• Two of the phosphotyrosine-containing proteins, migrating in SDS-polyacrylamide gels of approximately 43 and 45 kDa, corresponded in mobility to phosphorylated species of the 42- and 44-kDa mitogen-activated protein kinase (MAPK) isoforms. (nih.gov)
  • Chromatography of soluble fractions from these cells on Mono Q columns revealed early and late eluting peaks of myelin basic protein kinase activity, which contained the 42- and 44-kDa MAPK isoforms, respectively. (nih.gov)
  • Further studies into the mechanisms by which MAPK was activated by 20:4(n-6) strongly suggested the involvement of protein kinase C (PKC). (nih.gov)
  • Not only did incubation of WB cells with 20:4(n-6) result in the translocation of PKC alpha, delta, and epsilon to a particulate fraction, it was found that the fatty acid failed to activate MAPK in cells pretreated for 26 h with phorbol 12-myristate 13-acetate, which depleted WB cells of PKC alpha, delta and epsilon. (nih.gov)
  • In addition, fatty acids of the n-3 series were effective activators of MAPK. (nih.gov)
  • 예를 들어, 제 3형 분비 시스템에서 유래한 다양한 단백질들은 MAPK(mitogen activated protein kinase) 경로를 간섭한다. (natureasia.com)
  • In the present study, we investigated whether contractions of isolated muscles induced phosphorylation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) and p38 MAPK in a fibre-type dependent manner. (elsevierpure.com)
  • The total amount of ERK1/2 and p38 MAPK proteins was higher in the slow-twitch soleus muscle. (elsevierpure.com)
  • The disorder is caused by a dominantly inherited FGFR3 mutation that permanently activates the fibroblast growth factor receptor 3 (FGFR3) and its downstream mitogen-activated protein kinase (MAPK) signaling pathway. (medscape.com)
  • G protein-coupled receptors can activate Erk MAPK pathways through a variety of mechanisms, including stimulation of Src, phosphoinositide-3 kinase (PI-3-K), protein kinase C (PKC), or the epidermal growth factor (EGF) receptor tyrosine kinase. (aspetjournals.org)
  • In isolated strips of rat aorta, the MAPK/Erk kinase inhibitor U0126 (50 μM), Src inhibitor PP1 (0.5 μM), PKC inhibitors calphostin C (1 μM) and chelerythrine (10 μM), and the PI-3-K inhibitor LY294002 (1-20 μM) reduced 5-HT-induced contraction. (aspetjournals.org)
  • Effect of p38 mitogen-activated protein kinases (MAPK) inhibitor on nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activity. (molvis.org)
  • Here, we review the applications of LIPUS against inflammation through different signaling pathways including nuclear factor-κB (NF-κB), mitogen-activated protein kinase (MAPK), and phosphatidylinositol-3-kinase/serine/threonine kinase (PI3K/Akt), and discuss the underlying mechanisms. (qigonginstitute.org)
  • OBJECTIVE: To study the effects of benzo(a)pyrene (BaP) on the cell cycle distribution and activities of mitogen-activated protein kinase (MAPK) signal molecules (ERK1/2, JNK1/2 and p38) in human embryo lung cells (HELF), and to investigate the relationship between alterations of MAPK protein phosphorylation and the cell cycle distributions. (uky.edu)
  • METHODS: The phosphorylation of MAPK were induced by exposing HELF cells to BaP at 0.1, 0.5, 2.5 and 12.5 micromol/L. The phosphorylation and protein expression levels of ERK1/2, JNK1/2 and p38 were determined through western-blotting assay. (uky.edu)
  • leads to modifications in mitogen turned on proteins kinase (MAPK) signaling pathways as the 106-126 peptide activates development aspect related signaling pathways including vascular endothelial development aspect (VEGF) signaling as well as the phosphoinositide-3 kinase (PI3K) pathway. (fabretp.org)
  • The signal transduction pathways for AP-1 activation were also investigated and the results demonstrate that TiO2 stimulates mitogen-activated protein kinase (MAPK) family members, including extracellular signal-regulated protein kinases (ERKs), p38 kinase, and C-Jun N-terminal kinase (JNKs). (cdc.gov)
  • Subsequently, the phosphatidylinositol-3-kinase and mitogen-activated protein kinase pathways can be activated, resulting in proliferation and inhibition of apoptosis ( 13 , 14 ). (snmjournals.org)
  • MAPK3, also known as ERK1 (Extracellular Signal-Regulated Kinase 1), is a serine/threonine kinase involved in cell signaling pathways. (creativebiomart.net)
