because CDK activity is the driver of mitosis. (elifesciences.org)
  • Using a novel CDK activity sensor, we have monitored cell size, CDK activity, and cyclin-CDK complex level simultaneously, whilst genetically varying regulators of the cyclin-CDK control system. (elifesciences.org)
  • 1995). Reversible phosphorylation controls the activity of cyclosome-associated cyclin-ubiquitin ligase. (sdbonline.org)
  • CDK activity rises over 50-fold during the cell cycle, from a low level in G1 to a high level in mitosis. (biologists.com)
Synthesis1
  • We introduced autocatalytic transcription of E2F, E2F regulated transcription of cyclin B, Cdc20/Cdh1 mediated E2F degradation, enhanced transcription of mitotic cyclins during late S/early G2 phase, and the sustained synthesis of cyclin B during mitosis. (ebi.ac.uk)
Regulation2
  • While the exact mechanism that explains how cyclin B1 becomes overexpressed is not very well understood, previous work has shown that down regulation of cyclin B1 can lead to tumor regression. (wikipedia.org)
  • Here, therefore, we have studied in vivo regulation of cyclin-CDK activation at mitosis in the fission yeast. (elifesciences.org)
Phase2
Accumulation1
20001
Inactivation1
Cognate1
  • Coimmunoprecipitation experiments with GlCDKs and the nine putative G. lamblia cyclins (Glcyclins) identified Glcyclins 3977/14488/17505 and 22394/6584 as cognate partners of GlCDK1 and GlCDK2, respectively. (gene-tools.com)
Gene1
Cytoplasmic1
Meiosis1
  • En la meiosis, induce a los ovocitos inmaduros a emprender la maduración meiótica. (bvsalud.org)
Infrequent1
Nucleus2