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  • mice
  • Although in vivo adiponectin overexpression reduced MKP1 protein levels, the stimulative effects of adiponectin on mitochondrial biogenesis vanished in skeletal muscle of PGC-1α knockout mice. (diabetesjournals.org)
  • In addition, E6201 cream inhibited the 1-fluoro-2,4-dinitrobenzene-induced contact hypersensitivity reaction mediated by T cells in mice. (aspetjournals.org)
  • dephosphorylation
  • MKP-1 protein was also markedly up-regulated following 1-8 h of Dex treatment, and this correlated precisely with dephosphorylation of ERK. (ox.ac.uk)
  • Knockdown of MKP-1 using either immunodepletion of MKP-1 before in vitro dephosphorylation assay or short interference RNA transfection prevents inactivation of ERK by GCs. (ox.ac.uk)
  • vitro
  • Two of the pyrrole carboxamides, PSI2106 and MDF2085, were especially notable in vitro inhibitors of recombinant human MKP-1 enzyme activity with IC 50 values of 8.0 ± 0.9 and 8.3 ± 0.8 μM, respectively. (aspetjournals.org)
  • The most promiscuous of the 11 human MKPs is MKP-1, which can dephosphorylate p38, c-Jun NH 2 -terminal kinase and extracellular signaling-related protein kinase (Erk) 1/2 in vitro. (aspetjournals.org)
  • T1481fs encoded an unstable and nonfunctional protein when expressed in vitro. (nih.gov)
  • humans
  • Owing to huge tissue mass and relatively high energy demand, skeletal muscle plays a critical role in energy expenditure in humans and most rodents ( 1 , 2 ). (diabetesjournals.org)
  • Adiponectin mRNA and blood protein levels are inversely associated with obesity ( 7 ), which is a common cause of insulin resistance in humans. (diabetesjournals.org)
  • mRNA expression
  • Furthermore, anisomycin, a potent stimulus of SAPK and p38 MAP kinase, also induced MKP-1 mRNA expression. (ahajournals.org)
  • MKP-1, but not MKP-3, mRNA expression was 10-fold up-regulated in both mouse and human osteoblast cell lines within 30 min of Dex treatment and remained elevated for 24 h. (ox.ac.uk)
  • mediates
  • Mekk1 mediates p53 protein stability in the presence of Mdm2 and reduces p53 ubiquitination, suggesting an interference with Mdm2-mediated degradation of p53 by the ubiquitin-proteasome pathway. (nih.gov)
  • promoter
  • Here, we show that subcytotoxic oxidative stress generated by hydrogen peroxide application promotes premature senescence and stimulates the activity of a (−1,296) caveolin-1 promoter reporter gene construct in fibroblasts. (aacrjournals.org)
  • Functional deletion analysis mapped the oxidative stress response elements of the mouse caveolin-1 promoter to the sequences −244/−222 and −124/−101. (aacrjournals.org)
  • genes
  • The JNK interacting protein JIP-1 and insulin like growth factor II genes are co-expressed in human embryonic tumours. (nih.gov)
  • downregulation
  • 12,13 Moreover, in our recent study, we found that morphine tolerance is associated with downregulation of spinal glutamate transporters (GLAST, GLT-1, and EAAC1) and increasing of excitatory amino acids aspartate and glutamate concentration in spinal cerebrospinal fluid dialysates. (asahq.org)
  • cytokine
  • 7 Administration of glial metabolic inhibitors, cytokine antagonists, antiinflammatory cytokine interleukin (IL)-10, or IL-1 receptor antagonist IL-1ra has been shown to attenuate the development of opioid tolerance and to prevent hyperalgesia and allodynia. (asahq.org)
  • inhibitors
  • The lack of readily available selective MKP-1 inhibitors has severely limited interrogation of its biological role and was one rationale for using a recently described tricyclic pyrrole-2-carboxamide library in our screening efforts. (aspetjournals.org)