  • Activation of mitogen-activated protein (MAP) kinases has been implicated in the signal transduction pathways linking exercise to adaptive changes of muscle protein expression. (elsevierpure.com)
  • In conclusion, MAP kinase signalling pathways are differentially activated and expressed in slow- and fast-twitch muscles. (elsevierpure.com)
  • However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. (origene.com)
  • Pathway evaluation of kinome data The kinome data was put through pathway analysis to determine which cellular pathways/processes are activated beneath the different treatment conditions. (fabretp.org)
  • These findings demonstrate that TiO2 nanoparticle s stimulated the generation of ·OH radicals and induces AP-1 activation, which may be mediated through p38 kinase and ERKs pathways. (cdc.gov)
  • Phosphorylation of extracellular signal-regulated kinase-1 (ERK-1), ERK-2, and p38 MAP kinase by LPS were demonstrated on Western blot analysis. (nih.gov)
  • 5-Hydroxytryptamine (5-HT) activates the extracellular signal-regulated kinase (Erk) mitogen-activated protein kinases (MAPKs) in the vasculature, resulting in contraction. (aspetjournals.org)
  • Xiao, L, Haack, KKV & Zucker, IH 2013, ' Angiotensin II regulates ACE and ACE2 in neurons through p38 mitogen-activated protein kinase and extracellular signal-regulated kinase 1/2 signaling ', American Journal of Physiology - Cell Physiology , vol. 304, no. 11, pp. (nebraska.edu)
  • MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. (antibodies-online.com)
  • MK-8776 ERK1/2) pathway and phosphatidylinositide 3-kinases (PI3K) pathway [1C4]. (phytid.org)
  • This gene encodes a scaffold protein that is thought to be involved in the regulation of the c-Jun amino-terminal kinase signaling pathway. (nih.gov)
  • In vitro , GnRH neuronal cell lines respond to a variety of ligands that activate the Jak (Janus-activated kinase)/STAT (signal transducers and activators of transcription) intracellular signaling pathway. (jneurosci.org)
  • The ER is activated by estrogen binding and also by phosphorylation through the PI3K/Akt pathway ( 4 ). (iiarjournals.org)
  • During the transient loss of p38 activity there was a temporary Fas-induced increase in phosphatidylinositol 3-kinase (PI3K) activity, which also had a pro-apoptotic impact on the neutrophils. (lu.se)
  • This gene encodes a serine/threonine protein kinase that forms a component of protein kinase-mediated signal transduction cascades. (wikipedia.org)
  • In addition, my experiments showed that the active form of p38 associates with caspase 8 and caspase 3, which is necessary for p38-induced phosphorylation of serine-362 and serine-150 on these caspases. (lu.se)
  • The receptor, tyrosine kinase c-Ret, had been explored as an oncogene, which also has been mutated in patients with multiple endocrine neoplasia and familial medullary thyroid cancer syndromes. (hindawi.com)
  • On binding of insulinlike growth factors to the receptor, adaptor proteins, such as insulin-receptor substrate-1 (IRS-1) and IRS-2, are recruited to the phosphorylation site of the cytoplasmic domain ( 19 - 21 ). (snmjournals.org)
  • The phosphorylated receptor provides multiple docking sites that recruit STAT proteins, of which there are six family members (STAT1-6) ( Pellegrini and Dusanter-Fourt, 1997 ). (jneurosci.org)
  • CAAX peptidomimetic FTI-244 decreases platelet-derived growth factor receptor tyrosine phosphorylation levels and inhibits stimulation of phosphatidylinositol 3-kinase but not mitogen-activated protein kinase. (nyu.edu)
  • The EGF receptor tyrosine kinase inhibitor AG1478 (0.25-1 μM) was without effect. (aspetjournals.org)
  • This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. (origene.com)
  • Because the expression of the estrogen receptor (ER) in patients with primary breast cancer is one of the most powerful indicators of response to adjuvant hormonal therapy, the effects of endocrine-therapeutic agents strongly depend on whether tumor cell growth remains estrogen responsive ( 3 ). (iiarjournals.org)
  • There is also decreased mRNA and proteins great quantity from the transcription element, early development response 1 (Egr1) in inhibitor-treated granulosa cells. (phytid.org)
  • PD 098059 (10 microM), an inhibitor of upstream activator of MKK-1, inhibited GM-CSF expression, but the expression of GM-CSF was not inhibited by SB 203580 (10 microM), an inhibitor of p38-MAP kinase. (nih.gov)
  • Alternatively, pharmacologic approach to inhibition of the proteins activity is normally economical, less frustrating and not at all hard compared to hereditary manipulation. (phytid.org)
  • Another challenge lies in achieving selective inhibition of specific MAPK3 isoforms without affecting other closely related kinases. (creativebiomart.net)
  • Growth inhibition was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and apoptosis was assayed by ELISA. (aacrjournals.org)
  • 0.05) erlotinib-induced growth inhibition and apoptosis in BxPC-3, CAPAN-2, and AsPC-1 cells. (aacrjournals.org)
  • Mostajeran M, Wetterling F, Blixt FW, Edvinsson L, Ansar S. Acute mitogen-activated protein kinase 1/2 inhibition improves functional recovery and vascular changes after ischaemic stroke in rat-monitored by 9.4 T magnetic resonance imaging. (lu.se)
  • MAPK3 also phosphorylates other kinases, such as p90RSK, which further propagate the signal to regulate cellular processes like cell cycle progression, apoptosis, and differentiation. (creativebiomart.net)
  • These biochemical modifications impair the activities, and possibly also the stability, of caspase 8 and 3 and thereby weaken the capacity of these enzymes to induce apoptosis. (lu.se)
  • Activated drosophila ras1 is selectively suppressed by isoprenyl transferase inhibitors. (nyu.edu)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) in Tissue homogenates, cell lysates and other biological fluids. (ibiomagazine.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (ibiomagazine.org)
  • GM-CSF expression and release in AM is controlled by NF-kappaB activation, and this is modulated by phosphorylation of MKK-1 and p38 MAP kinase acting on histone acetylation. (nih.gov)
  • DOK5 enhances c-Ret-dependent activation of mitogen-activated protein kinase [ 3 ]. (hindawi.com)
  • The results showed that Tau treatment induced the decrease of TUNEL-positive cells, prohibited the disturbance of Bcl-2 and Bax expression, and inhibited Cyt C release and caspase-8 and caspase-3 activation significantly. (springer.com)
  • In the BxPC-3 cell line, significant down-regulation of EGFR, phosphorylated Akt, NF-κB activation, and survivin was observed in the cells treated with the combination compared with the erlotinib-treated cells. (aacrjournals.org)
  • Both isoform 3 of the human mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4) and Traf2- and Nck-interacting kinase (TNIK) are putative effectors of Rap2 in mediating the activation of c-Jun N-terminal kinase (JNK) to regulate the actin cytoskeleton. (nih.gov)
  • The activation of this kinase requires its phosphorylation by upstream kinases. (antibodies-online.com)
  • Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. (antibodies-online.com)
  • Titanium dioxide nanoparticle induced AP-1 activation via ERKs and p38 kinase. (cdc.gov)
  • We now report that incubation of WB cells with 20:4(n-6) resulted in the appearance of several tyrosine-phosphorylated cytosolic proteins. (nih.gov)
  • A signaling protein, i.e., a protein that circulates extracellularly influencing other cells to turn on the process of protein synthesis. (lupusresearch.org)
  • It activates transcription and contributes to proliferation, antiapoptosis, and metastasis of tumor cells ( 5 ). (iiarjournals.org)
  • CD95 counts in the 3 types of white blood cells were significantly higher in PEM infants and showed improvement after nutritional rehabilitation yet not reaching the control values. (who.int)
  • Since activator protein-1 (AP-1) is known to play an important role in the induction of neoplastic transformation and regulation of multiple genes involved in cell proliferation and inflammation, we investigate the potency of TiO2 nanoparticle s on reactive oxygen species (ROS) generation and AP-1 signaling in a mouse epithermal cell line, JB6 cells. (cdc.gov)
  • TiO2 nanoparticle s caused a 3-fold increase in AP-1 activity in the cells. (cdc.gov)
  • We determined the mechanisms of GM-CSF regulation in AM from normal volunteers activated by lipopolysaccharide (LPS) by examining the role of nuclear factor-kappaB (NF-kappaB), and of p38 mitogen-activated protein (MAP) kinase and MAP kinase kinase (MKK-1). (nih.gov)
  • Fibroblast growth factor homologous factors are intracellular signaling proteins. (nih.gov)
  • The cytokine receptors lack intrinsic kinase activity and require interaction with members of the Jak family of intracellular signaling molecules. (jneurosci.org)
  • 1995 Mar 3;270(9):4201-4. (nih.gov)
  • Journal of the American Chemical Society 1995 , 117(3), 1143-1144. (nyu.edu)
  • Drug News and Perspectives 1995 , 3, 138. (nyu.edu)
  • Detection and quantification of fecal influenza A viral RNA were performed by quantitative real-time reverse transcription PCR (RT-PCR) specific for the viral matrix-gene as described ( 1 - 3 ). (cdc.gov)
  • To test the specificity of the staining, negative controls using protein block reagent in replacement of the lectin, and isotype-matched control in replacement of the primary antibody were included. (cdc.gov)
  • Encodes a protein containing a domain with significant homology to the MACPF (membrane attack complex and perforin) domain of complements and perforin proteins that are involved in innate immunity in animals. (or.jp)
  • Differential roles of JIP scaffold proteins in the modulation of amyloid precursor protein metabolism. (nih.gov)
  • ANG II induced a significant dose-dependent increase in ACE and decrease in ACE2 mRNA and protein expression in CATH.a neurons. (nebraska.edu)
  • Gene ontology functional enrichment analysis was performed using search tool for the retrieval of interacting genes/proteins. (ijpsonline.com)
  • This study aimed to evaluate the expression levels of miR-34a and 11 of its bioinformatically selected target genes and proteins to test their potential dysregulation in RCC. (hindawi.com)
  • Activated ER binds to estrogen-responsive elements (EREs), which are located in the promoter regions of various target genes. (iiarjournals.org)
  • For the refinement of RCC therapeutic strategies, a better realization of the RCC-underlying molecular mechanisms will be mandatory [ 3 ]. (hindawi.com)
  • Molecular recognition of proteins: Sequence selective binding of aspartate pairs in helical peptides. (nyu.edu)
  • Most Ras proteins contain a lipid modification site at the C-terminus, with a typical sequence motif CaaX, where a = an aliphatic amino acid and X = any amino acid. (nih.gov)
  • Consequently, PP2A can increase the activity of caspase 3 by dual mechanisms and thereby promote the apoptotic response in human neutrophils. (lu.se)
  • Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. (origene.com)
  • The encoded kinase participates in the regulation of vascular endothelial growth factor (VEGF) expression. (wikipedia.org)
  • Furthermore, using pharmacologic technique you can inhibit proteins activity activity transiently at an accurate physiological stage. (phytid.org)
  • This protein has been shown to interact with and regulate the activity of MAPK8/JNK1 and MAP2K7/MKK7 kinases. (nih.gov)
  • Furthermore, cytochrome c (Cyt C) release, and the activity of caspase-8 and caspase-3 were also determined. (springer.com)
  • Using two different methods it was possible to detect constitutive activity of p38 mitogen-activated protein kinase (p38) in newly isolated neutrophils. (lu.se)
  • Many investigators consider the expression of estrogen-responsive genes (ERGs) to be evidence of an activated, functional ER ( 6 , 7 ). (iiarjournals.org)
  • The results in this dissertation also demonstrate that the protein phosphatase type 2A (PP2A) can directly and independently decrease the phosphorylation levels of both p38 and caspase 3. (lu.se)
  • We first identified the bioactive phytoconstituents from Amaranthus tricolor and predicted their potential protein targets involved in the pathogenesis of cognitive dysfunction using BindingDB (p≥0.7). (ijpsonline.com)
  • Thirty nine phytoconstituents were identified in Amaranthus tricolor , five of which were predicted to modulate eight potential protein targets involved in cognitive impairment. (ijpsonline.com)